Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
metal composition and solubility determine lung toxicity induced by residual oil fly ash collected from different sites within a power plant.residual oil fly ash (rofa) is a particulate pollutant comprised of soluble and insoluble metals and is produced by the combustion of fossil fuels. the objective was to examine the pulmonary responses to chemically distinct rofa samples collected from either a precipitator or air heater within the same power plant. the collected rofa samples were suspended in saline (total sample), incubated for 24 h at 37 degrees c, centrifuged, separated into soluble and insoluble fractions, and the metal comp ...200414971666
[listeriosis in the adult. revision of 10 cases].listeria monocytogenes is still a very rare opportunist infection in immunosuppressive patients. the clinical-epidemiological and therapeutic characteristics in 10 patients with infection produced by lm are reported--four of them had primary bacteriemia, three patients had a meningeal involvement, there were two patients with spontaneous bacterial peritonitis and one suffered from abdominal access. all of the patients had underlying disorders favouring the infection. sepsis and meningeal syndrom ...200414974893
die-off of enteric bacterial pathogens during mesophilic anaerobic digestion.conventionally treated sewage sludge may contain high concentrations of potentially pathogenic microorganisms and additional treatment is required to minimise the risks to health if it is to be recycled to agricultural land. mesophilic anaerobic digestion (mad) is the most widely used process in the uk for stabilising sludge prior to agricultural recycling, but little is known about the fate of a number of enteric pathogens as the sludge passes through the treatment processes. the aim of this st ...200414975643
intact type 1 immunity and immune-associated coagulative responses in mice lacking ifn gamma-inducible fibrinogen-like protein 2.fibrinogen-like protein 2 (fgl2, fibroleukin) is a leukocyte product that exhibits significant homology to secreted proteins of diverse function, including growth factors, lectins, and components of extracellular matrix. prior studies found that fgl2 is ifn gamma-inducible, possesses direct coagulant activity, and inhibits t cell proliferation and dendritic cell maturation in vitro. here, we demonstrate that fgl2 expression is up-regulated during type 1 immunity in vivo and establish that such u ...200414976252
a ratiometric expressible fret sensor for phosphoinositides displays a signal change in highly dynamic membrane structures in fibroblasts.phosphoinositides are important signal transduction intermediates in cell growth, survival, and motility. we have invented a fluorescence sensor for polyphosphorylated phosphoinositides based on a peptide derived from the listeria protein acta that undergoes a random coil to helix transition upon lipid binding. the sensor, termed cay, is a fusion protein of cyan and yellow fluorescent proteins flanking the peptide at its n- and c-termini, respectively. cay displays fluorescence resonance energy ...200414967034
detection of pcr amplicons from bacterial pathogens using microsphere agglutination.for rapid and inexpensive detection of polymerase chain reaction (pcr) amplicons, a novel microsphere agglutination assay has been developed. pcr is carried out using biotinylated forward and reverse primers, and the amplified dna fragments are able to agglutinate streptavidin-coated microspheres (5.7 microm in diameter). purification of pcr amplicons is unnecessary when initial primer concentrations are 250 nm. agglutination can be identified visually within 2 min without any additional equipme ...200414967231
recontamination as a source of pathogens in processed foods.food products that have been submitted to an adequate heat-treatment during processing are free of vegetative pathogens and, depending on the treatments, of sporeformers and are generally regarded as safe. processed products such as pâté, ice cream, infant formulae and others have nevertheless been responsible for food-borne illnesses. thorough epidemiological investigations of several of these outbreaks have demonstrated that the presence of vegetative pathogens such as salmonella spp. or liste ...200414967555
effects of chlorine and ph on efficacy of electrolyzed water for inactivating escherichia coli o157:h7 and listeria monocytogenes.the effects of chlorine and ph on the bactericidal activity of electrolyzed (eo) water were examined against escherichia coli o157:h7 and listeria monocytogenes. the residual chlorine concentration of eo water ranged from 0.1 to 5.0 mg/l, and the ph effect was examined at ph 3.0, 5.0, and 7.0. the bactericidal activity of eo water increased with residual chlorine concentration for both pathogens, and complete inactivation was achieved at residual chlorine levels equal to or higher than 1.0 mg/l. ...200414967556
influence of the sigb gene on the cold stress survival and subsequent recovery of two listeria monocytogenes serotypes.the influence of serotype and the role of the sigb gene of listeria monocytogenes during the survival and recovery on different substrates were determined. wild-type and sigb mutants of two serotypes of l. monocytogenes were inoculated into buffer and onto beef steaks, and incubated at 4 degrees c for 8 weeks during which samples were removed and listeria numbers determined. growth kinetics of stationary phase wild-type and sigb mutant cells were compared, without prechilling and after prechilli ...200414967561
inhibition of foodborne bacteria by the lactoperoxidase system in a beef cube system.biopreservatives are being developed to inhibit the growth of foodborne pathogens and thus improve food safety. the lactoperoxidase system (lps) is a naturally occurring system that has potential for use as an antimicrobial agent in foods. growth of single strains of the pathogens staphylococcus aureus, listeria monocytogenes, escherichia coli o157:h7, salmonella enterica subsp. enterica serovar typhimurium, yersinia enterocolitica, pseudomonas aeruginosa and beef microflora were assessed on lps ...200414967562
viability loss and morphology change of foodborne pathogens following exposure to hydrostatic pressures in the presence and absence of bacteriocins.cell suspensions of three pathogens were exposed to hydrostatic pressure (hp), bacteriocin mixture (nisin and pediocin) or a combination of hp+bacteriocins and changes in colony forming units (cfu) and cell-morphology by scanning electron microscopy (sem) were studied. cell viability loss, as determined from the reduction in cfu before and after a treatment, occurred in listeria monocytogenes by all three treatments and in salmonella typhimurium and escherichia coli o157:h7 by hp and hp+bacterio ...200414967564
prevalence of escherichia coli o157:h7, listeria monocytogenes, and salmonella in two geographically distant commercial beef processing plants in the united states.for two large beef processing plants, one located in the southern united states (plant a) and one located in the northern united states (plant b), prevalence of escherichia coli o157:h7, listeria spp., listeria monocytogenes, and salmonella was determined for hide, carcass, and facility environmental samples over the course of 5 months. the prevalence of e. coli o157:h7 (68.1 versus 55.9%) and salmonella (91.8 versus 50.3%) was higher (p < 0.05), and the prevalence of listeria spp. (37.7 versus ...200414968961
effects of cetylpyridinium chloride, acidified sodium chlorite, and potassium sorbate on populations of escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus on fresh beef.the effects of selected food-grade antimicrobial agents at decreasing the number of pathogenic bacteria on fresh beef were determined. beef cubes inoculated with escherichia coli o157:h7, listeria monocytogenes, or staphylococcus aureus were sprayed with 0.5% cetylpyridinium chloride (cpc), 0.12% acidified sodium chlorite (asc), 0.1% potassium sorbate (ps), or an equal mix of any two solutions. the beef samples were placed on absorbent tray pads sprayed with each single or mixed solution, wrappe ...200414968963
changes in heat resistance resulting from ph and nutritional shifts of acid-adapted and non-acid-adapted listeria monocytogenes scott a.stationary-phase listeria monocytogenes cells that were either ph dependent acid adapted or not acid adapted were heat challenged at 60 degrees c in a two-level full factorial design for three variables. the three variables and the levels consisted of tryptic soy broth (tsb) and sterile cell-free culture supernatant (sterile tsb), the presence and absence of 1% added glucose, and ph 4.8 and ph 7. non-acid-adapted cells were most heat resistant when challenged in tsb (mean decimal reduction times ...200414968964
behavior of listeria monocytogenes in a pseudomonas putida biofilm on a condensate-forming surface.listeria monocytogenes has been isolated from condensate-forming surfaces in food processing plants. the objective of this research was to observe the behavior of l. monocytogenes on condensate-covered stainless steel with a pseudomonas putida biofilm. l. monocytogenes-containing biofilms, either with or without added chicken protein, were incubated in a high humidity chamber at 12 degrees c to allow formation of condensate. samples were analyzed for attached and unattached l. monocytogenes and ...200414968965
tracking of listeria monocytogenes in smoked fish processing plants.four smoked fish processing plants were used as a model system to characterize listeria monocytogenes contamination patterns in ready-to-eat food production environments. each of the four plants was sampled monthly for approximately 1 year. at each sampling, four to six raw fish and four to six finished product samples were collected from corresponding lots. in addition, 12 to 14 environmental sponge samples were collected several hours after the start of production at sites selected as being li ...200414968966
control of bacterial pathogens during processing of cold-smoked and dried salmon strips.microbiological and chemical changes were determined during the smoking and drying of salmon strips processed at 29 to 31 degrees c for 4 days at a facility in alaska in 1993. during the process, staphylococcus aureus populations increased to more than 10(5) cfu/g after 2 to 3 days of processing. subsequent laboratory studies showed that a pellicle (dried skinlike surface) formed rapidly on the strips when there was rapid air circulation in the smokehouse and that bacteria embedded in or under t ...200414968968
survival of listeria monocytogenes in vanilla-flavored soy and dairy products stored at 8 degrees c.the survival of listeria monocytogenes v37 in vanilla-flavored yogurt (low-fat and nonfat) and soy milk (low-fat and plus) stored at 8 degrees c for 31 days was investigated. commercial samples of yogurt and soy milk were used. these samples were inoculated with either 10(4) or 10(7) cfu of l. monocytogenes per ml. sampling was carried out every 3 to 4 days initially and was then carried out weekly, for a total storage time of 31 days. each time a sample was collected, the ph of the sample was m ...200414968973
delayed presentation of prosthetic joint infection due to listeria monocytogenes.infection with listeria monocytogenes is rare and has been described in prosthetic valves, stent grafts and prosthetic joints. the route of infection appears to be haematogenous. the choice between conservative treatment with antibiotics or surgical treatment with debridement and revision of the components remains controversial. the best antibiotic treatment is not known with ampicillin being the first choice in most cases. prosthetic infections with listeria monocytogenes usually occur in patie ...200415161131
investigation of the effectiveness of ascopyrone p as a food preservative.ascopyrone p (app), a novel antibacterial from fungi, was evaluated as a food preservative. efficacy was generally assessed by comparing the time taken for test strains to grow to 10(6) cfu/g in food +/- app. in chilled chicken soup, 2000 mg kg-1 app prevented bacillus cereus, listeria monocytogenes, pseudomonas fluorescens, salmonella and escherichia coli reaching this threshold for >60 days. good activity was also observed at 500-1000 mg kg-1 but not against l. monocytogenes. no activity was o ...200415163588
evaluation of the bax system for detection of listeria monocytogenes in foods: collaborative study.a multilaboratory study was conducted to compare the automated bax system and the standard cultural methods for detection of listeria monocytogenes in foods. six food types (frankfurters, soft cheese, smoked salmon, raw, ground beef, fresh radishes, and frozen peas) were analyzed by each method. for each food type, 3 inoculation levels were tested: high (average of 2 cfu/g), low (average of 0.2 cfu/g) and uninoculated controls. a total of 25 laboratories representing government and industry part ...200415164834
comparison of selective and nonselective primary enrichments for the detection of listeria monocytogenes in cheese.a completely selective enrichment procedure was compared with two partially nonselective ones for the detection of listeria monocytogenes in cheeses. after enrichment for approximately 48 h, the enrichment media were streaked on selective agars and presumptive listeria colonies were confirmed using pcr. in some cases, pcr was also performed directly on the enrichment broth. the conventional, completely selective enrichment procedure was not always the best choice for the detection of stressed l. ...200415172481
a process for controlling intracellular bacterial infections induced by membrane injury.strategies for inhibiting phagolysosome fusion are essential for the intracellular survival and replication of many pathogens. we found that the lysosomal synaptotagmin syt vii is required for a mechanism that promotes phagolysosomal fusion and limits the intracellular growth of pathogenic bacteria. syt vii was required for a form of ca2+-dependent phagolysosome fusion that is analogous to ca2+-regulated exocytosis of lysosomes, which can be triggered by membrane injury. bacterial type iii secre ...200415178804
linearized kinetic model of listeria monocytogenes biofilm growth.in this study the dynamics of biofilm formation on aluminum has been investigated. the process of cell growth has been observed using fluorescence microscopy. it has been confirmed that the process of biofilm formation can be represented as a sum of two separate processes: cell adhesion and colony proliferation. the derived set of equations describes kinetics of surface population growth and characteristic times for adsorption and combined growth processes, including characteristic time for the ...200415179574
novel expression system for large-scale production and purification of recombinant class iia bacteriocins and its application to piscicolin 126.piscicolin 126 is a class iia bacteriocin isolated from carnobacterium piscicola jg126 that exhibits strong activity against listeria monocytogenes. the gene encoding mature piscicolin 126 (m-pisa) was cloned into an escherichia coli expression system and expressed as a thioredoxin-piscicolin 126 fusion protein that was purified by affinity chromatography. purified recombinant piscicolin 126 was obtained after cnbr cleavage of the fusion protein followed by reversed-phase chromatography. recombi ...200415184123
identification of sigma factor sigma b-controlled genes and their impact on acid stress, high hydrostatic pressure, and freeze survival in listeria monocytogenes egd-e.the gene encoding the alternative sigma factor sigma(b) in listeria monocytogenes is induced upon exposure of cells to several stresses. in this study, we investigated the impact of a sigb null mutation on the survival of l. monocytogenes egd-e at low ph, during high-hydrostatic-pressure treatment, and during freezing. the survival of delta sigb mutant exponential-phase cells at ph 2.5 was 10,000-fold lower than the survival of egd-e wild-type cells. moreover, the delta sigb mutant failed to sho ...200415184144
modeling the rate of attachment of listeria monocytogenes, pantoea agglomerans, and pseudomonas fluorescens to, and the probability of their detachment from, potato tissue at 10 degrees c.the rate of attachment of bacteria to, and their subsequent detachment from, the cut surface of raw potato tissue was measured and modeled by using mathematical approaches that allowed detailed objective comparisons of adhesion processes under different conditions. attachment was rapid and reached equilibrium after contact for 60 min. a new method to measure the probability of detachment was developed and modeled, revealing that the probability of detachment for pseudomonas fluorescens remained ...200415184157
identification and characterization of the novel lysm domain-containing surface protein sep from lactobacillus fermentum br11 and its use as a peptide fusion partner in lactobacillus and lactococcus.examination of supernatant fractions from broth cultures of lactobacillus fermentum br11 revealed the presence of a number of proteins, including a 27-kda protein termed sep. the amino-terminal sequence of sep was determined, and the gene encoding it was cloned and sequenced. sep is a 205-amino-acid protein and contains a 30-amino-acid secretion signal and has overall homology (between 39 and 92% identity) with similarly sized proteins of lactobacillus reuteri, enterococcus faecium, streptococcu ...200415184172
new listeria monocytogenes prfa* mutants, transcriptional properties of prfa* proteins and structure-function of the virulence regulator prfa.prfa, a transcription factor structurally related to crp/fnr, activates listeria monocytogenes virulence genes during intracellular infection. we report two new prfa* mutations causing the constitutive overexpression of the prfa regulon. leu-140phe lies in alphad adjacent to the dna-binding motif in the c-terminal domain, like a previously characterized prfa* mutation (gly-145ser). ile-45ser, in contrast, maps to the n-terminal beta-roll, a structure similar to that of the crp camp binding site. ...200415186408
the autolytic phenotype of bacillus thuringiensis.to evaluate the autolytic phenotype of bacillus thuringiensis.200415186453
development of tcr alpha beta cd8 alpha alpha intestinal intraepithelial lymphocytes is promoted by interleukin-15-producing epithelial cells constitutively stimulated by gram-negative bacteria via tlr4.the microbes present in the intestine have a strong influence on the development and maturation of lymphoid organs. the cross-talk mechanisms between intestinal intraepithelial lymphocytes (i-iel) and noninvasive microbes are still poorly understood. the influence of microbes and lipopolysaccharides on the development of i-iel, especially the tcr alpha beta(+) cd8 alpha alpha subset, was investigated using the different tlr4-mutant mouse strains c3h/hej, balb/lps(d), and c57bl/10sccr. intestinal ...200415187438
[influence of temperature on the variability of listeria monocytogenes in the course of its prolonged existence in soil-packed water-circulating columns].the capacity of l. monocytogenes for prolonged existence in the soil at different temperatures (18-20 degrees c and 6-8 degrees c) has been shown. the viability of these bacteria in the soil depends both on their properties and the temperature factor. low temperatures (6-8 degrees c) ensure, on the whole, the stable preservation of the biological properties of the bacteria. more pronounced variability of l. monocytogenes at a temperature of 18-20 degrees c is an adaptive property, ensuring high ...200415188555
inhibition of tnf-alpha production in thp-1 macrophages by glatiramer acetate does not alter their susceptibility to infection by listeria monocytogenes and does not impair the efficacy of ampicillin or moxifloxacin against intracellular bacteria. 200415190038
diversity of listeria monocytogenes isolates of human and food origin studied by serotyping, automated ribotyping and pulsed-field gel electrophoresis.automated ribotyping, pulsed-field gel electrophoresis (pfge) and serotyping were evaluated for the epidemiological study of isolates of listeria monocytogenes collected in finland in 1997-1999 from human blood (n = 116) and the food industry (n = 72). the isolates divided into six serotypes, 23 ecori ribotypes, 54 asci pfge types, and 57 final subtypes if all results were combined. the discrimination index of ribotyping was lower (0.873) than that of pfge (0.946). two final subtypes dominated a ...200415191386
interactions between high pressure homogenization and antimicrobial activity of lysozyme and lactoperoxidase.it was the objective of this work to evaluate the effect of high pressure homogenization on the activity of antimicrobial enzymes such as lysozyme and lactoperoxidase against a selected group of gram positive and gram negative species inoculated in skim milk. lactobacillus helveticus, lactobacillus plantarum and listeria monocytogenes were the most pressure resistant species while bacillus subtilis, pseudomonas putida, salmonella typhimurium, staphylococcus aureus, proteus vulgaris and salmonell ...200415193800
recombinant escherichia coli as a gene delivery vector into airway epithelial cells.to transfer genes into airway epithelial cells, we have generated auxotrophic dap escherichia coli bm2710 mutant that expresses the invasin of yersinia pseudotuberculosis and the listeriolysin of listeria monocytogenes. e. coli bm2710 harboring a plasmid carrying the gfp gene was incubated with immortalized normal or cystic fibrosis (cf) airway epithelial cells or with primary bronchial epithelial cells grown as an explant-outgrowth cell culture model. approximately 2% of immortalized cells expr ...200415196763
the role of substrate curvature in actin-based pushing forces.the extension of the plasma membrane during cell crawling or spreading is known to require actin polymerization; however, the question of how pushing forces derive from actin polymerization remains open. a leading theory (herein referred to as elastic propulsion) illustrates how elastic stresses in networks growing on curved surfaces can result in forces that push particles. to date all examples of reconstituted motility have used curved surfaces, raising the possibility that such squeezing forc ...200415203003
actin polymerization: forcing flat faces forward.actin polymerization has been shown to be sufficient to propel curved objects, for example beads and vesicles coated with the listeria monocytogenes protein acta. recent studies suggest that actin polymerization on flat surfaces can also provide the propulsive force to push them forward.200415203017
brainstem and cranial nerve abnormalities: listeriosis, otitis media/interna, and pituitary abscess syndrome.this article reviews three disorders associated with multiple asymmetric cranial nerve deficits in ruminants: encephalitic listeriosis,otitis media/interna, and pituitary abscess syndrome. emphasis is placed on encephalitic listeriosis, an infectious disease of the brainstem and cranial nerves caused by listeria monocytogenes. the epidemiology, pathophysiology, clinical manifestations, diagnosis,and treatment of encephalitic listeriosis are reviewed, and differences between cattle and small rumi ...200415203225
listeria monocytogenes pneumonia in a cirrhotic child.a 13-year-old girl with cirrhosis and cyanotic heart disease was admitted with a three-day history of pneumonia. the chest roentgenogram revealed left-sided pleural effusion and cultures from the pleural fluid yielded listeria monocytogenes. the authors discuss the epidemiologic, clinical, and pathophysiological aspects of l. monocytogenes pneumonia and its association with cirrhosis.200415206516
effects of applying safe2o poultry wash to broiler wings on shelf life, listeria monocytogenes, pseudomonads, staphylococcus species, and psychrotrophic bacteria levels after three, seven, and ten days of storage.bacterial contamination of raw processed poultry continues to be of concern to consumers as well as regulatory and health officials. for many years wings were considered a low-value product; therefore, shelf life of wings was not a major concern. due to changes in consumer attitudes and increases in the fast-food market, wings are now a valuable commodity. because wings have a shorter shelf life than most other raw poultry products, acceptable intervention to decrease the population of associate ...200415206635
a rare case of listeria monocytogenes presenting as prosthetic valve bacterial endocarditis and aortic root abscess.listeria monocytogenes is a gram-positive bacillus that is rarely associated with infections in the general population. those susceptible to this pathogen include neonates, pregnant women, and the immunocompromised. the most common clinical manifestations of listeriosis are bacteremia and meningitis. endocarditis caused by l. monocytogenes is rare with less than 60 cases reported in the world literature. we report the case of an 81-year-old man who suffered aortic prosthetic valve listeria endoc ...200415209721
listeria monocytogenes as a vaccine vector: virulence attenuation or existing antivector immunity does not diminish therapeutic efficacy.the bacterium l. monocytogenes is a proposed vaccine carrier based upon the observation that this pathogen replicates within the intracytoplasmic environment facilitating delivery of ag to the endogenous ag processing and presentation pathway with subsequent stimulation of peptide specific mhc class i-restricted cd8(+) effector cells. in this report, we evaluate virulence-attenuated strains of listeria monocytogenes as vaccine vectors and examine whether existing antivector (antilisterial) immun ...200415210801
intestinal p glycoprotein acts as a natural defense mechanism against listeria monocytogenes.mechanisms by which the intestinal epithelium resists invasion by food-borne pathogens such as listeria monocytogenes are an evolving area of research. intestinal p glycoprotein is well known to limit the absorption of xenobiotics and is believed to act as a cytotoxic defense mechanism. the aim of this study was to determine if intestinal p glycoprotein is involved in host defense against l. monocytogenes. caco-2 cells and a p-glycoprotein-overexpressing subclone (caco-2/mdr) were employed in ad ...200415213126
oral inoculation of a/j mice for detection of invasiveness differences between listeria monocytogenes epidemic and environmental strains.four-week-old harlan a/j mice were orally infected with six epidemic and six environmental strains of listeria monocytogenes. epidemic strains were significantly more invasive as a group than were environmental strains (p < 0.05), and the intestines of some mice infected with epidemic strains had extensive hemorrhage. mice inoculated with epidemic strains were significantly more likely to become systemically infected than mice inoculated with environmental strains (p < 0.01).200415213183
canine tonsillitis associated with listeria monocytogenes. 200415214521
bacteriocin-like substance production by bacillus licheniformis strain p40.to investigate the production of bacteriocin-like compounds by bacillus spp. isolated from the amazon basin.200415214721
comparative acid stress response of listeria monocytogenes, escherichia coli o157:h7 and salmonella typhimurium after habituation at different ph conditions.the aim of the study was to evaluate the effect of habituation at different ph conditions on the acid resistance of listeria monocytogenes, escherichia coli o157:h7 and salmonella enterica serotype typhimurium, and to identify potential differences between the adaptive responses of the three pathogens.200415214733
survival and recovery of escherichia coli o157:h7, salmonella, and listeria monocytogenes on lettuce and parsley as affected by method of inoculation, time between inoculation and analysis, and treatment with chlorinated water.the effects of method for applying inoculum and of drying time after inoculation on survival and recovery of foodborne pathogens on iceberg lettuce and parsley were studied. five-strain mixtures of escherichia coli o157:h7, salmonella, or listeria monocytogenes were applied to lettuce and parsley by dip, spot, or spray inoculation methods. inocula were dried for 2 h at 22 degrees c or for 2 h at 22 degrees c and then 22 h at 4 degrees c before being treated with water (control) or chlorine (200 ...200415222533
impact of intervention strategies on listeria contamination patterns in crawfish processing plants: a longitudinal study.two ready-to-eat crawfish processing plants were monitored for 2 years to study the impact of listeria control strategies, including employee training and targeted sanitation procedures, on listeria contamination. environmental, raw material, and finished product samples were collected weekly during the main processing months (april to june) and tested for listeria spp. and listeria monocytogenes. before implementation of control strategies (year 1), the two processing plants showed listeria spp ...200415222544
survival of listeria monocytogenes strain h7762 and resistance to simulated gastric fluid following exposure to frankfurter exudate.listeria monocytogenes strain h7762, a frankfurter isolate, was tested to determine whether it was able to survive at 4 degrees c in frankfurter pack fluid (exudate) and to determine whether food exposure affects its acid sensitivity. cultures were sampled and tested for acid sensitivity by challenge with simulated gastric fluid (sgf). sgf challenges performed immediately after inoculation revealed that between 20 and 26% of the cells survived the full 30 min of sgf challenge regardless of wheth ...200415222545
efficacy of chlorine and a peroxyacetic acid sanitizer in killing listeria monocytogenes on iceberg and romaine lettuce using simulated commercial processing conditions.the efficacy of chlorine (100 microg/ml) and a peroxyacetic acid sanitizer (80 microg/ml; tsunami 100) in killing listeria monocytogenes inoculated at populations of 1 to 2, 2 to 3, and 4 to 5 log cfu/g of iceberg lettuce pieces, shredded iceberg lettuce, and romaine lettuce pieces was determined by treatment conditions simulating those used by a commercial fresh-cut lettuce processor. the lettuce/treatment solution ratio was 1:100 (wt/vol), treatment temperature was 4 degrees c, and total treat ...200415222557
in vitro antimicrobial activity of essential oils from aromatic plants against selected foodborne pathogens.the purpose of this study was to examine the effectiveness of selected essential oils for the control of growth and survival of pathogenic microorganisms of significant importance in food hygiene and to determine whether the antimicrobial effect was due to the major compounds of the oils. mic and mbc were determined by the tube dilution method. essential oils from thymus vulgaris from spain and france, salvia sclarea, salvia officinalis, salvia lavandulifolia, lavandula latifolia, lavandula angu ...200415222560
fbpa, a novel multifunctional listeria monocytogenes virulence factor.listeria monocytogenes is a gram-positive intracellular bacterium responsible for severe opportunistic infections in humans and animals. signature-tagged mutagenesis (stm) was used to identify a gene named fbpa, required for efficient liver colonization of mice inoculated intravenously. fbpa was also shown to be required for intestinal and liver colonization after oral infection of transgenic mice expressing human e-cadherin. fbpa encodes a 570-amino-acid polypeptide that has strong homologies t ...200415228540
clinical and histopathological aspects of naturally occurring mastitis caused by listeria monocytogenes in cattle and ewes.listeria monocytogenes was isolated from the milk of two cows and two sheep with mastitis in one quarter and one udder half. the animals were observed over a period of 2-12 months. clinical examination of the udder, bacteriological examinations and determination of somatic cell counts of milk samples were performed monthly. all four cases suffered from a subclinical mastitis characterized by an elevated somatic cell count (0.8-10.1 x 10(6) cells/ml), a persistent shedding of listeria and by a no ...200415228552
a rickettsia wasp-like protein activates the arp2/3 complex and mediates actin-based motility.spotted fever group rickettsia are obligate intracellular pathogens that exploit the host cell actin cytoskeleton to promote motility and cell-to-cell spread. although other pathogens such as listeria monocytogenes use an arp2/3 complex-dependent nucleation mechanism to generate comet tails consisting of y-branched filament arrays, rickettsia polymerize tails consisting of unbranched filaments by a previously unknown mechanism. we identified genes in several rickettsia species encoding proteins ...200415236643
development of polythene films for food packaging activated with an antilisterial bacteriocin from lactobacillus curvatus 32y.the aims of this work were to (i) use a bacteriocin produced by lactobacillus curvatus 32y active against listeria monocytogenes to activate polythene films by different methods, (ii) implement a large-scale process for antilisterial polythene films production and (iii) verify the efficacy of the developed films in inhibiting the growth of l. monocytogenes during the storage of meat products.200415239697
new chromogenic plating media for detection and enumeration of pathogenic listeria spp.--an overview.in recent years a number of selective chromogenic plating media for pathogenic listeria spp. have been developed and marketed. their advantages are direct detection and enumeration of pathogenic listeria spp. utilizing cleavage of substrates by the virulence factor phosphatidylinositol-phospholipase c (pi-plc) and, to a lesser extent, by phosphatidylcholin-phospholipase c (pc-plc). there are two groups of such media: the first utilizes cleavage by pi-plc of l-alpha-phosphatidyl-inositol, forming ...200415240069
control of listeria monocytogenes in a biofilm by competitive-exclusion microorganisms.biofilms from drains in food processing facilities with a recent history of no detectable listeria monocytogenes in floor drains were cultured for microorganisms producing antilisterial metabolites. a total of 413 microbial isolates were obtained from 12 drain biofilm samples and were assayed at 15 and 37 degrees c for activities that were bactericidal or inhibitory to l. monocytogenes, by two agar plate assays. twenty-one of 257 bacterial isolates and 3 of 156 yeast isolates had antilisterial a ...200415240275
co2- and anaerobiosis-induced changes in physiology and gene expression of different listeria monocytogenes strains.although carbon dioxide (co(2)) is known to inhibit growth of most bacteria, very little is known about the cellular response. the food-borne pathogen listeria monocytogenes is characterized by its ability to grow in high co(2) concentrations at refrigeration temperatures. we examined the listerial responses of different strains to growth in air, 100% n(2), and 100% co(2). the co(2)-induced changes in membrane lipid fatty acid composition and expression of selected genes were strain dependent. t ...200415240290
epidemic clone i-specific genetic markers in strains of listeria monocytogenes serotype 4b from foods.listeria monocytogenes contamination of ready-to-eat foods has been implicated in numerous outbreaks of food-borne listeriosis. however, the health hazards posed by l. monocytogenes detected in foods may vary, and speculations exist that strains actually implicated in illness may constitute only a fraction of those that contaminate foods. in this study, examination of 34 serogroup 4 (putative or confirmed serotype 4b) isolates of l. monocytogenes obtained from various foods and food-processing e ...200415240296
natural atypical listeria innocua strains with listeria monocytogenes pathogenicity island 1 genes.identification of bona fide listeria isolates into the six species of the genus normally requires only a few tests. aberrant isolates do occur, but even then only one or two extra confirmatory tests are generally needed for identification to species level. we have discovered a hemolytic-positive, rhamnose and xylose fermentation-negative listeria strain with surprising recalcitrance to identification to the species level due to contradictory results in standard confirmatory tests. the issue had ...200415240309
fully functional memory cd8 t cells in the absence of cd4 t cells.the role of cd4 t cells in providing help to cd8 t cells in primary and secondary responses to infection remains controversial. using recombinant strains of virus and bacteria expressing the same ag, we determined the requirement for cd4 t cells in endogenous cd8 t cell responses to infection with vesicular stomatitis virus and listeria monocytogenes (lm). depletion of cd4 t cells had no effect on the frequency of primary or secondary vesicular stomatitis virus-specific cd8 t cells in either lym ...200415240684
the antimicrobial activity of extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents.the antimicrobial activity of the chloroform, diethyl ether, acetone, petroleum ether, and ethanol extracts of the lichen cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents against 9 bacteria and fungi has been investigated. the extracts and pure compounds alone were found active against the same bacteria and the same yeasts. bacillus cereus, bacillus subtilis, staphylococcus aureus, streptococcus faecalis, proteus vulgaris, listeria monocytogenes, aero ...200415241936
a specific gene expression program triggered by gram-positive bacteria in the cytosol.innate and adaptive immunity depends critically on host recognition of pathogen-associated molecules. toll-like receptors (tlrs) are key mediators of pathogen surveillance at the cell or phagocytic vacuole surface. however, mechanisms underlying recognition of pathogens in other cellular compartments remain unclear, and responses elicited by cytosolic challenge are poorly characterized. we therefore used mouse cdna microarrays to investigate gene expression triggered by infection of bone marrow- ...200415269347
efficacy of chlorine dioxide gas as a sanitizer of lettuce leaves.aqueous solutions of sodium hypochlorite or hypochlorous acid are typically used to sanitize fresh fruits and vegetables. however, pathogenic organisms occasionally survive aqueous sanitization in sufficient numbers to cause disease outbreaks. chlorine dioxide (clo2) gas generated by a dry chemical sachet was tested against foodborne pathogens on lettuce leaves. lettuce leaves were inoculated with cocktail of three strains each of escherichia coli o157:h7, listeria monocytogenes, and salmonella ...200415270488
thermal process validation for escherichia coli o157:h7, salmonella, and listeria monocytogenes in ground turkey and beef products.at 55 to 70 degrees c, thermal inactivation d-values for escherichia coli o157:h7, salmonella, and listeria monocytogenes were 19.05 to 0.038, 43.10 to 0.096, and 33.11 to 0.12 min, respectively, in ground turkey and 21.55 to 0.055, 37.04 to 0.066, and 36.90 to 0.063 min, respectively, in ground beef. the z-values were 5.73, 5.54, and 6.13 degrees c, respectively, in ground turkey and 5.43, 5.74, and 6.01 degrees c, respectively, in ground beef. in both ground turkey and beef, significant (p < 0 ...200415270492
effect of sodium lactate on thermal inactivation of listeria monocytogenes and salmonella in ground chicken thigh and leg meat.the effect of sodium lactate on thermal inactivation d- and z-values of listeria monocytogenes and salmonella was determined for chicken thigh and leg meat. at 55 to 70 degrees c, the d-value of l. monocytogenes in ground chicken thigh and leg meat with the addition of 4.8% sodium lactate (4.8 g sodium lactate per 100 g of meat) was 53 to 75% higher than that in the meat without sodium lactate. no significant difference was found for the d-values of salmonella at 55 to 70 degrees c between the m ...200415270493
use of carvacrol and cymene to control growth and viability of listeria monocytogenes cells and predictions of survivors using frequency distribution functions.the antibacterial action of carvacrol and cymene on two listeria monocytogenes strains (stcc4031 and nctn4032) was studied. carvacrol or cymene showed inhibitory effect on the growth of l. monocytogenes during lag and exponential growth phases and was more evident with increasing concentrations in brain heart infusion broth at 30 degrees c. carvacrol or cymene also decreased the survival of mid-exponential-growth-phase l. monocytogenes stcc4031 cells in potassium-n-2-hydroxy-ethylpiperazine-n-et ...200415270494
distribution of listeria monocytogenes molecular subtypes among human and food isolates from new york state shows persistence of human disease--associated listeria monocytogenes strains in retail environments.while there is considerable information available regarding listeria monocytogenes contamination patterns in food processing plants, our understanding of l. monocytogenes contamination and transmission in retail operations is limited. we characterized 125 food, 40 environmental, and 342 human clinical l. monocytogenes isolates collected in new york state from 1997 to 2002 using automated ribotyping and hly allelic variation. all environmental isolates were obtained from retail establishments and ...200415270495
survival of escherichia coli o157:h7, salmonella enteritidis, staphylococcus aureus, and listeria monocytogenes in kimchi.the survival of gram-positive and gram-negative foodborne pathogens in both commercial and laboratory-prepared kimchi (a traditional fermented food widely consumed in japan) was investigated. it was found that escherichia coli o157:h7, salmonella enteritidis, staphylococcus aureus, and listeria monocytogenes could survive in both commercial and laboratory-prepared kimchi inoculated with these pathogens and incubated at 10 degrees c for 7 days. however, when incubation was prolonged, the s. aureu ...200415270509
the bax pcr assay for screening listeria monocytogenes targets a partial putative gene lmo2234.the bax pcr for screening listeria monocytogenes is a commercial pcr assay for specifically targeting l. monocytogenes, a foodborne pathogen that can contaminate a variety of foods and cause a potentially fatal disease, listeriosis, among high-risk populations. the high specificity (> 98%) of this pcr assay is achieved by targeting a species-specific genomic region (approximately 400 bp) presumably found only in l. monocytogenes. in this study, the identity of the bax pcr-targeted genomic region ...200415270511
sequence and binding activity of the autolysin-adhesin ami from epidemic listeria monocytogenes 4b.ami is an autolytic amidase from listeria monocytogenes that is targeted to the bacterial surface via its c-terminal cell wall anchoring (cwa) domain. we recently showed that the cwa domain from ami of l. monocytogenes egd (serovar 1/2a) (ami 1/2a) mediated bacterial binding to mammalian cells. here we studied the sequence and binding properties of ami from chut 82337 (serovar 4b) (ami 4b). the ami 4b polypeptide is predicted to be 770 amino acids long (compared with the 917 amino acids of ami 1 ...200415271896
cellular pharmacokinetics and pharmacodynamics of the glycopeptide antibiotic oritavancin (ly333328) in a model of j774 mouse macrophages.the intracellular pharmacokinetics and pharmacodynamics of oritavancin (ly333328) were studied in cultured cells. oritavancin was avidly accumulated by j774 and thp-1 macrophages and rat fibroblasts and to a lesser extent by llc-pk1 and caco-2 cells. in j774 macrophages, the level of accumulation reached a plateau (at 370-fold the extracellular concentration) within 24 h and was partly defeated by a rise in serum protein levels. efflux was incomplete (with a plateau at two-thirds of the original ...200415273091
application of oligonucleotide array technology for the rapid detection of pathogenic bacteria of foodborne infections.a rapid and accurate method for detection for common pathogenic bacteria in foodborne infections was established by using oligonucleotide array technology. nylon membrane was used as the array support. a mutation region of the 23s rrna gene was selected as the discrimination target from 14 species (genera) of bacteria causing foodborne infections and two unrelated bacterial species. a pair of universal primers was designed for pcr amplification of the 23s rrna gene. twenty-one species (genera)-s ...200415279944
antimicrobial activity of reuterin in combination with nisin against food-borne pathogens.antimicrobial activity of reuterin individually or in combination with nisin against different food-borne gram-positive and gram-negative pathogens in milk was investigated. reuterin (8 au/ml) exhibited bacteriostatic activity against listeria monocytogenes, whereas its activity was slightly bactericidal against staphylococcus aureus at 37 degrees c. higher bactericidal activity was detected against escherichia coli o157:h7, salmonella choleraesuis subsp. choleraesuis, yersinia enterocolitica, a ...200415282134
changes in the immune functions and susceptibility to listeria monocytogenes infection in mice fed dietary lipids.the direct examination of the effects that fish oil diets (composed of long-chain n-3 polyunsaturated fatty acids) exert on immune system function indicates a reduction of host natural resistance to infectious diseases mainly because of a suppression of immune function generated by the fatty acids contained in this diet. here, we evaluated the concentration of il-12, il-4, prostaglandin e2 and leukotriene b4 in the serum from balb/c mice receiving four different diets. each group was fed a diet ...200415283846
thermal inactivation of salmonella and listeria monocytogenes in ground chicken thigh/leg meat and skin.thermal inactivation d and z values of salmonella and listeria monocytogenes were obtained for chicken thigh and leg meat and skin. the d values of salmonella at 55 to 70 degrees c were 43.33 to 0.07 min in the meat and 43.76 to 0.09 min in the skin. the d values of l. monocytogenes at 55 to 70 degrees c were 38.94 to 0.04 min in the meat and 34.05 to 0.05 min in the skin. the z value of salmonella was 5.34 degrees c in the meat and 5.56 degrees c in the skin. the z value of l. monocytogenes was ...200415285516
progress towards the use of listeria monocytogenes as a live bacterial vaccine vector for the delivery of hiv antigens.listeria monocytogenes is a facultative intracellular bacterium that enters the cell by phagocytosis after which it colonizes the cytosol of the host cell. it is thus a potent vaccine vector for the presentation of passenger antigens to the major histocompatability complex class ii and class i pathways of antigen processing and presentation. this article shall review the progress made in developing this unusual bacterium as a vaccine vector. in mouse models, recombinant listeria carrying a numbe ...200415285711
use of a fluorescent viability stain to assess lethal and sublethal injury in food-borne bacteria exposed to high-intensity pulsed electric fields.to apply scanning electron microscopy, image analysis and a fluorescent viability stain to assess lethal and sublethal in food-borne bacteria exposed to high-intensity pulsed electric fields (pef).200415287869
quantification of listeria monocytogenes in fermented sausages by mpn-pcr method.to combine the principles of most-probable-number (mpn) statistics and the conventional pcr technique to enumerate listeria monocytogenes in fermented sausages.200415287877
retrieving sequences of enzymes experimentally characterized but erroneously annotated : the case of the putrescine carbamoyltransferase.annotating genomes remains an hazardous task. mistakes or gaps in such a complex process may occur when relevant knowledge is ignored, whether lost, forgotten or overlooked. this paper exemplifies an approach which could help to resuscitate such meaningful data.200415287962
expression of mptc of listeria monocytogenes induces sensitivity to class iia bacteriocins in lactococcus lactis.sensitivity to class iia bacteriocins from lactic acid bacteria was recently associated with the mannose phosphotransferase system (pts) permease, in listeria monocytogenes. to assess the involvement of this protein complex in class iia bacteriocin activity, the mptacd operon, encoding, was heterologously expressed in an insensitive species, namely lactococcus lactis, using the nice double plasmid system. upon induction of the cloned operon, the recombinant lc. lactis became sensitive to leucoci ...200415289562
hypochlorite inactivation kinetics of listeria monocytogenes in phosphate buffer.the inactivation kinetics of listeria monocytogenes in a phosphate buffer (pb) was determined at different hypochlorite concentrations, ph values and temperatures. d-values, using a linear regression, of l. monocytogenes in pb (ph 6.5) were 23.54, 17.40, 14.24 and 12.00s at 5, 10, 50 and 100 mg l(-1) hypochlorite, respectively, at 30 degrees c. the k-values ranged from 0.098 to 0.192s(-1) and 0.007 to 0.018s(-1) for hypochlorite concentrations (from 5 to 100 mg l(-1)) in pb (ph 6.5) and pb conta ...200415293951
ecology and transmission of listeria monocytogenes infecting ruminants and in the farm environment.a case-control study involving 24 case farms with at least one recent case of listeriosis and 28 matched control farms with no listeriosis cases was conducted to probe the transmission and ecology of listeria monocytogenes on farms. a total of 528 fecal, 516 feed, and 1,012 environmental soil and water samples were cultured for l. monocytogenes. while the overall prevalence of l. monocytogenes in cattle case farms (24.4%) was similar to that in control farms (20.2%), small-ruminant (goat and she ...200415294773
mutational analysis of mesentericin y105, an anti-listeria bacteriocin, for determination of impact on bactericidal activity, in vitro secondary structure, and membrane interaction.mesentericin y105 is a 37-residue bacteriocin produced by leuconostoc mesenteroides y105 that displays antagonistic activity against gram-positive bacteria such as enterococcus faecalis and listeria monocytogenes. it is closely related to leucocin a, an antimicrobial peptide containing beta-sheet and alpha-helical structures. to analyze structure-function relationships and the mode of action of this bacteriocin, we generated a collection of mesentericin derivatives. mutations were obtained mostl ...200415294801
mechanisms regulating the positioning of mouse p47 resistance gtpases lrg-47 and iigp1 on cellular membranes: retargeting to plasma membrane induced by phagocytosis.the recently identified p47 gtpases are one of the most effective cell-autonomous resistance systems known against intracellular pathogens in the mouse. one member of the family, lrg-47, has been shown to be essential for immune control in vivo of listeria monocytogenes, toxoplasma gondii, mycobacterium tuberculosis, and mycobacterium avium, possibly by promoting acidification of the phagosome. however, the intracellular localization of lrg-47, and the nature of its association with the phagosom ...200415294976
cytoplasmic entry of listeria monocytogenes enhances dendritic cell maturation and t cell differentiation and function.protective immunity to the intracellular bacterial pathogen, listeria monocytogenes, is mediated by a vigorous t cell response. in particular, cd8(+) cytolytic t cells provide essential effector function in the clearance of bacterial infection. the cytoplasmic entry of listeria facilitated by listeriolysin o is an essential feature not only of the bacteria's virulence, but of the ability of the bacteria to elicit protective immunity in the host. to determine how cytoplasmic entry of listeria reg ...200415294981
[epidemiology of bacterial meningitis in france in 2002].in france, two sources of data, the mandatory notification and the hospital laboratory network epibac, allow the health authorities to monitor the incidence of bacterial meningitis. in 2002 around 1,500 cases were estimated in france. the more common microorganisms were: streptococcus pneumoniae 49% (incidence 1.19/100,000), neisseria meningitidis 33% (incidence 0.83) and streptococcus agalactiae (streptococcus b) 11% (incidence 0.27). listeria monocytogenes accounted for 4% of the cases (incide ...200415296270
differentiation of the major listeria monocytogenes serovars by multiplex pcr.a new multiplex pcr assay was developed to separate the four major listeria monocytogenes serovars isolated from food and patients (1/2a, 1/2b, 1/2c, and 4b) into distinct groups. the pcr test, which constitutes a rapid and practical alternative to laborious classical serotyping, was successfully evaluated with 222 listeria strains.200415297538
catabolite repression and virulence gene expression in listeria monocytogenes.previous studies have suggested that carbohydrates may affect expression of virulence genes in listeria monocytogenes. which carbohydrates influence virulence gene expression and how carbohydrates mediate expression, however, is not clear. the goal of this work was to examine how carbohydrates affect virulence gene expression in l. monocytogenes 10403s. growth studies were conducted in medium containing glucose and various sugars. metabolism of arbutin, arabitol, cellobiose, mannose, maltose, tr ...200415297913
cd4+ t cells are required for the maintenance, not programming, of memory cd8+ t cells after acute infection.immunization in the absence of cd4(+) t cell help results in defective cd8(+) t cell memory, deficient recall responses and diminished protective immunity. here we investigated at what stage during the immune response to pathogen cd4(+) t cells are essential in the promotion of functional cd8(+) t cell memory. memory cd8(+) t cell numbers decreased gradually in the absence of cd4(+) t cells despite the presence of similar numbers of memory cell precursors at the peak of the effector phase. adopt ...200415300249
mice lacking the type i interferon receptor are resistant to listeria monocytogenes.listeria monocytogenes is a facultative intracellular pathogen that induces a cytosolic signaling cascade resulting in expression of interferon (ifn)-beta. although type i ifns are critical in viral defense, their role in immunity to bacterial pathogens is much less clear. in this study, we addressed the role of type i ifns by examining the infection of l. monocytogenes in balb/c mice lacking the type i ifn receptor (ifn-alpha/betar-/-). during the first 24 h of infection in vivo, ifn-alpha/beta ...200415302899
type i interferon sensitizes lymphocytes to apoptosis and reduces resistance to listeria infection.infection with listeria monocytogenes causes lymphocyte apoptosis that is mediated by the actions of the pore-forming virulence factor listeriolysin o (llo). previous work showed that activated lymphocytes were highly sensitive to llo-induced apoptosis, whereas resting lymphocytes were less susceptible. we now show that mice deficient in the type i interferon (ifn) receptor were more resistant to listeria infection and had less apoptotic lesions than wild-type counterparts. furthermore, treatmen ...200415302900
type i interferon production enhances susceptibility to listeria monocytogenes infection.numerous bacterial products such as lipopolysaccharide potently induce type i interferons (ifns); however, the contribution of this innate response to host defense against bacterial infection remains unclear. although mice deficient in either ifn regulatory factor (irf)3 or the type i ifn receptor (ifnar)1 are highly susceptible to viral infection, we show that these mice exhibit a profound resistance to infection caused by the gram-positive intracellular bacterium listeria monocytogenes compare ...200415302901
listeria monocytogenes regulates flagellar motility gene expression through mogr, a transcriptional repressor required for virulence.previous studies have shown that listeria monocytogenes flagellar motility genes, including flaa, encoding flagellin, are transcriptionally down-regulated at 37 degrees c. for some l. monocytogenes strains, temperature-dependent motility gene expression is less stringent. by using flaa-lacz transcriptional fusions, we identified regions upstream of the -35/-10 promoter elements that are necessary for temperature-dependent expression of flaa in l. monocytogenes strain egde. whereas the sequence o ...200415302931
antibody-mediated activation of the classical pathway of complement may compensate for mannose-binding lectin deficiency.deficiency of mannose-binding lectin (mbl), a recognition molecule of the lectin pathway of complement, is associated with increased susceptibility to infections. the high frequency of mbl deficiency suggests that defective mbl-mediated innate immunity can be compensated by alternative defense strategies. to examine this hypothesis, complement activation by mbl-binding ligands was studied. the results show that the prototypic mbl ligand mannan can induce complement activation via both the lectin ...200415307191
nationwide survey of human listeria monocytogenes infection in japan.listeriosis, caused by listeria monocytogenes, is a significant public-health concern as a result of its clinical severity and high mortality. large foodborne outbreaks of listeriosis have occurred during the last two decades in europe and the united states, but to date there have been no food-mediated epidemics of the disease and very little information is available on the number of cases of listeriosis in japan. we performed a nationwide surveillance study of listeriosis. the data were collect ...200415310181
characteristics of the gastritis induced by listeria monocytogenes in mice: microbiology, histopathology, and mrna expression of inflammatory mediators with time course of infection.listeria monocytogenes induces the suppurative gastritis in some mice strains. in this study, characteristics of the gastritis caused by l. monocytogenes infection in mice were examined with time course of infection. mice were administered intragastrically with 1.8 x 10(8) cfu of l. monocytogenes. each three mice were sacrificed by cervical dislocation at 1, 3, 5, 7, 10, 14, 17, 21, and 28 days postinoculation (pi), respectively. bacterial colonization in the stomachs reached the peak at 3 days ...200415312848
cd4+cd25+ regulatory t cells that secrete tgfbeta and il-10 are preferentially induced by a vaccine vector.cd4cd25 t cells generated in a vaccine scenario can play a critical role in limiting antitumor therapy, thus having widespread implications for the immunotherapy-based treatment of cancer. the authors previously used listeria monocytogenes to develop two vaccine constructs that express hpv-16 e7 protein and induce strong cellular immunity to hpv-e7-expressing tumors. immunization of mice bearing established e7-expressing tumors with lm-llo-e7 induced regression of the tumors, whereas lm-e7 showe ...200415314542
Refresh
firstprev
...81828384858687888990...
nextlast
Displaying items 8001 - 8100 of 16792