Publications

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the draft genome sequence of the plant growth promoting rhizospheric bacterium enterobacter cloacae sbp-8.here, we report the draft genome sequence and annotation of plant growth promoting rhizobacterium enterobacter cloacae sbp-8 isolated from the rhizosphere of sorghum bicolor l. growing in desert region of rajasthan, india. from the genome sequences, we identified the genes encoding plant growth promoting properties such as 1-aminocyclopropane-1-carboxylate deaminase (acds), phosphate solubilisation, siderophore, and iaa (indole acetic acid) production. the genes encoding different functions requ ...201728386532
draft genome sequence of a high-level colistin-resistant clinical strain of the enterobacter cloacae complex.strain wchecl-c4 of the enterobacter cloacae complex, recovered from the blood of a patient with peritonitis, was high-level resistant to colistin. here, we report its 5.1-mb draft genome sequence, comprising 92 contigs with an average 55.74% g+c content. the genome contained 4,783 coding sequences and 68 trna genes.201728385849
carbapenemase-producing enterobacteriaceae recovered from a spanish river ecosystem.the increasing resistance to carbapenems is an alarming threat in the fight against multiresistant bacteria. the dissemination properties of antimicrobial resistance genes are supported by their detection in a diverse population of bacteria, including strains isolated from the environment. the objective of this study was to investigate the presence of carbapenemase-producing enterobacteriaceae (cpe) collected from a river ecosystem in the barcelona metropolitan area (spain). identification of β- ...201728380016
[community acquired urinary tract infections in older adults].background community acquired urinary tract infections (cauti) in adults are common ailments. older adults are prone to infectious diseases, diagnosis can be difficult, their etiologic and antimicrobial resistance are poorly known. objectives to evaluate the incidence discriminated by sex, symptoms, and to determine pathogens and their antimicrobial resistance rate. methods a retrospective, descriptive and comparative study. we analyze uricultures among 2013 in the hospital nacional de clinicas ...201728379126
xer site specific recombination: double and single recombinase systems.the separation and segregation of newly replicated bacterial chromosomes can be constrained by the formation of circular chromosome dimers caused by crossing over during homologous recombination events. in escherichia coli and most bacteria, dimers are resolved to monomers by site-specific recombination, a process performed by two chromosomally encoded tyrosine recombinases (xerc and xerd). xercd recombinases act at a 28 bp recombination site dif, which is located at the replication terminus reg ...201728373867
gallbladder microbiota variability in colombian gallstones patients.gallbladder stones are a very frequently occurring condition. despite bile bactericidal activity, many bacteria have been detected inside the gallbladder, and gallstones facilitate their presence. between 3% and 5% of the patients with salmonella spp. infection develop the carrier stage, with the bacteria persisting inside the gallbladder, shedding bacteria in their feces without signs of infection. the aim of this study was to isolate bacteria from colombian patients with gallstones, using stan ...201728368860
efficient synthesis of tyrosol galactosides by the β-galactosidase from enterobacter cloacae b5.in this work, the β-galactosidase from enterobacter cloacae b5 (bgab5) exhibited excellent transglycosylation activity toward tyrosol (p-hydroxyphenethyl alcohol) when using lactose as the glycosyl donor, generating a series of tyrosol glycosides with potential pharmacological properties. the effects of substrate concentration, temperature, ph, and reaction time on the transglycosylation reaction catalyzed by the enzyme bgab5 were studied in detail. three tyrosol derivatives were produced in a t ...201728361236
troubled water under the bridge: screening of river mur water reveals dominance of ctx-m harboring escherichia coli and for the first time an environmental vim-1 producer in austria.antibiotic resistant bacteria (arb) in the aquatic environment are reported from all over the world and their presence in the environment has become quite common. the current most prominent example is the presence of beta-lactamases harboring enterobacteriaceae. the aim of this study was to investigate the presence and diversity (on the genetic and phenotypic levels) of extended spectrum beta-lactamases (esbl) and carbapenemases harboring enterobacteriaceae from the river mur in the center of gr ...201728351808
structural and genetic characterization of the o-antigen of enterobacter cloacae c5529 related to the o-antigen of e. cloacae g3054.on mild acid degradation of the lipopolysaccharide of enterobacter cloacae c5529, the o-polysaccharide chain was cleaved at the linkages of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (di-n-acetylpseudaminic acid, psep5ac7ac). the resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with (1)h and (13)c nmr spectroscopy, and the following structure of the tetrasaccharide repeating unit of the o-polysaccharide was esta ...201728342969
two-year follow-up outcomes of premature infants enrolled in the phase i trial of mesenchymal stem cells transplantation for bronchopulmonary dysplasia.to determine the long-term safety and outcomes of mesenchymal stem cells (mscs) for bronchopulmonary dysplasia in premature infants enrolled in a previous phase i clinical trial up to 2 years of corrected age (ca).201728341525
high prevalence of non-clonal imipenem-nonsusceptible enterobacter spp. isolates in korea and their association with porin down-regulation.we investigated the prevalence and clonal distribution of imipenem-nonsusceptible enterobacter clinical isolates from hospitals in korea and the contributions of various mechanisms to imipenem nonsusceptibility. the in vitro antimicrobial susceptibility to imipenem of 357 non-duplicated enterobacter isolates obtained from eight geographically distant tertiary care hospitals in korea was evaluated. imipenem-nonsusceptible enterobacter isolates were genotyped. additionally, β-lactamase genes were ...201728336135
soft tissue infections from fish spike wounds: normal commensal bacteria are more common than marine pathogens.a fish spike injury can be sustained by anyone handling fish; during fishing, meal preparation or in retail. case reports of fish spikes inoculating victims with virulent marine-specific pathogens and causing systemic illness led us to question whether empirical treatment of these injuries with amoxicillin and clavulanic acid is adequate.201728320052
screening for fecal carriage of mcr-producing enterobacteriaceae in healthy humans and primary care patients.the extent of the occurrence of the plasmid-encoded colistin resistance genes mcr-1 and mcr-2 among humans is currently sparsely studied in western europe.201728316780
ulcerative granuloma of the eyelid as the initial manifestation of granulomatosis with polyangiitis (wegener's granulomatosis): a case report.a 56-year-old-man presented a 2-month history of chalazion in the eyelids without response to treatment and with an inconclusive biopsy. laboratory results confirmed the presence of enterobacter cloacae and streptococcus gordonii infection. despite appropriate intravenous antibiotic treatment, clinical worsening was observed. radical surgical excision and total tarsorrhaphy were performed. following treatment, the patient was asymptomatic for 6 weeks until he developed acute renal failure, gener ...201728306365
in vitro activity of imipenem against carbapenemase-positive enterobacteriaceae: smart global surveillance program 2008-2014.the study for monitoring antimicrobial resistance trends (smart) global surveillance program collected 103,960 isolates of enterobacteriaceae from 2008 to 2014. from this isolate collection all ertapenem-non-susceptible isolates (mic, ≥1 μg/ml; n = 3,428) and 9,371 isolates of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, and proteus mirabilis with an ertapenem-susceptible extended-spectrum β-lactamase (esbl)-positive phenotype were assessed for the presence of common carbapenemas ...201728298454
does second place count? lessons from a major discrepancy between carbapenem-resistant klebsiella pneumoniae and carbapenem-resistant enterobacter cloacae in a one-year follow-up study. 201728260534
in vitro activity of ceftaroline and comparator agents against gram-positive and gram-negative clinical isolates from cancer patients.bacterial infections are common in cancer patients. ceftaroline (cft) is a broad-spectrum cephalosporin with activity against most gram-positive organisms (gpos) and many gram-negative organisms. in this study, the in vitro activity of cft was compared with vancomycin (van), daptomycin (dap), linezolid (lzd), trimethoprim/sulphamethoxazole (sxt) and tigecycline (tig) against bacteria (predominantly blood culture isolates) isolated from cancer patients in 2014 and 2015. cft was active against met ...201728257904
human norovirus binding to select bacteria representative of the human gut microbiota.recent reports describe the ability of select bacterial strains to bind human norovirus, although the specificity of such interactions is unknown. the purpose of this work was to determine if a select group of bacterial species representative of human gut microbiota bind to human norovirus, and if so, to characterize the intensity and location of that binding. the bacteria screened included naturally occurring strains isolated from human stool (klebsiella spp., citrobacter spp., bacillus spp., e ...201728257478
very high prevalence of extended-spectrum beta-lactamase-producing enterobacteriaceae in bacteriemic patients hospitalized in teaching hospitals in bamako, mali.the worldwide dissemination of extended-spectrum beta-lactamase producing enterobacteriaceae, (esbl-e) and their subset producing carbapenemases (cpe), is alarming. limited data on the prevalence of such strains in infections from patients from sub-saharan africa are currently available. we determined, here, the prevalence of esbl-e/cpe in bacteriemic patients in two teaching hospitals from bamako (mali), which are at the top of the health care pyramid in the country. during one year, all entero ...201728245252
aerobic and heterotrophic nitrogen removal by enterobacter cloacae cf-s27 with efficient utilization of hydroxylamine.heterotrophic bacterium, enterobacter cloacae cf-s27 exhibited simultaneous nitrification and aerobic denitrification in presence of high concentration of hydroxylamine. with the initial nitrogen concentration of 100mgl(-1)h(-1), ammonium, nitrate and nitrite removal efficiencies were 81%, 99.9% and 92.8%, while the corresponding maximum removal rates reached as high as 11.6, 15.1 and 11.2mgl(-1)h(-1) respectively. quantitative amplification by real time pcr and enzyme assay demonstrated that hy ...201728242385
enterobacter cloacae complex isolates harboring blanmc-a or blaimi-type class a carbapenemase genes on novel chromosomal integrative elements and plasmids.carbapenem-resistant enterobacter cloacae complex isolates submitted to a reference laboratory from 2010 to 2015 were screened by pcr for seven common carbapenemase gene groups, namely, kpc, ndm, oxa-48, vim, imp, ges, and nmc-a/imi. nineteen of the submitted isolates (1.7%) were found to harbor ambler class a blanmc-a or blaimi-type carbapenemases. all 19 isolates were resistant to at least one carbapenem but susceptible to aminoglycosides, trimethoprim-sulfamethoxazole, tigecycline, and ciprof ...201728223374
[etiologic diagnosis in meningitis and encephalitis molecular biology techniques].the aetiological study of infections of the central nervous system has traditionally been performed using bacterial cultures and, more recently, using polymerase chain reaction (pcr) for herpes simplex virus (hsv). bacterial cultures may not have good performance, especially in the context of patients who have received antibiotics prior to sampling, and a request for hsv only by pcr reduces the information to only one aetiological agent. the aim of this study is to determine the infectious cause ...201726493774
high-quality genome sequence of human pathogen enterobacter asburiae type strain 1497-78(t).enterobacter asburiae belongs to the enterobacter cloacae complex (ecc), which comprises six heterogenic species. these bacteria can cause nosocomial infections. moreover, they are well known for antibiotic resistance features based on overproduction of ampc β-lactamases. although ecc have clinical importance, little is known about their virulence-associated properties, and very few strains from the six species have been sequenced. in this study, the type strain of e. asburiae 1497-78(t) (atcc 3 ...201728082144
prevalence and antimicrobial susceptibility of enterobacteriaceae isolated from retail pepper in vietnam.to investigate the microbial quality of retail pepper in vietnam, the enumeration and detection of enterobacteriaceae and the screening of cefotaxime (ctx)-resistant coliforms were performed by using 84 commercial samples. although enterobacteriaceae were isolated from 78 samples, the number of enterobacteriaceae was lower than 1.0 log cfu/g in 46 samples. for the detection of enterobacteriaceae with the international organization for standardization methods, salmonella spp., escherichia coli , ...201728350183
risk of infection transmission in curvilinear array echoendoscopes: results of a prospective reprocessing and culture registry.the complex design of the elevator mechanism in duodenoscopes has been recognized as a challenge for disinfection and recently implicated as a potential source of persistent bacterial contamination. curvilinear array (cla) echoendoscopes also have an elevator mechanism; however, there are no recommendations or data regarding the risk of persistent bacterial contamination of echoendoscopes. here we hoped to determine the yield of microbial growth with routine bacterial surveillance cultures of re ...201727478029
incn st7 epidemic plasmid carrying blaimp-4 in enterobacteriaceae isolates with epidemiological links to multiple geographical areas in china.to characterize blaimp-4-carrying plasmids originating from inpatients in hong kong.201727609049
prospective surveillance of healthcare associated infections in a cambodian pediatric hospital.healthcare associated infections (hai) are the most common preventable adverse events following admission to healthcare facilities. data from low-income countries are scarce. we sought to prospectively define hai incidence at angkor hospital for children (ahc), a cambodian pediatric referral hospital.201728138385
evaluation of three sample preparation methods for the direct identification of bacteria in positive blood cultures by maldi-tof.patient mortality is significantly reduced by rapid identification of bacteria from sterile sites. maldi-tof can identify bacteria directly from positive blood cultures and multiple sample preparation methods are available. we evaluated three sample preparation methods and two maldi-tof score cut-off values. positive blood culture bottles with organisms present in gram stains were prospectively analysed by maldi-tof. three lysis reagents (saponin, sds, and sepsityper lysis bufer) were applied to ...201728100271
whole genome sequencing reveals high-resolution epidemiological links between clinical and environmental klebsiella pneumoniae.klebsiella pneumoniae is a gram-negative bacterial species capable of occupying a broad range of environmental and clinical habitats. known as an opportunistic pathogen, it has recently become a major causative agent of clinical infections worldwide. despite growing knowledge about the highly diverse population of k. pneumoniae, the evolution and clinical significance of environmental k. pneumoniae, as well as the relationship between clinical and environmental k. pneumoniae, are poorly defined.201728118859
development of an algorithm for phenotypic screening of carbapenemase-producing enterobacteriaceae in the routine laboratory.carbapenemase-producing enterobacteriaceae (cpe) are difficult to identify among carbapenem non-susceptible enterobacteriaceae (nse). we designed phenotypic strategies giving priority to high sensitivity for screening putative cpe before further testing.201728095794
the influence of antibiotic prophylaxis on bacterial resistance in urinary tract infections in children with spina bifida.bacterial resistance to antibiotics is an increasingly threatening consequence of antimicrobial exposure for many decades now. in urinary tract infections (utis), antibiotic prophylaxis (ap) increases bacterial resistance. we studied the resistance patterns of positive urinary cultures in spina bifida children on clean intermittent catheterization, both continuing and stopping ap.201728081719
proceedings of réanimation 2017, the french intensive care society international congress. 201728074416
proceedings of réanimation 2017, the french intensive care society international congress. 201728074415
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