Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| synthesis and biological evaluation of a new series of benzimidazole derivatives as antimicrobial, antiquorum-sensing and antitumor agents. | new benzimidazole derivatives were synthesized and assessed for antimicrobial efficacy toward escherichia coli, bacillus cereus, staphylococcus aureus, candida albicans and aspergillus fumigatus 293. results indicated that compounds 3c and 3n have promising activity toward s. aureus, whereas 3i exhibited remarkable efficacy toward b. cereus. moreover, compound 3c was proved to be the most active antifungal analog toward c. albicans. on the other hand, 3n displayed the highest activity against a. ... | 2017 | 28334654 |
| genotypic heterogeneity of emetic toxin producing bacillus cereus isolates from china. | emetic toxin-producing bacillus cereus (emetic b. cereus) is the third member of b. cereus group whose toxins are encoded by megaplasmids, beside anthrax and insecticidal toxins of b. anthracis and b. thuringiensis, respectively. a total of 18 emetic isolates collected from food poisoning events, clinical and non-random food samples in zhejiang province of china, were analyzed by plasmid screening, pulse field gel electrophoresis, multilocus sequence typing, and toxic gene identification to inve ... | 2017 | 27744366 |
| a four-gene operon in bacillus cereus produces two rare spore-decorating sugars. | bacterial glycan structures on cell surfaces are critical for cell-cell recognition and adhesion and in host-pathogen interactions. accordingly, unraveling the sugar composition of bacterial cell surfaces can shed light on bacterial growth and pathogenesis. here, we found that two rare sugars with a 3-c-methyl-6-deoxy-hexose structure were linked to spore glycan in bacillus cereus atcc 14579 and atcc 10876. moreover, we identified a four-gene operon in b. cereus atcc 14579 that encodes proteins ... | 2017 | 28298443 |
| molecular tiling on the surface of a bacterial spore - the exosporium of the bacillus anthracis/cereus/thuringiensis group. | bacteria of the genera bacillus and clostridium form highly resistant spores, which in the case of some pathogens act as the infectious agents. an exosporium forms the outermost layer of some spores; it plays roles in protection, adhesion, dissemination, host targeting in pathogens and germination control. the exosporium of the bacillus cereus group, including the anthrax pathogen, contains a 2d-crystalline basal layer, overlaid by a hairy nap. bcla and related proteins form the hairy nap, and r ... | 2017 | 28214340 |
| evaluating bionanoparticle infused fungal metabolites as a novel antimicrobial agent. | therapeutic properties of fungal metabolites and silver nanoparticles have been well documented. while fungal metabolites have been used for centuries as medicinal drugs, potential of biogenic silver nanoparticles has recently received attention. we have evaluated the antimicrobial potential of aspergillus terreus crude extract, silver nanoparticles and an amalgamation of both against four pathogenic bacterial strains. antimicrobial activity of the following was evaluated - a. terreus extract, b ... | 2017 | 27429931 |
| bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides. | several products of industrial interest are produced by bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. this study aimed to investigate the potential of two bacterial isolates (p5 and c3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. phylogenetic analyses showed close relation of isolates p5 and c3 with bacillus amyloliquefaciens and bacillus thuringie ... | 2017 | 28197131 |
| dynamics of bacterial class bacilli in the deepest valley lake of kashmir-the manasbal lake. | in recognition of the importance of bacteria as ecological indicators of the aquatic systems a comprehensive and systematic analysis was carried out on manasbal lake, the deepest spring fed valley lake of kashmir. the main objective envisaged was to analyze bacterial community composition (bcc) and for this purpose systematic and regular sampling of waters from ten different sampling stations, predetermined in the lake according to differences in degree of human interference and also as zones of ... | 2017 | 28087491 |
| evaluation of bacillus cereus and bacillus pumilus metabolites for anthelmintic activity. | to assess the anthelmintic acivity of bacillus cereus and bacillus pumilus metabolites. | 2017 | 25598639 |
| impact of bacillus cereus nrkt on grape ripe rot disease through resveratrol synthesis in berry skin. | vine growers are faced with the difficult problem of how to control grape ripe rot disease in vineyards because of fear of accumulation of pesticide residues on grape berries near harvest. biological control is an alternative non-hazardous technique to control the diseases. | 2017 | 27038426 |
| [functional analysis of grp and iris, the gag and env domesticated errantivirus genes, in the drosophila melanogaster genome]. | drosophila melanogaster is the only invertebrate that contains endogenous retroviruses, which are called errantiviruses. two domesticated genes, grp and iris, which originate from errantivirus gag and env, respectively, have been found in the d. melanogaster genome. the functions performed by the genes in drosophila are still unclear. to identify the functions of domesticated gag and env in the d. melanogaster genome, expression of iris and grp was studied in strains differing by the presence or ... | 2017 | 27414781 |
| changes in microbial composition and the prevalence of foodborne pathogens in crab marinated in soy sauce produced by six manufacturing plants. | the present study examined the changes in microbiological composition during the production process of crab marinated in soy sauce, potential microbial hazards, potential contamination routes and effective critical control points. crab and soy sauce samples were obtained from six different manufacturing plants at different stages, and their microbiological content was comprehensively assessed by quantitative and qualitative analyses. | 2017 | 27469473 |
| biosynthesis of silver nanoparticles by novosphingobium sp. thg-c3 and their antimicrobial potential. | the present study described biosynthesis of silver nanoparticles (agnps) using a bacterial strain novosphingobium sp. thg-c3, isolated from soil, and their application in antibacterial activity. the maximum absorbance values of the synthesized agnps was measured at 406 nm in ultraviolet-visible spectrophotometry and were mostly spherical in shape with particle size in range of 8-25 nm by field emission transmission electron microscopy analysis. x-ray diffraction pattern corresponding to planes ( ... | 2017 | 27145847 |
| evaluation of health potential of nutritionally enriched kodo millet (eleusine coracana) grown in himachal pradesh, india. | in this study, kodo millet grains were phytochemically investigated for their nutritional and antioxidant potential for their use as functional foods. methanolic extracts of grains showed higher phenolic content and antioxidant activity. tlc studies of the extracted polyphenols from kodo millet showed the predominant presence of ferulic acid and cinnamic acid in the millet. further quantification of these polyphenols was done by using hplc, analysing ferulic acid and cinnamic acid. antagonistic ... | 2017 | 27507461 |
| factors of detection of bacillus cereus strains in eye cream. | bacillus cereus has been associated with clinical infections and is also the cause of post-traumatic endophthalmitis as well as endogenous eye infections, which can result in blindness. cosmetics, although preserved, can be contaminated during manufacture or use and thus cause serious health issues. | 2017 | 27578165 |
| chemical composition and in vitro evaluation of antimicrobial, antioxidant and antigerminative properties of the seed oil from the tunisian endemic ferula tunetana pomel ex batt. | essential oil of the seeds from the endemic tunisian plant ferula tunetana pomel ex batt. was analyzed for its chemical composition and screened for its antimicrobial, antioxidant and antigerminative properties. the chemical composition of the isolated oil is reported for the first time. according to the gc/fid, gc/ms and (13) c-nmr analysis results; 18 compounds were identified accounting for 84.6% of the total oil. the chemical composition of this essential oil was characterized by the presenc ... | 2017 | 27638478 |
| bacillus cereus causing intratumoral brain abscess. | we report a case of intratumoral brain abscess due to bacillus cereus in an adult male patient, which was managed successfully with excision of lesion and piperacillin-tazobactam for the duration of 5 weeks. to the best of our knowledge, this is a first case report of b. cereus infection leading to intratumoral brain abscess in a patient with a history of steroid administration by the intravenous route. | 2017 | 27721298 |
| biochemical and biological characterization of two brassicaceae after their commercial expiry date. | two brassicaceae (eruca sativa, brassica oleracea var. sabauda) were stored in air and under a modified atmosphere for several days after their expiry date and then analyzed. the polyphenol content and composition, as well as the antioxidant activity of the extracts, were assessed, compared to the fresh products. antimicrobial properties on tester strains (bacillus cereus, escherichia coli, staphylococcus aureus and pseudomonas aeruginosa) and in vitro anti-proliferative activity were evaluated. ... | 2017 | 27719918 |
| the thnr gene is a negative transcription regulator of the thurincin h genetic cassette in bacillus thuringiensis subsp. morrisoni. | thurincin h is a bacteriocin synthesized by some strains of bacillus thuringiensis. in this study, the thurincin h genetic cassette, which contains ten genes, from a mexican strain of b. thuringiensis subsp. morrisoni (btm) was cloned and sequenced. to study the function of the thnr gene component in the cassette, we generated various constructs with or without thnr for expression in btm. all transformants harboring thnr in recombinant plasmids showed a decrease of ~15 to ~90 % in inhibitory act ... | 2017 | 27699438 |
| non-hemolytic enterotoxin of bacillus cereus induces apoptosis in vero cells. | bacillus cereus is an opportunistic pathogen that often causes foodborne infectious diseases and food poisoning. non-hemolytic enterotoxin (nhe) is the major toxin found in almost all enteropathogenic b. cereus and b. thuringiensis isolates. however, little is known about the cellular response after nhe triggered pore formation on cell membrane. here, we demonstrate that nhe induced cell cycle arrest at g0 /g1 phase and provoked apoptosis in vero cells, most likely associated with mitogen-activa ... | 2017 | 27762484 |
| synthesis and antibacterial evaluation of new n- and s-glycosides analogues with dinitrophenyl-substituted heterocyclic bases. | four n- and s-glycosides 13-16 having nucleobases 7-12 binding to sugar molecules from one side and to 3,5-dinitrophenyl moieties from another side were synthesized from 3,5-dinitrobenzoic acid 2. the synthetic intermediates, hydrazide 5 and thiosemicarbazide 6 regarded as important key compounds for the synthesis of nucleobases 7-12, each was obtained by two approaches. structures of synthesized compounds were determined spectroscopically. antibacterial activities for synthetic intermediates an ... | 2017 | 27761763 |
| raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. | microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ... | 2017 | 27747902 |
| itraq-based proteomic analysis of li-f type peptides produced by paenibacillus polymyxa jsa-9 mode of action against bacillus cereus. | li-f type peptides (amp-jsa9) produced by paenibacillus polymyxa jsa-9 are a group of cyclic lipodepsipeptide antibiotics that exhibit a broad antimicrobial spectrum against gram-positive bacteria and filamentous fungi, especially bacillus cereus and fusarium moniliforme. in this study, to better understand the antibacterial mechanism of amp-jsa9 against b. cereus, the ultrastructure of amp-jsa9-treated b. cereus cells was observed by both atomic force microscopy and transmission electron micros ... | 2017 | 27609309 |
| co-precipitation synthesis and characterization of co doped sno2 nps, hsa interaction via various spectroscopic techniques and their antimicrobial and photocatalytic activities. | sn1-xcoxo2 (x=0.00, 0.01, 0.03, 0.05) nanoparticles (nps) of average size ∼30-40nm were synthesized by co-precipitation method. the interaction of co doped sno2 nps with human serum albumin (hsa) and their photocatalytic and antimicrobial properties were studied. the structural analysis and morphology of co doped sno2 nps were analysed via x-ray diffraction (xrd), scanning electron microscopy (sem), energy dispersive x-ray spectroscopy (eds), high resolution transmission electron microscopy (hrt ... | 2017 | 27771412 |
| effect of surfactants and inducers on increased uricase production under submerged fermentations by bacillus cereus. | uricase is a clinical enzyme used for the oxidation of uric acid crystals in gout disease. the present study aimed to increase the suitable surfactant-mediated uricase production on induction by different concentrations of inducers. the efficiency of bacillus cereus to produce extracellular uricase enzyme was studied in uric acid-containing agar plates. among the studied inducers, uric acid is the potential inducer for uricase production under submerged fermentations (smf), which induced 19.41 u ... | 2017 | 27071031 |
| evidence of mercury trapping in biofilm-eps and mer operon-based volatilization of inorganic mercury in a marine bacterium bacillus cereus bw-201b. | biofilm-forming mercury-resistant marine bacterium bacillus cereus bw-201b has been explored to evident that the bacterial biofilm-eps (exopolymers) trap inorganic mercury but subsequently release eps-bound mercury for induction of mer operon-mediated volatilization of inorganic mercury. the isolate was able to tolerate 50 ppm of mercury and forms biofilm in presence of mercury. mer operon-mediated volatilization was confirmed, and -sh was found to be the key functional group of bacterial eps re ... | 2017 | 27815566 |
| disruptions of the genes involved in lysine biosynthesis, iron acquisition, and secondary metabolisms affect virulence and fitness in metarhizium robertsii. | based on genomic analysis, polyketide synthase (pks) and nonribosomal peptide synthetase (nrps) pathways account for biosynthesis of the majority of the secondary metabolites produced by the entomopathogenic fungus metarhizium robertsii. to evaluate the contribution of these pathways to m. robertsii fitness and/or virulence, mutants deleted for mrppta, the sfp-type 4' phosphopantetheinyl transferase gene required for their activation were generated. δmrppta strains were deficient in pks and nrps ... | 2017 | 27876630 |
| characterization of three autolysins with activity against cereulide-producing bacillus isolates in food matrices. | bacillus cereus is a pathogen related with diarrhoeal or emetic food poisoning cases, of which the latter caused by the cereulide-producing isolates are more severe with several reported lethal cases. it is therefore necessary to develop an effective strategy to prevent the propagation of b. cereus in the food supply. in this study, three autolysins from the cereulide-producing b. cereus group isolates, lysis075, lysf8819.1 and lyscer057, were identified and characterized. the results showed tha ... | 2017 | 27835772 |
| bacillus cereus strain isolated from demodex folliculorum in patients with topical steroid-induced rosaceiform facial dermatitis. | the aim of the study was to identify bacillus species from the demodex folliculorum of patients with topical steroidinduced facial rosaceiform dermatitis. of the 75 patients examined, 20% had clinical spinulosis, while 18.66% had dermoscopic features of demodex: follicular plugs and tails. of the 17.33% positive patients identified upon microscopy for demodex, samples for bacterial culture were plated on trypticase soy colombia agar. identification was performed by microorganisms grown method ma ... | 2017 | 27828651 |
| multilocus sequence type profiles of bacillus cereus isolates from infant formula in china. | bacillus cereus sensu stricto is an opportunistic foodborne pathogen. the multilocus sequence type (mlst) of 74 b. cereus isolated from 513 non-random infant formula in china was analyzed. of 64 sequence types (sts) detected, 50 sts and 6 alleles were newly found in pubmlst database. all isolates except for one singleton (st-1049), were classified into 7 clonal complexes (cc) by burst (n-4), in which cc1 with core ancestral clone st-26 was the largest group including 86% isolates, and cc2, 3, 9, ... | 2017 | 27889164 |
| microbial decontamination of onion powder using microwave-powered cold plasma treatments. | the effects of microwave-integrated cold plasma (cp) treatments against spores of bacillus cereus and aspergillus brasiliensis and escherichia coli o157:h7 on onion powder were investigated. the growth of b. cereus, a. brasiliensis, and e. coli o157:h7 in the treated onion powder was assessed during storage at 4 and 25 °c, along with the physicochemical and sensory properties of the powder. onion powder inoculated with b. cereus was treated with cp using helium as a plasma-forming gas, with simu ... | 2017 | 27889137 |
| analysis of germination capacity and germinant receptor (sub)clusters of genome-sequenced bacillus cereus environmental isolates and model strains. | spore germination of 17 bacillus cereus food isolates and reference strains was evaluated using flow cytometry analysis in combination with fluorescent staining at a single-spore level. this approach allowed for rapid collection of germination data under more than 20 conditions, including heat activation of spores, germination in complex media (brain heart infusion [bhi] and tryptone soy broth [tsb]), and exposure to saturating concentrations of single amino acids and the combination of alanine ... | 2017 | 27881417 |
| effects of bacillus cereus endospores on free-living protist growth. | we studied the predator-prey interactions between heterotrophic protists and endospores of bacillus cereus group bacteria, in order to gain insight on survival and dispersal of b. cereus endospores in the environment. it has been hypothesised that the spore stage protects against digestion by predating protists. therefore, experiments were carried out to investigate the impact of b. cereus endospores and vegetative cells, as the only food source, on individual amoeboid, flagellated and ciliated ... | 2017 | 27928597 |
| antibacterial, antioxidant and anti-proliferative properties and zinc content of five south portugal herbs. | crataegus monogyna l. (rosaceae) (cm), equisetum telmateia l. (equisataceae) (et), geranium purpureum vil. (geraniaceae) (gp), mentha suaveolens ehrh. (lamiaceae) (ms), and lavandula stoechas l. spp. luisieri (lamiaceae) (ls) are all medicinal. | 2017 | 27925492 |
| one-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic atp regeneration system. | in vitro cascade catalysis using enzyme-based system is becoming a promising biomanufacturing platform for biofuels and biochemicals production. glutathione is a pivotal non-protein thiol compound and widely applied in food and pharmaceutical industries. in this study, glutathione was synthesized by a bifunctional glutathione synthetase together with a thermophilic atp regeneration system through a two-enzyme cascade in vitro. four bifunctional glutathione synthetases from streptococcus sanguini ... | 2017 | 27919691 |
| termitarium-inhabiting bacillus spp. enhanced plant growth and bioactive component in turmeric (curcuma longa l.). | curcumin (diferuloyl methane) is the main bioactive component of turmeric (curcuma longa l.) having remarkable multipotent medicinal and therapeutic applications. two bacilli isolated from termitarium soil and identified as bacillus endophyticus tsh42 and bacillus cereus tsh77 were used for bacterization of rhizome for raising c. longa ver. suguna for growth and enhancement. both the strains showed remarkable pgp activities and also chemotactic in nature with high chemotactic index. turmeric pla ... | 2017 | 27913879 |
| screening of mercury-resistant and indole-3-acetic acid producing bacterial-consortium for growth promotion of cicer arietinum l. | mercury resistant (hg(r) ) bacteria were screened from industrial effluents and effluents-polluted rhizosphere soils near to districts kasur and sheikhupura, pakistan. out of 60 isolates, three bacterial strains, bacillus sp. az-1, bacillus cereus az-2, and enterobacter cloacae az-3 showed hg-resistance as 20 μg ml(-1) of hgcl2 and indole-3-acetic acid (iaa) production as 8-38 μg ml(-1) . biochemical and molecular characterization of selected bacteria was confirmed by 16s ribotyping. mercury res ... | 2017 | 27911010 |
| hygienic characteristics and microbiological hazard identification in horse and donkey raw milk. | today the interest toward horse (equus caballus) and donkey (equus asinus) milk for human consumption is receiving a renewed attention because of its particular composition, hypoallergenicity, and nutraceutical properties. the realistic perspective of global use of this aliment in balanced diets, especially for infancy and geriatrics, poses the need for a more in depth knowledge on milk hygiene and on the health status of dairy animals, as a prerequisite of consumers' safety. the aim of this pap ... | 2017 | 27033528 |
| investigation on antimicrobial agents of the terrestrial streptomyces sp. bcc71188. | the terrestrial actinomycete strain bcc71188 was identified as streptomyces by its morphology (having spiral chain spore on the aerial mycelium), chemotaxonomy (containing ll-diaminopimelic acid in the cell wall), and 16s rrna gene sequence analysis [showing high similarity values compared with streptomyces samsunensis m1463(t) (99.85 %) and streptomyces malaysiensis nbrc 16446(t) (99.40 %)]. the crude extract exhibited antimalarial against plasmodium falciparum (ic50 0.19 μg/ml), anti-tb agains ... | 2017 | 27554496 |
| microbiological isolates and antibiotic susceptibilities: a 10-year review of culture-proven endophthalmitis cases. | to review the microbiological spectrum and antibiotic sensitivities of the pathogens that cause culture-proven endophthalmitis and to understand the status and trends of antibiotic susceptibility at a public hospital over a 10-year period. | 2017 | 27347700 |
| an in silico approach to understand the structure-function properties of a serine protease (bacifrinase) from bacillus cereus and experimental evidence to support the interaction of bacifrinase with fibrinogen and thrombin. | microbial fibrinogenolytic serine proteases find therapeutic applications in the treatment of thrombosis- and hyperfibrinogenemia-associated disorders. however, analysis of structure-function properties of an enzyme is utmost important before its commercial application. in this study, an attempt has been made to understand the structure of a fibrinogenolytic protease enzyme, "bacifrinase" from bacillus cereus strain ab01. from the molecular dynamics trajectory analysis, the modelled three-dimens ... | 2017 | 26919276 |
| risk factors for post-traumatic endophthalmitis in patients with positive intraocular cultures. | to determine the risk factors for post-traumatic endophthalmitis in patients with positive intraocular culture. | 2017 | 28221562 |
| effect of microorganisms on reducing cadmium uptake and toxicity in rice (oryza sativa l.). | this study analyzed the application of three microorganism inoculums, including bacillus subtilis, bacillus cereus, and commercial effective microorganism (em) solution in order to determine cadmium (cd) reduction in rice (oryza sativa l.) and rice growth promotion. rice was grown in cd-contaminated soil (120 mg/kg) and selected microorganisms were inoculated. cd concentration and rice weight were measured at 45 and 120 days of the experiment. the result showed that b. subtilis inoculation into ... | 2017 | 28480489 |
| isolation and characterization of lactic acid bacteria from traditional pickles of himachal pradesh, india. | a total of 15 isolates of lactic acid bacteria (lab) were isolated from traditional pickles of rural and urban areas of himachal pradesh. these isolates were identified as enterococcus faecalis (7), lactobacillus plantarum (3), pediococcus pentosaceus (2), leuconostoc mesenteroides (1), lactococcus lactis (1) and enterococcus sp. (1) on the basis of morphological, biochemical and 16s rdna sequence analysis. all the labs produced exopolysaccharides, have antibiotic susceptibility, and possess act ... | 2017 | 28720951 |
| pcr denaturing gradient gel electrophoresis as a useful method to identify of intestinal bacteria flora in haemaphysalis flava ticks. | ticks are blood-sucking ectoparasites of great medical and veterinary significance that can transmit bacteria, protozoa, fungi and viruses, causing a variety of human and animal diseases worldwide. in the present study, the intestinal bacterial flora associated with haemaphysalis flava ticks in different developmental stages were analyzed using polymerase chain reaction (pcr) and denaturing gradient gel electrophoresis (dgge). eleven distinct dgge bands were found using pcr-dgge method. sequence ... | 2017 | 28426403 |
| sensitive detection of staphylococcus aureus with vancomycin-conjugated magnetic beads as enrichment carriers combined with flow cytometry. | a novel sandwich strategy was designed to detect staphylococcus aureus. the strategy is based on an antibacterial agent that captures bacterial cells and a fluorescein-labeled antibody that acts as the signal-output probe. vancomycin (van), which exerts a strong antibacterial effect on gram-positive bacteria, was utilized as a molecular recognition agent to detect pathogenic bacteria. to effectively concentrate s. aureus, we used bovine serum albumin (bsa) as the amplification carrier to modify ... | 2017 | 28590745 |
| production and characterization of a new biodegradable fenugreek seed gum based active nanocomposite film reinforced with nanoclays. | in the present work, fenugreek seed gum (fsg)/clay nanocomposite films were prepared with nanoclays (na(+) montmorillonite [mmt], halloysite [hnt] and nanomer(®) i.44 p [nm]) at different amounts (0, 2.5, 5.0 and 7.5g clay/100g fsg) by solution casting method and characterized. increasing amount of nanoclay significantly (p<0.05) improved oxygen barrier and thermal properties of the biodegradable films. agar diffusion tests revealed that fsg based nanocomposite films exhibited strong antimicrobi ... | 2017 | 28536016 |
| development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens. | bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ... | 2017 | 28505584 |
| antibacterial effects of lactobacillus isolates of curd and human milk origin against food-borne and human pathogens. | this study was undertaken to assess the antibacterial efficacy of lactobacilli isolated from curd and human milk samples. identities of thirty-one different lactobacilli (20 from curd and 11 from human milk) were confirmed by genus-specific pcr and 16s rrna-based sequencing. these strains belonged to five species, lactobacillus casei, l. delbrueckii, l. fermentum, l. plantarum, and l. pentosus. antibacterial activities of cell-free supernatants (cfss) of all the lactobacillus isolates were estim ... | 2017 | 28401466 |
| persistent anthrax as a major driver of wildlife mortality in a tropical rainforest. | anthrax is a globally important animal disease and zoonosis. despite this, our current knowledge of anthrax ecology is largely limited to arid ecosystems, where outbreaks are most commonly reported. here we show that the dynamics of an anthrax-causing agent, bacillus cereus biovar anthracis, in a tropical rainforest have severe consequences for local wildlife communities. using data and samples collected over three decades, we show that rainforest anthrax is a persistent and widespread cause of ... | 2017 | 28770842 |
| mosquito microbiome dynamics, a background for prevalence and seasonality of west nile virus. | symbiotic microbial communities augment host phenotype, including defense against pathogen carriage and infection. we sampled the microbial communities in 11 adult mosquito host species from six regions in southern ontario, canada over 3 years. of the factors examined, we found that mosquito species was the largest driver of the microbiota, with remarkable phylosymbiosis between host and microbiota. seasonal shifts of the microbiome were consistently repeated over the 3-year period, while region ... | 2017 | 28421042 |
| the antibacterial and antifungal activity of six essential oils and their cyto/genotoxicity to human hel 12469 cells. | six essential oils (from oregano, thyme, clove, lavender, clary sage, and arborvitae) exhibited different antibacterial and antifungal properties. antimicrobial activity was shown against pathogenic (escherichia coli, salmonella typhimurium, yersinia enterocolitica, staphylococcus aureus, listeria monocytogenes, and enterococcus faecalis) and environmental bacteria (bacillus cereus, arthrobacter protophormiae, pseudomonas fragi) and fungi (chaetomium globosum, penicillium chrysogenum, cladospori ... | 2017 | 28811611 |
| synthesis, antimicrobial, antiquorum-sensing and antitumor activities of new benzimidazole analogs. | new benzimidazole analogs were prepared and tested for antimicrobial efficacy toward escherichia coli atcc 12435, bacillus cereus uw 85, staphylococcus aureus atcc 29213, candida albicans and aspergillus fumigatus 293. results indicated that compound 10 has potent and broad spectrum antimicrobial activity. in addition, 4b and 5c showed eminent antimicrobial efficacy toward b. cereus, s. aureus, c. albicans and a. fumigatus. furthermore, 12 and 14 demonstrated interesting antifungal activity towa ... | 2017 | 28623814 |
| antimicrobial activity and chemical composition of brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide. | brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. however, studies on the antimicrobial activity of brunfelsia uniflora flower oleoresin have not been found yet. this study aimed to evaluate the chemical composition and antimicrobial activity of b. uniflora flower oleoresin obtained by supercritical carbon dioxide. oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition ... | 2017 | 28407186 |
| biological characterization of the biocontrol agent bacillus amyloliquefaciens cpa-8: the effect of temperature, ph and water activity on growth, susceptibility to antibiotics and detection of enterotoxic genes. | this work focuses on the biological understanding of the biocontrol agent bacillus amyloliquefaciens cpa-8 in order to accomplish the characterization required in the registration process for the development of a microorganism-based product. the tolerance of cpa-8 to grow under different ph-temperature and water activity (a w)-temperature conditions was widely demonstrated. regarding the ph results, optimum growth at the evaluated conditions was observed at 37 °c and ph between 7 and 5. on the c ... | 2017 | 28664219 |
| contribution of native phosphorous-solubilizing bacteria of acid soils on phosphorous acquisition in peanut (arachis hypogaea l.). | the present investigation analyzes the in vitro p solubilization [ca-p, al-p, fe(ii)-p, and fe(iii)-p] efficiency of native psb strains from acid soils of odisha and exploitation of the same through biofertilization in peanut (arachis hypogaea l.) growth and p acquisition. one hundred six numbers of soil samples with ph ≤ 5.50 were collected from five districts of odisha viz., balasore, cuttack, khordha, keonjhar, and mayurbhanj. one bacterial isolate from each district were selected and analyze ... | 2017 | 28455550 |
| structural and immunochemical relatedness suggests a conserved pathogenicity motif for secondary cell wall polysaccharides in bacillus anthracis and infection-associated bacillus cereus. | bacillus anthracis (ba) and human infection-associated bacillus cereus (bc) strains bc g9241 and bc 03bb87 have secondary cell wall polysaccharides (scwps) comprising an aminoglycosyl trisaccharide repeat: →4)-β-d-manpnac-(1→4)-β-d-glcpnac-(1→6)-α-d-glcpnac-(1→, substituted at glcnac residues with both α- and β-galp. in bc g9241 and bc 03bb87, an additional α-galp is attached to o-3 of mannac. using nmr spectroscopy, mass spectrometry and immunochemical methods, we compared these structures to s ... | 2017 | 28832613 |
| draft genome sequences from a novel clade of bacillus cereus sensu lato strains, isolated from the international space station. | the draft genome sequences of six bacillus strains, isolated from the international space station and belonging to the bacillus anthracis-b. cereus-b. thuringiensis group, are presented here. these strains were isolated from the japanese experiment module (one strain), u.s. harmony node 2 (three strains), and russian segment zvezda module (two strains). | 2017 | 28798168 |
| comparative genomics of bacillus thuringiensis reveals a path to specialized exploitation of multiple invertebrate hosts. | understanding the genetic basis of host shifts is a key genomic question for pathogen and parasite biology. the bacillus cereus group, which encompasses bacillus thuringiensis and bacillus anthracis, contains pathogens that can infect insects, nematodes, and vertebrates. since the target range of the essential virulence factors (cry toxins) and many isolates is well known, this group presents a powerful system for investigating how pathogens can diversify and adapt to phylogenetically distant ho ... | 2017 | 28790205 |
| non-toxin-producing bacillus cereus strains belonging to the b. anthracis clade isolated from the international space station. | in an ongoing microbial observatory investigation of the international space station (iss), 11 bacillus strains (2 from the kibo japanese experimental module, 4 from the u.s. segment, and 5 from the russian module) were isolated and their whole genomes were sequenced. a comparative analysis of the 16s rrna gene sequences of these isolates showed the highest similarity (>99%) to the bacillus anthracis-b. cereus-b. thuringiensis group. the fatty acid composition, polar lipid profile, peptidoglycan ... | 2017 | 28680972 |
| milk-originated bacillus cereus sensu lato strains harbouring bacillus anthracis-like plasmids are genetically and phenotypically diverse. | bacillus cereus sensu lato is widely distributed in food products, including raw and processed milk. plasmids often determine bacterial virulence and toxicity, but their role in the evolution of b. cereus sensu lato is only partly known. here, we observed that nearly 8% of b. cereus sensu lato isolates were positive for pxo1-like plasmids and 12% for pxo2-like plasmids in raw and ultra-heat-treated (uht) milk from one dairy plant. however, pxo1-like plasmids were significantly more frequent in r ... | 2017 | 28648290 |
| in defense of bacillus thuringiensis, the safest and most successful microbial insecticide available to humanity - a response to efsa. | the bacillus cereus group contains vertebrate pathogens such as bacillus anthracis and bacillus cereus and the invertebrate pathogen bacillus thuringiensis. microbial biopesticides based on b. thuringiensis (bt) are widely recognized as being among the safest and least environmentally damaging insecticidal products available. nevertheless, a recent food poisoning incident prompted a european food safety authority review which argued that b. thuringiensis poses a health risk equivalent to b. cere ... | 2017 | 28645183 |
| assembly and function of the bacillus anthracis s-layer. | bacillus anthracis, the anthrax agent, is a member of the bacillus cereus sensu lato group, which includes invasive pathogens of mammals or insects as well as nonpathogenic environmental strains. the genes for anthrax pathogenesis are located on two large virulence plasmids. similar virulence plasmids have been acquired by other b. cereus strains and enable the pathogenesis of anthrax-like diseases. among the virulence factors of b. anthracis is the s-layer-associated protein bsla, which endows ... | 2017 | 28622090 |
| recombinant protein expression, crystallization, and biophysical studies of a bacillus-conserved nucleotide pyrophosphorylase, bcmazg. | to overcome safety restrictions and regulations when studying genes and proteins from true pathogens, their homologues can be studied. bacillus anthracis is an obligate pathogen that causes fatal inhalational anthrax. bacillus cereus is considered a useful model for studying b. anthracis due to its close evolutionary relationship. the gene cluster ba1554 - ba1558 of b. anthracis is highly conserved with the bc1531- bc1535 cluster in b. cereus, as well as with the bt1364-bt1368 cluster in bacillu ... | 2017 | 28570540 |
| draft genome sequences of bacillus cereus e41 and bacillus anthracis f34 isolated from algerian salt lakes. | two strains of bacillus, b. cereus e41 and b. anthracis f34, were isolated from a salt lake in aïn m'lila-oum el bouaghi, eastern algeria, and ain baida-ouargla, southern algeria, respectively. their genomes display genes for the production of several bioactive secondary metabolites, including polyhydroxyalkanoate, iron siderophores, lipopeptides, and bacteriocins. | 2017 | 28522726 |
| draft genome sequence of bacillus cereus la2007, a human-pathogenic isolate harboring anthrax-like plasmids. | we present the genome sequence of bacillus cereus la2007, a strain isolated in 2007 from a fatal pneumonia case in louisiana. sequence-based genome analysis revealed that la2007 carries a plasmid highly similar to bacillus anthracis pxo1, including the genes responsible for the production and regulation of anthrax toxin. | 2017 | 28428293 |
| complete genome sequence of the nematicidal bacillus thuringiensis mybt18246. | 10.1601/nm.5000 is a rod-shaped facultative anaerobic spore forming bacterium of the genus 10.1601/nm.4857. the defining feature of the species is the ability to produce parasporal crystal inclusion bodies, consisting of δ-endotoxins, encoded by cry-genes. here we present the complete annotated genome sequence of the nematicidal 10.1601/nm.5000 strain mybt18246. the genome comprises one 5,867,749 bp chromosome and 11 plasmids which vary in size from 6330 bp to 150,790 bp. the chromosome contains ... | 2017 | 28852435 |
| levels of l-malate and other low molecular weight metabolites in spores of bacillus species and clostridium difficile. | dormant spores of bacillus species lack atp and nadh and contain notable levels of only a few other common low mol wt energy reserves, including 3-phosphoglyceric acid (3pga), and glutamic acid. recently, bacillus subtilis spores were reported to contain ~ 30 μmol of l-malate/g dry wt, which also could serve as an energy reserve. in present work, l-malate levels were determined in the core of dormant spores of b. subtilis, bacillus cereus, bacillus megaterium and clostridium difficile, using bot ... | 2017 | 28850573 |
| antimicrobial and antioxidant activities of the extract and fractions of tetradenia riparia (hochst.) codd (lamiaceae) leaves from brazil. | tetradenia riparia (lamiaceae) is native to central africa popularly known as myrrh, used in folk medicine to treat various diseases like malaria, gastroenteritis, and tropical skin disease. this research was to evaluate the antioxidant and antibacterial activities of the crude extract (ce) and fractions (fr) of the t. riparia by classical chromatography. the ce of t. riparia leaves was submitted to column chromatographic fractionation to obtain four fractions of the interest, which were identif ... | 2017 | 28840299 |
| biological activity of the non-microbial fraction of kefir: antagonism against intestinal pathogens. | kefir is a fermented milk obtained by the activity of kefir grains which are composed of lactic and acetic acid bacteria, and yeasts. many beneficial health effects have been associated with kefir consumption such as stimulation of the immune system and inhibition of pathogenic microorganisms. the biological activity of kefir may be attributed to the presence of a complex microbiota as well as the microbial metabolites that are released during fermentation. the aim of this work was to characteri ... | 2017 | 28831964 |
| discovery of a potent inhibitor class with high selectivity toward clostridial collagenases. | secreted virulence factors like bacterial collagenases are conceptually attractive targets for fighting microbial infections. however, previous attempts to develop potent compounds against these metalloproteases failed to achieve selectivity against human matrix metalloproteinases (mmps). using a surface plasmon resonance-based screening complemented with enzyme inhibition assays, we discovered an n-aryl mercaptoacetamide-based inhibitor scaffold that showed sub-micromolar affinities toward coll ... | 2017 | 28820255 |
| synergistic antibacterial and antibiofilm efficacy of nisin in combination with p-coumaric acid against foodborne bacteria bacillus cereus and salmonella typhimurium. | the aim of the study was to evaluate possible antibacterial and antibiofilm efficacy of a bacteriocin, nisin with two essential oil components linalool and p-coumaric acid in combination against foodborne bacteria bacillus cereus and salmonella typhimurium. their inhibition effects on planktonic cells and pre-formed biofilms were evaluated using microbroth dilution and checkerboard titration methods. nisin/ p-coumaric acid combination showed synergistic effects against planktonic cells of both t ... | 2017 | 28815637 |
| simultaneous removal of nitrogen and phosphorous from domestic wastewater using bacillus cereus gs-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal. | a newly isolated gs-5 strain exhibiting heterotrophic nitrification, aerobic denitrification and denitrifying phosphorous removal was identified as bacillus cereus gs-5 based on its phenotypic and phylogenetic characteristics. the isolate had exhibited efficient nh4(+)-n, no3(-)-n, no2(-)-n and po4(3-)-p removal from nutrient spiked real domestic wastewater with average rates of 2.62, 2.69, 1.16 and 0.42mgl(-1)h(-1), respectively under aerobic condition. metabolic inhibitor based mass balance an ... | 2017 | 28803098 |
| growth of amanita caesarea in the presence of pseudomonas fluorescens and bacillus cereus. | the ectomycorrhizal (ecm) fungus amanita caesarea cect 20127 was tested in vitro with two potentially mycorrhizal-promoting bacterial strains, pseudomonas fluorescens cect 844 and bacillus cereus cect 148. although p. fluorescens showed spatial and temporal compatibility with a. caesarea, it did not affect growth of the fungus. conversely, b. cereus exhibited no such compatibility and also inhibited fungal growth. the expression pattern of the a. caesarea gene acmst-1 was analysed using real-tim ... | 2017 | 28800852 |
| methionine residues in exoproteins and their recycling by methionine sulfoxide reductase ab serve as an antioxidant strategy in bacillus cereus. | during aerobic respiratory growth, bacillus cereus is exposed to continuously reactive oxidant, produced by partially reduced forms of molecular oxygen, known as reactive oxygen species (ros). the sulfur-containing amino acid, methionine (met), is particularly susceptible to ros. the major oxidation products, methionine sulfoxides, can be readily repaired by methionine sulfoxide reductases, which reduce methionine sulfoxides [met(o)] back to methionine. here, we show that methionine sulfoxide re ... | 2017 | 28798727 |
| potassium-ion-selective fluorescent sensors to detect cereulide, the emetic toxin of b. cereus, in food samples and hela cells. | we report the development of new chemical probes for cereulide, a toxic metabolite produced by specific strains of bacillus cereus, through displacement of potassium cations from a preformed specific complex and a subsequent change in the fluorescence emission. for this purpose, we designed fluorescent probes for potassium cations that were suitable for displacement assays with cereulide from organic extracts. the fluorescence detection of natural cereulide in rice samples was achieved by using ... | 2017 | 28794952 |
| proposal of nine novel species of the bacillus cereus group. | nine novel gram-stain-positive bacteria were investigated by a polyphasic taxonomic approach. based on the analysis of 16s rrna gene sequences, these strains belonged to the bacillus cereus group, sharing over 97 % similarity with the known species of this group, and less than 95 % similarity with other species of the genus bacillus. multilocus sequence typing analysis showed that they formed nine robust and well-separated branches from the known species. the digital dna-dna hybridization (dddh) ... | 2017 | 28792367 |
| novel motb as a potential predictive tool for identification of b. cereus, b. thuringiensis and differentiation from other bacillus species by triplex real-time pcr. | quantitative triplex real-time pcr (qpcr) offers an alternative method for detection of bacterial contamination. it provides quantitation of the number of gene copies. in our study, we established a qpcr assay to detect and quantify the specificity towards bacillus cereus and b. thuringiensis. the assay was designed to detect a 280 bp fragment of motb gene encoding the flagellar motor protein, specific for detection of b. cereus and b. thuringiensis, excluding other group species b. pseudomycoid ... | 2017 | 28778821 |
| gene expression and in silico analysis of snakehead murrel interleukin 8 and antimicrobial activity of c-terminal derived peptide ws12. | chemokines have been known for their wide range of functions including chemoattractant property in humans and other vertebrate organisms. they act as a bridge between innate and adaptive immune system. in the present study, we have identified a cxc chemokine from the cdna library of c. striatus; on the basis of orthology study, it was found highly identical to interleukin 8 (il8). the bioinformatics analysis of the chemokine revealed the presence of a typical γ-core domain and a cxc motif at the ... | 2017 | 28778316 |
| antimicrobial activity of endophytic bacterial populations isolated from medical plants of iran. | endophytic actinobacteria colonize inside the plant tissues without causing damages to the host plant. since these microorganisms colonize in the different parts of plants and can stop plant disease, they have been applied as biological agents for controlling human diseases. the aim of this study was molecular identification and measuring the antimicrobial activity of endophytic actinomycetes isolated from medicinal plants of iran. | 2017 | 28775818 |
| bacillus cereus group bacteriophage flapjack genome sequence. | the bacillus cereus group bacteriophage flapjack, a double-stranded dna (dsdna) myoviridae isolate collected from soil collected in washington, dc, is a member of cluster c3 and encodes an intramolecular chaperone-containing tail fiber protein previously found in podoviridae and siphoviridae but not annotated in the myoviridae. | 2017 | 28774974 |
| pan-genome and phylogeny of bacillus cereus sensu lato. | bacillus cereus sensu lato (s. l.) is an ecologically diverse bacterial group of medical and agricultural significance. in this study, i use publicly available genomes and novel bioinformatic workflows to characterize the b. cereus s. l. pan-genome and perform the largest phylogenetic and population genetic analyses of this group to date in terms of the number of genes and taxa included. with these fundamental data in hand, i identify genes associated with particular phenotypic traits (i.e., "pa ... | 2017 | 28768476 |
| phenolic acids and flavonoids of peanut by-products: antioxidant capacity and antimicrobial effects. | peanut skin (ps) and meal from dry-blanched peanuts (mdbp) were evaluated as sources of phenolic compounds. ps rendered the highest total phenolic content, antioxidant capacity towards abts radical cation, dpph and hydroxyl radicals as well as reducing power. phenolic acids were present in ps and mdbp whereas proanthocyanidins and monomeric flavonoids were found only in ps as identified by hplc-dad-esi-ms(n). procyanidin-rich extracts prevented oxidation in non-irradiated and gamma-irradiated fi ... | 2017 | 28764032 |
| purification and characterization of trypsin produced by gut bacteria from anticarsia gemmatalis. | purification of active trypsin in the digestive process of insects is essential for the development of potent protease inhibitors (pis) as an emerging pest control technology and research into insect adaptations to dietary pis. an important aspect is the presence of proteolytic microorganisms, which contribute to host nutrition. here, we purified trypsins produced by bacteria bacillus cereus, enterococcus mundtii, enterococcus gallinarum, and staphylococcus xylosus isolated from the midgut of an ... | 2017 | 28762531 |
| formulation of menthol-loaded nanostructured lipid carriers to enhance its antimicrobial activity for food preservation. | purpose: due to the antimicrobial property, menthol have significant potential for food preservation and foodstuff shelf life improvement. nevertheless, menthol instability, insolubility, and rapid crystallization in aqueous media make it unsuitable for used in food products. this work was aimed to prepare menthol-loaded nanostructured lipid carriers (nlcs) to enhance its antimicrobial activity. methods: morphology, particle size and size distribution, encapsulation efficiency percent (ee%), and ... | 2017 | 28761828 |
| multiplex pcr coupled with propidium monoazide for the detection of viable cronobacter sakazakii, bacillus cereus, and salmonella spp. in milk and milk products. | cronobacter sakazakii, bacillus cereus, and salmonella spp. are common food-borne pathogens. the aim of this study was to develop a sensitive, specific, and rapid method for the simultaneous detection of these 3 pathogens in milk and milk products. three specific primers were designed based on ompa, inva, and cesb of c. sakazakii, salmonella spp. and b. cereus, respectively, for use in a multiplex pcr (mpcr). to eliminate false-positive results, cells were pretreated with propidium monoazide (pm ... | 2017 | 28755939 |
| cyclohexene-fused 1,3-oxazines with selective antibacterial and antiparasitic action and low cytotoxic effects. | oxazine derivatives, a class of heterocyclic compounds, exhibit a variety of biological properties, such as anticonvulsant and antitumor activities. in this study, we evaluated the effect of two cyclohexene-fused 1,3-oxazines (cis‑1-benzyl-n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (1) and trans‑n-phenyl-1,4,4a,5,8,8a-hexahydro-3,1-benzoxazin-2-imine (2)) in cultures of bacillus cereus, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, salmonella enterica, serratia mar ... | 2017 | 28755871 |
| quantitative prevalence, phenotypic and genotypic characteristics of bacillus cereus isolated from retail infant foods in china. | bacillus cereus is an important foodborne pathogen, which can cause severe food poisoning. the aim of this study was (i) to evaluate the quantitative prevalence of b. cereus in retail prepackaged infant formula and ready-to-eat rice flour in china and (ii) to gain the basic information on pheno- and genotypic characteristics of b. cereus isolates. we found that 40 out of the 587 samples were positive for b. cereus. b. cereus in 3.5% of infant formula samples and 1.0% of rice flour samples outnum ... | 2017 | 28753035 |
| bam35 tectivirus intraviral interaction map unveils new function and localization of phage orfan proteins. | tectiviridae comprises a group of tail-less, icosahedral, membrane-containing bacteriophages that can be divided into two groups by their hosts, either gram-negative or gram-positive bacteria. while the first group is composed of prd1 and nearly identical well characterized lytic viruses, the second one includes more variable temperate phages, like gil16 or bam35, whose hosts are bacillus cereus and related gram-positive bacteria.in the genome of bam35, nearly half of the 32 annotated open readi ... | 2017 | 28747494 |
| antibacterial activity of fusion from biosynthesized acidocin/silver nanoparticles and its application for eggshell decontamination. | bacteriocins from lactic acid bacteria (lab) are useful to control the persistent development of pathogenic microorganisms in food and medicine fields. the bacteriocin acidocin was extracted from lactobacillus acidophilus m1 that was isolated from fermented milk, purified using ammonium sulphate fractionation, and gel filtration column chromatography using sephadex matrix and applied as a potential antibacterial agent. the molecular weight of the purified acidocin was estimated using sds-page to ... | 2017 | 28736866 |
| crystal structures of an atypical aldehyde dehydrogenase having bidirectional oxidizing and reducing activities. | aldehyde dehydrogenases (aldhs) are nad(p)(+)-dependent oxidoreductases that catalyze the oxidation of a variety of aldehydes to their acid forms. in this study, we determined the crystal structures of aldh from bacillus cereus (bcaldh), alone, and in complex with nad(+) and nadp(+). this enzyme can oxidize all-trans-retinal to all-trans-retinoic acid using either nad(+) or nadp(+) with equal efficiency, and atypically, as a minor activity, can reduce all-trans-retinal to all-trans-retinol using ... | 2017 | 28732729 |
| protease hydrolysates of filefish (thamnaconus modestus) byproducts effectively inhibit foodborne pathogens. | in this study, novel antimicrobial peptides had been derived by enzymatic hydrolysis of filefish (thamnaconus modestus) byproduct (hfbp). different proteases, (papain [p], trypsin [t], neutrase [n], pepsin [pe], and the mixture i [pn] and mixture ii [pt]) and different hydrolysis time (60, 120, 180, and 240 min), have been used to generate peptides with different lengths and amino acid sequences. the antimicrobial properties of hfbps were tested, against gram-positive and gram-negative strains, ... | 2017 | 28731389 |
| complete genome sequences of three phi29-like bacillus cereus group podoviridae. | three double-stranded dna phi29-like bacillus cereus group bacteriophages, beachbum, harambe, and serpounce, were identified and characterized. beachbum and harambe are closely related but are remarkably different from previously identified phi29-like phages. serpounce is substantially closer to other phi29-like phages, enabling the identification of its prohead rna (prna) gene. | 2017 | 28729280 |
| an excited state underlies gene regulation of a transcriptional riboswitch. | riboswitches control gene expression through ligand-dependent structural rearrangements of the sensing aptamer domain. however, we found that the bacillus cereus fluoride riboswitch aptamer adopts identical tertiary structures in solution with and without ligand. using chemical-exchange saturation transfer (cest) nmr spectroscopy, we revealed that the structured ligand-free aptamer transiently accesses a low-populated (∼1%) and short-lived (∼3 ms) excited conformational state that unravels a con ... | 2017 | 28719589 |
| sinonasal t2r-mediated nitric oxide production in response to bacillus cereus. | upper airway epithelial cells produce bactericidal nitric oxide (no) in response to both gram-positive and gram-negative bacteria. our previous work demonstrated that t2r38, a bitter taste receptor (t2r) expressed in airway epithelium, produces no in response to quorum-sensing molecules secreted by pseudomonas aeruginosa. we also demonstrated that staphylococci products elicit an no response when using a t2r-independent pathway. when screening additional human pathogens for epithelial t2r activa ... | 2017 | 28716170 |
| characterization of enterotoxigenic bacillus cereus sensu lato and staphylococcus aureus isolates and associated enterotoxin production dynamics in milk or meat-based broth. | bacillus cereus sensu lato species, as well as staphylococcus aureus, are important pathogenic bacteria which can cause foodborne illness through the production of enterotoxins. this study characterised enterotoxin genes of these species and examined growth and enterotoxin production dynamics of isolates when grown in milk or meat-based broth. all b. cereus s. l. isolates harboured nhea, hbla and entfm toxin genes, with lower prevalence of bcet and hlyii. when grown at 16 °c, toxin production by ... | 2017 | 28714887 |
| detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization. | simultaneous and rapid detection of multiple foodborne bacterial pathogens is important for the prevention of foodborne illnesses. | 2017 | 28713514 |
| effect of dairy product environment on the growth of bacillus cereus. | ph is one of the most important parameters to manage bacterial replication in foodstuffs. in this study, the ability of 2 bacillus cereus strains, 1 clinical human isolate (gpe2) and 1 isolate from a dairy product (d43), were investigated for in vitro growth at different ph values (from 3.5 to 7.5) at 2 temperatures (15 and 37°c), showing their ability to grow from 5.5 to 7.5 and from 5.0 to 7.5, respectively. the ability of spores of these 2 microorganisms to germinate in different typologies o ... | 2017 | 28711259 |
| the potential multiple mechanisms and microbial communities in simultaneous nitrification and denitrification process treating high carbon and nitrogen concentration saline wastewater. | a simultaneous nitrification and denitrification (snd) process in sequencing batch biofilm reactor (sbbr) was established to treat high carbon and nitrogen saline wastewater in this study. acetate, glucose and an organic mixture were applied as organic sources in three sbbrs, achieving average total nitrogen removal efficiency of 97.15%, 63.94% and 94.99% during 120days' operation, respectively. the underlying nitrogen removal mechanisms were investigated by 16s rrna sequencing and batch tests. ... | 2017 | 28710998 |
| evaluation of nutrients removal (no3-n, nh3-n and po4-p) with chlorella vulgaris, pseudomonas putida, bacillus cereus and a consortium of these microorganisms in the treatment of wastewater effluents. | in this research removal of nh3-n, no3-n and po4-p nutrients from municipal wastewater was studied, using chlorella vulgaris, pseudomonas putida, bacillus cereus and an artificial consortium of them. the objective is to analyze the performance of these microorganisms and their consortium, which has not been previously studied for nutrient removal in municipal wastewater. a model wastewater was prepared simulating the physicochemical characteristics found at the wastewater plant in chapala, mexic ... | 2017 | 28708609 |
| dynamics of culturable mesophilic bacterial communities of three fresh herbs and their production environment. | investigate dynamics of culturable mesophilic bacteria and selected food-contaminating bacteria from three herbs and their production environment. | 2017 | 28708321 |