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environmental conditions affecting exopolysaccharide production by pseudomonas aeruginosa, micrococcus sp., and ochrobactrum sp.three different chromium-resistant microorganisms (pseudomonas aeruginosa, micrococcus sp., and ochrobactrum sp.) were tested with regard to their eps production at different ph levels, temperatures, cr(vi) concentrations, and incubation periods. the optimum ph level was 7 for p. aeruginosa and micrococcus sp., while it was 8 for ochrobactrum sp. according to the highest eps amount at 100 mg/l cr(vi) concentration. the highest production of epss by the three bacteria was obtained under different ...200818155834
thermodynamics of the binding of cytotoxic protoberberine molecule coralyne to deoxyribonucleic acids.the binding thermodynamics of the interaction of protoberberine molecule coralyne to various dnas have been investigated. thermodynamic data revealed that the binding was enthalpy driven in gc rich dna and gc polynucleotides while the same was favored by both negative enthalpy and positive entropy changes in the at rich dna and at polymers. parsing the free energy change of the binding in terms of polyelectrolytic and nonpolyelectrolytic contribution showed the involvement of major contributions ...200818157954
uv protective effects of dna repair enzymes and rna lotion.solar uv radiation is known to cause immune suppression, believed to be a critical factor in cutaneous carcinogenesis. although the mechanism is not entirely understood, dna damage is clearly involved. sunscreens function by attenuating the uv radiation that reaches the epidermis. however, once dna damage ensues, repair mechanisms become essential for prevention of malignant transformation. dna repair enzymes have shown efficacy in reducing cutaneous neoplasms among xeroderma pigmentosum patient ...200818173718
cathepsin l and cystatin b gene expression discriminates immune coelomic cells in the leech theromyzon tessulatum.previous studies evidenced that cystatin b-like gene is specifically expressed and induced in large circulating coelomic cells following bacterial challenge in the leech theromyzon tessulatum. in order to understand the role of that cysteine proteinase inhibitor during immune response, we investigated the existence of members of cathepsin family. we cloned a cathepsin l-like gene and studied its tissue distribution. immunohistochemical studies using anti-cathepsin l and anti-cystatin b antibodie ...200818177937
charge-directed targeting of antimicrobial protein-nanoparticle conjugates.use of antimicrobial enzymes covalently attached to nanoparticles is of great interest as an antibiotic-free approach to treat microbial infections. intrinsic properties of nanoparticles can also be used to add functionality to their conjugates with biomolecules. here, we show in a model system that nanoparticle charge can be used to enhance delivery and increase bactericidal activity of an antimicrobial enzyme, lysozyme. hen egg lysozyme was covalently attached to two types of polystyrene latex ...200818183633
genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants.the genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in cuba was analysed by two primers-ramdom amplified polymorphic dna fingerprinting (tp-rapd) and 16s rrna gene sequencing. the strains were distributed into 17 groups on the basis of their tp-rapd patterns, and a representative strain from each group was subjected to 16s rrna gene sequencing. analysis of these sequences showed that the is ...200818383223
differences in the effects of solution additives on heat- and refolding-induced aggregation.although a number of low-molecular-weight additives have been developed to suppress protein aggregation, it is unclear whether these aggregation suppressors affect various aggregation processes in the same manner. in this study, we evaluated the differences in the effect of solution additives on heat- and refolding-induced aggregation in the presence of guanidine (gdn), arginine (arg), and spermidine (spd), and the comparable analysis showed the following differences: (i) gdn did not suppress th ...200818386919
protein recognition via ion-coordinated molecularly imprinted supermacroporous cryogels.molecular imprinting is a method for making selective binding sites in synthetic polymers using a molecular template. the aim of this study is to prepare lysozyme-imprinted supermacroporous cryogels which can be used for the purification of lysozyme (lyz) from egg white. n-methacryloyl-(l)-histidinemethylester (mah) was chosen as the metal-coordinating monomer. in the first step, cu2+ was complexed with mah and the lysozyme-imprinted poly(hema-mah) [lyz-mip] cryogel were produced by free radical ...200818395214
[bacteremia caused by kocuria kristinae in a patient with acute leukaemia]. 200818395377
purification of bacteriocin ls1 produced by human oral isolate lactobacillus salivarius bgho1.lactobacillus salivarius bgho1, a human oral isolate with antagonistic activity against growth of streptococcus mutans, streptococcus pneumoniae, staphylococcus aureus, enterococcus faecalis, micrococcus flavus, and salmonella enteritidis, probably produces more than one proteinaceous antimicrobial substance. the objective of this study was the purification of a bacteriocin, named ls1, produced by l. salivarius bgho1.200818402613
a randomized trial of 2% chlorhexidine tincture compared with 10% aqueous povidone-iodine for venipuncture site disinfection: effects on blood culture contamination rates.contaminated blood cultures have been recognized as a bothersome issue, and continue to cause frustration for clinicians. skin antiseptics can prevent blood culture contamination. to our knowledge, there have been no randomized studies to compare 2% alcoholic chlorhexidine and 10% aqueous povidone-iodine for venipuncture site disinfection.200818407355
prostaglandin e2 modulates the expression of antimicrobial peptides in the fat body and midgut of anopheles albimanus.prostaglandins (pgs) participate in the regulation of vertebrate and in at least six insect orders' immune responses. we identified pge2 in midgut, fat body, malpighian tubules, and ovarioles of anopheles albimanus (aa) mosquitoes. our data indicate that pge2 synthesis in cultured midguts responds to the presence of two bacterial species, micrococcus luteus and klebsiella pneumoniae. the production of mrna coding for antimicrobial peptides aa-attacin, aa-cecropin, and aa-gambicin was observed in ...200818412259
interaction of isoquinoline alkaloid palmatine with deoxyribonucleic acids: binding heterogeneity, and conformational and thermodynamic aspects.the binding heterogeneity, conformational aspects, and energetics of the interaction of the cytotoxic plant alkaloid palmatine have been studied with various natural and synthetic dnas. the alkaloid binds to calf thymus and escherichia coli dna that have mixed at and gc sequences in almost equal proportions with positive cooperativity, while, with clostridium perfringens and micrococcus lysodeikticus dna with predominantly high at and gc sequences, respectively, noncooperative binding was observ ...200818421749
nagelamides k and l, dimeric bromopyrrole alkaloids from sponge agelas species.two new dimeric bromopyrrole alkaloids, nagelamides k (1) and l (2), have been isolated from okinawan marine sponges agelas species, and the structures and stereochemistry were elucidated from spectroscopic data. nagelamide k (1) is a bromopyrrole alkaloid possessing a rare piperidinoiminoimidazolone ring with an aminoimidazole ring and a taurine unit, while nagelamide l (2) is a unique dimeric bromopyrrole alkaloid containing an ester linkage. nagelamides k (1) and l (2) exhibited antimicrobial ...200818454539
lineage-specific transition of histone signatures in the killer cell ig-like receptor locus from hematopoietic progenitor to nk cells.the clonal distribution and stable expression of killer cell ig-like receptor (kir) genes is epigenetically regulated. to assess the epigenetic changes that occur during hemopoietic development we examined dna methylation and chromatin structure of the kir locus in early hemopoietic progenitor cells and major lymphocyte lineages. in hemopoietic progenitor cells, kir genes exhibited the major hallmarks of epigenetic repression, which are dense dna methylation, inaccessibility of chromatin to micr ...200818097043
successful control of aggregation and folding rates during refolding of denatured lysozyme by adding n-methylimidazolium cations with various n'-substituents.the present study aimed to obtain more effective refolding agents and to understand the influence of their chemical structures on their function as refolding agents. to achieve these aims, we investigated the effects of a large variety of n'-substituted n-methylimidazolium chlorides on the oxidative refolding of lysozyme in a high throughput manner. among the molecules examined, n-methylimidazolium cations with a short n'-alkyl chain, such as an n'-ethyl or n'-butyl chain, significantly enhanced ...200818197673
assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches.microbial community structure of two distinct effluent treatment plants (etps) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16s rrna gene sequencing, (ii) diversity index analysis - operational taxonomic units (otus). a total of 38 and 44 bacterial otus having 85-99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. fift ...200818280146
molecular cloning, characterization and expression of heat shock protein 90 gene in the haemocytes of bay scallop argopecten irradians.heat shock protein 90 (hsp90) is a highly conserved molecular chaperone that plays key roles in the folding, maintenance of structural integrity and regulation of a subset of cytosolic proteins. in the present study, the cdna of argopecten irradians hsp90 (designated aihsp90) was cloned by the combination of homology cloning and rapid amplification of cdna ends (race) approaches. the full-length cdna of aihsp90 was of 2669 bp, including an open reading frame (orf) of 2175 bp encoding a polypepti ...200818282767
the effect of various inflammatory agents on the phagocytosis and cytokine profile of mouse and rat macrophages.the effects of various inflammatory stimuli on the cytokine profile and phagocytic capacity of mouse and rat peritoneal macrophages were investigated in vitro. the correlations between cytokine concentrations and the expressions of nos ii and arginase were also studied.200818288457
nitrate removal efficiency of bacterial consortium (pseudomonas sp. kw1 and bacillus sp. yw4) in synthetic nitrate-rich water.the efficiency of bacterial isolates to reduce nitrate from synthetic nitrate-rich water was tested using a batch scale process. two efficient nitrate reducing bacterial species were isolated from water samples collected from kodaikanal and yercaud lakes. bacterial analysis of the samples revealed the presence of nitrate reducing bacteria belonging to the genera pseudomonas, bacillus, micrococcus and alcaligenes. among the isolates, the consortium of pseudomonas sp. kw1 and bacillus sp. yw4 was ...200818294766
nmr based structure-activity relationship analysis of an antimicrobial peptide, thanatin, engineered by site-specific chemical modification: activity improvement and spectrum alteration.activity improvement of an antimicrobial peptide, thanatin, has been achieved up to 4-fold higher than natural original one by site-specific chemical modifications with tert-butyl group at two cysteine residues which form an intramoleular disulfide bridge. the chemically modified thanatin (c11tbu/c18tbu) exhibited improved antimicrobial activity toward gram-positive bacteria, micrococcus luteus, whereas lowered activity toward gram-negative bacteria, escherichia coli. this finding suggests that ...200818294955
synthesis, structure and biological activities of cobalt(ii) and zinc(ii) coordination compounds with 2-benzimidazole derivatives.in this work we present the synthesis, structural and spectroscopic characterisation of a series of cobalt(ii) and zinc(ii) coordination compounds with benzimidazole (bz) and its 2-benzimidazole derivatives: 2-aminobenzimidazole (2ab), albendazole (abz) and tris(2-benzimidazolylmethyl)amine (ntb). the compounds were evaluated for their in vitro antimicrobial activity against staphylococcus aureus, micrococcus luteus, salmonella typhi, pseudomonas aeruginosa, escherichia coli and proteus vulgaris ...200818304644
monitoring microbial populations on wide-body commercial passenger aircraft.although exposure to bacteria has been assessed in cabin air previously, minimal numbers of samples have been collected in-flight. the purpose of this research was to comprehensively characterize bacterial concentrations in the aircraft cabin. twelve randomly selected flights were sampled on boeing-767 aircraft, each with a flight duration between 4.5 and 6.5 h. n-6 impactors were used to collect sequential, triplicate air samples in the front and rear of coach class during six sampling interval ...200818316352
case of the month. pitted keratolysis. 200818323358
kinetic studies of micrococcus luteus b-p 26 undecaprenyl diphosphate synthase reaction using 3-desmethyl allylic substrate analogs.in order to investigate the substrate binding feature of undecaprenyl diphosphate synthase from micrococcus luteus b-p 26 with respect to farnesyl diphosphate and a reaction intermediate, (z,e,e)-geranylgeranyl diphosphate, we examined the reactivity of artificial substrate analogs, 3-desmethyl farnesyl diphosphate and 3-desmethyl z-geranylgeranyl diphosphate, which lack the methyl group at the 3-position of farnesyl diphosphate and z-geranylgeranyl diphosphate, respectively. undecaprenyl diphos ...200818323637
antibacterial activity of dextran-conjugated lysozyme against escherichia coli and staphylococcus aureus in cheese curd.the purposes of this research were to glycosylate lysozyme with dextran under maillard reaction conditions and assess the antimicrobial characteristics of the lysozyme-dextran conjugate in a culture medium and cheese curd. solutions containing lysozyme and dextran were incubated at 60 degrees c and at 79% relative humidity. gel permeation chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis were used to follow the glycosylation process. under optimum conditions 3.7 mol of ...200818326197
occurrence of resistance to antibiotics, uv-b, and arsenic in bacteria isolated from extreme environments in high-altitude (above 4400 m) andean wetlands.high-altitude andean wetlands are pristine environments with extreme conditions such as high uv radiation, high heavy metal content (mainly arsenic), high salinity, and oligotrophy. in this paper, the uv-b resistance and tolerance to arsenic of phylogenetically characterized bacteria (actinobacteria [six isolates], firmicutes [four isolates], and gamma-proteobacteria [three isolates]) isolated from laguna vilama (4400-m altitude) and laguna azul (4560 m) were determined. in addition, given that ...200818330637
bovine hemoglobin: an attractive source of antibacterial peptides.a peptic hemoglobin hydrolysate was fractioned by a semi-preparative reversed-phase hplc and some fractions have an antibacterial activity against four bacteria strains: micrococcus luteus a270, listeria innocua, escherichia coli and salmonella enteritidis. these fractions were analyzed by esi/ms and esi/ms/ms, in order to characterize the peptides in these fractions. each fraction contains at least three peptides and some fractions contain five peptides. all these fractions were purified severa ...200818342399
digestive function of lysozyme in synanthropic acaridid mites enables utilization of bacteria as a food source.the activity of lysozyme, the enzyme that hydrolyzes peptidoglycan in g(+) bacterial cell walls, was detected in whole mite extracts (wme) and in spent growth medium extracts (sgme) of 14 species of synanthropic mites (acari: acaridida). the adaptation of lysozyme for digestive activity and bacteriophagy was based on: (i) high lysozyme activity in sgme, and (ii) the correlation of maximum lysozyme activity at acidic ph values, corresponding to ph in the ventriculus and caeca. we show that the di ...200818357505
cloth colorization caused by microbial biofilm.in this study, cloth disfeaturement was investigated biologically. to clarify whether or not microbes can cause cloth disfeaturement, and to identify the microbes causing the disfeaturement, worn cloth samples were incubated on sweat-ingredient agar medium. non-sterilized cloth samples became yellow-colored during incubation, and bacterial strains belonging to the genera bacillus, brevibacterium, kocuria, micrococcus and staphylococcus were isolated from the yellow-colored parts. two major isola ...200818359206
antimicrobial activity of miconia species (melastomataceae).this work evaluated the antimicrobial activity of the methanol and chloroform extracts of the leaves of miconia cabucu, miconia rubiginosa, and miconia stenostachya using the disc-diffusion method. the results obtained showed that the methanol extracts of the leaves of m. rubiginosa and m. stenostachya and the chloroform extract of the leaves of m. cabucu presented antimicrobial activity against the tested microorganisms.200818361747
[study of ectoparasitism of ultramicrobacteria of the genus kaistia, strains nf1 and nf3 by electron and fluorescence microscopy].transmission electron and fluorescence microscopy was used to study the character of the interaction of free-living ultramicrobacterial (umb) strains nf1 and nf3, affiliated with the genus kaistia, and seven species of gram-positive and gram-negative heterotrophic bacteria. strains nf1 and nf3 were found to exhibit parasitic activity against gram-positive bacillus subtilis and gram-negative acidovorax delafildii. umb cells are tightly attached to the envelopes of the victim cells and induce thei ...200818365722
[effect of bacterial satellites on chlamydomonas reinhardtii growth in an algo-bacterial community].the growth characteristics of an algo-bacterial community (chlamydomonas reinhardtii and bacterial satellites) were studied, as well as the mechanism and patterns of bacterial effect on algae. four strains of predominant bacteria were isolated and partially characterized. they were assigned to the following taxa: rhodococcus terrea, micrococcus roseus, and bacillus spp. a pure culture of the alga under study was obtained by plating serial dilutions on agarized media with ampicillin. within the a ...200818365726
[role of the clasping-leaved pondweed (potamogeton perfoliatus) polysaccharides in formation of its bacterial environment].the effect of the polysaccharides of clasping-leaved pondweed (potamogeton perfioliatus) on the formation of a bacteriocenosis of this plant was demonstrated by research on chemoreception, relative surface hydrophobicity, and the growth characteristics of the members of five bacterial genera abundant in this microbiocenosis. the plant heteropolysaccharides of anionic and cationic nature were found to participate in selective stimulation or inhibition of growth of some microbial groups in surroun ...200818365727
[expression in e.coli and bioactivity assay of micrococcus luteus resuscitation promoting factor domain and its mutants].to express micrococcus luteus resuscitation promoting factor (rpf) domain and its mutants in prokaryotic cells, and to investigate their bioactivity.200819080603
cloning, purification and comparative characterization of two digestive lysozymes from musca domestica larvae.cdna coding for two digestive lysozymes (mdl1 and mdl2) of the musca domestica housefly was cloned and sequenced. mdl2 is a novel minor lysozyme, whereas mdl1 is the major lysozyme thus far purified from m. domestica midgut. mdl1 and mdl2 were expressed as recombinant proteins in pichia pastoris, purified and characterized. the lytic activities of mdl1 and mdl2 upon micrococcus lysodeikticus have an acidic ph optimum (4.8) at low ionic strength (mu = 0.02), which shifts towards an even more acid ...200819099150
renaturation of lysozyme with a protein disulfide isomerase chaperone results in enzyme super activity.when the oxidative refolding of lysozyme (lyzm) was carried out in the presence of protein disulfide isomerase (pdi) an increased refolding rate and a recovered activity exceeding 100% were reproducibly observed. the origin of this excess activity was investigated by hplc, sds-page, and mass spectrometry and assessed using an assay for lyzm activity. the refolding of lyzm was achieved through the formation of pdi-lyzm intermediates and the excess activity was derived from the nascent lysozyme re ...200819111648
escravos light crude oil degrading potentials of axenic and mixed bacterial cultures.we assessed the relationship between growth profile and the extent of biodegradation of escravos light crude oil by axenic and mixed bacteria cultures in a shake flask. eleven petroleum-degrading bacteria were isolated by enrichment from oil-contaminated soils including, pseudomonas effusa, pseudomonas fluorescens, pseudomonas cruciviae, arthrobacter tumescens, pseudomonas species, pseudomonas tralucida, alcaligenes metacaligenes, micrococcus colpogenes, bacillus badius, nocardia paraffinae and ...200819029769
the capybara eye: clinical tests, anatomic and biometric features.to carry out a descriptive investigation of the capybara (hydrochaeris hydrochaeris) eye and to perform selected ophthalmic diagnostic tests with the aim of establishing normal physiological reference values for this species.200819046280
pitfalls in efficacy testing--how important is the validation of neutralization of chlorhexidine digluconate?effective neutralization of active agents is essential to obtain valid efficacy results, especially when non-volatile active agents like chlorhexidine digluconate (chg) are tested. the aim of this study was to determine an effective and non-toxic neutralizing mixture for a propan-1-ol solution containing 2% chg.200819046465
synthesis and antimicrobial activity of some novel oxadiazole derivatives.a series of 5-(3'-oxo-6'-(substituted aryl)-2',3',4',5'-tetrahydropyridazin-2'-ylmethyl )-2-substituted 1,3,4-oxadiazole has been synthesized. appropriate aromatic hydrocarbon reacts with succinic anhydride in the presence of aicl3 to yield beta-aroyl propionic acid (1a). the corresponding acid is cyclized with hydrazine hydrate to give 6-(substituted aryl)-2,3,4,5-tetrahydro-3-pyridazinone (1b). this ntermediate after reaction with ethyl bromoacetate, was hydrazinolyzed into 3-oxo-6-(substitute ...200819051585
antimicrobial activity of iodoquinol 1%-hydrocortisone acetate 2% gel against ciclopirox and clotrimazole.commercially available topical formulations consisting of iodoquinol 1%-hydrocortisone acetate 2%, ciclopirox 0.77%, and clotrimazole 1%-betamethasone dipropionate 0.5% were assessed for their antimicrobial activity against cultures of micrococcus luteus, propionibacterium acnes, methicillin-resistant staphylococcus aureus (mrsa), pseudomonas aeruginosa, corynebacterium aquaticum, trichophyton mentagrophytes, malassezia furfur, microsporum canis, candida albicans, trichophyton rubrum, or epiderm ...200819055171
antimicrobial and antifungal activities of the extracts and essential oils of bidens tripartita.the aim of this study was to determine the antibacterial and antifungal properties of the extracts, subextracts and essential oils of bidens tripartita flowers and herbs. in the study, twelve extracts and two essential oils were investigated for activity against different gram-positive bacillus subtilis, micrococcus luteus, staphylococcus aureus, gram-negative bacteria escherichia coli, e. coli (beta-laktamase+), klebsiella pneumoniae (esbl+), pseudomonas aeruginosa and some fungal organisms can ...200819056546
antimicrobial activity of endodontic sealers based on calcium hydroxide and mta.the aim of this study was to evaluate the antimicrobial activity of a new root canal sealer containing calcium hydroxide (acroseal) and the root canal sealer based on mta (endo cpm sealer), in comparison with traditional sealers (sealapex, sealer 26 and intrafill) and white mta-angelus, against five different microorganism strains. the materials and their components were evaluated after manipulation, employing the agar diffusion method. a base layer was made using müller-hinton agar (mh) and wel ...200819177851
[selection and kinetic mechanism of psychrotrophs in low temperature wastewater treatment].a study was carried out taking low temperature domestic wastewater as target in laboratory, the composition of bacteria in activated sludge was analyzed and the degradation kinetics of organics was investigated. six preponderant psychrotrophs were selected out, belonging to zoogloea, aeromonas, flavobacterium, micrococcus, bacillus and pseudomonaus, respectively. results show that ttc-dehydrogenase activity of the psychrotroph is 25.44 mg/(l x h) which is 20.5 times more than that of ordinary ac ...200819186826
possible involvement of an extracellular superoxide dismutase (soda) as a radical scavenger in poly(cis-1,4-isoprene) degradation.gordonia westfalica kb1 and gordonia polyisoprenivorans vh2 induce the formation of an extracellular superoxide dismutase (sod) during poly(cis-1,4-isoprene) degradation. to investigate the function of this enzyme in g. polyisoprenivorans vh2, the soda gene was disrupted. the mutants exhibited reduced growth in liquid mineral salt media containing poly(cis-1,4-isoprene) as the sole carbon and energy source, and no sod activity was detectable in the supernatants of the cultures. growth experiment ...200818952871
production, isolation and biological activity of nargenicin from nocardia sp. cs682.culture broth of an actinomycete isolate, nocardia sp. cs682 showed specifically higher antibacterial activity against methicillin resistant staphylococcus aureus (mrsa). purified substance from the organism, cs-682, which is active against mrsa and micrococcus leuteus, is a c(28)h(37)no(8) (m+h(+), observed: 516.83) and identified as an unusual macrolide antibiotic, nargenicin. the chemical structure of cs-682 was identified by ft-ir, (1)h-nmr, (13)c-nmr, and ((1)h-(1)h and (1)h-(13)h) cosy. th ...200818958426
[new amides of phenazine-1-carboxylic acid: antimicrobial activity and structure-activity relationship].complex investigation of new phenazine-1-carboxylic acid (pca-1) phenylamides allowed to reveal their ability for substantial growth retardation of three gram-positive bacterial strains--micrococcus sp., erysipelothrix rhusiopathiae and staphylococcus aureus. the strong inhibitory activity of pca-1 derivatives towards the rna synthesis in in vitro t7-rna-polymerase transcription system was also shown, and this property depended on concentration and structure of the tested compounds. the methods ...200818959039
[in vitro studies on antioxidant and antimicrobial activities of polysaccharide from lycoris aurea].to study the preliminary antioxidant and antimicrobial activities of polysaccharide extracted from lycoris aurea.200819230410
molecular characterization of early colonizer bacteria from wastes in a steel plant.forty-nine bacteria isolated from four newly-produced waste samples of a steel industry, which had a high content of cao, mgo, cr and p2o5, were characterized molecularly and phenotypically by susceptibility testing against heavy metals.200819241517
[experimental vaginal dysbiosis on the model of white laboratory mice].qualitative and quantitative composition of microbiocenosis of urogenital tract (ugt) of mice has been studied. when investigating ugt of mice in norm (n = 8), microorganisms of several genera with following occurrence frequency were detected: lactobacillus (100%), streptococcus (100%), staphylococcus (87.5%), micrococcus (12.5%), bacillus (12.5%), fusobacterium (87.5%), peptococcus (62.5%), peptostreptococcus (50%), bacteroides (100%) and representatives of enterobacteriaceae f (12.5%) family. ...200819351049
linear alkylbenzene sulfonate tolerance in bacteria isolated from sediment of tropical water bodies polluted with detergents.the discharge of untreated detergent-bearing waste introduces linear alklcylbenzene sulfonates (las) to the aquatic environment. the surfactant persists in some streams and rivers in nigeria, some is adsorbed to suspended materials and end in the sediment of the receiving water bodies. in this study, bacteria isolated from sediments of some tropical detergent-effluent-polluted streams were tested for tolerance to las using the media dilution technique. las-tolerance was indicated by growth of th ...200819419067
growth and biosurfactant synthesis by nigerian hydrocarbon-degrading estuarine bacteria.the ability of microorganisms to degrade petroleum hydrocarbons is important for finding an environmentally-friendly method to restoring contaminated environmental matrices. screening of hydrocarbon-utilizing and biosurfactant-producing abilities of organisms from an estuarine ecosystem in nigeria, africa, resulted in the isolation of five microbial strains identified as corynebacterium sp. ddv1, flavobacterium sp. ddv2, micrococcus roseus ddv3, pseudomonas aeruginosa ddv4 and saccharomyces cere ...200819419068
bacterial resistance to antibiotics in acne vulgaris: an in vitro study.acne vulgaris is one of the most common skin disorders in youth especially during the puberty.200819882009
[erythromycin ethylsuccinate obtaining possibilities].in this study we tried to improve the erythromycin ethylsuccinate obtaining, having in view to separate the erythromycin ester by crystallization in water.200820209795
physico-chemical and microbiological profiles of "salchichón" (spanish dry-fermented sausage) enriched with orange fiber.spanish dry-fermented sausages with three concentrations (0, 1 and 2%) of orange fiber (juice industry by-product) were prepared and studied. fermentation and dry-curing processes were followed by physico-chemical (ph and water activity), chemical (moisture, lactic acid and residual nitrite level) and microbiological (aerobic mesophilic bacteria, lactic acid bacteria, enterobacteriaceae, micrococcaceae and molds and yeasts counts) analysis. sensory analyses were conducted on the finished product ...200822063347
[correlations between the level of bacterial resistance and the rate of clinical efficiency in the therapy of community-acquired respiratory tract infections].the aim of the study is to evaluate the efficiency of the first-line antibiotic treatment of the community-acquired respiratory tract infections in a population of young adults from an urban setting and to establish the pattern of antibiotic resistance of the germs involved. the bacteria most frequently identified have been: s. pneumoniae, h. influenzae, m. catarrhalis, atypical agents also being suspected. antibiotic treatment has been chosen accordingly to the recent guidelines, total clinical ...200921495346
rice seeds as sources of endophytic bacteria.endophytic bacteria are considered to originate from the external environment. to examine the hypothesis that rice (oryza sativa, cultivar kinuhikari) seeds are a source of endophytic bacteria, we isolated endophytic bacteria from the shoots, remains of the seeds, and roots of rice seedlings that were aseptically cultivated in vitro from surface-disinfected seeds. of the various bacterial strains isolated, the closest relatives, identified by 16s rrna gene sequencing, were: bacillus firmus, b. f ...200921566368
a survey of veterinary hospitals in nigeria for the presence of some bacterial organisms of nosocomial and zoonotic potential.this study was undertaken to determine the type and estimate the prevalence of bacterial organisms on contact surfaces of five close-to-patient facilities in three veterinary health care settings within the sokoto metropolis of north-western nigeria. a total of 30 samples (10 from each setting) were collected and analysed using culture, microscopy and biochemical testing. bacterial species isolated from samples in this study included the following: bacillus sp. (27.3%), staphylococcus aureus (15 ...200920391375
bloodstream infections due to micrococcus spp and intravenous epoprostenol. 200919888847
[bacterial biofilms on pvc tubing's inner surface of hemodialysis water treatment system].to determine the morphology, bacteria and endotoxin content of biofilms on the inner surface of pvc tubes in hemodialysis water treatment system.200919893257
bacterial contamination of automotive fuels in a tropical region: the case of costa rica.microbial contamination of fuel has been the cause of several problems in transportation and storage of these products. due to the lack of previous studies related to these problems in costa rica, bacterial quality was evaluated biannually in automotive fuels stored in the four oil distribution facilities of the costa rican petroleum refinery (recope). in 12 oil storage tanks, for a total of 96 samples, mesophilic, heterotrophic aerobic/facultative counts (astm d6974-04) and identification of ba ...200919928449
16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens.a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ...200919937215
assessment of microbiological quality of sachet-packaged drinking water in western nigeria and its public health significance.to assess the microbiological quality of sachet-packaged drinking water in western nigeria and its impact on public health.200919880150
high prevalence of pseudomonas species in soil samples from ternate island-indonesia.in the present study, ten soil samples were examined and the ph of the soil was recorded. for bacterial isolation, a sterile nutrient and blood agars were used. gram stain and biochemical tests were done for identification. a total of 384 genus were isolated, 314 (81.8%) were identified as pseudomonas species of which 245 (78.0%) were pseudomonas aeruginosa, 42 (13.4%) were pseudomonas fluorescens, 13 (4.2%) were pseudomonas mallei, 10 (3.1%) were pseudomonas putida and 4 (1.3%) were pseudomonas ...200919947183
decolorization of anthraquinone vat blue 4 by the free cells of an autochthonous bacterium, bacillus subtilis.uncontaminated soil, vat blue 4 contaminated soil and vat blue 4 effluent were screened for heterotrophic bacterial population and the bacterial density were found to be 19.3 x 10(4) colony forming units (cfu)/gm, 5.5 x 10(4) cfu/gm and 1.1 x 10(4) cfu/ml respectively. student's 't' test analysis affirmed that significant variation prevailed between the three set of 't' tests conducted (p<0.001 to 0.002). the heterotrophic bacterial population of dye contaminated soil comprised of 32.5% of pseud ...200919955647
antimicrobial effect of silver-impregnated cellulose: potential for antimicrobial therapy.silver has long been known to have antimicrobial activity. to incorporate this property into multiple applications, a silver-impregnated cellulose (sic) with low cytotoxicity to human cells was developed. sic differs from other silver treatment methods in that the leaching of silver particles is non-existent and the release of ionic silver is highly controlled.200919961601
bioactive constituents of the stem bark of mitrephora glabra.bioactivity-guided fractionation of the stem bark of mitrephora glabra yielded nine compounds, comprising three ent-kaurenoids (1-3), five polyacetylenic acids/esters (4-8), and one aporphine alkaloid, liriodenine (9). the structures of the six new compounds (1-3, 5, 7, and 8) were determined by spectroscopic data interpretation. all compounds were evaluated for their inhibitory activities against a panel of cancer cell lines and a battery of microorganisms.200919874044
identification of airborne bacterial and fungal species in the clinical microbiology laboratory of a university teaching hospital employing ribosomal dna (rdna) pcr and gene sequencing techniques.universal or "broad-range" pcr-based ribosomal dna (rdna) was performed on a collection of 58 isolates (n = 30 bacteria + 28 fungi), originating from environmental air from several locations within a busy clinical microbiology laboratory, supporting a university teaching hospital. a total of 10 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 27/30 (90%) of total bacterial species, consisting of seven genera and include ...200920183192
production and characterization of biosurfactants produced by microorganisms isolated from milk factory wastewaters.biosurfactants or surface-active compounds are produced by microorganisms. these molecules reduce the surface tension of both aqueous solutions and hydrocarbon mixtures. in this study, the isolation and identification of biosurfactant-producing microorganisms were assessed. the characterization of biosurfactant produced by microorganisms isolated from milk factory wastewaters was investigated. for this purpose, five different microorganisms were isolated and identified. in order to determine the ...200920088204
violacein-producing collimonas sp. from the sea surface microlayer of costal waters in trøndelag, norway.a new strain belonging to the genus collimonas was isolated from the sea surface microlayer off the coast of trøndelag, norway. the bacterium, designated collimonas ct, produced an antibacterial compound active against micrococcus luteus. subsequent studies using lc-ms identified this antibacterial compound as violacein, known to be produced by several marine-derived bacteria. fragments of the violacein biosynthesis genes vioa and viob were amplified by pcr from the collimonas ct genome and sequ ...200920098599
comparative analysis of micrococcus luteus isolates from blood cultures of patients with pulmonary hypertension receiving epoprostenol continuous infusion.during the period 2002-2008, at the national cardiovascular center, osaka, 28 micrococcus luteus isolates and one kocuria spp. isolate were obtained from blood cultures of pulmonary hypertension (ph) patients who were receiving continuous infusion therapy with epoprostenol. pulsed-field gel electrophoresis patterns of the isolates were unrelated, suggesting that the infections had multiple origins. the preparation of epoprostenol solution by patients themselves was thought to be a risk factor.200920012737
finding of the low molecular weight inhibitors of resuscitation promoting factor enzymatic and resuscitation activity.resuscitation promoting factors (rpf) are secreted proteins involved in reactivation of dormant actinobacteria, including mycobacterium tuberculosis. they have been considered as prospective targets for the development of new anti-tuberculosis drugs preventing reactivation of dormant tubercle bacilli, generally associated with latent tuberculosis. however, no inhibitors of rpf activity have been reported so far. the goal of this study was to find low molecular weight compounds inhibiting the enz ...200920016836
comparative immunological analysis of innate immunity activation after oral administration of wheat fermented extract to teleost fish.intradermal or oral administration of lipopolysaccharide derived from pantoea agglomerans (ip-pa1) has shown prophylactic and antitumor effects without serious side-effects. while it is known that tumor necrosis factor (tnf)-alpha produced by activated macrophages plays an important role in the expression mechanism following intradermal administration, details of the mechanism after oral administration remain unclear. in this study, the activation of innate immunity using fish as an animal model ...200920032450
a polyphasic study on the species diversity of the sediment microbiota of lake hévíz.lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ...200920038486
risk reduction of adverse effects due to di-(2-ethylhexyl) phthalate (dehp) by utilizing microbial degradation.di-(2-ethylhexyl) phthalate (dehp), one of the major phthalates, was reported to be a suspected endocrine-disrupting chemical (edc) that might produce developmental or reproductive toxicities. therefore, much effort was undertaken to reduce the potential risk of adverse effects of dehp on humans by diminishing environmental exposure to this chemical. a bacterium was isolated from soil contaminated with dehp at a poly(vinyl chloride) (pvc) manufacturing site. biodegradation kinetic experiments on ...200920077210
synthesis, spectral characterization, in-vitro microbiological evaluation and cytotoxic activities of novel macrocyclic bis hydrazone.a macrocyclic hydrazone schiff base was synthesized by reacting 1,4-dicarbonyl phenyl dihydrazide with 2,6-diformyl-4-methyl phenol and a series of metal complexes with this new schiff base were synthesized by reaction with co(ii), ni(ii) and cu(ii) metal salts. the schiff base and its complexes have been characterized by elemental analyses, ir, (1)h nmr, uv-vis, fab mass, esr spectra, fluorescence, thermal, magnetic and molar conductance data. the analytical data reveal that the co(ii), ni(ii) ...200919419802
two thymosin-repeated molecules with structural and functional diversity coexist in chinese mitten crab eriocheir sinensis.recently, beta-thymosin-like proteins with multiple thymosin domains (defined as thymosin-repeated proteins) have been identified from invertebrate. in the present study, the cdnas of two thymosin-repeated proteins (designated estrp1 and estrp2) were cloned from chinese mitten crab by expressed sequence tags (est) techniques. blast analysis presented three and two thymosin domains in estrp1 and estrp2, respectively, with the identities amongst the five domains varying from 47% to 100%. both estr ...200919428488
micrococcus endophyticus sp. nov., isolated from surface-sterilized aquilaria sinensis roots.a gram-positive bacterial strain, designated yim 56238(t), was isolated from plant roots (aquilaria sinensis), and characterized by using a polyphasic approach. strain yim 56238(t) grew optimally at ph 7.0-8.0 and at 28 degrees c. analysis of the 16s rrna gene sequence of strain yim 56238(t) indicated that it belongs to the genus micrococcus. chemotaxonomic data strongly supported the classification of this strain within the genus micrococcus: the cell-wall peptidoglycan contained lysine, glutam ...200919406795
preparation and characterization of protein loaded microspheres based on a hydroxylated aliphatic polyester, poly(lactic-co-hydroxymethyl glycolic acid).the purpose of this study was to investigate the suitability of a novel hydroxylated aliphatic polyester, poly(lactic-co-hydroxymethyl glycolic acid) (plhmga), as controlled release system for pharmaceutical proteins. dextran blue (as a macromolecular model compound) and lysozyme-loaded plhmga and plga (control formulation) microspheres were prepared by a solvent evaporation technique. the dextran blue and lysozyme loaded plhmga microspheres prepared with 10% polymer solution showed, because of ...200919409427
composition and antimicrobial activity of marrubium incanum desr. (lamiaceae) essential oil.the essential oil from the aerial parts of marrubium incanum desr. (lamiaceae), obtained by hydrodistillation, was analyzed by gc and gc-ms. forty-six compounds were identified, representing 96.3% of the total oil. the main components of the oil were (e)-caryophyllene (27.0%), germacrene d (26.2%) and bicyclogermacrene (11.5%). the microbial growth inhibitory properties of the isolated essential oil were determined using the agar diffusion and broth microdilution method against seven bacterial s ...200919413128
hemolin expression in the silk glands of galleria mellonella in response to bacterial challenge and prior to cell disintegration.hemolin, a member of the immunoglobulin protein superfamily, functions in lepidoptera as an opsonin in defence against potential pathogens and seems to play a role in tissue morphogenesis. we show that hemolin gene is expressed in several organs of galleria mellonella larvae, including the nervous system and the silk glands. the expression in the silk glands of the wandering larvae and their isolated abdomens is enhanced within 6h after an injection of bacteria, lipopolysaccharides, or peptidogl ...200919414015
the antibacterial and antifungal activity of a soda-lime glass containing silver nanoparticles.the antibacterial and antifungal activity of a low melting point soda-lime glass powder containing silver nanoparticles has been studied. nano-ag sepiolite fibres containing monodispersed silver nanoparticles (d(50) approximately 11 +/- 9 nm) were used as the source of silver. this powder presents a high antibacterial (against gram-positive and gram-negative bacteria) as well as antifungal (against i. orientalis) activity. the observed high activity against yeast has been explained by considerin ...200919417439
micrococcus luteus and pseudomonas species as probiotics for promoting the growth performance and health of nile tilapia, oreochromis niloticus.micrococcus luteus and pseudomonas species were isolated from the gonads and intestine of nile tilapia, oreochromis niloticus. m. luteus and ps. species antagonized aeromonas hydrophila with inhibition zone of 4 and 9 cm diameter, respectively. both microorganisms were added to artificial basal diet with 30% crude protein to evaluate their efficacy on the growth-performance and survival rate, besides some blood-parameters and chemistry. two hundred and forty o. niloticus with average body-weight ...200919361560
discovery of anas platyrhynchos avian beta-defensin 2 (apl_avbd2) with antibacterial and chemotactic functions.the cationic, cysteine-rich peptides called beta-defensins play a major role in the innate immune response. here, we describe the identification and characterization of the duck beta-defensin-2 homologue, anas platyrhynchos avian beta-defensin 2 (apl_avbd2). the 195 base pair open reading frame (orf) of apl_avbd2 has 83% identity with gga_avbd2 (chicken) and 85% identity with mga_avbd2 (turkey) at nucleotide level. the gene corresponding to the coding region is comprised of three exons and two i ...200919362739
chemical composition and antimicrobial activity of the essential oil from chaerophyllum aureum l. (apiaceae).the essential oils of the aerial parts and fruits of chaerophyllum aureum l., collected from two mountains in serbia, were analyzed by gc and gc/ms. sabinene (18.5-31.6%), p-cymene (7.9-25.4%) and limonene (1.9-10.9%) were characterized as the main constituents. the oils were tested against six bacterial strains and one strain of yeast, candida albicans. the highest antimicrobial activity was observed against the gram-positive bacteria staphylococcus aureus, s. epidermidis and micrococcus luteus ...200919370887
ecofriendly degradation of sulfonated diazo dye c.i. reactive green 19a using micrococcus glutamicus ncim-2168.micrococcus glutamicus ncim-2168 exhibited complete decolorization and degradation of c.i. reactive green 19a (an initial concentration of 50 mg l(-1)) within 42 h at temperature 37 degrees c and ph 8, under static condition. extent of mineralization was determined with total organic carbon (toc) and chemical oxygen demand (cod) measurement, showing a satisfactory reduction of toc (72%) and cod (66%) within 42 h. enzyme studies shows involvement of oxidoreductive enzymes in decolorization/degrad ...200919375909
coaggregation by the freshwater bacterium sphingomonas natatoria alters dual-species biofilm formation.coaggregation is hypothesized to enhance freshwater biofilm development. to investigate this hypothesis, the ability of the coaggregating bacterium sphingomonas natatoria to form single- and dual-species biofilms was studied and compared to that of a naturally occurring spontaneous coaggregation-deficient variant. attachment assays using metabolically inactive cells were performed using epifluorescence and confocal laser scanning microscopy. under static and flowing conditions, coaggregating s. ...200919376917
[serum activity of chitotriosidase, lysozyme and cathepsin h in patients with lung cancer and patients with inflammatory exudate (preliminary report)].lung cancer remains the leading cause of cancer death over the world. although new diagnostic methods have been discovered, new biomarkers of the cancer are still under studying. a human chitinolytic enzyme called chitotriosidase hydrolyzes chitin and chitotrioside substrates. it is specifically expressed by activating macrophages and seems to play a role in the defense against chitinous human pathogens. recently it has been shown that chitotriosidase may also attend to the inflammatory process. ...200919388531
limazepines a-f, pyrrolo[1,4]benzodiazepine antibiotics from an indonesian micrococcus sp.in our screening of indonesian microorganisms for novel bioactive natural products we have isolated seven new compounds, designated as limazepines a, b1 and b2 (isolated as an isomeric mixture), c, d, e, and f, from the culture broth of micrococcus sp. strain icbb 8177. in addition, the known natural products prothracarcin and 7-o-succinylmacrolactin a, as well as two previously reported synthetic compounds, 2-amino-3-hydroxy-4-methoxybenzoic acid methyl ester and 4-ethylpyrrole-2-carboxaldehyde ...200919388705
functional differential immune responses of mytilus galloprovincialis to bacterial challenge.bivalves are filter-feeders that can accumulate large numbers of bacteria, in particular vibrio species; these can persist within bivalve tissues largely depending on their sensitivity to the hemolymph bactericidal activity. in this work, functional parameters of the hemolymph of mytilus galloprovincialis were evaluated in response to in vivo challenge with different bacteria (gram(-) vibrio anguillarum and v. splendidus, gram+ micrococcus lysodeikticus). mussels were injected with heat-killed b ...200919393331
survival of staphylococcus and other bacteria in skin prick test antigens solutions.skin prick tests are commonly used for human allergy testing and determination. because solutions are kept at 4 degrees c and patient's skin is cleaned, the same solutions are used on many patients with the same applicators without sterilization.200919243860
actinobacterial community dynamics in long term managed grasslands.palace leas, a long-term experiment at cockle park farm, northumberland, uk was established in winter 1896-1897 since when the 13 plots have received regular and virtually unchanged mineral fertiliser and farm yard manure inputs. fertilisers have had a profound impact on soil ph with the organically fertilised plots showing a significantly higher ph than those receiving mineral fertiliser where ammonium sulphate has led to soil acidification. here, we investigate the impact of organic and minera ...200919247797
use of a microchip electrophoresis system for estimation of bacterial phylogeny and analysis of no3- reducing bacterial flora in field soils.phylogenetic estimation method without determination of dna sequence was developed. by this method, fragment length polymorphism separately digested with multiple restriction enzymes was measured using microchip electrophoresis and affiliated with those calculated from corresponding dna sequence in the theoretical database. the phylogenies of 129 no(3)(-) reducing bacteria newly isolated from field soils were estimated by this method, and were compared to those by carbon source utilization profi ...200919270404
compound-specific nitrogen isotope analysis of d-alanine, l-alanine, and valine: application of diastereomer separation to delta15n and microbial peptidoglycan studies.we have developed an analytical method to determine the compound-specific nitrogen isotope compositions of individual amino acid enantiomers using gas chromatography/combustion/isotope ratio mass spectrometry. a novel derivatization of amino acid diastereomers by optically active (r)-(-)-2-butanol or (s)-(+)-2-butanol offers two advantages for nitrogen isotope analysis. first, chromatographic chiral separation can be achieved without the use of chiral stationary-phase columns. second, the elutio ...200919061396
exposure to culturable airborne bioaerosols during noodle manufacturing in central taiwan.biological hazards associated with the manufacturing of noodles have not been well characterized in taiwan. this is an issue that flour workers can be exposed to bioaerosols (airborne fungi and bacteria) resulting flour-induced occupational asthma or allergic diseases. this study is to survey the species and concentrations of bioaerosols at different sites within a noodle factory for one year, and to investigate the effects of environmental factors on concentrations of bioaerosols. air samples w ...200919062076
proliferation of antibiotic-producing bacteria and concomitant antibiotic production as the basis for the antibiotic activity of jordan's red soils.anecdotes, both historical and recent, recount the curing of skin infections, including diaper rash, by using red soils from the hashemite kingdom of jordan. following inoculation of red soils isolated from geographically separate areas of jordan, micrococcus luteus and staphylococcus aureus were rapidly killed. over the 3-week incubation period, the number of specific types of antibiotic-producing bacteria increased, and high antimicrobial activity (mic, approximately 10 microg/ml) was observed ...200919286796
antibiotic susceptibility pattern of staphylococcus species isolated from telephone receivers.microorganisms are transferred to everyday objects from the environment and infected individuals. pathogenic microbes are transmissible from the air, skin, hands and other interpersonal contacts and cause diseases most of the time. this study centres on the microbial assessment of telephone receivers, their ability to transfer bacterial infections and the isolation of these microorganisms from receivers, with an emphasis on staphylococcus aureus and its antibiotic susceptibility pattern.200919296038
nagelamides q and r, novel dimeric bromopyrrole alkaloids from sponges agelas sp.two new dimeric bromopyrrole alkaloids, nagelamides q (1) and r (2), have been isolated from okinawan marine sponges of the genus agelas (ss-1134 and ss-956, respectively), and the structures and stereochemistry were elucidated from spectroscopic data. nagelamide q (1) is a rare dimeric bromopyrrole alkaloid possessing a pyrrolidine ring, while nagelamide r (2) is the first bromopyrrole alkaloid having an oxazoline ring. nagelamides q (1) and r (2) showed antimicrobial activity.200919296638
leptoglycin: a new glycine/leucine-rich antimicrobial peptide isolated from the skin secretion of the south american frog leptodactylus pentadactylus (leptodactylidae).antimicrobial peptides are components of innate immunity that is the first-line defense against invading pathogens for a wide range of organisms. here, we describe the isolation, biological characterization and amino acid sequencing of a novel neutral glycine/leucine-rich antimicrobial peptide from skin secretion of leptodactylus pentadactylus named leptoglycin. the amino acid sequence of the peptide purified by rp-hplc (c(18) column) was deduced by mass spectrometric de novo sequencing and conf ...200919298834
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