Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| stereo- and region-specific biotransformation of physapubescin by four fungal strains. | biotransformations of physapubescin (1) were performed by four fungal strains-mucor subtilissimus as 3.2454, mucor polymorphosporus as 3.3443, aspergillus niger as 3.795, and syncephalastrum racemosum as 3.264. four metabolites were prepared in the biotransformation process of 1, and their structures were elucidated as 15α-acetoxy-5,6β:22,26:24,25-triepoxy-26α-hydroxy-3β-methoxy 4β-hydroxyergost-1-one (2), 15α-acetoxy-5,6β:22,26-diepoxy-4β,24β,25α,26(α, β)-tetrahydroxyergost-3β-methoxy-1-one (3a ... | 2017 | 28074432 |
| enantioselective resolution of (r,s)-carvedilol to (s)-(-)-carvedilol by biocatalysts. | among the microorganisms employed in the study, aspergillus niger (gufcc5443), escherichia coli (atcc9637), streptomyces halstedii (ckm-2), pseudomonas putida (ncib9494), cunninghamella elegans (ncim689) and sphingomonas paucimobilis (nctc11030) were capable for the enantioselective conversion of racemic carvedilol. immobilization technique enhanced the enantioselectivity of microorganisms and thus increased the enantiomeric purity of the drug. excellent enantiomeric ratios (e) were found in rea ... | 2017 | 28064425 |
| biogeochemical spatio-temporal transformation of copper in aspergillus niger colonies grown on malachite with different inorganic nitrogen sources. | this work elucidates spatio-temporal aspects of the biogeochemical transformation of copper mobilized from malachite (cu2 (co3 )(oh)2 ) and bioaccumulated within aspergillus niger colonies when grown on different inorganic nitrogen sources. it was shown that the use of either ammonium or nitrate determined how copper was distributed within the colony and its microenvironment and the copper oxidation state and succession of copper coordinating ligands within the biomass. nitrate-grown colonies yi ... | 2017 | 28063182 |
| evaluation of wound healing, anti-microbial and antioxidant potential of pongamia pinnata in wistar rats. | to investigate wound healing, antimicrobial and antioxidant activity of leaf extract of pongamia pinnata. | 2017 | 28053891 |
| improvement of glucoamylase production for raw-starch digestion in aspergillus niger f-01 by maltose stearic acid ester. | to investigate the role of maltose stearic acid ester (msae) for improving raw-starch-digesting glucoamylase (rsdg) production by aspergillus niger f-01 and its regulatory mechanism. | 2017 | 28044224 |
| antifungal effect of malaysian aloe vera leaf extract on selected fungal species of pathogenic otomycosis species in in vitro culture medium. | aloe barbadensis miller or aloe vera has been used for therapeutic purposes since ancient times with antifungal activity known to be amongst its medicinal properties. we conducted a pilot study to determine the antifungal properties of malaysian aloe vera leaf extract on otomycosis species including aspergillus niger and candida albicans. | 2017 | 28042402 |
| design and composition of synthetic fungal-bacterial microbial consortia that improve lignocellulolytic enzyme activity. | microbial interactions are important for metabolism as they can improve or reduce metabolic efficiency. to improve lignocellulolytic enzyme activity, a series of synergistic microbial consortia of increasing diversity and complexity were devised using fungal strains, including trichoderma reesei, penicillium decumbens, aspergillus tubingensis, and aspergillus niger. however, when a screened microbial community with cellulolytic capacity was added to the consortia to increase the number of strain ... | 2017 | 28039824 |
| physicochemical and antifungal properties of bio-nanocomposite film based on gelatin-chitin nanoparticles. | the gelatin-based nanocomposite films containing chitin nanoparticles (n-chitin) with concentrations of 0, 3, 5 and 10% were prepared and their physical, thermal and anti-microbial properties were investigated. scanning electron microscopy (sem) micrographs showed that n-chitin size distribution was around 60-70nm which dispersed appropriately at low concentration in gelatin matrix. the results showed that incorporation of n-chitin significantly influenced apparent color and transparency of the ... | 2017 | 28034825 |
| establishment of the inducible tet-on system for the activation of the silent trichosetin gene cluster in fusarium fujikuroi. | the pks-nrps-derived tetramic acid equisetin and its n-desmethyl derivative trichosetin exhibit remarkable biological activities against a variety of organisms, including plants and bacteria, e.g., staphylococcus aureus. the equisetin biosynthetic gene cluster was first described in fusarium heterosporum, a species distantly related to the notorious rice pathogen fusarium fujikuroi. here we present the activation and characterization of a homologous, but silent, gene cluster in f. fujikuroi. bio ... | 2017 | 28379186 |
| the dynamic microbiota profile during pepper (piper nigrum l.) peeling by solid-state fermentation. | white pepper (piper nigrum l.), a well-known spice, is the main pepper processing product in hainan province, china. the solid-state method of fermentation can peel pepper in a highly efficient manner and yield high-quality white pepper. in the present study, we used next-generation sequencing to reveal the dynamic changes in the microbiota during pepper peeling by solid-state fermentation. the results suggested that the inoculated aspergillus niger was dominant throughout the fermentation stage ... | 2017 | 28378160 |
| synthesis and structure-activity relationships of novel benzylamine-type antifungals as butenafine-related antimycotics. | benzylamine-type antimycotics like naftifine, butenafine, or terbinafine are a well-known class of antimycotics since the 1980s. the following paper describes the synthesis and biological evaluation of a series of novel benzylamine-type antimycotics characterized by an isooctyl side chain and various substituents at the benzylamine moiety. the compounds were prepared from benzaldehyde derivatives and 2-amino-6-methylheptane by reductive amination with sodium triacetoxyborohydride and subsequent ... | 2017 | 28376264 |
| thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production. | nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at brazilian cerrado and identified up to species level on the basis of the gene sequencing of 5.8s-its and 26s ribosomal dna regions. four species were identified: kluyveromyces marxianus, aspergillus niger, aspergillus sydowii and aspergillus fumigatus, and the isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. among them, th ... | 2017 | 26413077 |
| elucidation of biosynthetic pathways of natural products. | during the last decade, we have revealed biosynthetic pathways responsible for the formation of important and chemically complex natural products isolated from various organisms through genetic manipulation. detailed in vivo and in vitro characterizations enabled elucidation of unexpected mechanisms of secondary metabolite biosynthesis. this personal account focuses on our recent efforts in identifying the genes responsible for the biosynthesis of spirotryprostatin, aspoquinolone, sch 210972, py ... | 2017 | 28387469 |
| an evolutionarily conserved transcriptional activator-repressor module controls expression of genes for d-galacturonic acid utilization in aspergillus niger. | the expression of genes encoding extracellular polymer-degrading enzymes and the metabolic pathways required for carbon utilization in fungi are tightly controlled. the control is mediated by transcription factors that are activated by the presence of specific inducers, which are often monomers or monomeric derivatives of the polymers. a d-galacturonic acid-specific transcription factor named gaar was recently identified and shown to be an activator for the expression of genes involved in galact ... | 2017 | 28049705 |
| antifungal activity of lactobacillus against microsporum canis, microsporum gypseum and epidermophyton floccosum. | a total of 220 lactic acid bacteria isolates were screened for antifungal activity using aspergillus fumigatus and aspergillus niger as the target strains. four lactobacillus strains exhibited strong inhibitory activity on agar surfaces. all four were also identified as having strong inhibitory activity against the human pathogenic fungi microsporum canis, microsporum gypseum and epidermophyton floccosum. one of the four lactobacilli, namely lb. reuteri ee1p exhibited the most inhibition against ... | 2017 | 22539027 |
| quantifying the effect of water activity and storage temperature on single spore lag times of three moulds isolated from spoiled bakery products. | the inhibitory effect of water activity (aw) and storage temperature on single spore lag times of aspergillus niger, eurotium repens (aspergillus pseudoglaucus) and penicillium corylophilum strains isolated from spoiled bakery products, was quantified. a full factorial design was set up for each strain. data were collected at levels of aw varying from 0.80 to 0.98 and temperature from 15 to 35°c. experiments were performed on malt agar, at ph5.5. when growth was observed, ca 20 individual growth ... | 2017 | 27325576 |
| simultaneous production of amyloglucosidase and exo-polygalacturonase by aspergillus niger in a rotating drum reactor. | simultaneous production of amyloglucosidase (amg) and exo-polygalacturonase (exo-pg) was carried out by aspergillus niger in substrate of defatted rice bran in a rotating drum bioreactor (rdb) and studied by a 3(1) × 2(2) factorial experimental design. variables under study were a. niger strains (a. niger nrrl 3122 and a. niger t0005/007-2), types of inoculum (spore suspension and fermented bran), and types of inducer (starch, pectin, and a mix of both). solid-state fermentation process (ssf) wa ... | 2017 | 27704476 |
| comprehensive reconstruction and in silico analysis of aspergillus niger genome-scale metabolic network model that accounts for 1210 orfs. | aspergillus niger is one of the most important cell factories for industrial enzymes and organic acids production. a comprehensive genome-scale metabolic network model (gsmm) with high quality is crucial for efficient strain improvement and process optimization. the lack of accurate reaction equations and gene-protein-reaction associations (gprs) in the current best model of a. niger named gsmm ima871, however, limits its application scope. to overcome these limitations, we updated the a. niger ... | 2017 | 27696371 |
| effect of passive transport of water through plasma membrane in production of extracellular enzyme. | in this article, availability and control of water in solid-state fermentation (ssf) were investigated. based on passive transport of water through plasma membranes, a new model was proposed for calculation and control of water activities in the mixture of solids. the validity of theoretical model and accuracy of the proposed model were proved by experimental data. this model was used for production of pectinases via mixed-ssf with the aid of a rotary drum bioreactor. it was found that in case o ... | 2017 | 27778113 |
| ultrasound-assisted hydrolysis of waste cooking oil catalyzed by homemade lipases. | this study aimed to evaluate the waste cooking oil (wco) hydrolysis in ultrasonic system using lipase as catalyst. lipase was produced by the fungus aspergillus niger via solid state fermentation (ssf) using canola meal as substrate. prior to the hydrolysis reaction, the lipase behavior when subjected to ultrasound was evaluated by varying the temperature of the ultrasonic bath, the exposure time and the equipment power. having optimized the treatment on ultrasound, the wco hydrolysis reaction w ... | 2017 | 27746067 |
| active edible coating and γ-irradiation as cold combined treatments to assure the safety of broccoli florets (brassica oleracea l.). | the antimicrobial activity of essential oils (eos), organic acid (oa) salts and natamycin, a natural antifungal produced during fermentation by the bacterium streptomyces natalensis, was assessed against four pathogens (escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium and aspergillus niger). the minimum inhibitory concentration (mic) of each antimicrobial (am) was assessed to determine their efficiency on tested microbial species in order to select the most efficient. the ... | 2017 | 27744210 |
| effects of biopellets composed of microalgae and fungi on cadmium present at environmentally relevant levels in water. | removal of cadmium (cd) present at low levels (1 µg l(-1)) in water was investigated using three different microorganism treatments: the microalga chlorella vulgaris, the fungus aspergillus niger, and for the first time biopellets composed of c. vulgaris and a. niger. after 5 days, all microorganism treatments resulted in significantly lower cd concentrations compared with the control. biopellets treatment resulted in significantly lower ph values than other microorganism treatments, indicating ... | 2017 | 27739868 |
| influence of physical and chemical characteristics of wine grapes on the incidence of penicillium and aspergillus fungi in grapes and ochratoxin a in wines. | the incidence of filamentous fungi and toxin levels in grapes and wines varies depending on the variety of grapes, the wine region, agricultural practices, weather conditions, the harvest and the winemaking process. in this sense, the objective of this study was to evaluate the diversity of aspergillus and penicillium fungi isolated from wine grapes of the semi-arid tropical region of brazil, evaluate the presence of ochratoxin a (ota) in the experimental wine and verify if there is a correlatio ... | 2017 | 27792949 |
| enhanced recovery of valuable metals from spent lithium-ion batteries through optimization of organic acids produced by aspergillus niger. | in the present study, spent medium bioleaching method was performed using organic acids produced by aspergillus niger to dissolve ni, co, mn, li, cu and al from spent lithium-ion batteries (libs). response surface methodology was used to investigate the effects and interactions between the effective factors of sucrose concentration, initial ph, and inoculum size to optimize organic acid production. maximum citric acid, malic acid, and gluconic acid concentrations of 26,478, 1832.53 and 8433.76pp ... | 2017 | 27825532 |
| selective ultrasound-enhanced enzymatic hydrolysis of oleuropein to its aglycon in olive (olea europaea l.) leaf extracts. | hydrolysis of oleuropein, the main phenol in olive (olea europaea l.) leaf extracts, to oleuropein aglycon and other subsequent products in the hydrolytic pathway can be catalyzed by different enzymes. three of the most used hydrolases were assayed to catalyze the process, and β-glucosidase from aspergillus niger was selected. acceleration of the enzymatic hydrolysis by ultrasound (us) was studied using a box-behnken design (duty cycle, amplitude, cycle time) and an oleuropein standard, and the ... | 2017 | 27855900 |
| mycotoxins: a fungal genomics perspective. | the chemical and enzymatic diversity in the fungal kingdom is staggering. large-scale fungal genome sequencing projects are generating a massive catalog of secondary metabolite biosynthetic genes and pathways. fungal natural products are a boon and bane to man as valuable pharmaceuticals and harmful toxins. understanding how these chemicals are synthesized will aid the development of new strategies to limit mycotoxin contamination of food and feeds as well as expand drug discovery programs. a su ... | 2017 | 27924551 |
| immobilization of indigenous holocellulase on iron oxide (fe2o3) nanoparticles enhanced hydrolysis of alkali pretreated paddy straw. | the holocellulase from aspergillus niger sh3 was characterized and found to contain 125 proteins including cellulases (26), hemicellulases (21), chitinases (10), esterases (6), amylases (4) and hypothetical protein (32). the crude enzyme was immobilized on five different nanoparticles (nps) via physical adsorption and covalent coupling methods. the enzyme-nanoparticle complexes (enc) were screened for protein binding, enzymatic activities and immobilization efficiency. magnetic enzyme-nanopartic ... | 2017 | 27919810 |
| fermentative production of high titer citric acid from corn stover feedstock after dry dilute acid pretreatment and biodetoxification. | the aim of this work is to study the citric acid fermentation by a robust strain aspergillus niger siim m288 using corn stover feedstock after dry dilute sulfuric acid pretreatment and biodetoxification. citric acid at 100.04g/l with the yield of 94.11% was obtained, which are comparable to the starch or sucrose based citric acid fermentation. no free wastewater was generated in the overall process from the pretreatment to citric acid fermentation. abundant divalent metal ions as well as high ti ... | 2017 | 27913168 |
| biochemical and genetic basis of orsellinic acid biosynthesis and prenylation in a stereaceous basidiomycete. | the prenylphenols are a class of natural products that have been frequently isolated from basidiomycetes, e.g., from the genus stereum (false turkey tail fungi) and other russulales as well as from ascomycetes. biosynthetically, these compounds are considered hybrids, as the orsellinic acid moiety is a polyketide and the prenyl side chain originates from the terpene metabolism, although no literature on the genetic and biochemical background of the biosynthesis is available. in a stereaceous bas ... | 2017 | 27903443 |
| synthesis and antimicrobial evaluation of ester-linked 1,4-disubstituted 1,2,3-triazoles with a furyl/thienyl moiety. | twenty ester-linked 1,4-disubstituted 1,2,3-triazoles having a furyl/thienyl moiety have been synthesized from heteroaryl prop-2-yn-1-yl carboxylate and aromatic azides via a cu(i) catalyzed 1,3-dipolar cycloaddition. all the synthesized compounds were characterized by ftir, [formula: see text]h nmr, [formula: see text]c nmr spectroscopy and hrms. synthesized triazoles were tested in vitro for antimicrobial evaluation against gram-negative bacteria-escherichia coli, enterobacter aerogenes and kl ... | 2017 | 27900513 |
| a possible water-soluble inducer for synthesis of cellulase in aspergillus niger. | the synthesis of cellulase in filamentous fungi can be triggered by several inducers. in this study, a bamboo-shoot shell pretreated with pleurotus ostreatus could promote the formation of cellulases in aspergillus niger. further identification, including uplc-tof-ms, ultrafiltration, and ft-ir, denoted that the soluble inducer was not a traditional disaccharide but a type of modified lignin polymer. this revelation may result in incipient strategies to ameliorate cellulase productivity. | 2017 | 27993445 |
| preparation and characterization of intelligent starch/pva films for simultaneous colorimetric indication and antimicrobial activity for food packaging applications. | we have developed an intelligent starch/poly-vinyl alcohol (pva) film that is capable of monitoring ph changes and inhibiting undesired microbial growth in foods. starch and pva polymers in the film were doubly cross-linked by sodium trimetaphosphate and boric acid to improve their water-resistance and mechanical strength. anthocyanins (ant) and limonene (lim) were used to achieve simultaneous colorimetric indication and antimicrobial activity. firstly, the characterization of surface morphology ... | 2017 | 27987998 |
| maturation of conidia on conidiophores of aspergillus niger. | conidia of aspergillus niger are produced on conidiophores. here, maturation of conidia on these asexual reproductive structures was studied. pigmented conidia that had developed on conidiophores for 2, 5, and 8days were similarly resistant to heat and were metabolically active as shown by co2 release and conversion of the metabolic probe tempone. a total number of 645-2421 genes showed a ⩾2-fold change in expression when 2-day-old conidia were compared to 5- and 8-day-old spores. melanin was ex ... | 2017 | 28011318 |
| antimicrobial assay and genetic screening of selected freshwater cyanobacteria and identification of a biomolecule dihydro-2h-pyran-2-one derivative. | explorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and methicillin-resistant staphylococcus aureus (mrsa) strains) drug resource using bioassay, nrps (non-ribosomal polypeptide synthetase) and pks (polyketide synthase) genes, as well as in silico approach. | 2017 | 28004519 |
| identification and genomic analysis of antifungal property of a tomato root endophyte pseudomonas sp. p21. | pseudomonas sp., which occupy a variety of ecological niches, have been widely studied for their versatile metabolic capacity to promote plant growth, suppress microbial pathogens, and induce systemic resistance in plants. in this study, a pseudomonas sp. strain p21, which was isolated from tomato root endophytes, was identified as having antagonism against aspergillus niger. further analysis showed that this strain had the ability to biosynthesise siderophores and was less effective in inhibiti ... | 2017 | 28000056 |
| structural, textural and morphological characteristics of tannins from acacia mearnsii encapsulated using sol-gel methods: applications as antimicrobial agents. | tannins from acacia mearnsii were encapsulated using four different sol-gel methods acid (sgar), basic (sgbr), silicate (sgsr) and non-hydrolytic (sgnhr) routes. the hybrid materials were analyzed using a set of techniques to characterize their structure, texture and morphology. the antimicrobial performance of the encapsulated materials was evaluated against different microorganisms (staphylococcus aureus, escherichia coli, aspergillus niger and candida sp.). the data showed that the encapsulat ... | 2017 | 27940166 |
| effects of β-caryophyllene and murraya paniculata essential oil in the murine hepatoma cells and in the bacteria and fungi 24-h time-kill curve studies. | orange jessamine [murraya paniculata l. (rutaceae)] has been used worldwide in folk medicine as an anti-inflammatory, antibiotic and analgesic. | 2017 | 27927082 |
| the graphene oxide and chitosan biopolymer loads tio2 for antibacterial and preservative research. | microorganism breeding is a known cause of food spoilage and disease transmission. aspergillus niger (a. niger) and bacillus subtilis (b. subtilis) are examples of microorganisms that cause deterioration of fresh fruits and vegetables during storage, which can be a serious threat to human health. in this work, we synthesized a self-assembled film of graphene oxide (go) and chitosan (cs) biopolymers with titanium dioxide (tio2) nanoparticles embedded in its surface. we then characterized its anti ... | 2017 | 27979202 |
| identification and safety evaluation of a product from the biodegradation of ochratoxin a by an aspergillus strain. | ochratoxin a (ota) poses a serious health hazard throughout the world and is often reported in food commodities. at present, biological approaches to detoxifying ota are now widely considered to be the most promising methods. | 2017 | 27059118 |
| pellet-dispersion strategy to simplify the seed cultivation of aspergillus niger and optimize citric acid production. | citric acid (ca) as an extremely important platform compound has attracted intense attention due to wide applications and huge markets. here, we proposed a novel method, using pellet inoculation to replace spores, and constructed the seed recycling cultivation process, effectively avoided the longtime (spore preparation 30 days) of seed culture (including spores germination 12 h) in the traditional batch-fermentation. on this basis, using pellet-dispersion strategy, the bottleneck caused by the ... | 2017 | 27573803 |
| variability among strains of aspergillus section nigri with capacity to degrade tannic acid isolated from extreme environments. | tannins are polyphenolic compounds that cause astringent flavor and turbidity in food. tannase is an enzyme that catalyzes the hydrolysis of tannins and is used in food industry. this study was conducted to determine the genetic variability and the tannase alleles variation in fungal strains isolated from soil and plants at five extreme areas of coahuila, méxico. two screening assays under 1 and 20 % of tannic acid were performed, with the isolations. in these assays, it was possible to identify ... | 2017 | 27535833 |
| chemical composition and biological activities of the essential oil from rubus pungens var. oldhamii. | this paper presents a study on chemical composition, antimicrobial, antioxidant and tyrosinase inhibitory properties of the essential oil from leaves of rubus pungens var. oldhamii (reo). the major component of the reo is sesquiterpenes (36.04%), which consists of 1,5-cyclooctadiene,3-(1-me thylallyl)-(8ci)(17.66%), 5,6-diethenyl-1-methylcyclohexene (12%), (+) - γ-elemene (10.48%) and β-caryophyllene (8.39%).the reo is shown to be moderately active against staphylococcus aureus, aspergillus nige ... | 2017 | 27817203 |
| amphotericin b-copper(ii) complex shows improved therapeutic index in vitro. | the amb-cu(ii) complex has recently been reported as an antifungal agent with reduced aggregation of amb in aqueous solutions, increased anti c. albicans activity and lower toxicity against human cells in vitro. in the present work, investigations of the activity of the amb-cu (ii) complex against fungal pathogens with varying susceptibility, including c. albicans and c. parapsilosis strains and intrinsically resistant a. niger, and cytotoxicity in normal human dermal fibroblasts (nhdf) in vitro ... | 2017 | 27816628 |
| aspergillus niger - a possible new etiopathogenic agent in tinea capitis? presentation of two cases. | tinea capitis is generally considered as the most frequent fungal infection in childhood, as it accounts for approximately 92% of all mycosis in children. the epidemiology of this disease varies widely ranging from antropophillic, zoophilic, and geophillic dermatophytes, as the main causative agent in different geographic areas, depending on several additional factors. nowadays, the etiology is considered to vary with age, as well with gender, and general health condition. the former reported ex ... | 2017 | 26963152 |
| pathway transfer in fungi. | itaconic acid is an important building block for the chemical industry. currently, aspergillus terreus is the main organism used for itaconic acid production. due to the enormous citric acid production capacity of aspergillus niger, this host is investigated as a potential itaconic acid production host. several strategies have been tried so far: fermentation optimization, expression of cis-aconitate decarboxylase (cada) alone and in combination with aconitase targeted to the same compartment, ch ... | 2017 | 25482236 |
| glucoamylase: a current allergen in the baking industry. | over a 10 year period a decline in the rate of sensitizations to α-amylase (aspergillus oryzae) was observed in bakers investigated for allergic obstructive airway disease. at the same time, glucoamylase (aspergillus niger) was identified as the currently the most relevant allergen in sensitizations to enzymes in the baking industry. the aim of the present study was to investigate whether, over a period of 10 years and in the case of new-onset disease, there had been any change in sensitization ... | 2017 | 26120537 |
| assessment of multi-contaminant exposure in a cancer treatment center: a 2-year monitoring of molds, mycotoxins, endotoxins, and glucans in bioaerosols. | indoor air quality in health care facilities is a major public health concern, particularly for immunocompromised patients who may be exposed to microbiological contaminants such as molds, mycotoxins, endotoxins, and (1,3)-ß-d-glucans. over 2 years, bioaerosols were collected on a monthly basis in a cancer treatment center (centre f. baclesse, normandy, france), characterized from areas where there was no any particular air treatment. results showed the complexity of mycoflora in bioaerosols wit ... | 2017 | 28012082 |
| multicenter study of method-dependent epidemiological cutoff values for detection of resistance in candida spp. and aspergillus spp. to amphotericin b and echinocandins for the etest agar diffusion method. | method-dependent etest epidemiological cutoff values (ecvs) are not available for susceptibility testing of either candida or aspergillus species with amphotericin b or echinocandins. in addition, reference caspofungin mics for candida spp. are unreliable. candida and aspergillus species wild-type (wt) etest mic distributions (microorganisms in a species-drug combination with no detectable phenotypic resistance) were established for 4,341 candida albicans, 113 c. dubliniensis, 1,683 c. glabrata ... | 2017 | 27799206 |
| the nondermatophyte molds: emerging as leading cause of onychomycosis in south-east rajasthan. | onychomycosis is a fungal disease of the nail apparatus caused by both dermatophytic and nondermatophytic strains. treatment involves long duration antifungal therapy. however, long treatment duration without identifying the causative species may lead to resistance. confirmation of diagnosis and speciation by culture before administering antifungal therapy is ideal. | 2017 | 25821729 |
| lactobacillus plantarum with broad antifungal activity: a promising approach to increase safety and shelf-life of cereal-based products. | cereal-based fermented products are worldwide diffused staple food resources and cereal-based beverages represent a promising innovative field in the food market. contamination and development of spoilage filamentous fungi can result in loss of cereal-based food products and it is a critical safety concern due to their potential ability to produce mycotoxins. lactic acid bacteria (lab) have been proposed as green strategy for the control of the moulds in the food industry due to their ability to ... | 2017 | 27240933 |
| surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi. | a plackett-burman factorial design of 16 experiments was conducted to assess the influence of nine factors on the production of lipases by filamentous fungi. the factors investigated were bran type (used as the main carbon source), nitrogen source, nitrogen source concentration, inducer, inducer concentration, fungal strain (aspergillus niger or aspergillus flavus were selected as good lipase producers via submerged fermentation), ph and agitation. the concentration of the yeast extract and soyb ... | 2017 | 26991270 |
| multiplex detection of aspergillus species. | multiplex real-time polymerase chain reaction (pcr) provides a fast and accurate dna-based tool for the simultaneous amplification of more than one target sequence in a single reaction. here a duplex real-time pcr assay is described for the simultaneous detection of aspergillus carbonarius and members of the aspergillus niger aggregate, which are the main responsible species for ochratoxin a (ota) contamination in grapes. this single tube reaction targets the beta-ketosynthase and the acyl trans ... | 2017 | 27924544 |
| chemical composition and antifungal activity of essential oils from medicinal plants of kazakhstan. | the composition of essential oils from leaves of kazakhstan medicinal plants was analysed by gc-ms. the major compounds identified were 1,8-cineole (34.2%), myrcene (19.1%) and α-pinene (9.4%) in ajania fruticulosa; 1,8-cineole (21.0%), β-thujone (11.0%), camphor (8.5%), borneol (7.3%) and α-thujone (6.5%), in achillea nobilis; camphor (47.3%), 1,8-cineole (23.9%), camphene (9.8%) and β-thujone (6.0%) in artemisia terrae-albae; 1,8-cineole(55.8%) and β-pinene (6.2%) in hyssopus ambiguus; α-thuye ... | 2017 | 27879146 |
| novel fungal fad glucose dehydrogenase derived from aspergillus niger for glucose enzyme sensor strips. | in this study, a novel fungus fad dependent glucose dehydrogenase, derived from aspergillus niger (angdh), was characterized. this enzyme's potential for the use as the enzyme for blood glucose monitor enzyme sensor strips was evaluated, especially by investigating the effect of the presence of xylose during glucose measurements. the substrate specificity of angdh towards glucose was investigated, and only xylose was found as a competing substrate. the specific catalytic efficiency for xylose co ... | 2017 | 27573296 |
| streptomyces luozhongensis sp. nov., a novel actinomycete with antifungal activity and antibacterial activity. | a novel actinomycete strain, designated trm 49605(t), was isolated from a desert soil sample from lop nur, xinjiang, north-west china, and characterised using a polyphasic taxonomic approach. the strain exhibited antifungal activity against the following strains: saccharomyces cerevisiae, curvularia lunata, aspergillus flavus, aspergillus niger, fusarium oxysporum, penicillium citrinum, candida albicans and candida tropicalis; antibacterial activity against bacillus subtilis, staphylococcus epid ... | 2017 | 27752797 |
| a mini-survey of moulds and mycotoxins in locally grown and imported wheat grains in nigeria. | a preliminary survey involving limited sample size was conducted to determine the spectrum of moulds and mycotoxins in wheat grains from flour mills and local markets in nigeria. fourteen wheat samples were analyzed for moulds using standard mycological methods and for toxic fungal metabolites using a liquid chromatography-tandem mass spectrometric method. fusarium (range of incidence 12.5-61.7%) dominated in the wheat grains though species of aspergillus (range of incidence 2.24-3.86%) were als ... | 2017 | 27905064 |
| synthesis and biological evaluation of newer 1,3,4-oxadiazoles incorporated with benzothiazepine and benzodiazepine moieties. | a series of thiazepines and diazepines having 1,3,4-oxadiazole moiety were synthesized, and they were analyzed for their in vitro antimicrobial activity against several bacteria (staphylococcus aureus, staphylococcus pyogenes, escherichia coli, and pseudomonas aeruginosa) and fungi (candida albicans, aspergillus niger, and aspergillus clavatus) and protozoa (entamoeba histolytica, giardia lamblia, trypanosoma cruzi and leishmania mexicana). few of the selected compounds were tested for their ant ... | 2017 | 28182579 |
| loop 3 of fungal endoglucanases of glycoside hydrolase family 12 modulates catalytic efficiency. | glycoside hydrolase (gh) family 12 comprises enzymes with a wide range of activities critical for the degradation of lignocellulose. however, the important roles of the loop regions of gh12 enzymes in substrate specificity and catalytic efficiency remain poorly understood. this study examined how the loop 3 region affects the enzymatic properties of gh12 glucanases using nfeg12a from neosartorya fischeri p1 and eg (pdb 1ks4) from aspergillus niger acidophilic and thermophilic nfeg12a had the hig ... | 2017 | 28039140 |
| occurrence of fungi and cytotoxicity of the species: aspergillus ochraceus, aspergillus niger and aspergillus flavus isolated from the air of hospital wards. | the basic care requirement for patients with weakened immune systems is to create the environment where the risk of mycosis is reduced to a minimum. | 2017 | 28366953 |
| biocontrol potential of halotolerant bacterial chitinase from high yielding novel bacillus pumilus mcb-7 autochthonous to mangrove ecosystem. | the multifaceted role of chitinase in medicine, agriculture, environmental remediation and various other industries greatly demands the isolation of high yielding chitinase producing microorganisms with improved properties. the current study aimed to investigate the isolation, characterization and biocontrol prospective of chitinase producing bacterial strains autochthonous to the extreme conditions of mangrove ecosystems. among the 51 bacterial isolates screened, bacillus pumilus mcb-7 with hig ... | 2017 | 28364802 |
| stonebrood and chalkbrood in apis mellifera causing fungi: in vitro sensitivity to some essential oils. | aim of the present study was to evaluate the in vitro antimycotic activity of 17 chemically defined essential oils (eos) both alone and as a mixture, against agents responsible for stonebrood caused by aspergillus flavus, and chalkbrood caused by ascosphaera apis in european honeybees. cinnamomum zeylanicum yielded the lowest mic value against a. flavus, but was not effective against a. apis, while litsea cubeba and pelargonium graveolens appeared to be effective against all checked fungi. asper ... | 2017 | 28326846 |
| alkaline technosol contaminated by former mining activity and its culturable autochthonous microbiota. | technosols or technogenic substrates contaminated by potentially toxic elements as a result of iron mining causes not only contamination of the surrounding ecosystem but may also lead to changes of the extent, abundance, structure and activity of soil microbial community. microbial biomass were significantly inhibited mainly by exceeding limits of potentially toxic metals as arsenic (in the range of 343-511 mg/kg), copper (in the range of 7980-9227 mg/kg), manganese (in the range of 2417-2670 mg ... | 2017 | 28006667 |
| psc-afp from psoralea corylifolia l. overexpressed in pichia pastoris increases antimicrobial activity and enhances disease resistance of transgenic tobacco. | psc-afp, isolated from the seeds of psoralea corylifolia l., is an antimicrobial protein with trypsin inhibitor activity. its encoding gene was cloned by 3'- rapid amplification of cdna ends (race) combined with y-shaped adaptor-dependent extension (yade) method. the gene psc-afp encodes a protein of 203 amino acids with a deduced signal peptide of 24 residues. the growth inhibition effect exerted by the heterologously expressed psc-afp in pichia pastoris revealed that the recombinant psc-afp in ... | 2017 | 27587300 |
| evaluation of phenolic compounds and antioxidant and antimicrobial activities of some common herbs. | the study was designed to evaluate the phenolic, flavonoid contents and antioxidant and antimicrobial activities of onion (allium cepa), garlic (allium sativum), mint (mentha spicata), thyme (thymus vulgaris), oak (quercus), aloe vera (aloe barbadensis miller), and ginger (zingiber officinale). all extracts showed a wide range of total phenolic contents, that is, 4.96 to 98.37 mg/100 g gallic acid equivalents, and total flavonoid contents, that is, 0.41 to 17.64 mg/100 g catechin equivalents. an ... | 2017 | 28316626 |
| development of a dot-blot assay for the detection of mould-specific ige in the belgian population. | data on mould sensitization in the general population are scarce and mostly on aspergillus fumigatus, alternaria alternata and cladosporium herbarum. | 2017 | 27878528 |
| synthesis and antimicrobial properties of 4-acylaminobenzenethiosulfoacid s-esters. | a series of esters of 4-acetyl, 4-trifluoroacetyl- and 4-(3-chloropropionyl)aminobenzenethiosulfoacids (twenty-four compounds) were synthesized and characterized by elemental analysis, (1)h nmr and ir spectroscopy. the antibacterial activity of the novel candidates has been screened using the agar diffusion or serial dilution methods against representative gram-positive (staphylococcus aureus, bacillus subtilis, bacillus mesentericus, mycobacterium sp., mycobacterium luteum), gram-negative (aero ... | 2017 | 28344478 |
| isolation and identification of phyllospheric bacteria possessing antimicrobial activity from astragalus obtusifolius, prosopis juliflora, xanthium strumarium and hippocrepis unisiliqousa. | the widespread utilization of antimicrobial compounds has caused emergence of resistant microorganisms in the world. hence, the research to probe the products with antimicrobial features has led to finding natural habitats and discovering new pharmaceutical products. | 2017 | 28090278 |