Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| homologous recombination in agrobacterium: potential implications for the genomic species concept in bacteria. | according to current taxonomical rules, a bona fide bacterial species is a genomic species characterized by the genomic similarity of its members. it has been proposed that the genomic cohesion of such clusters may be related to sexual isolation, which limits gene flow between too divergent bacteria. homologous recombination is one of the most studied mechanisms responsible for this genetic isolation. previous studies on several bacterial models showed that recombination frequencies decreased ex ... | 2009 | 18936442 |
| allene oxide cyclase from camptotheca acuminata improves tolerance against low temperature and salt stress in tobacco and bacteria. | allene oxide cyclase (aoc, e 5.3.99.6) is an essential enzyme in jasmonate (ja) biosynthetic pathway. an aoc gene (defined as caaoc, database accession no. ay863428) had been isolated from camptotheca acuminata in previous work. real-time quantitative pcr analysis indicated that mrna expression of caaoc was induced by salt stress (120 mm nacl) and low temperature (4 degrees c). in order to further investigate the role of aoc gene in the processes, caaoc was introduced into tobacco via agrobacter ... | 2009 | 18850307 |
| atrazine removal in agricultural infiltrate by bioaugmented polyvinyl alcohol immobilized and free agrobacterium radiobacter j14a: a sand column study. | bench-scale sand column breakthrough experiments were conducted to examine atrazine removal in agricultural infiltrate by agrobacterium radiobacter j14a (j14a) immobilized in phosphorylated-polyvinyl alcohol compared to free j14a cells. the effects of cell loading and infiltration rate on atrazine degradation and the loss of j14a were investigated. four sets of experiments, (i) tracers, (ii) immobilized dead cells, (iii) immobilized cells, and (iv) free cells, were performed. the atrazine biodeg ... | 2009 | 18848714 |
| characterization of ornithine and glutamine lipids extracted from cell membranes of rhodobacter sphaeroides. | the identification and structural characterization of a series of ornithine lipids extracted from the cell membranes of wild-type rhodobacter sphaeroides, as well as from a glycerophosphocholine-deficient strain, have been achieved by multistage tandem mass spectrometry of their protonated and deprotonated precursor ions in a linear quadrupole ion trap. systematic examination of the multistage gas-phase fragmentation reactions of these ions, combined with the use of hydrogen/deuterium exchange, ... | 2009 | 18835523 |
| characterization of ornithine and glutamine lipids extracted from cell membranes of rhodobacter sphaeroides. | the identification and structural characterization of a series of ornithine lipids extracted from the cell membranes of wild-type rhodobacter sphaeroides, as well as from a glycerophosphocholine-deficient strain, have been achieved by multistage tandem mass spectrometry of their protonated and deprotonated precursor ions in a linear quadrupole ion trap. systematic examination of the multistage gas-phase fragmentation reactions of these ions, combined with the use of hydrogen/deuterium exchange, ... | 2009 | 18835523 |
| genetic transformation and regeneration of sesbania drummondii using cotyledonary nodes. | sesbania drummondii (rydb.) cory is a source for phytopharmaceuticals. it also hyperaccumulates several toxic heavy metals. development of an efficient gene transfer method is an absolute requirement for the genetic improvement of this plant with more desirable traits due to limitations in conventional breeding methods. a simple protocol was developed for agrobacterium-mediated stable genetic transformation of sesbania. agrobacterium tumefaciens strain eha 101 containing the vector pcambia 1305. ... | 2009 | 18825383 |
| the promoter of zmmrp-1, a maize transfer cell-specific transcriptional activator, is induced at solute exchange surfaces and responds to transport demands. | transfer cells have specializations that facilitate the transport of solutes across plant exchange surfaces. zmmrp-1 is a maize (zea mays) endosperm transfer cell-specific transcriptional activator that plays a central role in the regulatory pathways controlling transfer cell differentiation and function. the present work investigates the signals controlling the expression of zmmrp-1 through the production of transgenic lines of maize, arabidopsis, tobacco and barley containing zmmrp-1promoter:g ... | 2009 | 18830706 |
| the promoter of zmmrp-1, a maize transfer cell-specific transcriptional activator, is induced at solute exchange surfaces and responds to transport demands. | transfer cells have specializations that facilitate the transport of solutes across plant exchange surfaces. zmmrp-1 is a maize (zea mays) endosperm transfer cell-specific transcriptional activator that plays a central role in the regulatory pathways controlling transfer cell differentiation and function. the present work investigates the signals controlling the expression of zmmrp-1 through the production of transgenic lines of maize, arabidopsis, tobacco and barley containing zmmrp-1promoter:g ... | 2009 | 18830706 |
| the expression of analgesic-antitumor peptide (agap) from chinese buthus martensii karsch in transgenic tobacco and tomato. | the present study aimed to obtain analgesic-antitumor peptide (agap) gene expression in plants. the analgesic-antitumor peptide (agap) gene was from the venom of buthus martensii karsch. previous studies showed that agap has both analgesic and antitumor activities, suggesting that agap would be useful in clinical situations as an antitumor drug. given that using a plant as an expression vector has more advantages than prokaryotic expression, we tried to obtain transgenic plants containing agap. ... | 2009 | 18546060 |
| variation of 16s-23s internally transcribed spacer sequence and intervening sequence in rdna among the three major agrobacterium species. | we analyzed 16s-23s internally transcribed spacer (its) and neighboring sequences among 37 strains belonging to the three major pathogenic agrobacterium species, in order to know variation in each species and to develop a simple discrimination method. number of its size variation was 9, 4, and 7 in agrobacterium tumefaciens, agrobacterium vitis, and agrobacterium rhizogenes, respectively. the its sequence of most strains in each species was distinguishable from that of the other two species. the ... | 2009 | 17997085 |
| the vibrio cholerae mrp system: cation/proton antiport properties and enhancement of bile salt resistance in a heterologous host. | the mrp operon from vibrio cholerae encoding a putative multisubunit na(+)/h(+) antiporter was cloned and functionally expressed in the antiporter-deficient strain of escherichia coli ep432. cells of ep432 expressing vc-mrp exhibited resistance to na(+) and li(+) as well as to natural bile salts such as sodium cholate and taurocholate. when assayed in everted membrane vesicles of the e. coli ep432 host, vc-mrp had sufficiently high antiport activity to facilitate the first extensive analysis of ... | 2009 | 18311075 |
| the vibrio cholerae mrp system: cation/proton antiport properties and enhancement of bile salt resistance in a heterologous host. | the mrp operon from vibrio cholerae encoding a putative multisubunit na(+)/h(+) antiporter was cloned and functionally expressed in the antiporter-deficient strain of escherichia coli ep432. cells of ep432 expressing vc-mrp exhibited resistance to na(+) and li(+) as well as to natural bile salts such as sodium cholate and taurocholate. when assayed in everted membrane vesicles of the e. coli ep432 host, vc-mrp had sufficiently high antiport activity to facilitate the first extensive analysis of ... | 2009 | 18311075 |
| production of the p24 capsid protein from hiv-1 subtype c in arabidopsis thaliana and daucus carota using an endoplasmic reticulum-directing sekdel sequence in protein expression constructs. | an optimized gene expression construct was designed in order to increase the accumulation of the hiv-1 subtype c p24 protein in arabidopsis thaliana and carrot (daucus carota) plants. an er retention signal was introduced into the genetic construct generating a p24 protein containing a sekdel amino acid sequence at its c-terminus. mature a. thaliana plants and carrot cells were transformed using agrobacterium tumefaciens carrying the improved pgreen0229/p24_sekdel vector. several transgenic plan ... | 2009 | 19167502 |
| roles of agrobacterium tumefaciens rira in iron regulation, oxidative stress response, and virulence. | the analysis of genetics and physiological functions of agrobacterium tumefaciens rira (rhizobial iron regulator) has shown that it is a transcription regulator and a repressor of iron uptake systems. the rira mutant strain (ntlrira) overproduced siderophores and exhibited a highly constitutive expression of genes involved in iron uptake (fhua, irp6a, and fbpa) compared to that of the wild-type strain (ntl4). the deregulation in the iron control of iron uptake in ntlrira led to iron overload in ... | 2009 | 19168612 |
| the signal peptide peptidase is required for pollen function in arabidopsis. | the signal peptide peptidases (spp) are members of the intramembrane cleaving proteases, which are involved in an array of protein-processing and intracellular signaling events in animals. arabidopsis (arabidopsis thaliana) has six genes encoding spp-like proteins, the physiological functions of which are unknown. as a first step in defining the roles of the spps in plants, we examined the distribution and activities of arabidopsis spp (atspp; accession no. at2g03120), the spp-like gene with the ... | 2009 | 19168645 |
| reconfiguring the quorum-sensing regulator sdia of escherichia coli to control biofilm formation via indole and n-acylhomoserine lactones. | sdia is a homolog of quorum-sensing regulators that detects n-acylhomoserine lactone (ahl) signals from other bacteria. escherichia coli uses sdia to reduce its biofilm formation in the presence of both ahls and its own signal indole. here we reconfigured sdia (240 amino acids) to control biofilm formation using protein engineering. four sdia variants were obtained with altered biofilm formation, including truncation variants sdia1e11 (f7l, f59l, y70c, m94k, and k153x) and sdia14c3 (w9r, p49t, n ... | 2009 | 19168658 |
| agrobacterium-mediated transformation of embryogenic tissues of hybrid firs (abies spp.) and regeneration of transgenic emblings. | a genetic transformation system has been developed for selected embryogenic cell lines of hybrids abies alba x a. cephalonica (cell lines ac2, ac78) and abies alba x a. numidica (cell line an72) using agrobacterium tumefaciens. the cell lines were derived from immature or mature zygotic embryos on dcr medium containing ba (1 mg l(-1)). the t-dna of plant transformation vector contained the beta-glucuronidase reporter gene under the control of double dcamv 35s promoter and the neomycin phosphotra ... | 2009 | 19169892 |
| real-time pcr quantification and live-cell imaging of endophytic colonization of barley (hordeum vulgare) roots by fusarium equiseti and pochonia chlamydosporia. | *new tools were developed for the study of the endophytic development of the fungal species fusarium equiseti and pochonia chlamydosporia in barley (hordeum vulgare) roots. these were applied to monitor the host colonization patterns of these potential candidates for biocontrol of root pathogens. * molecular beacons specific for either f. equiseti or p. chlamydosporia were designed and used in real-time polymerase chain reaction (pcr) quantification of fungal populations in roots. genetic transf ... | 2009 | 19170898 |
| use of folding modulators to improve heterologous protein production in escherichia coli. | abstract: despite the fundamental importance of e. coli in the manufacture of a wide range of biotechnological and biomedical products, extensive process and/or target optimisation is routinely required in order to achieve functional yields in excess of low mg/l levels. molecular chaperones and folding catalysts appear to present a panacea for problems of heterologous protein folding in the organism, due largely to their broad substrate range compared with, e.g., protein-specific mutagenesis app ... | 2009 | 19173718 |
| enzyme activities and subcellular localization of members of the arabidopsis glutathione transferase superfamily. | enzyme screens with strep-tagged recombinant proteins and expression studies with the respective green fluorescent protein (gfp) fusions have been employed to examine the functional activities and subcellular localization of members of the arabidopsis glutathione transferase (gst) superfamily. fifty-one of 54 gst family members were transcribed and 41 found to express as functional glutathione-dependent enzymes in escherichia coli. functional redundancy was observed and in particular three theta ... | 2009 | 19174456 |
| roles of dna adenine methylation in host-pathogen interactions: mismatch repair, transcriptional regulation, and more. | the dna adenine methyltransferase (dam methylase) of gammaproteobacteria and the cell cycle-regulated methyltransferase (ccrm) methylase of alphaproteobacteria catalyze an identical reaction (methylation of adenosine moieties using s-adenosyl-methionine as a methyl donor) at similar dna targets (gatc and gantc, respectively). dam and ccrm are of independent evolutionary origin. each may have evolved from an ancestral restriction-modification system that lost its restriction component, leaving an ... | 2009 | 19175412 |
| eukaryotic protein production in designed storage organelles. | protein bodies (pbs) are natural endoplasmic reticulum (er) or vacuole plant-derived organelles that stably accumulate large amounts of storage proteins in seeds. the proline-rich n-terminal domain derived from the maize storage protein gamma zein (zera) is sufficient to induce pbs in non-seed tissues of arabidopsis and tobacco. this zera property opens up new routes for high-level accumulation of recombinant proteins by fusion of zera with proteins of interest. in this work we extend the advant ... | 2009 | 19175916 |
| polar localization of virulence-related esx-1 secretion in mycobacteria. | the esx-1 (type vii) secretion system is critical for virulence of both mycobacterium tuberculosis and mycobacterium marinum, and is highly conserved between the two species. despite its importance, there has been no direct visualization of esx-1 secretion until now. in m. marinum, we show that secretion of mh3864, a novel esx-1 substrate that remains partially cell wall-associated after translocation, occurred in polar regions, indicating that esx-1 secretion takes place in these regions. analy ... | 2009 | 19180234 |
| pathway of gamma-aminobutyrate metabolism in rhizobium leguminosarum 3841 and its role in symbiosis. | pea plants incubated in 15n2 rapidly accumulated labeled gamma-aminobutyrate (gaba) in the plant cytosol and in bacteroids of rhizobium leguminosarum bv. viciae 3841. two pathways of gaba metabolism were identified in r. leguminosarum 3841. in the first, glutamate is formed by gaba aminotransferase (gabt), transferring the amino group from gaba to 2-oxoglutarate. in the second, alanine is formed by two omega-aminotransferases (opaa and opab), transferring the amino group from gaba to pyruvate. w ... | 2009 | 19181799 |
| different biochemical mechanisms ensure network-wide balancing of reducing equivalents in microbial metabolism. | to sustain growth, the catabolic formation of the redox equivalent nadph must be balanced with the anabolic demand. the mechanisms that ensure such network-wide balancing, however, are presently not understood. based on 13c-detected intracellular fluxes, metabolite concentrations, and cofactor specificities for all relevant central metabolic enzymes, we have quantified catabolic nadph production in agrobacterium tumefaciens, bacillus subtilis, escherichia coli, paracoccus versutus, pseudomonas f ... | 2009 | 19181802 |
| in vitro characterization of the enzyme properties of the phospholipid n-methyltransferase pmta from agrobacterium tumefaciens. | agrobacterium tumefaciens requires phosphatidylcholine (pc) in its membranes for plant infection. the phospholipid n-methyltransferase pmta catalyzes all three transmethylation reactions of phosphatidylethanolamine (pe) to pc via the intermediates monomethylphosphatidylethanolamine (mmpe) and dimethylphosphatidylethanolamine (dmpe). the enzyme uses s-adenosylmethionine (sam) as the methyl donor, converting it to s-adenosylhomocysteine (sah). little is known about the activity of bacterial pmt en ... | 2009 | 19181804 |
| construction of disarmed ti plasmids transferable between escherichia coli and agrobacterium species. | agrobacterium-mediated plant transformation has been used widely, but there are plants that are recalcitrant to this type of transformation. this transformation method uses bacterial strains harboring a modified tumor-inducing (ti) plasmid that lacks the transfer dna (t-dna) region (disarmed ti plasmid). it is desirable to develop strains that can broaden the host range. a large number of agrobacterium strains have not been tested yet to determine whether they can be used in transformation. in o ... | 2009 | 19181833 |
| heterologous expression analyses of rice oscas in arabidopsis and in yeast provide evidence for its roles in cyanide detoxification rather than in cysteine synthesis in vivo. | while most dicot plants produce little ethylene in their vegetative stage, many monocots such as rice liberate a relatively large amount of ethylene with cyanide as a co-product in their seedling stage when etiolated. one of the known functions of beta-cyanoalanine synthase (cas) is to detoxify the co-product cyanide during ethylene biosynthesis in higher plants. based on a tryptic peptide sequence obtained from a partially purified cas activity protein preparation in etiolated rice seedlings, t ... | 2009 | 19181864 |
| haca-dependent transcriptional switch releases haca mrna from a translational block upon endoplasmic reticulum stress. | activation of the unfolded protein response (upr) in eukaryotes involves the splicing of an unconventional intron from the mrna encoding the transcriptional activator of the pathway. in saccharomyces cerevisiae a 252-nucleotide (nt) unconventional intron is spliced out of the transcript of hac1, changing the 3' end of the hac1 open reading frame and relieving the transcript from a translational block in a single step. the translational block is caused by the base pairing of part of the unconvent ... | 2009 | 19181870 |
| sequence mining and transcript profiling to explore cyst nematode parasitism. | cyst nematodes are devastating plant parasites that become sedentary within plant roots and induce the transformation of normal plant cells into elaborate feeding cells with the help of secreted effectors, the parasitism proteins. these proteins are the translation products of parasitism genes and are secreted molecular tools that allow cyst nematodes to infect plants. | 2009 | 19183474 |
| rapid system for evaluating bioproduction capacity of complex pharmaceutical proteins in plants. | transgene product yield remains a key limitation in commercializing plant-derived pharmaceutical proteins. although significant progress has been made in understanding the roles of promoters, enhancers, integration sites, codon usage, cryptic rna sites, silencing, and product compartmentalization on product yield and quality, researchers still cannot reliably predict which proteins will be produced at high levels or what manipulations will guarantee enhanced productivity. we have optimized a sim ... | 2009 | 19183893 |
| genome sequence of desulfobacterium autotrophicum hrm2, a marine sulfate reducer oxidizing organic carbon completely to carbon dioxide. | sulfate-reducing bacteria (srb) belonging to the metabolically versatile desulfobacteriaceae are abundant in marine sediments and contribute to the global carbon cycle by complete oxidation of organic compounds. desulfobacterium autotrophicum hrm2 is the first member of this ecophysiologically important group with a now available genome sequence. with 5.6 megabasepairs (mbp) the genome of db. autotrophicum hrm2 is about 2 mbp larger than the sequenced genomes of other sulfate reducers (srb). a h ... | 2009 | 19187283 |
| atrazine degradation by stable mixed cultures enriched from agricultural soil and their characterization. | the aim of this work was to enrich stable mixed cultures from atrazine-contaminated soil. the cultures were examined for their atrazine biodegradation efficiencies in comparison with j14a, a known atrazine-degrading strain of agrobacterium radiobacter. the cultures were also characterized to identify community structure and bacterial species present. | 2009 | 19191954 |
| a cis element within flowering locus t mrna determines its mobility and facilitates trafficking of heterologous viral rna. | the arabidopsis flowering locus t (ft) gene encodes the mobile florigen essential for floral induction. while movement of the ft protein has been shown to occur within plants, systemic spread of ft mrna remains to be unequivocally demonstrated. utilizing novel rna mobility assay vectors based on two distinct movement-defective viruses, potato virus x and turnip crinkle virus, and an agroinfiltration assay, we demonstrate that nontranslatable ft mrna, independent of the ft protein, moves througho ... | 2009 | 19193810 |
| antisense phenotypes reveal a functional expression of osarf1, an auxin response factor, in transgenic rice. | osarf1 is the first full-length member of auxin response factor (arf) gene family to be cloned from monocot plant. using quantitative rt-pcr this study found that, the transcript abundance of osarf1 was significantly higher in embryonic tissues than in vegetative tissues. to investigate the effect of osarf1 on the phenotype of rice, a cdna fragment of osarf1 was inserted in inverse orientation to the 35s promoter in vector pbin438 to produce an antisense (as) construction. the as-osarf1 construc ... | 2009 | 19193962 |
| overexpressed glutamine synthetase gene modifies nitrogen metabolism and abiotic stress responses in rice. | glutamine synthetase (gs; ec 6.3.1.2) is a key enzyme in nitrogen metabolism; it catalyzes the critical incorporation of inorganic ammonium into glutamine. two full-length cdnas that encode the rice (oryza sativa) cytosolic glutamine synthetase1 genes (osgs1;1 and osgs1;2) were isolated from a minghui 63 normalized cdna library, and glna encoding gs in escherichia coli was isolated by pcr amplification. transformants for gs gene (gs1;1, gs1;2, and glna) in rice were produced by an agrobacterium ... | 2009 | 19123004 |
| involvement of shewanella oneidensis mr-1 luxs in biofilm development and sulfur metabolism. | the role of luxs in shewanella oneidensis mr-1 has been examined by transcriptomic profiling, biochemical, and physiological experiments. the results indicate that a mutation in luxs alters biofilm development, not by altering quorum-sensing abilities but by disrupting the activated methyl cycle (amc). the s. oneidensis wild type can produce a luminescence response in the ai-2 reporter strain vibrio harveyi mm32. this luminescence response is abolished upon the deletion of luxs. the deletion of ... | 2009 | 19124589 |
| an hpv 16 l1-based chimeric human papilloma virus-like particles containing a string of epitopes produced in plants is able to elicit humoral and cytotoxic t-cell activity in mice. | even though two prophylactic vaccines against hpv are currently licensed, infections by the virus continue to be a major health problem mainly in developing countries. the cost of the vaccines limits wide-scale application in poor countries. a promising strategy for producing affordable and efficient vaccines involves the expression of recombinant immunogens in plants. several hpv genes have been expressed in plants, including l1, which can self-assemble into virus-like particles. a plant-based, ... | 2009 | 19126233 |
| dihydroorotase from the hyperthermophile aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis. | in prokaryotes, the first three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (cps), aspartate transcarbamoylase (atc), and dihydroorotase (dho), are commonly expressed separately and either function independently (escherichia coli) or associate into multifunctional complexes (aquifex aeolicus). in mammals the enzymes are expressed as a single polypeptide chain (cad) in the order cps-dho-atc and associate into a hexamer. this study presents the three-dimensional structure of ... | 2009 | 19128030 |
| dynamic intracomplex heterogeneity of phytochrome. | low temperature single-molecule fluorescence emission spectroscopy on individual phytochromes from agrobacterium tumefaciens corroborates findings from ensemble spectroscopy concerning intercomplex heterogeneity. furthermore, time-dependent intracomplex heterogeneity has been observed. | 2009 | 19128172 |
| forca, a promoter element that responds to crosstalk between defense and light signaling. | recognition of pathogenic microorganisms triggers in plants comprehensive transcriptional reprogramming. in order to identify transcriptome-level control elements required for plant immune responses we are examining several sets of genes found by microarray experiments to be co-activated in arabidopsis thaliana (arabidopsis) seedlings infected with the oomycete hyaloperonospora parasitica. promoter motifs conserved in clusters of co-expressed genes may be involved in mediating coordinated gene a ... | 2009 | 19128484 |
| genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils. | arsenic is known as a toxic metalloid, which primarily exists in inorganic form [as(iii) and as(v)] and can be transformed by microbial redox processes in the natural environment. as(iii) is much more toxic and mobile than as(v), hence microbial arsenic redox transformation has a major impact on arsenic toxicity and mobility which can greatly influence the human health. our main purpose was to investigate the distribution and diversity of microbial arsenite-resistant species in three different a ... | 2009 | 19128515 |
| tobacco arp3 is localized to actin-nucleating sites in vivo. | the polarity of actin is a central determinant of intracellular transport in plant cells. to visualize actin polarity in living plant cells, the tobacco homologue of the actin-related protein 3 (arp3) was cloned and a fusion with the red fluorescent protein (rfp) was generated. upon transient expression of these fusions in the tobacco cell line by-2 (nicotiana tabacum l. cv. bright yellow 2), punctate structures were observed near the nuclear envelope and in the cortical plasma. these dots could ... | 2009 | 19129161 |
| cell culture-induced gradual and frequent epigenetic reprogramming of invertedly repeated tobacco transgene epialleles. | using a two-component transgene system involving two epiallelic variants of the invertedly repeated transgenes in locus 1 (lo1) and a homologous single-copy transgene locus 2 (lo2), we have studied the stability of the methylation patterns and trans-silencing interactions in cell culture and regenerated tobacco (nicotiana tabacum) plants. the posttranscriptionally silenced (ptgs) epiallele of the lo1 trans-silences and trans-methylates the target lo2 in a hybrid (lo1/lo2 line), while its transcr ... | 2009 | 19129419 |
| identification of rhodococcus fascians cytokinins and their modus operandi to reshape the plant. | decades ago, the importance of cytokinins (cks) during rhodococcus fascians pathology had been acknowledged, and an isopentenyltransferase gene had been characterized in the fas operon of the linear virulence plasmid, but hitherto, no specific ck(s) could be associated with virulence. we show that the ck receptors ahk3 and ahk4 of arabidopsis thaliana are essential for symptom development, and that the ck perception machinery is induced upon infection, underlining its central role in the symptom ... | 2009 | 19129491 |
| the arabidopsis g-protein beta-subunit is required for defense response against agrobacterium tumefaciens. | typical early pathogen-associated molecular pattern (pamp) responses include the generation of reactive oxygen species (ros) and map kinase (mapk) activation, but little is known about the molecular mechanisms that link receptor activation to intracellular signal transduction. in this study, we found that in agb1-2 (agb1 null mutation) mutants, ros production triggered by flg22 or elf18 was significantly reduced and that elf18-stimulated pamp-triggered immunity (pti) against agrobacterium tumefa ... | 2009 | 19129659 |
| remodelling of vipa/vipb tubules by clpv-mediated threading is crucial for type vi protein secretion. | the recently identified type vi secretion systems (t6ss) have a crucial function in the virulence of various proteobacteria, including the human pathogen vibrio cholerae. t6ss are encoded by a conserved gene cluster comprising approximately 15 open reading frames, mediating the appearance of hcp and vgrg proteins in cell culture supernatants. here, we analysed the function of the v. cholerae t6ss member clpv, a specialized aaa+ protein. clpv is crucial for a functional t6ss and interacts through ... | 2009 | 19131969 |
| discovery of pathogenicity genes in the crucifer anthracnose fungus colletotrichum higginsianum, using random insertional mutagenesis. | agrobacterium tumefaciens-mediated transformation (atmt) was used for random insertional mutagenesis to identify pathogenicity genes in the hemibiotrophic fungus colletotrichum higginsianum. a high-throughput primary infection assay on arabidopsis thaliana seedlings allowed the rapid screening of 8,850 transformants. forty mutants showing reproducible pathogenicity defects on arabidopsis and brassica plants were obtained, and their infection phenotypes were characterized microscopically. six mut ... | 2009 | 19132867 |
| characterization of a coxiella burnetii ftsz mutant generated by himar1 transposon mutagenesis. | coxiella burnetii is a gram-negative obligate intracellular bacterium and the causative agent of human q fever. the lack of methods to genetically manipulate c. burnetii significantly impedes the study of this organism. we describe here the cloning and characterization of a c. burnetii ftsz mutant generated by mariner-based himar1 transposon (tn) mutagenesis. c. burnetii was coelectroporated with a plasmid encoding the himar1 c9 transposase variant and a plasmid containing a himar1 transposon en ... | 2009 | 19114492 |
| characterization of a coxiella burnetii ftsz mutant generated by himar1 transposon mutagenesis. | coxiella burnetii is a gram-negative obligate intracellular bacterium and the causative agent of human q fever. the lack of methods to genetically manipulate c. burnetii significantly impedes the study of this organism. we describe here the cloning and characterization of a c. burnetii ftsz mutant generated by mariner-based himar1 transposon (tn) mutagenesis. c. burnetii was coelectroporated with a plasmid encoding the himar1 c9 transposase variant and a plasmid containing a himar1 transposon en ... | 2009 | 19114492 |
| acid and base stress and transcriptomic responses in bacillus subtilis. | acid and base environmental stress responses were investigated in bacillus subtilis. b. subtilis ag174 cultures in buffered potassium-modified luria broth were switched from ph 8.5 to ph 6.0 and recovered growth rapidly, whereas cultures switched from ph 6.0 to ph 8.5 showed a long lag time. log-phase cultures at ph 6.0 survived 60 to 100% at ph 4.5, whereas cells grown at ph 7.0 survived <15%. cells grown at ph 9.0 survived 40 to 100% at ph 10, whereas cells grown at ph 7.0 survived <5%. thus, ... | 2009 | 19114526 |
| acid and base stress and transcriptomic responses in bacillus subtilis. | acid and base environmental stress responses were investigated in bacillus subtilis. b. subtilis ag174 cultures in buffered potassium-modified luria broth were switched from ph 8.5 to ph 6.0 and recovered growth rapidly, whereas cultures switched from ph 6.0 to ph 8.5 showed a long lag time. log-phase cultures at ph 6.0 survived 60 to 100% at ph 4.5, whereas cells grown at ph 7.0 survived <15%. cells grown at ph 9.0 survived 40 to 100% at ph 10, whereas cells grown at ph 7.0 survived <5%. thus, ... | 2009 | 19114526 |
| functional characterization of a stilbene synthase gene using a transient expression system in planta. | the expression and functionality of a resveratrol synthase (rs) gene from peanut (arachis hypogaea) was studied using an agrobacterium tumefaciens-mediated transient expression system in nicotiana benthamiana leaves. functional analysis of rs was demonstrated by tracking its expression during 96 h. to measure the transcripts levels of rs transgene, real-time qrt-pcr was used and revealed that the highest level of transcripts was at 48 h post-transfection. western blot analyses showed that rs pro ... | 2009 | 19116720 |
| an integrated genomics approach to define niche establishment by rhodococcus fascians. | rhodococcus fascians is a gram-positive phytopathogen that induces shooty hyperplasia on its hosts through the secretion of cytokinins. global transcriptomics using microarrays combined with profiling of primary metabolites on infected arabidopsis (arabidopsis thaliana) plants revealed that this actinomycete modulated pathways to convert its host into a niche. the transcript data demonstrated that r. fascians leaves a very characteristic mark on arabidopsis with a pronounced cytokinin response i ... | 2009 | 19118125 |
| rhizobia with different symbiotic efficiencies nodulate acaciella angustissima in mexico, including sinorhizobium chiapanecum sp. nov. which has common symbiotic genes with sinorhizobium mexicanum. | bacteria from nodules of the legume acaciella angustissima native to the south of mexico were characterized genetically and their nodulation and competitiveness were evaluated. phylogenetic studies derived from rpob gene sequences indicated that a. angustissima is nodulated by sinorhizobium mexicanum, rhizobium tropici, mesorhizobium plurifarium and agrobacterium tumefaciens and by bacteria related to sinorhizobium americanum, sinorhizobium terangae, rhizobium etli and rhizobium gallicum. a new ... | 2009 | 19120461 |
| characterization of a {gamma}-aminobutyric acid transport system of rhizobium leguminosarum bv. viciae 3841. | spontaneous mutants of rhizobium leguminosarum bv. viciae 3841 were isolated that grow faster than the wild type on gamma-aminobutyric acid (gaba) as the sole carbon and nitrogen source. these strains (ru1736 and ru1816) have frameshift mutations (gtsr101 and gtsr102, respectively) in a gntr-type regulator (gtsr) that result in a high rate of constitutive gaba transport. tn5 mutagenesis and quantitative reverse transcription-pcr showed that gstr regulates expression of a large operon (prl100242 ... | 2009 | 19103927 |
| characterization of a {gamma}-aminobutyric acid transport system of rhizobium leguminosarum bv. viciae 3841. | spontaneous mutants of rhizobium leguminosarum bv. viciae 3841 were isolated that grow faster than the wild type on gamma-aminobutyric acid (gaba) as the sole carbon and nitrogen source. these strains (ru1736 and ru1816) have frameshift mutations (gtsr101 and gtsr102, respectively) in a gntr-type regulator (gtsr) that result in a high rate of constitutive gaba transport. tn5 mutagenesis and quantitative reverse transcription-pcr showed that gstr regulates expression of a large operon (prl100242 ... | 2009 | 19103927 |
| the repa_n replicons of gram-positive bacteria: a family of broadly distributed but narrow host range plasmids. | the pheromone-responsive conjugative plasmids of enterococcus faecalis and the multiresistance plasmids psk1 and psk41 of staphylococcus aureus are among the best studied plasmids native to gram-positive bacteria. although these plasmids seem largely restricted to their native hosts, protein sequence comparison of their replication initiator proteins indicates that they are clearly related. homology searches indicate that these replicons are representatives of a large family of plasmids and a fe ... | 2009 | 19100285 |
| multidrug and toxic compound extrusion-type transporters implicated in vacuolar sequestration of nicotine in tobacco roots. | nicotine is a major alkaloid accumulating in the vacuole of tobacco (nicotiana tabacum), but the transporters involved in the vacuolar sequestration are not known. we here report that tobacco genes (ntmate1 and ntmate2) encoding transporters of the multidrug and toxic compound extrusion (mate) family are coordinately regulated with structural genes for nicotine biosynthesis in the root, with respect to spatial expression patterns, regulation by nic regulatory loci, and induction by methyl jasmon ... | 2009 | 19098091 |
| rna silencing is required for arabidopsis defence against verticillium wilt disease. | rna silencing is a conserved mechanism in eukaryotes that plays an important role in various biological processes including regulation of gene expression. rna silencing also plays a role in genome stability and protects plants against invading nucleic acids such as transgenes and viruses. recently, rna silencing has been found to play a role in defence against bacterial plant pathogens in arabidopsis through modulating host defence responses. in this study, it is shown that gene silencing plays ... | 2009 | 19098131 |
| rna silencing is required for arabidopsis defence against verticillium wilt disease. | rna silencing is a conserved mechanism in eukaryotes that plays an important role in various biological processes including regulation of gene expression. rna silencing also plays a role in genome stability and protects plants against invading nucleic acids such as transgenes and viruses. recently, rna silencing has been found to play a role in defence against bacterial plant pathogens in arabidopsis through modulating host defence responses. in this study, it is shown that gene silencing plays ... | 2009 | 19098131 |
| leaf proteome analysis of transgenic plants expressing antiviral antibodies. | the expression of exogenous antibodies in plant is an effective strategy to confer protection against viral infection or to produce molecules with pharmaceutical interest. however, the acceptance of the transgenic technology to obtain self-protecting plants depends on the assessment of their substantial equivalence compared to non-modified crops with an established history of safe use. in fact, the possibility exists that the introduction of transgenes in plants may alter expression of endogenou ... | 2009 | 19099506 |
| overexpression of the chitosanase gene in fusarium solani via agrobacterium tumefaciens-mediated transformation. | to overexpress the chitosanase gene (csn) in f. solani, a vector based on pcambia 1300 was constructed. the csn gene, which is under control of the aspergillus nidulans gpda promoter and a. nidulans trpc terminator, was introduced back into the f. solani genome by agrobacterium tumefaciens-mediated transformation, and the herbicide-resistance gene bar from streptomyces hygroscopicus was used as the selection marker. transformants which showed a significant increase in chitosanase production (app ... | 2009 | 19093150 |
| characterization of 5-aminolevulinate synthase from agrobacterium radiobacter, screening new inhibitors for 5-aminolevulinate dehydratase from escherichia coli and their potential use for high 5-aminolevulinate production. | the hema gene encoding 5-aminolevulinate synthase (alas) from agrobacterium radiobacter zju-0121 showed 92.6% homology with that from a. radiobacter atcc4718 and contained several rare codons. to enhance the expression of this gene, escherichia coli rosetta(de3), which is a rare codon optimizer strain, was used as the host to construct an efficient recombinant strain. and the encoded protein was over-expressed as fusion protein and was purified by affinity purification on ni-nta agarose and by g ... | 2009 | 19095441 |
| agrobacterium tumefaciens-mediated genetic transformation of a recalcitrant grain legume, lentil (lens culinaris medik). | a simple and reproducible agrobacterium-mediated transformation protocol for a recalcitrant legume plant, lentil (lens culinaris m.) is reported. application of wounding treatments and efficiencies of three agrobacterium tumefaciens strains, eha105, c58c1, and kyrt1 were compared for t-dna delivery into lentil cotyledonary node tissues. kyrt1 was found to be on average 2.8-fold more efficient than both eha105 and c58c1 for producing transient beta-glucuronidase (gus) gene (gus) expression on cot ... | 2009 | 19083242 |
| a seldi-tof ms procedure for the detection, quantitation, and preliminary characterization of low-molecular-weight recombinant proteins expressed in transgenic plants. | we describe a seldi-tof ms procedure for the rapid detection and quantitation of low-molecular-weight recombinant proteins expressed in plants. transgenic lines of potato (solanum tuberosum l.) expressing the clinically useful protein bovine aprotinin or the cysteine protease inhibitor corn cystatin ii were generated by agrobacterium tumefaciens-mediated transformation, and then used as test material for the analyses. real-time rt-pcr amplifications and detection of the recombinant proteins by i ... | 2009 | 19086095 |
| a chromosomally located trahijkclmn operon encoding a putative type iv secretion system is involved in the virulence of yersinia ruckeri. | nucleotide sequence analysis of the region surrounding the pivet8 insertion site in yersinia ruckeri 150rivixii, previously selected by in vivo expression technology (ivet), revealed the presence of eight genes (trahijkclmn [hereafter referred to collectively as the tra operon or tra cluster]), which are similar both in sequence and organization to the tra operon cluster found in the virulence-related plasmid padap from serratia entomophila. interestingly, the tra cluster of y. ruckeri is chromo ... | 2009 | 19088314 |
| a chromosomally located trahijkclmn operon encoding a putative type iv secretion system is involved in the virulence of yersinia ruckeri. | nucleotide sequence analysis of the region surrounding the pivet8 insertion site in yersinia ruckeri 150rivixii, previously selected by in vivo expression technology (ivet), revealed the presence of eight genes (trahijkclmn [hereafter referred to collectively as the tra operon or tra cluster]), which are similar both in sequence and organization to the tra operon cluster found in the virulence-related plasmid padap from serratia entomophila. interestingly, the tra cluster of y. ruckeri is chromo ... | 2009 | 19088314 |
| an orphan luxr homolog of sinorhizobium meliloti affects stress adaptation and competition for nodulation. | the sin/expr quorum-sensing system of sinorhizobium meliloti plays an important role in the symbiotic association with its host plant, medicago sativa. the luxr-type response regulators of the sin system include the synthase (sini)-associated sinr and the orphan regulator expr. interestingly, the s. meliloti rm1021 genome codes for four additional putative orphan luxr homologs whose regulatory roles remain to be identified. these response regulators contain the characteristic domains of the luxr ... | 2009 | 19088317 |
| an orphan luxr homolog of sinorhizobium meliloti affects stress adaptation and competition for nodulation. | the sin/expr quorum-sensing system of sinorhizobium meliloti plays an important role in the symbiotic association with its host plant, medicago sativa. the luxr-type response regulators of the sin system include the synthase (sini)-associated sinr and the orphan regulator expr. interestingly, the s. meliloti rm1021 genome codes for four additional putative orphan luxr homologs whose regulatory roles remain to be identified. these response regulators contain the characteristic domains of the luxr ... | 2009 | 19088317 |
| functional mapping of brucella abortus cyclic beta-1,2-glucan synthase: identification of the protein domain required for cyclization. | cyclic beta-1,2-glucans (cbetag) are periplasmic homopolysaccharides that have been shown to play an important role in several symbiotic and pathogenic relationships. cyclic beta-1,2-glucan synthase (cgs), the enzyme responsible for the synthesis of cbetag, is an integral membrane polyfunctional protein that catalyzes the four enzymatic activities (initiation, elongation, phosphorolysis, and cyclization) required for the synthesis of cbetag. recently, we have identified the glycosyltransferase a ... | 2009 | 19074375 |
| functional mapping of brucella abortus cyclic beta-1,2-glucan synthase: identification of the protein domain required for cyclization. | cyclic beta-1,2-glucans (cbetag) are periplasmic homopolysaccharides that have been shown to play an important role in several symbiotic and pathogenic relationships. cyclic beta-1,2-glucan synthase (cgs), the enzyme responsible for the synthesis of cbetag, is an integral membrane polyfunctional protein that catalyzes the four enzymatic activities (initiation, elongation, phosphorolysis, and cyclization) required for the synthesis of cbetag. recently, we have identified the glycosyltransferase a ... | 2009 | 19074375 |
| quorum quenching in agrobacterium tumefaciens: chance or necessity? | 2009 | 19074377 | |
| chromatin organization and radio resistance in the bacterium gemmata obscuriglobus. | the organization of chromatin has a major impact on cellular activities, such as gene expression. for bacteria, it was suggested that the spatial organization of the genetic material correlates with transcriptional levels, implying a specific architecture of the chromosome within the cytoplasm. accordingly, recent technological advances have emphasized the organization of the genetic material within nucleoid structures. gemmata obscuriglobus, a member of the phylum planctomycetes, exhibits a dis ... | 2009 | 19074379 |
| chromatin organization and radio resistance in the bacterium gemmata obscuriglobus. | the organization of chromatin has a major impact on cellular activities, such as gene expression. for bacteria, it was suggested that the spatial organization of the genetic material correlates with transcriptional levels, implying a specific architecture of the chromosome within the cytoplasm. accordingly, recent technological advances have emphasized the organization of the genetic material within nucleoid structures. gemmata obscuriglobus, a member of the phylum planctomycetes, exhibits a dis ... | 2009 | 19074379 |
| genetic diversity and host range of rhizobia nodulating lotus tenuis in typical soils of the salado river basin (argentina). | a total of 103 root nodule isolates were used to estimate the diversity of bacteria nodulating lotus tenuis in typical soils of the salado river basin. a high level of genetic diversity was revealed by repetitive extragenic palindromic pcr, and 77 isolates with unique genomic fingerprints were further differentiated into two clusters, clusters a and b, after 16s rrna restriction fragment length polymorphism analysis. cluster a strains appeared to be related to the genus mesorhizobium, whereas cl ... | 2009 | 19074602 |
| genetic diversity and host range of rhizobia nodulating lotus tenuis in typical soils of the salado river basin (argentina). | a total of 103 root nodule isolates were used to estimate the diversity of bacteria nodulating lotus tenuis in typical soils of the salado river basin. a high level of genetic diversity was revealed by repetitive extragenic palindromic pcr, and 77 isolates with unique genomic fingerprints were further differentiated into two clusters, clusters a and b, after 16s rrna restriction fragment length polymorphism analysis. cluster a strains appeared to be related to the genus mesorhizobium, whereas cl ... | 2009 | 19074602 |
| xanthone biosynthesis in hypericum perforatum cells provides antioxidant and antimicrobial protection upon biotic stress. | xanthone production in hypericum perforatum (hp) suspension cultures in response to elicitation by agrobacterium tumefaciens co-cultivation has been studied. rna blot analyses of hp cells co-cultivated with a. tumefaciens have shown a rapid up-regulation of genes encoding important enzymes of the general phenylpropanoid pathway (pal, phenylalanine ammonia lyase and 4cl, 4-coumarate:coa ligase) and xanthone biosynthesis (bps, benzophenone synthase). analyses of hplc chromatograms of methanolic ex ... | 2009 | 19062051 |
| oryr is a luxr-family protein involved in interkingdom signaling between pathogenic xanthomonas oryzae pv. oryzae and rice. | xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight in rice, contains a regulator that is encoded in the genome, designated oryr, which belongs to the n-acyl homoserine lactone (ahl)-dependent quorum-sensing luxr subfamily of proteins. however, we previously reported that x. oryzae pv. oryzae does not make ahls and does not possess a luxi-family ahl synthase and that the oryr protein is solubilized by a compound present in rice. in this study we obtained further evidence tha ... | 2009 | 19028884 |
| cloning and characterization of uronate dehydrogenases from two pseudomonads and agrobacterium tumefaciens strain c58. | uronate dehydrogenase has been cloned from pseudomonas syringae pv. tomato strain dc3000, pseudomonas putida kt2440, and agrobacterium tumefaciens strain c58. the genes were identified by using a novel complementation assay employing an escherichia coli mutant incapable of consuming glucuronate as the sole carbon source but capable of growth on glucarate. a shotgun library of p. syringae was screened in the mutant e. coli by growing transformed cells on minimal medium containing glucuronic acid. ... | 2009 | 19060141 |
| a 1.1-kilobase region downstream of the bin operon in bacillus sphaericus strain 2362 decreases bin yield and crystal size in strain 2297. | the 2297 strain of bacillus sphaericus produces a crystal of the bin (binary) toxin that is approximately fourfold larger than that of strain 2362, the strain currently used in vectolex, a commercial mosquito larvicide. comparison of the regions downstream from the bin operon in these two strains showed that strain 2362 contained a 1.6-kb region with four orf genes not found in strain 2297. insertion of a 1.1-kb portion of this region from strain 2362 by homologous recombination downstream from ... | 2009 | 19060165 |
| a 1.1-kilobase region downstream of the bin operon in bacillus sphaericus strain 2362 decreases bin yield and crystal size in strain 2297. | the 2297 strain of bacillus sphaericus produces a crystal of the bin (binary) toxin that is approximately fourfold larger than that of strain 2362, the strain currently used in vectolex, a commercial mosquito larvicide. comparison of the regions downstream from the bin operon in these two strains showed that strain 2362 contained a 1.6-kb region with four orf genes not found in strain 2297. insertion of a 1.1-kb portion of this region from strain 2362 by homologous recombination downstream from ... | 2009 | 19060165 |
| secondary metabolites produced by the marine bacterium halobacillus salinus that inhibit quorum sensing-controlled phenotypes in gram-negative bacteria. | certain bacteria use cell-to-cell chemical communication to coordinate community-wide phenotypic expression, including swarming motility, antibiotic biosynthesis, and biofilm production. here we present a marine gram-positive bacterium that secretes secondary metabolites capable of quenching quorum sensing-controlled behaviors in several gram-negative reporter strains. isolate c42, a halobacillus salinus strain obtained from a sea grass sample, inhibits bioluminescence production by vibrio harve ... | 2009 | 19060172 |
| secondary metabolites produced by the marine bacterium halobacillus salinus that inhibit quorum sensing-controlled phenotypes in gram-negative bacteria. | certain bacteria use cell-to-cell chemical communication to coordinate community-wide phenotypic expression, including swarming motility, antibiotic biosynthesis, and biofilm production. here we present a marine gram-positive bacterium that secretes secondary metabolites capable of quenching quorum sensing-controlled behaviors in several gram-negative reporter strains. isolate c42, a halobacillus salinus strain obtained from a sea grass sample, inhibits bioluminescence production by vibrio harve ... | 2009 | 19060172 |
| improved prediction of malaria degradomes by supervised learning with svm and profile kernel. | the spread of drug resistance through malaria parasite populations calls for the development of new therapeutic strategies. however, the seemingly promising genomics-driven target identification paradigm is hampered by the weak annotation coverage. to identify potentially important yet uncharacterized proteins, we apply support vector machines using profile kernels, a supervised discriminative machine learning technique for remote homology detection, as a complement to the traditional alignment ... | 2009 | 19057851 |
| improved prediction of malaria degradomes by supervised learning with svm and profile kernel. | the spread of drug resistance through malaria parasite populations calls for the development of new therapeutic strategies. however, the seemingly promising genomics-driven target identification paradigm is hampered by the weak annotation coverage. to identify potentially important yet uncharacterized proteins, we apply support vector machines using profile kernels, a supervised discriminative machine learning technique for remote homology detection, as a complement to the traditional alignment ... | 2009 | 19057851 |
| development of an activation tagging system for the basidiomycetous medicinal fungus antrodia cinnamomea. | this study describes the development of an efficient and reliable activation tagging system for the medicinal fungus antrodia cinnamomea. for successful agrobacterium tumefaciens-mediated transformation, different parameters were considered. the agrobacterium concentration of 5 x 10(8) cfu ml(-1), 1mm acetosyringone, 25-d-old mycelia at 0.2 g ml(-1), and co-culture period of 6d were found to be the most optimal conditions for enhancing the transformation efficiency. the mitotic stability of tran ... | 2009 | 19059480 |
| agrobacterium virb10 domain requirements for type iv secretion and t pilus biogenesis. | agrobacterium tumefaciens virb10 couples inner membrane (im) atp energy consumption to substrate transfer through the virb/d4 type iv secretion (t4s) channel and also mediates biogenesis of the virb-encoded t pilus. here, we determined the functional importance of virb10 domains denoted as the: (i) n-terminal cytoplasmic region, (ii) transmembrane (tm) alpha-helix, (iii) proline-rich region (prr) and (iv) c-terminal beta-barrel domain. mutations conferring a transfer- and pilus-minus (tra(-), pi ... | 2009 | 19054325 |
| activity and specificity of the bacterial pd-(d/e)xk homing endonuclease i-ssp6803i. | the restriction endonuclease fold [a three-layer alpha-beta sandwich containing variations of the pd-(d/e)xk nuclease motif] has been greatly diversified during evolution, facilitating its use for many biological functions. here we characterize dna binding and cleavage by the pd-(d/e)xk homing endonuclease i-ssp6803i. unlike most restriction endonucleases harboring the same core fold, the specificity profile of this enzyme extends over a long (17 bp) target site. the dna binding and cleavage spe ... | 2009 | 19038269 |
| activity and specificity of the bacterial pd-(d/e)xk homing endonuclease i-ssp6803i. | the restriction endonuclease fold [a three-layer alpha-beta sandwich containing variations of the pd-(d/e)xk nuclease motif] has been greatly diversified during evolution, facilitating its use for many biological functions. here we characterize dna binding and cleavage by the pd-(d/e)xk homing endonuclease i-ssp6803i. unlike most restriction endonucleases harboring the same core fold, the specificity profile of this enzyme extends over a long (17 bp) target site. the dna binding and cleavage spe ... | 2009 | 19038269 |
| the yvri alternative sigma factor is essential for acid stress induction of oxalate decarboxylase in bacillus subtilis. | yvri is a recently identified alternative sigma factor in bacillus subtilis that requires the coactivator yvrha to activate transcription. previously, a strain engineered to overproduce yvri was found to overproduce oxalate decarboxylase (oxdc), and further analysis identified three yvri-activated promoters preceding the yvri-yvrha, yvrj, and oxdc-yvrl operons. independently, proteome analyses identified oxdc as a highly abundant, cell wall-associated protein that accumulated under acidic growth ... | 2009 | 19047353 |
| the yvri alternative sigma factor is essential for acid stress induction of oxalate decarboxylase in bacillus subtilis. | yvri is a recently identified alternative sigma factor in bacillus subtilis that requires the coactivator yvrha to activate transcription. previously, a strain engineered to overproduce yvri was found to overproduce oxalate decarboxylase (oxdc), and further analysis identified three yvri-activated promoters preceding the yvri-yvrha, yvrj, and oxdc-yvrl operons. independently, proteome analyses identified oxdc as a highly abundant, cell wall-associated protein that accumulated under acidic growth ... | 2009 | 19047353 |
| characterization of serine proteinase expression in agaricus bisporus and coprinopsis cinerea by using green fluorescent protein and the a. bisporus spr1 promoter. | the agaricus bisporus serine proteinase 1 (spr1) appears to be significant in both mycelial nutrition and senescence of the fruiting body. we report on the construction of an spr promoter::green fluorescent protein (gfp) fusion cassette, pgreen_hph1_spr_gfp, for the investigation of temporal and developmental expression of spr1 in homobasidiomycetes and to determine how expression is linked to physiological and environmental stimuli. monitoring of a. bisporus pgreen_hph1_spr_gfp transformants on ... | 2009 | 19047386 |
| characterization of serine proteinase expression in agaricus bisporus and coprinopsis cinerea by using green fluorescent protein and the a. bisporus spr1 promoter. | the agaricus bisporus serine proteinase 1 (spr1) appears to be significant in both mycelial nutrition and senescence of the fruiting body. we report on the construction of an spr promoter::green fluorescent protein (gfp) fusion cassette, pgreen_hph1_spr_gfp, for the investigation of temporal and developmental expression of spr1 in homobasidiomycetes and to determine how expression is linked to physiological and environmental stimuli. monitoring of a. bisporus pgreen_hph1_spr_gfp transformants on ... | 2009 | 19047386 |
| metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to zn accumulation in the metal hyperaccumulator, thlaspi caerulescens. | to study the role of metallothioneins (mts) in zn accumulation, the expression of tcmt2a, tcmt2b, and tcmt3 was analysed in three accessions and 15 f(3) families of two inter-accession crosses of the cd/zn hyperaccumulator thlaspi caerulescens, with different degrees of zn accumulation. the highest expression levels were found in the shoots of a superior metal-accumulating calamine accession from st laurent le minier, with >10-fold tcmt3 expression compared with another calamine accession and a ... | 2009 | 19033549 |
| metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to zn accumulation in the metal hyperaccumulator, thlaspi caerulescens. | to study the role of metallothioneins (mts) in zn accumulation, the expression of tcmt2a, tcmt2b, and tcmt3 was analysed in three accessions and 15 f(3) families of two inter-accession crosses of the cd/zn hyperaccumulator thlaspi caerulescens, with different degrees of zn accumulation. the highest expression levels were found in the shoots of a superior metal-accumulating calamine accession from st laurent le minier, with >10-fold tcmt3 expression compared with another calamine accession and a ... | 2009 | 19033549 |
| athma3, a p1b-atpase allowing cd/zn/co/pb vacuolar storage in arabidopsis. | the arabidopsis (arabidopsis thaliana) heavy metal associated3 (athma3) protein belongs to the p1b-2 subgroup of the p-type atpase family, which is involved in heavy metal transport. in a previous study, we have shown, using heterologous expression in the yeast saccharomyces cerevisiae, that in the presence of toxic metals, athma3 was able to phenotypically complement the cadmium/lead (cd/pb)-hypersensitive strain ycf1 but not the zinc (zn)-hypersensitive strain zrc1. in this study, we demonstra ... | 2009 | 19036834 |
| mutations in the arabidopsis peroxisomal abc transporter comatose allow differentiation between multiple functions in planta: insights from an allelic series. | comatose (cts), the arabidopsis homologue of human adrenoleukodystrophy protein (aldp), is required for import of substrates for peroxisomal beta-oxidation. a new allelic series and a homology model based on the bacterial abc transporter, sav1866, provide novel insights into structure-function relations of abc subfamily d proteins. in contrast to aldp, where the majority of mutations result in protein absence from the peroxisomal membrane, all cts mutants produced stable protein. mutation of con ... | 2009 | 19019987 |
| production of selectable marker-free transgenic tobacco plants using a non-selection approach: chimerism or escape, transgene inheritance, and efficiency. | public concern and metabolic drain were the main driving forces for the development of a selectable marker-free transformation system. we demonstrated here the production of transgenic tobacco plants using a non-selection approach by agrobacterium tumefaciens-mediated transformation. a. tumefaciens-infected leaf explants were allowed to produce shoots on a shoot induction medium (sim) containing no selective compounds. up to 35.1% of the a. tumefaciens-infected leaf explants produced histochemic ... | 2009 | 19018535 |
| metagenome-derived clones encoding two novel lactonase family proteins involved in biofilm inhibition in pseudomonas aeruginosa. | here we report the isolation and characterization of three metagenome-derived clones that interfere with bacterial quorum sensing and degrade n-(3-oxooctanoyl)-l-homoserine lactone (3-oxo-c(8)-hsl). by using a trai-lacz gene fusion, the metagenome-derived clones were identified from a soil dna library and analyzed. the open reading frames linked to the 3-oxo-c(8)-hsl-degrading activities were designated bpib01, bpib04, and bpib07. while the bpib07 protein was similar to a known lactonase, no sig ... | 2009 | 18997026 |