Publications

[herpesvirus and acute peripheral facial paresis].		1989	2756590
assignment of human genes for phosphorylase kinase subunits alpha (phka) to xq12-q13 and beta (phkb) to 16q12-q13.	phosphorylase kinase (phk), the enzyme that activates glycogen phosphorylases in muscle, liver, and other tissues, is composed of four different subunits. recently isolated rabbit muscle cdnas for the larger two subunits, alpha and beta, have been used to map the location of their cognate sequences on human chromosomes. southern blot analysis of rodent x human somatic cell hybrid panels, as well as in situ chromosomal hybridization, have provided evidence of single sites for both genes. the alph ...	1989	2757032
time course of the anticonvulsant action of clonazepam during ontogenesis in the rat.	a time course of the protective action of clonazepam against metrazol-induced seizures was studied in 612 rats aged 7, 12, 18, 25 and 90 days. two doses of clonazepam (0.1 and/or 1 mg/kg) were used and metrazol (100 mg/kg in all but 18-day-old animals where 90 mg/kg were given) was administered s.c. from 30 min to 48 hr. not all time intervals were examined in all age groups. eight to 14 rats were used for each time interval, dose and age group. both doses exhibited a clear-cut antimetrazol acti ...	1989	2757463
perioral anesthesia disrupts maternal behavior during early lactation in long-evans rats.	the role of perioral somatosensory stimulation from pups in maternal behavior was investigated in lactating long-evans rats (rattus norvegicus) during early lactation by local anesthesia of the mystacial pads with lidocaine, which blocks conduction in the infraorbital nerve. it was concluded that (1) acute perioral anesthesia markedly inhibited pup retrieval when the subjects had no formal pretreatment retrieval experience. further, these dams displayed little tolerance to the lidocaine treatmen ...	1989	2757582
reduction of doxorubicin toxicity by methylene blue in cultured rat myocardial cells.		1989	2757653
attempted passive transfer of a serum factor in chronic urticaria from human to monkey.		1989	2757947
age changes in the crystallinity of bone mineral and in the disorder of its crystal.	the crystallinity of bone mineral and disorder of the crystal at different ages were measured by the x-ray diffraction method of ruland. measurements were made on femoral mid-diaphyses of wistar rat from 2 weeks to 1 year of age. for a given animal age, the crystallinity of bone mineral increases with age, while the overall disorder of the crystal does not vary within experimental accuracy. the increase in the crystallinity with age is attributed to an increase in crystallite size, a decrease in ...	1989	2758062
characterization of ion movements and their relationship to muscarinic receptor binding and excitation-contraction coupling in guinea pig ileal longitudinal smooth muscle.	the relationship between ion movements (sodium uptake and potassium release) and agonist-induced contractile responses or muscarinic receptor binding was investigated in the guinea pig ileal longitudinal muscle (gplm). sodium uptake and potassium release were agonist-dependent, concentration-dependent, and stereoselective, with the following rank order of maximum ion movement: muscarinic agonists greater than histamine greater than substance p = serotonin. potassium depolarization did not initia ...	1989	2758375
characterization of ion movements and their relationship to muscarinic receptor binding and excitation-contraction coupling in guinea pig ileal longitudinal smooth muscle.	the relationship between ion movements (sodium uptake and potassium release) and agonist-induced contractile responses or muscarinic receptor binding was investigated in the guinea pig ileal longitudinal muscle (gplm). sodium uptake and potassium release were agonist-dependent, concentration-dependent, and stereoselective, with the following rank order of maximum ion movement: muscarinic agonists greater than histamine greater than substance p = serotonin. potassium depolarization did not initia ...	1989	2758375
biological and immunological properties of sugi basic protein-pullulan conjugate. i. suppressive effect on ige antibody production and on ige-mediated reactions.	sugi basic protein (sbp), a major allergen of japanese cedar pollen, conjugated to pullulan acquires suppressive activities for ige, but not for igg antibody production, and for ige-mediated pk reactions in a sbp-specific manner. when either normal or ige anti-sbp antibody-producing mice were treated with the conjugate, ige anti-sbp antibody levels scarcely increased even after immunization with sbp + alum. this suppression was shown to be sbp specific and ige selective. the ability of sbp-pullu ...	1989	2759711
denervation of primate extraocular muscle. a unique pattern of structural alterations.	extraocular muscles differ from most other skeletal muscles in terms of constituent fiber types and innervation pattern. the rules that govern fiber responses to various experimental interventions for most skeletal muscles, therefore, may not strictly apply to the extraocular muscles. in this study, denervation of the extraocular muscles of cynomolgous monkeys, macaca fascicularis, was accomplished by intracranial transection of the oculomotor nerve. survival times of 3-167 days were allowed, an ...	1989	2759804
protection against infection with vibrio cholerae by passive transfer of monoclonal antibodies to outer membrane antigens.	ten monoclonal antibodies (mabs), produced by hybridomas obtained through the cell fusion of mouse myeloma cells and spleen cells from mice immunized with outer membrane antigens of vibrio cholerae, were mixed with suspensions of v. cholerae and administered orally to infant rabbits. six of these mabs, each one recognizing a different outer membrane antigen, did not confer protection from fatal v. cholerae infection. four mabs that reacted with the 18-kda antigen, also called the cholera protect ...	1989	2760481
clinical concentrations of volatile anesthetics reduce depolarization-evoked release of [3h]norepinephrine, but not [3h]acetylcholine, from rat cerebral cortex.	we compared the potencies of halothane, enflurane, and methoxyflurane in producing unconsciousness in vivo and in inhibiting the release of [3h]norepinephrine and [3h]acetylcholine in vitro. rats were anesthetized with various concentrations of each anesthetic, and responsiveness was determined by a hemostat tail pinch. slices of cerebral cortex were equilibrated with similar concentrations of each agent in vitro, and potassium-evoked release of [3h]norepinephrine and [3h]acetylcholine was deter ...	1989	2760627
relationship between concentration and anticonvulsant effect of phenytoin against electroshock-induced seizures in rats: comparison of sampling sites for concentration determinations.	the purpose of this investigation was to determine the optimum sampling site for phenytoin concentration measurements in the context of pharmacodynamic studies of the anticonvulsant effect of phenytoin. determination of drug concentrations in the serum, serum water, brain, and cerebrospinal fluid (csf) of rats as a function of time after iv injection of a 6-mg/kg dose revealed a significant disequilibrium between brain and serum water for 15 min and between csf and serum water for 5 min after in ...	1989	2760817
2-bromo- and 16-bromo-estrogens and related compounds: potential inhibitors for both estradiol 2- and 16 alpha-hydroxylases in rat liver microsomes.	kinetic studies of inhibition of estradiol 2- and 16 alpha-hydroxylase activities in male rat liver microsomes with 2-bromoestrogens, 4-bromo-estrone (4-bre1), 16 alpha- and 16 beta-bromoestrones and 16 beta-acetylthioestrone (16-acse1) were carried out. 2-bromoestrogens and 4-bre1 nonspecifically blocked the two enzyme activities in a competitive manner, and 2-bromo-estradiol (2-bre2) was the most potent inhibitor for the two hydroxylases among the 2- and 4-bromo steroids. kinetic data, the app ...	1989	2761259
tamoxifen decreases the estradiol induced progesterone receptors by interfering with nuclear estrogen receptor accumulation.	the retention time of the estrogen receptor in the nucleus of target cells after antiestrogen treatment has been shown to be longer than after estradiol. this paper describes the accumulation of nuclear estrogen receptors and the obtention of estrogenic responses (i.e. synthesis of cytosolic progesterone receptors and dna) in the rat uterus after tamoxifen treatment in the presence or absence of estradiol. one-week ovariectomized adult rats were implanted with a silicone elastomer capsule contai ...	1989	2761261
influence of alanylglutamine infusion on gastrointestinal glutamine and alanine metabolism in anesthetized dogs.	in this study we administered an infusion of alanylglutamine (10 mumol/kg.min) for 60 minutes to postoperative anaesthetized dogs with catheters placed in the portal and hepatic veins. arterial plasma levels of alanylglutamine were 83 +/- 27 mumol/l 20 minutes after the onset of the infusion and remained constant throughout the infusion period. plasma levels of glutamine and alanine approximately doubled compared with preinfusion levels, indicating a release of these amino acids from the dipepti ...	1989	2761423
spectroscopic imaging by dephasing amplitude changing (sidac).	a technique for spectroscopic imaging based on a new concept of dual encoding (i.e., both phase and frequency encoding) of position along the direction of the read-out gradient is proposed. the phase encoding is produced by changing the amplitude of the dephasing gradient as in conventional phase encoding. high-resolution spectroscopic images of a normal human thigh obtained by a 2.0 tesla whole body mr imaging system are presented.	1989	2762582
[infection caused by paracoccidioides brasiliensis in people living in cocle and veraguas, republic of panamá].	intradermal application of yeast phase paracoccidioidin antigen obtained by sonication and lysis from the fungus to 111 residents of veraguas province (68 men and 43 women) and 130 cocle province residents (57 men and 73 women) elicited a positive reaction in 9.01% (1/111) and 26.15% (34/30) of the veraguas and cocle residents respectively. positive reactions ranged between papules of 5 and 27 mm in diameter, the majority being between 11 and 20 mm. infection was more frequent in men (1:1.47 for ...	1989	2762594
experimental atherosclerosis and oxygen free radicals.	oxygen free radicals are known to produce cellular injury by peroxidation of phospholipids in the cell membrane. these free radicals might damage the endothelial cell and thus set the stage for atherosclerosis. the authors studied the effect of high-cholesterol diets on the genesis of atherosclerosis and lipid peroxidation products, malondialdehyde (mda) in rabbits. the animals were divided into four groups each comprising 5 rabbits, on the basis of their diets. group i, control diet; group ii, ...	1989	2764311
ecology of vibrio cholerae non-o1 and salmonella spp. and role of zooplankton in their seasonal distribution in fukuyama coastal waters, japan.	seasonal variation of human pathogens such as vibrio cholerae non-01 and salmonella spp. in fukuyama coastal waters and the role of zooplankton in their distribution were studies for a period of 1 year. comparison of two established methods, viz., the elevated temperature method and the two-step enrichment method of enumerating v. cholerae, showed that the former is superior in the recoveries of v. cholerae non-01. isolation of this pathogen on a wider range of salinities (0.4 to 32.5%) revealed ...	1989	2764569
effects of co-administration of di(2-ethylhexyl)phthalate and testosterone on several parameters in the testis and pharmacokinetics of its mono-de-esterified metabolite.	the administration of 1 g/kg di(2-ethylhexyl)phthalate (dehp) or 5 mg/kg testosterone for 1 week did not affect the testicular and prostatic gland weights in rats. however, co-administration of dehp and testosterone induced severe testicular atrophy accompanied by a decrease of zinc concentration in the testis and reduction of the activity of testicular specific lactate dehydrogenase isozyme. these changes were similar to the results of high dose administration of dehp alone. values of biologica ...	1989	2764717
effects of antibodies against chicken riboflavin carrier protein on fetal hepatic cell ultrastructure.	day 12 pregnant mice administered antibodies to crcp exhibited riboflavin deprival, which resulted in progressive alteration in the fetal hepatic cell structure, which eventually led to fetal wastage and termination of pregnancy. these changes were evident as early as 1 h following antiserum treatment. three hours following treatment, other degenerative changes such as disorganization of glycogen particles and endoplasmic reticulum and degranulation of mitochondria were observed. nuclear pycnosi ...	1989	2765133
[clinical manifestations of infection by human immunodeficiency virus in children].	hiv infection in children presents a wide spectrum of clinical manifestations, from asymptomatic to opportunistic infections, neurologic deterioration and malignancy. this retrospective study describes the characteristics of 30 hiv infected children, attended at the hospital infantil de méxico. six patients were asymptomatic and 24 symptomatic. twenty four patients presented a secondary infection, 66% opportunistic infections, compared against the 100% who presented around 3 serious bacterial in ...	1989	2765158
[prospects for measuring the rheologic characteristics of human soft tissues based on the recording of their transverse rigidity].	it is shown by an example of a forearm that to determine objective characteristics of rheological properties of soft human tissues by measuring their transverse stiffness (k) one should first investigate the relation between k and the diameter of the used indentor (d). the nonlinearity of the dependence k(d) for indentor diameters from 2 to 8 mm is demonstrated and it is assumed that this nonlinearity is due to heterogeneity of tissue properties in the directions normal to the axis of force acti ...	1989	2765581
hamster circadian rhythms are phase-shifted by electrical stimulation of the geniculo-hypothalamic tract.	the suprachiasmatic nuclei (scn) contain the major pacemaker for mammalian circadian rhythms. the scn receive photic input both directly, via the retinohypothalamic tract (rht), and indirectly, via the geniculohypothalamic tract (ght), which originates in cells in the intergeniculate leaflet (igl) and anterior portions of the ventral lateral geniculate nucleus (vlgn). we tested whether electrical stimulation of the ght would induce phase shifts in wheel-running activity rhythms of syrian hamster ...	1989	2765900
factors affecting the colonization of isolated rabbit intestinal epithelial cells by vibrio cholerae 01 in vitro.	the adhesive capability of vibrio cholerae 01 strains to isolated rabbit intestinal epithelial cells was maximally expressed when the bacteria were grown in synthetic broth and was enhanced by the presence of ca2+ in the growth media. n-acetyl-d-glucosamine could inhibit the adhesion of the bacteria to rabbit intestinal epithelial cells as could lipopolysaccharide o-antigen from vibrio cholerae 01 and lectin from triticum vulgaris. since the lipopolysaccharide is known to contain n-acetyl-d-gluc ...	1989	2766118
purification and partial characterization of a cytotonic enterotoxin produced by aeromonas hydrophila.	this report describes the purification and partial characterization of a cytotonic enterotoxin produced by a human diarrheal isolate (ssu) of aeromonas hydrophila. the extracellular enterotoxin was purified by (nh4)2so4 precipitation, hydrophobic column chromatography, and chromatofocusing. the highly purified enterotoxin exhibited a molecular mass of 44 kda and an isoelectric point in the range of 4.3 - 5.5 as determined by chromatofocusing. western blot analysis using aeromonas anti-enterotoxi ...	1989	2766121
treatment of childhood bacterial meningitis with ceftriaxone once daily: open, prospective, randomized, comparative study of short-course versus standard-length therapy.	fifty-two children were included in this study to evaluate and compare short- versus standard-length ceftriaxone therapy for bacterial meningitis. the duration of the short-course regimens was 4, 6 and 7 days for neisseria meningitidis, hemophilus influenzae and streptococcus pneumoniae, respectively. the standard-length regimens were twice as long. on the basis of a computer-generated randomization list, 26 children were assigned either to the short- or to the standard-treatment regimen. ceftri ...	1989	2766869
transcriptional start site in the mouse cyp1a1 (cytochrome p1450) gene.	two transcriptional initiation sites have been described by different laboratories for the mouse cyp1a1 (cytochrome p1450) gene: start site ti, at the beginning of the nontranslated 87-bp first exon, and start site tii, 635 bp downstream from site ti within the 2,380-bp first intron. site ti was characterized in normal c57bl/6 mouse liver, whereas site tii was described in a high activity variant line (hav cells) derived from the mouse hepatoma hepa-1c1c7 wild type (wt) established cell line. it ...	1989	2766934
exclusion of clinically atypical or microbiologically mixed diarrhoeal episodes from outcome events in a field trial of oral cholera vaccines.	we investigated whether alternative clinical and microbiological criteria for outcome events affected estimates of vaccine efficacy in a randomized, double-blind field trial of b subunit-killed whole cell (bs-wc) and killed whole cell-only (wc) oral cholera vaccines among 62,285 rural bangladeshi participants. at one year of follow-up estimates of vaccine protective efficacy (pe = 60%, p less than 0.0001 for bs-wc; pe = 54%, p less than 0.0001 for wc) against all treated diarrhoeal episodes asso ...	1989	2767860
detection of trypsin- and chymotrypsin-like proteases using p-nitroanilide substrates after sodium dodecyl sulphate polyacrylamide gel electrophoresis.	specific chromogenic p-nitroanilide substrates have proved useful for localizing proteolytic enzymes, such as trypsin, chymotrypsin and elastase after separation by agarose gel electrophoresis and when immobilized on nitrocellulose. this procedure was further developed for use with sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). after sds-page, proteins were transferred electrophoretically to a nitrocellulose membrane. the membrane was incubated for 10-60 min with bz-ile-g ...	1989	2768384
vibrio cholerae non-serogroup o1 cystitis.	we report a case of a patient who developed cystitis caused by non-serogroup o1 vibrio cholerae after swimming in the chesapeake bay. treatment was empirical, with complete symptomatic resolution. genitourinary tract infections by vibrio spp. are uncommon but should be considered when cystitis occurs after saltwater exposure in appropriate geographic regions.	1989	2768474
isolation and morphological characterization of el tor cholera phages.	five bacteriophages have been isolated for phage typing of vibrio cholerae biotype el tor. the morphology of these phages has been studied by electron microscopy.	1989	2769238
studies in fetal wound healing: ii. a fetal environment accelerates fibroblast migration in vitro.	we have used an in vitro model of wound healing using scratches made in a confluent monolayer of fibroblasts. the effects of fetal calf and postnatal calf serum on the migration of fibroblasts were compared. differences between fetal and calf serum-incubated fibroblasts grown on coverslips were observed within 15 minutes of exposure. cells in fetal serum began to change both shape and orientation and to move into the trough created by the scratch. the fibroblasts incubated in fetal calf serum co ...	1989	2769548
studies in fetal wound healing: ii. a fetal environment accelerates fibroblast migration in vitro.	we have used an in vitro model of wound healing using scratches made in a confluent monolayer of fibroblasts. the effects of fetal calf and postnatal calf serum on the migration of fibroblasts were compared. differences between fetal and calf serum-incubated fibroblasts grown on coverslips were observed within 15 minutes of exposure. cells in fetal serum began to change both shape and orientation and to move into the trough created by the scratch. the fibroblasts incubated in fetal calf serum co ...	1989	2769548
[cardiovascular risk factors in children and adolescents in fuenlabrada: objectives, features of the population, design, participation, equipment, laboratory technics, and quality control].	fuenlabrada is at the present time the most industrialized town placed at the south are of madrid. this population has some peculiar attractive characteristics from the epidemiologic point of view. perhaps the most important is that the majority of its inhabitants are immigrant from other depressed rural areas of the country, with a low socio-economic and cultural level. the new conditions of life could have changed the life expectation and the causes of morbidity and mortality of this populatio ...	1989	2772364
infected false aneurysms of the femoral artery in intravenous drug addicts.	the evaluation and treatment of 60 intravenous drug addicts with infected false aneurysms of the femoral artery seen at henry ford hospital (detroit) from 1977 to 1988 were retrospectively reviewed. the most common presenting signs and symptoms were groin swelling and/or mass in 56 (93%) of the patients and pain and/or tenderness in 48 (80%) of the patients. digital subtraction angiography and standard arteriography showed sensitivities of 92% and 96%, respectively, in the detection of aneurysms ...	1989	2772464
pseudomonas cepacia typing systems: collaborative study to assess their potential in epidemiologic investigations.	to determine the utility of available pseudomonas cepacia typing systems for confirming the relatedness of isolates, we applied these methods to isolates associated with previously investigated nosocomial outbreaks. we compared chromosome analysis, serologic reactions, biochemical tests, bacteriocin production and susceptibility, and antimicrobial susceptibility in their ability to determine outbreak relatedness. chromosome analysis, serologic reactions, and biochemical tests were each demonstra ...	1989	2772466
twenty-year comparison of the human allograft and porcine xenograft.	this study compares a retrospective consecutive series of human allografts and concurrent porcine xenografts implanted over a 10-year interval. there were 571 allograft valves and 1,351 xenograft valves implanted in the aortic and mitral position with mean follow-up of 12.8 years for the allografts and 6.2 years for the xenografts. this study compares the incidence of structural deterioration over long-term follow-up. we found that there was no significant difference between four manufacturers o ...	1989	2774760
cholera in louisiana. widening spectrum of seafood vehicles.	the largest cholera outbreak in the united states in over a century occurred in louisiana from august through october 1986. eighteen persons in 12 family clusters had stool culture or serologic evidence of infection with toxigenic vibrio cholerae 0-group 1. thirteen of these persons had severe diarrhea, and 4 required intensive care unit treatment. although all 18 survived, 1 96-year-old woman with suspected cholera died shortly after hospital admission. a case-control study showed that case-pat ...	1989	2774784
primary structure of a novel 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid (sits)-binding membrane protein highly expressed in torpedo californica electroplax.	polyclonal rabbit antibodies were raised against 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid (sits), an inhibitor of a variety of anion transport proteins. these antibodies specifically recognize sits-reacted erythrocyte band 3 in immunoprecipitations and western blots. in western blots of sits-reacted membrane proteins derived from vesicles of the electric organ of torpedo californica (known to express a sits-sensitive cl- channel) the antibodies recognized two major species of ap ...	1989	2775201
metabolism of platelet-activating factor (paf; 1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine) and lyso-paf (1-o-alkyl-2-lyso-sn-glycero-3-phosphocholine) by cultured rat kupffer cells.	the metabolism of platelet-activating factor (paf; identified as agepc: 1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine) and lyso-paf (lyso-gepc: 1-o-alkyl-2-lyso-sn-glycero-3-phosphocholine) was investigated in cultured rat kupffer cells. the rat kupffer cells accumulated [3h]agepc and deacetylated this compound to the corresponding [3h]lyso-gepc, which was the major metabolic product of [3h]agepc. [3h]lyso-gepc was distributed primarily in the supernatant fraction of incubated cells throughout ...	1989	2775221
sialidase from different sources compared for electrophoretically separating serum alkaline phosphatase fractions from liver and bone.	we compared sialidase (neuraminidase; ec 3.2.1.18) from vibrio cholerae, clostridium perfringens, and arthrobacter ureafaciens, seeking to improve the electrophoretic separation of the liver and bone isoenzymes of alkaline phosphatase (ec 3.1.3.1) on cellulose acetate membranes. resolution is decisively determined by the type and activity of sialidase used in the preincubation of serum sample. sialidase from arthrobacter ureafaciens is not suited for this method. for optimal separation of the tw ...	1989	2776324
in vitro determination of phagocytosis and intracellular killing of brucella melitensis by goat's polymorphonuclear phagocytes.	one hundred and forty samples of goats serum taken from animals suspected of having brucellosis, have been studied by trying to assess the phagocytosis in vitro of brucella melitensis rev-1 by polymorphonuclear neutrophil phagocytes opsonized with antibrucella serum. the serum agglutination test and the coombs antiglobulin test were carried out with the serum samples. the opsonization ingestion, intracellular killing and germ killing power of the serum were also assessed. the results demonstrate ...	1989	2776406
comparison of trimethadione and antipyrine as indicators of oxidative drug metabolizing capacity in man.	ten healthy male volunteers were given trimethadione (tmo) 4 mg/kg and antipyrine (ap) 500 mg alone or concomitantly to determine whether the metabolism of the drugs was mediated by the same or closely related forms of cytochrome p-450. whether administered alone or together the clearance (cl) and half-life (t 1/2) of tmo and ap were the same, and there was a good correlation between the cl and t 1/2 of tmo and ap (alone r = 0.755 and 0.623, respectively; coadministered r = 0.771 and 0.503, resp ...	1989	2776822
comparative pharmacokinetics of zidovudine (azt) and its metabolite (g.azt) in healthy subjects and hiv seropositive patients.		1989	2776824
suppression of antibody responses to the acetylcholine receptor by natural antibodies.	some relationships between "natural" physiological autoreactivity and antibody responses against the acetylcholine receptor (achr) were investigated. collections of unselected monoclonal antibodies were derived from nonmanipulated newborn mice of susceptible (balb.b) and resistant (balb/c) mouse strains, and screened for reactivity with heterologous achr. five in 200 balb/c but none in 200 balb.b clones were found reactive. four of these anti-achr antibodies were analyzed for connectivity to nin ...	1989	2776827
comparison of selective and complete inhibitors of enkephalin-degrading enzymes on morphine withdrawal syndrome.	we investigated the effects of thiorphan, a selective inhibitor of endopeptidase 24.11 'enkephalinase', kelatorphan ((r)-3-(n-hydroxy-carboxamido-2-benzylpropanoyl)-l-alanine), and rb 38 a ((r)-3-(n-hydroxycarboxamido-2-benzylpropanoyl)-l-phenylalanine) two almost complete inhibitors of enkephalin metabolism, on the naloxone-precipitated morphine withdrawal syndrome in rats. inhibitors administered intracerebroventricularly reduced several symptoms of the withdrawal syndrome. jumping, chewing an ...	1989	2776828
neutralisation of the new cholera toxin by antiserum against crude enterotoxin of cholera toxin gene-positive vibrio cholerae 01 in rabbit ileal loop model.	the enterotoxicity of the new cholera toxin (nct) prepared from cholera toxin gene-negative (ct-) v. cholerae 01 strains isolated from human diarrhoeal and environmental sources was assayed in rabbit ileal loops and the toxin unit was calculated to be 24 micrograms of protein. the enterotoxicity of the nct preparations were completely neutralised by the antiserum raised against the enterotoxin preparation from the ct+ v. cholerae 01 strain 569b at 1 in 16 dilution in ileal loops. the antiserum c ...	1989	2777342
a survey of the human t-cell lymphotropic virus type i (htlv-i) in south-western colombia.	to evaluate altitude of birthplace and residence as factors associated with geographic clustering of htlv-i infection in colombia, we sampled a total of 670 current residents of the south pacific coastal lowland and of upland regions (cali and environs) of the valle and cauca provinces, located at an altitude of 3,100 ft. among the 255 lowland study subjects, 4.3% had antibody against htlv-i, compared to 0.9% of the 415 upland study subjects. a hypothesis emerging from this study is that the low ...	1989	2777408
an actin-depolymerizing protein in embryonic chicken skeletal muscle: purification and characterization.	in embryonic skeletal muscle, a large amount of non-polymerized actin exists in the cytoplasm (shimizu and obinata [1986] j. biochem. 99, 751-759). a 19-kda protein (called 19k protein) which binds to g-actin was purified by sequential chromatography on dnase i-agarose, hydroxylapatite, sp-sephadex, and sephadex g-75, from the sarcoplasmic fraction of embryonic chicken skeletal muscle. this protein decreased the extent of actin polymerization at a steady state and increased the monomeric actin i ...	1989	2777748
[total anomalous pulmonary venous connection in adult: report of a case].	total anomalous pulmonary venous connection (tapvc) is a cardiac disorder that seldom permits survival into adulthood without surgical correction in infancy. we reported a successful total correction in a 47-year-old man with tapvc. cardiovascular angiography demonstrated the features of darling i a type tapvc. cardiac catheterization showed qp/qs: 5.8, pp/ps: 0.31, rp/rs: 0.06 and mild pulmonary hypertension. on operation, the posterior wall of left atrium anastomosed to the common pulmonary ve ...	1989	2779053
hemolytic vibrio cholerae o1 that is sensitive to mukerjee's cholera phage iv and the phage produced by the hemolytic vibrio lysogenized with the infection of mukerjee's cholera phage iv.	strains of hemolytic vibrio cholerae o1 (el tor vibrio) which are sensitive to mukerjee's cholera phage group iv were isolated from cholera patients in north-east thailand in 1986. plaques of the phage on these hemolytic v. cholerae o1 were usually translucent but almost transparent on some strains, just like the plaques on non-hemolytic v. cholerae o1 (classical vibrio). these hemolytic v. cholerae o1 were lysogenized with the infection of cholera phage iv, and the lysogenized strains produced ...	1989	2779469
sugar composition of the polysaccharide portion of lipopolysaccharides isolated from non-o1 vibrio cholerae o2 to o41, o44, and o68.	the sugar composition of the polysaccharide portion of lipopolysaccharides (lps) was determined for 42 serovars of non-o1 vibrio cholerae, i.e., from serogroups o2 to o41, o44, and o68. on the basis of their compositional sugar pattern, they were classified into 24 chemotypes. 2-keto-3-deoxyoctonate (kdo) was totally undetectable by the conventional color test (weissbach's reaction) under the conventional hydrolysis conditions. instead, a kind of kdo-like substance, which was positive in the rea ...	1989	2779472
purification of cross-reacting protein antigen shared by yersinia enterocolitica and other gram-negative bacteria with monoclonal antibody.	a monoclonal antibody against the yersinia enterocolitica 60-kilodalton (kda) antigen, designated cross-reacting protein antigen (crpa), was obtained by cell fusion. the crpa common to gram-negative bacteria was purified from y. enterocolitica by the affinity chromatography with the monoclonal antibody (igg1) thus obtained. the purified crpa showed a single band of 60 kda in sds-polyacrylamide gel electrophoresis (sds-page), and reacted with rabbit antisera against y. enterocolitica, vibrio chol ...	1989	2779474
evolution of androgen-regulated ornithine decarboxylase expression in mouse kidney.	the analysis of naturally occurring variations in hormone-regulated gene expression generates insights into the mechanisms governing evolutionary changes in hormone response. in the mouse (genus mus), kidney ornithine decarboxylase (odc) expression, which is regulated by androgens, has been modified during evolution. this has resulted in intra- and interspecies variations that have accumulated over a 10-15 million year time period. we have examined odc expression and its response to androgens in ...	1989	2779581
rat gene 33: analysis of its structure, messenger rna and basal promoter activity.	several overlapping cdna and genomic dna clones corresponding to mrna g33 and gene 33, respectively, were isolated and characterized. the mrna g33 molecule is 2971 nt in length, exclusive of a poly(a+) tail, and encodes a putative 459 amino acid protein (49,946 daltons). the 13.2 kbp gene consists of four exons, three introns, and has two initiation sites located 27 and 30 bp downstream from a tata box. transfection of h4iie cells with a fusion gene 33 (-1900 to +32)/luciferase construct (psl330 ...	1989	2780291
reasons for the ineffectiveness of eglin c to ameliorate endotoxin shock in sheep.		1989	2780742
opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony.		1989	2780954
skin test responsiveness to a series of new tuberculins of children living in three vietnamese cities.	a skin test survey was conducted among 1035 children aged 7-19 years living in three cities in vietnam. fifteen new tuberculins, including leprosin-a, were applied; an induration of 2 mm diameter or more was considered positive. compared to some other tropical countries, low levels of sensitisation were recorded and remarkable regional differences were found. positivity to any tuberculins (pooled data) among non-bcg-vaccinated children was significantly lower in hanoi (13.1%) and hochiminh-city ...	1989	2781608
growth of staphylococcus aureus and synthesis of enterotoxins in home-made yoghurt.	staphylococcus aureus strains fri-100, s6, fri-137 and fri 472 were inoculated into milk to study growth and enterotoxin production in home-made yogurts. the yogurt used as starter was progressively weakened by successive inoculations (up to four) in milk to prepare other yogurts in order to study the ability of yogurt microflora to inhibit staphylococci. after elaboration, yogurts were stored at 4 degrees c, 22 degrees c, and 37 degrees c for a maximum of 21 days. periodically, staphylococcal c ...	1989	2781867
[comparative evaluation of the levels of water-soluble glial fibrillary acidic protein in the human brain in the normal state and in schizophrenia].	the concentration of water-soluble glial fibrillary acidic protein was measured in several regions of human brains (12 schizophrenic patients and 13 patients without any signs of mental disease) using elisa technique. the content of this protein was lower in thalamus and gyrus cinguli in schizophrenics as compared to normals (p less than 0.01). the protein showed no correlation with sex, age and the terms of autopsy (5 to 7 h after death). the findings suggest that decreasing concentration of th ...	1989	2781906
quantitative assessment of the biological effects of follicle regulatory protein on dihydrotestosterone-maintained spermatogenesis in hypophysectomized rat.	follicle regulatory protein (frp) isolated from porcine ovarian follicles influences folliculogenesis through a paracrine mechanism. a similar protein has been found in the testes and seems to have some inhibitory effects on spermatogenesis when administered to intact male experimental animals. on the basis of female and male studies, it has been ascertained that the effects of frp are at the level of gonads and not the pituitary or the hypothalamus. in the studies with intact males it was not p ...	1989	2782630
human eosinophils express cd4 protein and bind human immunodeficiency virus 1 gp120.	the cd4 glycoprotein, expressed on leukocytes belonging to subsets of t lymphocytes and to cells of monocyte/macrophage lineage, participates in the functioning of t cells and serves as a receptor for hiv-1 and hiv-2. human eosinophils, a class of granulocytic leukocytes, have been found to express cd4. with anti-cd4 mabs cd4 was demonstrable on eosinophils from both normal and eosinophilic donors. eosinophils synthesized a 55-kd cd4 polypeptide immunoprecipitable with two anti-cd4 mabs. eosinop ...	1989	2783333
bone marrow progenitor cells induce a regulatory autologous proliferative t lymphocyte response.	the proliferative response of human t lymphocytes to autologous bone marrow progenitor cells was studied by in vitro coculture in autologous serum. irradiated enriched bone marrow progenitor cells induced the proliferation of cocultured peripheral blood t cells, with maximal proliferation at 8 days and stimulator:proliferator ratios of 1/1. this autologous proliferative t lymphocyte response was completely abrogated by the inclusion of anti-hla-dr, anti-cd2, or anti lfa-3 antibodies into the coc ...	1989	2783600
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.	moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...	1989	2783611
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.	moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...	1989	2783611
introduction of a ha-ras oncogene into rat liver epithelial cells and parenchymal hepatocytes confers resistance to the growth inhibitory effects of tgf-beta.	growth of rat liver epithelial cells (rlec) and primary cultures of parenchymal hepatocytes is potently inhibited by tgf-beta. transfection of a mutated ha-ras oncogene, but not a human c-myc oncogene, into rlec resulted in cell lines resistant to growth inhibition by tgf-beta under anchorage-dependent conditions. infection of primary rat hepatocyte cultures with v-ha-ras yielded a cell line likewise insensitive to inhibition by tgf-beta. binding of [125i]tgf-beta to ha-ras-transfected rlec was ...	1989	2783773
characteristics of human synovial fibroblast activation by il-1 beta and tnf alpha.	human synovial fibroblast cell lines (hsn), established from tissues obtained from the knee joints of arthritis patients undergoing arthoplasty, were used to investigate the effects of human interleukin-1 (il-1) beta and tumour necrosis factor (tnf)alpha on proliferation and prostaglandin e2 (pge2) secretion. il-1 beta and tnf alpha were equipotent stimulators of hsn proliferation. classical non-steroidal anti-inflammatory drugs and glucocorticoids significantly augmented this effect. in additio ...	1989	2784408
post-irradiation regeneration of early b-lymphocyte precursor cells in mouse bone marrow.	to examine the sequential development of early b-cell precursors in mouse bone marrow, b-lineage cells have been examined during a wave of post-irradiation regeneration. cell phenotypes have been defined using double-immunofluorescence labelling techniques for (i) terminal deoxynucleotidyl transferase (tdt); (ii) b220 glycoprotein, detected by the binding of mab 14.8; (iii); mu heavy chains in the cytoplasm (c mu) and at the cell surface (s mu). three populations of mu- cells (tdt+14.8-; tdt+14. ...	1989	2784779
tumor necrosis factor-alpha activation of the il-2 receptor-alpha gene involves the induction of kappa b-specific dna binding proteins.	tnf-alpha induces the expression of il-2r and promotes the proliferation and differentiation of t and b cells. in this report, we have studied the biochemical basis for tnf-alpha activation of the il-2r alpha (tac, p55) gene. transfection of human t cell lines with selectively mutated forms of the il-2r alpha promoter revealed that a kappa b element (nucleotides -267 to -256), as well as 5' flanking sequences (nucleotides -281 to -271) are required for tnf-alpha induction of this transcriptional ...	1989	2785134
production of human cells expressing individual transferred hla-a,-b,-c genes using an hla-a,-b,-c null human cell line.	we detail in this report the characterization of a human b-lymphoblastoid cell line, .221, that does not express endogenous hla-a, hla-b, or hla-c class i ag due to gamma-ray-induced mutations in the hla complex. mutant .221 is characterized by: 1) complete absence of hla-a,-b,-c mrna transcripts and alpha-chains, and 2) intracellular expression of two non-a,-b,-c class i alpha-chains with an abundance less than or equal to 1% of normal hla-a,-b,-c expression on similar cells. however, transferr ...	1989	2785140
trophic effects of first-trimester human trophoblasts and human chorionic gonadotropin on lymphocyte proliferation.	we examined the effects of pure first-trimester human trophoblast cells grown in long-term cultures or their secreted products on the proliferation of human peripheral blood lymphocytes cultured alone, under allogeneic stimulation, or in the presence of concanavalin a. both trophoblasts and their culture supernatants stimulated lymphocyte proliferation. culture supernatant had a moderate enhancing effect on lymphocyte mitogenesis in mixed lymphocyte cultures and in the presence of concanavalin a ...	1989	2785344
two cases of pneumocystis carinii pneumonia occurred in human immunodeficiency virus infected patients: supplemental treatment with aerosolised pentamidine isethionate.	two cases of pneumocystis carinii pneumonia occurred in human immunodeficiency virus infected patients who were diagnosed by the aspiration-lung-biopsy. they were treated with 4 mg/kg of pentamidine isethionate intravenously administered followed by pentamidine aerosol inhalation. although clinical and laboratory findings were improved by the fifth or seventh day of intravenous therapy, substantial leukocytopenia, from 3,000/cmm to 600/cmm and from 2,700/cmm to 1,000/cmm, occurred. at this point ...	1989	2786100
co-localization of corticotropin-releasing factor- and enkephalin-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas.	the co-localization of corticotropin-releasing factor (crf)- and enkephalin (enk)-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas was investigated by the simultaneous application of immuno-beta-galactosidase staining and the peroxidase-antiperoxidase (pap) method to the same sections. crf-like immunoreactive cells were stained blue with immuno-beta-galactosidase staining and enk-like immunoreactive cells brown with the pap method. double-labeled cells with overl ...	1989	2786446
human t lymphocytes possess a limited in vitro life span.	the t lymphocyte offers certain theoretical advantages over other available cell types for the study of aging. immunosenescence is a well-established part of, and may be directly relevant to, mammalian aging, and the t lymphocyte is well-characterized as to function, cell-surface antigen make-up, and other factors. however, prior efforts at studying in vitro aging of t cells have been hampered by poor reproducibility in doubling potential and the occurrence of a peculiar type of crisis. we have ...	1989	2786475
sv40 t antigen acts as a minor histocompatibility antigen of sv40 t antigen tolerant transgenic mice.	the ability of normal mice to mount an sv40 t antigen-specific cytolytic t lymphocytes response when immunized in vivo with splenocytes from the sv40 t antigen transgenic 427-line mice and restimulated in vitro with sv40-transformed fibroblasts, or when immunized with sv40 and restimulated with 427-line splenocytes, was analyzed. both immunization schemes resulted in an sv40 t antigen-specific immune response, indicating the presence of sv40 t antigen-positive cells in the spleens of these trans ...	1989	2786497
enhanced antitumor effect of combination therapy with interleukin-2 and polyribonucleotides against adenocarcinoma 755.	human recombinant interleukin-2 (il-2) and interferon (ifn) inducers in combination were evaluated for their in vivo antitumor efficacy in relation to s.c.-implanted adenocarcinoma 755 in c57bl/6 mice. two ifn inducers, polyinosinic-polycytidylic acid [poly(i)poly(c)] and polyadenylic-polyuridylic acid [poly(a)poly(u)], induced high ifn production in various tissues for a long time compared to treatment with murine interferon-beta. especially, poly(i)poly(c) at 5 mg/kg, the maximum tolerated dos ...	1989	2786554
cholinergic contraction to field stimulation in coronary arteries of cattle.	epicardial coronary artery strips of cattle hearts mounted in vitro respond to transmural stimulation with a neurogenic constriction, attributable to the endogenous release of acetylcholine. these responses were elicited with frequencies as low as 1.0 hz and with pulse durations as low as 100 microseconds. the magnitude of the contractile response increased with increasing frequency of stimulation. blockade of adrenergic neuronal mechanisms with guanethidine (5 x 10(-6) m) increased the size of ...	1989	2786563
systemic delayed-type hypersensitivity to cholera toxin and a detoxified derivative.	the delayed-type hypersensitivity (dth) response to cholera toxin (ct) and an immunopurified formalinized toxoid (td) was studied in adult balb/c mice. intradermal injection of ct in non-immune animals produced substantial footpad oedema which interfered with the measurement of specific dth but td proved satisfactory as a challenge antigen. dth to ct was induced by doses ranging from 0.1 to 10 micrograms, in complete freund's adjuvant (cfa). the optimal reaction was primed for by 1 microgram ct. ...	1989	2786775
abnormalities in growth characteristics of aortic smooth muscle cells in spontaneously hypertensive rats.	comparative studies have shown that cultured vascular smooth muscle cells from spontaneously hypertensive rats (shr) proliferate to a higher cell number, grow to a greater density, and have greater specific growth rate, particularly at a higher saturation density, than those of the normotensive wistar-kyoto (wky) control rats. the growth difference was not due to varying cell survival nor to attachment ability after passage. the degree of dna synthesis was estimated by [3h]thymidine incorporatio ...	1989	2786847
an alternative cytoplasmic domain of the integrin beta 3 subunit.	a cdna encoding a new form of the shared beta subunit (beta 3) of the platelet integrin gpiib/iiia and the vitronectin receptor was isolated from a placental cdna library by screening with a beta 3 (gpiiia) dna probe. this beta 3 variant differs from the previously reported beta 3 in that the cytoplasmic domain is 8 amino acids shorter and has an alternative, 13-amino acid cooh-terminal peptide. the 3' untranslated region of the cdna also differs from the previously reported sequence, while the ...	1989	2787511
effect of protein kinase c inhibitor (h-7) and calmodulin antagonist (w-7) on pertussis toxin-induced il-1 production by human adherent monocytes. comparison with lipopolysaccharide as a stimulator of il-1 production.	human adherent monocytes stimulated with 1 microgram/ml pertussis toxin (pt) produced interleukin-1 (il-1), as measured by thymocyte co-stimulation assay and enzyme-linked immunosorbent assay (elisa), specific for il-1 alpha and il-1 beta. to clarify the role of protein kinase c (pkc) and calmodulin in il-1 production, we investigated the effects of a pkc inhibitor, h-7, and a calmodulin antagonist, w-7 on pt- and lipopolysaccharide (lps)-induced il-1 production by monocytes. addition of 10 micr ...	1989	2787778
differential exposure of the major gangliosides in rabbit thymocytes to vibrio cholerae neuraminidase.	neuraminidase treatment of lymphocytes is known to cause changes of cellular responses in several biological phenomena, but the molecules modified on the cell surface by neuraminidase are not known in detail. rabbit thymocytes, which contain tissue-characteristic gangliosides, were treated with vibrio cholerae neuraminidase, and the susceptibility of the cell surface sialic acid residues was examined. the amount of sialic acid released from the thymocytes at the highest level was 42.4 nmol per 1 ...	1989	2787793
inhibition of enteropathogenic bacteria by human milk whey in vitro.	diarrhea caused by enteropathogenic bacteria is a leading cause of childhood mortality world-wide, particularly in less developed regions. breast-feeding has been advocated to protect infants and children from infectious illnesses. we examined the antibacterial activity of human whey in vitro against multiple strains of the following species of enteropathogenic bacteria, campylobacter jejuni, escherichia coli, salmonella typhimurium, shigella flexneri, shigella sonnei and vibrio cholerae, all is ...	1989	2787902
effect of doxorubicin on mouse hybridoma b cells: stimulation of immunoglobulin synthesis and secretion.	the purpose of these studies was to investigate whether the cell-differentiating effect of anthracyclines can trigger an over-production and secretion of molecules that may interfere with the tumor-host relationship. we exposed mouse hybridoma b-cells, which are devoted to immunoglobulin production, to doxorubicin (10-40 ng/ml). we found that most doxorubicin-treated cells secreted 3- to 5-fold higher amounts of immunoglobulin than untreated cells, along with an accumulation of 50% of them in th ...	1989	2788496
a t-cell-specific transcriptional enhancer element 3' of c alpha in the human t-cell receptor alpha locus.	a transcriptional enhancer element has been identified 4.5 kilobases 3' of c alpha (constant region alpha chain) in the human t-cell receptor (tcr) alpha-chain locus. this enhancer is active on both a tcr v alpha (variable region alpha chain) promoter and the minimal simian virus 40 promotor in tcr alpha/beta jurkat and el4 cells but is inactive on a v alpha promoter in human tcr gamma/delta peer and molt-13 cells, clone 13 b cells, and hela fibroblasts. the enhancer has been localized to a 116- ...	1989	2788889
effect of vitamin d metabolites on calcitriol metabolism in experimental renal failure.	previous studies from our laboratory have demonstrated that metabolic clearance rate (mcr) of calcitriol is decreased in experimental renal failure. in this experiment, we examined the effects of calcitriol, 25-hydroxyvitamin d3 (25(oh)d3) and 24,25-dihydroxyvitamin d3 (24,25(oh)2d3) on the mcr of calcitriol in renal failure produced in rats by partial nephrectomy. the mcr of calcitriol in these rats with renal failure was significantly lower than in control rats with sham operations. plasma con ...	1989	2789310
potentiation by vestibular stimulation of the immobility reflex elicited by clamping in developing and adult rats.	we have previously described (exp. neurol., 97: 315-326, 1987) that clamping of the neck elicits a profound immobility with flexion of limbs and ventroflexion of head and neck ('carrying posture'). we also observed that when a clamped rat pup is carried by the experimenter's hand, such carrying posture was potentiated. in the present paper we observed that in adult rats vestibular stimulation by circular acceleration potentiates the duration of immobility reflex by clamping and intensifies the l ...	1989	2789702
squamous cell carcinoma of the base of the tongue: results of treatment in 115 cases.	between 1976 and 1986, we treated 115 patients (mean age 53.8 years) with base of tongue carcinomas. the staging system used was the uicc tnm classification of 1979. seventy per cent of the tumours were t3 or t4 and 42% had n2 or n3 lymph node. locoregional treatment was irradiation alone (98/115) or surgery and post-operative radiotherapy (17/115). sixty-seven patients received induction chemotherapy. actuarial survival of the entire group at 3 and 5 years was 25 and 23%, respectively, and 3-ye ...	1989	2790426
[clinical and experimental research on a kidney-tonifying prescription in preventing and treating children's hearing-loss induced by aminoglycoside antibiotic ototoxicity].	this paper reports 30 cases of hearing-loss induced by aminoglycoside antibiotic ototoxicity treated mainly with kidney-tonifying herbs such as herba epimedii, rhizoma drynariae, rhizoma polygonati, radix polygoni multiflori, magnetitum and rhizoma acori graminei, etc. after three month's treatment, the authors found that 19 cases had improved their hearing more than 15 db, among which there are 8 cases who had improved 15 db and 11 cases 30 db. the patients' hearing of pre-treatment and post-tr ...	1989	2791158
relative contribution of various forms of cytochrome p450 to the metabolism of benzo[a]pyrene by human liver microsomes.	a variety of cytochromes p450 have been implicated in the hepatic metabolism of benzo[a]pyrene (bp), including forms that are constitutively expressed and those that are highly inducible. in the present study the metabolism of bp to organic solvent-soluble derivatives by eight forms of cytochrome p450 isolated from rat liver and by a series of 11 human liver microsomal samples was investigated. the relative contribution of specific p450 forms to the human hepatic metabolism was evaluated. a 4-fo ...	1989	2791199
effect of platelet factors on migration of cultured bovine aortic endothelial and smooth muscle cells.	endothelial cell (ec) injury and the response of ec and smooth muscle cells (smcs) to injury contribute to the pathophysiology in patients with vascular disease and atherosclerosis. since platelets have been suggested to play an important role in modulating vascular injury, the present study was undertaken to examine the influence and mechanism of action of individual platelet factors on bovine aortic ec and smc migration using an in vitro wound assay system. serotonin decreased ec proliferation ...	1989	2791219
intensive care for patients with pneumocystis carinii pneumonia and respiratory failure. are we prepared for our new success?		1989	2791661
[ferric ammonium citrate as virulence-enhancing agent in the toxicity test of vibrio cholerae and the potency assay of cholera vaccine].	using icr strain mice as experimental animals, 0.25% (w/v) ferric ammonium citrate (fac) was a suitable substitute for 5% (w/v) mucin as a virulence-enhancing (ve) agent in the toxicity test of vibrio cholerae and the potency assay of cholera vaccine. there was no significant lethal toxicity difference between 0.25% fac and 5% mucin as ve agents on vibrio cholerae in mice. by using 0.25% fac as a ve agent in estimating the potency of cholera vaccine, the relative potency (r.p.) of the tested cho ...	1989	2791724
visual evoked potentials in prader-willi syndrome.	oculocutaneous, electrophysiological, and cytogenetic factors were evaluated in 14 patients with prader-willi syndrome and in three controls, two albinos and a normal observer. in a substantial number of pw patients chromosomal anomalies, particularly deletions of the long arm of chromosome 15 as well as hypopigmentation of hair, skin, and eye have been identified. in the genetic condition of albinism, hypopigmentation related to neural ectoderm derivatives is associated with reduced visual acui ...	1989	2791841
interaction between prostatic fibroblast and epithelial cells in culture: role of androgen.	we have established and characterized two cell lines from the ventral prostate gland of normal nb rats: nbe-1 (prostatic epithelial) and nbf-1 (prostatic fibroblast) cell lines. to identify the direct mitogenic action of dihydrotestosterone (dht), we incubated these cell lines alone and together (in the presence and absence of cell contact) with various concentrations of dht (0.1-10,000 ng/ml) for 24-72 h and assayed for the rate of dna synthesis and the total number of cells in tissue culture a ...	1989	2792005
bolus injections of recombinant human interleukin-1 cause transient hypocalcemia in normal mice.	single daily injections of recombinant human interleukin-1 alpha (il-1 alpha) or interleukin-1 beta (il-1 beta) were given for 1-3 days to normal mice, and blood ionized calcium concentrations were measured before and at various times after each injection. mean blood calcium levels fell significantly in both groups of animals 3 h after the first il-1 injections and returned to pretreatment values at 5 h. however, at 24 and 48 h mean values were significantly higher than those in saline-heated co ...	1989	2792008
mechanisms of arterial thrombosis in nonparallel streamlines: platelet thrombi grow on the apex of stenotic severely injured vessel wall. experimental study in the pig model.	the role of thrombosis in various acute coronary syndromes has been established. however, the basic mechanism by which plaque rupture leads to a growing thrombus in the vicinity of stenotic lesions is not well understood. using a characterized flow chamber in a rheologically controlled system, we have mimicked stenotic vessels and studied for the first time cell-vessel wall interaction in nonparallel streamlines. stenoses ranging from 0 to 80% were produced with stripped tunica media to mimic se ...	1989	2794050