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arsenite oxidizing multiple metal resistant bacteria isolated from industrial effluent: their potential use in wastewater treatment.arsenite oxidizing bacteria, isolated from industrial wastewater, showed high resistance against arsenite (40 mm) and other heavy metals (10 mm pb; 8 mm cd; 6 mm cr; 10 mm cu and 26.6 mm as(5+)). bacterial isolates were characterized, on the basis of morphological, biochemical and 16s rrna ribotyping, as bacillus cereus (1.1s) and acinetobacter junii (1.3s). the optimum temperature and ph for the growth of both strains were found to be 37 °c and 7. both the strains showed maximum growth after 24 ...201627339314
binary interactions of antagonistic bacteria with candida albicans under aerobic and anaerobic conditions.we used both aerobic and anaerobic liquid co-cultures, prepared with luria bertani broth, to study the effect of bacteria on the survival of candida albicans in the external environment, away from an animal host. the bacteria were represented by aeromonas hydrophila, bacillus cereus, bacillus subtilis, clostridium, enterobacter, klebsiella pneumoniae, kluyvera ascorbata and serratia marcescens. under aerobic conditions, the yeast's growth was inhibited in the presence of bacterial growth; howeve ...201626566932
the identification of intrinsic chloramphenicol and tetracycline resistance genes in members of the bacillus cereus group (sensu lato).bacillus toyonensis strain bct-7112(t) (ncimb 14858(t)) has been widely used as an additive in animal nutrition for more than 30 years without reports of adverse toxigenic effects. however, this strain is resistant to chloramphenicol and tetracycline and it is generally considered inadvisable to introduce into the food chain resistance determinants capable of being transferred to other bacterial strains, thereby adding to the pool of such determinants in the gastro-enteric systems of livestock s ...201628101085
physicochemical analyses of a bioactive 4-aminoantipyrine analogue - synthesis, crystal structure, solid state interactions, antibacterial, conformational and docking studies.a novel schiff base derivative of 4-aminoantipyrine, that is, (e)-4-(2-methoxybenzylideneamino)-1,5-dimethyl-2-phenyl-1h-pyrazol-3(2h)-one (mba-dmpp), was synthesized and characterized by ft-ir, (1)h-nmr, and ei-ms. single-crystal x-ray diffraction data revealed mba-dmpp adopts a trans configuration around its central c=n double bond, and forms orthorhombic crystals. xrd revealed that mba-dmpp possess two different planes, in which the pyrazolone and benzylidene groups attached to c9 of the pyra ...201628096791
identification of fatty acids in bacillus cereus.the bacillus species contain branched chain and unsaturated fatty acids (fas) with diverse positions of the methyl branch (iso or anteiso) and of the double bond. changes in fa composition play a crucial role in the adaptation of bacteria to their environment. these modifications entail a change in the ratio of iso versus anteiso branched fas, and in the proportion of unsaturated fas relative to saturated fas, with double bonds created at specific positions. precise identification of the fa prof ...201628060260
risk assessment in the recovery of food for social solidarity purposes: preliminary data.the most recent study, conducted by politecnico of milan, on food surplus management in italy shows that in the italian food supply chain the food surplus is around 5.5 million tons/year, and the amount of food wasted is around 5.1 million tons/year. during 2015, the charitable organizations (cos) belonging to the italian food bank network, active in recovering and distributing food for social solidarity's purposes, reused 381,345 tons of food from 2292 donors. the main supplying sources of the ...201628058252
in vitro evaluation of the antimicrobial ability and cytotoxicity on two melanoma cell lines of a benzylamide derivative of maslinic acid.maslinic acid is a pentacyclic triterpene extracted from olives that has been systematically reported to exert several therapeutic effects, such as antitumoral, antidiabetic, antioxidant, anti-inflammatory, antiparasitic, and antiviral properties. recently, new derivatives of maslinic acid have been obtained and expanded the spectrum of biological activities and improved the existing ones. the present study was meant to perform the in vitro assessment of the (i) cytotoxic effects of a benzylamid ...201628050335
antimicrobial activity of kefir against various food pathogens and spoilage bacteria.kefir is a unique fermented dairy product produced by a mixture of lactic acid bacteria, acetic acid bacteria, and yeast. here, we compared the antimicrobial spectra of four types of kefirs (a, l, m, and s) fermented for 24, 36, 48, or 72 h against eight food-borne pathogens. bacillus cereus, staphylococcus aureus, listeria monocytogenes, enterococcus faecalis, escherichia coli, salmonella enteritidis, pseudomonas aeruginosa, and cronobacter sakazakii were used as test strains, and antibacterial ...201628115890
epidemiology of bacterial toxin-mediated foodborne gastroenteritis outbreaks in australia, 2001 to 2013.bacterial toxin-mediated foodborne outbreaks, such as those caused by clostridium perfringens, staphylococcus aureus and bacillus cereus, are an important and preventable cause of morbidity and mortality. due to the short incubation period and duration of illness, these outbreaks are often under-reported. this is the first study to describe the epidemiology of bacterial toxin-mediated outbreaks in australia. using data collected between 2001 and 2013, we identify high risk groups and risk factor ...201628043220
chemical composition and biological prospects of essential oils and extracts of aphyllocladus spartioides growing in northwest argentina.aphyllocladus spartioides wedd. is a native and aromatic herb used in traditional medicine, however it is still poorly investigated. in this work, the volatile profile of a. spartioides growing in hornillos-northwest argentina was determined by gc/ms/fid and the phenolic compounds of hydroethanolic and decoction extracts were analyzed by hplc-dad. the antibacterial potential, antioxidant activity and α-glucosidase inhibition activity were checked by in vitro assays. the volatile profile allowed ...201628036133
the liver as another possible target organ for bacillus cereus infection.a case of liver abscess due to bacillus cereus infection in an immunocompetent 59-year-old man is reported. percutaneous drainage and antimicrobial therapy, with vancomycin and levofloxacin afterwards, have been demonstrated to be an appropriate treatment, leading to clinical and radiological cure.201628025629
internal hydration properties of single bacterial endospores probed by electrostatic force microscopy.we show that the internal hydration properties of single bacillus cereus endospores in air under different relative humidity (rh) conditions can be determined through the measurement of its electric permittivity by means of quantitative electrostatic force microscopy (efm). we show that an increase in the rh from 0% to 80% induces a large increase in the equivalent homogeneous relative electric permittivity of the bacterial endospores, from ∼4 up to ∼17, accompanied only by a small increase in t ...201628024372
high occurrence rate and contamination level of bacillus cereus in organic vegetables on sale in retail markets.organic foods have risen in popularity recently. however, the increased risk of bacterial contamination of organic foods has not been fully evaluated. in this study, 100 samples each of organic and conventional fresh vegetables (55 lettuce samples and 45 sprout samples) sold in south korea were analyzed for aerobic bacteria, coliforms, escherichia coli, and bacillus cereus. although the aerobic bacteria and coliform counts were not significantly different between the two farming types (p > 0.05) ...201627992273
nprr, a moonlighting quorum sensor shifting from a phosphatase activity to a transcriptional activator.regulation of biological functions requires factors (proteins, peptides or chemicals) able to sense and translate environmental conditions or any circumstances in order to modulate the transcription of a gene, the stability of a transcript or the activity of a protein. quorum sensing is a regulation mechanism connecting cell density to the physiological state of a single cell. in bacteria, quorum sensing coordinates virulence, cell fate and commitment to sporulation and other adaptation properti ...201628357327
the development of a green approach for the biosynthesis of silver and gold nanoparticles by using panax ginseng root extract, and their biological applications.the biosynthesis of nanoparticles has received attention because of the development of economic and environmentally friendly technology for the synthesis of nanoparticles. the study develops a convenient method for the green synthesis of silver and gold nanoparticles by utilizing fresh root extract of the four-year old panax ginseng plant, and evaluated the antimicrobial applications of silver nanoparticles against pathogenic microorganisms. p. ginseng is a well-known herbal medicinal plant, and ...201625771716
weissella oryzae dc6-facilitated green synthesis of silver nanoparticles and their antimicrobial potential.nanoparticles and nanomaterials are at the prominent edge of the rapidly developing field of nanotechnology. recently, nanoparticle synthesis using biological resources has been found to be a new area with considerable prospects for development. biological systems are the masters of ambient condition chemistry and are able to synthesize nanoparticles by utilizing metal salts. in the perspective of the current initiative to develop green technologies for the synthesis of nanoparticles, microorgan ...201626212222
bacillus cereus biovar anthracis causing anthrax in sub-saharan africa-chromosomal monophyly and broad geographic distribution.through full genome analyses of four atypical bacillus cereus isolates, designated b. cereus biovar anthracis, we describe a distinct clade within the b. cereus group that presents with anthrax-like disease, carrying virulence plasmids similar to those of classic bacillus anthracis. we have isolated members of this clade from different mammals (wild chimpanzees, gorillas, an elephant and goats) in west and central africa (côte d'ivoire, cameroon, central african republic and democratic republic ...201627607836
identification and analysis of informative single nucleotide polymorphisms in 16s rrna gene sequences of the bacillus cereus group.analysis of 16s rrna genes is important for phylogenetic classification of known and novel bacterial genera and species and for detection of uncultivable bacteria. pcr amplification of 16s rrna genes with universal primers produces a mixture of amplicons from all rrna operons in the genome, and the sequence data generally yield a consensus sequence. here we describe valuable data that are missing from consensus sequences, variable effects on sequence data generated from nonidentical 16s rrna amp ...201627582514
the roles of atxa orthologs in virulence of anthrax-like bacillus cereus g9241.atxa is a critical transcriptional regulator of plasmid-encoded virulence genes in bacillus anthracis. bacillus cereus g9241, which caused an anthrax-like infection, has two virulence plasmids, pbcxo1 and pbc210, that each harbor toxin genes and a capsule locus. g9241 also produces two orthologs of atxa: atxa1, encoded on pbcxo1, and atxa2, encoded on pbc210. the amino acid sequence of atxa1 is identical to that of atxa from b. anthracis, while the sequences of atxa1 and atxa2 are 79% identical ...201627490458
bacillus cereus group-type strain-specific diagnostic peptides.the bacillus cereus group consists of eight very closely related species and comprises both harmless and human pathogenic species such as bacillus anthracis, bacillus cereus, and bacillus cytotoxicus. numerous efforts have been undertaken to allow presumptive differentiation of b. cereus group species from one another. however, methods to rapidly and accurately distinguish these species are currently lacking. we confirmed that classical matrix-assisted laser desorption/ionization-time-of-flight ...201627432653
discovery of a unique extracellular polysaccharide in members of the pathogenic bacillus that can co-form with spores.an exopolysaccharide, produced during the late stage of stationary growth phase, was discovered and purified from the culture medium of bacillus cereus, bacillus anthracis, and bacillus thuringiensis when strains were grown in a defined nutrient medium that induces biofilm. two-dimensional nmr structural characterization of the polysaccharide, named pzx, revealed that it is composed of an unusual three amino-sugar sequence repeat of [-3)xylnac4oac(α1-3)glcnaca4oac(α1-3)xylnac(α1-]n the sugar res ...201627402849
anthrax toxin-expressing bacillus cereus isolated from an anthrax-like eschar.bacillus cereus isolates have been described harboring bacillus anthracis toxin genes, most notably b. cereus g9241, and capable of causing severe and fatal pneumonias. this report describes the characterization of a b. cereus isolate, bcfl2013, associated with a naturally occurring cutaneous lesion resembling an anthrax eschar. similar to g9241, bcfl2013 is positive for the b. anthracis pxo1 toxin genes, has a multi-locus sequence type of 78, and a paga sequence type of 9. whole genome sequenci ...201627257909
maldi-tof ms portrait of emetic and non-emetic bacillus cereus group members.the number of foodborne intoxications caused by emetic bacillus cereus isolates has increased significantly. as such, rapid and reliable methods to identify emetic strains appear to be clinically relevant. in this study, intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) was used to differentiate emetic and non-emetic bacilli. the phyloproteomic clustering of 34 b. cereus emetic and 88 non-emetic isolates classified as b. cereus, bacillus thur ...201627196540
plantazolicin is an ultra-narrow spectrum antibiotic that targets the bacillus anthracis membrane.plantazolicin (pzn) is a ribosomally synthesized and post-translationally modified natural product from bacillus methylotrophicus fzb42 and bacillus pumilus. extensive tailoring to twelve of the fourteen amino acid residues in the mature natural product endows pzn with not only a rigid, polyheterocyclic structure, but also antibacterial activity. here we report a remarkably discriminatory activity of pzn toward bacillus anthracis, which rivals a previously-described gamma (γ) phage lysis assay i ...201627152321
cyclic diguanylate regulation of bacillus cereus group biofilm formation.biofilm formation can be considered a bacterial virulence mechanism. in a range of gram-negatives, increased levels of the second messenger cyclic diguanylate (c-di-gmp) promotes biofilm formation and reduces motility. other bacterial processes known to be regulated by c-di-gmp include cell division, differentiation and virulence. among gram-positive bacteria, where the function of c-di-gmp signalling is less well characterized, c-di-gmp was reported to regulate swarming motility in bacillus sub ...201627116468
identification and pathogenic potential of clinical bacillus and paenibacillus isolates.the soil-related bacillus and paenibacillus species have increasingly been implicated in various human diseases. nevertheless, their identification still poses problems in the clinical microbiology laboratory and, with the exception of bacillus anthracis and bacillus cereus, little is known on their pathogenicity for humans. in this study, we evaluated the use of matrix-assisted laser desorption-ionization time of flight mass spectrometry (maldi-tof ms) in the identification of clinical isolates ...201627031639
the phylogeny of bacillus cereus sensu lato.the three main species of the bacillus cereus sensu lato, b. cereus, b. thuringiensis, and b. anthracis, were recognized and established by the early 1900 s because they each exhibited distinct phenotypic traits. b. thuringiensis isolates and their parasporal crystal proteins have long been established as a natural pesticide and insect pathogen. b. anthracis, the etiological agent for anthrax, was used by robert koch in the 19th century as a model to develop the germ theory of disease, and b. ce ...201626999390
extracellular synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 and their biological applications.the present study highlights the microbial synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 strain, in an efficient way. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry, which displayed maximum absorbance at 424nm and 531nm for silver and gold nanoparticles, respectively. the spherical shape of nanoparticles was characterized by field emission transmission electron microscopy. the energy dispersive x-ray spectroscopy and elemental ...201626992796
ebselen and analogs as inhibitors of bacillus anthracis thioredoxin reductase and bactericidal antibacterials targeting bacillus species, staphylococcus aureus and mycobacterium tuberculosis.bacillus anthracis is the causative agent of anthrax, a disease associated with a very high mortality rate in its invasive forms.201626971857
glycosylation of bcla glycoprotein from bacillus cereus and bacillus anthracis exosporium is domain-specific.the spores of the bacillus cereus group (b. cereus, bacillus anthracis, and bacillus thuringiensis) are surrounded by a paracrystalline flexible yet resistant layer called exosporium that plays a major role in spore adhesion and virulence. the major constituent of its hairlike surface, the trimerized glycoprotein bcla, is attached to the basal layer through an n-terminal domain. it is then followed by a repetitive collagen-like neck bearing a globular head (c-terminal domain) that promotes glyco ...201626921321
crystal structure of the bacillus-conserved mazg protein, a nucleotide pyrophosphohydrolase.ba1544 from bacillus anthracis was previously annotated as a transcription factor for the gene cluster ba1554 - ba1558, but has not been experimentally characterized. b. anthracis is an obligate pathogen causing fatal inhalational anthrax, and ba1544 is absolutely conserved in bacillus species, including bacillus cereus, bacillus thuringiensis and bacillus mycoides, with 100% sequence identity. to address the function of ba1544, we performed structural and biochemical studies, which revealed tha ...201626920050
cytotoxic potential of bacillus cereus strains atcc 11778 and 14579 against human lung epithelial cells under microaerobic growth conditions.bacillus cereus, a food poisoning bacterium closely related to bacillus anthracis, secretes a multitude of virulence factors including enterotoxins, hemolysins, and phospholipases. however, the majority of the in vitro experiments evaluating the cytotoxic potential of b. cereus were carried out in the conditions of aeration, and the impact of the oxygen limitation in conditions encountered by the microbe in natural environment such as gastrointestinal tract remains poorly understood. this resear ...201626870026
the regulation of exosporium-related genes in bacillus thuringiensis.bacillus anthracis, bacillus cereus, and bacillus thuringiensis (bt) are spore-forming members of the bacillus cereus group. spores of b. cereus group species are encircled by exosporium, which is composed of an external hair-like nap and a paracrystalline basal layer. despite the extensive studies on the structure of the exosporium-related proteins, little is known about the transcription and regulation of exosporium gene expression in the b. cereus group. herein, we studied the regulation of s ...201626805020
a strategic approach for rapid synthesis of gold and silver nanoparticles by panax ginseng leaves.the study highlights the synthesis of gold nanoparticles and silver nanoparticles by fresh leaves of panax ginseng, an herbal medicinal plant. the reduction of auric chloride and silver nitrate led to the formation of gold and silver nanoparticles within 3 and 45 min, at 80°c, respectively. the developed methodology was rapid, facile, ecofriendly and the utmost significant is quite economical, which did not require subsequent processing for reduction or stabilization of nanoparticles. the nanopa ...201626698271
characterization and antimicrobial application of biosynthesized gold and silver nanoparticles by using microbacterium resistens.various microorganisms were found to be cable of synthesizing gold and silver nanoparticles when gold and silver salts were supplied in the reaction system. the main objective of this study was to evaluate the extracellular synthesis of gold and silver nanoparticles by the type strain microbacterium resistens(t) [kacc14505]. the biosynthesized gold and silver nanoparticles were characterized by ultraviolet-visible spectroscopy (uv-vis), field emission transmission electron micrograph (fe-tem), e ...201626597594
gcogsa-ba: a global core genome snp analysis for bacillus anthracis.as an issue of biosecurity, it is important to identify the origin of a suspected sample to distinguish whether it originated from the release of a bioterrorism agent or from environmental contamination with a virulent agent. here we have developed an analytical pipeline that can infer the phylogenetic position of bacillus cereus group species, including b. anthracis, from next-generation sequencing reads without extensive genomics skills. gcogsa-ba can also detect the existence of anthrax plasm ...201625812430
fermentation of sarshir (kaymak) by lactic acid bacteria: antibacterial activity, antioxidant properties, lipid and protein oxidation and fatty acid profile.the antioxidant and antibacterial activity of fermented sarshir (traditional dairy food), with three probiotic lactobacillus plantarum strains (lp3, af1, and lu5) were investigated. the oxidative stability and lipid profile of non-fermented and fermented sarshir were compared, as well as radical scavenging activity, peroxide, anisidine, and carbonyl values (pv, anv, and cv, respectively).201728349539
[bacillus cereus endocarditis and a probable cutaneous gateway].bacillus cereus is a ubiquitous telluric organism. b. cereus endocarditis is a rare condition seen mostly in prosthetic heart valves and among intravenous drug users. we report a new case of a patient without risk factors and with a good clinical outcome not requiring valve replacement.201728341191
biosorption of heavy metals from aqueous solution by bacteria isolated from contaminated soil.the study was carried out to analyze the heavy metals biosorption potential of bacteria isolated from electroplating industrial effluents contaminated soil. bacterial isolates were screened for their multi-metal biosorption potential against copper, nickel, lead and chromium. bacterial isolate cu4a showed maximum uptake of copper, nickel, lead and chromium in aqueous solution with biosorption efficiency as 87.16 %, 79.62%, 84.92% and 68.12%, respectively. the bacterial strain cu4a was identified ...201728337961
the role of pili in bacillus cereus intraocular infection.bacterial endophthalmitis is a potentially blinding intraocular infection. the bacterium bacillus cereus causes a devastating form of this disease which progresses rapidly, resulting in significant inflammation and loss of vision within a few days. the outer surface of b. cereus incites the intraocular inflammatory response, likely through interactions with innate immune receptors such as tlrs. this study analyzed the role of b. cereus pili, adhesion appendages located on the bacterial surface, ...201728336259
cytotoxicity, bactericidal, and antioxidant activity of sodium alginate hydrosols treated with direct electric current.the aim of the study was to investigate the effect of using direct electric current (dc) of 0, 200, and 400 ma for five minutes on the physiochemical properties, cytotoxicity, antibacterial, and antioxidant activity of sodium alginate hydrosols with different sodium chloride concentrations. the ph, oxidation-reduction potential (orp), electrical conductivity (ec), and available chlorine concentration (acc) were measured. the effect of sodium alginate hydrosols treated with dc on staphylococcus a ...201728327520
cellulase-assisted extraction of polysaccharides from malva sylvestris: process optimization and potential functionalities.enzyme-assisted extraction process of the water-soluble malva sylvestris polysaccharides (msps) was optimized using response surface methodology (rsm). the highest yield (10.40%) of msps was achieved at 5.64% cellulase, 55.65°c temperature, 3.4h time, and 5.22 ph. three homogeneous polysaccharide fractions (msp-1, msp-2, msp-3) were purified by deae-cellulose and sephadex g-100 chromatography, which were composed of galactose, glucuronic acid, arabinose, rhamnose and mannose in different molar r ...201728327424
identification of the lyso-form n-acyl intramolecular transferase in low-gc firmicutes.bacterial lipoproteins are embedded in the cell membrane of both gram-positive and gram-negative bacteria where they serve numerous functions central to cell envelope physiology. lipoproteins are tethered to the membrane by an n-acyl-s-(mono/di)-acyl-glyceryl-cysteine anchor that is variably acylated depending on genera. in several low-gc gram-positive firmicutes, a monoacyl-glyceryl-cysteine with an n-terminal fatty acid (known as the lyso form) has been reported, though how it is formed is unk ...201728320885
biofilm inhibition formation of clinical strains of pseudomonas aeruginosa mutans, photocatalytic activity of azo dye and gc-ms analysis of leaves of lagerstroemia speciosa.the investigation was conducted to analyse the bioactive compounds from the leaf extracts of l. speciosa by gc-ms. the extracts were screened for antibacterial and antibiofilm activities against potential clinical strains. the bioactive compounds from the leaves of l. speciosa were extracted by soxhlet continuous extraction method and their chemical composition was analysed by gas chromatography-mass spectroscopy (gc-ms). the antibacterial activity was evaluated against clinical strain like stap ...201728319869
structure and function of the bacterial communities during rhizoremediation of hexachlorobenzene in constructed wetlands.vertical flow constructed wetlands (vf cws) are considered to be effective for treating organic pollutants. the rhizosphere of macrophytes such as phragmites sp., typha sp. serves as an active and dynamic zone for the microbial degradation of organic pollutants. however, it is still not clear how soil bacterial communities respond to macrophytes and pollutants during the process. for this purpose, the seedlings of phragmites australis and typha angustifolia were planted respectively in the vf cw ...201728316049
development of a liquid chromatography-mass spectrometry based enzyme activity assay for phosphatidylcholine-specific phospholipase c.phosphatidylcholine (pc)-specific phospholipase c (pc-plc) hydrolyzes pc to generate the second messenger 1,2-diacylglycerol (dg) and phosphocholine. pc-plc plays pivotal roles in inflammation, carcinogenesis, tumor progression, atherogenesis, and subarachnoid hemorrhage. although the activity of pc-plc in mammalian tissues was discovered approximately 40 years ago, neither the protein nor its gene has been identified. in the present study, we developed a non-radioactive enzyme activity assay fo ...201728315318
exploiting fruit byproducts for eco-friendly nanosynthesis: citrus × clementina peel extract mediated fabrication of silver nanoparticles with high efficacy against microbial pathogens and rat glial tumor c6 cells.process byproducts from the fruit industry may represent a cheap and reliable source of green reducing agents to be used in current bio-nanosynthesis. this study reports the use of orange (citrus × clementina) peel aqueous extract (ope) for one-pot green synthesis of silver nanoparticles (agnps) with high effectiveness against various microbial pathogens as well as rat glial tumor c6 cells. the effects of various operational parameters on the synthesis of agnps were systematically investigated. ...201728303540
in vitro antibacterial, antifungal and antioxidant activities of eucalyptus spp. leaf extracts related to phenolic composition.the crude methanolic extracts from leaves of eucalyptus camaldulensis l., e. camaldulensis var obtusa and e. gomphocephala grown in egypt were investigated to explore their chemical composition as well as their antibacterial, antifungal and antioxidant activities. major phenolics found were ellagic acid, quercetin 3-o-rhamnoside, quercetin 3-o-b-d-glucuronide, caffeic acid and chlorogenic acid. the antioxidant activities were examined by the 2,2'-diphenypicrylhydrazyl (dpph) and β-carotene-linol ...201728299978
chemical composition and biological properties of satureja avromanica maroofi.satureja avromanica is an indigenous plant which is frequently used as a spice in avraman-kurdistan region of iran. the present study aimed to investigate the chemical composition, antimicrobial and antioxidant properties of the s. avromanica. in addition, rosmarinic acid and total phenolic content of s. avromanica was assessed by spectrophotometric method and hptlc. the essential oil and methanolic extract were isolated by hydrodistillation and maceration methods, respectively. a total of 32 co ...201728298686
induced systemic resistance against botrytis cinerea by bacillus cereus ar156 through a ja/et- and npr1-dependent signaling pathway and activates pamp-triggered immunity in arabidopsis.induced resistance response is a potent and cost effective plant defense against pathogen attack. the effectiveness and underlying mechanisms of the suppressive ability by bacillus cereus ar156 to pseudomonas syringae pv. tomato dc3000 (pst dc3000) in arabidopsis has been investigated previously; however, the strength of induced systemic resistance (isr) activity against botrytis cinerea remains unknown. here, we show that root-drench application of ar156 significantly reduces disease incidence ...201728293243
prevalence, virulence factor genes and antibiotic resistance of bacillus cereus sensu lato isolated from dairy farms and traditional dairy products.b. cereus are of particular interest in food safety and public health because of their capacity to cause food spoilage and disease through the production of various toxins. the aim of this study was to determine the prevalence, virulence factor genes and antibiotic resistance profile of b. cereus sensu lato isolated from cattle grazing soils and dairy products in ghana. a total of 114 samples made up of 25 soil collected from cattle grazing farm land, 30 raw milk, 28 nunu (yoghurt-like product) ...201728288581
genetic and genomic diversity of nheabc locus from bacillus strains.non-hemolytic enterotoxin (nhe), a tri-partite, proteinaceous toxin encoded by contiguous nhea, nheb and nhec genes of bacillus cereus sensu lato (b. cereus s.l.), is considered to be associated with the foodborne diarrheic syndrome. however, b. cereus s.l. includes a number of closely related strains, and the occurrence of nhe among them, and other members of bacillus is unclear. consequently, we aimed to determine the distribution and evolution of nhe within bacillus by confirming the presence ...201728283680
complete genome sequences of bacillus phages dirtybetty and kida.we report here the genome sequences of two bacteriophages of the bacillus cereus group, dirtybetty and kida. these bacteriophages are double-stranded dna-containing myoviridae isolated from soil samples using bacillus thuringiensis subsp. kurstaki as their host bacteria.201728280018
antibacterial activity and synergistic antibacterial potential of biosynthesized silver nanoparticles against foodborne pathogenic bacteria along with its anticandidal and antioxidant effects.silver nanoparticles plays a vital role in the development of new antimicrobial substances against a number of pathogenic microorganisms. these nanoparticles due to their smaller size could be very effective as they can improve the antibacterial activity through lysis of bacterial cell wall. green synthesis of metal nanoparticles using various plants and plant products has recently been successfully accomplished. however, few studies have investigated the use of industrial waste materials in nan ...201728261161
trimethylamine removal by plant capsule of sansevieria kirkii in combination with bacillus cereus en1.trimethylamine (tma) contamination produces a strong "fishy" odor and can cause pathological changes in humans. by screening native microorganisms from sansevieria kirkii exposed to 100 ppm tma, it was shown that endophytic bacteria number 1 (en1) and number 2 (en2) have a higher tma tolerance and removal capacity than other bacteria species in a closed system. in addition, en1 and en2 demonstrated the ability to produce high quantities of indole-3-acetic acid (iaa) and use 1-aminocyclopropane-1 ...201728258430
the bacterial sequential markov coalescent.bacteria can exchange and acquire new genetic material from other organisms directly and via the environment. this process, known as bacterial recombination, has a strong impact on the evolution of bacteria, for example leading to the spread of antibiotic resistance across clades and species, and to the avoidance of clonal interference. recombination hinders phylogenetic and transmission inference because it creates patterns of substitutions (homoplasies) inconsistent with the hypothesis of a si ...201728258183
microbiological quality of raw milk attributable to prolonged refrigeration conditions.refrigerated storage of raw milk is a prerequisite in dairy industry. however, temperature abused conditions in the farming and processing environments can significantly affect the microbiological quality of raw milk. thus, the present study investigated the effect of different refrigeration conditions such as 2, 4, 6, 8, 10 and 12 °c on microbiological quality of raw milk from three different dairy farms with significantly different initial microbial counts. the bacterial counts (bc), protease ...201728252354
impact of endophytic colonization patterns on zamioculcas zamiifolia stress response and in regulating ros, tryptophan and iaa levels under airborne formaldehyde and formaldehyde-contaminated soil conditions.deeper understanding of plant-endophyte interactions under abiotic stress would provide new insights into phytoprotection and phytoremediation enhancement. many studies have investigated the positive role of plant-endophyte interactions in providing protection to the plant against pollutant stress through auxin (indole-3-acetic acid (iaa)) production. however, little is known about the impact of endophytic colonization patterns on plant stress response in relation to reactive oxygen species (ros ...201728246037
should we care about transmission of infectious bacterial agents through serum handling?immunological assays are valuable diagnostic tools to detect infections due to most of bacterial microorganisms. however, the question is how much safe are the common serum samples used as sources of antibodies in these assays? here, we experimentally followed the issue. 10-fold serial dilutions of two gram-positive, staphylococcus aureus and bacillus cereus, and one gram-negative bacteria, escherichia coli, were prepared and spiked into freshly taken blood samples of human and four animal speci ...201728242424
the antibacterial activity of extracts of nine plant species with good activity against escherichia coli against five other bacteria and cytotoxicity of extracts.the development of antibiotic resistant bacteria stems from a number of factors, including inappropriate use of antibiotics in human and animal health and their prolonged use as growth promoters at sub-clinical doses in poultry and livestock production. we were interested in investigating plants that could be useful in protecting humans or animals against diarrhoea. we decided to work on extracts of nine plant species with good activity against escherichia coli based on earlier work in the phyto ...201728241818
draft genome sequences of enterotoxigenic bacillus cereus strains obtained from powdered infant formula.we introduce the draft genome sequences of five enterotoxigenic bacillus cereus strains: bc 12, bc 67, bc 111, bc 112, and bc 113, which were obtained from powdered infant formula. the genome sizes of the strains ranged from 5.5 to 5.8 mb, and the g+c contents were ~35.2%.201728232440
synthetic investigation, physicochemical characterization and antibacterial evaluation of ternary bi(iii) systems with hydroxycarboxylic acid and aromatic chelator substrates.due to its physical and chemical properties, bismuth (bi(iii)) is widely used in the treatment of several gastrointestinal and skin diseases, and infections caused by bacteria. herein, its known antimicrobial potential was taken into consideration in the synthesis of two new hybrid ternary materials of bi(iii) with the physiological α-hydroxycarboxylic glycolic acid and 1,10-phenanthroline (phen), [bi2(c2h2o3)2(c2h3o3)(no3)]n(.) nh2o (1) and [bi(c12h8n2)(no3)4](c10h8n4) (2), aiming at improving ...201728231455
spectroscopy analysis and molecular dynamics studies on the binding of penicillin v and sulbactam to beta-lactamase ii from bacillus cereus.the molecular recognition and interaction of beta-lactamase ii from bacillus cereus (bc ii) with penicillin v (pv) and sulbactam (sul) especially conformational changes of bc ii in the binding process were studied through spectroscopy analysis in combination with molecular dynamics (md) simulation. the results show that in the binding process, a new coordination bond is observed between the zn2 of bc ii and the carboxyl-o of pv or sul by replacing his204. electrostatic interaction between zn2 an ...201728219797
microbiological analysis, antimicrobial activity, and heavy-metals content of jordanian ma'in hot-springs water.ma'in hot springs are known as sites of balneotherapy. however, little is known about their microbiology and chemistry. in this study, we aim at evaluating the antimicrobial activity of ma'in hot-springs water (mhsw), studying its microbiology, and determining its physicochemical properties including the heavy metals content. therefore, water samples were collected from ma'in hot springs and tested for antimicrobial activity using agar diffusion method. water was then cultivated on nutrient agar ...201728214187
heat-resistance of psychrotolerant bacillus cereus vegetative cells.spores of psychrotolerant strains of the foodborne pathogen bacillus cereus can multiply during storage of cooked or pasteurized, refrigerated foods and can represent a risk if these cells are not eliminated during reheating of food product before consumption. we determined the heat-resistance of psychrotolerant b. cereus vegetative cells at different heating temperatures in laboratory medium and compared it with that of thermotolerant b. cereus vegetative cells. the z values, based on times for ...201728213026
kinneretia thg-sqi4 mediated biosynthesis of silver nanoparticles and its antimicrobial efficacy.simple, facile, effective approach for the biosynthesis of silver nanoparticles using kinneretia species and its antimicrobial activity against human pathogens has been demonstrated in this study. kinneretia thg-sqi4 has been isolated from soil sample collected from shangqui, china. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry (uv-vis), field emission transmission electron microscopy (fe-tem), energy dispersive x-ray spectroscopy (edx), elemental mapp ...201728211298
improvement of polymyxin-egg yolk-mannitol-bromothymol blue agar for the enumeration and isolation of bacillus cereus in various foods.a modified polymyxin-egg yolk-mannitol-bromothymol blue agar (mpemba) was developed by supplementing polymyxin-egg yolk-mannitol-bromothymol blue agar (pemba) with trimethoprim to improve the selectivity for and recoverability of bacillus cereus from naturally and artificially contaminated food samples. the number of b. cereus in mpemba was significantly higher than in pemba, indicating better recoverability (p < 0.05) in red pepper powder (pemba 0.80 ± 0.22 log cfu/g versus mpemba 1.95 ± 0.17 l ...201728207304
isolation and characterization of bacillus cereus bacteriophages from foods and soil.the aim of this study was to isolate and characterize bacillus cereus bacteriophages of various origins. twenty-seven bacteriophages against b. cereus were isolated from various korean traditional fermented foods and soils. plaque size, transmission electron microscopy, virulence profile, and in vitro lytic activity of bacteriophage isolates were examined. transmission electron microscopy confirmed b. cereus bacteriophages belonging to the family siphoviridae. among b. cereus bacteriophages with ...201728205130
compensatory evolution of intrinsic transcription terminators in bacillus cereus. 201728201729
antibiogram of bacteria isolated from automated teller machines in hamadan, west iran.aim: bacteria are ubiquitous in the environment. in keeping with the continued expansion of urbanization and the growing population, an increasing number of people use automated banking, i.e. automated teller machines (atms). the aim of this study was to investigate the bacterial contamination and its antibiotic sensitivity on computer keyboards located at atms in hamadan province, iran. method: out of 360 atms at four locations in hamadan, 96 were randomly selected for this study. the antibioti ...201728197394
spoilage of microfiltered and pasteurized extended shelf life milk is mainly induced by psychrotolerant spore-forming bacteria that often originate from recontamination.premature spoilage and varying product quality due to microbial contamination still constitute major problems in the production of microfiltered and pasteurized extended shelf life (esl) milk. spoilage-associated bacteria may enter the product either as part of the raw milk microbiota or as recontaminants in the dairy plant. to identify spoilage-inducing bacteria and their routes of entry, we analyzed end products for their predominant microbiota as well as the prevalence and biodiversity of psy ...201728197147
design, synthesis, and antibacterial activities of novel heterocyclic arylsulphonamide derivatives.design, synthesis, and antibacterial activities of a series of arylsulphonamide derivatives as probable peptide deformylase (pdf) inhibitors have been discussed. compounds have been designed following lipinski's rule and after docking into the active site of pdf protein (pdb code: 1g2a) synthesized later on. furthermore, to assess their antibacterial activity, screening of the compound was done in vitro conditions against gram-positive and gram-negative bacterial strains. in silico, studies reve ...201728194576
substance p and calcitonin gene-related peptide: key regulators of cutaneous microbiota homeostasis.neurohormones diffuse in sweat and epidermis leading skin bacterial microflora to be largely exposed to these host factors. bacteria can sense a multitude of neurohormones, but their role in skin homeostasis was only investigated recently. the first study focused on substance p (sp), a neuropeptide produced in abundance by skin nerve terminals. sp is without effect on the growth of gram-positive (bacillus cereus, staphylococcus aureus, and staphylococcus epidermidis) and gram-negative (pseudomon ...201728194136
emergence of multi drug resistance among soil bacteria exposing to insecticides.impacts of pesticide exposure on the soil microbial flora and cross resistance to antibiotics have not been well documented. development of antibiotic resistance is a common issue among soil bacteria which are exposing to pesticides continuously at sub-lethal concentration. the present study was focused to evaluate the correlation between pesticide exposures and evolution of multi drug resistance among isolates collected from soil applied with insecticides. twenty five insecticide (monochrotopho ...201728192223
an assessment of the microbiological quality of liver-based pâté in england 2012-13: comparison of samples collected at retail and from catering businesses.the purpose of this study was to investigate the microbiological quality of liver pâté. during 2012-13, a total of 870 samples, unrelated to the investigation of food-poisoning outbreaks, were collected either at retail (46%), catering (53%) or the point of manufacture (1%) and were tested using standard methods to detect salmonella spp. or campylobacter spp., and to enumerate for listeria spp., including listeria monocytogenes, clostridium perfringens, coagulase-positive staphylococci including ...201728190406
functional expression and purification of recombinant hepcidin25 production in escherichia coli using sumo fusion technology.hepcidin25 is a small cysteine-rich peptide hormone known as a new class of antimicrobial peptides. the purpose of the present study was to express, purify and investigate the antibacterial properties of recombinant human hepcidin25 protein production in escherichia coli. human hepcidin25 gene was optimized and fused to a small ubiquitin-related modifier (sumo) gene for higher expression. then sumo-hepcidin25 was cloned into the pet-32a (+) vector and expressed in e. coli origami. the fusion pro ...201728188870
molecular recognition and binding of beta-lactamase ii from bacillus cereus with penicillin v and sulbactam by spectroscopic analysis in combination with docking simulation.the molecular recognition and binding interaction of beta-lactamase ii from bacillus cereus (bc ii) with penicillin v (pv) and sulbactam (sul) at 277 k were studied by spectroscopic analysis and molecular docking. the results showed that a non-fluorescence static complex was separately formed between bc ii and two ligands, the molecular ratio of bc ii to pv or sul was both 1:1 in the binding and the binding constants were 2.00 × 10(6) and 3.98 × 10(5) (l/mol), respectively. the negative free ene ...201728185399
enhanced extracellular production of recombinant proteins in escherichia coli by co-expression with bacillus cereus phospholipase c.our laboratory has reported a strategy for improving the extracellular production of recombinant proteins through co-expression with thermobifida fusca cutinase, which increases membrane permeability via its phospholipid hydrolysis activity. however, the foam generated by the lysophospholipid product makes the fermentation process difficult to control in a fermentor. phospholipase c (plc) catalyzes the hydrolysis of phospholipids to produce sn1,2-diacylglycerides and organic phosphate, which do ...201728178978
alliacane sesquiterpenoids from submerged cultures of the basidiomycete inonotus sp. bcc 22670.nine alliacane sesquiterpenoids, inonoalliacanes a-i, were isolated from culture broth of the basidiomycete inonotus sp. bcc 22670. the structures were elucidated on the basis of nmr spectroscopic and mass spectrometry data. the absolute configuration of inonoalliacane f was determined by application of the modified mosher's method. inonoalliacane a, the most abundant sesquiterpene constituent, exhibited moderate antibacterial activity against bacillus cereus, whereas inonoalliacane b showed ant ...201728173949
microbial interactions in the phyllosphere increase plant performance under herbivore biotic stress.the phyllosphere supports a tremendous diversity of microbes and other organisms. however, little is known about the colonization and survival of pathogenic and beneficial bacteria alone or together in the phyllosphere across the whole plant life-cycle under herbivory, which hinders our ability to understand the role of phyllosphere bacteria on plant performance. we addressed these questions in experiments using four genetically and biogeographically diverse accessions of arabidopsis thaliana, t ...201728163703
epidihydropinidine, the main piperidine alkaloid compound of norway spruce (picea abies) shows promising antibacterial and anti-candida activity.this study reports for the first time promising antibacterial and antifungal effects of epidihydropinidine, the major piperidine alkaloid in the needles and bark of norway spruce, picea abies (l.) karsten. epidihydropinidine was growth inhibitory against all bacterial and fungal strains used in our investigation, showing the lowest mic value of 5.37μg/ml against pseudomonas aeruginosa, enterococcus faecalis, candida glabrata and c. albicans. epidihydropinidine was nearly three times more active ...201728163074
draft genome sequences of 18 psychrotolerant and 2 thermotolerant strains representative of particular ecotypes in the bacillus cereus group.bacteria from the bacillus cereus group exhibit genetic and physiological diversity through different ecotypes. here, we present the draft genome sequences of 20 bacterial strains belonging to the contrasted psychrotolerant and thermotolerant ecotypes.201728153905
the essential oil from the fruits of the brazilian spice xylopia sericea a. st.-hil. presents expressive in-vitro antibacterial and antioxidant activity.the aims of this study were to investigate the chemical composition and the antioxidant activity and antibacterial activity of the essential oil of xylopia sericea fruits (oxs). the fruits of this species are popularly used for medicinal purposes, and as a condiment in food preparation.201728134988
whole-genome resequencing of bacillus cereus and expression of genes functioning in sodium chloride stress.bacillus cereus is one of the most common opportunistic pathogens responsible for various foodborn diseases. to investigate the regulatory mechanism of b. cereus under high osmotic pressure, two b. cereus strains b25 and b26 were isolated from the industrial soy sauce residue containing high-salt concentration. resequencing was performed by illumina/solexa platform and 13,646 snps and 434 indels were identified as common variants between b25 and b26 against reference genome, followed by cog, go, ...201728131953
draft genome sequence of flavobacterium johnsoniae ci04, an isolate from the soybean rhizosphere.flavobacterium johnsoniae ci04 was coisolated with bacillus cereus from a root of a field-grown soybean plant in arlington, wi, and selected as a model for studying commensalism between members of the cytophaga-flavobacterium-bacteroides group and b. cereus here we report the draft genome sequence of f. johnsoniae ci04 obtained by illumina sequencing.201728126949
hptlc fingerprint profile, in vitro antioxidant and evaluation of antimicrobial compound produced from brevibacillus brevis-egs9 against multidrug resistant staphylococcus aureus.in the present study, in vitro antimicrobial activity of brevibacillus brevis egs9 against multi drug resistant staphylococcus aureus (mdrsa) and to investigate the antimicrobial, antioxidant activity and hptlc finger print profile of brevibacillus brevis egs9. primary screening was done using by cross streak method against multi drug resistant staphylococcus aureus. the bioactive metabolites were extracted from brevibacillus brevis egs9 using ethyl acetate extraction. ethyl acetate extract show ...201727939872
sodium alginate and gum acacia hydrogels of zno nanoparticles show wound healing effect on fibroblast cells.an ideal biomaterial for wound dressing applications should possess antibacterial and anti-inflammatory properties without any toxicity to the host cells while providing the maximum healing activity. zinc oxide nanoparticles (znonps) possess antimicrobial activity and enhance wound healing, but the questions regarding their safety arise before application to the biological systems. we synthesized znonps-loaded-sodium alginate-gum acacia hydrogels (saga-znonps) by cross linking hydroxyl groups of ...201727939272
alleviation of heavy metals toxicity by the application of plant growth promoting rhizobacteria and effects on wheat grown in saline sodic field.the aim of the study was to determine tolerance of plant growth promoting rhizobacteria (pgpr) in different concentrations of cu, cr, co, cd, ni, mn, and pb and to evaluate the pgpr-modulated bioavailability of different heavy metals in the rhizosphere soil and wheat tissues, grown in saline sodic soil. bacillus cereus and pseudomonas moraviensis were isolated from cenchrus ciliaris l. growing in the khewra salt range. seven-day-old cultures of pgpr were applied on wheat as single inoculum, co-i ...201727936865
bacteria and hoverflies (diptera: syrphidae) in tree hollows from the iberian mediterranean forest.saproxylic insect communities inhabiting tree hollows in mediterranean forests depend on a combination of physical characteristics and interactions occurring between community member species. despite the need to preserve these organisms, little is known about their interrelationships, in particular those relationships between saproxylic insects and microbiota occurring in these microhabitats. in tree hollows of quercus rotundifolia lamark that hold water and contain dead leaves, abundant microbi ...201728025224
cmc stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics.in the present study silver nanoparticles were synthesized by reduction of agno3 using aqueous cmc solution, which acts as both reducing and capping agent. the formation of agno3 nanoparticles was observed visually by color change and these nanoparticles were characterized through uv-vis spectroscopy, ftir, xrd, sem, eds and afm. the ftir peaks observed to be ranging from 3300 to 605cm(-1). the afm image clearly showed the surface morphology of well dispersed nanoparticles. sem image illustrates ...201728024537
inactivation and changes in metabolic profile of selected foodborne bacteria by 460 nm led illumination.the objective of this study was to investigate the effect of 460 nm light-emitting diode (led) on the inactivation of foodborne bacteria. additionally, the change in the endogenous metabolic profile of led illuminated cells was analyzed to understand the bacterial response to the led illumination. six different species of bacteria (bacillus cereus, listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, pseudomonas aeruginosa and salmonella typhimurium) were illuminated with 460 ...201728040158
antibiotic discovery throughout the small world initiative: a molecular strategy to identify biosynthetic gene clusters involved in antagonistic activity.the emergence of bacterial pathogens resistant to all known antibiotics is a global health crisis. adding to this problem is that major pharmaceutical companies have shifted away from antibiotic discovery due to low profitability. as a result, the pipeline of new antibiotics is essentially dry and many bacteria now resist the effects of most commonly used drugs. to address this global health concern, citizen science through the small world initiative (swi) was formed in 2012. as part of swi, stu ...201728110506
bacillus cereus bacteraemia: comparison between haematologic and nonhaematologic patients.bacillus cereus bacteraemia can be severe, especially among patients with haematologic malignancy. we retrospectively reviewed first episodes of true b. cereus bacteraemia (more than one positive bottle plus signs of infection) at our institution between 1997 and 2013 with the aim to compare haematologic versus nonhaematologic patients and analyse episodes with complicated outcome. among 56 episodes of positive-blood cultures for b. cereus, 21 were considered significant. median age was 54 years ...201728050250
bioremediation of petroleum wastewater by hyper-phenol tolerant bacillus cereus: preliminary studies with laboratory-scale batch process.petroleum wastewater samples from oil refinery and oil exploration site were treated by hyper phenol-tolerant bacillus cereus (akg1 and akg2) in laboratory-scale batch process to assess their bioremediation efficacy. quality of the treated wastewater samples were analyzed in terms of removal of chemical oxygen demand (cod), total organic carbon (toc) and ammonium nitrogen content, and improvement of biological oxygen demand (bod). adaptation of these bacteria to the toxic environment through str ...201728095107
artp mutation and genome shuffling of abe fermentation symbiotic system for improvement of butanol production.butanol is an ideal renewable biofuel which possesses superior fuel properties. previously, butanol-producing symbiotic system tsh06 was isolated in our lab, with microoxygen tolerance ability. to boost butanol yield for large-scale industrial production, tsh06 was used as parental strain and subjected to atmospheric and room temperature plasma (artp) and four rounds of genome shuffling (gs). artp mutant and gs strain were co-cultured with facultative anaerobic bacillus cereus tsh2 to form a sym ...201728074224
biochemical and molecular study of carpobrotus edulis bioactive properties and their effects on dugesia sicula (turbellaria, tricladida) regeneration.the traditional medicinal properties of carpobrotus edulis are well recognized, particularly in tunisia where it is used for wound healing. thus, in this study, biochemical and molecular properties of its leaves' bioactive aqueous-acetone extract were investigated. the total phenolic content (tpc) of the extract was estimated to be 184 ± 5 mg/100 g of fresh matter (fm). the qualitative and quantitative polyphenolic profile was determined by ultra performance liquid chromatography with diode arra ...201728064427
terphenyl derivatives and drimane - phathalide/isoindolinones from hypoxylon fendleri bcc32408.the genus hypoxylon, a member of the family xylariaceae, has been known to produce significant secondary metabolites in terms of chemical diversity. moreover, the compounds isolated can also be used as chemotaxonomic characters for differentiation among the two sections, which are sect. annulata and sect. hypoxylon. in our continuing chemical screening programme for novel compounds, the crude extracts of h. fendleri bcc32408 gave significant chemical profiles in hplc analyses. thus, the chemical ...201728384525
characterization of plasma membrane ceramides by super-resolution microscopy.the sphingolipid ceramide regulates cellular processes such as differentiation, proliferation, growth arrest, and apoptosis. ceramide-rich membrane areas promote structural changes within the plasma membrane that segregate membrane receptors and affect membrane curvature and vesicle formation, fusion, and trafficking. ceramides were labeled by immunocytochemistry to visualize their distribution on the plasma membrane of different cells with virtually molecular resolution by direct stochastic opt ...201728379629
a new anthraquinone and a new naphthoquinone from the whole plant of spermacoce latifolia.a phytochemical study on the whole plant of spermacoce latifolia led to the isolation of a new anthraquinone, 1,2,6-trihydroxy-5-methoxy-9,10-anthraquinone (1), and a new naphthoquinone, (2r)-6-hydroxy-7-methoxy-dehydroiso-α-lapachone (2), together with three known anthraquinones (3-5). their structures were established on the basis of detailed spectroscopic analysis, including one- and two-dimensional nmr, esi-ms, and hr-esi-ms techniques. all the compounds were isolated from s. latifolia for t ...201728357881
n-heterocyclic choline analogues based on 1,2,3,4-tetrahydro(iso)quinoline scaffold with anticancer and anti-infective dual action.pharmacological effects of biologically active "small molecules" can be improved by their targeted modification, which affects drug delivery and interaction with tumor cells and microorganisms. we aimed to evaluate anticancer and antimicrobial activity of lipid-like choline derivatives modified via simultaneous introduction of tetrahydro(iso)quinoline based pharmacophore system at nitrogen atom and long chain alkyl substituent at oxygen atom.201728364698
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