Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [maintenance of genome stability though ubc13-dependent ubiquitination]. | 2009 | 19697864 | |
| gpr50 interacts with neuronal nogo-a and affects neurite outgrowth. | g protein-coupled receptors (gpcrs) form a link between the cell and their environment when signalling pathways are activated upon ligand binding. however, the ligands and functions for many gpcrs remain to be determined. we sought to understand the function of one such orphan, g protein-coupled receptor 50 (gpr50), through identification of protein interactors. gpr50 was previously discovered as a candidate gene for psychiatric illness and lipid metabolism. here, we identified neurite outgrowth ... | 2009 | 19699797 |
| synthesis, raman, ft-ir, nmr spectroscopic characterization, antimicrobial activity, cytotoxicity and dna binding of new mixed aza-oxo-thia macrocyclic compounds. | series of new mixed aza-oxo-thia macrocyclic ligands 1,9(2,6)-ditriazina-2,8,10,16-tetraaza-3,7,11,15-tetraoxo-5,13-dithia-cyclohexadecaphan-1(4),9(4)-diphenyl (l(1)); 1,10(2,6)-ditri azina-2,9,11,18-tetraaza-3,8,12,17-tetraoxo-5,6,14,15-tetrathia-cyclooctadecaphan-1(4),10(4)-diphenyl (l(2)); 1,11(2,6)-ditriazina-2,10,12,20-tetraaza-3,9,13,19-tetraoxo-6,16-dithia-cyclocosa-phan-1(4),11(4)-diphenyl (l(3)); 1,12(2,6)-ditriazina-2,11,13,22-tetraaza-3,10,14,21-tetraoxo-6,7,17,18-tetrathia-cyclodocos ... | 2009 | 19700225 |
| human pif1 helicase unwinds synthetic dna structures resembling stalled dna replication forks. | pif-1 proteins are 5'-->3' superfamily 1 (sf1) helicases that in yeast have roles in the maintenance of mitochondrial and nuclear genome stability. the functions and activities of the human enzyme (hpif1) are unclear, but here we describe its dna binding and dna remodeling activities. we demonstrate that hpif1 specifically recognizes and unwinds dna structures resembling putative stalled replication forks. notably, the enzyme requires both arms of the replication fork-like structure to initiate ... | 2009 | 19700773 |
| the localization of the golgin gcc185 is independent of rab6a/a' and arl1. | mammalian golgins of the trans-golgi network (tgn) are small g protein effectors that are required for membrane transport and contain a golgi targeting c-terminal grip domain. the localization of two tgn golgins, p230/golgin-245 and golgin-97, is mediated by the small gtpase arl1, whereas recruitment of the tgn golgin gcc185 is controversial. recently, gcc185 was proposed to localize to the golgi by the co-operation of two small gtpases, rab6a/a' and arl1 (burguete et al., 2008), a model based p ... | 2009 | 19703403 |
| nascent: an automatic protein interaction network generation tool for non-model organisms. | large quantity of reliable protein interaction data are available for model organisms in public depositories (e.g., mint, dip, hprd, interact). most data correspond to experiments with the proteins of saccharomyces cerevisiae, drosophila melanogaster, homo sapiens, caenorhabditis elegans, escherichia coli and mus musculus. for other important organisms the data availability is poor or non-existent. here we present nascent, a completely automatic web-based tool and also a downloadable java progra ... | 2009 | 19707301 |
| the ccr4-not complex physically and functionally interacts with tramp and the nuclear exosome. | ccr4-not is a highly conserved multi-protein complex consisting in yeast of 9 subunits, including not5 and the major yeast deadenylase ccr4. it has been connected functionally in the nucleus to transcription by rna polymerase ii and in the cytoplasm to mrna degradation. however, there has been no evidence so far that this complex is important for rna degradation in the nucleus. | 2009 | 19707589 |
| the redox-sensing quinone reductase lot6p acts as an inducer of yeast apoptosis. | mammalian nad(p)h:quinone oxidoreductases such as human nqo1 act as inducers of apoptosis. quinone reductases generated interest over the last decade due to their proposed function in the oxidative stress response. furthermore, human nqo1 was reported to regulate p53 stability and p53-dependent apoptosis through regulation of cellular oxidation-reduction events. in this study, we have used low concentrations of hydrogen peroxide (0.4 and 0.6 mm) to induce apoptosis-like cell death in wild type, ... | 2009 | 19709309 |
| yeast genome analysis identifies chromosomal translocation, gene conversion events and several sites of ty element insertion. | paired end mapping of chromosomal fragments has been used in human cells to identify numerous structural variations in chromosomes of individuals and of cancer cell lines; however, the molecular, biological and bioinformatics methods for this technology are still in development. here, we present a parallel bioinformatics approach to analyze chromosomal paired-end tag (chrompet) sequence data and demonstrate its application in identifying gene rearrangements in the model organism saccharomyces ce ... | 2009 | 19710036 |
| discovery of chemical modulators of a conserved translational control pathway by parallel screening in yeast. | eukaryotic initiation factor 2 (eif2) b is a guanine nucleotide exchange factor that plays a central role in translation initiation and its control, especially in response to diverse cellular stresses. in addition, inherited mutations in human eif2b subunits cause a fatal brain disorder commonly called childhood ataxia with central nervous system hypomyelination or leukoencephalopathy with vanishing white matter. in yeast, inhibiting activity of eif2b up-regulates expression of the transcription ... | 2009 | 19715453 |
| streptococcus mutans competence-stimulating peptide inhibits candida albicans hypha formation. | the oral cavity is colonized by microorganisms growing in biofilms in which interspecies interactions take place. streptococcus mutans grows in biofilms on enamel surfaces and is considered one of the main etiological agents of human dental caries. candida albicans is also commonly found in the human oral cavity, where it interacts with s. mutans. c. albicans is a polymorphic fungus, and the yeast-to-hypha transition is involved in virulence and biofilm formation. the aim of this study was to in ... | 2009 | 19717744 |
| rna-based computation in live cells. | man-made molecular 'computers' that operate inside live cells will enable unprecedented level of control over cellular physiology. a promising approach to building these computers uses rna molecules and rna-based regulation. rna naturally lends itself to create 'digital' molecular networks that embody standardized (normal) forms of logic functions. the network's inputs, that may or may not be inverted by single-input not logic gates, feed into multi-input and gates whose outputs are in turn inte ... | 2009 | 19720518 |
| ambisome and amphotericin b inhibit the initial adherence of candida albicans to human epithelial cell lines, but do not cause yeast detachment. | candida biofilms with reduced susceptibility to conventional antifungals are sensitive to lipid formulations of amphotericin b (amb). we examined the effect of the liposomal amb formulation, ambisome, and free amb on the adherence of c. albicans to hela cervical carcinoma and hsc-3 oral squamous cell carcinoma cells. | 2009 | 19721394 |
| biochemical characterisation of the swi/snf family member hltf. | hltf is highly similar in domain organisation to yeast rad5. we identify ptip and rpa70, both involved in dna replication and dna repair, as hltf-interacting proteins although cells depleted of hltf did not show defects in cellular responses to dna damage. in vitro, hltf has atpase activity and e3 ubiquitin ligase activity with a range of e2 ubiquitin conjugating enzymes. hltf expression is severely reduced in a range of cancer cells, and we suggest that the hltf antibodies generated in this stu ... | 2009 | 19723507 |
| establish a recombinant yeast detection system to study the effect of mip on transactivation function of hmaff in us2-driven gene transcription. | the human gene maff (hmaff) is a member of bzip transcription factor maf family, but it alone cannot activate its target genes. in 2006, a novel hmaff interacting protein (mip) was identified. transient transfection assay in hela cells suggested that co-expression of mip and hmaff could activate us2-driven transcription. in this work, we constructed a series of plasmids and transformed ym4271 yeast strain to establish a recombinant yeast detection system. in this system, mip's expression level c ... | 2009 | 19723544 |
| crystallization and preliminary x-ray diffraction studies of the ubiquitin-like (ubl) domain of the human homologue a of rad23 (hhr23a) protein. | human homologue a of rad23 (hhr23a) plays dual roles in dna repair as well as serving as a shuttle vehicle targeting polyubiquitinated proteins for degradation. its n-terminal ubiquitin-like (ubl) domain interacts with the 19s proteasomal cap and provides the docking mechanism for protein delivery. pyramidal crystals of the ubl domain of hhr23a were obtained by the hanging-drop vapour-diffusion method with ammonium sulfate as the crystallizing agent. the crystals diffracted to beyond 2 a resolut ... | 2009 | 19724136 |
| set domain group25 encodes a histone methyltransferase and is involved in flowering locus c activation and repression of flowering. | covalent modifications of histone lysine residues by methylation play key roles in the regulation of chromatin structure and function. in contrast to h3k9 and h3k27 methylations that mark repressive states of transcription and are absent in some lower eukaryotes, h3k4 and h3k36 methylations are considered as active marks of transcription and are highly conserved in all eukaryotes from yeast (saccharomyces cerevisiae) to homo sapiens. paradoxically, protein complexes catalyzing h3k4 and h3k36 met ... | 2009 | 19726574 |
| interaction of postsynaptic density protein-95 with nmda receptors influences excitotoxicity in the yeast artificial chromosome mouse model of huntington's disease. | evidence suggests that nmda-type glutamate receptors contribute to degeneration of striatal medium-sized spiny neurons (msns) in huntington's disease (hd). previously, we demonstrated that nmda receptor (nmdar)-mediated current and/or toxicity is increased in msns from the yeast artificial chromosome (yac) transgenic mouse model expressing polyglutamine (polyq)-expanded (mutant) full-length human huntingtin (htt). others have shown that membrane-associated guanylate kinases (maguks), such as psd ... | 2009 | 19726651 |
| identification of novel partner proteins of pcbp1. | pcbp1 is a family member of heterogeneous nuclear ribonucleoproteins (hnrnps) that belong to rna-binding proteins and bear three kh domains. the protein plays a pivotal role in post-transcriptional regulation for rna metabolism and rna function in gene expression. we hypothesized and were going to identify that the regulatory function of pcbp1 is performed through different complexes of proteins that include pcbp1. | 2009 | 19727228 |
| artisanal alcohol production in mayan guatemala: chemical safety evaluation with special regard to acetaldehyde contamination. | there is a lack of knowledge regarding the composition, production, distribution, and consumption of artisanal alcohol, particularly in the developing world. in nahualá, an indigenous mayan municipality located in highland guatemala, heavy alcohol consumption appears to have had a significant negative impact on health, a major role in cases of violence and domestic abuse, and a link to street habitation. cuxa, an artisanally, as well as commercially produced sugarcane alcohol, is widely consumed ... | 2009 | 19729189 |
| a non-canonical dna structure enables homologous recombination in various genetic systems. | homologous recombination, which is critical to genetic diversity, depends on homologous pairing (hp). hp is the switch from parental to recombinant base pairs, which requires expansion of inter-base pair spaces. this expansion unavoidably causes untwisting of the parental double-stranded dna. reca/rad51-catalyzed atp-dependent hp is extensively stimulated in vitro by negative supercoils, which compensates for untwisting. however, in vivo, double-stranded dna is relaxed by bound proteins and thus ... | 2009 | 19729448 |
| trypanosoma brucei atg8: structural insights into autophagic-like mechanisms in protozoa. | bioinformatic searches of genome databases revealed that the number of autophagy-related genes (atg) is considerably lower in trypanosomes than in higher eukaryotes and even in yeast. this raises the question of whether autophagy in this protozoan parasite is more primitive and represents a rudimentary paradigm due to its early branching off the evolutionary tree. we here present the crystal structure of tbatg8b. this molecule (mw 13.7 kda) belongs to the ubiquitin-like proteins showing the typi ... | 2009 | 19736525 |
| [study on interaction between sars-cov n and map19]. | severe acute respiratory syndrome coronavirus (sars-cov) is the etiological agent of sars, an emerging disease characterized by atypical pneumonia. using a yeast two-hybrid screen with the nucleocapsid (n)protein of sars-cov as a bait, the n protein was found to interact with map19, a non-enzymatic protein of masp(mannan-associated serine protease). the interaction between sars-cov n and map19 would be further tested in cells in this article. | 2009 | 19737459 |
| znrf1 interacts with tubulin and regulates cell morphogenesis. | the ubiquitin-proteasome system has been implicated in neuronal degeneration and regeneration. we demonstrated that overexpression of znrf1, which has been identified as a crucial molecule in nerve regeneration, causes morphological changes such as neurite-like elongation. molecular dissections showed that both the ring finger domain and zinc finger domain are required for morphological changes. furthermore, we identified beta-tubulin type 2 (tubb2) as a znrf1-binding protein by yeast two-hybrid ... | 2009 | 19737534 |
| the cellular phenotype of roberts syndrome fibroblasts as revealed by ectopic expression of esco2. | cohesion between sister chromatids is essential for faithful chromosome segregation. in budding yeast, the acetyltransferase eco1/ctf7 establishes cohesion during dna replication in s phase and in response to dna double strand breaks in g2/m phase. in humans two eco1 orthologs exist: esco1 and esco2. both proteins are required for proper sister chromatid cohesion, but their exact function is unclear at present. since esco2 has been identified as the gene defective in the rare autosomal recessive ... | 2009 | 19738907 |
| the human kinetochore ska1 complex facilitates microtubule depolymerization-coupled motility. | mitotic chromosome segregation requires that kinetochores attach to microtubule polymers and harness microtubule dynamics to drive chromosome movement. in budding yeast, the dam1 complex couples kinetochores with microtubule depolymerization. however, a metazoan homolog of the dam1 complex has not been identified. to identify proteins that play a corresponding role at the vertebrate kinetochore-microtubule interface, we isolated a three subunit human ska1 complex, including the previously unchar ... | 2009 | 19289083 |
| identification of the variant ala335val of med25 as responsible for cmt2b2: molecular data, functional studies of the sh3 recognition motif and correlation between wild-type med25 and pmp22 rna levels in cmt1a animal models. | charcot-marie-tooth (cmt) disease is a clinically and genetically heterogeneous disorder. all mendelian patterns of inheritance have been described. we identified a homozygous p.a335v mutation in the med25 gene in an extended costa rican family with autosomal recessively inherited charcot-marie-tooth neuropathy linked to the cmt2b2 locus in chromosome 19q13.3. med25, also known as arc92 and acid1, is a subunit of the human activator-recruited cofactor (arc), a family of large transcriptional coa ... | 2009 | 19290556 |
| interaction of a multi-domain adaptor protein, vinexin, with a rho-effector, rhotekin. | among various effector proteins for the rho small gtpase, the function(s) of rhotekin is almost unknown. we have identified a multi-domain adaptor protein, vinexin, as a binding partner for rhotekin, using yeast two-hybrid screening of a human heart library. rhotekin was found to associate with vinexin in vitro, in cos7 cells, and in brain tissues. the c-terminal pro-rich motif of rhotekin exhibited binding to the third sh3 domain of vinexin. the binding was little affected by rho but was inhibi ... | 2009 | 19294487 |
| role of p54 rna helicase activity and its c-terminal domain in translational repression, p-body localization and assembly. | the rna helicase p54 (ddx6, dhh1, me31b, cgh-1, rck) is a prototypic component of p-(rocessing) bodies in cells ranging from yeast to human. previously, we have shown that it is also a component of the large cytoplasmic polyadenylation element-binding protein translation repressor complex in xenopus oocytes and that when tethered to the 3' untranslated region, xp54 represses reporter mrna translation. here, we examine the role of the p54 helicase activity in translational repression and in p-bod ... | 2009 | 19297524 |
| mlh1 deficiency enhances tumor cell sensitivity to ganciclovir. | suicide gene therapy with herpes simplex virus thymidine kinase (hsv-tk) and ganciclovir (gcv) is notable for producing multi-log cytotoxicity in a unique pattern of delayed cytotoxicity in s-phase. as hydroxyurea, a ribonucleotide reductase inhibitor that activates mismatch repair, can increase sensitivity to gcv, we evaluated the role of mlh1, an essential mismatch repair protein, in gcv cytotoxicity. using hct116tk (hsv-tk-expressing) colon carcinoma cells that express or lack mlh1, cell-surv ... | 2009 | 19300472 |
| lipid-induced er stress: synergistic effects of sterols and saturated fatty acids. | stress within the endoplasmic reticulum (er) induces a coordinated response, namely the unfolded protein response (upr), devoted to helping the er cope with the accumulation of misfolded proteins. failure of the upr plays an important role in several human diseases. recent studies report that intracellular accumulation of saturated fatty acids (sfas) and cholesterol, seen in diseases of high incidence, such as obesity or atherosclerosis, results in er stress. in the present study, we evaluated t ... | 2009 | 19302420 |
| mutations of the epha2 receptor tyrosine kinase gene cause autosomal dominant congenital cataract. | congenital cataracts (ccs) are clinically and genetically heterogeneous. mutations in the same gene may lead to ccs differing in inheritance, morphology and severity. loci for autosomal dominant posterior polar cc and total cc have both been mapped to the chromosomal 1p36 region harboring the epha2 receptor tyrosine kinase gene. here, we report mutations of epha2 in three cc families from different ancestral groups. in a chinese family with posterior polar cc, we identified a missense mutation, ... | 2009 | 19306328 |
| cohesin, gene expression and development: lessons from drosophila. | the cohesin complex, discovered through its role in sister chromatid cohesion, also plays roles in gene expression and development in organisms from yeast to human. this review highlights what has been learned about the gene control and developmental functions of cohesin and the nipped-b (nipbl/scc2) cohesin loading factor in drosophila. the drosophila studies have provided unique insights into the aetiology of cornelia de lange syndrome (cdls), which is caused by mutations affecting sister chro ... | 2009 | 19308700 |
| heterochromatin and the cohesion of sister chromatids. | heterochromatin, once thought to be the useless junk of chromosomes, is now known to play significant roles in biology. underlying much of this newfound fame are links between the repressive chromatin structure and cohesin, the protein complex that mediates sister chromatid cohesion. heterochromatin-mediated recruitment and retention of cohesin to domains flanking centromeres promotes proper attachment of chromosomes to the mitotic and meiotic spindles. heterochromatin assembled periodically bet ... | 2009 | 19308703 |
| relevance of weak flavin binding in human d-amino acid oxidase. | in the brain, the human flavoprotein d-amino acid oxidase (hdaao) is involved in the degradation of the gliotransmitter d-serine, an important modulator of nmda-receptor-mediated neurotransmission; an increase in hdaao activity (that yields a decrease in d-serine concentration) was recently proposed to be among the molecular mechanisms leading to the onset of schizophrenia susceptibility. this human flavoenzyme is a stable homodimer (even in the apoprotein form) that distinguishes from known d-a ... | 2009 | 19309736 |
| overexpression of amyloid precursor protein increases copper content in hek293 cells. | amyloid precursor protein (app) is a transmembrane glycoprotein widely expressed in mammalian tissues and plays a central role in alzheimer's disease. however, its physiological function remains elusive. cu(2+) binding and reduction activities have been described in the extracellular app135-156 region, which might be relevant for cellular copper uptake and homeostasis. here, we assessed cu(2+) reduction and (64)cu uptake in two human hek293 cell lines overexpressing app. our results indicate tha ... | 2009 | 19318086 |
| integrating shotgun proteomics and mrna expression data to improve protein identification. | motivation: tandem mass spectrometry (ms/ms) offers fast and reliable characterization of complex protein mixtures, but suffers from low sensitivity in protein identification. in a typical shotgun proteomics experiment, it is assumed that all proteins are equally likely to be present. however, there is often other information available, e.g. the probability of a protein's presence is likely to correlate with its mrna concentration. results: we develop a bayesian score that estimates the posterio ... | 2009 | 19318424 |
| serologic assay to quantify human immunoglobulin g antibodies to the staphylococcus aureus iron surface determinant b antigen. | a direct binding luminex assay has been developed and validated for the detection of human immunoglobulin g (igg) antibodies to the staphylococcus aureus iron surface determinant b protein (isdb) in serum following natural infection or immunization with investigational saccharomyces cerevisiae-derived isdb-based vaccines. to ensure that isdb-specific igg antibodies are measured following immunization with s. cerevisiae-derived isdb, an escherichia coli-produced isdb antigen is used in the assay. ... | 2009 | 19321693 |
| the structure of atg4b-lc3 complex reveals the mechanism of lc3 processing and delipidation during autophagy. | atg8 is conjugated to phosphatidylethanolamine (pe) by ubiquitin-like conjugation reactions. atg8 has at least two functions in autophagy: membrane biogenesis and target recognition. regulation of pe conjugation and deconjugation of atg8 is crucial for these functions in which atg4 has a critical function by both processing atg8 precursors and deconjugating atg8-pe. here, we report the crystal structures of catalytically inert human atg4b (hsatg4b) in complex with processed and unprocessed forms ... | 2009 | 19322194 |
| significance of salivary adrenomedullin in the maintenance of oral health: stimulation of oral cell proliferation and antibacterial properties. | adrenomedullin (adm) promotes epithelial cell proliferation and antimicrobial activity in the gastrointestinal tract. since adm is also present in saliva, it was the objective of our study to investigate the role of salivary adm in the maintenance of oral health. we found mrna for adm and the specific receptors crlr-ramp2 and crlr-ramp3 expressed by the salivary glands and by oral keratinocytes. the hormone was detected in the glandular tissues by western blot, being slightly bigger than the syn ... | 2009 | 19323987 |
| tat-binding protein-1 (tbp-1), an atpase of 19s regulatory particles of the 26s proteasome, enhances androgen receptor function in cooperation with tbp-1-interacting protein/hop2. | the 26s proteasome, which degrades ubiquitinated proteins, appears to contribute to the cyclical loading of androgen receptor (ar) to androgen response elements of target gene promoters; however, the mechanism whereby the 26s proteasome modulates ar recruitment remains unknown. using yeast two-hybrid screening, we previously identified tat-binding protein-1 (tbp-1), an adenosine triphosphatase of 19s regulatory particles of the 26s proteasome, as a transcriptional coactivator of thyroid hormone ... | 2009 | 19325002 |
| mono-hydroxylated polychlorinated biphenyls are potent aryl hydrocarbon receptor ligands in recombinant yeast cells. | the toxicities of polychlorinated biphenyls (pcbs) are thought to be mediated mainly by the aryl hydrocarbon receptor (ahr). however, little is known about changes to ahr-mediated effects caused by metabolic conversion of pcbs. to investigate whether hydroxylation affects the affinity of pcbs for the ahr, we measured the ahr agonistic activity of mono-hydroxylated pcbs (mono-oh-pcbs) and their non-hydroxylated analogs (pcbs) using yeast cells transduced with the human ahr and its response pathwa ... | 2009 | 19327395 |
| mst2- and furry-mediated activation of ndr1 kinase is critical for precise alignment of mitotic chromosomes. | the precise alignment of chromosomes on the metaphase plate prior to the onset of anaphase is essential for ensuring equal segregation of sister chromatids into two daughter cells, and defects in this process potentially cause chromosome instability and tumor progression [1-3]. ndr1 is an evolutionarily conserved serine/threonine kinase whose activity is regulated by mst kinases, furry (fry), and mob [4]. although the ndr1 signaling pathway is implicated in cell division and morphogenesis in yea ... | 2009 | 19327996 |
| structural and functional interaction between the human dna repair proteins dna ligase iv and xrcc4. | nonhomologous end-joining represents the major pathway used by human cells to repair dna double-strand breaks. it relies on the xrcc4/dna ligase iv complex to reseal dna strands. here we report the high-resolution crystal structure of human xrcc4 bound to the carboxy-terminal tandem brct repeat of dna ligase iv. the structure differs from the homologous saccharomyces cerevisiae complex and reveals an extensive dna ligase iv binding interface formed by a helix-loop-helix structure within the inte ... | 2009 | 19332554 |
| loss of mannosylphosphate from candida albicans cell wall proteins results in enhanced resistance to the inhibitory effect of a cationic antimicrobial peptide via reduced peptide binding to the cell surface. | the outermost layer of the candida albicans cell wall is enriched with mannosylated glycoproteins. we have used a range of isogenic glycosylation mutants of c. albicans, which are defective to varying degrees in cell wall protein mannosylation, to investigate the role of the outermost layer of the yeast cell wall in mediating the fungicidal action of the cationic, alpha-helical antimicrobial peptide dermaseptin s3(1-16) [dss3(1-16)]. the degree of phosphomannan loss, and concomitant reduction in ... | 2009 | 19332808 |
| the rnase rny1p cleaves trnas and promotes cell death during oxidative stress in saccharomyces cerevisiae. | the cellular response to stress conditions involves a decision between survival or cell death when damage is severe. a conserved stress response in eukaryotes involves endonucleolytic cleavage of transfer rnas (trnas). the mechanism and significance of such trna cleavage is unknown. we show that in yeast, trnas are cleaved by the rnase t2 family member rny1p, which is released from the vacuole into the cytosol during oxidative stress. rny1p modulates yeast cell survival during oxidative stress i ... | 2009 | 19332891 |
| a top-down approach to infer and compare domain-domain interactions across eight model organisms. | knowledge of specific domain-domain interactions (ddis) is essential to understand the functional significance of protein interaction networks. despite the availability of an enormous amount of data on protein-protein interactions (ppis), very little is known about specific ddis occurring in them. here, we present a top-down approach to accurately infer functionally relevant ddis from ppi data. we created a comprehensive, non-redundant dataset of 209,165 experimentally-derived ppis by combining ... | 2009 | 19333396 |
| selective vulnerability in alzheimer's disease: amyloid precursor protein and p75(ntr) interaction. | selective neuronal vulnerability in neurodegenerative diseases is poorly understood. in alzheimer's disease, the basal forebrain cholinergic neurons are selectively vulnerable, putatively because of their expression of the cell death mediator p75(ntr) (the common neurotrophin receptor), and its interaction with proapoptotic ligands pro-nerve growth factor and amyloid-beta peptide. however, the relation between amyloid precursor protein (app) and p75(ntr) has not been described previously. | 2009 | 19334058 |
| [transcriptional regulation of the human gene coding for proline-rich nuclear receptor coactivator (pnrc) by regulatory factor x (rfx1)]. | pnrc (proline-rich nuclear receptor coactivator) is a novel coactivator for multiple nuclear receptors. pnrc was previously identified using bovine sf-1 (steroidogenic factor 1) as the bait in a yeast two-hybrid screening of a human mammary gland cdna expression library. to understand the molecular mechanisms that regulate the expression of human pnrc gene, in this study, functional analysis of the 5' flanking region of the human pnrc gene revealed that the -123/+27 region is the minimal promote ... | 2009 | 19334528 |
| codon usage bias is correlated with gene expression levels in the fission yeast schizosaccharomyces pombe. | usage of synonymous codons represents a characteristic pattern of preference in each organism. it has been inferred that such bias of codon usage has evolved as a result of adaptation for efficient synthesis of proteins. here we examined synonymous codon usage in genes of the fission yeast schizosaccharomyces pombe, and compared codon usage bias with expression levels of the gene. in this organism, synonymous codon usage bias was correlated with expression levels of the gene; the bias was most o ... | 2009 | 19335619 |
| a novel protein phosphorylation pathway involved in osmotic-stress response in tobacco plants. | osmotic stress is one of the severest environmental pressures for plants, commonly occurring under natural growing condition due to drought, salinity, cold and wounding. plants sensitively respond to these stresses by activating a set of genes, which encode proteins necessary to overcome the crises. we screened such genes from tobacco plants, and identified a particular clone, which encoded a 45 kda protein kinase belonging to the plant receptor-like cytoplasmic protein kinase class-vii, nak (no ... | 2009 | 19340923 |
| vaginal microbiota and the use of probiotics. | the human vagina is inhabited by a range of microbes from a pool of over 50 species. lactobacilli are the most common, particularly in healthy women. the microbiota can change composition rapidly, for reasons that are not fully clear. this can lead to infection or to a state in which organisms with pathogenic potential coexist with other commensals. the most common urogenital infection in premenopausal women is bacterial vaginosis (bv), a condition characterized by a depletion of lactobacilli po ... | 2009 | 19343185 |
| fe65 binds teashirt, inhibiting expression of the primate-specific caspase-4. | the alzheimer disease (ad) amyloid protein precursor (app) can bind the fe65 adaptor protein and this complex can regulate gene expression. we carried out yeast two-hybrid studies with a ptb domain of fe65, focusing on those genes that might be involved in nuclear signaling, and identified and validated teashirt proteins as fe65 interacting proteins in neurons. using reporter systems, we observed that fe65 could simultaneously recruit set, a component of the inhibitor of acetyl transferase, and ... | 2009 | 19343227 |
| evaluation of biodegradation of nonylphenol ethoxylate and lignin by combining toxicity assessment and chemical characterization. | the aerobic biodegradation of commercial nonylphenol ethoxylate (npe) mixture and alkali lignin was studied using the oecd headspace test accompanied by the simultaneous measurement of ecotoxicity directly from the biodegradation liquors and by the follow-up of the chemical composition of the studied chemicals. npe degradation was dependent on the inoculum source: approximately 40% of npe was mineralized into co(2) during the 4-week experiment when inoculum from helsinki city wastewater treatmen ... | 2009 | 19344930 |
| trim32 is an e3 ubiquitin ligase for dysbindin. | mutations in the gene encoding tripartite motif protein 32 (trim32) cause two seemingly diverse diseases: limb-girdle muscular dystrophy type 2h (lgmd2h) or sarcotubular myopathy (stm) and bardet-biedl syndrome type 11(bbs11). although trim32 is involved in protein ubiquitination, its substrates and the molecular consequences of disease-causing mutations are poorly understood. in this paper, we show that trim32 is a widely expressed ubiquitin ligase that is localized to the z-line in skeletal mu ... | 2009 | 19349376 |
| medium optimization for keratinase production in hair substrate by a new bacillus subtilis kd-n2 using response surface methodology. | culture medium for keratinase production from hair substrate by a new bacillus subtilis strain, kd-n2, was optimized. effects of culture conditions on keratinase production were tested, and optimal results were obtained with 10% inocula (v/v), 16 g/l hair substrate, an initial ph value of 6.5 and a culture volume of 20 ml. several carbon sources (sucrose, cornflour) and nitrogen sources (yeast extract, tryptone and peptone) had positive effects on keratinase production, with sucrose giving optim ... | 2009 | 19350297 |
| global histone analysis by mass spectrometry reveals a high content of acetylated lysine residues in the malaria parasite plasmodium falciparum. | post-translational modifications (ptms) of histone tails play a key role in epigenetic regulation of gene expression in a range of organisms from yeast to human; however, little is known about histone proteins from the parasite that causes malaria in humans, plasmodium falciparum. we characterized p. falciparum histone ptms using advanced mass spectrometry driven proteomics. acid-extracted proteins were resolved in sds-page, in-gel trypsin digested, and analyzed by reversed-phase lc-ms/ms. throu ... | 2009 | 19351122 |
| in situ proteolysis to generate crystals for structure determination: an update. | for every 100 purified proteins that enter crystallization trials, an average of 30 form crystals, and among these only 13-15 crystallize in a form that enables structure determination. in 2007, dong et al reported that the addition of trace amounts of protease to crystallization trials--in situ proteolysis--significantly increased the number of proteins in a given set that produce diffraction quality crystals. 69 proteins that had previously resisted structure determination were subjected to cr ... | 2009 | 19352432 |
| o-demethylation of dextromethorphan using microbial cultures. | the potential of various microbes to metabolize the dextromethorphan (dxm) , a cyp 2d6 substrate was studied to investigate similarities between microbial and mammalian metabolism. eight microbes were screened for their ability to metabolize dxm in a manner comparable to humans with a view to develop alternative systems to study human drug metabolism. the major metabolite of dxm produced by saccharomyces cerevisiae (ncim 3090) was characterized by hplc and liquid chromatography mass spectrometry ... | 2009 | 19356111 |
| selenoprotein gene expression in thyroid and pituitary of young pigs is not affected by dietary selenium deficiency or excess. | expression and function of selenoproteins in endocrine tissues remain unclear, largely due to limited sample availability. pigs have a greater metabolic similarity and tissue size than rodents as a model of humans for that purpose. we conducted 2 experiments: 1) we cloned 5 novel porcine selenoprotein genes; and 2) we compared the effects of dietary selenium (se) on mrna levels of 12 selenoproteins, activities of 4 antioxidant enzymes, and se concentrations in testis, thyroid, and pituitary with ... | 2009 | 19357213 |
| a genome-wide association study identifies a novel locus on chromosome 18q12.2 influencing white cell telomere length. | telomere length is a predictor for a number of common age related diseases and is a heritable trait. | 2009 | 19359265 |
| the c-terminal active site cysteine of escherichia coli glutaredoxin 1 determines the glutathione specificity of the second step of peptide deglutathionylation. | glutaredoxins are oxidoreductases specialized in reducing glutathione-protein mixed disulfides. in the first step of deglutathionylation, glutaredoxins form a mixed disulfide with glutathione, releasing reduced peptide. the specificity of this reaction is based on the unusual amide linkage formed between the gamma-carboxylate of the n-terminal glutamic acid and the alpha-amino group of the cysteine present in glutathione. in the second step of deglutathionylation, glutathione reduces the glutare ... | 2009 | 19361272 |
| the fic domain: regulation of cell signaling by adenylylation. | we show that the secreted antigen, ibpa, of the respiratory pathogen histophilus somni induces cytotoxicity in mammalian cells via its fic domains. fic domains are defined by a core hpfxxgngr motif and are conserved from bacteria to humans. we demonstrate that the fic domains of ibpa catalyze a unique reversible adenylylation event that uses atp to add an adenosine monophosphate (amp) moiety to a conserved tyrosine residue in the switch i region of rho gtpases. this modification requires the con ... | 2009 | 19362538 |
| transforming growth factor beta1 signaling via interaction with cell surface hyal-2 and recruitment of wwox/wox1. | transforming growth factor beta (tgf-beta) initiates multiple signal pathways and activates many downstream kinases. here, we determined that tgf-beta1 bound cell surface hyaluronidase hyal-2 on microvilli in type ii tgf-beta receptor-deficient hct116 cells, as determined by immunoelectron microscopy. this binding resulted in recruitment of proapoptotic wox1 (also named wwox or for) and formation of hyal-2.wox1 complexes for relocation to the nuclei. tgf-beta1 strengthened the binding of the cat ... | 2009 | 19366691 |
| mediator structural conservation and implications for the regulation mechanism. | mediator, the multisubunit complex that plays an essential role in the regulation of transcription initiation in all eukaryotes, was isolated using an affinity purification protocol that yields pure material suitable for structural analysis. conformational sorting of yeast mediator single-particle images characterized the inherent flexibility of the complex and made possible calculation of a cryo-em reconstruction. comparison of free and rna polymerase ii (rnapii) -associated yeast mediator reco ... | 2009 | 19368889 |
| identification of the neuroblastoma-amplified gene product as a component of the syntaxin 18 complex implicated in golgi-to-endoplasmic reticulum retrograde transport. | syntaxin 18, a soluble n-ethylmaleimide-sensitive factor (nsf) attachment protein receptor (snare) protein implicated in endoplasmic reticulum (er) membrane fusion, forms a complex with other snares (bnip1, p31, and sec22b) and several peripheral membrane components (sly1, zw10, and rint-1). in the present study, we showed that a peripheral membrane protein encoded by the neuroblastoma-amplified gene (nag) is a subunit of the syntaxin 18 complex. nag encodes a protein of 2371 amino acids, which ... | 2009 | 19369418 |
| specific and global regulation of mrna stability during osmotic stress in saccharomyces cerevisiae. | hyperosmotic stress yields reprogramming of gene expression in saccharomyces cerevisiae cells. most of this response is orchestrated by hog1, a stress-activated, mitogen-activated protein kinase (mapk) homologous to human p38. we investigated, on a genomic scale, the contribution of changes in transcription rates and mrna stabilities to the modulation of mrna amounts during the response to osmotic stress in wild-type and hog1 mutant cells. mild osmotic shock induces a broad mrna destabilization; ... | 2009 | 19369426 |
| enhanced genomic instabilities caused by deregulated microtubule dynamics and chromosome segregation: a perspective from genetic studies in mice. | aneuploidy is defined as numerical abnormalities of chromosomes and is frequently (>90%) present in solid tumors. in general, tumor cells become increasingly aneuploid with tumor progression. it has been proposed that enhanced genomic instability at least contributes significantly to, if not requires, tumor progression. two major modes for genomic instability are microsatellite instability (min) and chromosome instability (cin). min is associated with dna-level defects (e.g. mismatch repair defe ... | 2009 | 19372138 |
| soluble fusion expression and characterization of bioactive human beta-defensin 26 and 27. | this study reports the first successful recombinant expression of cationic antimicrobial peptides human beta-defensin-26 and human beta-defensin-27 in escherichia coli. hbd26 and hbd27 genes were synthesized through codon optimization, and each gene was then cloned into the expression vector pet32, which feature fusion protein thioredoxin at the n-terminal. the recombinant plasmids were then transformed into e. coli bl21 (de3) and cultured in mbl medium, which gave yields of hbd26 and hbd27 fusi ... | 2009 | 19373462 |
| requirements for e1a dependent transcription in the yeast saccharomyces cerevisiae. | the human adenovirus type 5 early region 1a (e1a) gene encodes proteins that are potent regulators of transcription. e1a does not bind dna directly, but is recruited to target promoters by the interaction with sequence specific dna binding proteins. in mammalian systems, e1a has been shown to contain two regions that can independently induce transcription when fused to a heterologous dna binding domain. when expressed in saccharomyces cerevisiae, each of these regions of e1a also acts as a stron ... | 2009 | 19374760 |
| the nucleotide binding dynamics of human msh2-msh3 are lesion dependent. | here we report that the human dna mismatch complex msh2-msh3 recognizes small loops by a mechanism different from that of msh2-msh6 for single-base mismatches. the subunits msh2 and msh3 can bind either adp or atp with similar affinities. upon binding to a dna loop, however, msh2-msh3 adopts a single 'nucleotide signature', in which the msh2 subunit is occupied by an adp molecule and the msh3 subunit is empty. subsequent atp binding and hydrolysis in the msh3 subunit promote adp-atp exchange in ... | 2009 | 19377479 |
| vma21 deficiency: a case of myocyte indigestion. | the vma21p protein in yeast is an essential assembly chaperone for the vacuolar atpase, the major proton pump of cellular membranes. in this issue, ramachandran et al. (2009) report that mutations in the gene encoding the human homolog vma21 cause the disease x-linked myopathy with excessive autophagy through an unexpected mechanism. | 2009 | 19379689 |
| bax activation by the bh3-only protein puma promotes cell dependence on antiapoptotic bcl-2 family members. | it is still unclear whether the bh3-only protein puma (p53 up-regulated modulator of apoptosis) can prime cells to death and render antiapoptotic bh3-binding bcl-2 homologues necessary for survival through its ability to directly interact with proapoptotic bax and activate it. in this study, we provide further evidence, using cell-free assays, that the bh3 domain of puma binds bax at an activation site that comprises the first helix of bax. we also show that, in yeast, puma interacts with bax an ... | 2009 | 19380879 |
| molecular cloning and functional characterization of two cyp619 cytochrome p450s involved in biosynthesis of patulin in aspergillus clavatus. | patulin is an acetate-derived tetraketide mycotoxin produced by several fungal species, especially aspergillus, penicillium and byssochlamys species. the health risks due to patulin consumption by humans have led many countries to regulate it in human food. previous studies have shown the involvement of cytochrome p450 monooxygenases in the hydroxylation of two precursors of patulin, m-cresol and m-hydroxybenzylalcohol. in the present study, two cytochrome p450 genes were identified in the genom ... | 2009 | 19383676 |
| shq1 is required prior to naf1 for assembly of h/aca small nucleolar and telomerase rnps. | assembly of h/aca rnps in yeast is aided by at least two accessory factors, naf1p and shq1p. although the function of naf1p and its human ortholog naf1 has been delineated in detail, that of shq1p and its putative human ortholog shq1 remains obscure. we demonstrate that shq1 indeed functions in the biogenesis of human h/aca rnps and we dissect its mechanism of action. like naf1, shq1 binds the major h/aca core protein and pseudouridine synthase nap57 (aka dyskerin) but precedes the assembly role ... | 2009 | 19383767 |
| dax-1 and sox6 molecular interplay results in an antagonistic effect in pre-mrna splicing. | dax-1 (dss-ahc critical region on the x chromosome-1) is a member of the nuclear hormone receptor superfamily that has an important role in steroidogenesis and gonadogenesis. to investigate the role of dax-1 in the testis, a yeast two-hybrid screen was performed and sox6, member of the sry box (sox) protein family, was cloned as candidate. the interaction was confirmed biochemically and expression of sox6 overlapped with that of dax-1 in the developing gonad, as well as in sertoli cells of the a ... | 2009 | 19384854 |
| mst4 and ezrin induce brush borders downstream of the lkb1/strad/mo25 polarization complex. | the human lkb1 kinase, encoded by the ortholog of the invertebrate par4 polarity gene, is mutated in peutz-jeghers cancer syndrome. lkb1 activity requires complex formation with the pseudokinase strad and the adaptor protein mo25. the complex can induce complete polarization in a single isolated intestinal epithelial cell. we describe an interaction between mo25alpha and a human serine/threonine kinase termed mst4. a homologous interaction occurs in the yeast schizosaccharomyces pombe in the con ... | 2009 | 19386264 |
| host responses to a versatile commensal: pamps and prrs interplay leading to tolerance or infection by candida albicans. | the molecular interactions between commensal microorganisms and their host are basically different from those triggered by pathogens since they involve tolerance. when the commensal is genetically equipped to become an opportunistic pathogen, as is the case with candida albicans, the picture becomes more complex. in this case, the balance between protection and invasion depends on host reactivity to altered microbial expression of ligands interacting with innate immune sensors. based on experime ... | 2009 | 19388906 |
| literature-based priors for gene regulatory networks. | motivation: the use of prior knowledge to improve gene regulatory network modelling has often been proposed. in this article we present the first research on the massive incorporation of prior knowledge from literature for bayesian network learning of gene networks. as the publication rate of scientific papers grows, updating online databases, which have been proposed as potential prior knowledge in past research, becomes increasingly challenging. the novelty of our approach lies in the use of g ... | 2009 | 19389730 |
| regulation of h3k4 trimethylation via cps40 (spp1) of compass is monoubiquitination independent: implication for a phe/tyr switch by the catalytic domain of set1. | the multiprotein complex set1/compass is the founding member of the histone h3 lysine 4 (h3k4) methyltransferases, whose human homologs include the mll and hset1 complexes. compass can mono-, di-, and trimethylate h3k4, but transitioning to di- and trimethylation requires prior h2b monoubiquitination followed by recruitment of the cps35 (swd2) subunit of compass. another subunit, cps40 (spp1), interacts directly with set1 and is only required for transitioning to trimethylation. to investigate h ... | 2009 | 19398585 |
| discovering structural cis-regulatory elements by modeling the behaviors of mrnas. | gene expression is regulated at each step from chromatin remodeling through translation and degradation. several known rna-binding regulatory proteins interact with specific rna secondary structures in addition to specific nucleotides. to provide a more comprehensive understanding of the regulation of gene expression, we developed an integrative computational approach that leverages functional genomics data and nucleotide sequences to discover rna secondary structure-defined cis-regulatory eleme ... | 2009 | 19401680 |
| a long-lasting, plasmin-activatable thrombin inhibitor aids clot lysis in vitro and does not promote bleeding in vivo. | the leech protein hirudin is a potent inhibitor of thrombin, but clinical use of recombinant hirudin is restricted by haemorrhagic risks, and complicated by hirudin's rapid clearance from the circulation. we previously employed albumin fusion to slow hirudin variant 3 (hv3) clearance. in this study, we hypothesized that reconfiguration of the chimera, appending human serum albumin (hsa) to the n-terminus of hv3, with an intervening plasmin cleavage site, would create a slowly cleared, plasmin-ac ... | 2009 | 19404540 |
| transcriptional activities of the pax6 gene eyeless regulate tissue specificity of ectopic eye formation in drosophila. | pax genes encode dna binding proteins that play pivotal roles in the determination of complex tissues. members of one subclass, pax6, function as selector genes and play key roles in the retinal development of all seeing animals. mutations within the pax6 homologs including fly eyeless, mouse small eye and human pax6 lead to severe retinal defects in their respective systems. in drosophila eyeless and twin of eyeless, play non-redundant roles in the developing retina. one particularly interestin ... | 2009 | 19406113 |
| frodos found: behold the cenp-a "ring" bearers. | cenp-a is a histone h3-like protein specific to centromeres that is essential for kinetochore formation and accurate chromosome segregation in eukaryotes. recent studies (dunleavy et al., 2009; foltz et al., 2009; perpelescu et al., 2009; pidoux et al., 2009; williams et al., 2009) analyze cenp-a binding proteins required for the recruitment of cenp-a to centromeres in humans and in fission yeast, bringing us closer to understanding how centromere identity is faithfully propagated. | 2009 | 19410539 |
| the secret message of heterochromatin: new insights into the mechanisms and function of centromeric and pericentric repeat sequence transcription. | in the fission yeast, s. pombe, small dsrna generated by rnai-dependent mechanisms are involved in the establishment and maintenance of heterochromatic regions. the existence of conserved features within the general organization of centromeric and pericentromeric repeats in yeast, mouse and human argues in favor of a conserved role for centromeric and pericentromeric-derived transcripts across these species. in support of this, evidence is accumulating that centromeric and pericentromeric sequen ... | 2009 | 19412885 |
| nutrient control of torc1, a cell-cycle regulator. | it is well established that the target of rapamycin (tor) protein kinase has pivotal roles in controlling cell functions (including protein synthesis, cell growth and cell proliferation) and is implicated in numerous human diseases. mammalian tor complex 1 (mtorc1) signalling is activated by hormones and growth factors, and is also stimulated by intracellular amino acids. recent research has provided important new insight into the poorly understood mechanism by which amino acids activate mtorc1 ... | 2009 | 19419870 |
| the dyslexia-associated protein kiaa0319 interacts with adaptor protein 2 and follows the classical clathrin-mediated endocytosis pathway. | recently, genetic studies have implicated kiaa0319 in developmental dyslexia, the most common of the childhood learning disorders. the first functional data indicated that the kiaa0319 protein is expressed on the plasma membrane and may be involved in neuronal migration. further analysis of the subcellular distribution of the overexpressed protein in mammalian cells indicates that kiaa0319 can colocalize with the early endosomal marker early endosome antigen 1 (eea1) in large intracellular vesic ... | 2009 | 19419997 |
| the yeast pif1 helicase prevents genomic instability caused by g-quadruplex-forming ceb1 sequences in vivo. | in budding yeast, the pif1 dna helicase is involved in the maintenance of both nuclear and mitochondrial genomes, but its role in these processes is still poorly understood. here, we provide evidence for a new pif1 function by demonstrating that its absence promotes genetic instability of alleles of the g-rich human minisatellite ceb1 inserted in the saccharomyces cerevisiae genome, but not of other tandem repeats. inactivation of other dna helicases, including sgs1, had no effect on ceb1 stabil ... | 2009 | 19424434 |
| expression of candida glabrata adhesins after exposure to chemical preservatives. | adherence of candida glabrata, an opportunistic yeast pathogen, to host cells is mediated in part by the epa family of adhesins, which are encoded largely at subtelomeric loci, where they are subject to transcriptional silencing. in analyzing the regulation of the subtelomeric epa6 gene, we found that its transcription is highly induced after exposure to methylparaben, propylparaben, or sorbate. these weak acid-related chemicals are widely used as antifungal preservatives in many consumer goods, ... | 2009 | 19426114 |
| assessing reliability of protein-protein interactions by integrative analysis of data in model organisms. | protein-protein interactions play vital roles in nearly all cellular processes and are involved in the construction of biological pathways such as metabolic and signal transduction pathways. although large-scale experiments have enabled the discovery of thousands of previously unknown linkages among proteins in many organisms, the high-throughput interaction data is often associated with high error rates. since protein interaction networks have been utilized in numerous biological inferences, th ... | 2009 | 19426453 |
| hset1: a novel approach for colon cancer therapy. | histone-methyl transferases (hmts) are key enzymes that post-translationally modify histones, and serve key role in gene expression, epigenetic regulation, and as determinants of survival in malignant cells. recent studies have shed light on the role of hset1 which is a key element of highly conserved multi-protein hmt complex that catalyze methylation of histone h3 lysine 4 (h3k4) regulating expression of specific proteins important for the malignant phenotype. to understand the importance of d ... | 2009 | 19426701 |
| n-acetyl cysteine protects against ionizing radiation-induced dna damage but not against cell killing in yeast and mammals. | ionizing radiation (ir) induces dna strand breaks leading to cell death or deleterious genome rearrangements. in the present study, we examined the role of n-acetyl-l-cysteine (nac), a clinically proven safe agent, for it's ability to protect against gamma-ray-induced dna strand breaks and/or dna deletions in yeast and mammals. in the yeast saccharomyces cerevisiae, dna deletions were scored by reversion to histidine prototrophy. human lymphoblastoid cells were examined for the frequency of gamm ... | 2009 | 19427509 |
| a novel recombinant human lactoferrin augments the bcg vaccine and protects alveolar integrity upon infection with mycobacterium tuberculosis in mice. | lactoferrin, an iron binding glycoprotein, possesses multiple immune modulatory activities, including the ability to promote antigen specific cell-mediated immunity. previous studies showed that adding bovine lactoferrin to the bcg vaccine (an attenuated strain of mycobacterium bovis bacillus calmette guerin) resulted in increased host protective responses upon subsequent challenge with virulent erdman mycobacterium tuberculosis (mtb) in mice. the studies outlined here investigate utility of a n ... | 2009 | 19428915 |
| a novel member of solute carrier family 25 (slc25a42) is a transporter of coenzyme a and adenosine 3',5'-diphosphate in human mitochondria. | mitochondrial carriers are a family of proteins that transport metabolites, nucleotides, and cofactors across the inner mitochondrial membrane thereby connecting cytosolic and matrix functions. the essential cofactor coenzyme a (coa) is synthesized outside the mitochondrial matrix and therefore must be transported into mitochondria where it is required for a number of fundamental processes. in this work we have functionally identified and characterized slc25a42, a novel human member of the mitoc ... | 2009 | 19429682 |
| decapping is preceded by 3' uridylation in a novel pathway of bulk mrna turnover. | both end structures of eukaryotic mrnas, namely the 5' cap and 3' poly(a) tail, are necessary for transcript stability, and loss of either is sufficient to stimulate decay. mrna turnover is classically thought to be initiated by deadenylation, as has been particularly well described in saccharomyces cerevisiae. here we describe two additional, parallel decay pathways in the fission yeast schizosaccharomyces pombe. first, in fission yeast mrna decapping is frequently independent of deadenylation. ... | 2009 | 19430462 |
| expression of hepatitis b surface antigen s domain in recombinant saccharomyces cerevisiae using gal1 promoter. | hepatitis b virus, a member of the hepadnavirus family, causes the acute and chronic diseases of the human liver. the s domain of hepatitis b virus surface antigen (shbsag) was expressed under the control of the galactose-inducible gal1 promoter in recombinant saccharomyces cerevisiae. batch fermentation of s. cerevisiae 2805/pdeltamfalpha-shbsag resulted in 4.92gl(-1) dry cell mass and 2.21mgl(-1) shbsag concentration. to improve the expression level of shbsag, the pdi1 gene encoding protein di ... | 2009 | 19433220 |
| [hev capsid protein interacts with cyp 2a6 and decreases its coumarin 7-hydroxylation activity]. | e2 is a recombinant hepatitis e virus capsid protein including its main antigenic determinants but lacking of the particle assembling domain. p239 was the c-terminal extending protein of e2 and could self-assemble to form virus like particles, which might serve as mimicry of virions both structurally and antigenically. we previously used yeast two-hybrid system to screen proteins interacting with e2 based on a human hepatocyte cdna library. one candidate was identified as the segment (aa388-437) ... | 2009 | 19437879 |
| prl-3 promotes epithelial mesenchymal transition by regulating cadherin directly. | prl-3 is a key gene associated with progression and metastasis of colorectal cancer. recently prl-3 was suggested to promote epithelial mesenchymal transition (emt) by downregulating e-cadherin expression. but the mechanisms of emt induced by prl-3 remain largely unknown. here we found that prl-3 could also promote emt in a colorectal cancer cell model sw480 with deficient e-cadherin expression in vivo and in vitro. prl-3 stable overexpression or knockdown sw480 cells were injected subcutaneousl ... | 2009 | 19440036 |
| hares and tortoises: the high- versus low-throughput proteomic race. | the analysis of the proteome can be undertaken with parallel, high-throughput techniques or those that analyze proteins in a serial, one-at-a-time manner. the former include 2-d gels and shotgun ms/ms; the latter includes libraries containing fusion proteins (gst, green fluorescent protein, tap-tag and others) that are engineered onto each protein in a proteome and then studied one by one. in this review, we explore the progress that these scientifically contrasting paradigms have made in measur ... | 2009 | 19441020 |
| the search for a human holliday junction resolvase. | four-way dna intermediates, known as holliday junctions, are formed during mitotic and meiotic recombination, and their efficient resolution is essential for proper chromosome segregation. bacteria, bacteriophages and archaea promote holliday junction resolution by the introduction of symmetrically related nicks across the junction, in reactions mediated by holliday junction resolvases. in 2008, after a search that lasted almost 20 years, a holliday junction resolvase was identified in humans. t ... | 2009 | 19442245 |