Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [influence of dairy food rotavirus contamination on morbidity]. | the indirect hemagglutation test (iht) indicated that rotaantigen infection of dairy foods (df) selected at the milk processing shop in the year of high morbidity due to rotavirus infection (rvi) averaged 12.4%. laboratory monitoring of df sold in the year of relative low morbidity could establish that the rate of their rotaantigen contamination averaged 4.8%, as evidenced by iht; polymerase chain reaction was positive in 19.3% of cases. comparison of annual trends in the detection rate of rotaa ... | 2007 | 17343057 |
| [rotavirus infection]. | 2007 | 17350925 | |
| meta-analysis: lactobacillus gg for treating acute diarrhoea in children. | to review evidence for the effectiveness of lactobacillus gg (lgg) in treating acute infectious diarrhoea in children. | 2007 | 17402990 |
| recognition of double-stranded rna by tlr3 induces severe small intestinal injury in mice. | the role of tlrs on intestinal epithelial cells (iecs) is controversial, and the mechanisms by which tlrs influence mucosal homeostasis are obscure. in this study, we report that genomic dsrna from rotavirus, and its synthetic analog polyinosinic-polycytidylic acid (poly(i:c)), induce severe mucosal injury in the small intestine. upon engaging tlr3 on iecs, dsrna triggers iecs to secrete il-15, which functions to increase the percentage of cd3+nk1.1+ intestinal intraepithelial lymphocytes (iels) ... | 2007 | 17372013 |
| expression of nf-kappab in rotavirus-induced damage to the liver and biliary tract in neonatal mice. | biliary atresia, the etiology of which still remains unclear, occurs exclusively in newborns and most are infected with rotavirus. in this study, we aimed to investigate the histopathological patterns of different kinds of rotavirus in the liver and biliary tract of neonatal mice and the expression of nf-kappab in the liver and biliary tract of infected mice. | 2007 | 17374580 |
| [examples for vaccines against diarrheal diseases--rotavirus and traveller's diarrhea]. | diarrheal diseases constitute one of the most important health problems worldwide. children less than 5 years, living in developing countries, are particularly in danger with respect to the incidence and severity of the gastrointestinal disorders. travelers to developing countries are also at risk to develop diarrheal disorders; around 30-50% of them acquire so called "travelers's diarrhea" caused by bacteria, viruses or protozoa. it has been estimated that approximately 30-70% of diarrhea are d ... | 2007 | 17427005 |
| [2006 immunization plan--details of rotavirus vaccination]. | 2007 | 17427011 | |
| human metapneumovirus bronchiolitis in infancy is an important risk factor for asthma at age 5. | human metapneumovirus (hmpv) infection plays an important role in the pediatric respiratory infections. however, little is known about the relationship between hmpv-bronchiolitis and the development of subsequent wheezing. the aim of this study was to evaluate the outcome at third to fifth year after admission for hmpv-bronchiolitis and to compare it with children admitted for respiratory syncytial virus (rsv)-bronchiolitis and with children without lower respiratory disease in the first 2 years ... | 2007 | 17427899 |
| rotavirus infection of infant and young adult nonobese diabetic mice involves extraintestinal spread and delays diabetes onset. | rotaviruses have been implicated as a possible viral trigger for exacerbations in islet autoimmunity, suggesting they might modulate type 1 diabetes development. in this study, the ability of rotavirus strain rrv to infect the pancreas and affect insulitis and diabetes was examined in nonobese diabetic (nod) mice, an experimental model of type 1 diabetes. mice were inoculated either orally or intraperitoneally as infants or young adults. in infant mice inoculated orally, rotavirus antigen was de ... | 2007 | 17428851 |
| [expression of toll-like receptors in mononuclear cells from children with acute rotavirus diarrhea]. | rotavirus is the single most common cause of severe dehydrating diarrhea in young children worldwide, but the pathogenesis and immunity against this disease are not completely understood. a prospective study was conducted to assess gene expression of toll-like receptors (tlr) in children with acute rotavirus diarrhea. | 2007 | 17429531 |
| [risk factors for the occurrence of sporadic campylobacter, salmonella and rotavirus diarrhea in preschool children]. | sporadic cases of diarrhea in preschool children are highly frequent, especially those caused by rotavirus, campylobacter and salmonella. the aim of this study was to identify some of the factors associated with these processes. | 2007 | 17430713 |
| detection of a neonatal human rotavirus strain with vp4 and nsp4 genes of porcine origin. | a human rotavirus strain (nb-150) was detected in stool samples from a neonate hospitalized for mild/moderate community-acquired diarrhoea. this baby lived in the outskirts of belém, brazil, under poor sanitation conditions. the nb-150 strain displayed a typical long electrophoretic pattern with 11 gene segments. it had two vp7 alleles, g1 and g4, and belonged to vp6 subgroup ii. a close relatedness with human rotaviruses was shown for vp7 alleles: g1 (96.9-100 % similarity at the amino acid lev ... | 2007 | 17374895 |
| class b alkaline stabilization to achieve pathogen inactivation. | liming is a cost-effective treatment currently employed in many class b biosolids production plants in the united states. a bench scale model of lime stabilization was designed to evaluate the persistence of viral, bacterial and parasitic pathogens. the survival of fecal coliforms, salmonella, adenovirus type 5, rotavirus wa, bacteriophage ms-2, cryptosporidium parvum oocysts, giardia lamblia cysts, and ascaris lumbricoides ova was evaluated under lime stabilization conditions in a water matrix. ... | 2007 | 17431316 |
| inactivation of adenovirus type 5, rotavirus wa and male specific coliphage (ms2) in biosolids by lime stabilization. | the use of lime to reduce or eliminate pathogen content is a cost-effective treatment currently employed in many class b biosolids production plants in the united states. a bench scale model of lime stabilization was designed to evaluate the survival of adenovirus type 5, rotavirus wa, and the male specific bacteriophage, ms2, in various matrices. each virus was initially evaluated independently in a reverse osmosis treated water matrix limed with an aqueous solution of calcium hydroxide for 24- ... | 2007 | 17431317 |
| how do the rotavirus nsp4 and bacterial enterotoxins lead differently to diarrhea? | rotavirus is the major cause of infantile gastroenteritis and each year causes 611,000 deaths worldwide. the virus infects the mature enterocytes of the villus tip of the small intestine and induces a watery diarrhea. diarrhea can occur with no visible tissue damage and, conversely, the histological lesions can be asymptomatic. rotavirus impairs activities of intestinal disaccharidases and na+-solute symports coupled with water transport. maldigestion of carbohydrates and their accumulation in t ... | 2007 | 17376232 |
| molecular characterization of vp4 and nsp4 genes from rotavirus strains infecting neonates and young children in belém, brazil. | several reports have identified p[6] specificities in humans and in animals in different countries of the world, but few sequence data are available in public databases. in this work we have characterized the vp4 strains bearing p[6] specificity and nsp4 genotypes among diarrheic young children and diarrheic and non-diarrheic neonates from three studies previously conducted in belém, northern region of brazil. as the to vp8* fragment, we observed a close relationship to both human prototypes of ... | 2007 | 17376554 |
| full-length, glycosylated nsp4 is localized to plasma membrane caveolae by a novel raft isolation technique. | rotavirus nsp4, initially characterized as an endoplasmic reticulum intracellular receptor, is a multifunctional viral enterotoxin that induces diarrhea in murine pups. there have been recent reports of the secretion of a cleaved nsp4 fragment (residues 112 to 175) and of the association of nsp4 with lc3-positive autophagosomes, raft membranes, and microtubules. to determine if nsp4 traffics to a specific subset of rafts at the plasma membrane, we isolated caveolae from plasma membrane-enriched ... | 2007 | 17376898 |
| safety, immunogenicity, and protective efficacy of two doses of rix4414 live attenuated human rotavirus vaccine in healthy infants. | to determine the safety, immunogenicity and efficacy of two doses of rotavirus vaccine in healthy brazilian infants. | 2007 | 17380232 |
| enterocolitis due to simultaneous infection with rotavirus and clostridium difficile in adult and pediatric solid organ transplantation. | diarrhea is a well-known complication of immunosuppression but is also frequently caused by pathogens such as clostridium difficile (cd) and rotavirus (rv). three adult and five pediatric solid organ recipients (sors) developed diarrhea with simultaneous identification of cd and rv. rotavirus was identified using an immunochromatografic- or enzyme-linked immunosorbent assay; cd was identified using a rapid immunoassay or enzyme immunoassay. one adult renal, one adult kidney-pancreas, one adult l ... | 2007 | 17440792 |
| diversity of viruses associated with acute gastroenteritis in children hospitalized with diarrhea in ho chi minh city, vietnam. | a molecular epidemiological study on common diarrheal viruses was conducted in ho chi minh city, vietnam between october 2002 and september 2003. fecal samples were collected from 1,010 hospitalized children with acute gastroenteritis. those samples were screened for groups a, b, and c rotavirus, adenovirus, genogroups i and ii norovirus (nov), sapovirus (sav), and human astrovirus (hastv) by rt-multiplex pcr, and the positive specimens were characterized further by elisa, nested pcr, or sequenc ... | 2007 | 17385670 |
| nosocomial rotavirus infection in an australian children's hospital. | 2007 | 17444839 | |
| prevalence of human astrovirus genotypes associated with acute gastroenteritis among children in belém, brazil. | worldwide human astroviruses (hastv) have increasingly been recognized as causative agents of viral gastroenteritis, mainly in infants and young children. the aim of this study was to assess the epidemiology and genotype diversity of hastvs detected in children who participated in a trial in belém, brazil with the rhesus human reassortant rotavirus vaccine tetravalent (rrv-tv). from april/1990 to august/1992, 624 diarrheic stool samples were tested by enzyme immunoassay (eia) for hastv, with a p ... | 2007 | 17385695 |
| detection by pcr of eight groups of enteric pathogens in 4,627 faecal samples: re-examination of the english case-control infectious intestinal disease study (1993-1996). | the english case-control infectious intestinal disease study (1993-1996) failed to detect an enteric pathogen or toxin in 49% of cases of gastroenteritis. in the present study, polymerase chain reaction (pcr) assays were applied to dna and cdna generated from 4,627 faecal samples from cases and controls archived during the original study for the detection of norovirus, rotavirus, sapovirus, campylobacter spp., salmonella spp., enteroaggregative escherichia coli, cryptosporidium spp., and giardia ... | 2007 | 17447091 |
| [viral gastroenteritis. application of a protocol for astrovirus detection in childhood gastroenteritis]. | diagnosis of viral gastroenteritis is an important subject in clinical virology which is mainly determined by the availability of reagents in laboratories, such as in the case of astrovirus. the aim of this study was to estimate the increase in the diagnostic performance achieved after the incorporation of astrovirus search in the diagnosis protocol of acute viral gastroenteritis. we also analyzed the trend of infections in other more commonly searched virus, such as rotavirus and enteric adenov ... | 2007 | 17386241 |
| on a multinational assessment of rotavirus disease in europe. | 2007 | 17387647 | |
| distribution of rotavirus genotypes in europe, 2004-2005: the reveal study. | rotavirus gastroenteritis (rvge) constitutes a significant burden of pediatric disease. knowledge of currently cocirculating rotavirus genotypes is required to help guide immunization strategies. | 2007 | 17387648 |
| [simultaneous outbreaks of rotavirus and respiratory syncytial virus in paris: a 12-year survey]. | the authors had for aim to study the coincidence of rsv and rotavirus epidemic peaks in pediatric patients hospitalized in the paris area. | 2007 | 17459636 |
| genetic characterization of canine rotavirus isolated from a puppy in korea and experimental reproduction of disease. | canine rotavirus was isolated from feces of a korean jindo dog with mild diarrhea, and the isolate was genetically characterized. rotaviral antigen was detected in the feces using a commercial rotavirus antigen detection kit and cytopathic effects were observed in a cell line inoculated with the feces. the virus isolate (gc/ks05) was identified as subtype g3p[3] using reverse transcription polymerase chain reaction (rt-pcr). the strain displayed 98% and 90% identity with the vp7 genes of a canin ... | 2007 | 17459836 |
| rotavirus and celiac disease: clues to the pathogenesis and perspectives on prevention. | 2007 | 17460483 | |
| clinical consequences of rotavirus acute gastroenteritis in europe, 2004-2005: the reveal study. | the availability of comprehensive, up-to-date epidemiologic data would improve the understanding of the disease burden and clinical consequences of rotavirus gastroenteritis (rvge) in europe. | 2007 | 17387649 |
| multicenter prospective study of the burden of rotavirus acute gastroenteritis in europe, 2004-2005: the reveal study. | rotavirus is recognized as a significant cause of pediatric gastroenteritis worldwide. comprehensive data on the burden of rotavirus disease in europe were lacking. | 2007 | 17387650 |
| rotavirus mortality confirmed by etiologic identification in venezuelan children with diarrhea. | hospital-based studies to determine the etiology of deaths from diarrhea are scarce. in this study, we specifically analyzed deaths due to rotavirus to assess the rotavirus impact on diarrhea mortality. | 2007 | 17468648 |
| rotavirus vaccines in developed countries. | rotavirus is the most common cause of diarrhoea and dehydration in early childhood. the recent licensure in many nations of vaccines against rotavirus offers promise to significantly reduce this toll. the present review describes recent developments regarding rotavirus vaccines and the challenges they face. | 2007 | 17471034 |
| [merck's oral rotavirus vaccine rotateq suspected to cause invagination in infants]. | 2007 | 17393857 | |
| evaluating rotavirus vaccination in england and wales. part ii. the potential cost-effectiveness of vaccination. | rotavirus is the leading cause of acute gastroenteritis in children. two rotavirus vaccines (rotateq and rotarix) have recently completed clinical trials. we investigated whether routine infant immunisation with either vaccine can be cost effective. | 2007 | 17400341 |
| health risks in wastewater irrigation: comparing estimates from quantitative microbial risk analyses and epidemiological studies. | the combination of standard quantitative microbial risk analysis (qmra) techniques and 10,000-trial monte carlo risk simulations was used to estimate the human health risks associated with the use of wastewater for unrestricted and restricted crop irrigation. a risk of rotavirus infection of 10(-2) per person per year (pppy) was used as the reference level of acceptable risk. using the model scenario of involuntary soil ingestion for restricted irrigation, the risk of rotavirus infection is appr ... | 2007 | 17402278 |
| infection is the major component of the disease burden in aboriginal and non-aboriginal australian children: a population-based study. | infection accounts for the majority of pediatric mortality and morbidity in developing countries, but there are limited data on the infectious diseases burden in children from developed countries. we investigated reasons for hospitalization before age 2 years in a birth cohort of western australian aboriginal and non-aboriginal children. | 2007 | 17484216 |
| concomitant use of an oral live pentavalent human-bovine reassortant rotavirus vaccine with licensed parenteral pediatric vaccines in the united states. | a live pentavalent rotavirus vaccine (prv) containing 5 human-bovine (wc3) reassortants expressing human serotypes g1, g2, g3, g4 and p1a[8] was evaluated in a blinded, placebo-controlled study. possible interactions between prv and concomitantly administered licensed pediatric vaccines were investigated in a united states-based nested substudy (concomitant use study) of the rotavirus efficacy and safety trial. | 2007 | 17484218 |
| hospital surveillance of rotavirus infection and nosocomial transmission of rotavirus disease among children in guinea-bissau. | vaccination against rotavirus is protective against severe disease. surveillance of rotavirus infection in developing countries might direct vaccination policy more efficiently. | 2007 | 17484220 |
| detection of viral agents in fecal specimens of monkeys with diarrhea. | diarrheal disease is a major cause of morbidity and mortality in humans and animals, including non human primates. while the diagnostics for gastrointestinal bacterial and parasitic pathogens and their etiological role in disease are well established, little is known about the epidemiology, prevalence and role of viral agents in diarrheal illness among monkeys. | 2007 | 17493140 |
| transfer of maternal cytokines to suckling piglets: in vivo and in vitro models with implications for immunomodulation of neonatal immunity. | maternal cytokines may play instructive roles in development of the neonatal immune system. however, cytokines in colostrum and milk and their transfer from mothers to neonates have not been well documented, except for tgf-beta. swine provide a unique model to study lactogenic cytokines because the sow's impermeable placenta prohibits transplacental passage. we investigated il-6 and tnf-alpha (pro-inflammatory), ifn-gamma and il-12 (th1), il-10 and il-4 (th2) and tgf-beta1 (th3) concentrations i ... | 2007 | 17403542 |
| molecular characterization of vp4 and vp7 genes of nontypeable strains identifies a novel p[28] genotype in porcine rotaviruses. | this article has been retracted. | 2007 | 17409206 |
| intussusception alert. | 2007 | 17413328 | |
| epidemiology and burden of rotavirus-associated hospitalizations in ferrara, italy. | objective of this study was to provide data on hospitalizations for rotavirus gastroenteritis (rvge) in ferrara, italy. the study was conducted analyzing the hospital discharge forms of all children admitted to the pediatric department of the university of ferrara, arcispedale sant'anna, from january 2001 through december 2005. the database was searched for all gastrointestinal diseases and in particular rvge. during the period under study 3277 children, of which 2038 <60 months of age, were hos ... | 2007 | 17506231 |
| an nsp4-dependant mechanism by which rotavirus impairs lactase enzymatic activity in brush border of human enterocyte-like caco-2 cells. | lactase-phlorizin hydrolase (lph, ec 3.2.1.23-62) is a brush border membrane (bbm)-associated enzyme in intestinal cells that hydrolyse lactose, the most important sugar in milk. impairing in lactase activity during rotavirus infection has been described in diseased infants but the mechanism by which the functional lesion occurs remains unknown. we undertook a study to elucidate whether rotavirus impairs the lactase enzymatic activity in bbm of human enterocyte cells. in this study we use cultur ... | 2007 | 17506819 |
| rotaviruses: diversity and zoonotic potential--a brief review. | rotaviruses, a genus within the family reoviridae, are among the most important etiological agents of severe diarrhoeal illness in humans and animals worldwide. their genome, consisting of 11 segments of double-stranded rna, is characterized by genetic variability including (i) point mutations, (ii) genomic reassortment, and (iii) genome rearrangements, thus leading to the considerable diversity of rotaviruses. animal rotaviruses are regarded as a potential reservoir for genetic exchange with hu ... | 2007 | 17416132 |
| molecular analysis of the nsp4 and vp6 genes of rotavirus strains recovered from hospitalized children in rio de janeiro, brazil. | group a rotaviruses are the main cause of acute gastroenteritis in children throughout the world. the two outer capsid proteins, vp4 and vp7, define the p and g genotypes, respectively. rotaviruses with p[8]g1, p[4]g2, p[8]g3 and p[8]g4 genotypes are predominant in infecting humans and the g9 genotype is emerging in most continents as the fifth most common g type worldwide. the inner capsid protein vp6 is responsible for subgroup (sg) specificities, allowing classification of rotaviruses into sg ... | 2007 | 17510274 |
| etiologic agents of diarrhea in solid organ recipients. | after transplantation, diarrhea may be caused by infectious agents, drug-specific effects, metabolic conditions, or mechanical complications of surgery. determining the cause helps to determine whether to initiate antimicrobial therapy and the duration of treatment. in this study we aimed to determine the causes of diarrhea in kidney or liver recipients. fifty-two diarrhea episodes among 43 solid organ recipients were evaluated. the cause of diarrhea was detected in 43 patients (82.6%). infectio ... | 2007 | 17511817 |
| prevalence of group c rotaviruses in weaning and post-weaning pigs with enteritis. | diarrheic fecal specimens collected from porcine herds were screened for the presence of group c rotaviruses using a reverse transcription-polymerase chain reaction (rt-pcr) assay. a total of 188 samples were tested and 54 were positive. when compiled these data with diagnostic results on group a rotaviruses and enteric caliciviruses we found that all but 5 group c rotavirus positive samples contained at least one additional virus. a subset of samples were subjected to nucleotide sequencing. the ... | 2007 | 17416471 |
| detection of the rotavirus a genome from the cerebrospinal fluid of a gastroenteritis patient: a case report. | 2007 | 17515656 | |
| [clinical and microbiological features of salmonella gastroenteritis in children]. | the aim of our study was to evaluate the role of salmonella spp in children hospitalised for acute gastroenteritis, and to study clinical and microbiological features of paediatric salmonellosis in our geographical area. in all, 540 patients admitted from march to september 2003 with symptoms of acute enteritis to the infectious diseases department of the "g. di cristina" hospital in palermo were enrolled. stool samples were collected within 48 hours of admission and tested for intestinal pathog ... | 2007 | 17515672 |
| molecular characterization of a new porcine rotavirus p genotype found in an asymptomatic pig in slovenia. | rotaviral rna was detected in the stool sample of an asymptomatic fattening pig at a slovenian pig farm. to characterize the rotavirus, rt-pcr was used, employing primers specific for the vp7, vp4 and nsp4 genes. specific products were purified and the sequencing reaction was performed for the molecular analysis of amplified genes. nucleotide and amino acid sequences of the vp7 gene were found highly identical (85.3-88.1% and 90.7-91.6%) to g1 genotype strains. phylogenetic and molecular analyse ... | 2007 | 17078990 |
| fluoro-ketopyranosyl nucleosides: synthesis and biological evaluation of 3-fluoro-2-keto-beta-d-glucopyranosyl derivatives of n4-benzoyl cytosine. | 1,2:5,6-di-o-isopropylidene-alpha-d-glucofuranose on mild oxidation, reduction, fluorination, and deisopropylidenation followed by acetylation gave peracetylated 3-deoxy-3-fluoro-d-glucopyranose. this was coupled with silylated n(4)-benzoyl cytosine. the nucleoside was deacetylated and after several subsequent protection and deprotection steps afforded the desired 3-fluoro-2-keto-beta-d-glucopyranosyl derivatives. these novel synthesized compounds were evaluated for antiviral and cytotoxic activ ... | 2007 | 17079149 |
| [nosocomial rotavirus infections in a pediatric unit: surveillance during four successive winters]. | the incidence of rotavirus and rsv outbreaks during winter seasons leads to overcrowding of pediatric units in the paris area, and increases the risk of viral nosocomial infections in hospitalized young infants. | 2007 | 17150322 |
| rotavirus g9 severity data revisited. | 2007 | 17143842 | |
| diversity in indian equine rotaviruses: identification of genotype g10,p6[1] and g1 strains and a new vp7 genotype (g16) strain in specimens from diarrheic foals in india. | rotaviruses causing severe diarrhea in foals in two organized farms in northern india, during the period from 2003 to 2005, were characterized by electropherotyping, serotyping, and sequence analysis of the genes encoding the outer capsid proteins. of 137 specimens, 47 (34.31%) were positive for rotavirus and exhibited at least five different electropherotypes (e), e1 to e5. strains belonging to different electropherotypes exhibited either a different serotype/genotype specificity or a lack of r ... | 2007 | 17135436 |
| geographic information systems and genotyping in identification of rotavirus g12 infections in residents of an urban slum with subsequent detection in hospitalized children: emergence of g12 genotype in south india. | rotavirus infections by g12 strains in several countries have recently been described. in this study, we report the emergence of g12 strains in south india. fourteen cases of g12 infection were identified between june and september 2005. g12 was seen in combination with p[6], p[8], or nontypeable p type. nine cases, including five symptomatic infections and four asymptomatic infections, were identified as part of routine surveillance for rotavirus infections in a birth cohort in the community be ... | 2007 | 17135437 |
| genome heterogeneity of sa11 rotavirus due to reassortment with "o" agent. | derivatives of the rotavirus sa11-h96 strain, isolated in 1958 from an overtly healthy vervet monkey, have been used extensively to probe the viral life cycle. to gain insight into the phenotypic and genotypic differences among sa11 isolates, we sequenced the segmented double-stranded rna genomes of sa11-h96 (p5b[2]:g3), two sa11-4f-like viruses (p6[1]:g3), two sa11-4f-like viruses with gene 5 rearrangements, and relevant segments of sa11 temperature-sensitive mutants and the "o" (offal) agent ( ... | 2007 | 17059839 |
| rotavirus: to the gut and beyond! | rotavirus causes severe gastroenteritis in children. a principle of rotavirus pathogenesis has been that the infection remains localized to epithelial cells in the small intestine. this dogma was challenged by recent findings of rotavirus in the serum of experimentally infected animals and children with diarrhea. repeated associations of rotavirus infections with a wide range of nongastroenteric clinical manifestations in humans were considered spurious because of lack of proof that rotavirus es ... | 2007 | 17133083 |
| rotavirus vp7, vp4 and vp6 genotypes co-circulating in tehran, iran, between 2003 and 2004. | rotaviruses were detected by enzyme-linked immunosorbent assay (elisa) in 92 out of 374 faecal samples collected between november 2003 and october 2004 at the markaz tebbi koudakan hospital, tehran, iran, from children aged 6 months to 5 years. analysis of clinical and disease severity data showed a significant association between rotavirus infection and diarrhoea, vomiting and severe dehydration. ninety-two samples (64 rotavirus elisa-positive and 28 elisa-negative samples) were sent to the ent ... | 2007 | 17109772 |
| [evaluation of seven immunochromatographic assays for the rapid detection of human rotaviruses in fecal specimens]. | seven commercially available immunochromatographic assays were tested for the rapid detection of group a rotaviruses in fecal samples compared to a enzyme immunoassay (argene). detection of rotaviruses in 80 elisa positive frozen stool samples showed rates superior to 90% for three reagents (rota strip (cypress diagnostics), 98.8%; rotascreen (microgen), 95.0%; vikia rota/adeno (biomérieux), 92.5%); from 82.5% to 88.8% for three others (diarlex with centrifugation (orion diagnostica), 88.8%; com ... | 2007 | 17049423 |
| effectiveness of childhood vaccination against rotavirus in sub-saharan africa: the case of nigeria. | rotavirus diarrhoea is associated with high childhood mortality in developing countries. a new vaccine was recently licensed in mexico. the objective of this study was to assess the effectiveness of routine childhood vaccination by this new vaccine in a developing country. we constructed a decision tree to compare two alternatives: "no vaccination programme" and "vaccination programme". the estimates used for disease incidence, vaccine efficacy and coverage rates were derived from published data ... | 2007 | 17055130 |
| identification of group a porcine rotavirus strains bearing a novel vp4 (p) genotype in italian swine herds. | the vp4 gene of a g5 italian porcine rotavirus strain, 344/04-1, was nontypeable by pcr genotyping. the amino acid sequence of the full-length vp4 protein had low identity (<or=76.6%) with the homologous sequences of representative strains of the remaining p genotypes, providing evidence for a novel p genotype. | 2007 | 17122003 |
| genotypes and subtypes of cryptosporidium spp. in neonatal calves in northern ireland. | cryptosporidium spp. in diarrheic calves less than 30 days old from farms across northern ireland were examined over a year period by microscopic, genotyping, and subtyping techniques to characterize the transmission dynamics. cryptosporidium oocysts were detected in 291 of 779 (37.4%) animals. the prevalence rates of rotavirus, coronavirus, and escherichia coli k99+ were lower as seen in 242 of 806 (30.0%), 46/806 (5.7%), and 16/421 (3.8%) of animals, respectively. of the 224 cryptosporidium-po ... | 2007 | 17031699 |
| genetic distribution of group a human rotavirus types isolated in gyunggi province of korea, 1999-2002. | human rotavirus genotypes g1-g4 and g9 are the major etiological agents of infantile gastroenteritis. g1 was the most prevalent in korea during the 10-year period prior to 1997. however, between 1998 and 1999, g4 was the predominant type in korea, as it was in other asian countries. | 2007 | 17118702 |
| viral infections as potential triggers of type 1 diabetes. | during the last decades, the incidence of type 1 diabetes (t1d) has increased significantly, reaching percentages of 3% annually worldwide. this increase suggests that besides genetical factors environmental perturbations (including viral infections) are also involved in the pathogenesis of t1d. t1d has been associated with viral infections including enteroviruses, rubella, mumps, rotavirus, parvovirus and cytomegalovirus (cmv). although correlations between clinical presentation with t1d and th ... | 2007 | 17103489 |
| rotavirus infections in paediatric oncology patients: a matched-pairs analysis. | to conduct a systematic investigation of the clinical relevance of rotavirus infection in the setting of paediatric cancer patients receiving intensive chemotherapy. | 2007 | 17190767 |
| impact of rotavirus infections on outpatient clinic visits in chile. | incorporation of new rotavirus vaccines into national programs of developing countries will rely on well-designed cost-effective analysis based on accurate assessment of disease burden. for chile, rotavirus disease burden is determined mostly by outpatient clinic and emergency room visits and by hospitalizations. we previously estimated a yearly incidence of 8000 and 53,000 hospitalizations and emergency room visits respectively for children <or=3 years of age. | 2007 | 17195704 |
| genetic characterization of group a rotavirus strains circulating among children with acute gastroenteritis in japan in 2004-2005. | a total of 752 fecal specimens collected from july 2004 to june 2005 from children with acute gastroenteritis in four localities in japan (maizuru, tokyo, sapporo, and osaka) were screened for group a rotavirus by rt-pcr. it was found that 82 (10.9%) specimens were positive for group a rotavirus. the g-(vp7 genotypes) and p-(vp4 genotypes) types were further investigated. the p-types of 18 rotavirus strains, which could not be typed by rt-pcr, were determined by sequencing analysis. of these, 94 ... | 2007 | 17110174 |
| interaction of rotavirus polymerase vp1 with nonstructural protein nsp5 is stronger than that with nsp2. | rotavirus morphogenesis starts in intracellular inclusion bodies called viroplasms. rna replication and packaging are mediated by several viral proteins, of which vp1, the rna-dependent rna polymerase, and vp2, the core scaffolding protein, were shown to be sufficient to provide replicase activity in vitro. in vivo, however, viral replication complexes also contain the nonstructural proteins nsp2 and nsp5, which were shown to be essential for replication, to interact with each other, and to form ... | 2007 | 17182692 |
| heterogeneity of raft-type membrane microdomains associated with vp4, the rotavirus spike protein, in caco-2 and ma 104 cells. | previous studies have shown that rotavirus virions, a major cause of infantile diarrhea, assemble within small intestinal enterocytes and are released at the apical pole without significant cell lysis. in contrast, for the poorly differentiated kidney epithelial ma 104 cells, which have been used extensively to study rotavirus assembly, it has been shown that rotavirus is released by cell lysis. the subsequent discovery that rotavirus particles associate with raft-type membrane microdomains (rtm ... | 2007 | 17135322 |
| intestinal ribosomal p70(s6k) signaling is increased in piglet rotavirus enteritis. | recent identification of the mammalian target of rapamycin (mtor) pathway as an amino acid-sensing mechanism that regulates protein synthesis led us to investigate its role in rotavirus diarrhea. we hypothesized that malnutrition would reduce the jejunal protein synthetic rate and mtor signaling via its target, ribosomal p70 s6 kinase (p70(s6k)). newborn piglets were artificially fed from birth and infected with porcine rotavirus on day 5 of life. study groups included infected (fully fed and 50 ... | 2007 | 17138969 |
| antiviral effects on bacteriophages and rotavirus by cranberry juice. | studies were undertaken to investigate the antiviral effects of comestible juices, especially cranberry juice, on non-related viral species. after exposure of bacteriophage t2 to a commercially available cranberry (vaccinium macrocarpon) juice cocktail (cj), virus infectivity titer was no longer detectible. after a 60-min exposure to orange (oj) and grapefruit juices (gj), phage infectivity was reduced to 25-35% of control, respectively. similar data were observed for the bacteriophage t4. cj in ... | 2007 | 17140784 |
| studies on human rotavirus (hrv) vaccination. | 2007 | 17204972 | |
| effect of rotavirus strain on the murine model of biliary atresia. | biliary atresia is a devastating disorder of the newborn in which afflicted infants develop inflammation and fibrosis of the extrahepatic biliary tract, resulting in cirrhosis and end-stage liver disease. infection with a virus is thought to be a contributing factor in the etiology of biliary atresia. in the murine model of biliary atresia, perinatal exposure to rhesus rotavirus (rrv) results in biliary epithelial cell infection causing bile duct obstruction. the purpose of this study was to det ... | 2007 | 17121809 |
| evolutionary history and global spread of the emerging g12 human rotaviruses. | g12 rotaviruses were first detected in diarrheic children in the philippines in 1987, but no further cases were reported until 1998. however, g12 rotaviruses have been detected all over the world in recent years. here, we report the worldwide variations of g12 rotaviruses to investigate the evolutionary mechanisms by which they managed to spread globally in a short period of time. we sequenced the complete genomes (11 segments) of nine g12 rotaviruses isolated in bangladesh, belgium, thailand, a ... | 2007 | 17166908 |
| anticipating rotavirus vaccines in brazil: detection and molecular characterization of emerging rotavirus serotypes g8 and g9 among children with diarrhoea in recife, brazil. | in 2006, brazil will initiate universal immunization of its 4-million infants with a live attenuated serotype g1p[8] human rotavirus vaccine. in anticipation of the national immunization program, this study was undertaken to characterize rotavirus strains circulating among children in recife, one of the largest cities in the northeast region of brazil. group a rotaviruses were detected in 102 (35%) of 290 faecal specimens collected from children under 5 years of age who presented with acute diar ... | 2007 | 17245712 |
| molecular characterization of a new variant of rotavirus p[8]g9 predominant in a sentinel-based survey in central italy. | rotavirus p[8]g9 was recognized as the most widespread genotype during a sentinel-based survey in italy; phylogenetic analysis of the vp7 and vp4 genes showed that italian isolates constituted a closely related genetic cluster distinct from the other g9 strains recently isolated in other european countries, america, and asia. | 2007 | 17166955 |
| biotin-psolaren labeled cdna amplicons for genotyping rotavirus strains by dot hybridization assay. | a simple method to produce biotinylated probes by utilizing an amine-psoralen to conjugate an ester-biotin to nucleic acid molecules is described. it is simple, rapid, and well suited to label cdna probes with any pcr-generated amplicon for hybridization assay. its application to identify or to confirm the g and p genotypes of rotavirus-derived amplicons is described; however, it may be used to label amplicons of any origin. as an alternative or as a complementary test to either pcr-typing assay ... | 2007 | 17169439 |
| an outbreak of adenovirus serotype 41 infection in infants and children with acute gastroenteritis in maizuru city, japan. | a total of 337 fecal specimens were collected from infants and children with acute gastroenteritis in maizuru city, japan from july 2004 to june 2005 and tested for the presence of rotavirus, norovirus, sapovirus, astrovirus, and adenovirus by rt-multiplex pcr. among diarrheal viruses detected, norovirus was the most prevalent (13.6%, 46 of 337), followed by adenovirus (8%, 27 of 337), group a rotavirus (5%, 17 of 337), astrovirus (1.8%, 6 of 337), and sapovirus (1.8%, 6 of 337), respectively. a ... | 2007 | 17157081 |
| rotavirus disease in uzbekistan: cost-effectiveness of a new vaccine. | we evaluated the cost-effectiveness of rotavirus vaccination in uzbekistan from the healthcare system and societal perspectives. disease burden was estimated using national statistics on hospitalizations and deaths, and international estimates of under-five mortality. without vaccination, the risk for rotavirus hospitalization by age 5 is 10 per 1000 children. rotavirus hospitalizations cost us$ 406,000 annually, of which us$ 360,000 (89%) is for medical expenses and us$ 46,000 (11%) is for non- ... | 2007 | 16930784 |
| detection of genogroup i and ii human picobirnaviruses showing small genomic rna profile causing acute watery diarrhoea among children in kolkata, india. | picobirnaviruses (pbvs) with bisegmented small rna genome profile (1.75 and 1.55kbp for segment 1 and 2, respectively) were detected from 1999 to 2003 in faecal specimens of acute watery diarrhoea cases, largely children (n=20) and an adult in kolkata, india. varying degrees of dehydration necessitated their visit to hospital for further treatment and management of acute watery diarrhoea. pbv was associated with rotavirus (n=3) or astrovirus (n=3) and with both in one case. no co-infection with ... | 2007 | 17049316 |
| the epidemiology and burden of rotavirus in china: a review of the literature from 1983 to 2005. | we reviewed studies of rotavirus in medline and the chinese literature to get a preliminary estimate of the burden of rotavirus gastroenteritis in china and the epidemiology of the disease. studies were selected if they were conducted for a period 1 year or more, had more than 100 patients enrolled, and used an accepted diagnostic test. overall, in 27 reports of children hospitalized for diarrhea in urban areas and 3 in rural areas, 44 and 33%, respectively, had rotavirus identified as the etiol ... | 2007 | 16956700 |
| std nmr spectroscopy and molecular modeling investigation of the binding of n-acetylneuraminic acid derivatives to rhesus rotavirus vp8* core. | the vp8* subunit of rotavirus spike protein vp4 contains a sialic acid (sia)-binding domain important for host cell attachment and infection. in this study, the binding epitope of the n-acetylneuraminic acid (neu5ac) derivatives has been characterized by saturation transfer difference (std) nuclear magnetic resonance (nmr) spectroscopy. from this std nmr data, it is proposed that the vp8* core recognizes an identical binding epitope in both methyl alpha-d-n-acetylneuraminide (neu5acalpha2me) and ... | 2007 | 16973731 |
| post-translational regulation of rotavirus protein nsp1 expression in mammalian cells. | the nonstructural rotavirus protein nsp1 binds specifically to viral mrnas and to interferon regulatory factor 3 (irf3), inducing irf3 degradation through a proteasome-dependent pathway. by using a vaccinia virus expression system in mammalian cells, we found that the yield of nsp1 was 8- and 13-fold lower than the viral proteins vp2 or nsp3, respectively; while in the presence of proteasome inhibitors such difference could be reduced to 2- to 2.5-fold, respectively. the susceptibility of nsp1 t ... | 2007 | 17019530 |
| hsp70 negatively controls rotavirus protein bioavailability in caco-2 cells infected by the rotavirus rf strain. | previous studies demonstrated that the induction of the heat shock protein hsp70 in response to viral infection is highly specific and differs from one cell to another and for a given virus type. however, no clear consensus exists so far to explain the likely reasons for hsp70 induction within host cells during viral infection. we show here that upon rotavirus infection of intestinal cells, hsp70 is indeed rapidly, specifically, and transiently induced. using small interfering rna-hsp70-transfec ... | 2007 | 17079279 |
| presence of viral proteins in drinkable water--sufficient condition to consider water a vector of viral transmission? | in order to determine the role of water as a possible vector for transmission of the most prevalent enteric viruses affecting infantile populations, 226 water samples were collected from facatativa's (colombian municipality located 30km away from bogotá) water works in the years 2000, 2002, and 2005. the samples were clarified and virus was concentrated by filtering and ultrafiltering techniques. the presence of viral protein (vp) was assessed by enzyme immunoassay method (eia) and viral rna pre ... | 2007 | 17084879 |
| procedure for rapid concentration and detection of enteric viruses from berries and vegetables. | several hepatitis a virus (hav) and norovirus (nv) outbreaks due to consumption of berries and vegetables have been reported during recent years. to facilitate the detection of enteric viruses that may be present on different fresh and frozen products, we developed a rapid and sensitive detection method for hav, nv, and rotavirus (rv). initial experiments focused on optimizing the composition of the elution buffer, improving the viral concentration method, and evaluating the performance of vario ... | 2007 | 17085706 |
| rotavirus impairs the biosynthesis of brush-border-associated dipeptidyl peptidase iv in human enterocyte-like caco-2/tc7 cells. | rotavirus is the leading cause of severe dehydrating diarrhoea in infants and young children worldwide. this virus infects mature enterocytes in the small intestine, and induces structural and functional damage. in the present study, we have identified a new mechanism by which rotavirus impairs a brush border-associated intestinal protein. we show that infection of enterocyte-like caco-2/tc7 cells by rhesus monkey rotavirus (rrv) impairs the biosynthesis of dipeptidyl peptidase iv (dpp iv), an i ... | 2007 | 17081193 |
| detection and characterization of human group c rotavirus in the pediatric population of barcelona, spain. | the role of group c rotavirus as a cause of childhood diarrhea is not well defined. | 2007 | 17070729 |
| evidence for independent segregation of the vp6- and nsp4- encoding genes in porcine group a rotavirus g6p[13] strains. | molecular characterization of two porcine group a rotavirus strains (hp113 and hp140), detected from eastern india, revealed a vp7 closely related to those of human g6p[14] strains, vp4 with a borderline p[13] genotype, and vp6 related to bovine and human sgi strains rather than porcine sgi and/or sgii group a rotaviruses. both strains had nsp4 and nsp5 of porcine origin. therefore, to our knowledge, the present study is the first report of detection of group a rotavirus strains with g6p[13] gen ... | 2007 | 17006597 |
| genetic analysis of an adrv-n-like novel rotavirus strain b219 detected in a sporadic case of adult diarrhea in bangladesh. | an unusual human rotavirus strain b219 was detected in a stool specimen from a 65-year old patient with diarrhea in bangladesh during april 2002. cloning and sequence analysis of five genes of the b219 strain indicated that this virus is genetically closely related to the adrv-n strain, which caused an adult diarrhea outbreak in china, but distinct from groups a, b, and c rotaviruses known to cause diarrheal diseases in humans. accordingly, rotavirus strains b219 and adrv-n were considered to be ... | 2007 | 16900303 |
| downstream processing of triple layered rotavirus like particles. | rotavirus like particles (rlps) constitute a potential vaccine for the prevention of rotavirus disease, responsible for the death of more than half a million children each year. increasing demands for pre-clinical trials material require the development of reproducible, scaleable and cost-effective purification strategies as alternatives to the traditional laboratory scale cscl density gradient ultracentrifugation methods commonly used for the purification of these complex particles. self-assemb ... | 2007 | 16959354 |
| rotavirus and not age determines gastroenteritis severity in children: a hospital-based study. | the severity of childhood gastroenteritis is generally believed to be age-related rather than aetiology-related. rotavirus-induced gastroenteritis is more severe than gastroenteritis caused by other enteric pathogens and is also age-related. we thus addressed the question of whether the increased severity of rotavirus-induced gastroenteritis is related to age or to features intrinsic to the agent. | 2007 | 16941130 |
| [epidemiological and virological aspects rotavirus diarrhoea in abidjan, côte d'ivoire (1997-2000)]. | viruses, mainly rotaviruses are aetiological agents in more than 80% of the cases of acute diarrhoea in children. in order to determine the epidemiological characteristics and genotypes of human rotaviruses involved in gastroenteritis in diarrheic children aged from 0 to 5 years old in abidjan, 642 specimens of stools were collected between 1997 and 2000 in the urban health centres and university teaching hospitals in abidjan. the antigenic detection of rotaviruses carried out by elisa test was ... | 2007 | 17982852 |
| amino acid domains 280-297 of vp6 and 531-554 of vp4 are implicated in heat shock cognate protein hsc70-mediated rotavirus infection. | the rotavirus infection mechanism seems to be a multi-step process which is still not fully understood. the heat shock cognate protein hsc70 has been proposed as being a co-receptor molecule for rotavirus entry into susceptible cells. in this work, an attempt was made to determine the existence of possible domains for vp4 and vp6 binding to hsc70. we selected amino acid sequences 531-554 from vp4 and 280-297 from vp6 on the basis of already recognized sequences for binding to hsc70. this study d ... | 2007 | 17876681 |
| [rotavirus vaccination in france: recommendations of the french speaking group of pediatric gastroenterology, hepatology and nutrition]. | the rotavirus is the major cause of infantile gastroenteritis worldwide. in europe and in industrialized countries, rotavirus infections bring out considerable economic expenses. in france, the burden of health expenses is particularly marked during winter period, the peak of rotavirus infections coinciding with that of the respiratory syncitial virus bronchiolitis, this specificity results in an increase of nosocomial infections during this period and a saturation of beds in paediatric hospital ... | 2007 | 17961815 |
| [administration of probiotic lactobacilli to children with gastrointestinal problems: there is still little evidence]. | probiotics are defined as 'live micro-organisms that confer a health benefit on the host if administered in adequate amounts'. there are only limited data in the literature regarding the efficacy and safety of lactobacilli for various gastrointestinal indications in children. lactobacillus rhamnosus gg (lgg) has no added value in the treatment of children with functional constipation. lactobacilli do seem to be effective in rotavirus diarrhoea. they seem safe and shorten the duration of the diar ... | 2007 | 17969569 |
| [rotavirus infections in a paediatric hospital during 5 years]. | rotavirus is the major cause of gastroenteritis in children and the main cause of hospital acquired-infection in paediatric unit. we report the epidemiology of gastroenteritis in our hospital during five consecutive years. rotavirus was involved in 13% of the patients. seasonal peaks were observed in january and 45.8% of the patients were less than 6 month old. the rotavirus infection was hospital-acquired in 1/3 of the cases. during the winter period, the incidence of rotavirus nosocomial infec ... | 2007 | 17977698 |
| [detection by rt-pcr of the 1st cases of astrovirus in human stools in abidjan, côte d'ivoire]. | viral gastroenteritis are a problem of public health because of the high rate of morbidity and mortality, particularly in children. among the etiologic agents, human astroviruses are the third agents most often incriminated after rotaviruses and caliciviruses. symptoms of gastroenteritis caused by astroviruses are generally moderated compared with those observed with rotaviruses and rarely involve hospitalization. in sub-saharan africa, particularly in côte d'ivoire, the majority of viral gastro ... | 2007 | 17982851 |