Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the cell wall subproteome of listeria monocytogenes. | the surface subproteome of listeria monocytogenes that includes many proteins already known to be involved in virulence and interaction with host cells has been characterized. a new method for the isolation of a defined surface proteome of low complexity has been established based on serial extraction of proteins by different salts at high concentration, and in all 55 proteins were identified by n-terminal sequencing and mass spectrometry. about 16% of these proteins are of unknown function and ... | 2004 | 15378750 |
| [rhombencephalitis caused by listeria: clinical-radiological correlation]. | rhombencephalitis due to listeria is a serious and infrequent infection of the brainstem. it principally affects subjects who were previously healthy. it shows itself clinically in two phases: the first with unspecific symptoms, which could last one week, and the second with the appearance of focal neurologic signs at the level of the brainstem. we present the case of a patient with rhombencephalitis due to listeria that began initially with headache, nauseas and fever and after ten days the pat ... | 2004 | 15381957 |
| photochemical treatment of platelet concentrates with amotosalen and long-wavelength ultraviolet light inactivates a broad spectrum of pathogenic bacteria. | bacterial contamination of platelet (plt) concentrates can result in transfusion-transmitted sepsis. a photochemical treatment (pct) process with amotosalen hcl and long-wavelength ultraviolet light (uva), which cross-links nucleic acids, was developed to inactivate bacteria and other pathogens in plt concentrates. | 2004 | 15383024 |
| intestinal epithelial antigen induces mucosal cd8 t cell tolerance, activation, and inflammatory response. | intestinal autoimmune diseases are thought to be associated with a breakdown in tolerance, leading to mucosal lymphocyte activation perhaps as a result of encounter with bacterium-derived ag. to study mucosal cd8(+) t cell activation, tolerance, and polarization of autoimmune reactivity to self-ag, we developed a novel (fabpl(4x at -132)-ova) transgenic mouse model expressing a truncated form of ova in intestinal epithelia of the terminal ileum and colon. we found that ova-specific cd8(+) t cell ... | 2004 | 15383561 |
| growth, virulence, and immunogenicity of listeria monocytogenes aro mutants. | mutants of listeria monocytogenes with deletions in genes of the common branch of the biosynthesis pathway leading to aromatic compounds were constructed as possible virulence-attenuated carrier strains for protein antigens or vaccine dna. aroa, arob, and in particular aroe mutants showed strongly reduced growth rates in epithelial cells and even in rich culture media. the metabolism of the aro mutants under these conditions was predominantly anaerobic. aerobic metabolism and a wild-type growth ... | 2004 | 15385459 |
| effect of listeriolysin o-loaded erythrocytes on mycobacterium avium replication within macrophages. | to evaluate the efficacy of erythrocytes loaded with the haemolytic toxin listeriolysin o against mycobacterium avium replication within human macrophages. | 2004 | 15056645 |
| fulminant listeria monocytogenes meningitis complicated with acute hydrocephalus in healthy children beyond the newborn period. | we describe 3 previously healthy costa rican children who had listeria monocytogenes meningitis, an uncommon cause of bacterial meningitis beyond the newborn period in normal subjects. two of them had initial normal brain computed tomography, but all 3 developed acute hydrocephalus at days 7, 3, and 5, respectively. all required immediate ventriculostomy placement and only 1 of 3 survived. l. monocytogenes should be considered among the etiologies of bacterial meningitis in children who do not r ... | 2004 | 15057178 |
| [investigation of a listeriosis cluster: how complete are laboratory-based reports?]. | in germany, listeriosis surveillance is based on mandatory laboratory notification of listeria monocytogenes. local public health departments (lphds) forward the received reports anonymously to state public health departments (sphds) which send them to the national public health centre. for the 28th reporting week (rw) 2003, lphds reported four cases of listeriosis to the sphd of hesse, cumulating to 19 listeriosis cases in 2003 compared to four cases in rw 1 - 28, 2002. | 2004 | 15609211 |
| [listeria monocytogenes endophthalmitis]. | listeria monocytogenes is a rare cause of endogenous endophthalmitis. controversy exists concerning clinical progress and prognosis in this bacterial infection. however, only a few reports about the clinical features have been previously published in the literature. | 2004 | 15599813 |
| recombinant listeria vaccines containing pest sequences are potent immune adjuvants for the tumor-associated antigen human papillomavirus-16 e7. | previous work in our laboratory has established that the fusion of tumor-associated antigens to a truncated form of the listeria monocytogenes virulence factor listeriolysin o (llo) enhances the immunogenicity and antitumor efficacy of the tumor antigen when delivered by listeria or by vaccinia. llo contains a pest sequence at the nh(2) terminus. these sequences, which are found in eukaryotic proteins with a short cellular half-life, target proteins for degradation in the ubiquitin-proteosome pa ... | 2004 | 15604239 |
| in vitro screening of potential probiotic activities of selected lactobacilli isolated from unpasteurized milk products for incorporation into soft cheese. | the aim was to select potentially probiotic lactobacilli from 88 strains isolated from unpasteurized milk and cheese products, and to incorporate these bacteria in a viable state into a soft cheese, without changing its quality. the survival of these bacteria was assessed in acidic and bile conditions, after freezing at -80 degrees c. four strains from unpasteurized camembert--two lactobacillus plantarum strains and two lb. paracasei/casei strains--were identified and typed by pcr and pfge and w ... | 2004 | 15605712 |
| [initial antibiotic therapy of neonatal sepsis]. | it is certain that in the past the types of bacterial agents responsible for neonatal sepsis and their sensitivity to antibiotics were not the same in all historical periods. however, the reports confirming the conclusion have been published only in the last three years. according to these facts, the bacterial causes of neonatal sepsis were analyzed in patients treated at the university children's hospital in belgrade (s&m) as well as their sensitivity to antibiotics to determine the most effect ... | 2004 | 15615466 |
| influence of environmental conditions on the expression of virulence factors by listeria monocytogenes and their use in species identification. | the hemolytic, lecithinase or phosphatidylinositol-specific phospholipase c activities of listeria monocytogenes can be used to differentiate this pathogenic bacteria from l. innocua, apathogenic, frequently isolated from environmental sources and food. however, the interpretation of these characteristics is problematic because of the variation in the expression of virulence factors by l. monocytogenes, which can be influenced by environmental conditions. we used a cheap, simple plate assay to m ... | 2004 | 15451102 |
| resistance of gram-positive bacteria to nisin is not determined by lipid ii levels. | lipid ii is essential for nisin-mediated pore formation at nano-molar concentrations. we tested whether nisin resistance could result from different lipid ii levels, by comparing the maximal lipid ii pool in micrococcus flavus (sensitive) and listeria monocytogenes (relatively insensitive) and their nisin-resistant variants, with a newly developed method. no correlation was observed between the maximal lipid ii pool and nisin sensitivity, as was further corroborated by using spheroplasts of nisi ... | 2004 | 15451114 |
| inactivation of listeria innocua in nisin-treated salmon (oncorhynchus keta) and sturgeon (acipenser transmontanus) caviar heated by radio frequency. | recent regulatory concerns about the presence of the pathogen listeria monocytogenes in ready-to-eat aquatic foods such as caviar has prompted the development of postpackaging pasteurization processes. however, caviar is heat labile, and conventional pasteurization processes affect the texture, color, and flavor of these foods negatively. in this study, chum salmon (oncorhynchus keta, 2.5% total salt) caviar or ikura and sturgeon (acipenser transmontanus, 3.5% total salt) caviar were inoculated ... | 2004 | 15453574 |
| combining pediocin (alta 2341) with postpackaging thermal pasteurization for control of listeria monocytogenes on frankfurters. | frankfurters packaged in 1-link, 5-link, or 10-link packages were surface-inoculated with a five-strain mixture of listeria monocytogenes (3.40 or 5.20 log cfu/g) after treatments with 3,000 arbitrary units (au) or 6,000 au pediocin (in alta 2341) per link. the frankfurters were vacuum packaged, after which the packages were heated in hot water at 71, 81, or 96 degrees c for 30, 60, or 120 s. l. monocytogenes was enumerated following the treatments. selected treatments were subsequently evaluate ... | 2004 | 15453575 |
| combining pediocin with postpackaging irradiation for control of listeria monocytogenes on frankfurters. | frankfurters, in 1-link, 5-link, or 10-link packages, were surface inoculated with a five-strain mixture of listeria monocytogenes (3.40 or 5.20 log cfu/g) after treatment with 3,000 arbitrary units (au) or 6,000 au of pediocin (in alta 2341) per link. the frankfurters were vacuum packaged, after which the 1-link and 5-link packages were irradiated at 1.2 or 2.3 kgy and the 10-link packages were irradiated at 1.4 or 3.5 kgy. l. monocytogenes was enumerated following the treatments. selected trea ... | 2004 | 15453576 |
| antibacterial effect of water-soluble arrowroot (puerariae radix) tea extracts on foodborne pathogens in ground beef and mushroom soup. | antimicrobial activity of water-soluble arrowroot tea extract was evaluated against escherichia coli o157:h7, salmonella enterica serotype enteritidis, listeria monocytogenes, and staphylococcus aureus in ground beef and mushroom soup. the concentrations of arrowroot tea used were 0, 3, and 6% (wt/wt) for ground beef and 0, 1, 5, and 10% (wt/vol) for mushroom soup. samples without tea extract were considered controls. each sample was stored for 0, 1, 3, 5, and 7 days at 7 degrees c for ground be ... | 2004 | 15453588 |
| a rapid method for determining the antimicrobial activity of novel natural molecules. | preservatives used in the agro-food industries may be of natural origin or obtained chemically. because of the increasing interest of consumers in food products that contain only natural ingredients, studies on preservative molecules of natural origin, such as organic acids or peptides, have been reported in the past several years. such studies, which require numerous assays, may be limited by the large amount of molecules required. microscale assays provide an opportunity for testing natural co ... | 2004 | 15453590 |
| repeated cycles of rapid actin assembly and disassembly on epithelial cell phagosomes. | we have found that early in infection of the intracellular pathogen listeria monocytogenes in madin-darby canine kidney epithelial cells expressing actin conjugated to green fluorescent protein, f-actin rapidly assembles (approximately 25 s) and disassembles (approximately 30 s) around the bacteria, a phenomenon we call flashing. l. monocytogenes strains unable to perform actin-based motility or unable to escape the phagosome were capable of flashing, suggesting that the actin assembly occurs on ... | 2004 | 15456901 |
| immune responses to listeria monocytogenes. | listeria monocytogenes is a gram-positive bacterium that is often used to study the mammalian immune response to infection because it is easy to culture, is relatively safe to work with and causes a highly predictable infection in laboratory mice. the broad application of this mouse model has resulted in a torrent of studies characterizing the contributions of different cytokines, receptors, adaptors and effector molecules to resistance against infection with listeria monocytogenes. these studie ... | 2004 | 15459672 |
| eye infections due to listeria monocytogenes in three cows and one horse. | a retrospective study was conducted to determine case histories, microbiological characteristics, and molecular subtypes associated with listeria monocytogenes infections of the eye in large animals. for selected cases, environmental l. monocytogenes contamination patterns on case farms were also evaluated to probe for potential sources and spread of listerial eye infections. records of 170 l. monocytogenes isolates from animal infections were reviewed to determine the fraction of isolates assoc ... | 2004 | 15460335 |
| foodborne disease outbreaks in australia, 1995 to 2000. | health agencies are increasingly conducting systematic reviews of foodborne disease outbreak investigations to develop strategies to prevent future outbreaks. we surveyed state and territory health departments to summarise the epidemiology of foodborne disease outbreaks in australia from 1995 to 2000. from 1995 through 2000, 293 outbreaks were identified, with 214 being of foodborne origin. one hundred and seventy-four (81%) had a known aetiology, and accounted for 80 per cent (6,472/8,124) of i ... | 2004 | 15460958 |
| flagellin from listeria monocytogenes is glycosylated with beta-o-linked n-acetylglucosamine. | glycan staining of purified flagellin from listeria monocytogenes serotypes 1/2a, 1/2b, 1/2c, and 4b suggested that the flagellin protein from this organism is glycosylated. mass spectrometry analysis demonstrated that the flagellin protein of l. monocytogenes is posttranslationally modified with o-linked n-acetylglucosamine (glcnac) at up to six sites/monomer. the sites of glycosylation are all located in the central, surface-exposed region of the protein monomer. immunoblotting with a monoclon ... | 2004 | 15466023 |
| mechanisms of bactericidal action of cinnamaldehyde against listeria monocytogenes and of eugenol against l. monocytogenes and lactobacillus sakei. | the spice oil components eugenol and cinnamaldehyde possess activity against both gram-positive and gram-negative bacteria, but the mechanisms of action remain obscure. in broth media at 20 degrees c, 5 mm eugenol or 30 mm cinnamaldehyde was bactericidal (>1-log reduction in the number of cfu per milliliter in 1 h) to listeria monocytogenes. at a concentration of 6 mm eugenol was bactericidal to lactobacillus sakei, but treatment with 0.5 m cinnamaldehyde had no significant effect. to investigat ... | 2004 | 15466510 |
| listeria monocytogenes isolates from foods and humans form distinct but overlapping populations. | a total of 502 listeria monocytogenes isolates from food and 492 from humans were subtyped by ecori ribotyping and pcr-restriction fragment length polymorphism analysis of the virulence gene hly. isolates were further classified into genetic lineages based on subtyping results. food isolates were obtained through a survey of selected ready-to-eat food products in maryland and california in 2000 and 2001. human isolates comprised 42 isolates from invasive listeriosis cases reported in maryland an ... | 2004 | 15466521 |
| detection of low levels of listeria monocytogenes cells by using a fiber-optic immunosensor. | biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. an antibody-based fiber-optic biosensor to detect low levels of listeria monocytogenes cells following an enrichment step was developed. the principle of the sensor is a sandwich immunoassay where a rabbit polyclonal antibody was first immobilized on polystyrene fiber waveguides through a biotin-streptavidin reaction to capture listeria cells on the fiber. capture of cel ... | 2004 | 15466560 |
| rapid quantitative detection of listeria monocytogenes in meat products by real-time pcr. | we describe a quick and simple method for the quantitative detection of listeria monocytogenes in meat products. this method is based on filtration, chelex-100-based dna purification, and real-time pcr. it can detect as few as 100 cfu/g and quantify as few as 1,000 cfu/g, with excellent accuracy compared to that of the plate count method. therefore, it is a promising alternative for the detection of l. monocytogenes in meat products. | 2004 | 15466579 |
| listeria monocytogenes--questions and answers. | 2004 | 15468516 | |
| risk assessment strategies for europe: integrated safety strategy or final product control: example of listeria monocytogenes in processed products from pork meat industry. | the european regulation 2160/2003 of november 17th, 2003 clearly shows the european strategy of zoonosis monitoring and control as an integrated approach, including the entire food production chain with a first application to salmonella control in different animal species. this regulation is the consequence of a risk assessment performed with a "farm to fork" philosophy. european strategy is scarcely different from the american strategy, despite the fact that both were achieved by a quantitative ... | 2004 | 15469063 |
| the host resistance locus sst1 controls innate immunity to listeria monocytogenes infection in immunodeficient mice. | epidemiological, clinical, and experimental approaches have convincingly demonstrated that host resistance to infection with intracellular pathogens is significantly influenced by genetic polymorphisms. using a mouse model of infection with virulent mycobacterium tuberculosis (mtb), we have previously identified the sst1 locus as a genetic determinant of host resistance to tuberculosis. in this study we demonstrate that susceptibility to another intracellular pathogen, listeria monocytogenes, is ... | 2004 | 15470055 |
| identification of the antibacterial component of an ethanolic extract of the australian medicinal plant, eremophila duttonii. | activity-guided fractionation was used to determine the antibacterial component of an ethanolic extract of the leaves of an australian native medicinal plant, eremophila duttonii f. muell. (myoporaceae). the extract, previously shown to have activity against gram positive bacteria, was shown to have activity against additional gram positive bacteria, including clostridium perfringens, c. sporogenes and listeria monocytogenes. thin layer chromatography (tlc) was used to separate the extract into ... | 2004 | 15476308 |
| classes and functions of listeria monocytogenes surface proteins. | listeria monocytogenes is an opportunistic pathogen that causes infections collectively termed listeriosis, which are related to the ingestion of food contaminated with these gram-positive rods. the pathogenicity of l. monocytogenes is determined by the following virulence factors: listeriolysin o, protein acta, two phospholipases c, internalins (in1a and in1b), protein cwha and a metalloprotease. the bacterium is a model organism in studies on the pathogenesis of intracellular parasites. it is ... | 2004 | 15478352 |
| antimicrobial and physicochemical properties of chitosan-hpmc-based films. | to prepare composite films from biopolymers with anti-listerial activity and moisture barrier properties, the antimicrobial efficiency of chitosan-hydroxy propyl methyl cellulose (hpmc) films, chitosan-hpmc films associated with lipid, and chitosan-hpmc films chemically modified by cross-linking were evaluated. in addition, the physicochemical properties of composite films were evaluated to determine their potential for food applications. the incorporation of stearic acid into the composite chit ... | 2004 | 15479027 |
| application of antimicrobial ice for reduction of foodborne pathogens (escherichia coli o157:h7, salmonella typhimurium, listeria monocytogenes) on the surface of fish. | the efficacy of antimicrobial ice was evaluated for the reduction of foodborne pathogens on the surface of fish. | 2004 | 15479406 |
| biodiversity of listeria monocytogenes sensitivity to bacteriocin-producing carnobacterium strains and application in sterile cold-smoked salmon. | the aim of this study was to demonstrate the inhibitory capacity of carnobacterium strains against a collection of listeria monocytogenes strains in cold-smoked salmon (css). | 2004 | 15479419 |
| lxr-dependent gene expression is important for macrophage survival and the innate immune response. | the liver x receptors (lxrs) are nuclear receptors with established roles in the regulation of lipid metabolism. we now show that lxr signaling not only regulates macrophage cholesterol metabolism but also impacts antimicrobial responses. mice lacking lxrs are highly susceptible to infection with the intracellular bacteria listeria monocytogenes (lm). bone marrow transplant studies point to altered macrophage function as the major determinant of susceptibility. lxr-null macrophages undergo accel ... | 2004 | 15479645 |
| microbiological quality of pre-cut fruit, sprouted seeds, and unpasteurised fruit and vegetable juices from retail and production premises in the uk, and the application of haacp. | a study of ready-to-eat pre-cut fruit, sprouted seeds, and unpasteurised fruit and vegetable juices from retail and production premises was undertaken in the uk to determine the microbiological quality of these products, and to verify the application of hazard analysis and critical control points (haccp) procedures by food operators. almost all (99%; 2,075/2,096) samples were of satisfactory/acceptable microbiological quality. two (0.1%) samples (melon, beansprouts) were of unacceptable quality ... | 2004 | 15481210 |
| [biocenosis of the natural foci of sapronotic infections (the results of 15-year observations)]. | the summarized results of original studies, carried out in 1988-2003 by scientists of the gamaleya research institute of epidemiology and microbiology, are discussed. the part dealing with the regularity and mechanisms of the circulation of pathogenic bacteria in the biocenosis of soil and water reservoirs includes the following subjects: population and intracellular interactions with protozoa; the evaluation of different representatives of hydrobios as hosts and the transmission along trophic b ... | 2004 | 15481938 |
| five essential oils from aromatic plants of cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of listeria innocua examined by flow cytometry. | to investigate the antibacterial effect of five essential oils (eo) extracted from aromatic plants (cymbopogon citratus, ocimumbasilicum, ocimum gratissimum, thymus vulgaris and zingiber officinale) of cameroon against strains of listeria monocytogenes, l. innocua and staphylococcus aureus. the ability of selected eo to permeabilize the cytoplasmic membrane of l. innocua was also examined. | 2004 | 15482428 |
| antimicrobial activity of ultrasound-assisted solvent-extracted spices. | the objective of this research was to determine the antimicrobial activity of conventional and high-intensity ultrasound-assisted (hi-us) solvent-extracted thai spices, including ginger (zingiber officinale rose), fingerroot (bosenbergia pandurata holtt) and turmeric (curouma longa linn). | 2004 | 15482429 |
| [rapid diagnosis of neonatal sepsis by 16srrna genes pcr amplification and genechip hybridization]. | to explore a method for rapid diagnosis of sepsis in newborn infants. | 2004 | 15482666 |
| a cloned prokaryotic cd2+ p-type atpase increases yeast sensitivity to cd2+. | cada, the p1-type atpase involved in listeria monocytogenes resistance to cd(2+), was expressed in saccharomyces cerevisiae and did just the opposite to what was expected, as it strikingly decreased the cd(2+) tolerance of these cells. yeast cells expressing the non-functional mutant asp(398)ala could grow on selective medium containing up to 100 microm cd(2+), whereas those expressing the functional protein could not grow in the presence of 1 microm cd(2+). the cada-gfp fusion protein was local ... | 2004 | 15485658 |
| molecular determinants of listeria monocytogenes virulence. | listeria monocytogenes is the etiological agent of listeriosis, a severe human foodborne infection characterized by gastroenteritis, meningitis, encephalitis, abortions, and perinatal infections. this gram-positive bacterium is a facultative intracellular pathogen that induces its own uptake into nonphagocytic cells and spreads from cell to cell using an actin-based motility process. this review covers both well-established and recent advances in the characterization of l. monocytogenes virulenc ... | 2004 | 15487949 |
| a totally synthetic vaccine of generic structure that targets toll-like receptor 2 on dendritic cells and promotes antibody or cytotoxic t cell responses. | a simple generic peptide-based vaccine structure that targets toll-like receptor 2-expressing dendritic cells and causes their activation is described. the vaccines are totally synthetic, serve as their own adjuvant, and are composed of (i) a single helper t cell epitope, (ii) a target epitope that is either recognized by cd8+ t cells or b cells, and (iii) a toll-like receptor 2-targeting lipid moiety, s-[2,3-bis(palmitoyloxy)propyl]cysteine, that is situated between the peptide epitopes to form ... | 2004 | 15489266 |
| construction of an enterococcus faecalis tn917-mediated-gene-disruption library offers insight into tn917 insertion patterns. | sequencing the insertion sites of 8,865 tn917 insertions in enterococcus faecalis strain og1rf identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. in e. faecalis, tn917 preferentially inserted at a 29-bp consensus sequence centered on tataa, a 5-bp sequence that is duplicated during insertion. the regional insertion site preference at the chromosome terminus was not observed in another low-g+c gram-positive bacteri ... | 2004 | 15489440 |
| evaluation of vidas listeria monocytogenes ii (lmo2) immunoassay method for the detection of listeria monocytogenes in foods: collaborative study. | a multilaboratory study was conducted to compare the vidas listeria monocytogenes ii (lmo2) immunoassay and the standard cultural methods for the detection of listeria monocytogenes in foods. five food types-vanilla ice cream, brie cheese, cooked roast beef, frozen green beans, and frozen tilapia fish-at 3 levels were analyzed by each method. a total of 26 laboratories representing government and industry participated. in this study, 1404 test portions were analyzed of which 1152 were used in th ... | 2004 | 15493669 |
| lipid rafts clustering and signalling by listeriolysin o. | listeriolysin o, the major virulent determinant of listeria monocytogenes, is known for forming pores on cholesterol-rich membranes. in the present study, we reveal its other facet, rafts clustering. by immunofluorescence microscopy, we show that the glycosylphosphatidylinositol-anchored proteins cd14 and cd24, which normally exhibit uniform distribution on j774 cells, undergo clustering upon treatment with llo. the non-raft marker transferrin receptor is unaffected by such treatment. rafts clus ... | 2004 | 15493995 |
| cross-presentation of listeria-derived cd8 t cell epitopes requires unstable bacterial translation products. | presentation of bacteria-derived cd8 t cell epitopes by dendritic cells (dc) requires either their direct infection or that dc acquire and cross-present ags from other infected cells. we found that cross-presentation of listeria monocytogenes-derived cd8 t cell epitopes was much stronger than direct ag presentation by infected murine dc. cross-presentation of listeria-derived cd8 t cell epitopes showed unique physiological requirements. it was dependent upon the delivery of unstable bacterial tr ... | 2004 | 15494515 |
| reduced apoptosis and ameliorated listeriosis in trail-null mice. | listeriosis is an infectious disease caused by the bacterium listeria monocytogenes. although it is well recognized that apoptosis plays a critical role in the pathogenesis of the disease, the molecular mechanisms of cell death in listeriosis remain to be established. we report in this study that mice deficient in trail were partially resistant to primary listeriosis, and blocking trail with a soluble death receptor 5 markedly ameliorated the disease. the numbers of listeria in the liver and spl ... | 2004 | 15494516 |
| duration of infection and antigen display have minimal influence on the kinetics of the cd4+ t cell response to listeria monocytogenes infection. | the t cell response to infection consists of clonal expansion of effector cells, followed by contraction to memory levels. it was previously thought that the duration of infection determines the magnitude and kinetics of the t cell response. however, recent analysis revealed that transition between the expansion and contraction phases of the ag-specific cd8+ t cell response is not affected by experimental manipulation in the duration of infection or ag display. we studied whether the duration of ... | 2004 | 15494519 |
| listeria monocytogenes-based antibiotic resistance gene-free antigen delivery system applicable to other bacterial vectors and dna vaccines. | plasmids represent a powerful tool to rapidly introduce genes into bacteria and help them reach high expression levels. in vaccine development, with live vaccine vectors, this allows greater flexibility and the ability to induce larger antigen amounts through multiple gene copies. however, plasmid retention often requires antibiotic resistance markers, the presence of which has been discouraged in clinical applications by the food and drug administration. therefore, we developed a listeria monoc ... | 2004 | 15501772 |
| suppression in lung defense responses after bacterial infection in rats pretreated with different welding fumes. | epidemiology suggests that inhalation of welding fumes increases the susceptibility to lung infection. the effects of chemically distinct welding fumes on lung defense responses after bacterial infection were compared. fume was collected during gas metal arc (gma) or flux-covered manual metal arc (mma) welding using two consumable electrodes: stainless steel (ss) or mild steel (ms). the fumes were separated into water-soluble and -insoluble fractions. the gma-ss and gma-ms fumes were found to be ... | 2004 | 15504457 |
| occurrence of listeria monocytogenes in green table olives. | microbiological safety of green table olives from different cultivars, prepared by both the spanish-style and biological methods and fermented with starter cultures of lactic acid bacteria (lactobacillus plantarum, lactobacillus casei, and lactobacillus pentosus), was investigated. the fermentation process was monitored by measuring ph values, titratable acidities, and growth of lactic acid bacteria over time. during fermentation, lactic acid bacteria and yeasts were major microbial populations. ... | 2004 | 15508629 |
| modeling the growth boundary of listeria monocytogenes in ready-to-eat cooked meat products as a function of the product salt, moisture, potassium lactate, and sodium diacetate concentrations. | a central composite response surface design was used to determine the time to growth of listeria monocytogenes as a function of four continuous variables: added sodium chloride (0.8 to 3.6%), sodium diacetate (0 to 0.2%), potassium lactate syrup (60% [wt/wt]; 0.25 to 9.25%), and finished-product moisture (45.5 to 83.5%) in ready-to-eat cured meat products. the design was repeated for ready-to-eat uncured meat products giving a fifth categorical variable for cure status. products were stored at 4 ... | 2004 | 15508630 |
| survival, growth, and thermal resistance of listeria monocytogenes in products containing peanut and chocolate. | outbreaks of listeriosis associated with the consumption of ready-to-eat foods have raised interest in determining growth, survival, and inactivation characteristics of listeria monocytogenes in a wide range of products. a study was undertaken to determine the thermal tolerance of l. monocytogenes in a peanut-based beverage (3.1% fat), whole-fat (3.5%) milk, wholefat (4.0%) and reduced-fat (1.0%) chocolate milk, a chocolate-peanut spread (39% fat), and peanut butter (53% fat). the d60 degrees c ... | 2004 | 15508631 |
| improved quantitative recovery of listeria monocytogenes from stainless steel surfaces using a one-ply composite tissue. | four sampling devices, a sterile environmental sponge (es), a sterile cotton-tipped swab (cs), a sterile calcium alginate fiber-tipped swab (cas), and a one-ply composite tissue (ct), were evaluated for quantitative recovery of listeria monocytogenes from a food-grade stainless steel surface. sterile 304-grade stainless steel plates (6 by 6 cm) were inoculated with approximately 106 cfu/cm2 l. monocytogenes strain scott a and dried for 1 h. the es and ct sampling devices were rehydrated in phosp ... | 2004 | 15508632 |
| efficacy of two cleaning and sanitizing combinations on listeria monocytogenes biofilms formed at low temperature on a variety of materials in the presence of ready-to-eat meat residue. | biofilms in the food-processing industry are a serious concern due to the potential for contamination of food products, which may lead to decreased food quality and safety. the effect of two detergent and sanitizer combinations on the inactivation of listeria monocytogenes biofilms was studied. combination a uses a chlorinated-alkaline, low-phosphate detergent, and dual peracid sanitizer. combination b uses a solvated-alkaline environmental sanitation product and hypochlorite sanitizer. the surv ... | 2004 | 15508633 |
| control of listeria monocytogenes with combined antimicrobials on beef franks stored at 4 degrees c. | contamination of ready-to-eat meat products such as beef franks with listeria monocytogenes has become a major concern for the meat processing industry and an important food safety issue. the objective of this study was to determine the effectiveness of combinations of antimicrobials as aqueous dipping solutions to control l. monocytogenes on vacuum-packaged beef franks stored at 4 degrees c for 3 weeks. commercial beef franks were dipped for 5 min in three antimicrobial solutions: pediocin (6,0 ... | 2004 | 15508647 |
| effects of preparation methods on the microbiological safety of home-dried meat jerky. | historically, drying meats to produce jerky was conisidered to be a safe preservation process and the convenience and flavor of jerky has made it a popular food product for home food preservers. recent outbreaks of foodborne illness related to both home-dried and commercially manufactured jerky have raised concerns about the safety of the product. some traditional home recipes and drying processes were shown to be inadequate to destroy escherichia coli o157, salmonella, staphylococcus aureus, an ... | 2004 | 15508655 |
| outcome of listeria monocytogenes prosthetic valve endocarditis: as bad as it looks? | in 1998 we presented 1 successfully treated case of listeria monocytogenes prosthetic valve endocarditis and made a review of all the cases that had been published to date. we carry out an up-to-date review through pub-med of every case of listeria monocytogenes prosthetic valve endocarditis; mortality rate is calculated and data from several clinical and therapeutical variables are collected; fisher's exact test is used to identify those variables significantly associated with mortality. four o ... | 2004 | 15513394 |
| listeria monocytogenes in horses in iceland. | twenty isolates of listeria monocytogenes associated with five confirmed and four suspected incidents of listeriosis in horses in iceland were characterised by serotyping, pulsed-field gel electrophoresis and ribotyping. semiquantitative estimates of the numbers of l monocytogenes were made on faeces from horses with clinical signs of listeriosis and on grass silage fed to them. large numbers of l monocytogenes were often found in the faeces of horses with severe signs of disease. the 20 isolate ... | 2004 | 15518406 |
| characterization of degu, a response regulator in listeria monocytogenes, involved in regulation of motility and contributes to virulence. | the degu (lmo2515) gene encodes a putative response regulator in the food-borne pathogen listeria monocytogenes. it has 63% amino acid identity to the degu response regulator of bacillus subtilis. we have characterized the degu gene product in l. monocytogenes egd by generation of a deletion mutant. the deltadegu mutant was found to be non-motile in motility plate assay and no flagellin was detected. the mutant was attenuated in challenge of mice. northern blot analysis suggested that the degu g ... | 2004 | 15522505 |
| growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. | twenty-seven honey samples from different floral sources and geographical locations were evaluated for their ability to inhibit the growth of seven food spoilage organisms (alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, geotrichum candidum, lactobacillus acidophilus, penicillium expansum, pseudomonas fluorescens) and five foodborne pathogens (bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, salmonella enterica ser. typhimurium, and staphylococcus aureus) ... | 2004 | 15527912 |
| antimicrobial resistance profile of five major food-borne pathogens isolated from beef, pork and poultry. | this study was performed to evaluate the resistance rate against antimicrobials of food isolates of the five major food-borne pathogens to compare these and to possibly distinguish a pattern. a total of 922 samples of the major meat species (pork, beef and poultry) were analysed for thermophilic campylobacter, salmonella, yersinia enterocolitica, pathogenic escherichia coli and listeria monocytogenes. isolates were subjected to antimicrobial resistance testing by the disc diffusion method. rough ... | 2004 | 15527915 |
| antimicrobial effect of water extract of sumac (rhus coriaria l.) on the growth of some food borne bacteria including pathogens. | the antimicrobial effect of water extracts of sumac (rhus coriaria l.) at concentrations of 0.1%, 0.5%, 1.0%, 2.5% and 5.0% (w/v), non-neutralized and after neutralization to ph 7.2+/-0.1, was studied on the growth of 12 bacterial strains (six gram positive strains and six gram negative strains), mostly food borne including pathogens. it was found to be effective against all the test organisms with gram positive strains being more sensitive than gram negative strains. significant differences (p< ... | 2004 | 15527919 |
| cutting edge: immunity and ifn-gamma production during listeria monocytogenes infection in the absence of t-bet. | the t-box transcription factor t-bet is an important regulator of ifn-gamma production in all cell types and is considered to be essential for the generation of cd4 th1 t cells. ifn-gamma in turn plays a critical role in immunity to many infectious agents. in this study, we demonstrate that t-bet is not required for host resistance to primary listeria monocytogenes (lm) infection. in the innate immune phase, control of lm replication, serum ifn-gamma, and numbers of ifn-gamma-producing nk cells ... | 2004 | 15528324 |
| morphotypic conversion in listeria monocytogenes biofilm formation: biological significance of rough colony isolates. | adherence to a stainless steel surface selected isolates of listeria monocytogenes with enhanced surface colonization abilities and a change in phenotype from the common smooth colony morphology to a succession of rough colony morphotypes. growth in broth culture of the best-adapted, surface-colonizing rough colony morphotype gave a smooth colony revertant. comparative analysis revealed that the smooth and rough variants had similar phenotypic and biochemical characteristics (e.g., identical gro ... | 2004 | 15528535 |
| new vector for efficient allelic replacement in naturally nontransformable, low-gc-content, gram-positive bacteria. | a shuttle vector designated pmad was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. this vector allows, on x-gal (5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. the plasmid was used in staphylococcus aureus, listeria monocytogenes, and bacillus cereus to efficiently c ... | 2004 | 15528558 |
| sigmab-dependent gene induction and expression in listeria monocytogenes during osmotic and acid stress conditions simulating the intestinal environment. | listeria monocytogenes must overcome a variety of stress conditions in the host digestive tract to cause foodborne infections. the alternative sigma factor sigma(b), encoded by sigb, is responsible for regulating transcription of several l. monocytogenes virulence and stress-response genes, including genes that contribute to establishment of gastrointestinal infections. a quantitative rt-pcr assay was used to measure mrna transcript accumulation for the virulence genes inla and bsh, the stress-r ... | 2004 | 15528669 |
| selective discrimination of listeria monocytogenes epidemic strains by a mixed-genome dna microarray compared to discrimination by pulsed-field gel electrophoresis, ribotyping, and multilocus sequence typing. | listeria monocytogenes can cause serious illness in humans, and subsequent epidemiological investigation requires molecular characterization to allow the identification of specific isolates. l. monocytogenes is usually characterized by serotyping and is subtyped by using pulsed-field gel electrophoresis (pfge) or ribotyping. dna microarrays provide an alternative means to resolve genetic differences among isolates, and unlike pfge and ribotyping, microarrays can be used to identify specific gene ... | 2004 | 15528725 |
| bacteria as dna vaccine carriers for genetic immunization. | genetic immunization with plasmid dna vaccines has proven to be a promising tool in conferring protective immunity in various experimental animal models of infectious diseases or tumors. recent research focuses on the use of bacteria, in particular enteroinvasive species, as effective carriers for dna vaccines. attenuated strains of shigella flexneri, salmonella spp., yersinia enterocolitica or listeria monocytogenes have shown to be attractive candidates to target dna vaccines to immunological ... | 2004 | 15532991 |
| bacteriological investigation of infectious keratoconjunctivitis in norwegian sheep. | contagious keratoconjunctivitis is a rather common disease in norwegian sheep. since the knowledge of its aetiology is limited, the present study was performed to determine the microorganisms involved. local veterinarians throughout the country collected conjunctival swabs from both sick (n = 43) and healthy (n = 42) sheep on 15 farms with outbreaks of ovine keratoconjunctivitis, and further from healthy sheep (n = 50) on 17 farms not showing any signs of conjunctival disease. all samples were c ... | 2004 | 15535083 |
| simultaneous and sensitive detection of three foodborne pathogens by multiplex pcr, capillary gel electrophoresis, and laser-induced fluorescence. | the simultaneous detection of staphylococcus aureus, listeria monocytogenes, and salmonella spp. has been approached by a new multiplex pcr-based procedure followed by capillary gel electrophoresis with laser-induced fluorescence detection (multiplex-pcr-cge-lif). as compared to slab gel electrophoresis, the use of cge-lif improved from 10- to 1000-fold the sensitivity of the multiplex pcr analysis, allowing the detection of 2.6 x 10(3) cfu ml(-1) of s. aureus, 570 cfu ml(-1) of l. monocytogenes ... | 2004 | 15537335 |
| interferon-gamma mediates neuronal killing of intracellular bacteria. | neurons can be targets for microbes, which could kill the neurons. just in reverse, we, in this study, report that bacteria can be killed when entering a neuron. primary cultures of foetal mouse hippocampal neurons and a neuronal cell line derived from mouse hypothalamus were infected by listeria monocytogenes. treatment with interferon-gamma (ifn-gamma) did not affect bacterial uptake, but resulted in increased killing of intracellular bacteria, whereas the neuronal cell remained intact. the if ... | 2004 | 15541035 |
| interactions in biofilms between listeria monocytogenes and resident microorganisms from food industry premises. | twenty nine bacterial strains were grown as binary culture biofilms with listeria monocytogenes to assess their influence on the settlement of the latter on stainless steel coupons. most of the strains had been isolated from food processing plants after cleaning and disinfection and were tentatively identified by the apilab plus 3.3.3 database (biomerieux). sixteen of them decreased l. monocytogenes biofilm colony forming units (cfu) counts. three strains, bacillus sp. ccl 9 an unidentified gram ... | 2004 | 15541798 |
| purification, characterization and amino acid sequencing of divergicin m35: a novel class iia bacteriocin produced by carnobacterium divergens m35. | carnobacterium divergens m35, isolated from a commercial sample of frozen smoked mussels, produces a new bacteriocin, divergicin m35, a class iia bacteriocin. divergicin m35 is sensitive to pronase-e, alpha-chymotrypsin and proteinase k, but not to trypsin and withstands thermal treatments up to 121 degrees c for 30 min. divergicin m35 was extracted from the culture supernatant of c. divergens m35 using an sp-sepharose cation-exchange column, desalted and purified on a c18 sep-pack column and fu ... | 2004 | 15541799 |
| factors affecting the antilisterial effects of nisin in milk. | the ability of listeria monocytogenes to proliferate in milk and the antilisterial activities of nisin are well documented. although milk fat was reported to reduce the antimicrobial activities of nisin, there is little information on the influence of milk fat on the antilisterial activities of nisin in refrigerated milk, and whether pasteurization and homogenization influence these activities. fresh, pasteurized, and homogenized milk samples (0.1%, 2.0%, and 3.5% fat) were treated with nisin (0 ... | 2004 | 15541808 |
| direct detection of bacterial pathogens in representative dairy products using a combined bacterial concentration-pcr approach. | to develop a simple, rapid method to concentrate and purify bacteria and their nucleic acids from complex dairy food matrices in preparation for direct pathogen detection using polymerase chain reaction (pcr). | 2004 | 15546401 |
| relationship between inactivation kinetics of a listeria monocytogenes suspension by chlorine and its chlorine demand. | chlorine demand by listeria monocytogenes cells and inactivation of l. monocytogenes by chlorine (0.6-1.0 mg l(-1)) at different temperatures (4, 20 and 30 degrees c) have been investigated in a batch reactor. | 2004 | 15546419 |
| differential inactivation of listeria monocytogenes by d- and l-lactic acid. | to determine inactivation of listeria monocytogenes by the two lactic acid isomers. | 2004 | 15548306 |
| listeria monocytogenes infection-dependent transfer of exogenously added dna to fibroblast cos-1 cells. | the addition of double-stranded circular or linear dna encoding egfp (the enhanced green fluorescent protein) to a listeria -containing infection medium resulted in up to 8.6% cos-1 cells expressing the reporter protein. the transfer of naked dna into host cells upon infection by listeria was found to be dependent on the ability of the bacteria to synthesize internalins and listeriolysin. since no binding of dna to the bacterial cells was detected, dna uptake seems to be the consequence of the s ... | 2004 | 15549391 |
| effects of recovery, plating, and inoculation methods on quantification of escherichia coli o157:h7 and listeria monocytogenes from strawberries. | effects of different recovery and inoculation methods on quantification of escherichia coli o157:h7 and listeria monocytogenes from strawberries were studied. strawberries were spot or dip inoculated with 7 to 8 log cfu per strawberry of each pathogen, air dried for 2 h, and stored for 1, 3, and 7 days at 4 degrees c. the inoculated samples were stomached or washed with phosphate-buffered saline (pbs; ph 7.2) or with modified pbs (ph 8.4). bacterial levels were determined using a direct selectiv ... | 2004 | 15553625 |
| fate of escherichia coli o157:h7 and listeria monocytogenes in strawberry juice and acidified media at different ph values and temperatures. | survival and growth of escherichia coli o157:h7 and listeria monocytogenes in strawberry juice and acidified media at different ph levels (ph 3.4 to 6.8) and temperatures were studied. sterile strawberry juice (ph 3.6) and acidified trypticase soy broth (tsb) media (ph 3.4 to 6.8) were inoculated with approximately 6.7 log cfu/ml e. coli o157:h7 or 7.3 log cfu/ ml l. monocytogenes, incubated for 3 days at 4 and 37 degrees c. bacterial levels were determined after 2 h, 1 day, and 3 days using sur ... | 2004 | 15553626 |
| decontamination of strawberries using batch and continuous chlorine dioxide gas treatments. | efficacy of chlorine dioxide (clo2) gas in reducing escherichia coli o157:h7 and listeria monocytogenes on strawberries was determined using batch and continuous flow clo2 gas treatment systems. effects of continuous clo2 gas treatment on total aerobic plate count, color, and residual clo2 and chlorite on strawberries were also evaluated. strawberries were spot inoculated with 7 to 8 log cfu per strawberry of each pathogen (e. coli o157:h7 and l. monocytogenes), stored for 1 day at 4 degrees c, ... | 2004 | 15553627 |
| control of listeria monocytogenes on frankfurters with antimicrobials in the formulation and by dipping in organic acid solutions. | the antilisterial activity of sodium lactate (sl) and sodium diacetate (sd) was evaluated in a frankfurter formulation and in combination with a dipping treatment into solutions of lactic acid or acetic acid after processing and inoculation. pork frankfurters were formulated with 1.8% sl or 0.25% sd or combinations of 1.8% sl with 0.25 or 0.125% sd. after processing, frankfurters were inoculated (2 to 3 log cfu/cm2) with a 10-strain composite of listeria monocytogenes and left undipped or were d ... | 2004 | 15553628 |
| control of growth and survival of listeria monocytogenes on smoked salmon by combined potassium lactate and sodium diacetate and freezing stress during refrigeration and frozen storage. | in this study, we evaluated the antimicrobial effects of different levels of a potassium lactate (pl) plus sodium diacetate (sda) mixture against the growth and survival of listeria monocytogenes scott a inoculated onto smoked salmon stored at 4, 10, and -20 degrees c. the effect of freezing stress on the growth kinetics of l. monocytogenes scott a on smoked salmon at 4 and 10 degrees c was also investigated. the use of pl+sda at all tested levels (1.5, 3.3, and 5% of a 60% commercial solution o ... | 2004 | 15553629 |
| effect of prepackage and postpackage pasteurization on postprocess elimination of listeria monocytogenes on deli turkey products. | surface pasteurization for inactivation of listeria monocytogenes was evaluated for radiant heat prepackage pasteurization, submersed water postpackage pasteurization, and combinations of the two techniques on various types of ready-to-eat deli turkey products obtained from at least four different manufacturers. products were inoculated either by in-package liquid inoculum or surface sponge-contact with approximately 10(9) cfu of l. monocytogenes. additional testing of radiant heat pasteurizatio ... | 2004 | 15553630 |
| prevalence and typing of listeria monocytogenes in ready-to-eat food products on the belgian market. | listeria monocytogenes is a major concern to producers of ready-to-eat foods because of the high mortality rate associated with listeriosis and the widespread nature of the organism. to investigate the prevalence of this pathogen in different ready-to-eat food products on the belgian market, a variety of 252 ready-to-eat food products, mainly fish and meat products, were analyzed. overall, l. monocytogenes was detected in 23.4% of the samples. the highest prevalence of l. monocytogenes was found ... | 2004 | 15553631 |
| strain-specific differences in the attachment of listeria monocytogenes to alfalfa sprouts. | contamination of fresh produce with listeria monocytogenes has resulted in outbreaks of systemic listeriosis and febrile gastroenteritis. recalls of alfalfa sprouts have occurred due to contamination with l. monocytogenes. alfalfa sprouts were used as a preharvest model to study the interaction with this human pathogen. seventeen strains were assessed for their capacity to colonize alfalfa sprouts, and strain-specific differences (not related to source, serotype, or lineage) were revealed when t ... | 2004 | 15553632 |
| dairy farm reservoir of listeria monocytogenes sporadic and epidemic strains. | identifying the reservoirs of a pathogen is vital for control of sporadic disease and epidemics. listeria monocytogenes is a zoonotic foodborne pathogen that is responsible for 28% of food-related deaths in the united states annually, as well as a major cause of massive product recalls worldwide. to examine the role of the dairy farm as a potential source or reservoir for l. monocytogenes subtypes shown to cause human listeriosis, we compared the pulsed-field gel electrophoresis (pfge) restricti ... | 2004 | 15553633 |
| longitudinal studies on listeria in smoked fish plants: impact of intervention strategies on contamination patterns. | four ready-to-eat smoked fish plants were monitored for 2 years to study listeria contamination patterns and the impact of plant-specific listeria control strategies, including employee training and targeted sanitation procedures, on listeria contamination patterns. samples from the processing plant environment and from raw and finished product were collected monthly and tested for listeria spp. and listeria monocytogenes. before implementation of intervention strategies, 19.2% of raw product sa ... | 2004 | 15553634 |
| efficacy of acidic electrolyzed water ice for pathogen control on lettuce. | acidic electrolyzed water (acew) was used as frozen acew (acew-ice) for inactivation of listeria monocytogenes and escherichia coli o157:h7 on lettuce. acew-ice was prepared from acew with 20, 50, 100, and 200 ppm of available chlorine by freezing at -40 degrees c and generated 30, 70, 150, and 240 ppm of chlorine gas (cl2), respectively. the acew-ice was placed into styrene-foam containers with lettuce samples at 20 degrees c for 24 h. although acew-ice generating 30 ppm cl2 had no effect on l. ... | 2004 | 15553639 |
| antibacterial effect of water-soluble tea extracts on foodborne pathogens in laboratory medium and in a food model. | the microbial inhibition of foodborne pathogens was determined in brain heart infusion broth with 10% (wt/vol) water-soluble extracts of green, jasmine, black, dungglre, and oolong tea against escherichia coli o157:h7, salmonella enterica serovar enteritidis, listeria monocytogenes, and staphylococcus aureus. the mixed culture (approximately 6.0 log cfu/ml), which was composed of the four pathogens, was inoculated into brain heart infusion broth with and without tea extracts. after incubation at ... | 2004 | 15553649 |
| shortening the infectious period does not alter expansion of cd8 t cells but diminishes their capacity to differentiate into memory cells. | following a primary immune response, a portion of effector t cells gives rise to long-lived memory cells. although primary expansion and differentiation of effector cd8 t cells is dictated by a brief exposure to ag, it is unclear whether full memory differentiation is also programmed within the same short window. by carefully modulating the kinetics of listeria monocytogenes infection, we analyzed the requirements for the programming of effector and memory t cell development in vivo. we find tha ... | 2004 | 15557161 |
| immunostimulating properties of intragastrically administered acetobacter-derived soluble branched (1,4)-beta-d-glucans decrease murine susceptibility to listeria monocytogenes. | we previously found that ac-1, an extracellular polysaccharide, produced by acetobacter xylinum and composed of (1,4)-beta-d-glucan with branches of glucosyl residues, showed a strong activity to induce production of interleukin-12 (il-12) p40 and tumor necrosis factor alpha by macrophages in vitro via toll-like receptor 4 (tlr-4) signaling. in the present study, we examined the effect of oral administration of ac-1 on protective immunity against listeria monocytogenes. mice were given ac-1 or p ... | 2004 | 15557623 |
| listeria monocytogenes sigmab contributes to invasion of human intestinal epithelial cells. | the role of sigma(b) in listeria monocytogenes infection of human intestinal epithelial cells was investigated. invasion defects associated with loss of sigma(b) paralleled those of a deltainla strain independently of the sigma(b)-dependent p2(prfa) promoter. concomitantly, amounts of inla transcript and inla protein were significantly decreased in the deltasigb strain. | 2004 | 15557671 |
| effect of environmental factors on the calibration curve (od-cfu) of listeria monocytogenes. | 2004 | 15560205 | |
| in silico reconstitution of listeria propulsion exhibits nano-saltation. | to understand how the actin-polymerization-mediated movements in cells emerge from myriad individual protein-protein interactions, we developed a computational model of listeria monocytogenes propulsion that explicitly simulates a large number of monomer-scale biochemical and mechanical interactions. the literature on actin networks and l. monocytogenes motility provides the foundation for a realistic mathematical/computer simulation, because most of the key rate constants governing actin networ ... | 2004 | 15562315 |
| listeria monocytogenes in products of animal origin in turkey. | a study was carried out on 430 samples of different foodstuffs (soft cheese, raw chicken, minced beef, sausage, fish) and 400 carcase samples (sheep, young and adult cattle) for screening of listeria monocytogenes. it was found that only one of the samples contained l. monocytogenes at > 10(3) cfu/ml in the initial examination, but another 42 samples contained l. monocytogenes following an enrichment process. l. monocytogenes was isolated most frequently from raw chicken samples (18%), but was n ... | 2004 | 15563103 |