Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| the molecular mechanism of mitochondrial fusion. | this review is focused on mitochondrial membrane fusion, which is a highly conserved process from yeast to human cells. we present observations from both yeast and mammalian cells that have provided insights into the mechanism of mitochondrial fusion and speculate on how the key players, which are dynamin-related gtpases do the work of membrane tethering and fusion. | 2009 | 18691613 |
| monitoring the human beta1, beta2, beta3 adrenergic receptors expression and purification in pichia pastoris using the fluorescence properties of the enhanced green fluorescent protein. | the three beta adrenergic receptor subtypes, beta1-, beta2- and beta3-, were expressed in the methylotrophic yeast pichia pastoris. these receptors were n-terminally fused to the enhanced green fluorescent protein (egfp) and the fluorescent properties of egfp were used: (1) to select the recombinant strains, (2) to monitor the expression of the fluorescent receptors, and (3) to monitor the purification of the receptors by immobilized metal affinity chromatography. we demonstrate here that pichia ... | 2009 | 18797996 |
| single-chain intracellular antibodies inhibit influenza virus replication by disrupting interaction of proteins involved in viral replication and transcription. | influenza a virus is responsible for influenza epidemics in avian and human populations and poses a great threat to human health. many researches have been focused on the prevention and treatment of influenza a virus infection. the nucleoprotein (np) of the virus is an important protein due to its ability to interact with a variety of viral and cellular factors and its role in the viral rna synthesis. in this study, we have used the influenza a virus nucleoprotein as target for anti-viral therap ... | 2009 | 18687409 |
| yeast-secreted recombinant extracellular domain of human cd105 antigen is able to bind tgf-beta type ii receptor in vitro. | human cd105 antigen, a type i integral membrane glycoprotein, is expressed as homodimer and oligomer by human endothelial cells, and forms a heteromeric association with tgf-beta signaling receptors i and ii. several mutations of cd105 antigen gene are involved in a vascular disorder known as hereditary hemorrhagic telangiectasia type 1. the proposed mechanism by which cd105 is involved in said disorder is haploinsufficiency. we report expression and characterization of human cd105 antigen extra ... | 2009 | 18649149 |
| first case of continuous ambulatory peritoneal dialysis peritonitis due to candida sake. | fungal peritonitis is a relatively uncommon complication of peritoneal dialysis that contributes significantly to morbidity, drop out from the continuous ambulatory peritoneal dialysis (capd) program, and mortality. candida sake infections were rarely published in literature. we present the first case of peritonitis due to c. sake. a 41-year-old man was admitted to our hospital with abdominal pain, nausea, vomiting, fever, weakness. abdominal ultrasonography demonstrated a fistula tract, which h ... | 2009 | 18627471 |
| 80k-h interacts with inositol 1,4,5-trisphosphate (ip3) receptors and regulates ip3-induced calcium release activity. | inositol 1,4,5-trisphosphate receptors (ip3rs) are intracellular channel proteins that mediate calcium (ca2+) release from the endoplasmic reticulum, and they are involved in many biological processes (e.g. fertilization, secretion, and synaptic plasticity). recent reports show that ip3r activity is strictly regulated by several interacting molecules (e.g. ip3r binding protein released with inositol 1,4,5-trisphosphate, huntingtin, presenilin, danger, and cytochrome c), and perturbation of this ... | 2009 | 18990696 |
| phosphorylation of cardiac troponin i by mammalian sterile 20-like kinase 1. | mst1 (mammalian sterile 20-like kinase 1) is a ubiquitously expressed serine/threonine kinase and its activation in the heart causes cardiomyocyte apoptosis and dilated cardiomyopathy. its myocardial substrates, however, remain unknown. in a yeast two-hybrid screen of a human heart cdna library with a dominant-negative mst1 (k59r) mutant used as bait, ctn [cardiac tn (troponin)] i was identified as an mst1-interacting protein. the interaction of ctni with mst1 was confirmed by co-immunoprecipita ... | 2009 | 18986304 |
| predicting protein post-translational modifications using meta-analysis of proteome scale data sets. | protein post-translational modifications are an important biological regulatory mechanism, and the rate of their discovery using high throughput techniques is rapidly increasingly. to make use of this wealth of sequence data, we introduce a new general strategy designed to predict a variety of post-translational modifications in several organisms. we used the motif-x program to determine phosphorylation motifs in yeast, fly, mouse, and man and lysine acetylation motifs in man. these motifs were ... | 2009 | 18974045 |
| differential activities of cellular and viral macro domain proteins in binding of adp-ribose metabolites. | macro domain is a highly conserved protein domain found in both eukaryotes and prokaryotes. macro domains are also encoded by a set of positive-strand rna viruses that replicate in the cytoplasm of animal cells, including coronaviruses and alphaviruses. the functions of the macro domain are poorly understood, but it has been suggested to be an adp-ribose-binding module. we have here characterized three novel human macro domain proteins that were found to reside either in the cytoplasm and nucleu ... | 2009 | 18983849 |
| excision of the oxidatively formed 5-hydroxyhydantoin and 5-hydroxy-5-methylhydantoin pyrimidine lesions by escherichia coli and saccharomyces cerevisiae dna n-glycosylases. | (5r) and (5s) diastereomers of 1-[2-deoxy-beta-d-erythro-pentofuranosyl]-5-hydroxyhydantoin (5-oh-dhyd) and 1-[2-deoxy-beta-d-erythro-pentofuranosyl]-5-hydroxy-5-methylhydantoin (5-oh-5-me-dhyd) are major oxidation products of 2'-deoxycytidine and thymidine respectively. if not repaired, when present in cellular dna, these base lesions may be processed by dna polymerases that induce mutagenic and cell lethality processes. | 2009 | 18983898 |
| ynk1, the yeast homolog of human metastasis suppressor nm23, is required for repair of uv radiation- and etoposide-induced dna damage. | in humans, nm23-h1 is a metastasis suppressor whose expression is reduced in metastatic melanoma and breast carcinoma cells, and which possesses the ability to inhibit metastatic growth without significant impact on the transformed phenotype. nm23-h1 exhibits three enzymatic activities in vitro, each with potential to maintain genomic stability, a 3'-5' exonuclease and two kinases, nucleoside diphosphate kinase (ndpk), and protein histidine kinase. herein we have investigated the potential contr ... | 2009 | 18983998 |
| large-scale evaluation of candidate genes identifies associations between dna repair and genomic maintenance and development of benzene hematotoxicity. | benzene is an established human hematotoxicant and leukemogen but its mechanism of action is unclear. to investigate the role of single-nucleotide polymorphisms (snps) on benzene-induced hematotoxicity, we analyzed 1395 snps in 411 genes using an illumina goldengate assay in 250 benzene-exposed workers and 140 unexposed controls. highly significant findings clustered in five genes (blm, tp53, rad51, wdr79 and wrn) that play a critical role in dna repair and genomic maintenance, and these regions ... | 2009 | 18978339 |
| bioremediation of nitroexplosive wastewater by an yeast isolate pichia sydowiorum mcm y-3 in fixed film bioreactor. | nitroexplosives are essential for security and defense of the nation and hence their production continues. their residues and transformed products, released in the environment are toxic to both terrestrial and aquatic life. this necessitates remediation of wastewaters containing such hazardous chemicals to reduce threat to human health and environment. bioremediation technologies using microorganisms become the present day choice. high melting explosive (hmx) is one of the nitroexplosives produc ... | 2009 | 18979124 |
| identification of a novel bursicon-regulated transcriptional regulator, md13379, in the house fly musca domestica. | bursicon is a neuropeptide that regulates cuticle sclerotization (hardening and tanning) and wing expansion in insects via a g-protein coupled receptor. the hormone consists of alpha and beta subunits. in the present study, we cloned bursicon alpha and beta genes in the house fly musca domestica using 3' and 5' race and expressed the recombinant bursicon (rbursicon) heterodimer in mammalian 293 cells and insect highfive(tm) cells. the rbursicon displayed a strong bursicon activity in the neck-li ... | 2009 | 18980233 |
| identification of protamine 1 as a novel cancer-testis antigen in early chronic lymphocytic leukaemia. | early chronic lymphocytic leukaemia (cll) is an ideal disease for immunotherapy. we previously showed that semg 1 is a cancer-testis (ct) antigen in cll. in this study, semg 1 was applied as the bait in a yeast two-hybrid system of a testicular cdna library. seven clones were isolated and protamine (prm) 1 was identified as a novel ct antigen in early cll. prm1 transcripts were detected in 11/41 (26.8%) patients. prm 1 protein was also expressed but heterogeneously within individual patients. of ... | 2009 | 19036087 |
| knockdown of p180 eliminates the terminal differentiation of a secretory cell line. | we have previously reported that the expression in yeast of an integral membrane protein (p180) of the endoplasmic reticulum (er), isolated for its ability to mediate ribosome binding, is capable of inducing new membrane biogenesis and an increase in secretory capacity. to demonstrate that p180 is necessary and sufficient for terminal differentiation and acquisition of a secretory phenotype in mammalian cells, we studied the differentiation of a secretory cell line where p180 levels had been sig ... | 2009 | 19037105 |
| cold induced botrytis cinerea enolase (bcenol-1) functions as a transcriptional regulator and is controlled by camp. | botrytis cinerea is a necrotrophic fungal plant pathogen that can survive, grow and infect crops under cold stress. in an attempt to understand the molecular mechanisms leading to cold tolerance of this phytopathogen, we identified an enolase, bcenol-1. bcenol-1 encodes a 48 kda protein that shows high identity to yeast, arabidopsis and human enolases (72, 63 and 63%, respectively). northern analysis confirms that an increase in transcript abundance of bcenol-1 was observed when b. cinerea mycel ... | 2009 | 19011901 |
| aggresome-forming ttrap mediates pro-apoptotic properties of parkinson's disease-associated dj-1 missense mutations. | mutations in park7 dj-1 have been associated with autosomal-recessive early-onset parkinson's disease (pd). this gene encodes for an atypical peroxiredoxin-like peroxidase that may act as a regulator of transcription and a redox-dependent chaperone. although large gene deletions have been associated with a loss-of-function phenotype, the pathogenic mechanism of several missense mutations is less clear. by performing a yeast two-hybrid screening from a human fetal brain library, we identified tra ... | 2009 | 19023331 |
| the fox1 ferroxidase of chlamydomonas reinhardtii: a new multicopper oxidase structural paradigm. | multicopper oxidases (mco) contain at least four copper atoms arrayed in three distinct ligand fields supported by two canonical structural features: (1) multiples of the cupredoxin fold and (2) four unique sequence elements that include the ten histidine and one cysteine ligands to the four copper atoms. ferroxidases are a subfamily of mco proteins that contain residues supporting a specific reactivity towards ferrous iron; these mcos play a vital role in iron metabolism in bacteria, algae, fun ... | 2009 | 19023602 |
| genetic polymorphism in long-lived people: cues for the presence of an insulin/igf-pathway-dependent network affecting human longevity. | longevity in yeast, nematodes, fruit flies and mice is affected by mutations in the insulin/igf-1 or homologous pathways. studies on long-living people revealed some associations between genetic variants of the insulin/igf-1 pathway and longevity. here, we review such investigations, and we will report human longevity association studies regarding the variability of genes which modulate lifespan in model organisms by interacting with the insulin/igf-1 pathway. these studies will be presented in ... | 2009 | 19027825 |
| the hect domain of trip12 ubiquitinates substrates of the ubiquitin fusion degradation pathway. | the ubiquitin fusion degradation (ufd) pathway is a proteolytic system conserved in yeast and mammals in which an uncleavable ubiquitin moiety linked to the n terminus of a protein functions as a degradation signal of the fusion protein. although key components of the ufd pathway in yeast have been identified, the e3 enzyme of the human ufd pathway has not been studied. in this work, we show that trip12 is the e3 enzyme of the human ufd pathway. thus, trip12 catalyzes in vitro ubiquitination of ... | 2009 | 19028681 |
| functionality and substrate specificity of human box h/aca guide rnas. | a large number of box h/aca rnas have been identified in human cells, and have been predicted to account for nearly all pseudouridylation sites in human rrnas. however, the function of these mammalian h/aca rnas in directing pseudouridylation has been verified experimentally in only two cases. in this study, we used three in vitro reconstitution systems, including yeast and mammalian systems, to test the function of seven h/aca rnas guiding16 pseudouridylation sites. our results verified 12 of t ... | 2009 | 19033376 |
| cloning and expression of a new recombinant thrombolytic and anthithrombotic agent - a staphylokinase variant. | to develop a more potent antithrombin agent with thrombolytic and antiplatelet properties, a new staphylokinase (sak) variant was constructed. the kringle 2 domain (k2) of tissue type-plasminogen activator (t-pa) containing a fibrin-specific binding site (i), the rgd sequence (arg-gly-asp) for the prevention of platelet aggregation (ii) and the antithrombotic agent - hirulog (iii) was assembled to the c-terminal part of recombinant staphylokinase (r-sak). cdna for the hybrid protein sak-rgd-k2-h ... | 2009 | 19018330 |
| scythe/bat3 regulates apoptotic cell death induced by papillomavirus binding factor in human osteosarcoma. | papillomavirus binding factor (pbf) was first identified as a transcription factor regulating the promoter activity of human papillomavirus. we previously demonstrated that pbf is an osteosarcoma-associated antigen and 92% of osteosarcoma tissues express pbf in the nucleus. moreover, pbf-positive osteosarcoma has a significantly poorer prognosis than that with negative expression of pbf. in the present study, we assessed the biological role of pbf in cell survival. overexpression of pbf induced ... | 2009 | 19018758 |
| malassezia furfur fingerprints as possible markers for human phylogeography. | malassezia furfur was the first species described within the cosmopolitan yeast genus malassezia, which now comprises 13 species. reported isolation rates of these species from healthy and diseased human skin show geographic variations. pcr-fingerprinting with the wild-type phage m13 primer (5'-gagggtggcggttct-3') was applied to investigate phylogeographic associations of m. furfur strains isolated from scandinavians residing permanently in greece, in comparison to clinical isolates from greek, ... | 2009 | 19020555 |
| mdr quinone oxidoreductases: the human and yeast zeta-crystallins. | the medium-chain dehydrogenase/reductase (mdr) superfamily can be divided into zn-containing and zn-lacking proteins. zn-containing mdrs are generally well-known enzymes, mostly acting as dehydrogenases. the non-zn mdr are much less studied, and classified in several families of nadp(h)-dependent reductases, including quinone oxidoreductases (qor). zeta-crystallins are the best studied group of qor, have a structural function in the lens of several mammals, exhibit ortho-quinone reductase activi ... | 2009 | 19007762 |
| sumoylation and de-sumoylation: wrestling with life's processes. | the small ubiquitin-like modifier (sumo) is a ubiquitin-like protein that covalently modifies a large number of cellular proteins. sumo modification has emerged as an important regulatory mechanism for protein function and localization. sumoylation is a dynamic process that is mediated by activating (e1), conjugating (e2), and ligating (e3) enzymes and readily reversed by a family of ubiquitin-like protein-specific proteases (ulp) in yeast and sentrin/sumo-specific proteases (senp) in human. thi ... | 2009 | 19008217 |
| alternative splicing in class v myosins determines association with rab10. | rab proteins influence vesicle trafficking pathways through the assembly of regulatory protein complexes. previous investigations have documented that rab11a and rab8a can interact with the tail region of myosin vb and regulate distinct trafficking pathways. we have now determined that a related rab protein, rab10, can interact with myosin va, myosin vb, and myosin vc. rab10 localized to a system of tubules and vesicles that have partially overlapping localization with rab8a. both rab8a and rab1 ... | 2009 | 19008234 |
| receptor for activated c-kinase 1, a novel binding partner of adiponectin receptor 1. | adiponectin is an adipose tissue derived hormone with anti-diabetic and insulin-sensitizing properties. two adiponectin receptors, adipor1 and adipor2, have recently been identified, yet the signaling pathways triggered through adiponectin receptors remain to be elucidated. using a yeast two-hybrid screen, we identified an adaptor protein, receptor for activated protein kinase c1 (rack1), as an interacting partner of human adipor1. rack1 was confirmed to interact with adipor1 by co-immunoprecipi ... | 2009 | 19010305 |
| sirt1 regulates the ribosomal dna locus: epigenetic candles twinkle longevity in the christmas tree. | ribosomal rna (rrna) genes arrange themselves in a tandem pattern in nucleolus and during the transcription of rrna genes, the elongating nascent rrna transcripts create a structure called christmas tree. rrna genes in the rdna locus can be either active or silent depending on the epigenetic regulation of the chromatin structure. yeast sir2 (silent information regulator 2) protein containing complexes can repress the recombination in the rdna locus and subsequently extend the replicative lifespa ... | 2009 | 19010308 |
| expression, purification and characterization of recombinant human beta-amyloid 1-42 in pichia pastoris. | the human peptide rha beta(1-42) was effectively produced through a novel expression system and purification procedure. the peptide rha beta(1-42) was successfully expressed in pichia pastoris, the methylotrophic yeast that has never been used as host. the cdna encoding full-length ha beta(1-42) was synthesized with yeast bias codons and cloned into the ppicz alpha a vector in frame with the yeast alpha-factor secretion signal under the transcriptional control of the aox1 promoter and integrated ... | 2009 | 18950715 |
| a multi-layer method to study genome-scale positions of nucleosomes. | the basic unit of eukaryotic chromatin is the nucleosome, consisting of about 150 bp of dna wrapped around a protein core made of histone proteins. nucleosomes position is modulated in vivo to regulate fundamental nuclear processes. to measure nucleosome positions on a genomic scale both theoretical and experimental approaches have been recently reported. we have developed a new method, multi-layer model (mlm), for the analysis of nucleosome position data obtained with microarray-based approach. ... | 2009 | 18951969 |
| pancreatic autoantibodies are associated with reactivity to microbial antibodies, penetrating disease behavior, perianal disease, and extraintestinal manifestations, but not with nod2/card15 or tlr4 genotype in a hungarian ibd cohort. | pancreatic autoantibodies (pab) and goblet cell autoantibodies (gab) are specific for crohn's disease (cd) and ulcerative colitis (uc), but the sensitivity alone is low. conventional antibodies and carbohydrates (glycans) are associated with disease phenotype and may be of diagnostic importance in inflammatory bowel disease (ibd). our aim was to determine the accuracy of pab and gab autoantibodies as well as to study relevant phenotype-serotype associations. | 2009 | 18972554 |
| modomics: a database of rna modification pathways. 2008 update. | modomics, a database devoted to the systems biology of rna modification, has been subjected to substantial improvements. it provides comprehensive information on the chemical structure of modified nucleosides, pathways of their biosynthesis, sequences of rnas containing these modifications and rna-modifying enzymes. modomics also provides cross-references to other databases and to literature. in addition to the previously available manually curated trna sequences from a few model organisms, we h ... | 2009 | 18854352 |
| antifungal activity and mode of action of silver nano-particles on candida albicans. | in this study, the antifungal effects of silver nano-particles (nano-ag) and their mode of action were investigated. nano-ag showed antifungal effects on fungi tested with low hemolytic effects against human erythrocytes. to elucidate the antifungal mode of action of nano-ag, flow cytometry analysis, a glucose-release test, transmission electron microscopy (tem) and the change in membrane dynamics using 1,6-diphenyl-1,3,5-hexatriene (dph), as a plasma membrane probe, were performed with candida ... | 2009 | 18769871 |
| shd1 is a novel cytokine-inducible, negative feedback regulator of stat5-dependent transcription. | stat5 is a critical mediator of a variety of cytokine signaling whose transcriptional activity is regulated by associating with various proteins. during a search for stat5-interacting proteins, we identified shd1, a mammalian homologue of yeast gene sac3, as a potential interacter. shd1 was localized in the nucleus, and induced by cytokines that activate stat5, such as erythropoietin, interleukin-2 (il-2), or il-3. shd1 interacted specifically with stat5a and stat5b, and interestingly, it specif ... | 2009 | 18838617 |
| the conserved translocase tim17 prevents mitochondrial dna loss. | maintenance of an intact mitochondrial genome is essential for oxidative phosphorylation in all eukaryotes. depletion of mitochondrial genome copy number can have severe pathological consequences due to loss of respiratory capacity. in saccharomyces cerevisiae, several bifunctional metabolic enzymes have been shown to be required for mitochondrial dna (mtdna) maintenance. for example, ilv5 is required for branched chain amino acid biosynthesis and mtdna stability. we have identified oxa1 and tim ... | 2009 | 18826960 |
| umbelliferone aminoalkyl derivatives as inhibitors of human oxidosqualene-lanosterol cyclase. | human and murine lanosterol synthases (ec 5.4.99.7) were studied as targets of a series of umbelliferone aminoalkyl derivatives previously tested as inhibitors of oxidosqualene cyclases from other eukaryotes. tests were carried out on cell cultures of human keratinocytes and mouse 3t3 fibroblasts incubated with radiolabeled acetate, and on homogenates prepared from yeast cells expressing human lanosterol synthase, incubated with radiolabeled oxidosqualene. in cell cultures of both human keratino ... | 2009 | 18830877 |
| suppression mechanisms of cox assembly defects in yeast and human: insights into the cox assembly process. | eukaryotic cytochrome c oxidase (cox) is the terminal enzyme of the mitochondrial respiratory chain. cox is a multimeric enzyme formed by subunits of dual genetic origin whose assembly is intricate and highly regulated. in addition to the structural subunits, a large number of accessory factors are required to build the holoenzyme. the function of these factors is required in all stages of the assembly process. they are relevant to human health because devastating human disorders have been assoc ... | 2009 | 18522805 |
| time-synchronized clustering of gene expression trajectories. | current clustering methods are routinely applied to gene expression time course data to find genes with similar activation patterns and ultimately to understand the dynamics of biological processes. as the dynamic unfolding of a biological process often involves the activation of genes at different rates, successful clustering in this context requires dealing with varying time and shape patterns simultaneously. this motivates the combination of a novel pairwise warping with a suitable clustering ... | 2009 | 18502728 |
| pulsed electromagnetic field at 9.71 ghz increase free radical production in yeast (saccharomyces cerevisiae). | potential human health hazards have been reported after exposure to electromagnetic fields at low power density. increased oxidative stress has been suggested as a potential mechanism involved in long-term effect of such exposure. in the present work, yeast cultures were exposed for 20 min to a 9.71 ghz pulsed electromagnetic field at specific absorption rates (sar) from 0.5 w/kg to 16 w/kg. oxidative perturbations were investigated using esr spin trapping experiments and their impacts on membra ... | 2009 | 18321660 |
| nucleosome positioning by genomic excluding-energy barriers. | recent genome-wide nucleosome mappings along with bioinformatics studies have confirmed that the dna sequence plays a more important role in the collective organization of nucleosomes in vivo than previously thought. yet in living cells, this organization also results from the action of various external factors like dna-binding proteins and chromatin remodelers. to decipher the code for intrinsic chromatin organization, there is thus a need for in vitro experiments to bridge the gap between comp ... | 2009 | 20018700 |
| [prerc formation in xenopus egg extract: beyond the identification of factors, toward the understanding of regulation systems]. | 2009 | 21089472 | |
| [replication fork stabilization by replication stress checkpoint control]. | 2009 | 21089479 | |
| the ras/camp/pka signaling pathway and virulence in candida albicans. | candidiasis is the most common cause of fungal infections, and the majority of these are caused by candida albicans. the protean pathogenic potential of c. albicans includes the capacity to infect diverse mucosal and epidermal surfaces as well as to disseminate via the bloodstream to internal organs, potentially causing system failure in cases of severe immunosuppression. many environmental niches in the host may be invaded by c. albicans through modulation of gene expression patterns while chan ... | 2009 | 19995187 |
| 2'-hydroxylation of genistein enhanced antioxidant and antiproliferative activities in mcf-7 human breast cancer cells. | bioconversion of the isoflavonoid genistein to 2'- hydroxygenistein (2'-hg) was performed using isoflavone 2'-hydroxylase (cyp81e1) heterologously expressed in yeast. a monohydroxylated product was analyzed by liquid chromatography-electrospray ionization-mass spectrometry (lc-esi-ms) and nmr spectrometry and was identified as 2'-hg. an initial bioconversion rate of 6% was increased up to 14% under optimized conditions. after recovery, the biological activity of 2'-hg was evaluated. bioconverted ... | 2009 | 19996686 |
| human synthetic lethal inference as potential anti-cancer target gene detection. | two genes are called synthetic lethal (sl) if mutation of either alone is not lethal, but mutation of both leads to death or a significant decrease in organism's fitness. the detection of sl gene pairs constitutes a promising alternative for anti-cancer therapy. as cancer cells exhibit a large number of mutations, the identification of these mutated genes' sl partners may provide specific anti-cancer drug candidates, with minor perturbations to the healthy cells. since existent sl data is mainly ... | 2009 | 20015360 |
| prevalence of candida dubliniensis among cancer patients in kuwait: a 5-year retrospective study. | summary despite close genetic and phenotypic relationship of candida dubliniensis with candida albicans, its role in human disease is mostly restricted to oral colonisation, particularly among hiv-infected patients. the prevalence of c. dubliniensis in association with other disease conditions has been infrequently reported. in this study, we present data on the prevalence of c. dubliniensis among yeast species isolated from cancer patients over a 5-year period. a total of 1445 yeast isolates re ... | 2009 | 20002881 |
| the unstructured c-terminal tail of the 9-1-1 clamp subunit ddc1 activates mec1/atr via two distinct mechanisms. | dna damage checkpoint pathways operate to prevent cell-cycle progression in response to dna damage and replication stress. in s. cerevisiae, mec1-ddc2 (human atr-atrip) is the principal checkpoint protein kinase. biochemical studies have identified two factors, the 9-1-1 checkpoint clamp and the dpb11/topbp1 replication protein, as potential activators of mec1/atr. here, we show that g1 phase checkpoint activation of mec1 is achieved by the ddc1 subunit of 9-1-1, while dpb11 is dispensable. howe ... | 2009 | 20005839 |
| wrn helicase defective in the premature aging disorder werner syndrome genetically interacts with topoisomerase 3 and restores the top3 slow growth phenotype of sgs1 top3. | werner syndrome (ws) is a premature aging disorder characterized by genomic instability. the wrn gene defective in ws encodes a protein with both helicase and exonuclease activities that interacts with proteins implicated in dna metabolism. to understand its genetic functions, we examined the ability of human wrn to rescue phenotypes associated with sgs1, the sole recq helicase in saccharomyces cerevisiae. wrn failed to rescue sgs1 sensitivity to the dna damaging agent methylmethane sulfonate or ... | 2009 | 20157511 |
| sirt6 stabilizes dna-dependent protein kinase at chromatin for dna double-strand break repair. | the sir2 chromatin regulatory factor links maintenance of genomic stability to life span extension in yeast. the mammalian sir2 family member sirt6 has been proposed to have analogous functions, because sirt6-deficiency leads to shortened life span and an aging-like degenerative phenotype in mice, and sirt6 knockout cells exhibit genomic instability and dna damage hypersensitivity. however, the molecular mechanisms underlying these defects are not fully understood. here, we show that sirt6 forms ... | 2009 | 20157594 |
| the role of mitochondrial network dynamics in the pathogenesis of charcot-marie-tooth disease. | mitochondrial dysfunction plays a relevant role in the pathogenesis of neurological and neuromuscular diseases. mitochondria may be involved as a primary defect of either the mtdna or nuclear genome encoded subunits of the respiratory chain. these organelles have also been directly involved in the pathogenesis of mendelian neurodegenerative disorders caused by mutations in nuclear-encoded proteins targeted to mitochondria, such as friedreich ataxia, hereditary spastic paraplegia, or some monogen ... | 2009 | 20225023 |
| [screening of proteins binding to fxr1p using yeast two-hybrid technique]. | to screen the proteins interacting with fxr1p for functional investigation of fxr1p. | 2009 | 20034884 |
| keratin 18 attenuates estrogen receptor alpha-mediated signaling by sequestering lrp16 in cytoplasm. | oncogenesis in breast cancer is often associated with excess estrogen receptor alpha(eralpha) activation and overexpression of its coactivators. lrp16 is both an eralpha target gene and an eralpha coactivator, and plays a crucial role in eralpha activation and proliferation of mcf-7 breast cancer cells. however, the regulation of the functional availability of this coactivator protein is not yet clear. | 2009 | 20035625 |
| ptdins4p recognition by vps74/golph3 links ptdins 4-kinase signaling to retrograde golgi trafficking. | targeting and retention of resident integral membrane proteins of the golgi apparatus underly the function of the golgi in glycoprotein and glycolipid processing and sorting. in yeast, steady-state golgi localization of multiple mannosyltransferases requires recognition of their cytosolic domains by the peripheral golgi membrane protein vps74, an orthologue of human golph3/gpp34/gmx33/midas (mitochondrial dna absence sensitive factor). we show that targeting of vps74 and golph3 to the golgi appa ... | 2009 | 20026658 |
| fatty acid synthase impacts the pathobiology of candida parapsilosis in vitro and during mammalian infection. | cytosolic fungal fatty acid synthase is composed of two subunits alpha and beta, which are encoded by fas1 and fas2 genes. in this study, the fas2 genes of the human pathogen candida parapsilosis were deleted using a modified sat1 flipper technique. cpfas2 was essential in media lacking exogenous fatty acids and the growth of fas2 disruptants (fas2 ko) was regulated by the supplementation of different long chain fatty acids, such as myristic acid (14ratio0), palmitic acid (16ratio0), and tween 8 ... | 2009 | 20027295 |
| characterization of a secreted aspartyl protease of the fungal pathogen paracoccidioides brasiliensis. | paracoccidioides brasiliensis is a thermally dimorphic fungus that causes paracoccidioidomycosis, a human systemic disease prevalent in latin america. proteases have been described as playing an important role in the host invasion process in many pathogenic microorganisms. here we describe the identification and characterization of a secreted aspartyl protease (pbsap), isolated from a cdna library constructed with rnas of mycelia transitioning to yeast cells. recombinant pbsap was produced in es ... | 2009 | 20028235 |
| predicting highly-connected hubs in protein interaction networks by qsar and biological data descriptors. | hub proteins (those engaged in most physical interactions in a protein interaction network (pin) have recently gained much research interest due to their essential role in mediating cellular processes and their potential therapeutic value. it is straightforward to identify hubs if the underlying pin is experimentally determined; however, theoretical hub prediction remains a very challenging task, as physicochemical properties that differentiate hubs from less connected proteins remain mostly unc ... | 2009 | 20198194 |
| identification of malassezia pachydermatis from healthy and diseased human skin. | malassezia pachydermatis is the only species in the genus malassezia that is classically considered to be zoophilic. this yeast is only occasionally isolated from human skin, although it has been found to cause septic epidemics, especially in neonates. the aim of our study was to investigate the prevalence of m. pachydermatis on the skin of patients with malassezia-associated diseases and of healthy subjects. one hundred and sixty skin scrapings from patients with pityriasis versicolor (pv), seb ... | 2009 | 20380109 |
| [metacaspases and their role in the life cycle of human protozoan parasites]. | metacaspases are caspase-related cysteine-proteases that are present in organisms devoid of caspases such as plants, yeast, and protozoan parasites. since caspases are important effector molecules in mammalian apoptosis, the possible role of metacaspases in programmed cell death has been evaluated in the organisms where they are expressed. in some species of the human protozoan parasites trypanosoma spp. and leishmania spp., metacaspases have been involved in programmed cell death, although a ro ... | 2009 | 20436999 |
| protein n-glycosylation of gastropods. | glycosylation plays an important role in several types of recognition processes associated with fertilisation and development, allergies, pathological events and cell death. whereas the amino acid sequence of a protein is fixed by the dna, the glycosylation abilities depend on enzymes and substrates currently present in the cell.during the last decades our knowledge on glycosylation - the structure of glycans as well as the corresponding biochemical pathways including the responsible enzymes - e ... | 2009 | 21686044 |
| detection of gene orthology from gene co-expression and protein interaction networks. | ortholog detection methods present a powerful approach for finding genes that participate in similar biological processes across different organisms, extending our understanding of interactions between genes across different pathways, and understanding the evolution of gene families. | 2010 | 20438654 |
| hormone activity of hydroxylated polybrominated diphenyl ethers on human thyroid receptor-beta: in vitro and in silico investigations. | hydroxylated polybrominated diphenyl ethers (ho-pbdes) may disrupt thyroid hormone status because of their structural similarity to thyroid hormone. however, the molecular mechanisms of interactions with thyroid hormone receptors (trs) are not fully understood. | 2010 | 20439171 |
| recombinant yeast screen for new inhibitors of human acetyl-coa carboxylase 2 identifies potential drugs to treat obesity. | acetyl-coa carboxylase (acc) is a key enzyme of fatty acid metabolism with multiple isozymes often expressed in different eukaryotic cellular compartments. acc-made malonyl-coa serves as a precursor for fatty acids; it also regulates fatty acid oxidation and feeding behavior in animals. acc provides an important target for new drugs to treat human diseases. we have developed an inexpensive nonradioactive high-throughput screening system to identify new acc inhibitors. the screen uses yeast gene- ... | 2010 | 20439761 |
| autologous extracellular cytochrome c is an endogenous ligand for leucine-rich alpha2-glycoprotein and beta-type phospholipase a2 inhibitor. | beta-type phospholipase a(2) inhibitory protein (plibeta) from the serum of the venomous snake gloydius brevicaudus neutralizes basic phospholipase a(2) (pla(2)) from its own venom, and it has 33% sequence homology with human leucine-rich alpha(2)-glycoprotein (lrg), which has been recently reported to bind cytochrome c (cyt c) (cummings, c., walder, j., treeful, a., and jemmerson, r. (2006) apoptosis 11, 1121-1129). in the present study, plibeta was found to bind cyt c. the interactions of lrg ... | 2010 | 20442399 |
| characterizing the interaction between the rab6 gtpase and mint3 via flow cytometry based fret analysis. | in extension to previously applied techniques like yeast two-hybrid and gst pull-down assays, we successfully established a facs-based fret analysis to investigate the interaction of the mint3 adaptor protein and the small rab gtpase rab6a in living mammalian cells. a mint3 mutant containing only the ptb domain (mint3delta6) is able to interact with the constitutively active form of rab6a. mint3delta4, a mutant lacking part of the ptb domain was unable to interact with rab6a in gst pull-down ana ... | 2010 | 20447381 |
| delineation of wrn helicase function with exo1 in the replicational stress response. | the wrn gene defective in the premature aging disorder werner syndrome encodes a helicase/exonuclease. we examined the ability of wrn to rescue dna damage sensitivity of a yeast mutant defective in the rad50 subunit of mre11-rad50-xrs2 nuclease complex implicated in homologous recombination repair. genetic studies revealed wrn operates in a yexo1-dependent pathway to rescue rad50 sensitivity to methylmethane sulfonate (mms). wrn helicase, but not exonuclease, is required for mms resistance. wrn ... | 2010 | 20447876 |
| chd8 interacts with chd7, a protein which is mutated in charge syndrome. | charge syndrome is an autosomal dominant disorder caused in about two-third of cases by mutations in the chd7 gene. for other genetic diseases e.g. hereditary spastic paraplegia, it was shown that interacting partners are involved in the underlying cause of the disease. these data encouraged us to search for chd7 binding partners by a yeast two-hybrid library screen and chd8 was identified as an interacting partner. the result was confirmed by a direct yeast two-hybrid analysis, co-immunoprecipi ... | 2010 | 20453063 |
| cloning and expression of functional full-length human tissue plasminogen activator in pichia pastoris. | human tissue plasminogen activator (t-pa) plays a pivotal role in the treatment of acute myocardial infarction, ischemic stroke, and deep vein thrombosis. it has the benefit of generating no adverse effects such as fibrinogen depletion, systemic hemorrhage, and immunologic reactions. human t-pa is a serine-protease enzyme containing 527 amino acid residues in five structural domains. the correct folding of t-pa requires the correct pairing of 17 disulfide bridges in the molecule. a gene encoding ... | 2010 | 20455033 |
| the evolutionary landscape of the chromatin modification machinery reveals lineage specific gains, expansions, and losses. | model organisms such as yeast, fly, and worm have played a defining role in the study of many biological systems. a significant challenge remains in translating this information to humans. of critical importance is the ability to differentiate those components where knowledge of function and interactions may be reliably inferred from those that represent lineage-specific innovations. to address this challenge, we use chromatin modification (cm) as a model system for exploring the evolutionary pr ... | 2010 | 20455264 |
| mds3 regulates morphogenesis in candida albicans through the tor pathway. | the success of candida albicans as a major human fungal pathogen is dependent on its ability to colonize and survive as a commensal on diverse mucosal surfaces. one trait required for survival and virulence in the host is the morphogenetic yeast-to-hypha transition. mds3 was identified as a regulator of ph-dependent morphogenesis that functions in parallel with the classic rim101 ph-sensing pathway. microarray analyses revealed that mds3 delta/delta cells had an expression profile indicative of ... | 2010 | 20457806 |
| substrate specificity of the tim22 mitochondrial import pathway revealed with small molecule inhibitor of protein translocation. | the tim22 protein import pathway mediates the import of membrane proteins into the mitochondrial inner membrane and consists of two intermembrane space chaperone complexes, the tim9-tim10 and tim8-tim13 complexes. to facilitate mechanistic studies, we developed a chemical-genetic approach to identify small molecule agonists that caused lethality to a tim10-1 yeast mutant at the permissive temperature. one molecule, mitoblock-1, attenuated the import of the carrier proteins including the adp/atp ... | 2010 | 20457929 |
| engineering of a human kringle domain into agonistic and antagonistic binding proteins functioning in vitro and in vivo. | here, we report the development of target-specific binding proteins based on the kringle domain (kd) ( approximately 80 residues), a ubiquitous modular structural unit occurring across eukaryotic species. by exploiting the highly conserved backbone folding by core residues, but using extensive sequence variations in the seven loop regions of naturally occurring human kds, we generated a synthetic kd library on the yeast cell surface by randomizing 45 residues in the loops of a human kd template. ... | 2010 | 20460308 |
| application of simple fed-batch technique to high-level secretory production of insulin precursor using pichia pastoris with subsequent purification and conversion to human insulin. | the prevalence of diabetes is predicted to rise significantly in the coming decades. a recent analysis projects that by the year 2030 there will be ~366 million diabetics around the world, leading to an increased demand for inexpensive insulin to make this life-saving drug also affordable for resource poor countries. | 2010 | 20462406 |
| ash2l interacts with tbx1 and is required during early embryogenesis. | tbx1 encodes a dna binding transcription factor that is commonly deleted in human digeorge syndrome and plays an important role in heart development. mechanisms of tbx1 function, such as tbx1 interacting regulatory proteins and transcriptional target specificity, are largely unknown. ash2l is the mammalian homolog of drosophila ash2 (absent small homeotic 2) and is a core component of a multimeric histone methyltransferase complex that epigenetically regulates transcription via methylation of hi ... | 2010 | 20463296 |
| blastomycosis: case report of an isolated lesion in the distal fibula. | blastomycosis (blastomyces dermatitidis) is a fungal infection that occurs primarily in the lungs, but 15% to 60% of patients with systemic blastomycosis have skeletal involvement. because the symptoms and radiographic appearance of bony lesions are variable, diagnosis and treatment may be delayed if fungal infections are not included in the differential diagnosis for a patient with a lytic bone lesion. we present the case of a man in his late 30s with no local or systemic signs of infection in ... | 2010 | 20463990 |
| prediction of functionally selective allosteric interactions at an m3 muscarinic acetylcholine receptor mutant using saccharomyces cerevisiae. | saccharomyces cerevisiae is a tractable yeast species for expression and coupling of heterologous g protein-coupled receptors with the endogenous pheromone response pathway. although this platform has been used for ligand screening, no studies have probed its ability to predict novel pharmacology and functional selectivity of allosteric ligands. as a proof of concept, we expressed a rat m(3) muscarinic acetylcholine receptor (machr) bearing a mutation (k(7.32)e) recently identified to confer pos ... | 2010 | 20466821 |
| a nuclear targeting system in plasmodium falciparum. | the distinct differences in gene control mechanisms acting in the nucleus between plasmodium falciparum and the human host could lead to new potential drug targets for anti-malarial development. new molecular toolkits are required for dissecting molecular machineries in the p. falciparum nucleus. one valuable tool commonly used in model organisms is protein targeting to specific sub-cellular locations. targeting proteins to specified locations allows labeling of organelles for microscopy, or tes ... | 2010 | 20470378 |
| antifungal activity, experimental infections and nail permeation of an innovative ciclopirox nail lacquer based on a water-soluble biopolymer. | p-3051 is an innovative 8% ciclopirox nail lacquer, based on hydroxypropyl chitosan (hpch) as a film-forming agent. the authors' aim was to investigate p-3051's in vitro antifungal activity, as well as its in vitro and in vivo nail permeation. the dilution susceptibility tests performed for trichophyton rubrum (t. rubrum) and candida parapsilosis (c. parapsilosis) showed that the minimum inhibitory concentrations (mics) of p-3051, as percent of ciclopirox, was for both fungi < or = 0.0015% (equi ... | 2010 | 20480796 |
| transcription factors mat2 and znf2 operate cellular circuits orchestrating opposite- and same-sex mating in cryptococcus neoformans. | cryptococcus neoformans is a human fungal pathogen that undergoes a dimorphic transition from a unicellular yeast to multicellular hyphae during opposite sex (mating) and unisexual reproduction (same-sex mating). opposite- and same-sex mating are induced by similar environmental conditions and involve many shared components, including the conserved pheromone sensing cpk1 mapk signal transduction cascade that governs the dimorphic switch in c. neoformans. however, the homeodomain cell identity pr ... | 2010 | 20485569 |
| candida dubliniensis screening using the germ tube test in clinical yeast isolates and prevalence of c. dubliniensis in korea. | the aim of this study was to screen for c. dubliniensis using the germ tube test with human pooled serum (hps) in clinical isolates and investigate the prevalence of c. dubliniensis in korea. among 1,854 yeast strains isolated, 1,404 strains of c. albicans (on the basis of positive results of the germ tube test) and 192 germ tube-negative yeast strains were examined. all 1,596 clinical isolates were examined using the germ tube test with hps, the differential temperature, and nacl tolerance test ... | 2010 | 20486193 |
| shugoshin-pp2a counteracts casein-kinase-1-dependent cleavage of rec8 by separase. | during meiosis, the cohesin complexes that maintain sister chromatid cohesion are lost in a stepwise manner. at meiosis i the cohesin subunit rec8 is cleaved only along the chromosome arms; until meiosis ii it is protected at centromeres by the action of shugoshin (sgo1)-protein phosphatase 2a (pp2a). although this regulation hypothetically involves phosphorylation that is antagonized by sgo1-pp2a, the kinase and substrate that are responsible are as yet unknown. using a genetic screen for 'anti ... | 2010 | 20383139 |
| genome-wide analysis of helicase gene family from rice and arabidopsis: a comparison with yeast and human. | helicases are motor proteins which can catalyze the unwinding of stable rna or dna duplex utilizing mainly atp as source of energy. in this study we have identified complete sets of helicases from rice and arabidopsis. the helicase gene family in rice and arabidopsis contains 115 and 113 genes respectively. these helicases were validated based on their annotations and supported with organization of conserved helicase signature motifs. we have also identified homologs of 64 rice rna and dna helic ... | 2010 | 20383562 |
| gtpase activity plays a key role in the pathobiology of lrrk2. | mutations in the leucine-rich repeat kinase 2 (lrrk2) gene are associated with late-onset, autosomal-dominant, familial parkinson's disease (pd) and also contribute to sporadic disease. the lrrk2 gene encodes a large protein with multiple domains, including functional roc gtpase and protein kinase domains. mutations in lrrk2 most likely cause disease through a toxic gain-of-function mechanism. the expression of human lrrk2 variants in cultured primary neurons induces toxicity that is dependent o ... | 2010 | 20386743 |
| an essential dna strand-exchange activity is conserved in the divergent n-termini of blm orthologs. | the gene mutated in bloom's syndrome, blm, encodes a member of the recq family of dna helicases that is needed to suppress genome instability and cancer predisposition. blm is highly conserved and all blm orthologs, including budding yeast sgs1, have a large n-terminus that binds top3-rmi1 but has no known catalytic activity. in this study, we describe a sub-domain of the sgs1 n-terminus that shows in vitro single-strand dna (ssdna) binding, ssdna annealing and strand-exchange (se) activities. t ... | 2010 | 20389284 |
| integrated phosphoproteomics analysis of a signaling network governing nutrient response and peroxisome induction. | phosphorylation of proteins is a key posttranslational modification in cellular signaling, regulating many aspects of cellular responses. we used a quantitative, integrated, phosphoproteomics approach to characterize the cellular responses of the yeast saccharomyces cerevisiae to the fatty acid oleic acid, a molecule with broad human health implications and a potent inducer of peroxisomes. a combination of cryolysis and urea solubilization was used to minimize the opportunity for reorientation o ... | 2010 | 20395639 |
| duality of the carbohydrate-recognition system of pseudomonas aeruginosa-ii lectin (pa-iil). | the study of pseudomonas aeruginosa-ii lectin (pa-iil) complexes with man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. here, the roles of man oligomers and complexes in pa-iil carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. from the results obtained, it is proposed that high density weak -oh conformation as seen in yeast mannan is also an important pa-iil recognition factor. this finding provides ... | 2010 | 20398656 |
| atr3 encodes a diflavin reductase essential for arabidopsis embryo development. | *the arabidopsis genome possesses two confirmed cytochrome p450 reductase (cpr) genes, atr1 and atr2, together with a third putative homologue, atr3, which annotation is questionable. *phylogenetic analysis classified atr3 as a cpr-like protein sharing homologies with the animal cytosolic dual flavin reductases, nr1 and fre-1, distinct from the microsomal cprs, atr1 and atr2. like nr1 and fre-1, atr3 lacks the n-terminal endoplasmic reticulum (er) anchor domain of cprs and is localized in the cy ... | 2010 | 20406405 |
| mida is a putative methyltransferase that is required for mitochondrial complex i function. | dictyostelium and human mida are homologous proteins that belong to a family of proteins of unknown function called duf185. using yeast two-hybrid screening and pull-down experiments, we showed that both proteins interact with the mitochondrial complex i subunit ndufs2. consistent with this, dictyostelium cells lacking mida showed a specific defect in complex i activity, and knockdown of human mida in hek293t cells resulted in reduced levels of assembled complex i. these results indicate a role ... | 2010 | 20406883 |
| concomitant mutations in the p450 oxidoreductase and androgen receptor genes presenting with 46,xy disordered sex development and androgenization at adrenarche. | undervirilization in males, i.e. 46,xy disordered sex development (46,xy dsd), is commonly caused by either lack of androgen action due to mutant androgen receptor (ar) or deficient androgen synthesis, e.g. due to mutations in 17alpha-hydroxylase (cyp17a1). like all other microsomal cytochrome p450 (cyp) enzymes, cyp17a1 requires electron transfer from p450 oxidoreductase (por). | 2010 | 20410220 |
| biological diversity of saccharomyces yeasts of spontaneously fermenting wines in four wine regions: comparative genotypic and phenotypic analysis. | combination of molecular genetic analysis (karyotyping, pcr-rflp of met2, the its1-its2 region and the nts region) and physiological examination (melibiose and mannitol utilization, sugar-, ethanol- and copper tolerance, killer activity, fermentation vigor and production of metabolites) of yeasts isolated from spontaneously fermenting wines in four wine regions revealed very high diversity in the saccharomyces cerevisiae populations. practically each s. cerevisiae isolate showed a unique pattern ... | 2010 | 20413169 |
| (1,3)-beta-glucans activate both dectin-1 and nlrp3 inflammasome in human macrophages. | beta-glucans are naturally occurring polysaccharides that are the major cell wall components of fungi. recognition of beta-glucans is mediated through a membrane-bound pattern recognition receptor called dectin-1, and gene knock-out studies have shown that dectin-1 plays an important role in antifungal immune response in vivo. in this report, we have studied the effect of large particulate (1,3)-beta-glucans, including curdlan, glucan from baker's yeast, paramylon, and zymosan, on inflammatory r ... | 2010 | 20421639 |
| nsc126188, a piperazine alkyl derivative, induces apoptosis via upregulation of rhob in hela cells. | we describe here a piperazine alkyl derivative, nsc126188, which induced apoptosis of hela cells by upregulating rhob expression. nsc126188 caused multi-septation of fission yeast and hypersensitized a rho3 mutant, which implicates the involvement of functional human homolog rhob. the treatment of cells with nsc126188 induced apoptosis and a dramatic increase in rhob expression. in addition, rhob knockdown using sirna rescued cells from apoptosis, indicating a crucial role of rhob in nsc126188-i ... | 2010 | 20432054 |
| the effects of selenium supplementation on the spontaneously occurring fibroid tumors of oviduct, 8-hydroxy-2'-deoxyguanosine levels, and heat shock protein 70 response in japanese quail. | oxidative stress is known to be an important contributing factor in many chronic diseases. spontaneously occurring benign oviduct leiomyomas are common tumors of the japanese quail (coturnix coturnix japonica), which makes it a good animal model for screening potential agents for testing in the prevention and treatment of human myoma uteri. since dietary intake of selenium has been associated with a reduced risk of a variety of human cancers, we investigated the effects of selenium supplementati ... | 2010 | 20432170 |
| towards universal systems for recombinant gene expression. | recombinant gene expression is among the most important techniques used both in molecular and medical research and in industrial settings. today, two recombinant expression systems are particularly well represented in the literature reporting on recombinant expression of specific genes. according to searches in the pubmed citation database, during the last 15 years 80% of all recombinant genes reported on in the literature were expressed in either the enterobacterium escherichia coli or the meth ... | 2010 | 20433754 |
| from protein interaction profile to functional assignment: the human protein ki-1/57 is associated with pre-mrna splicing events. | the mapping of protein-protein interactions of a determined organism is considered fundamental to assign protein function in the post-genomic era. as part of this effort, screenings for pairwise interactions by yeast two-hybrid system have been used popularly to reveal protein interaction networks in different biological systems. through the identification of protein interaction partners we have successfully obtained interesting functional clues for ki-1/57, a human protein with no previous func ... | 2010 | 20436279 |
| htlv-1 basic leucine-zipper factor, hbz, interacts with mafb and suppresses transcription through a maf recognition element. | htlv-1 infection causes adult t-cell leukemia (atl). the development of atl is thought to be associated with disruption of transcriptional control of cellular genes. htlv-1 basic leucine-zipper (bzip) factor, hbz, is encoded by the complementary strand of the provirus. we previously reported that hbz interacts with c-jun and suppresses its transcriptional activity. to identify the cellular factor(s) that interact with hbz, we conducted a yeast two-hybrid screen using full-length hbz as bait and ... | 2010 | 20506502 |
| identification of cysteine-rich epidermal growth factor-like domain 1alpha (creld1alpha) as a novel alpha1a-adrenoceptor-down-regulating protein and establishment of an alpha1l-adrenoceptor-expressing cell line. | two distinct alpha(1)-adrenoceptor phenotypes (alpha(1a)- and alpha(1l)-ars) are known to originate from a single adra1a(alpha(1a)) gene by an as-yet-unknown mechanism. we hypothesized that an alpha(1a)-ar-interacting protein could generate the alpha(1l)-ar phenotype and we sought to identify such a protein and to examine its effects on the expression of alpha(1a) and alpha(1l) phenotypes. cysteine-rich epidermal growth factor-like domain 1alpha (creld1alpha) was first identified using a yeast t ... | 2010 | 20508391 |
| duplicability of self-interacting human genes. | there is increasing interest in the evolution of protein-protein interactions because this should ultimately be informative of the patterns of evolution of new protein functions within the cell. one model proposes that the evolution of new protein-protein interactions and protein complexes proceeds through the duplication of self-interacting genes. this model is supported by data from yeast. we examined the relationship between gene duplication and self-interaction in the human genome. | 2010 | 20509897 |
| struct2net: a web service to predict protein-protein interactions using a structure-based approach. | struct2net is a web server for predicting interactions between arbitrary protein pairs using a structure-based approach. prediction of protein-protein interactions (ppis) is a central area of interest and successful prediction would provide leads for experiments and drug design; however, the experimental coverage of the ppi interactome remains inadequate. we believe that struct2net is the first community-wide resource to provide structure-based ppi predictions that go beyond homology modeling. a ... | 2010 | 20513650 |