Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| struct2net: a web service to predict protein-protein interactions using a structure-based approach. | struct2net is a web server for predicting interactions between arbitrary protein pairs using a structure-based approach. prediction of protein-protein interactions (ppis) is a central area of interest and successful prediction would provide leads for experiments and drug design; however, the experimental coverage of the ppi interactome remains inadequate. we believe that struct2net is the first community-wide resource to provide structure-based ppi predictions that go beyond homology modeling. a ... | 2010 | 20513650 |
| alpha4 phosphoprotein interacts with edd e3 ubiquitin ligase and poly(a)-binding protein. | mammalian alpha4 phosphoprotein, the homolog of yeast tap42, is a component of the mammalian target-of-rapamycin (mtor) pathway that regulates ribogenesis, the initiation of translation, and cell-cycle progression. alpha4 is known to interact with the catalytic subunit of protein phosphatase 2a (pp2ac) and to regulate pp2a activity. using alpha4 as bait in yeast two-hybrid screening of a human k562 erythroleukemia cdna library, edd (e3 isolated by differential display) e3 ubiquitin ligase was id ... | 2010 | 20544796 |
| expression, purification and characterization of the cancer-germline antigen gage12i: a candidate for cancer immunotherapy. | gage cancer-germline antigens are frequently expressed in a broad range of different cancers, while their expression in normal tissues is limited to the germ cells of the immune privileged organs, testis and ovary. gage proteins are immunogenic in humans, which make them promising targets for immunotherapy and candidates for cancer vaccines. recombinant proteins may be superior to peptides as immunogens, since they have the potential to prime both cd4(+) and cd8(+) t cells and are not dependent ... | 2010 | 20546897 |
| telomeres: structures in need of unwinding. | telomeres protect the ends of eukaryotic chromosomes from being recognized and processed as double strand breaks. in most organisms, telomeric dna is highly repetitive with a high gc-content. moreover, the g residues are concentrated in the strand running 3'-5' from the end of the chromosome towards its center. this g-rich strand is extended to form a 3' single-stranded tail that can form unusual secondary structures such as t-loops and g-quadruplex dna. both the duplex repeats and the single-st ... | 2010 | 20637196 |
| estrogenic pyrethroid pesticides regulate expression of estrogen receptor transcripts in mouse sertoli cells differently from 17beta-estradiol. | studies suggested that exposure to agricultural pesticides may affect male fertility. pyrethroids are widely used pesticides due to their insecticidal potency and low mammalian toxicity. a recombinant yeast assay system incorporating the human alpha-estrogen receptor was used to analyze the estrogenicity of a range of readily available pyrethroid pesticides. the commercial product ripcord plus showed estrogenic activity by this assay. to determine whether pyrethroid compounds might exert an effe ... | 2010 | 20574910 |
| predicting genetic modifier loci using functional gene networks. | most phenotypes are genetically complex, with contributions from mutations in many different genes. mutations in more than one gene can combine synergistically to cause phenotypic change, and systematic studies in model organisms show that these genetic interactions are pervasive. however, in human association studies such nonadditive genetic interactions are very difficult to identify because of a lack of statistical power--simply put, the number of potential interactions is too vast. one appro ... | 2010 | 20538624 |
| intramembrane proteolysis of mgm1 by the mitochondrial rhomboid protease is highly promiscuous regarding the sequence of the cleaved hydrophobic segment. | rhomboids are a family of intramembrane serine proteases that are conserved in bacteria, archaea, and eukaryotes. they are required for numerous fundamental cellular functions such as quorum sensing, cell signaling, and mitochondrial dynamics. mitochondrial rhomboids form an evolutionarily distinct class of rhomboids. it is largely unclear how their activity is controlled and which substrate determinants are responsible for recognition and cleavage. we investigated these requirements for the mit ... | 2010 | 20558178 |
| secreted ph-regulated antigen 1 of candida albicans blocks activation and conversion of complement c3. | the complement system forms the first defense line of innate immunity and is activated within seconds upon infection by human pathogenic yeast candida albicans. in this study, we identified a new complement evasion strategy used by c. albicans. the fungus secretes a potent complement inhibitor, ph-regulated ag 1 (pra1), which in the direct surrounding of the pathogen binds to fluid-phase c3 and blocks cleavage of c3 to c3a and c3b, as shown by elisa, native gel electrophoresis, and western blott ... | 2010 | 20644161 |
| combining machine learning and homology-based approaches to accurately predict subcellular localization in arabidopsis. | a complete map of the arabidopsis (arabidopsis thaliana) proteome is clearly a major goal for the plant research community in terms of determining the function and regulation of each encoded protein. developing genome-wide prediction tools such as for localizing gene products at the subcellular level will substantially advance arabidopsis gene annotation. to this end, we performed a comprehensive study in arabidopsis and created an integrative support vector machine-based localization predictor ... | 2010 | 20647376 |
| [lass2 interacts with v-atpase and inhibits cell growth of hepatocellular carcinoma]. | homo sapiens longevity assurance homologue 2 (lass2) is a novel gene isolated from a human liver cdna library by our laboratory, and it is a human homologue of the yeast longevity assurance gene lag1 (saccharomyces cerevisiae longevity assurance gene). according to our previous results, lass2 could interact with subunit c of vacuolar type h(+)-atpase (v-atpase), and the overexpression of lass2 could inhibit the cell growth of a human hepatocellular carcinoma (hcc) cell line, smmc-7721. in order ... | 2010 | 20571735 |
| [screening and identification of interactive proteins of sh2d4a]. | sh2d4a is a member of sh2 signaling protein family, which is involved in the signal transduction mediated by protein tyrosine kinase-related receptor, cell growth, proliferation, differentiation, and thereby affects the development of human disorders. to determine the role of sh2d4a in the cell signal transduction pathway, sh2d4a interactive proteins were screened using yeast two-hybrid system, and yeast mating and gst pull-down assays were carried out to further confirm the interaction. we succ ... | 2010 | 20650852 |
| streptococcus mutans inhibits candida albicans hyphal formation by the fatty acid signaling molecule trans-2-decenoic acid (sdsf). | in the human mouth, fungi and several hundred species of bacteria coexist. here we report a case of interkingdom signaling in the oral cavity: a compound excreted by the caries bacterium streptococcus mutans inhibits the morphological transition from yeast to hyphae, an important virulence trait, in the opportunistic fungus candida albicans. the compound excreted by s. mutans was originally studied because it inhibited signaling by the universal bacterial signal autoinducer-2 (ai-2), determined ... | 2010 | 20572249 |
| the cellular rna helicase ddx1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. | the involvement of host proteins in the replication and transcription of viral rna is a poorly understood area for many rna viruses. for coronaviruses, it was long speculated that replication of the giant rna genome and transcription of multiple subgenomic mrna species by a unique discontinuous transcription mechanism may require host cofactors. to search for such cellular proteins, yeast two-hybrid screening was carried out by using the nonstructural protein 14 (nsp14) from the coronavirus infe ... | 2010 | 20573827 |
| the closely related rna helicases, uap56 and urh49, preferentially form distinct mrna export machineries and coordinately regulate mitotic progression. | nuclear export of mrna is an essential process for eukaryotic gene expression. the trex complex couples gene expression from transcription and splicing to mrna export. sub2, a core component of the trex complex in yeast, has diversified in humans to two closely related rna helicases, uap56 and urh49. here, we show that urh49 forms a novel urh49-cip29 complex, termed the arex (alternative mrna export) complex, whereas uap56 forms the human trex complex. the mrnas regulated by these helicases are ... | 2010 | 20573985 |
| serine-409 phosphorylation and oxidative damage define aggregation of human protein tau in yeast. | unraveling the biochemical and genetic alterations that control the aggregation of protein tau is crucial to understand the etiology of tau-related neurodegenerative disorders. we expressed wild type and six clinical frontotemporal dementia with parkinsonism (ftdp) mutants of human protein tau in wild-type yeast cells and cells lacking mds1 or pho85, the respective orthologues of the tau kinases gsk3β and cdk5. we compared tau phosphorylation with the levels of sarkosyl-insoluble tau (sint), as ... | 2010 | 20662935 |
| control of vitellogenin genes expression by sequences derived from transposable elements in rainbow trout. | in most of oviparous animals, vitellogenins (vtg) are the major egg yolk precursors. they are produced in the liver under the control of estrogens. in rainbow trout (oncorhynchus mykiss), the vtg genes cluster contains an unusually large number of almost identical gene copies. in order to identify the regulatory elements in their promoters, we used a combination of reporter plasmids containing genomic sequences including putative estrogen response elements (eres) and we performed transient trans ... | 2010 | 20637911 |
| long-term conservation of hcv rna at 4 degrees c using a new rna stabilizing solution. | protecting rna from degradation, whilst maintaining its biological activity, is essential in molecular biology. however, rna is very sensitive to degradation by ribonucleases, especially at temperatures above 0 degrees c. the stability of rna was examined at 4 degrees c and -20 degrees c, in a new stabilizing solution consisting of a low-molarity mixture of chaotropic agents guanidinium and ammonium thiocyanate, a buffer for ph stabilization, phenol, and yeast rna. two substrates were tested for ... | 2010 | 20576506 |
| simple is good: yeast models of neurodegeneration. | the budding yeast, saccharomyces cerevisiae, is the best-studied eukaryotic cell, at both genetic and physiological levels. as a eukaryote, yeast shares highly conserved molecular and cellular mechanisms with human cells. thus, this simple fungus is an invaluable model to study the fundamental molecular mechanisms involved in several human diseases. in the particular case of neurodegenerative disorders, yeast models have been able to recapitulate several important features of complex and devasta ... | 2010 | 20579105 |
| identification of binding domains in the herpes simplex virus type 1 small capsid protein pul35 (vp26). | in this study, fragments of the small capsid protein pul35 (vp26) from herpes simplex virus type 1 (hsv-1) were generated to identify binding domains for a number of known ligands. analysis of the binding of dynein light chain subunits, dynlt1 and dynlt3, as well the hsv-1 structural proteins pul19 (vp5) and pul37 was then undertaken using the lexa yeast two-hybrid assay. the n-terminal half of pul35, in particular residues 30-43, was identified as a common region for the binding of dynlt1 and d ... | 2010 | 20668116 |
| antimicrobial activity of mucosal-associated invariant t cells. | mucosal-associated invariant t lymphocytes (mait lymphocytes) are characterized by two evolutionarily conserved features: an invariant t cell antigen receptor (tcr) alpha-chain and restriction by the major histocompatibility complex (mhc)-related protein mr1. here we show that mait cells were activated by cells infected with various strains of bacteria and yeast, but not cells infected with virus, in both humans and mice. this activation required cognate interaction between the invariant tcr and ... | 2010 | 20581831 |
| defining the pathway of cytoplasmic maturation of the 60s ribosomal subunit. | in eukaryotic cells the final maturation of ribosomes occurs in the cytoplasm, where trans-acting factors are removed and critical ribosomal proteins are added for functionality. here, we have carried out a comprehensive analysis of cytoplasmic maturation, ordering the known steps into a coherent pathway. maturation is initiated by the atpase drg1. downstream, assembly of the ribosome stalk is essential for the release of tif6. the stalk recruits gtpases during translation. because the gtpase ef ... | 2010 | 20670889 |
| the anaphase promoting complex activator ccs52a, a key factor for fruit growth and endoreduplication in tomato. | tomato fruit growth is characterized by the occurrence of numerous rounds of dna endoreduplication in connection to cell expansion and final fruit size determination. endoreduplication occurs as an impairment of mitosis, which can originate from the selective degradation of m-phase-specific cyclins via the ubiquitin-mediated proteolytic pathway, requiring the e3 ubiquitin ligase anaphase promoting complex/cyclosome (apc/c). in plants ccs52a is the ortholog of cdh1/fzr proteins from yeast, drosop ... | 2010 | 20671429 |
| identification of the transactivation domain of the human fhl3. | four and a half lim domain protein 3 (fhl3) has the transactivation and repressor activity, and plays important roles in regulating the expression levels of various genes. in this study, fhl3 was proved to possess the auto-activation ability when constructed into the pgbkt7 plasmid (a gala dna-binding domain (bd) cloning vector of the yeast two-hybrid system) and transformed into yeast y190 cells. to determine the transactivation domain of fhl3, five mutants were constructed by sequentially dele ... | 2010 | 20586194 |
| functional characterization of aibp, a novel aurora-a binding protein in centrosome structure and spindle formation. | aurora-a is involved in chromosome alignment, centrosome maturation, mitotic spindle assembly and regards to an oncogene. aurora-a is also known to bind to several other proteins affecting its up-regulation or down-regulation and localization. however, how these different binding signals work together to regulate aurora-a is not properly known. to explore more aurora-a interacting proteins, the low-copy yeast two-hybrid screening using aurora-a as bait protein was performed. one novel gene, aibp ... | 2010 | 20596670 |
| enterovirus type 71 2a protease functions as a transcriptional activator in yeast. | enterovirus type 71 (ev71) 2a protease exhibited strong transcriptional activity in yeast cells. the transcriptional activity of 2a protease was independent of its protease activity. ev71 2a protease retained its transcriptional activity after truncation of 40 amino acids at the n-terminus but lost this activity after truncation of 60 amino acids at the n-terminus or deletion of 20 amino acids at the c-terminus. thus, the acidic domain at the c-terminus of this protein is essential for its trans ... | 2010 | 20682079 |
| structure-mutagenicity relationship of kaurenoic acid from xylopia sericeae (annonaceae). | kaurane diterpenes are considered important compounds in the development of new highly effective anticancer chemotherapeutic agents. genotoxic effects of anticancer drugs in non-tumour cells are of special significance due to the possibility that they induce secondary tumours in cancer patients. in this context, we evaluated the genotoxic and mutagenic potential of the natural diterpenoid kaurenoic acid (ka), i.e. (-)-kaur-16-en-19-oic acid, isolated from xylopia sericeae st. hill, using several ... | 2010 | 20599626 |
| a decade of systems biology. | systems biology provides a framework for assembling models of biological systems from systematic measurements. since the field was first introduced a decade ago, considerable progress has been made in technologies for global cell measurement and in computational analyses of these data to map and model cell function. it has also greatly expanded into the translational sciences, with approaches pioneered in yeast now being applied to elucidate human development and disease. here, we review the sta ... | 2010 | 20604711 |
| the emerging role of vhs domain-containing tom1, tom1l1 and tom1l2 in membrane trafficking. | the maintenance of cellular homeostasis and execution of regulatory mechanisms to dynamically govern various cellular processes require the correct delivery of proteins to their target subcellular compartments. it is estimated that over 30% of the proteins encoded by the human genome, projected to encode about 25 000 proteins and other macromolecules, are delivered to the secretory and endocytic pathways where movement of proteins between various compartments is primarily mediated by vesicles/ca ... | 2010 | 20604899 |
| the saccharomyces cerevisiae swi/snf complex can catalyze formation of dimeric nucleosome structures in vitro. | the swi/snf chromatin-remodeling complexes, human baf/pbaf and yeast rsc, can catalyze formation of stably altered dimeric forms of nucleosomes. however, the ability to create remodeled dimers has not yet been reported for the saccharomyces cerevisiae swi/snf complex. despite its similarity with the other swi/snf proteins, the yeast swi/snf complex features certain structural and functional differences. this raises the question of whether yswi/snf can in fact catalyze formation of dimeric nucleo ... | 2010 | 20608642 |
| purification of functional human cl(-)/hco(3)(-) exchanger, ae1, over-expressed in saccharomyces cerevisiae. | there is no high-resolution structure for the membrane domain of the human erythrocyte anion exchanger, ae1 (band 3). in this report, we have developed an expression and purification strategy for ae1 to be used in crystallization trials. saccharomyces cerevisiae strain bj5457 was transformed with an expression vector encoding the ae1 membrane domain (ae1md, amino acids 388-911), fused c-terminally to an epitope tag, corresponding to the nine c-terminal amino acids of rhodopsin. the fusion protei ... | 2010 | 20609390 |
| relocation of the chromosomal passenger complex prevents mitotic checkpoint engagement at anaphase. | the mitotic checkpoint monitors the attachment of kinetochores to microtubules and delays anaphase onset until all sister kinetochores have become attached to opposite poles [1, 2]. correct bipolar attachment leads to kinetochore deformation and tension and satisfies the checkpoint [3-6]. what prevents mitotic checkpoint reactivation when sister centromeres are split and tension is lost at anaphase onset? aurora b kinase, the catalytic subunit of the chromosomal passenger protein complex (cpc) [ ... | 2010 | 20619651 |
| post-translational control of ets transcription factors: detection of modified factors at target gene promoters. | ets transcription factors are implicated in gene regulation during cell proliferation and in the development of the haematopoietic cell lineage. characteristically, ets proteins act in concert with other transcription factors and are regulated by post-translational modifications, most frequently phosphorylation. these events have been shown to modulate the dna binding affinity and interactions of ets transcription factors with co-activators, events that can ultimately determine the formation of ... | 2010 | 20694674 |
| a screening method tuned for mrna processing factors in human cells by evaluation of the luciferase reporter activity and the subcellular distribution of bulk poly(a)+ rna. | screening of mrna export factors in saccharomyces cerevisiae and drosophila melanogaster has identified a number of mrna processing factors involved in multiple mrna processing steps. however, only limited information is available on human cells. here we established a screening system searching for mrna processing factors in human cells by combining the luciferase reporter system and fluorescence in situ hybridization, which evaluates the nuclear/cytoplasmic distribution of bulk poly(a)+ rna. th ... | 2010 | 20622428 |
| the collagen v homotrimer [alpha1(v)](3) production is unexpectedly favored over the heterotrimer [alpha1(v)](2)alpha2(v) in recombinant expression systems. | collagen v, a fibrillar collagen with important functions in tissues, assembles into distinct chain associations. the most abundant and ubiquitous molecular form is the heterotrimer [alpha1(v)](2)alpha2(v). in the attempt to produce high levels of recombinant collagen v heterotrimer for biomedical device uses, and to identify key factors that drive heterotrimeric chain association, several cell expression systems (yeast, insect, and mammalian cells) have been assayed by cotransfecting the human ... | 2010 | 20625483 |
| evolution of cst function in telomere maintenance. | telomeres consist of an elaborate, higher-order dna architecture, and a suite of proteins that provide protection for the chromosome terminus by blocking inappropriate recombination and nucleolytic attack. in addition, telomeres facilitate telomeric dna replication by physical interactions with telomerase and the lagging strand replication machinery. the prevailing view has been that two distinct telomere capping complexes evolved, shelterin in vertebrates and a trimeric complex comprised of cdc ... | 2010 | 20697207 |
| the classical swine fever virus n-terminal protease n(pro) binds to cellular hax-1. | the positive-stranded rna genome of classical swine fever virus (csfv) encodes 12 known proteins. the first protein to be translated is the n-terminal protease (n(pro)). n(pro) helps evade the innate interferon response by targeting interferon regulatory factor-3 for proteasomal degradation and also participates in the evasion of dsrna-induced apoptosis. to elucidate the mechanisms by which n(pro) functions, we performed a yeast two-hybrid screen in which the anti-apoptotic protein hax-1 was ide ... | 2010 | 20631090 |
| mumps virus small hydrophobic protein targets ataxin-1 ubiquitin-like interacting protein (ubiquilin 4). | the small hydrophobic (sh) protein of mumps virus has been reported to interfere with innate immunity by inhibiting tumour necrosis factor alpha-mediated apoptosis. in a yeast two-hybrid screen we have identified the ataxin-1 ubiquitin-like interacting protein (a1up) as a cellular target of the sh protein. a1up contains an amino-terminal ubiquitin-like (ubl) domain, a carboxy-terminal ubiquitin-associated (uba) domain and two stress-inducible heat shock chaperonin-binding (sti1) motifs. this pla ... | 2010 | 20702650 |
| application of stopped-flow kinetics methods to investigate the mechanism of action of a dna repair protein. | transient kinetic analysis is indispensable for understanding the workings of biological macromolecules, since this approach yields mechanistic information including active site concentrations and intrinsic rate constants that govern macromolecular function. in case of enzymes, for example, transient or pre-steady state measurements identify and characterize individual events in the reaction pathway, whereas steady state measurements only yield overall catalytic efficiency and specificity. indiv ... | 2010 | 20357752 |
| identification of cysteine-rich epidermal growth factor-like domain 1alpha (creld1alpha) as a novel alpha1a-adrenoceptor-down-regulating protein and establishment of an alpha1l-adrenoceptor-expressing cell line. | two distinct alpha(1)-adrenoceptor phenotypes (alpha(1a)- and alpha(1l)-ars) are known to originate from a single adra1a(alpha(1a)) gene by an as-yet-unknown mechanism. we hypothesized that an alpha(1a)-ar-interacting protein could generate the alpha(1l)-ar phenotype and we sought to identify such a protein and to examine its effects on the expression of alpha(1a) and alpha(1l) phenotypes. cysteine-rich epidermal growth factor-like domain 1alpha (creld1alpha) was first identified using a yeast t ... | 2010 | 20508391 |
| the bidirectional cytomegalovirus immediate/early promoter is regulated by hog1 and the stress transcription factors sko1 and hot1 in yeast. | the work presented here intends to address the question of whether the immediate/early promoter of cytomegalovirus (cmv), which is widely used for expressing transgenes in eukaryotic cells, yields a constitutive expression of the transgenes under stress conditions in saccharomyces cerevisiae cells. this information would also be relevant because in the tetracycline-regulated expression (teto) system, which is one of the first choices for studying gene function from yeast to human cells, the cmv ... | 2010 | 20364387 |
| characterizing the interaction between the rab6 gtpase and mint3 via flow cytometry based fret analysis. | in extension to previously applied techniques like yeast two-hybrid and gst pull-down assays, we successfully established a facs-based fret analysis to investigate the interaction of the mint3 adaptor protein and the small rab gtpase rab6a in living mammalian cells. a mint3 mutant containing only the ptb domain (mint3delta6) is able to interact with the constitutively active form of rab6a. mint3delta4, a mutant lacking part of the ptb domain was unable to interact with rab6a in gst pull-down ana ... | 2010 | 20447381 |
| genome-wide analysis of helicase gene family from rice and arabidopsis: a comparison with yeast and human. | helicases are motor proteins which can catalyze the unwinding of stable rna or dna duplex utilizing mainly atp as source of energy. in this study we have identified complete sets of helicases from rice and arabidopsis. the helicase gene family in rice and arabidopsis contains 115 and 113 genes respectively. these helicases were validated based on their annotations and supported with organization of conserved helicase signature motifs. we have also identified homologs of 64 rice rna and dna helic ... | 2010 | 20383562 |
| bat3 promotes the membrane integration of tail-anchored proteins. | the membrane integration of tail-anchored proteins at the endoplasmic reticulum (er) is post-translational, with different tail-anchored proteins exploiting distinct cytosolic factors. for example, mammalian trc40 has a well-defined role during delivery of tail-anchored proteins to the er. although its saccharomyces cerevisiae equivalent, get3, is known to function in concert with at least four other components, get1, get2, get4 and get5 (mdy2), the role of additional mammalian proteins during t ... | 2010 | 20516149 |
| integrated phosphoproteomics analysis of a signaling network governing nutrient response and peroxisome induction. | phosphorylation of proteins is a key posttranslational modification in cellular signaling, regulating many aspects of cellular responses. we used a quantitative, integrated, phosphoproteomics approach to characterize the cellular responses of the yeast saccharomyces cerevisiae to the fatty acid oleic acid, a molecule with broad human health implications and a potent inducer of peroxisomes. a combination of cryolysis and urea solubilization was used to minimize the opportunity for reorientation o ... | 2010 | 20395639 |
| [polar steroids from solaster endeca starfish and the physiological activity of polar steroids from three starfish species]. | four polyhydroxylated steroids, new (20r)-5alpha-cholestan-3beta,6alpha,8,15alpha,24,26-hexaol (i) and known (20r,25s)-5alpha-cholestan-3beta6alpha,8,15beta,16beta,26-hexaol, (20r,25s)-5alpha-cholestan-3beta,6alpha,15beta,16beta,26-pentaol, and marthasterone sulfate were isolated from the solaster endeca starfish inhabiting the sea of okhotsk and characterized. steroid (i) contains a 24,26-dihydroxylated side chain, which is uncommon for starfish polyols. the isolated steroids and related metabo ... | 2010 | 20531484 |
| concomitant mutations in the p450 oxidoreductase and androgen receptor genes presenting with 46,xy disordered sex development and androgenization at adrenarche. | undervirilization in males, i.e. 46,xy disordered sex development (46,xy dsd), is commonly caused by either lack of androgen action due to mutant androgen receptor (ar) or deficient androgen synthesis, e.g. due to mutations in 17alpha-hydroxylase (cyp17a1). like all other microsomal cytochrome p450 (cyp) enzymes, cyp17a1 requires electron transfer from p450 oxidoreductase (por). | 2010 | 20410220 |
| biological diversity of saccharomyces yeasts of spontaneously fermenting wines in four wine regions: comparative genotypic and phenotypic analysis. | combination of molecular genetic analysis (karyotyping, pcr-rflp of met2, the its1-its2 region and the nts region) and physiological examination (melibiose and mannitol utilization, sugar-, ethanol- and copper tolerance, killer activity, fermentation vigor and production of metabolites) of yeasts isolated from spontaneously fermenting wines in four wine regions revealed very high diversity in the saccharomyces cerevisiae populations. practically each s. cerevisiae isolate showed a unique pattern ... | 2010 | 20413169 |
| (1,3)-beta-glucans activate both dectin-1 and nlrp3 inflammasome in human macrophages. | beta-glucans are naturally occurring polysaccharides that are the major cell wall components of fungi. recognition of beta-glucans is mediated through a membrane-bound pattern recognition receptor called dectin-1, and gene knock-out studies have shown that dectin-1 plays an important role in antifungal immune response in vivo. in this report, we have studied the effect of large particulate (1,3)-beta-glucans, including curdlan, glucan from baker's yeast, paramylon, and zymosan, on inflammatory r ... | 2010 | 20421639 |
| nsc126188, a piperazine alkyl derivative, induces apoptosis via upregulation of rhob in hela cells. | we describe here a piperazine alkyl derivative, nsc126188, which induced apoptosis of hela cells by upregulating rhob expression. nsc126188 caused multi-septation of fission yeast and hypersensitized a rho3 mutant, which implicates the involvement of functional human homolog rhob. the treatment of cells with nsc126188 induced apoptosis and a dramatic increase in rhob expression. in addition, rhob knockdown using sirna rescued cells from apoptosis, indicating a crucial role of rhob in nsc126188-i ... | 2010 | 20432054 |
| the c-terminal alpha-alpha superhelix of pat is required for mrna decapping in metazoa. | pat proteins regulate the transition of mrnas from a state that is translationally active to one that is repressed, committing targeted mrnas to degradation. pat proteins contain a conserved n-terminal sequence, a proline-rich region, a mid domain and a c-terminal domain (pat-c). we show that pat-c is essential for the interaction with mrna decapping factors (i.e. dcp2, edc4 and lsm1-7), whereas the p-rich region and mid domain have distinct functions in modulating these interactions. dcp2 and e ... | 2010 | 20543818 |
| the effects of selenium supplementation on the spontaneously occurring fibroid tumors of oviduct, 8-hydroxy-2'-deoxyguanosine levels, and heat shock protein 70 response in japanese quail. | oxidative stress is known to be an important contributing factor in many chronic diseases. spontaneously occurring benign oviduct leiomyomas are common tumors of the japanese quail (coturnix coturnix japonica), which makes it a good animal model for screening potential agents for testing in the prevention and treatment of human myoma uteri. since dietary intake of selenium has been associated with a reduced risk of a variety of human cancers, we investigated the effects of selenium supplementati ... | 2010 | 20432170 |
| from protein interaction profile to functional assignment: the human protein ki-1/57 is associated with pre-mrna splicing events. | the mapping of protein-protein interactions of a determined organism is considered fundamental to assign protein function in the post-genomic era. as part of this effort, screenings for pairwise interactions by yeast two-hybrid system have been used popularly to reveal protein interaction networks in different biological systems. through the identification of protein interaction partners we have successfully obtained interesting functional clues for ki-1/57, a human protein with no previous func ... | 2010 | 20436279 |
| the production, characterisation and enhanced pharmacokinetics of scfv-albumin fusions expressed in saccharomyces cerevisiae. | an expression system is described for the production of monomeric scfvs and scfv antibody fragments genetically fused to human albumin (at either the n- or c-terminus or both). based upon strains of saccharomyces cerevisiae originally developed for the production of a recombinant human albumin (recombumin) this system has delivered high levels of secreted product into the supernatant of shake flask and high cell density fed-batch fermentations. specific binding to the corresponding ligand was de ... | 2010 | 20546898 |
| the sarcomeric z-disc component myopodin is a multiadapter protein that interacts with filamin and alpha-actinin. | here we introduce myopodin as a novel filamin c binding partner. corroborative yeast two-hybrid and biochemical analyses indicate that the central part of myopodin that shows high homology to the closely related protein synaptopodin and that is common to all its currently known or predicted variants interacts with filamin c immunoglobulin-like domains 20-21. a detailed characterization of the previously described interaction between myopodin and alpha-actinin demonstrates for the first time that ... | 2010 | 20554076 |
| rapid identification of candida albicans in blood by combined capillary electrophoresis and fluorescence in situ hybridization. | a ce method based on whole-cell molecular labeling via fluorescence in situ hybridization was developed for the detection of candida albicans in whole blood. removal of potentially interfering red blood cells (rbc) with a simple hypotonic/detergent lysis step enabled us to detect and quantitate contaminating c. albicans cells at concentrations that were orders of magnitude lower than background rbc counts ( approximately 7.0 x 10(9) rbc/ml). in the presence of the lysed blood matrix, yeast cells ... | 2010 | 20665522 |
| dietary modulation of drosophila sleep-wake behaviour. | a complex relationship exists between diet and sleep but despite its impact on human health, this relationship remains uncharacterized and poorly understood. drosophila melanogaster is an important model for the study of metabolism and behaviour, however the effect of diet upon drosophila sleep remains largely unaddressed. | 2010 | 20706579 |
| identification and biophysical assessment of the molecular recognition mechanisms between the human haemopoietic cell kinase src homology domain 3 and alg-2-interacting protein x. | sfks (src family kinases) are central regulators of many signalling pathways. their functions are tightly regulated through sh (src homology) domain-mediated protein-protein interactions. a yeast two-hybrid screen using sh3 domains as bait identified alix [alg-2 (apoptosis-linked gene 2)-interacting protein x] as a novel hck (haemopoietic cell kinase) sh3 domain interactor. the alix-hck-sh3 interaction was confirmed in vitro by a gst (glutathione transferase) pull-down assay and in intact cells ... | 2010 | 20670214 |
| ny-eso-1 protein glycosylated by yeast induces enhanced immune responses. | vaccine strategies that target dendritic cells to elicit potent cellular immunity are the subject of intense research. here we report that the genetically engineered yeast saccharomyces cerevisiae, expressing the full-length tumour-associated antigen ny-eso-1, is a versatile host for protein production. exposing dendritic cells (dcs) to soluble ny-eso-1 protein linked to the yeast a-agglutinin 2 protein (aga2p) protein resulted in protein uptake, processing and mhc class i cross-presentation of ... | 2010 | 20672253 |
| construction of an in vitro trans-sialylation system: surface display of corynebacterium diphtheriae sialidase on saccharomyces cerevisiae. | sialidases can be used to transfer sialic acids from sialoglycans to asialoglycoconjugates via the trans-glycosylation reaction mechanism. some pathogenic bacteria decorate their surfaces with sialic acids which were often scavenged from host sialoglycoconjugates using their surface-localized enzymes. in this study, we constructed an in vitro trans-sialylation system by reconstructing the exogenous sialoglycoconjugate synthesis system of pathogens on the surfaces of yeast cells. the nanh gene en ... | 2010 | 20711574 |
| penicillium marneffei infection in an african man. | penicilliosis is a systemic fungal infection caused by penicillium marneffei. the infection is most commonly seen in southeast asia, southern china, hong kong, and taiwan. it is rarely seen among individuals of african descent. here, we report a case of penicilliosis in an african man from namibia who was studying in malaysia. he presented with multiple umbilicated papules associated with cough, fever, loss of appetite, and weight. he also had urethral discharge and admitted to unprotected sexua ... | 2010 | 20673530 |
| fission yeast telomeres forecast the end of the crisis. | recent years have placed fission yeast at the forefront of telomere research, as this organism combines a high level of conservation with human telomeres and precise genetic manipulability. here we highlight some of the latest knowledge of fission yeast telomere maintenance and dysfunction, and illustrate how principles arising from fission yeast research are raising novel questions about telomere plasticity and function in all eukaryotes. | 2010 | 20682311 |
| experimental annotation of the human pathogen candida albicans coding and noncoding transcribed regions using high-resolution tiling arrays. | compared to other model organisms and despite the clinical relevance of the pathogenic yeast candida albicans, no comprehensive analysis has been done to provide experimental support of its in silico-based genome annotation. | 2010 | 20618945 |
| the inflammatory response induced by aspartic proteases of candida albicans is independent of proteolytic activity. | the secretion of aspartic proteases (saps) has long been recognized as a virulence-associated trait of the pathogenic yeast candida albicans. in this study, we report that different recombinant saps, including sap1, sap2, sap3, and sap6, have differing abilities to induce secretion of proinflammatory cytokines by human monocytes. in particular sap1, sap2, and sap6 significantly induced interleukin-1β (il-1β), tumor necrosis factor alpha (tnf-α), and il-6 production. sap3 was able to stimulate th ... | 2010 | 20713630 |
| 14-3-3gamma mediates cdc25a proteolysis to block premature mitotic entry after dna damage. | 14-3-3 proteins control various cellular processes, including cell cycle progression and dna damage checkpoint. at the dna damage checkpoint, some subtypes of 14-3-3 (beta and zeta isoforms in mammalian cells and rad24 in fission yeast) bind to ser345-phosphorylated chk1 and promote its nuclear retention. here, we report that 14-3-3gamma forms a complex with chk1 phosphorylated at ser296, but not at atr sites (ser317 and ser345). ser296 phosphorylation is catalysed by chk1 itself after chk1 phos ... | 2010 | 20639859 |
| the migration of mitochondrial dna fragments to the nucleus affects the chronological aging process of saccharomyces cerevisiae. | migration of fragmented mitochondrial dna (mtdna) to the nucleus has been shown to occur in multiple species including yeast, plants, and mammals. several human diseases, including pallister-hall syndrome and mucolipidosis, can be initiated by mtdna insertion mutagenesis of nuclear dna. in yeast, we demonstrated that the rate of mtdna fragments translocating to the nucleus increases during chronological aging. the yeast chronological lifespan (cls) is determined by the survival of nondividing ce ... | 2010 | 20626726 |
| autophagy, proteases and the sense of balance. | the knowledge of the molecular mechanisms underlying autophagy has considerably improved after the isolation and characterization of autophagy-defective mutants in the yeast saccharomyces cerevisiae. two ubiquitin-like conjugation systems are required for yeast autophagy. one of them requires the participation of atg8 synthesized as a precursor protein, which is cleaved after a gly residue by a cysteine proteinase called atg4. the new gly-terminal residue from atg8 is activated by atg7 (an e1-li ... | 2010 | 20724821 |
| rna silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga chlamydomonas reinhardtii. | the genome of the green alga chlamydomonas reinhardtii encodes two [fefe]-hydrogenases, hyda1 and hyda2, and the hydrogenase-like protein hyd3. the unique combination of these proteins in one eukaryotic cell allows for direct comparison of their in vivo functions, which have not been established for hyda2 and hyd3. using an artificial microrna silencing method developed recently, the expression of hyda1, hyda2 and hyd3 was specifically down-regulated. silencing of hyda1 resulted in 4-fold lower ... | 2010 | 20726841 |
| learning a weighted sequence model of the nucleosome core and linker yields more accurate predictions in saccharomyces cerevisiae and homo sapiens. | dna in eukaryotes is packaged into a chromatin complex, the most basic element of which is the nucleosome. the precise positioning of the nucleosome cores allows for selective access to the dna, and the mechanisms that control this positioning are important pieces of the gene expression puzzle. we describe a large-scale nucleosome pattern that jointly characterizes the nucleosome core and the adjacent linkers and is predominantly characterized by long-range oscillations in the mono, di- and tri- ... | 2010 | 20628623 |
| targeting cell division cycle 7 kinase: a new approach for cancer therapy. | the cell division cycle 7 (cdc7) is a serine-threonine kinase, originally discovered in budding yeast, required to initiate dna replication. human cdc7 phosphorylates the minichromosome maintenance protein 2 (mcm2), a component of the dna replicative helicase needed for genome duplication. inhibition of cdc7 in cancer cells impairs progression through s phase, inducing a p53-independent apoptotic cell death, whereas in normal cells, it does not affect cell viability. small molecule compounds abl ... | 2010 | 20647475 |
| interaction of hsp40 with influenza virus m2 protein: implications for pkr signaling pathway. | influenza virus contains three integral membrane proteins: haemagglutinin, neuraminidase, and matrix protein (m1 and m2). among them, m2 protein functions as an ion channel, important for virus uncoating in endosomes of virus-infected cells and essential for virus replication. in an effort to explore potential new functions of m2 in the virus life cycle, we used yeast two-hybrid system to search for m2-associated cellular proteins. one of the positive clones was identified as human hsp40/hdj1, a ... | 2010 | 21204021 |
| [human arginase i from the recombinant yeast hansenula polymorpha: isolation and characterization of the enzyme]. | purified human arginase i preparations homogeneous in sds-paag test were obtained by the affinity chromatography on the synthesized sorbent l-arginine-macroporous glass. some physico-chemical characteristics of the isolated arginase preparation have been estimated: thermo- and ph-stability, temperature- and ph-optima of the enzyme. the influence of some bivalent metal ions and other additives on enzymatic activity for stabilization of the enzyme and optimization of its storage conditions was stu ... | 2010 | 21805858 |
| the molecular genetics of sirtuins: association with human longevity and age-related diseases. | the sirtuins are a family of proteins remarkably conserved from yeast to humans. in organisms such as yeast, worms and flies it is quite well established that the activity of sirtuins prolongs lifespan. as a result of promising findings in simple organisms, sirtuins are now investigated in higher organisms in relation to the ageing process. in mammals there are seven different sirtuin proteins each encoded by individual genes (sirt1-7). although sirtuins share a highly conserved catalytic domain ... | 2010 | 21537393 |
| the exosomes of trypanosomes and other protists. | the archaeal exosome contains three heterodimeric rnase ph subunits, forming a hexamer with rnase activity; on top sits a trimer of two different si domain proteins. in animals and yeast, six different, but related subunits form the rnase ph-like core, but these lack enzyme activity; there are three different si-domain proteins and enzyme activity is providedby the endo/exonuclease rrp44 or--mainly in the nuclear exosome--the rnase d enzyme rrp6. trypanosomes diverged from yeast and mammals very ... | 2010 | 21618873 |
| antimicrobial screening of ethnobotanically important stem bark of medicinal plants. | the stem barks are the rich sources of tannins and other phenolic compounds. tannins inhibited the growth of various fungi, yeast, bacteria and virus. hence, ten stem barks of ethnomedicinally important plants were screened for antibacterial and antifungal activities against human pathogenic strains. | 2010 | 21808577 |
| recombinant yeast technology at the cutting edge: robust tools for both designed catalysts and new biologicals. | health and safety concerns, enhanced quality criteria, and environmental sustainability, have prompted investigations into production using recombinant yeasts as a feasible alternative for isolation of proteins from natural animal or plant sources, as well as for processes utilising either mammalian cell cultures or bacterial systems. an overview of recent research papers and review articles provides readers with a comprehensive insight into the field of next-generation yeast expression systems. ... | 2010 | 21197847 |
| targeted amino-terminal acetylation of recombinant proteins in e. coli. | one major limitation in the expression of eukaryotic proteins in bacteria is an inability to post-translationally modify the expressed protein. amino-terminal acetylation is one such modification that can be essential for protein function. by co-expressing the fission yeast natb complex with the target protein in e.coli, we report a simple and widely applicable method for the expression and purification of functional n-terminally acetylated eukaryotic proteins. | 2010 | 21203426 |
| the structure of human leucine carboxyl methyltransferase 1 that regulates protein phosphatase pp2a. | leucine carboxyl methyltransferase 1 (lcmt1) methylates the terminal carboxyl group of the leucine 309 residue of human protein phosphatase 2a (pp2a). pp2a, a key regulator of many cellular processes, has recently generated additional interest as a potential cancer-therapeutic target. the status of pp2a methylation impacts upon the selection of the regulatory subunit by the pp2a core enzyme, thus directing its activity and subcellular localization. an x-ray crystal structure of human lcmt1 prote ... | 2010 | 21206058 |
| a combinatorial genetic library approach to target heterologous glycosylation enzymes to the endoplasmic reticulum or the golgi apparatus of pichia pastoris. | to humanize the glycosylation pathway in the yeast pichia pastoris, we developed several combinatorial genetic libraries and used them to properly localize active eukaryotic mannosidases and sugar transferases. here we report the details of the fusion of up to 66 n-terminal targeting sequences of fungal type ii membrane proteins to 33 catalytic domains of heterologous glycosylation enzymes. we show that while it is difficult to predict which leader/catalytic domain will result in the desired act ... | 2010 | 21213235 |
| a novel dual dbp5/ddx19 homologue from plasmodium falciparum requires q motif for activity. | helicases are ubiquitous essential enzymes which have significant role in the nucleic acid metabolism. using in silico approaches in the recent past we have identified a number of helicases in the plasmodium falciparum genome. in the present study we report purification and detailed characterization of a novel helicase from p. falciparum. our results indicate that this helicase is a homologue of dbp5 and ddx19 from yeast and human, respectively. the biochemical characterization shows that it con ... | 2010 | 21168450 |
| a novel binding protein of single immunoglobulin il-1 receptor-related molecule: paralemmin-3. | previous studies have shown that single immunoglobulin il-1 receptor-related molecule (sigirr) is a negative regulator of toll-interleukin-1 receptor signaling. nevertheless, the molecular mechanism of the negatively regulatory effect of sigirr remains unknown. using a yeast two-hybrid screen, we identified paralemmin-3 (palm3) as a novel binding protein of sigirr. this interaction of sigirr with palm3 was confirmed by coimmunoprecipitation in mammalian cells. in addition, the palm3 mrna express ... | 2010 | 21187075 |
| methylation of histone h3 lysine 36 enhances dna repair by nonhomologous end-joining. | given its significant role in the maintenance of genomic stability, histone methylation has been postulated to regulate dna repair. histone methylation mediates localization of 53bp1 to a dna double-strand break (dsb) during homologous recombination repair, but a role in dsb repair by nonhomologous end-joining (nhej) has not been defined. by screening for histone methylation after dsb induction by ionizing radiation we found that generation of dimethyl histone h3 lysine 36 (h3k36me2) was the maj ... | 2010 | 21187428 |
| disruption of a sirt1-dependent autophagy checkpoint in the prostate results in prostatic intraepithelial neoplasia lesion formation. | the sirtuin family of proteins (sirt) encode a group of evolutionarily conserved, nad-dependent histone deacetylases, involved in many biological pathways. sirt1, the human homologue of the yeast silent information regulator 2 (sir2) gene, deacetylates histones, p300, p53, and the androgen receptor. autophagy is required for the degradation of damaged organelles and long-lived proteins, as well as for the development of glands such as the breast and prostate. herein, homozygous deletion of the s ... | 2010 | 21189328 |
| environments that induce synthetic microbial ecosystems. | interactions between microbial species are sometimes mediated by the exchange of small molecules, secreted by one species and metabolized by another. both one-way (commensal) and two-way (mutualistic) interactions may contribute to complex networks of interdependencies. understanding these interactions constitutes an open challenge in microbial ecology, with applications ranging from the human microbiome to environmental sustainability. in parallel to natural communities, it is possible to explo ... | 2010 | 21124952 |
| the hpv16 e6 binding protein tip-1 interacts with arhgef16, which activates cdc42. | guanidine exchange factor (gef)-catalysed activation of rho proteins such as cdc42 has been shown to have a crucial role in cellular transformation, malignant progression and invasion. we have previously shown that the hpv16 e6 oncoprotein binds to the pdz domain protein tax-interacting-protein 1 (tip-1) and we now report identification and functional analysis of a novel tip-1 binding gef. | 2010 | 21139582 |
| a set of aspartyl protease-deficient strains for improved expression of heterologous proteins in kluyveromyces lactis. | secretion of recombinant proteins is a common strategy for heterologous protein expression using the yeast kluyveromyces lactis. however, a common problem is degradation of a target recombinant protein by secretory pathway aspartyl proteases. in this study, we identified five putative pfam00026 aspartyl proteases encoded by the k. lactis genome. a set of selectable marker-free protease deletion mutants was constructed in the prototrophic k. lactis gg799 industrial expression strain background us ... | 2010 | 21166768 |
| suicidal gene therapy in an nf-κb-controlled tumor environment as monitored by a secreted blood reporter. | the nuclear factor-κb (nf-κb) is known to be activated in many cancer types including lung, ovarian, astrocytomas, melanoma, prostate as well as glioblastoma, and has been shown to correlate with disease progression. we have cloned a novel nf-κb-based reporter system (five tandem repeats of nf-κb responsive genomic element (nf; 14 bp each)) to drive the expression cassette for both a fusion between the yeast cytosine deaminase and uracil phosphoribosyltransferase (cu) as a therapeutic gene and t ... | 2010 | 21150937 |
| mixed hsp90-cochaperone complexes are important for the progression of the reaction cycle. | the chaperone cycle of heat shock protein-90 (hsp90) involves progression through defined complexes with different cochaperones. it is still enigmatic how the exchange of cochaperones is regulated. the first cochaperone entering the cycle is the hsp90 atpase inhibitor sti1 (hop in human), which later is replaced by a prolyl isomerase (ppiase) and p23. we found, unexpectedly, that one sti1 molecule is sufficient to completely inhibit the atpase of the hsp90 dimer. upon addition of a ppiase cochap ... | 2010 | 21170051 |
| effect of tin and lead chlorotriphenyl analogues on selected living cells. | three kinds of living cells, human embryonic kidney cells, saccharomyces cerevisiae, and escherichia coli, were tested for their sensitivity to chlorotriphenyltin and chlorotriphenyllead. the tin compound proved definitely more toxic than the lead derivative, particularly in the case of the human embryonic kidney cells devoid of any protective cell wall. electron paramagnetic resonance (epr) comparative studies carried out by using a natural model liposome system (egg yolk lecithin) confirmed co ... | 2010 | 21154756 |
| identification and engineering of human variable regions that allow expression of stable single-chain t cell receptors. | single-chain antibody fragments (scfv), consisting of two linked variable regions (v(h) and v(l)), are a versatile format for engineering and as potential antigen-specific therapeutics. although the analogous format for t cell receptors (tcrs), consisting of two linked v regions (vα and vβ; referred to here as sctv), could provide similar opportunities, all wild-type sctv proteins examined to date are unstable. this obstacle has prevented sctv fragments from being widely used for engineering or ... | 2010 | 21159619 |
| activation of amyloid precursor protein processing by growth factors is dependent on ras gtpase activity. | the β-amyloid peptide is generated by the proteolysis of the amyloid precursor protein (app) by the action of β- and γ-secretase. the mechanisms underlying this process are poorly understood. using a cell-based reporter gene assay we analysed the possible signals and pathways that could be involved in app cleavage. we used the stable cell line hela ag that expresses the human app(695) fused with the yeast transcription factor gal4. this fusion protein is normally translocated into the plasma mem ... | 2010 | 21161594 |
| generation of rna/dna hybrids in genomic dna by transformation using rna-containing oligonucleotides. | synthetic short nucleic acid polymers, oligonucleotides (oligos), are the most functional and widespread tools of molecular biology. oligos can be produced to contain any desired dna or rna sequence and can be prepared to include a wide variety of base and sugar modifications. moreover, oligos can be designed to mimic specific nucleic acid alterations and thus, can serve as important tools to investigate effects of dna damage and mechanisms of repair. we found that thermo scientific dharmacon rn ... | 2010 | 21178953 |
| addressing trypsin bias in large scale (phospho)proteome analysis by size exclusion chromatography and secondary digestion of large post-trypsin peptides. | in the vast majority of bottom-up proteomics studies, protein digestion is performed using only mammalian trypsin. although it is clearly the best enzyme available, the sole use of trypsin rarely leads to complete sequence coverage, even for abundant proteins. it is commonly assumed that this is because many tryptic peptides are either too short or too long to be identified by rplc-ms/ms. we show through in silico analysis that 20-30% of the total sequence of three proteomes (schizosaccharomyces ... | 2010 | 21166477 |
| functional null mutations of msrb3 encoding methionine sulfoxide reductase are associated with human deafness dfnb74. | the dfnb74 locus for autosomal-recessive, nonsyndromic deafness segregating in three families was previously mapped to a 5.36 mb interval on chromosome 12q14.2-q15. subsequently, we ascertained five additional consanguineous families in which deafness segregated with markers at this locus and refined the critical interval to 2.31 mb. we then sequenced the protein-coding exons of 18 genes in this interval. the affected individuals of six apparently unrelated families were homozygous for the same ... | 2010 | 21185009 |
| crystal structure of uba2(ufd)-ubc9: insights into e1-e2 interactions in sumo pathways. | canonical ubiquitin-like proteins (ubls) such as ubiquitin, sumo, nedd8, and isg15 are ligated to targets by e1-e2-e3 multienzyme cascades. the sumo cascade, conserved among all eukaryotes, regulates numerous biological processes including protein localization, transcription, dna replication, and mitosis. sumo conjugation is initiated by the heterodimeric aos1-uba2 e1 enzyme (in humans called sae1-uba2), which activates sumo's c-terminus, binds the dedicated e2 enzyme ubc9, and promotes sumo c-t ... | 2010 | 21209884 |
| mutations causing greenberg dysplasia but not pelger anomaly uncouple enzymatic from structural functions of a nuclear membrane protein. | the lamin b receptor (lbr) is an inner nuclear membrane protein with a structural function interacting with chromatin and lamins, and an enzymatic function as a sterol reductase. heterozygous lbr mutations cause nuclear hyposegmentation in neutrophils (pelger anomaly), while homozygous mutations cause prenatal death with skeletal defects and abnormal sterol metabolism (greenberg dysplasia). it has remained unclear whether the lethality in greenberg dysplasia is due to cholesterol defects or alte ... | 2010 | 21327084 |
| blastomycosis presenting as multiple splenic abscesses: case report and review of the literature. | a 31-year-old canadian aboriginal man from northwestern ontario presented with left upper quadrant pain and a tender left upper quadrant mass. evaluation with a computed tomography scan showed multiple lesions within the spleen, a collection between the splenic tip and splenic flexure of the colon, and several small adrenal lesions. computed tomographic-guided needle biopsy showed necrotizing granulomatous inflammation and multinucleated giant cells. gomori's methenamine silver stain showed broa ... | 2010 | 21358886 |
| [development of a yeast two-hybrid screen for selection of a/h1n1 influenza ns1 non-structural protein and human cpsf30 protein interaction inhibitors]. | influenza a/h1n1 virus-encoded nonstructural, or ns1, protein inhibits the 3'-end processing of cellular pre-mrnas by binding the cellular protein: the 30-kda subunit of cpsf (cleavage and polyadenylation specificity factor, cpsf30). cpsf30 binding site of the ns1 protein is a potential target for the development of drugs against influenza a/h1n1 virus. a yeast two-hybrid screening system was constructed and used for screening chinese medicines that inhibit the interaction of the a/h1n1 flu ns1 ... | 2010 | 21351518 |
| vital function of preli and essential requirement of its lea motif. | proteins containing the late embryogenesis abundant (lea) motif comprise a conserved family, postulated to act as cell protectors. however, their function and mechanisms of action remain unclear. here we show that preli, a mammalian lea-containing homolog of yeast ups1p, can associate with dynamin-like gtpase optic atrophy-1 (opa1) and contribute to the maintenance of mitochondrial morphology. accordingly, preli can uphold mitochondrial membrane potential (δψ(m)) and enhance respiratory chain (r ... | 2010 | 21364629 |
| upregulated expression of hitf in crohn's disease and screening of hitf interactant by a yeast two-hybrid system. | to study the expression of human intestinal trefoil factor (hitf) mrna in crohn's disease and to screen the cellular proteins that can interact with the hitf protein by a yeast two-hybrid system in order to explore the mechanism of hitf in protecting intestinal mucosa from injury. | 2010 | 20499178 |
| soluble cytoplasmic expression, rapid purification, and characterization of cyanovirin-n as a his-sumo fusion. | cyanovirin-n (cvn) is a promising antiviral candidate that has an extremely low sequence homology with any other known proteins. the efficient and soluble expression of biologically functional recombinant cvn (rcvn) is still an obstacle due to insufficient yield, aggregation, and abnormal modification. here, we describe an improved approach to preparing native rcvn from escherichia coli more efficiently. a fusion gene consisting of cvn and sumo (small ubiquitin-related modifier) and a hexahistid ... | 2010 | 19547966 |