Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| mutation of ser172 in yeast β tubulin induces defects in microtubule dynamics and cell division. | ser172 of β tubulin is an important residue that is mutated in a human brain disease and phosphorylated by the cyclin-dependent kinase cdk1 in mammalian cells. to examine the role of this residue, we used the yeast s. cerevisiae as a model and produced two different mutations (s172a and s172e) of the conserved ser172 in the yeast β tubulin tub2p. the two mutants showed impaired cell growth on benomyl-containing medium and at cold temperatures, altered microtubule (mt) dynamics, and altered nucle ... | 2010 | 21042413 |
| global protein interactome exploration through mining genome-scale data in arabidopsis thaliana. | many essential cellular processes, such as cellular metabolism, transport, cellular metabolism and most regulatory mechanisms, rely on physical interactions between proteins. genome-wide protein interactome networks of yeast, human and several other animal organisms have already been established, but this kind of network reminds to be established in the field of plant. | 2010 | 21047383 |
| nucleosome structure incorporated histone acetylation site prediction in arabidopsis thaliana. | acetylation is a crucial post-translational modification for histones, and plays a key role in gene expression regulation. due to limited data and lack of a clear acetylation consensus sequence, a few researches have focused on prediction of lysine acetylation sites. several systematic prediction studies have been conducted for human and yeast, but less for arabidopsis thaliana. | 2010 | 21047388 |
| the crystal structure of the ubiquitin-like (ubl) domain of human homologue a of rad23 (hhr23a) protein. | the human homologue of the yeast rad23 protein, hhr23a, plays dual roles in dna repair as well as in translocating polyubiquitinated proteins to the proteasome. we determined the three-dimensional structure of its ubiquitin-like (ubl) domain by x-ray crystallography. it has the same overall structure and fold characteristics as ubiquitin and other members of the ubl domain family, with overall root mean square deviations in cα positions in the range of 1.0-1.3 å. there are local differences in t ... | 2010 | 21047872 |
| genetic analysis of b55alpha/cdc55 protein phosphatase 2a subunits: association with the adenovirus e4orf4 protein. | the human adenovirus e4orf4 protein is toxic in both human tumor cells and saccharomyces cerevisiae. previous studies indicated that most of this toxicity is dependent on an interaction of e4orf4 protein with the b55 class of regulatory subunits of protein phosphatase 2a (pp2a) and in yeast with the b55 homolog cdc55. we have found previously that e4orf4 inhibits pp2a activity against at least some substrates. in an attempt to understand the mechanism of this inhibition, we used a genetic approa ... | 2010 | 21047956 |
| epithelial cell secretions from the human female reproductive tract inhibit sexually transmitted pathogens and candida albicans but not lactobacillus. | female reproductive tract (frt) epithelial cells protect against potential pathogens and sexually transmitted infections. the purpose of this study was to determine if epithelial cells from the upper frt secrete antimicrobials that inhibit reproductive tract pathogens that threaten women's health. apical secretions from primary cultures of fallopian tube, uterine, cervical, and ectocervical epithelial cells were incubated with neisseria gonorrhoeae, candida albicans (yeast and hyphal forms), hum ... | 2010 | 21048705 |
| a protein inventory of human ribosome biogenesis reveals an essential function of exportin 5 in 60s subunit export. | the assembly of ribosomal subunits in eukaryotes is a complex, multistep process so far mostly studied in yeast. in s. cerevisiae, more than 200 factors including ribosomal proteins and trans-acting factors are required for the ordered assembly of 40s and 60s ribosomal subunits. to date, only few human homologs of these yeast ribosome synthesis factors have been characterized. here, we used a systematic rna interference (rnai) approach to analyze the contribution of 464 candidate factors to ribo ... | 2010 | 21048991 |
| repairtoire--a database of dna repair pathways. | repairtoire is the first comprehensive database resource for systems biology of dna damage and repair. the database collects and organizes the following types of information: (i) dna damage linked to environmental mutagenic and cytotoxic agents, (ii) pathways comprising individual processes and enzymatic reactions involved in the removal of damage, (iii) proteins participating in dna repair and (iv) diseases correlated with mutations in genes encoding dna repair proteins. repairtoire provides al ... | 2010 | 21051355 |
| the mms22l-tonsl complex mediates recovery from replication stress and homologous recombination. | genome integrity is jeopardized each time dna replication forks stall or collapse. here we report the identification of a complex composed of mms22l (c6orf167) and tonsl (nfkbil2) that participates in the recovery from replication stress. mms22l and tonsl are homologous to yeast mms22 and plant tonsoku/brushy1, respectively. mms22l-tonsl accumulates at regions of ssdna associated with distressed replication forks or at processed dna breaks, and its depletion results in high levels of endogenous ... | 2010 | 21055983 |
| identification of the mms22l-tonsl complex that promotes homologous recombination. | budding yeast mms22 is required for homologous recombination (hr)-mediated repair of stalled or broken dna replication forks. here we identify a human mms22-like protein (mms22l) and an mms22l-interacting protein, nfκbil2/tonsl. depletion of mms22l or tonsl from human cells causes a high level of double-strand breaks (dsbs) during dna replication. both proteins accumulate at stressed replication forks, and depletion of mms22l or tonsl from cells causes hypersensitivity to agents that cause s pha ... | 2010 | 21055984 |
| regulation of m₃ muscarinic receptor expression and function by transmembrane protein 147. | the m₃ muscarinic acetylcholine receptor (m3r) regulates many fundamental physiological functions. to identify novel m3r-interacting proteins, we used a recently developed yeast two-hybrid screen (split ubiquitin method) to detect interactions among membrane proteins. this screen led to the identification of many novel m3r-associated proteins, including the putative membrane protein transmembrane protein 147 (tmem147). the amino acid sequence of tmem147 is highly conserved among mammals, but its ... | 2010 | 21056967 |
| role of host glycosphingolipids on paracoccidioides brasiliensis adhesion. | binding of yeast forms to human lung fibroblast cultures was analyzed, aiming to better understand the initial steps of paracoccidioides brasiliensis infection in humans. a significant p. brasiliensis adhesion was observed either to fibroblasts or to their triton x-100 insoluble fraction, which contains extracellular matrix and membrane microdomains enriched in glycosphingolipids. since human lung fibroblasts express at cell-surface gangliosides, such as gm1, gm2, and gm3, the role of these glyc ... | 2010 | 21057877 |
| members of the pmp protein family of chlamydia pneumoniae mediate adhesion to human cells via short repetitive peptide motifs. | chlamydiae sp. are obligate intracellular pathogens that cause a variety of diseases in humans. adhesion of the infectious elementary body to the eukaryotic host cell is a pivotal step in chlamydial pathogenesis. here we describe the characterization of members of the polymorphic membrane protein family (pmp), the largest protein family (with up to 21 members) unique to chlamydiaceae. we show that yeast cells displaying pmp6, pmp20 or pmp21 on their surfaces, or beads coated with the recombinant ... | 2010 | 21062373 |
| the unusual ubz domain of saccharomyces cerevisiae polymerase η. | recent research has revealed the presence of ubiquitin-binding domains in the y family polymerases. the ubiquitin-binding zinc finger (ubz) domain of human polymerase η is vital for its regulation, localization, and function. here, we elucidate structural and functional features of the non-canonical ubz motif of saccharomyces cerevisiae pol η. characterization of pol η mutants confirms the importance of the ubz motif and implies that its function is independent of zinc binding. intriguingly, we ... | 2010 | 20837403 |
| high resolution models of transcription factor-dna affinities improve in vitro and in vivo binding predictions. | accurately modeling the dna sequence preferences of transcription factors (tfs), and using these models to predict in vivo genomic binding sites for tfs, are key pieces in deciphering the regulatory code. these efforts have been frustrated by the limited availability and accuracy of tf binding site motifs, usually represented as position-specific scoring matrices (pssms), which may match large numbers of sites and produce an unreliable list of target genes. recently, protein binding microarray ( ... | 2010 | 20838582 |
| potential therapeutic targets for neurodegenerative diseases: lessons learned from calorie restriction. | it is well known that calorie restriction (cr) can retard the aging process in organisms ranging from yeast to non-human primates, and delay the onset of numerous age-related diseases including neurodegenerative disorders. translation of the knowledge gained from cr research in animal models to disease prevention strategies in humans should provide therapeutic approaches for these diseases. signaling pathways induced by cr are therefore potentially new therapeutic targets for neurodegenerative d ... | 2010 | 20840070 |
| atp is required for interactions between uap56 and two conserved mrna export proteins, aly and cip29, to assemble the trex complex. | the conserved trex mrna export complex is known to contain uap56, aly, tex1, and the tho complex. here, we carried out proteomic analysis of immunopurified human trex complex and identified the protein cip29 as the only new component with a clear yeast relative (known as tho1). tho1 is known to function in mrna export, and we provide evidence that cip29 likewise functions in this process. like the known trex components, a portion of cip29 localizes in nuclear speckle domains, and its efficient r ... | 2010 | 20844015 |
| barcode technology in yeast: application to pharmacogenomics. | the common baker's yeast, saccharomyces cerevisiae, is one of the oldest domesticated organisms known, and has been exploited by our ancestors in several different applications, particularly in food and fermentations industries and in bioconversion and biodegradation processes. over the years, yeast has become an excellent experimental model for biological and medical studies, thanks to its genetic tractability, well-known physiology and fast-doubling cycle. in the last decade, the advances in g ... | 2010 | 20846145 |
| evidence mining and novelty assessment of protein-protein interactions with the consensuspathdb plugin for cytoscape. | protein-protein interaction detection methods are applied on a daily basis by molecular biologists worldwide. after generating a set of potential interactions, biologists face the problem of highlighting the ones that are novel and collecting evidence with respect to literature and annotation. this task can be as tedious as searching for every predicted interaction in several interaction data repositories, or manually screening the scientific literature. to facilitate the task of evidence mining ... | 2010 | 20847220 |
| autolytic activity of human calpain 7 is enhanced by escrt-iii-related protein ist1 through mit-mim interaction. | calpain 7, a mammalian ortholog of yeast cpl1/rim13 and fungal palb, is an atypical calpain that lacks a penta-ef-hand domain. previously, we reported that a region containing a tandem repeat of microtubule-interacting and transport (mit) domains in calpain 7 interacts with a subset of endosomal sorting complex required for transport (escrt)-iii-related proteins, suggesting involvement of calpain 7 in the escrt system. although yeast and fungal calpains are thought to be involved in alkaline ada ... | 2010 | 20849418 |
| internalizing cancer antibodies from phage libraries selected on tumor cells and yeast-displayed tumor antigens. | a number of approaches have been utilized to generate antibodies to cancer cell surface receptors that can be used as potential therapeutics. a number of these therapeutic approaches, including antibody-drug conjugates, immunotoxins, and targeted nucleic acid delivery, require antibodies that not only bind receptor but also undergo internalization into the cell upon binding. we previously reported on the ability to generate cancer cell binding and internalizing antibodies directly from human pha ... | 2010 | 20851130 |
| metabolism of sesamin by cytochrome p450 in human liver microsomes. | metabolism of sesamin by cytochrome p450 (p450) was examined using yeast expression system and human liver microsomes. saccharomyces cerevisiae cells expressing each of human p450 isoforms (cyp1a1, 1a2, 2a6, 2b6, 2c8, 2c9, 2c18, 2c19, 2d6, 2e1, and 3a4) were cultivated with sesamin, and monocatechol metabolite was observed in most of p450s. kinetic analysis using the microsomal fractions of the recombinant s. cerevisiae cells revealed that cyp2c19 had the largest k(cat)/k(m) value. based on the ... | 2010 | 20851877 |
| production of heterologous glycoproteins by a glycosylation-defective alg3och1 mutant of schizosaccharomyces pombe. | the early stages of n-linked glycosylation are highly conserved between fungal and mammalian cells. such n-linked oligosaccharides are synthesized through the ordered assembly of a dolichyl pyrophosphate (dol-pp)-linked glc(3)man(9)glcnac(2) structure by the sequential actions of several glycosyltransferases located in the endoplasmic reticulum (er). of the glycosyltransferase genes, saccharomyces cerevisiae alg3 has been identified to encode the dol-p-man:man(5)glcnac(2)-pp-dol α1,3-mannosyltra ... | 2010 | 20854854 |
| methylviewer: computational analysis and editing for bisulfite sequencing and methyltransferase accessibility protocol for individual templates (mapit) projects. | bisulfite sequencing is a widely-used technique for examining cytosine dna methylation at nucleotide resolution along single dna strands. probing with cytosine dna methyltransferases followed by bisulfite sequencing (mapit) is an effective technique for mapping protein-dna interactions. here, mapit methylation footprinting with m.cvipi, a gc methyltransferase we previously cloned and characterized, was used to probe hmlh1 chromatin in hct116 and rko colorectal cancer cells. because m.cvipi-probe ... | 2010 | 20959287 |
| ph-dependent localization of btn1p in the yeast model for batten disease. | btn1p the yeast homolog of human cln3, which is associated with juvenile batten disease has been implicated in several cellular pathways. yeast cells lacking btn1 are unable to couple atp hydrolysis and proton pumping activities by the vacuolar atpase (v-atpase). in this work, we demonstrate that changes in extracellular ph result in altered transcription of btn1, as well as a change in the glycosylation state and localization of btn1p. at high ph, btn1p expression was increased and the protein ... | 2010 | 20959629 |
| identification of binary interactions between human cytomegalovirus virion proteins. | human cytomegalovirus (hcmv) virions are composed of a dna-containing nucleocapsid surrounded by a tegument layer and host-derived lipid envelope studded with virally encoded glycoproteins. these complex virions are estimated to be composed of more than 50 viral proteins. assembly of hcmv virions is poorly understood, especially with respect to acquisition of the tegument; however, it is thought to involve the stepwise addition of virion components through protein-protein interactions. we sought ... | 2010 | 20962080 |
| yeast-elicited cross-reactive antibodies to hiv env glycans efficiently neutralize virions expressing exclusively high-mannose n-linked glycans. | the hiv envelope (env) protein uses a dense coat of glycans to mask conserved domains and evade host humoral immune responses. the broadly neutralizing antibody 2g12, which binds a specific cluster of high-mannose glycans on hiv env, shows that the glycan shield can also serve as a target for neutralizing antibodies. we have described a triple mutant saccharomyces cerevisiae strain that expresses high-mannose glycoproteins that bind to 2g12. when used to immunize rabbits, this yeast elicits anti ... | 2010 | 20962094 |
| glutathione biosynthesis in the yeast pathogens candida glabrata and candida albicans: essential in c. glabrata, and essential for virulence in c. albicans. | redox pathways play a key role in pathogenesis. glutathione, a central molecule in redox homeostasis in yeasts, is an essential metabolite, but its requirements can be met either from endogenous biosynthesis or from the extracellular milieu. in this report we have examined the importance of glutathione biosynthesis in two major human opportunistic fungal pathogens, candida albicans and candida glabrata. as the genome sequence of c. glabrata had suggested the absence of glutathione transporters, ... | 2010 | 20966090 |
| chlorophenol stress affects aromatic amino acid biosynthesis-a genome-wide study. | chlorophenols are a class of chemicals commonly used in preservatives, disinfectants, algaecides, herbicides and pesticides. however, there is a growing evidence that these compounds are a threat to human health. this is alarming as many chlorophenols are common pollutants found in the global environment at potentially biohazardous levels. despite chlorophenols being abundant, widely used and poisonous, we know relatively little about their mechanism of toxicity in eukaryotes. thus, we performed ... | 2010 | 20967895 |
| production of tranilast [n-(3',4'-dimethoxycinnamoyl)-anthranilic acid] and its analogs in yeast saccharomyces cerevisiae. | biological synthesis of therapeutic drugs beneficial for human health using microbes offers an alternative production strategy to the methods that are commonly employed such as direct extraction from source organisms or chemical synthesis. in this study, we evaluated the potential for yeast (saccharomyces cerevisiae) to be used as a catalyst for the synthesis of tranilast and various tranilast analogs (cinnamoyl anthranilates). several studies have demonstrated that these phenolic amides have an ... | 2010 | 20972784 |
| sugar-fermenting yeast as an organic source of carbon dioxide to attract the malaria mosquito anopheles gambiae. | carbon dioxide (co2) plays an important role in the host-seeking process of opportunistic, zoophilic and anthropophilic mosquito species and is, therefore, commonly added to mosquito sampling tools. the african malaria vector anopheles gambiae sensu stricto is attracted to human volatiles augmented by co2. this study investigated whether co2, usually supplied from gas cylinders acquired from commercial industry, could be replaced by co2 derived from fermenting yeast (yeast-produced co2). | 2010 | 20973963 |
| insights into cdc13 dependent telomere length regulation. | cdc13 is a single stranded telomere binding protein that specifically localizes to the telomere ends of budding yeasts and is essential for cell viability. it caps the ends of chromosomes thus preventing chromosome end-to-end fusions and exonucleolytic degradation, events that could lead to genomic instability and senescence, the hallmark of aging. cdc13 is also involved in telomere length regulation by recruiting or preventing access of telomerase to the telomeric overhang. recruitment of telom ... | 2010 | 20975208 |
| characterization of nicotinamidases: steady state kinetic parameters, classwide inhibition by nicotinaldehydes, and catalytic mechanism. | nicotinamidases are metabolic enzymes that hydrolyze nicotinamide to nicotinic acid. these enzymes are widely distributed across biology, with examples found encoded in the genomes of mycobacteria, archaea, eubacteria, protozoa, yeast, and invertebrates, but there are none found in mammals. although recent structural work has improved our understanding of these enzymes, their catalytic mechanism is still not well understood. recent data show that nicotinamidases are required for the growth and v ... | 2010 | 20979384 |
| rescue of hiv-1 release by targeting widely divergent nedd4-type ubiquitin ligases and isolated catalytic hect domains to gag. | retroviruses engage the escrt pathway through late assembly (l) domains in gag to promote virus release. hiv-1 uses a ptap motif as its primary l domain, which interacts with the escrt-i component tsg101. in contrast, certain other retroviruses primarily use ppxy-type l domains, which constitute ligands for nedd4-type ubiquitin ligases. surprisingly, although hiv-1 gag lacks ppxy motifs, the release of hiv-1 l domain mutants is potently enhanced by ectopic nedd4-2s, a native isoform with a natur ... | 2010 | 20862313 |
| [screening of candidate proteins interacting with foxp3δ2 by yeast two-hybrid system]. | to screen foxp3delta2-interacting proteins by yeast two-hybria. | 2010 | 20862797 |
| a simple definition of structural regions in proteins and its use in analyzing interface evolution. | analysis of proteins commonly requires the partition of their structure into regions such as the surface, interior, or interface. despite the frequent use of such categorization, no consensus definition seems to exist. this study thus aims at providing a definition that is general, is simple to implement, and yields new biological insights. this analysis relies on 397, 196, and 701 protein structures from escherichia coli, saccharomyces cerevisiae, and homo sapiens, respectively, and the conclus ... | 2010 | 20868694 |
| coxiella burnetii expresses a functional δ24 sterol reductase. | coxiella burnetii, the etiological agent of human q fever, occupies a unique niche inside the host cell, where it replicates in a modified acidic phagolysosome or parasitophorous vacuole (pv). the pv membrane is cholesterol-rich, and inhibition of host cholesterol metabolism negatively impacts pv biogenesis and pathogen replication. the precise source(s) of pv membrane cholesterol is unknown, as is whether the bacterium actively diverts and/or modifies host cell cholesterol or sterol precursors. ... | 2010 | 20870767 |
| selenium: a double-edged sword for defense and offence in cancer. | selenium (se) is an essential dietary component for animals including humans and is regarded as a protective agent against cancer. although the mode of anticancer action of se is not fully understood yet, several mechanisms, such as antioxidant protection by selenoenzymes, specific inhibition of tumor cell growth by se metabolites, modulation of cell cycle and apoptosis, and effect on dna repair have all been proposed. despite the unsupported results of the last select trial, the cancer-preventi ... | 2010 | 20871980 |
| characterization of hnek6 interactome reveals an important role for its short n-terminal domain and colocalization with proteins at the centrosome. | physical protein-protein interactions are fundamental to all biological processes and are organized in complex networks. one branch of the kinome network is the evolutionarily conserved nima-related serine/threonine kinases (neks). most of the 11 mammalian neks studied so far are related to cell cycle regulation, and due to association with diverse human pathologies, neks are promising chemotherapeutic targets. human nek6 was associated to carcinogenesis, but its interacting partners and signali ... | 2010 | 20873783 |
| adiponectin receptor binding proteins--recent advances in elucidating adiponectin signalling pathways. | adiponectin whose systemic levels are reduced in obesity-related diseases ameliorates insulin sensitivity and regulates biological processes like apoptosis, proliferation, migration and inflammation. adiponectin binds to adiponectin receptors, adipor1 and adipor2, which are ubiquitously expressed. clathrin-dependent endocytosis of adipor1 and adiponectin has been demonstrated to modulate adiponectin bioactivity. recently, appl1 has been identified as an adipor1 and adipor2 binding protein. furth ... | 2010 | 20875820 |
| a 32-kilodalton hydrolase plays an important role in paracoccidioides brasiliensis adherence to host cells and influences pathogenicity. | one of the most crucial events during infection with the dimorphic fungus paracoccidioides brasiliensis is adhesion to pulmonary epithelial cells, a pivotal step in the establishment of disease. in this study, we have evaluated the relevance of a 32-kda protein, a putative adhesion member of the haloacid dehalogenase (had) superfamily of hydrolases, in the virulence of this fungus. protein sequence analyses have supported the inclusion of pbhad32p as a hydrolase and have revealed a conserved pro ... | 2010 | 20876288 |
| quantitative expression profiling of g-protein-coupled receptors (gpcrs) in metastatic melanoma: the constitutively active orphan gpcr gpr18 as novel drug target. | g-protein-coupled receptors (gpcrs) have been implicated in the tumorigenesis and metastasis of human cancers and are considered amongst the most desirable targets for drug development. utilizing a robust quantitative pcr array, we quantified expression of 94 human gpcrs, including 75 orphan gpcrs and 19 chemokine receptors, and 36 chemokine ligands, in 40 melanoma metastases from different individuals and benign nevi. inter-metastatic site comparison revealed that orphan gpr174 and ccl28 are st ... | 2010 | 20880198 |
| allicin disrupts the cell's electrochemical potential and induces apoptosis in yeast. | the volatile substance allicin gives crushed garlic (allium sativum) its characteristic odor and is a pro-oxidant that undergoes thiol-disulfide exchange reactions with -sh groups in proteins and glutathione. the antimicrobial activity of allicin is suspected to be due to the oxidative inactivation of essential thiol-containing enzymes. we investigated the hypothesis that at threshold inhibitory levels allicin can shunt yeast cells into apoptosis by altering their overall redox status. yeast cel ... | 2010 | 20883774 |
| finding the "dark matter" in human and yeast protein network prediction and modelling. | accurate modelling of biological systems requires a deeper and more complete knowledge about the molecular components and their functional associations than we currently have. traditionally, new knowledge on protein associations generated by experiments has played a central role in systems modelling, in contrast to generally less trusted bio-computational predictions. however, we will not achieve realistic modelling of complex molecular systems if the current experimental designs lead to biased ... | 2010 | 20885791 |
| highly specific antibodies for co-detection of human choline kinase α1 and α2 isoforms. | choline kinase is the first enzyme in the cdp-choline pathway that synthesizes phosphatidylcholine, the major phospholipid in eukaryotic cell membranes. in humans, choline kinase exists as three isoforms (ckα1, α2, and β). specific inhibition of ckα has been reported to selectively kill tumoral cells. monoclonal and polyclonal antibodies against ckα used in previous studies to detect the level of this isozyme in different cellular or biochemical contexts were able to detect either the α1 or the ... | 2010 | 20886003 |
| confronting cellular heterogeneity in studies of protein metabolism and homeostasis in aging research. | in this chapter we review the different technologies that can be applied in the analysis of protein homeostasis and metabolism in aging research. special focus will be on technologies with a potential to circumvent the problems associated with cell heterogeneity in biomarker discovery. often studies aimed at increasing our understanding of cellular senescence take advantage of model systems. this can be in the form of cell culture, where specific celllines are cultivated, thus undergoing cellula ... | 2010 | 20886767 |
| identification of novel species-selective agonists of the g-protein-coupled receptor gpr35 that promote recruitment of β-arrestin-2 and activate gα13. | the poorly characterized g-protein-coupled receptor gpr35 has been suggested as a potential exploratory target for the treatment of both metabolic disorders and hypertension. it has also been indicated to play an important role in immune modulation. a major impediment to validation of these concepts and further study of the role of this receptor has been a paucity of pharmacological tools that interact with gpr35. using a receptor-β-arrestin-2 interaction assay with both human and rat orthologue ... | 2010 | 20919992 |
| the beta1 subunit of the na,k-atpase pump interacts with megalencephalic leucoencephalopathy with subcortical cysts protein 1 (mlc1) in brain astrocytes: new insights into mlc pathogenesis. | megalencephalic leucoencephalopathy with subcortical cysts (mlc) is a rare congenital leucodystrophy caused by mutations in mlc1, a membrane protein of unknown function. mlc1 expression in astrocyte end-feet contacting blood vessels and meninges, along with brain swelling, fluid cysts and myelin vacuolation observed in mlc patients, suggests a possible role for mlc1 in the regulation of fluid and ion homeostasis and cellular volume changes. to identify mlc1 direct interactors and dissect the mol ... | 2010 | 20926452 |
| mitochondrial dna replication and disease: insights from dna polymerase γ mutations. | dna polymerase γ (pol γ), encoded by polg, is responsible for replicating human mitochondrial dna. about 150 mutations in the human polg have been identified in patients with mitochondrial diseases such as alpers syndrome, progressive external ophthalmoplegia, and ataxia-neuropathy syndromes. because many of the mutations are described in single citations with no genotypic family history, it is important to ascertain which mutations cause or contribute to mitochondrial disease. the vast majority ... | 2010 | 20927567 |
| asc1, a wd-repeat protein, is required for hyphal development and virulence in candida albicans. | candida albicans is a human pathogenic fungus which can undergo a morphological transition from yeast to hyphae in response to a variety of environmental stimuli. we analyzed a c. albicans asc1 (absence of growth suppressor of cyp1) protein which is entirely composed of seven repeats of the wd domain, and is conserved from fungi to metazoan. deleting the asc1 in c. albicans led to a profound defect in hyphal development under hypha-inducing conditions examined. furthermore, deletion of the asc1 ... | 2010 | 20929924 |
| alternative oligomeric states of the yeast rvb1/rvb2 complex induced by histidine tags. | rvb1 and rvb2 are essential aaa(+) (atpases associated with diverse cellular activities) helicases, which are important components of critical complexes such as chromatin remodeling and telomerase complexes. the oligomeric state of the rvb proteins has been controversial. independent studies from several groups have described the yeast and human rvb1/rvb2 complex both as a single and as a double hexameric ring complex. we found that histidine-tagged constructs of yeast rvb proteins employed in s ... | 2010 | 20934430 |
| replication past the γ-radiation-induced guanine-thymine cross-link g[8,5-me]t by human and yeast dna polymerase η. | γ-radiation-induced intrastrand guanine-thymine cross-link, g[8,5-me]t, hinders replication in vitro and is mutagenic in mammalian cells. herein we report in vitro translesion synthesis of g[8,5-me]t by human and yeast dna polymerase η (hpol η and ypol η). damp misincorporation opposite the cross-linked g by ypol η was preferred over correct incorporation of dcmp, but further extension was 100-fold less efficient for g(∗):a compared to g(∗):c. for hpol η, both incorporation and extension were mo ... | 2010 | 20936176 |
| av119, a natural sugar from avocado gratissima, modulates the lps-induced proinflammatory response in human keratinocytes. | keratinocytes play an active role in innate immune responses by secreting a variety of cytokines and chemokines. the release of critical proinflammatory cytokines, which are necessary to activate the immune response, is induced by the stimulation of toll-like receptors (tlrs) by molecules present on pathogenic micro-organisms such as lipopolysaccharide (lps). av119, a patented blend of avocado sugars, induced the aggregation of malassezia furfur, a dimorphic, lipid-dependent yeast that is part o ... | 2010 | 20936426 |
| a human map kinase interactome. | mitogen-activated protein kinase (mapk) pathways form the backbone of signal transduction in the mammalian cell. here we applied a systematic experimental and computational approach to map 2,269 interactions between human mapk-related proteins and other cellular machinery and to assemble these data into functional modules. multiple lines of evidence including conservation with yeast supported a core network of 641 interactions. using small interfering rna knockdowns, we observed that approximate ... | 2010 | 20936779 |
| adaptation to diverse nitrogen-limited environments by deletion or extrachromosomal element formation of the gap1 locus. | to study adaptive evolution in defined environments, we performed evolution experiments with saccharomyces cerevisiae (yeast) in nitrogen-limited chemostat cultures. we used dna microarrays to identify copy-number variation associated with adaptation and observed frequent amplifications and deletions at the gap1 locus. gap1 encodes the general amino acid permease, which transports amino acids across the plasma membrane. we identified a self-propagating extrachromosomal circular dna molecule that ... | 2010 | 20937885 |
| elovl1 production of c24 acyl-coas is linked to c24 sphingolipid synthesis. | very long-chain fatty acids (vlcfas) exert a variety of cellular functions and are associated with numerous diseases. however, the precise pathway behind their elongation has remained elusive. moreover, few regulatory mechanisms for vlcfas synthesis have been identified. elongases catalyze the first of four steps in the vlcfa elongation cycle; mammals have seven elongases (elovl1-7). in the present study, we determined the precise substrate specificities of all the elovls by in vitro analyses. p ... | 2010 | 20937905 |
| biochemical, cell biological, and genetic assays to analyze amyloid and prion aggregation in yeast. | protein aggregates are associated with a variety of debilitating human diseases, but they can have functional roles as well. both pathological and nonpathological protein aggregates display tremendous diversity, with substantial differences in aggregate size, morphology, and structure. among the different aggregation types, amyloids are particularly remarkable, because of their high degree of order and their ability to form self-perpetuating conformational states. amyloids form the structural ba ... | 2010 | 20946833 |
| applying genetics and molecular biology to the study of the human pathogen cryptococcus neoformans. | the basidiomycete yeast crytococcus neoformans is a prominent human pathogen. it primarily infects immunocompromised individuals producing a meningoencephalitis that is lethal if untreated. recent advances in its genetics and molecular biology have made it a model system for understanding both the basidiomycota phylum and mechanisms of fungal pathogenesis. the relative ease of experimental manipulation coupled with the development of murine models for human disease allow for powerful studies in ... | 2010 | 20946836 |
| identification of three tomato flower and fruit mads-box proteins with a putative histone deacetylase binding domain. | mads-box transcription factors play crucial roles in organ and cell differentiation in organisms ranging from yeast to humans. most of the work on plant mads-box proteins focused on their roles in floral development whereas less information is available on their function in fruit maturation. we cloned three distinct tomato cdnas using a rt-pcr approach, encoding lemads1, lemads5 and lemads6 factors and whose mrnas mostly accumulate in tomato flowers and fruits. phylogeny analysis indicates that ... | 2010 | 20946942 |
| a kcr1 variant implicated in susceptibility to the long qt syndrome. | the acquired long qt syndrome (alqts) is frequently associated with extrinsic and intrinsic risk factors including therapeutic agents that inadvertently inhibit the kcnh2 k(+) channel that underlies the repolarizing i(kr) current in the heart. previous reports demonstrated that k(+) channel regulator 1 (kcr1) diminishes kcnh2 drug sensitivity and may protect susceptible patients from developing alqts. here, we describe a novel variant of kcr1 (e33d) isolated from a patient with ventricular fibri ... | 2010 | 20950623 |
| sclerostin-erbb-3 interactions: modulation of erbb-3 activity by sclerostin. | to gain insights into the mechanism of action of sclerostin, a protein that regulates bone mass, we performed yeast two-hybrid analyses using human sost (sclerostin) cdna cloned into pgbkt7 dna-binding domain vector as a bait, and a normalized, high-complexity, universal cdna library in a gal4 activating domain vector. we identified an interaction between sclerostin and the carboxyl-terminal portion of the receptor tyrosine-protein kinase erbb-3. to determine the biological relevance of this int ... | 2010 | 20951118 |
| cdk12 is a transcription elongation-associated ctd kinase, the metazoan ortholog of yeast ctk1. | drosophila contains one (dcdk12) and humans contain two (hcdk12 and hcdk13) proteins that are the closest evolutionary relatives of yeast ctk1, the catalytic subunit of the major elongation-phase c-terminal repeat domain (ctd) kinase in saccharomyces cerevisiae, ctdk-i. however, until now, neither cdk12 nor cdk13 has been demonstrated to be a bona fide ctd kinase. using drosophila, we demonstrate that dcdk12 (cg7597) is a transcription-associated ctd kinase, the ortholog of yctk1. fluorescence m ... | 2010 | 20952539 |
| the program for processing newly synthesized histones h3.1 and h4. | the mechanism by which newly synthesized histones are imported into the nucleus and deposited onto replicating chromatin alongside segregating nucleosomal counterparts is poorly understood, yet this program is expected to bear on the putative epigenetic nature of histone post-translational modifications. to define the events by which naive pre-deposition histones are imported into the nucleus, we biochemically purified and characterized the full gamut of histone h3.1-containing complexes from hu ... | 2010 | 20953179 |
| prediction of nucleosome dna formation potential and nucleosome positioning using increment of diversity combined with quadratic discriminant analysis. | in this work, a novel method was developed to distinguish nucleosome dna and linker dna based on increment of diversity combined with quadratic discriminant analysis (idqd), using k-mer frequency of nucleotides in genome. when used to predict dna potential for forming nucleosomes, the model achieved a high accuracy of 94.94%, 77.60%, and 86.81%, respectively, for saccharomyces cerevisiae, homo sapiens, and drosophila melanogaster. the area under the receiver operator characteristics curve of our ... | 2010 | 20953693 |
| camdi, a novel disrupted in schizophrenia 1 (disc1)-binding protein, is required for radial migration. | centrosomes play a crucial role in the directed migration of developing neurons. however, the underlying mechanism is poorly understood. this study has identified a novel disrupted in schizophrenia 1 (disc1)-interacting protein, named camdi after coiled-coil protein associated with myosin ii and disc1, which translocates to the centrosome in a disc1-dependent manner. knockdown of camdi by shrna revealed severely impaired radial migration with disoriented centrosomes. a yeast two-hybrid screen id ... | 2010 | 20956536 |
| backbone (1)h, (15)n, and (13)c resonance assignments and secondary structure of the tollip cue domain. | the toll-interacting protein (tollip) is a negative regulator of the toll-like receptor (tlr)-mediated inflammation response. tollip is a modular protein that contains an nterminal tom1-binding domain (tbd), a central conserved domain 2 (c2), and a c-terminal coupling of ubiquitin to endoplasmic reticulum degradation (cue) domain. here, we report the sequence-specific backbone (1)h, (15)n, and (13)c assignments of the human tollip cue domain. the cue domain was found to be a stable dimer as dete ... | 2010 | 20957454 |
| interaction of testisin with maspin and its impact on invasion and cell death resistance of cervical cancer cells. | previous studies have shown that testisin promotes malignant transformation in cancer cells. to define the mechanism of testisin-induced carcinogenesis, we performed yeast two-hybrid analysis and identified maspin, a tumor suppressor protein, as a testisin-interacting molecule. the direct interaction and cytoplasmic co-localization of testisin with maspin was confirmed by immunoprecipitation and confocal analysis, respectively. in cervical cancer cells, maspin modulated cell death and invasion; ... | 2010 | 20211623 |
| brd7, a subunit of swi/snf complexes, binds directly to brca1 and regulates brca1-dependent transcription. | we carried out a yeast two-hybrid screen using a brca1 bait composed of amino acids 1 to 1142 and identified brd7 as a novel binding partner of brca1. this interaction was confirmed by coimmunoprecipitation of endogenous brca1 and brd7 in t47d and hek-293 cells. brd7 is a bromodomain containing protein, which is a subunit of pbaf-specific swi/snf chromatin remodeling complexes. to determine the functional consequences of the brca1-brd7 interaction, we investigated the role of brd7 in brca1-depen ... | 2010 | 20215511 |
| the conserved mitochondrial twin cx9c protein cmc2 is a cmc1 homologue essential for cytochrome c oxidase biogenesis. | mitochondrial copper metabolism and delivery to cytochrome c oxidase and mitochondrially localized cuzn-superoxide dismutase (sod1) requires a growing number of intermembrane space proteins containing a twin cx(9)c motif. among them, cmc1 was recently identified by our group. here we describe another conserved mitochondrial metallochaperone-like protein, cmc2, a close homologue of cmc1, whose function affects both cytochrome c oxidase and sod1. in the yeast saccharomyces cerevisiae, cmc2 localiz ... | 2010 | 20220131 |
| mitotic catastrophe induced by overexpression of budding yeast rad2p. | mitotic catastrophe provokes endopolyploidy, giant cell formation and, eventually, delayed cell death. mitotic catastrophe is induced by defective cell cycle checkpoints and by some anticancer drugs, ionizing radiation and microtubule-destabilizing agents. rad2 is a yeast homologue of xpg, which is a human endonuclease involved in nucleotide excision repair. here we show that rad2p overexpression alone, in the absence of extrinsic dna damage, causes cell growth arrest and mitotic catastrophe. in ... | 2010 | 20222011 |
| tryptophan scanning analysis of the membrane domain of ctr-copper transporters. | membrane proteins of the ctr family mediate cellular copper uptake in all eukaryotic cells and have been shown to participate in uptake of platinum-based anticancer drugs. despite their importance for life and the clinical treatment of malignancies, directed biochemical studies of ctr proteins have been difficult because high-resolution structural information is missing. building on our recent 7a structure of the human copper transporter hctr1, we present the results of an extensive tryptophan-s ... | 2010 | 20224886 |
| conditional cell-wall mutants of saccharomyces cerevisiae as delivery vehicles for therapeutic agents in vivo to the gi tract. | strains of saccharomyces cerevisiae capable of lysis upon conditional down-regulation of cell-wall biogenesis genes (srb1 and pkc1) have been reported. here, we show that they lyse and release recombinant protein not only under laboratory conditions, but (more importantly) under conditions found in the human stomach and duodenum. these findings provide proof that, in principle, such conditional lysis strains could be used as an integral part of a system for the oral delivery of therapeutic prote ... | 2010 | 20356564 |
| arabidopsis thaliana alg3 mutant synthesizes immature oligosaccharides in the er and accumulates unique n-glycans. | the core oligosaccharide glc(3)man(9)glcnac(2) is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (er). the first step of this assembly pathway on the er luminal side is mediated by alg3 (asparagine-linked glycosylation 3), which is a highly conserved reaction among eukaryotic cells. complementary genetics compared with saccharomyces cerevisiae alg gene families and bioinformatic approaches have en ... | 2010 | 20356820 |
| fatp1 inhibits 11-cis retinol formation via interaction with the visual cycle retinoid isomerase rpe65 and lecithin:retinol acyltransferase. | the isomerization of all-trans retinol (vitamin a) to 11-cis retinol in the retinal pigment epithelium (rpe) is a key step in the visual process for the regeneration of the visual pigment chromophore, 11-cis retinal. lrat and rpe65 are recognized as the minimal isomerase catalytic components. however, regulators of this rate-limiting step are not fully identified and could account for the phenotypic variability associated with inherited retinal degeneration (rd) caused by mutations in the rpe65 ... | 2010 | 20356843 |
| application of stopped-flow kinetics methods to investigate the mechanism of action of a dna repair protein. | transient kinetic analysis is indispensable for understanding the workings of biological macromolecules, since this approach yields mechanistic information including active site concentrations and intrinsic rate constants that govern macromolecular function. in case of enzymes, for example, transient or pre-steady state measurements identify and characterize individual events in the reaction pathway, whereas steady state measurements only yield overall catalytic efficiency and specificity. indiv ... | 2010 | 20357752 |
| mutational analyses of trimethylguanosine synthase (tgs1) and mud2: proteins implicated in pre-mrna splicing. | yeast and human tgs1 are orthologous rna cap (guanine-n2) methyltransferases that convert m(7)g caps into the 2,2,7-trimethylguanosine (tmg) caps characteristic of spliceosomal snrnas. tmg caps are dispensable for vegetative yeast growth, but are essential in the absence of mud2, the putative yeast homolog of human splicing factor u2af. here we exploited the synthetic lethal interactions of tgs1delta and mud2delta mutations to identify essential structural features of the tgs1 and mud2 proteins. ... | 2010 | 20360394 |
| the cargo receptor p24a facilitates calcium sensing receptor maturation and stabilization in the early secretory pathway. | the calcium sensing receptor (casr) is a family 3/c g protein-coupled receptor with slow and partial targeting to the plasma membrane in both native and heterologous cells. we identified cargo receptor family member p24a in yeast two-hybrid screens with the casr carboxyl terminus. interactions were confirmed by immunoprecipitation of either p24a or casr in transiently transfected hek293 cells. only the immaturely glycosylated form of casr interacts with p24a. dissociation likely occurs in the en ... | 2010 | 20361938 |
| the bidirectional cytomegalovirus immediate/early promoter is regulated by hog1 and the stress transcription factors sko1 and hot1 in yeast. | the work presented here intends to address the question of whether the immediate/early promoter of cytomegalovirus (cmv), which is widely used for expressing transgenes in eukaryotic cells, yields a constitutive expression of the transgenes under stress conditions in saccharomyces cerevisiae cells. this information would also be relevant because in the tetracycline-regulated expression (teto) system, which is one of the first choices for studying gene function from yeast to human cells, the cmv ... | 2010 | 20364387 |
| targeted cytosine deaminase-uracil phosphoribosyl transferase suicide gene therapy induces small cell lung cancer-specific cytotoxicity and tumor growth delay. | small cell lung cancer (sclc) is a highly malignant cancer for which there is no curable treatment. novel therapies are therefore in great demand. in the present study we investigated the therapeutic effect of transcriptionally targeted suicide gene therapy for sclc based on the yeast cytosine deaminase (ycd) gene alone or fused with the yeast uracil phosphoribosyl transferase (yuprt) gene followed by administration of 5-fluorocytosine (5-fc) prodrug. experimental design: the ycd gene or the ycd ... | 2010 | 20371678 |
| cc3/tip30 affects dna damage repair. | the pro-apoptotic protein cc3/tip30 has an unusual cellular function as an inhibitor of nucleocytoplasmic transport. this function is likely to be activated under conditions of stress. a number of studies support the notion that cc3 acts as a tumor and metastasis suppressor in various types of cancer. the yeast homolog of cc3 is likely to be involved in responses to dna damage. here we examined the potential role of cc3 in regulation of cellular responses to genotoxic stress. | 2010 | 20374651 |
| yet1p and yet3p, the yeast homologs of bap29 and bap31, interact with the endoplasmic reticulum translocation apparatus and are required for inositol prototrophy. | the mammalian b-cell receptor-associated proteins of 29 and 31 kda (bap29 and bap31) are conserved integral membrane proteins that have reported roles in endoplasmic reticulum (er) quality control, er export of secretory cargo, and programmed cell death. in this study we investigated the yeast homologs of bap29 and bap31, known as yet1p and yet3p, to gain insight on cellular function. we found that yet1p forms a complex with yet3p (yet complex) and that complex assembly was important for subunit ... | 2010 | 20378542 |
| combination of sodium chlorite and calcium propionate reduces enzymatic browning and microbial population of fresh-cut "granny smith" apples. | tissue browning and microbial growth are the main concerns associated with fresh-cut apples. in this study, effects of sodium chlorite (sc) and calcium propionate (cp), individually and combined, on quality and microbial population of apple slices were investigated. "granny smith" apple slices, dipped for 5 min in cp solutions at 0%, 0.5%, 1%, and 2% (w/v) either alone or in combination with 0.05% (w/v) sc, were stored at 3 and 10 degrees c for up to 14 d. color, firmness, and microflora populat ... | 2010 | 20492244 |
| candida species isolated from the gastrointestinal tract of cockatiels (nymphicus hollandicus): in vitro antifungal susceptibility profile and phospholipase activity. | over the past years, the incidence of yeast infections, especially candidiasis, has increased. it is known that birds, including cockatiels, harbor potentially pathogenic yeasts to human beings in their gastrointestinal tract. thus, this work aims at determining the in vitro antifungal susceptibility and phospholipase activity of candida spp. isolated from the gastrointestinal tract and stools of cockatiels. sixty cockatiels were assessed and samples were collected from oral cavity, crop and clo ... | 2010 | 20493645 |
| transferrin fusion technology: a novel approach to prolonging biological half-life of insulinotropic peptides. | fusion proteins made up of glucagon-like peptide 1 (glp-1) and exendin-4 (ex-4) fused to a nonglycosylated form of human transferrin (glp-1-tf or ex-4-tf) were produced and characterized. glp-1-tf activated the glp-1 receptor, was resistant to inactivation by peptidases, and had a half-life of approximately 2 days, compared with 1 to 2 min for native glp-1. glp-1-tf retained the acute, glucose-dependent insulin-secretory properties of native glp-1 in diabetic animals and had a profound effect on ... | 2010 | 20498254 |
| upregulated expression of hitf in crohn's disease and screening of hitf interactant by a yeast two-hybrid system. | to study the expression of human intestinal trefoil factor (hitf) mrna in crohn's disease and to screen the cellular proteins that can interact with the hitf protein by a yeast two-hybrid system in order to explore the mechanism of hitf in protecting intestinal mucosa from injury. | 2010 | 20499178 |
| control of vitellogenin genes expression by sequences derived from transposable elements in rainbow trout. | in most of oviparous animals, vitellogenins (vtg) are the major egg yolk precursors. they are produced in the liver under the control of estrogens. in rainbow trout (oncorhynchus mykiss), the vtg genes cluster contains an unusually large number of almost identical gene copies. in order to identify the regulatory elements in their promoters, we used a combination of reporter plasmids containing genomic sequences including putative estrogen response elements (eres) and we performed transient trans ... | 2010 | 20637911 |
| 14-3-3gamma mediates cdc25a proteolysis to block premature mitotic entry after dna damage. | 14-3-3 proteins control various cellular processes, including cell cycle progression and dna damage checkpoint. at the dna damage checkpoint, some subtypes of 14-3-3 (beta and zeta isoforms in mammalian cells and rad24 in fission yeast) bind to ser345-phosphorylated chk1 and promote its nuclear retention. here, we report that 14-3-3gamma forms a complex with chk1 phosphorylated at ser296, but not at atr sites (ser317 and ser345). ser296 phosphorylation is catalysed by chk1 itself after chk1 phos ... | 2010 | 20639859 |
| secreted ph-regulated antigen 1 of candida albicans blocks activation and conversion of complement c3. | the complement system forms the first defense line of innate immunity and is activated within seconds upon infection by human pathogenic yeast candida albicans. in this study, we identified a new complement evasion strategy used by c. albicans. the fungus secretes a potent complement inhibitor, ph-regulated ag 1 (pra1), which in the direct surrounding of the pathogen binds to fluid-phase c3 and blocks cleavage of c3 to c3a and c3b, as shown by elisa, native gel electrophoresis, and western blott ... | 2010 | 20644161 |
| combining machine learning and homology-based approaches to accurately predict subcellular localization in arabidopsis. | a complete map of the arabidopsis (arabidopsis thaliana) proteome is clearly a major goal for the plant research community in terms of determining the function and regulation of each encoded protein. developing genome-wide prediction tools such as for localizing gene products at the subcellular level will substantially advance arabidopsis gene annotation. to this end, we performed a comprehensive study in arabidopsis and created an integrative support vector machine-based localization predictor ... | 2010 | 20647376 |
| targeting cell division cycle 7 kinase: a new approach for cancer therapy. | the cell division cycle 7 (cdc7) is a serine-threonine kinase, originally discovered in budding yeast, required to initiate dna replication. human cdc7 phosphorylates the minichromosome maintenance protein 2 (mcm2), a component of the dna replicative helicase needed for genome duplication. inhibition of cdc7 in cancer cells impairs progression through s phase, inducing a p53-independent apoptotic cell death, whereas in normal cells, it does not affect cell viability. small molecule compounds abl ... | 2010 | 20647475 |
| [screening and identification of interactive proteins of sh2d4a]. | sh2d4a is a member of sh2 signaling protein family, which is involved in the signal transduction mediated by protein tyrosine kinase-related receptor, cell growth, proliferation, differentiation, and thereby affects the development of human disorders. to determine the role of sh2d4a in the cell signal transduction pathway, sh2d4a interactive proteins were screened using yeast two-hybrid system, and yeast mating and gst pull-down assays were carried out to further confirm the interaction. we succ ... | 2010 | 20650852 |
| the interaction between aid and cib1 is nonessential for antibody gene diversification by gene conversion or class switch recombination. | activation-induced deaminase (aid) initiates somatic hypermutation, gene conversion and class switch recombination by deaminating variable and switch region dna cytidines to uridines. aid is predominantly cytoplasmic and must enter the nuclear compartment to initiate these distinct antibody gene diversification reactions. nuclear aid is relatively short-lived, as it is efficiently exported by a crm1-dependent mechanism and it is susceptible to proteasome-dependent degradation. to help shed light ... | 2010 | 20652029 |
| divergent interactions involving the oxidosqualene cyclase and the steroid-3-ketoreductase in the sterol biosynthetic pathway of mammals and yeasts. | in mammals and yeasts, oxidosqualene cyclase (osc) catalyzes the formation of lanosterol, the first cyclic intermediate in sterol biosynthesis. we used a murine myeloma cell line (ns0), deficient in the 17β-hydroxysteroid dehydrogenase type 7 (hsd17b7), as a model to study the potential interaction of the hsd17b7 with the osc in mammals. hsd17b7 is the orthologue of the yeast steroid-3-ketoreductase (erg27), an enzyme of ergosterol biosynthesis that plays a protective role towards osc. tracer ex ... | 2010 | 20659585 |
| rad3 decorates critical chromosomal domains with gammah2a to protect genome integrity during s-phase in fission yeast. | schizosaccharomyces pombe rad3 checkpoint kinase and its human ortholog atr are essential for maintaining genome integrity in cells treated with genotoxins that damage dna or arrest replication forks. rad3 and atr also function during unperturbed growth, although the events triggering their activation and their critical functions are largely unknown. here, we use chip-on-chip analysis to map genomic loci decorated by phosphorylated histone h2a (gammah2a), a rad3 substrate that establishes a chro ... | 2010 | 20661445 |
| serine-409 phosphorylation and oxidative damage define aggregation of human protein tau in yeast. | unraveling the biochemical and genetic alterations that control the aggregation of protein tau is crucial to understand the etiology of tau-related neurodegenerative disorders. we expressed wild type and six clinical frontotemporal dementia with parkinsonism (ftdp) mutants of human protein tau in wild-type yeast cells and cells lacking mds1 or pho85, the respective orthologues of the tau kinases gsk3β and cdk5. we compared tau phosphorylation with the levels of sarkosyl-insoluble tau (sint), as ... | 2010 | 20662935 |
| shcd interacts with trkb via its ptb and sh2 domains and regulates bdnf-induced mapk activation. | neurotrophins regulate many aspects of neuronal function through activation of the high affinity trk receptors. shc family proteins are implicated in the coupling of rtk to the ras/mitogen-activated protein kinase signaling cascade. here we report that the fourth shc family member, shcd, associates with trkb receptor and regulates bdnf-induced mapk activation. yeast two-hybrid assay and co-ip experiments demonstrate shcd interacts with trkb in a kinase-activity-dependent manner. confocal analysi ... | 2010 | 20663410 |
| rapid identification of candida albicans in blood by combined capillary electrophoresis and fluorescence in situ hybridization. | a ce method based on whole-cell molecular labeling via fluorescence in situ hybridization was developed for the detection of candida albicans in whole blood. removal of potentially interfering red blood cells (rbc) with a simple hypotonic/detergent lysis step enabled us to detect and quantitate contaminating c. albicans cells at concentrations that were orders of magnitude lower than background rbc counts ( approximately 7.0 x 10(9) rbc/ml). in the presence of the lysed blood matrix, yeast cells ... | 2010 | 20665522 |
| characterization of the human hsc20, an unusual dnaj type iii protein, involved in iron-sulfur cluster biogenesis. | the importance of mitochondrial iron-sulfur cluster (isc) biogenesis for human health has been well established, but the roles of some components of this critical pathway still remain uncharacterized in mammals. among them is human heat shock cognate protein 20 (hhsc20), the putative human homolog of the specialized dnaj type co-chaperones, which are crucial for bacterial and fungal isc assembly. here, we show that the human hsc20 protein can complement for its counterpart in yeast, jac1p, and i ... | 2010 | 20668094 |
| identification of binding domains in the herpes simplex virus type 1 small capsid protein pul35 (vp26). | in this study, fragments of the small capsid protein pul35 (vp26) from herpes simplex virus type 1 (hsv-1) were generated to identify binding domains for a number of known ligands. analysis of the binding of dynein light chain subunits, dynlt1 and dynlt3, as well the hsv-1 structural proteins pul19 (vp5) and pul37 was then undertaken using the lexa yeast two-hybrid assay. the n-terminal half of pul35, in particular residues 30-43, was identified as a common region for the binding of dynlt1 and d ... | 2010 | 20668116 |
| identification and biophysical assessment of the molecular recognition mechanisms between the human haemopoietic cell kinase src homology domain 3 and alg-2-interacting protein x. | sfks (src family kinases) are central regulators of many signalling pathways. their functions are tightly regulated through sh (src homology) domain-mediated protein-protein interactions. a yeast two-hybrid screen using sh3 domains as bait identified alix [alg-2 (apoptosis-linked gene 2)-interacting protein x] as a novel hck (haemopoietic cell kinase) sh3 domain interactor. the alix-hck-sh3 interaction was confirmed in vitro by a gst (glutathione transferase) pull-down assay and in intact cells ... | 2010 | 20670214 |