Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| simultaneous quantitative detection of listeria spp. and listeria monocytogenes using a duplex real-time pcr-based assay. | we report a duplex real-time pcr-based assay for the simultaneous quantitative detection of listeria spp. and the food-borne pathogen listeria monocytogenes. the targets of this single tube reaction were the 23s rdna and hly genes of listeria spp. and l. monocytogenes, respectively. our assay was efficient, 100% selective (i.e., it allowed accurate simultaneous identification of 52 l. monocytogenes and 120 listeria spp. strains through the fam-labelled hly and the vic-labelled 23s rdna probes, r ... | 2004 | 15063494 |
| purification and characterization of a recombinant listeriolysin o expressed in escherichia coli and possible diagnostic applications. | the secreted pore-forming toxin listeriolysin o (llo) is an essential virulence factor that allows the food-borne bacterial pathogen listeria monocytogenes to escape from the phagocytic vacuole and reach the host cytosol. this protein belongs to the group of cholesterol-binding sulfhydryl-activated toxins, expressed by a large number of gram-positive bacteria. a protocol for large-scale expression and purification of recombinant llo was previously optimized. by a simple two-step purification met ... | 2004 | 15063610 |
| negative control of listeria monocytogenes virulence genes by a diffusible autorepressor. | virulence genes from the facultative intracellular pathogen listeria monocytogenes are controlled by the transcriptional regulator prfa. although prfa synthesis is activated at 37 degrees c, prfa-dependent expression remains low in rich medium. however, a strong induction of the prfa regulon is observed when l. monocytogenes is cultured in the presence of activated charcoal. here, we show that the 'charcoal effect' results from the adsorption of a diffusible autorepressor substance released by l ... | 2004 | 15066044 |
| the htra (degp) gene of listeria monocytogenes 10403s is essential for optimal growth under stress conditions. | this report describes a mutant of listeria monocytogenes strain 10403s (serotype 1/2a) with a defective response to conditions of high osmolarity, an environment that l. monocytogenes encounters in some ready-to-eat foods. a library of l. monocytogenes clones mutagenized with tn917 was generated and scored for sensitivity to 4% nacl in order to identify genes responsible for growth or survival in elevated-nacl environments. one of the l. monocytogenes tn917 mutants, designated strain osm1, was s ... | 2004 | 15066783 |
| use of pcr-restriction fragment length polymorphism of inla for rapid screening of listeria monocytogenes strains deficient in the ability to invade caco-2 cells. | a pcr-restriction fragment length polymorphism (rflp) method was developed in order to screen a large number of strains for impaired adhesion to epithelial cells due to expression of truncated inla. inla polymorphism was analyzed by pcr-rflp in order to correlate inla pcr-rflp profiles and production of truncated inla. thirty-seven listeria monocytogenes strains isolated from various sources, including five noninvasive and two invasive reference strains, were screened. two endonucleases (alui an ... | 2004 | 15066811 |
| multilocus sequence typing of listeria monocytogenes by use of hypervariable genes reveals clonal and recombination histories of three lineages. | in an attempt to develop a method to discriminate among isolates of listeria monocytogenes, the sequences of all of the annotated genes from the fully sequenced strain l. monocytogenes egd-e (serotype 1/2a) were compared by blastn to a file of the unfinished genomic sequence of l. monocytogenes atcc 19115 (serotype 4b). approximately 7% of the matching genes demonstrated 90% or lower identity between the two strains, and the lowest observed identity was 80%. nine genes (hisj, cbie, trub, ribc, c ... | 2004 | 15066813 |
| pulsed electric field alters molecular chaperone expression and sensitizes listeria monocytogenes to heat. | pulsed electric field (pef)-resistant and pef-sensitive listeria monocytogenes strains were sublethally treated with electric pulses at 15 kv/cm for 29 micro s and held at 25 degrees c for 5 to 30 min prior to protein extraction. the levels of the molecular chaperones groel, groes, and dnaj were determined by immunoblotting. after 10 to 20 min after sublethal pef treatment, a transient decrease in molecular chaperone expression was observed in the pef-sensitive strain (scott a). the levels of gr ... | 2004 | 15066824 |
| genetic markers unique to listeria monocytogenes serotype 4b differentiate epidemic clone ii (hot dog outbreak strains) from other lineages. | a small number of closely related strains of listeria monocytogenes serotype 4b, designated epidemic clone i (eci), have been implicated in numerous outbreaks of food-borne listeriosis described during the past two decades in europe and north america. in 1998 to 1999, a multistate outbreak traced to contaminated hot dogs involved a different strain type of serotype 4b, with genetic fingerprints rarely encountered before. in spite of the profound economic and public health impact of this outbreak ... | 2004 | 15066835 |
| listeriolysin o from listeria monocytogenes is a lymphocyte apoptogenic molecule. | infection of mice with listeria monocytogenes caused marked lymphocyte apoptosis in the white pulp of the spleen on day 2 postinfection. we prove in this study that listeriolysin o (llo), a pore-forming molecule and a major virulence factor of listeria, could directly induce murine lymphocyte apoptosis both in vivo and in vitro at nanomolar and subnanomolar doses. induction of apoptosis by llo was rapid, with caspase activation seen as early as 30 min post-treatment. t cells lost their mitochond ... | 2004 | 15067065 |
| comparative analysis of gene expression among low g+c gram-positive genomes. | we present a comparative analysis of predicted highly expressed (phx) genes in the low g+c gram-positive genomes of bacillus subtilis, bacillus halodurans, listeria monocytogenes, listeria innocua, lactococcus lactis, streptococcus pyogenes, streptococcus pneumoniae, staphylococcus aureus, clostridium acetobutylicum, and clostridium perfringens. most enzymes acting in glycolysis and fermentation pathways are phx in these genomes, but not those involved in the tca cycle and respiration, suggestin ... | 2004 | 15069198 |
| targeting and crossing of the human maternofetal barrier by listeria monocytogenes: role of internalin interaction with trophoblast e-cadherin. | listeria monocytogenes produces severe fetoplacental infections in humans. how it targets and crosses the maternofetal barrier is unknown. we used immunohistochemistry to examine the location of l. monocytogenes in placental and amniotic tissue samples obtained from women with fetoplacental listeriosis. the results raised the possibility that l. monocytogenes crosses the maternofetal barrier through the villous syncytiotrophoblast, with secondary infection occurring via the amniotic epithelium. ... | 2004 | 15073336 |
| bacterial invasion: the paradigms of enteroinvasive pathogens. | invasive bacteria actively induce their own uptake by phagocytosis in normally nonphagocytic cells and then either establish a protected niche within which they survive and replicate, or disseminate from cell to cell by means of an actin-based motility process. the mechanisms underlying bacterial entry, phagosome maturation, and dissemination reveal common strategies as well as unique tactics evolved by individual species to establish infection. | 2004 | 15073367 |
| clinicopathological findings in sheep from sardinia showing neurological signs of disease. | histopathological and bacteriological examinations were performed on 178 brains from sardinian sheep which were showing neurological signs. the sheep represented the total number of sheep with neurological syndromes submitted for diagnostic investigations over a three-year period in sardinia. scrapie was detected in 57 cases, cerebrocortical necrosis in 25, intoxication by a typical mediterranean plant (cistus species) was suspected in 25, coenurosis was detected in 11 cases, listeria monocytoge ... | 2004 | 15074327 |
| risk assessment of listeriosis linked to the consumption of two soft cheeses made from raw milk: camembert of normandy and brie of meaux. | this article reports a quantitative risk assessment of human listeriosis linked to the consumption of soft cheeses made from raw milk. risk assessment was based on data purposefully acquired inclusively over the period 2000-2001 for two french cheeses, namely: camembert of normandy and brie of meaux. estimated listeria monocytogenes concentration in raw milk was on average 0.8 and 0.3 cells/l, respectively, in normandy and brie regions. a monte carlo simulation was used to account for the time-t ... | 2004 | 15078309 |
| modeling growth and reduction of microorganisms in foods as functions of temperature and time. | food safety objectives (fsos) are established in order to minimize the risk of foodborne illnesses to consumers, but these have not yet been incorporated into regulatory policy. an fso states the maximum frequency and/or concentration of a microbiological hazard in a food at the time of consumption that provides an acceptable level of protection to the public and leads to a performance criterion for industry. however, in order to be implemented as a regulation, this criterion has to be achievabl ... | 2004 | 15078311 |
| the diversity of listeria monocytogenes strains from 10 icelandic sheep farms. | the purpose of this study was to examine the diversity of listeria monocytogenes strains from healthy sheep, winter feed and environment of sheep farms in iceland. | 2004 | 15078506 |
| effects of divercin v41 combined to nacl content, phenol (liquid smoke) concentration and ph on listeria monocytogenes scotta growth in bhi broth by an experimental design approach. | to investigate the main effects and interactions of different factors : divercin v41 (0-4 ng ml(-1)), nacl content (0.5-5.5% w v(-1)), phenol (liquid smoke) concentration (0-8 ppm), and ph (5.5-7.5) on listeria monocytogenes scotta growth. | 2004 | 15078508 |
| forces generated during actin-based propulsion: a direct measurement by micromanipulation. | dynamic actin networks generate forces for numerous types of movements such as lamellipodia protrusion or the motion of endocytic vesicles. the actin-based propulsive movement of listeria monocytogenes or of functionalized microspheres have been extensively used as model systems to identify the biochemical components that are necessary for actin-based motility. however, quantitative force measurements are required to elucidate the mechanism of force generation, which is still under debate. to di ... | 2004 | 15079054 |
| a comparison of different chemical sanitizers for inactivating escherichia coli o157:h7 and listeria monocytogenes in solution and on apples, lettuce, strawberries, and cantaloupe. | ozone (3 ppm), chlorine dioxide (3 and 5 ppm), chlorinated trisodium phosphate (100- and 200-ppm chlorine), and peroxyacetic acid (80 ppm) were assessed for reduction of escherichia coli o157:h7 and listeria monocytogenes in an aqueous model system and on inoculated produce. initially, sanitizer solutions were inoculated to contain approximately 10(6) cfu/ml of either pathogen, after which aliquots were removed at 15-s intervals over a period of 5 min and approximately plated to determine log re ... | 2004 | 15083724 |
| evaluation of inoculation method and inoculum drying time for their effects on survival and efficiency of recovery of escherichia coli o157:h7, salmonella, and listeria monocytogenes inoculated on the surface of tomatoes. | a study was undertaken to evaluate methods for applying inoculum and to examine the effect of inoculum drying time on survival and recovery of foodborne pathogens inoculated onto the surface of raw, ripe tomatoes. five-strain mixtures of escherichia coli o157:h7, salmonella, or listeria monocytogenes were applied to tomatoes by dip, spot, or spray inoculation methods. inocula were dried for 1 or 24 h at 22 degrees c before tomatoes were treated with water (control) or chlorine (200 micrograms/ml ... | 2004 | 15083725 |
| influence of variations in methodology on populations of listeria monocytogenes recovered from lettuce treated with sanitizers. | the elimination of listeria monocytogenes inoculated onto a piece of cut iceberg lettuce (3.8 by 3.8 cm) by treatment with chlorinated water (200 micrograms/ml free chlorine) and a 0.5% (wt/vol) solution of fit professional line antibacterial cleaner (fit) was investigated. the efficacy of the two sanitizers was not influenced by the composition of the medium used to culture the l. monocytogenes used in the inocula, the number of strains in the inoculum, or the recovery medium used to enumerate ... | 2004 | 15083726 |
| reduction of microbial pathogens during apple cider production using sodium hypochlorite, copper ion, and sonication. | sodium hypochlorite (100 ppm), copper ion water (1 ppm), and sonication (22 to 44 khz and 44 to 48 khz) were assessed individually and in combination for their ability to reduce populations of escherichia coli o157:h7 and listeria monocytogenes on apples and in apple cider. commercial unpasteurized cider was inoculated to contain approximately 10(6) cfu/ml of either pathogen and then sonicated at 44 to 48 khz, with aliquots removed at intervals of 30 to 60 s for up to 5 min and plated to determi ... | 2004 | 15083729 |
| prevalence and genetic diversity of listeria monocytogenes in the tonsils of pigs. | this study was set up to establish the prevalence of listeria monocytogenes in the tonsils of sows and fattening pigs from five finnish slaughterhouses and to evaluate the genetic similarity of l. monocytogenes strains isolated from the tonsils. a total of 271 pig tonsils (132 tonsils from fattening pigs and 139 from sows) from five different slaughterhouses in various parts of finland were studied from june 1999 to march 2000. overall, 14 and 4% of pig tonsils harbored l. monocytogenes and list ... | 2004 | 15083735 |
| antimicrobial edible films and coatings. | increasing consumer demand for microbiologically safer foods, greater convenience, smaller packages, and longer product shelf life is forcing the industry to develop new food-processing, cooking, handling, and packaging strategies. nonfluid ready-to-eat foods are frequently exposed to postprocess surface contamination, leading to a reduction in shelf life. the food industry has at its disposal a wide range of nonedible polypropylene- and polyethylene-based packaging materials and various biodegr ... | 2004 | 15083740 |
| invasion of the central nervous system by intracellular bacteria. | infection of the central nervous system (cns) is a severe and frequently fatal event during the course of many diseases caused by microbes with predominantly intracellular life cycles. examples of these include the facultative intracellular bacteria listeria monocytogenes, mycobacterium tuberculosis, and brucella and salmonella spp. and obligate intracellular microbes of the rickettsiaceae family and tropheryma whipplei. unfortunately, the mechanisms used by intracellular bacterial pathogens to ... | 2004 | 15084504 |
| microorganisms resistant to free-living amoebae. | free-living amoebae feed on bacteria, fungi, and algae. however, some microorganisms have evolved to become resistant to these protists. these amoeba-resistant microorganisms include established pathogens, such as cryptococcus neoformans, legionella spp., chlamydophila pneumoniae, mycobacterium avium, listeria monocytogenes, pseudomonas aeruginosa, and francisella tularensis, and emerging pathogens, such as bosea spp., simkania negevensis, parachlamydia acanthamoebae, and legionella-like amoebal ... | 2004 | 15084508 |
| virulence and cord blood mononuclear cells cytokine production induced by perinatal listeria monocytogenes strains from different phylogenetic lineages. | some of the phylogenetic lineages of listeria monocytogenes are more likely to cause invasive disease in humans than are strains from other phylogenetic lineages. this suggests that strains belonging to these lineages display different levels of pathogenicity. to investigate this, we carried out a plaque-forming assay with ht-29 cells to evaluate the virulence of six perinatal strains from the three lineages that compose the species. all of the strains were largely over the 3.34 cutoff (between ... | 2004 | 15084811 |
| high affinity, paralog-specific recognition of the mena evh1 domain by a miniature protein. | many protein domains involved in cell signaling contain or interact with proline-rich sequences, and the design of molecules that perturb signaling pathways represents a foremost goal of chemical biology. previously we described a protein design strategy in which the well-folded alpha-helix in avian pancreatic polypeptide (app) presents short alpha-helical recognition epitopes. the miniature proteins designed in this way recognize even shallow protein clefts with high affinity and specificity. h ... | 2004 | 14709031 |
| generation and utilization of polyclonal antibodies to a synthetic c-terminal amino acid fragment of divercin v41, a class iia bacteriocin. | polyclonal antibodies have been generated by immunization of rabbits with a chemically synthesized c-terminal part of divercin v41 (dvnct) conjugated to the carrier protein keyhole limpet hemocyanin (klh). the sensitivity and reactivity of the dvnct-klh-generated antibodies were evaluated by enzyme-linked immunosorbent assay (elisa) using supernatant from cultures of 13 representative lactic acid bacterium strains, and specificity was confirmed by western blot analysis. anti-dvnct-klh antibodies ... | 2004 | 14711648 |
| listeriosis in the pregnant guinea pig: a model of vertical transmission. | feto-placental infections represent a major cause of pregnancy complications, and yet the underlying molecular and cellular mechanisms of vertical transmission are poorly understood. listeria monocytogenes, a facultative intracellular pathogen, is one of a group of pathogens that are known to cause feto-placental infections in humans and other mammals. the purpose of this study was to evaluate possible mechanisms of vertical transmission of l. monocytogenes. humans and guinea pigs have a hemocho ... | 2004 | 14688130 |
| detection of bacteria in suspension by using a superconducting quantum interference device. | we demonstrate a technique for detecting magnetically labeled listeria monocytogenes and for measuring the binding rate between antibody-linked magnetic particles and bacteria. this sensitive assay quantifies specific bacteria in a sample without the need to immobilize them or wash away unbound magnetic particles. in the measurement, we add 50-nm-diameter superparamagnetic magnetite particles, coated with antibodies, to an aqueous sample containing l. monocytogenes. we apply a pulsed magnetic fi ... | 2004 | 14688406 |
| a comparison of the traditional method of counting viable cells and a quick microplate method for monitoring the growth characteristics of listeria monocytogenes. | to determine: (i) the growth parameters (specific growth rate, lag time, asymptotic amount of growth, generation time and time for maximum growth rate) of listeria monocytogenes in different broths by standard cultivation methods and (ii) whether a microplate method in conjunction with a standard nondedicated plate reader could be adapted to routine assay. | 2004 | 14962037 |
| microbiological examination of ready-to-eat cold sliced meats and pâté from catering and retail premises in the uk. | to establish the microbiological quality of cold ready-to-eat sliced meats and pâté from catering and retail premises, and investigate links hypothesized between foodborne campylobacter infection and the consumption of cold sliced meats. | 2004 | 14962130 |
| marinococcus halophilus dsm 20408t encodes two transporters for compatible solutes belonging to the betaine-carnitine-choline transporter family: identification and characterization of ectoine transporter ectm and glycine betaine transporter betm. | in response to osmotic stress, the halophilic, gram-positive bacterium marinococcus halophilus accumulates compatible solutes either by de novo synthesis or by uptake from the medium. to characterize transport systems responsible for the uptake of compatible solutes, a plasmid-encoded gene bank of m. halophilus was transferred into the transport-deficient strain escherichia coli mkh13, and two genes were cloned by functional complementation required for ectoine and glycine betaine transport. the ... | 2004 | 14872322 |
| bacterial pathogen incidences in sludge from swedish sewage treatment plants. | this study surveyed the presence of bacterial pathogens in eight swedish sewage treatment plants (stps), with four different treatment methods, focusing on detection of zoonotic bacteria in raw and treated sludge. salmonella spp., listeria monocytogenes, campylobacter coli and jejuni, escherichia coli o157 and indicator bacteria were investigated. samplings were performed from july 2000 to june 2002, resulting in 64 raw sludge samples and 69 treated sludge samples. the samples from raw sludge (6 ... | 2004 | 15087179 |
| unconventional myosin viia and vezatin, two proteins crucial for listeria entry into epithelial cells. | listeria monocytogenes is a bacterial pathogen with the capacity to invade non-phagocytic cells. this dynamic process involves coordinated membrane remodelling and actin cytoskeleton rearrangements. although some of the molecular factors promoting these events have been identified, the driving force allowing internalization is unknown. one of the receptors for l. monocytogenes on epithelial cells is e-cadherin, a transmembrane protein normally involved in homophilic interactions that allow cell- ... | 2004 | 15090598 |
| nmr solution structure of the precursor for carnobacteriocin b2, an antimicrobial peptide from carnobacterium piscicola. | type iia bacteriocins, which are isolated from lactic acid bacteria that are useful for food preservation, are potent antimicrobial peptides with considerable potential as therapeutic agents for gastrointestinal infections in mammals. they are ribosomally synthesized as precursors with an n-terminal leader, typically 18-24 amino acid residues in length, which is cleaved during export from the producing cell. we have chemically synthesized the full precursor of carnobacteriocin b2, precarnobacter ... | 2004 | 15096213 |
| use of pfge to characterize clonal relationships among belgian clinical isolates of listeria monocytogenes. | the belgian listeria reference centre receives between 30 and 50 human clinical strains of listeria monocytogenes per year. in general, epidemiological data are absent or incomplete, preventing recognition of episodes of listeriosis. however, data on a clonal relationship between strains can indirectly give an idea of the occurrence of episodes. human isolates of l. monocytogenes from 2001 were serotyped, their arsenic-cadmium resistance profiles were determined, and they were pulsotyped with th ... | 2004 | 15096548 |
| [study on national active monitoring for food borne pathogens and antimicrobial resistance in china 2001]. | to survey food borne pathogens and antimicrobial resistance in china. | 2004 | 15098478 |
| changes in peritoneal myeloid populations and their proinflammatory cytokine expression during infection with listeria monocytogenes are altered in the absence of gamma/delta t cells. | evidence that gamma/delta t cells play a broad, immunoregulatory role has been accumulating steadily. we show here that myeloid cells are disregulated after peritoneal infection with listeria monocytogenes in mice lacking gamma/delta t cells. inflammatory populations of neutrophils and monocytes recruited to the site of infection remained longer. intracellular cytokine analysis showed that frequencies of myeloid cells producing interleukin-12 and tumor necrosis factor alpha were higher and remai ... | 2004 | 15107460 |
| modulation of the immune system by listeria monocytogenes-mediated gene transfer into mammalian cells. | in this study, we established a method for listeria monocytogenes(lm)-mediated gene transfer into mammalian cells to manipulate the immune response of the host during infection by pathogens. we used the lm-mediated gene transfer method in an in vivo study to manipulate host immune responses against leishmania major(l. major )-infection. the injection of lm modulated the susceptible host into a resistant state against l. major-infection. a more efficient protective effect was obtained with the in ... | 2004 | 15107544 |
| the antilisterial effect of leuconostoc carnosum 4010 and leucocins 4010 in the presence of sodium chloride and sodium nitrite examined in a structured gelatin system. | to further enhance biopreservation of meat products, the antilisterial effect of the newly described protective culture leuconostoc carnosum 4010 and its bacteriocins, leucocins 4010, was examined in the presence of sodium chloride and sodium nitrite in a solid matrix using a structured gelatin system. interaction between listeria monocytogenes 4140 and leuc. carnosum 4010 or the leucocins 4010-resistant mutant l. monocytogenes 4140p showed that the inhibitory effect of leuc. carnosum 4010 in th ... | 2004 | 15109790 |
| improved protection conferred by vaccination with a recombinant vaccinia virus that incorporates a foreign antigen into the extracellular enveloped virion. | recombinant poxviruses have shown promise as vaccine vectors. we hypothesized that improved cellular immune responses could be developed to a foreign antigen by incorporating it as part of the extracellular enveloped virion (eev). we therefore constructed a recombinant vaccinia virus that replaced the cytoplasmic domain of the b5r protein with a test antigen, hiv-1 gag. mice immunized with the virus expressing gag fused to b5r had significantly better primary cd4 t-cell responses than recombinan ... | 2004 | 15110531 |
| whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen listeria monocytogenes reveal new insights into the core genome components of this species. | the genomes of three strains of listeria monocytogenes that have been associated with food-borne illness in the usa were subjected to whole genome comparative analysis. a total of 51, 97 and 69 strain-specific genes were identified in l.monocytogenes strains f2365 (serotype 4b, cheese isolate), f6854 (serotype 1/2a, frankfurter isolate) and h7858 (serotype 4b, meat isolate), respectively. eighty-three genes were restricted to serotype 1/2a and 51 to serotype 4b strains. these strain- and serotyp ... | 2004 | 15115801 |
| fatal meningitis due to listeria monocytogenes in elderly patients with underlying malignancy. | adult patients with malignancies are considered to be at a high risk for listeria monocytogenes meningitis. the microbiology laboratory's database of the university hospital of ioannina, greece, was searched for cases of l. monocytogenes during the period from january 1990 to december 2002. listerial meningitis occurred in three patients: one with brain tumour, one with chronic lymphocytic leukaemia, and one with non-hodgkin's lymphoma. all the patients were older than 70 and they were actively ... | 2004 | 15117098 |
| fascin-mediated propulsion of listeria monocytogenes independent of frequent nucleation by the arp2/3 complex. | actin-dependent propulsion of listeria monocytogenes is thought to require frequent nucleation of actin polymerization by the arp2/3 complex. we demonstrate that l. monocytogenes motility can be separated into an arp2/3-dependent nucleation phase and an arp2/3-independent elongation phase. elongation-based propulsion requires a unique set of biochemical factors in addition to those required for arp2/3-dependent motility. we isolated fascin from brain extracts as the only soluble factor required ... | 2004 | 15117967 |
| recognition of bacteria in the cytosol of mammalian cells by the ubiquitin system. | recent studies have suggested the existence of innate host surveillance systems for the detection of bacteria in the cytosol of mammalian cells. the molecular details of how bacteria are recognized in the cytosol, however, remain unclear. here we examined the fate of salmonella typhimurium, a gram-negative bacterial pathogen that can infect a variety of hosts, in the cytosol of mammalian cells. these bacteria typically occupy a membrane bound compartment, the salmonella-containing vacuole (scv), ... | 2004 | 15120074 |
| listeriosis. | 2004 | 15121906 | |
| microbial-gut interactions in health and disease. mucosal immune responses. | the host gastrointestinal tract is exposed to countless numbers of foreign antigens and has embedded a unique and complex network of immunological and non-immunological mechanisms, often termed the gastrointestinal 'mucosal barrier', to protect the host from potentially harmful pathogens while at the same time 'tolerating' other resident microbes to allow absorption and utilization of nutrients. of the many important roles of this barrier, it is the distinct responsibility of the mucosal immune ... | 2004 | 15123077 |
| listeria monocytogenes gastroenteritis in sheep. | 2004 | 15768083 | |
| neurolisteriosis causing hydrocephalus, trapped fourth ventricle, hindbrain herniation and syringomyelia. | central nervous system infection by listeria monocytogenes is relatively uncommon, but is known to be highly morbid and fatal. we describe a case of listeria meningoencephalitis, wherein the acute episode was followed by a hitherto unreported conglomeration of severe and progressive neurological sequelae, in the form of supratentorial hydrocephalus, aqueduct block, trapped fourth ventricle, hindbrain herniation and syringomyelia. pertinent literature is reviewed and the pathogenesis of the obser ... | 2004 | 15702836 |
| tumor sensitivity to ifn-gamma is required for successful antigen-specific immunotherapy of a transplantable mouse tumor model for hpv-transformed tumors. | many human tumors lose responsiveness to ifn-gamma, providing a possible mechanism for the tumor to avoid immune recognition and destruction. here we investigate the importance of tumor responsiveness to ifn-gamma in the successful immunotherapy of tc1 tumors that were immortalized with human papillomavirus proteins e6 and e7. | 2004 | 15750832 |
| [listeria monocytogenes detection in different food products and environmental samples from a large chain of supermarkets in the city of bahía blanca (argentina)]. | this work on listeria monocytogenes detection in different foods was carried out between january 2002 and july 2003. ninety cold-served cooked meats, sliced and packaged by different methods and 132 pieces of soft cheeses were studied. these products were analyzed using the presence/absence in 25 g criterion. l. monocytogenes was not found either in foods sliced over the counter or in controlled cheeses, but it was found in 10% of sliced cold-served foods and 5% of cut and cold-served meats vacu ... | 2004 | 15786871 |
| molecular aspects of listeria monocytogenes infection. | listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection. this review is focused on the role of secreted proteins, including listeriolysin o and two distinct phospholipases c, in modulating the signal transduction of infected cells. | 2004 | 15787192 |
| analysis of the peptidoglycan hydrolases of listeria monocytogenes: multiple enzymes with multiple functions. | listeria monocytogenes is a ubiquitous gram-positive, rod-shaped, widespread in nature, facultative intracellular human and animal pathogen that causes infections collectively termed listeriosis. l. monocytogenes egd encodes a total of 133 surface proteins, the abundance of which, as well as the variety of anchoring systems, probably reflects the ability of this bacterium to survive in diverse environments and to interact with many kinds of eukaryotic cells. the group of surface proteins also in ... | 2004 | 15787194 |
| polymerizer-mediated intracellular movement. | bacterial movement inside the cytoplasm is a major virulence factor in that it is necessary for efficient colonization of the infected tissues. molecules from both the host and the pathogen present possible sites of pharmacologic intervention. because locomoting listeria and shigella mimic the activated state of the leading edge of nonmuscle cells, these pathogens are powerful tools for dissecting the molecular machinery of actin-based motility. analysis of the movement linked to cytoskeleton ma ... | 2004 | 15787195 |
| [evaluation of the effect of probiotic cultures added to commercial yogurt over a known population of listeria monocytogenes and escherichia coli o157:h7]. | the effect of probiotic cultures over listeria monocytogenes and escherichia coli o157:h7 during yogurt storage was evaluated. two different yogurt brands, one with additional probiotic cultures (lactobacillus casei and l. acidophilus) were inoculated with known populations (106 ufc/g) of either l. monocytogenes or e. coli o157:h7 in three different times and stored for 32 days at 5 degrees c. every four days the count of lactic bacteria, the added pathogens and ph was evaluated, according to th ... | 2004 | 15807204 |
| cytosolic delivery of viral nucleoprotein by listeriolysin o-liposome induces enhanced specific cytotoxic t lymphocyte response and protective immunity. | cytotoxic t lymphocytes (ctls) are capable of conferring protection against intracellular pathogens and tumor. protective antiviral immunity, mediated by the activation of antigenic epitope-specific ctl, can be achieved by delivering exogenous antigen into the cytosol of antigen-presenting cells. cytosolic introduction of vaccine antigen, however, requires a specialized delivery strategy due to the membrane barrier limiting the access of macromolecules to the cytosol. in this study, we have inve ... | 2004 | 15832496 |
| [analysis of the immune resistance in an experimental murine model fed dietary lipids and infected with listeria monocytogenes]. | several dietary lipids are capable of exerting an immunosupressor effect. this action may have undiserable effects on the host immune resistance to infectious diseases. the purpose of the present study was to determinate the immune status of mice fed dietary lipids and experimentally infected with a virulent strain of listeria monocytogenes. balb/c mice were divided into four groups and were fed with their respective diet: low fat diet (lf, 20%), olive oil diet (oo, 20%), fish oil diet (fo, 20%) ... | 2004 | 15672648 |
| encephalitic listeriosis in ruminants: immunohistochemistry as a diagnostic tool. | a retrospective analysis of 42 ruminants (sheep, goats and cattle) with suspected meningo-encephalitis was performed. the clinical findings and the post-mortem results of the animals have been specified. bacteriological culture, gram's stain and listeria-specific immunohistochemistry were performed in order to confirm the diagnosis of these cases. the results of the different methods were evaluated for the detection of listerial antigens. bacteriological culture was positive in 28.5% of the case ... | 2004 | 15610491 |
| chemical compositions and antibacterial effects of essential oils of turkish oregano (origanum minutiflorum), bay laurel (laurus nobilis), spanish lavender (lavandula stoechas l.), and fennel (foeniculum vulgare) on common foodborne pathogens. | chemical compositions and inhibitory effects of essential oils of turkish oregano (origanum minutiflorum o. schwarz & p. h. davis), bay laurel (laurus nobilis l.), spanish lavender (lavandula stoechas subsp. stoechas l.), and fennel (foeniculum vulgare mill.) on escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium, and staphylococcus aureus were determined. after the essential oils were applied on the foodborne pathogens at doses of 0 (control), 5, 10, 20, 30, 40, 50, and 80 ... | 2004 | 15612826 |
| essential oils of satureja, origanum, and thymus species: chemical composition and antibacterial activities against foodborne pathogens. | the chemical composition of the essential oils obtained from the species restricted to greece and the eastern mediterranean region, satureja spinosa l. and thymus longicaulis l.; species endemic to central and south greece, satureja parnassica ssp. parnassica heldr. and sart ex boiss.; species endemic to the island of crete, origanum dictamnus l.; and species widely distributed in the mediterranean region, satureja thymbra l. and origanum vulgare l. subsp. hirtum, were determined by gas chromato ... | 2004 | 15612827 |
| omega-3 polyunsaturated fatty acids impair in vivo interferon- gamma responsiveness via diminished receptor signaling. | a high intake of omega-3 polyunsaturated fatty acids (n-3 pufas) in mice causes impaired host resistance to listeria monocytogenes. we wished to determine the role of interferon (ifn)-gamma signaling in this increased disease susceptibility. | 2004 | 15633108 |
| temperature and biological soil effects on the survival of selected foodborne pathogens on a mortar surface. | the survival of three foodborne pathogens (listeria monocytogenes, yersinia enterocolitica, and salmonella) attached to mortar surfaces, with or without biological soil (porcine serum) and incubated at either 4 or 10 degrees c in the presence of condensate, was evaluated. soiled and unsoiled coupons were inoculated by immersion into a five-strain cocktail (approximately 10(7) cfu/ml) of each organism type and evaluated. coupons were incubated at 25 degrees c for 2 h to allow attachment of cells, ... | 2004 | 15633668 |
| surface material, temperature, and soil effects on the survival of selected foodborne pathogens in the presence of condensate. | the effects of surface type (stainless steel, acetal resin, and fiberglass reinforced plastic wall paneling [frp]), soil, and temperature on the survival of listeria monocytogenes, salmonella spp., and yersinia enterocolitica, in the presence of condensate were evaluated. surface coupons--half soiled with sterile porcine serum--were exposed to cell suspensions made from individual five-strain cocktails composed of organisms from the same genus (10(7) cfu/ml) in butterfield's phosphate buffer and ... | 2004 | 15633669 |
| mathematical model of listeria monocytogenes cross-contamination in a fish processing plant. | listeriosis is a foodborne disease caused by the bacterium listeria monocytogenes. the food industry and government agencies devote considerable resources to reducing contamination of ready-to-eat foods with l. monocytogenes. because inactivation treatments can effectively eliminate l. monocytogenes present on raw materials, postprocessing cross-contamination from the processing plant environment appears to be responsible for most l. monocytogenes food contamination events. an improved understan ... | 2004 | 15633673 |
| survival of listeria monocytogenes during storage of ready-to-eat meat products processed by drying, fermentation, and/or smoking. | the survival of listeria monocytogenes was evaluated on 15 ready-to-eat meat products made using drying, fermentation, and/or smoking. the products were obtained from six processors and included summer sausage, smoked cured beef, beef jerky, snack stick, and pork rind and crackling products. the water activity of the products ranged from 0.27 (pork rinds and cracklings) to 0.98 (smoked cured beef slices). products were inoculated with a five-strain cocktail of l. monocytogenes, repackaged under ... | 2004 | 15633674 |
| effect of single or sequential hot water and lactic acid decontamination treatments on the survival and growth of listeria monocytogenes and spoilage microflora during aerobic storage of fresh beef at 4, 10, and 25 degrees c. | the survival and growth of listeria monocytogenes and spoilage microflora during storage of fresh beef subjected to different decontamination treatments was studied. fresh beef inoculated with a five-strain mixture of l. monocytogenes (5.18 log cfu/cm2) was left untreated (control) or was immersed (30 s) in hot water (hw; 75 degrees c), 2% lactic acid (la; 55 degrees c), hot water followed by lactic acid (hw-la), or lactic acid followed by hot water (la-hw) and then stored aerobically at 4, 10, ... | 2004 | 15633675 |
| a predictive model for heat inactivation of listeria monocytogenes biofilm on stainless steel. | heat treatment of potential biofilm-forming sites is sometimes used for control of listeria monocytogenes in food processing plants. however, little information is available on the heat treatment required to kill l. monocytogenes present in biofilms. the purpose of this study was to develop a predictive model for the heat inactivation of l. monocytogenes in monoculture biofilms (strains scott a and 3990) and in biofilms with competing bacteria (pseudomonas sp. and pantoea agglomerans) formed on ... | 2004 | 15633676 |
| antibacterial activity of plants used in cooking for aroma and taste. | thirty-three plants used in cooking for aroma and taste were examined for antibacterial activity against pathogens causing foodborne infections. vibrio parahaemolyticus and staphylococcus aureus were sensitive to many kinds of plant extracts, whereas listeria monocytogenes, escherichia coli o157:h7, and salmonella enteritidis populations decreased in only six, one, and three plant extracts, respectively. the polyphenol content in the plants was significantly different between the antibacterial p ... | 2004 | 15633695 |
| carrier status for listeria monocytogenes and other listeria species in free range farm and market healthy indigenous chickens and ducks. | listeria organisms are documented to be zoonotic; one of the sources of infection is the domestic fowl where it could occur as in apparent infection. the carriage of listeria monocytogenes and other listeria in indigenous birds has not been documented in kenya. | 2004 | 15715131 |
| sensitivity of listeria species, recovered from indigenous chickens to antibiotics and disinfectants. | resistance of bacteria to antibiotics and disinfectants has been reported widely in the world. listeria monocytogenes is no exception, although normally it tends to be variably sensitive to many antibiotics and disinfectants. | 2004 | 15715132 |
| application of molecular genetic methods in diagnostics and epidemiology of food-borne bacterial pathogens. | salmonella enterica, campylobacter and yersinia species, shiga toxin-producing escherichia coli (stec), listeria monocytogenes and clostridium perfringens are the bacterial pathogens constituting the greatest burden of food-borne disease in finland. several molecular genetic methods have been applied to diagnose, discriminate and survey these bacteria. pcr, pcr-rflp and pfge are the most widely and successfully used. however, these methods are unable to replace conventional and internationally s ... | 2004 | 15638843 |
| [isolation of listeria monocytogenes from a patient with sealed ruptured thoracoabdominal aortic aneurysm]. | we are reporting a case of sealed rupture of thoracoabdominal aortic aneurysm associated with the isolation of listeria monocytogenes. the patient was a 75-year-old man with previous history of hypertension that had not required medication for the 3 years prior to hospital admission. he was admitted due to chest pain, but he was afebrile. there were no clinical findings indicating infection, although crp was slightly elevated. during his clinical course, a sealed rupture of a thoracoabdominal ao ... | 2004 | 15678977 |
| immunization with a tetramer derivative of an anti-inflammatory pentapeptide produced by entamoeba histolytica protects gerbils (meriones unguiculatus) against experimental amoebic abscess of the liver. | axenically grown entamoeba histolytica produces a pentapeptide (met-gln-cys-asn-ser) with several anti-inflammatory properties, including the inhibition of human monocyte locomotion (monocyte locomotion inhibitory factor (mlif)). a construct displays the same effects as the native material. it remains to be seen if mlif is used, or even produced in vivo by the tissue-invading parasite. if mlif were to be relevant in invasive amoebiasis, immunizing against it could diminish this parasite advantag ... | 2004 | 15679631 |
| a novel mutation within the central listeria monocytogenes regulator prfa that results in constitutive expression of virulence gene products. | the prfa protein of listeria monocytogenes functions as a key regulatory factor for the coordinated expression of many virulence genes during bacterial infection of host cells. prfa activity is controlled by multiple regulatory mechanisms, including an apparent requirement for either the presence of a cofactor or some form of posttranslational modification that regulates the activation of prfa. in this study, we describe the identification and characterization of a novel prfa mutation that resul ... | 2004 | 15342597 |
| rsbt and rsbv contribute to sigmab-dependent survival under environmental, energy, and intracellular stress conditions in listeria monocytogenes. | sigma b (sigma(b)) is a stress-responsive alternative sigma factor that has been identified in various gram-positive bacteria. seven different regulators of sigma b (rsbs) are located in the sigb operons of both bacillus subtilis and listeria monocytogenes. in b. subtilis, these proteins contribute to regulation of sigma(b) activity by conveying environmental and energy stress signals through two well-established branches of a signal transduction pathway. rsbt contributes to regulation of sigma( ... | 2004 | 15345420 |
| molecular fingerprinting of dairy microbial ecosystems by use of temporal temperature and denaturing gradient gel electrophoresis. | numerous microorganisms, including bacteria, yeasts, and molds, constitute the complex ecosystem present in milk and fermented dairy products. our aim was to describe the bacterial ecosystem of various cheeses that differ by production technology and therefore by their bacterial content. for this purpose, we developed a rapid, semisystematic approach based on genetic profiling by temporal temperature gradient electrophoresis (ttge) for bacteria with low-g+c-content genomes and denaturing gradien ... | 2004 | 15345452 |
| inhibition of listeria monocytogenes in fish and meat systems by use of oregano and cranberry phytochemical synergies. | optimized phenolics from oregano and cranberry extracts were evaluated for antimicrobial activity against listeria monocytogenes in laboratory media and in beef and fish. the antimicrobial activity increased when oregano and cranberry extracts were mixed at a ratio of 75% oregano and 25% cranberry (wt/wt) with 0.1 mg of phenolic per disk or ml, and the efficacy was further enhanced by lactic acid. the inhibition by phytochemical and lactic acid synergies was most effective when beef and fish sli ... | 2004 | 15345457 |
| [influence of volatile metabolites of saprophytic soil microflora on the propagation of pathogenic bacteria]. | the influence of volatile metabolites of saprophytic soil microflora on the propagation of listeria monocytogenes and yersinia pseudotuberculosis is shown. different character of interspecific relationships between bacteria, influencing their propagation, can be observed on the metabolic level. volatile compounds produced by microorganisms are capable to act as both intra- and interspecific regulators of microbial communities. in this connection the propagation of pathogenic bacteria inhabiting ... | 2004 | 15346944 |
| cell-surface alterations in class iia bacteriocin-resistant listeria monocytogenes strains. | strains of the food-borne pathogen listeria monocytogenes, showing either intermediate or high-level resistance to class iia bacteriocins, were investigated to determine characteristics that correlated with their sensitivity levels. two intermediate and one highly resistant spontaneous mutant of l. monocytogenes b73, a highly resistant mutant of l. monocytogenes 412, and a highly resistant, defined (mpta) mutant of l. monocytogenes egde were compared with their respective wild-type strains in or ... | 2004 | 15347760 |
| modulation of t cell development and activation by novel members of the schlafen (slfn) gene family harbouring an rna helicase-like motif. | the regulatory networks governing development and differentiation of hematopoietic cells are incompletely understood. members of the schlafen (slfn) protein family have been implicated in the regulation of cell growth and t cell development. we have identified and chromosomally mapped four new members, slfn5, slfn8, slfn9 and slfn10, which belong to a distinct subgroup within this gene family. the characteristic feature of these proteins is the presence of sequence motifs identifying them as dis ... | 2004 | 15351786 |
| antibacterial effect of crude water-soluble arrowroot (puerariae radix) tea extracts on food-borne pathogens in liquid medium. | to evaluate the effect of crude water-soluble arrowroot tea extracts on microbial growth of food-borne pathogens in liquid medium and to confirm the damage to bacterial cells using transmission electronic microscopy (tem). | 2004 | 15355532 |
| fas-fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta t cells. | gammadelta t cells have a direct role in resolving the host immune response to infection by eliminating populations of activated macrophages. macrophage reactivity resides within the vgamma1/vdelta6.3 subset of gammadelta t cells, which have the ability to kill activated macrophages following infection with listeria monocytogenes (lm). however, it is not known how gammadelta t cell macrophage cytocidal activity is regulated, or what effector mechanisms gammadelta t cells use to kill activated ma ... | 2004 | 15356111 |
| the development of functional cd8 t cell memory after listeria monocytogenes infection is not dependent on cd40. | the immunologic requirements for generating long-lived protective cd8 t cell memory remain unclear. memory cd8 populations generated in the absence of cd4 th cells reportedly have functional defects, and at least a subset of cd8 t cells transiently express cd40 after activation, suggesting that direct cd4-cd8 t cell interactions through cd40 may influence the magnitude and functional quality of memory cd8 populations. to ascertain the role of cd40 in such direct t cell interactions, we investiga ... | 2004 | 15356158 |
| time-temperature profiles of chilled ready-to-eat foods in school catering and probabilistic analysis of listeria monocytogenes growth. | the purpose of this study was to evaluate the chill chain in school catering by monitoring time-temperature profiles. chilled ready-to-eat foods have been chosen as subject of this study because of their high risk due to their production, storage and distribution steps, separated in time, followed by consumption without any further thermal treatment. in order to integrate the effects of storage duration and storage temperature, a quantitative criterion, namely "tte" or "time-temperature equivale ... | 2004 | 15358505 |
| molecular epidemiology and disinfectant susceptibility of listeria monocytogenes from meat processing plants and human infections. | we have investigated the molecular epidemiology of listeria monocytogenes from the meat processing industry producing cold cuts and from cases of human listeriosis by discriminative pulsed-field gel electrophoresis (pfge). a subset of the isolates was also investigated for susceptibility to a disinfectant based on quaternary ammonium compounds (qac) frequently used in the meat processing industry. the purpose of this investigation was to obtain knowledge of sources, routes of contamination and g ... | 2004 | 15358509 |
| toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition. | toll-like receptor 2 (tlr2) has been shown to recognize several classes of pathogen-associated molecular patterns including peptidoglycan (pg). however, studies linking pg with tlr2 recognition have relied mainly on the use of commercial staphylococcus aureus pg and have not addressed tlr2 recognition of other pg types. using highly purified pgs from eight bacteria (escherichia coli, pseudomonas aeruginosa, yersinia pseudotuberculosis, helicobacter pylori, bacillus subtilis, listeria monocytogen ... | 2004 | 15359270 |
| evaluation of meat born lactic acid bacteria as protective cultures for the biopreservation of cooked meat products. | in this study, 91 strains, originating from meat products, were subjected to a step-by-step screening and characterisation to search for potential protective cultures to be used in the cooked cured meat industry. strains were first tested on their homofermentative and psychrotrophic character and salt tolerance. secondly, the antibacterial capacities towards listeria monocytogenes, leuconostoc mesenteroides, leuconostoc carnosum and brochotrix thermosphacta were determined in an agar spot test. ... | 2004 | 15364469 |
| growth of listeria monocytogenes as influenced by viscosity and water activity. | the effects of osmotic stress on listeria monocytogenes growth parameters was examined in relation to the viscosity of the growth media. in low-viscosity systems, growth of l. monocytogenes in glucose-supplemented media was comparable to growth in sucrose-supplemented media. the relative lag time (rlt: the lag time divided by the generation time) responses were found to increase in the more restrictive water activity conditions. in high-viscosity systems containing polyvinylpyrrolidone (pvp), gr ... | 2004 | 15364472 |
| listeria-based cancer vaccines that segregate immunogenicity from toxicity. | the facultative intracellular bacterium listeria monocytogenes is being developed as a cancer vaccine platform because of its ability to induce potent innate and adaptive immunity. for successful clinical application, it is essential to develop a listeria platform strain that is safe yet retains the potency of vaccines based on wild-type bacteria. here, we report the development of a recombinant live-attenuated vaccine platform strain that retains the potency of the fully virulent pathogen, comb ... | 2004 | 15365184 |
| a case of listerial meningitis treated with a regimen containing panipenem-betamipron. | although panipenem-betamipron, which is commercially available only in japan, is recommended for treatment of pediatric bacterial meningitis by some experts, only a limited number of clinical studies have been reported. in the present report, we describe a 2-year-old boy with meningitis caused by listeria monocytogenes who was treated with a regimen containing panipenem-betamipron and recovered without any apparent neurological sequelae. on the basis of our experience and previous reports, panip ... | 2004 | 15365867 |
| discrimination of intact and injured listeria monocytogenes by fourier transform infrared spectroscopy and principal component analysis. | fourier transform infrared spectroscopy (ft-ir, 4000-600 cm(-)(1)) was used to discriminate between intact and sonication-injured listeria monocytogenes atcc 19114 and to distinguish this strain from other selected listeria strains (l. innocua atcc 51742, l. innocua atcc 33090, and l. monocytogenes atcc 7644). ft-ir vibrational overtone and combination bands from mid-ir active components of intact and injured bacterial cells produced distinctive "fingerprints" at wavenumbers between 1500 and 800 ... | 2004 | 15366818 |
| expression of housekeeping and immunoproteasome subunit genes is differentially regulated in positively and negatively selecting thymic stroma subsets. | the expression of housekeeping and/or immunoproteasomes in isolated thymic stroma subsets has so far not been analyzed but may have important consequences for self peptide repertoires presented by mhc class i molecules during positive and negative thymic selection. here we determined the expression of housekeeping and immunoproteasome beta subunits and of pa28 in positively and negatively selecting stroma subsets. positively selecting cortical thymic epithelial cells (ctec) expressed only housek ... | 2004 | 15368284 |
| genetic characterization of listeria monocytogenes food isolates and pathogenic potential within serovars 1/2a and 1/2b. | a total of 39 listeria monocytogenes strains isolated from raw milk, smoked meat, chicken carcass and reference strains, belonging to serovars 1/2a, 4a, 1/2b, 3b and 4b, were analysed by rapd and by polymorphisms of the virulent genes inlab and iap. ten isolates, belonging to serovars 1/2a and 1/2b and, collected from raw milk and smoked meat, were further tested for pathogenicity by ip injection into mice. the clustering of the 39 l. monocytogenes strains in 3 groups at 0.45 similarity level, b ... | 2004 | 15368851 |
| sensitivity to ozone, diesel exhaust particles, and standardized ambient particulate matter in rats with a listeria monocytogenes-induced respiratory infection. | ambient particulate matter may increase respiratory allergic skewing of the t-cell-mediated immune response toward a t-helper-2 (th2) response, with the consequence that the th1 response develops less well. successful clearing of a respiratory bacterial infection depends on an adequate th1 immune response; therefore, the subject would not control the infection as well if exposed to particulate matter. to substantiate this hypothesis, we examined the effect of exposure to diesel exhaust particles ... | 2004 | 15371182 |
| glycopeptide derived from hen egg ovomucin has the ability to bind enterohemorrhagic escherichia coli o157:h7. | ovomucin glycopeptide (ogp) was prepared by size exclusion chromatography after pronase digestion of hen egg ovomucin, and the binding of ogp to foodborne pathogens (bacillus cereus,clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, salmonella enteritidis, salmonella typhimurium, and staphylococcus aureus) was investigaed. binding assays with biotinylated bacteria as probes in microtiter plates showed that ogp bound to only e. coli o157:h7 among these foodborne pathogens. ... | 2004 | 15373418 |
| molecular subtyping and tracking of listeria monocytogenes in latin-style fresh-cheese processing plants. | latin-style fresh cheeses, which have been linked to at least 2 human listeriosis outbreaks in the united states, are considered to be high-risk foods for listeria monocytogenes contamination. we evaluated l. monocytogenes contamination patterns in 3 latin-style fresh-cheese processing plants to gain a better understanding of l. monocytogenes contamination sources in the manufacture of these cheeses. over a 6-mo period, 246 environmental samples were collected and analyzed for l. monocytogenes u ... | 2004 | 15375038 |
| prevalence of salmonellae, listeria monocytogenes, and fecal coliforms in bulk tank milk on us dairies. | the objective of this study was to determine the prevalence of salmonella, listeria monocytogenes, and fecal coliforms in bulk tank milk in the united states. as part of the nahms dairy 2002 survey, 861 bulk tank milk samples were collected from farms in 21 states. milk was directly plated on selective agars for direct bacterial enumeration and was enriched in selective broths to increase detection sensitivity. somatic cell counts (scc) and standard plate counts (spc) were also determined. colif ... | 2004 | 15375040 |
| two-dimensional electrophoresis database of listeria monocytogenes egde proteome and proteomic analysis of mid-log and stationary growth phase cells. | listeria monocytogenes is the causative agent of listeriosis, one of the most significant foodborne diseases in industrialized countries. the complete genome of the l. monocytogenes egde strain, belonging to the serogroup 1/2a, has been sequenced and is comprised of 2853 open reading frames. the objective of the current study was to construct a two-dimensional (2-d) database of the proteome of this strain. the soluble protein fractions of the microorganism were recovered either in the mid-log or ... | 2004 | 15378702 |