Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
growth inhibition of beauveria bassiana by bacteria isolated from the cuticular surface of the corn leafhopper, dalbulus maidis and the planthopper, delphacodes kuscheli, two important vectors of maize pathogens.abstract the phytosanitary importance of the corn leafhopper, dalbulus maidis (de long and wolcott) (hemiptera: cicadellidae) and the planthopper, delphacodes kuscheli fennah (hemiptera: delphacidae) lies in their ability to transmit phloem-associated plant pathogens, mainly viruses and mollicutes, and to cause considerable mechanical damage to corn plants during feeding and oviposition. fungi, particularly some members of the ascomycota, are likely candidates for biocontrol agents against these ...201121529147
efficacy of beauveria bassiana against the red imported fire ant, solenopsis invicta (hymenoptera: formicidae), in taiwan.background: combining biological control and chemical control could be used for controlling red imported fire ant (rifa), solenopsis invicta, more effectively. beauveria bassiana f256, a local strain from taiwan, was evaluated for its efficacy in the control of s. invicta under both laboratory and field conditions. results: the efficacies of different doses of b. bassiana (bb) using direct application and bait formulation methods were compared. the number of rifa workers killed by the direct app ...201121538803
development of metarhizium anisopliae and beauveria bassiana formulations for control of malaria mosquito larvae.the entomopathogenic fungi metarhizium anisopliae and beauveria bassiana have demonstrated effectiveness against anopheline larvae in the laboratory. however, utilising these fungi for the control of anopheline larvae under field conditions, relies on development of effective means of application as well as reducing their sensitivity to uv radiation, high temperatures and the inevitable contact with water. this study was conducted to develop formulations that facilitate the application of metarh ...201121342492
transmission of beauveria bassiana from male to female aedes aegypti mosquitoes.resistance to chemical insecticides plus high morbidity rates have lead to rising interest in fungi as candidates for biocontrol agents of mosquito vectors. in most studies fungal infections have been induced by exposure of mosquitoes to various surfaces treated with conidia. in the present study eight mexican strains of beauveria bassiana were assessed against aedes aegypti by direct exposure of females to 6 × 10(8) conidia ml (-1) on a filter paper, afterwards, the transmission of the least an ...201121352560
contribution of the gas1 gene of the entomopathogenic fungus beauveria bassiana, encoding a putative glycosylphosphatidylinositol-anchored beta-1,3-glucanosyltransferase, to conidial thermotolerance and virulence.beauveria bassiana is a mycoinsecticide alternative to chemicals for use in biological pest control. the fungus-insect interaction is also an emerging model system to examine unique aspects of the development, pathogenesis, and diversity of fungal lifestyles. the glycoside hydrolase 72 (gh72) family includes ß-1,3-glucanosyltransferases that are glycosylphosphatidylinositol (gpi)-anchored cell wall-modeling enzymes affecting fungal physiology. a putative b. bassiana gpi-anchored ß-1,3-glucanosyl ...201121357429
two hydrophobins are involved in fungal spore coat rodlet layer assembly and each play distinct roles in surface interactions, development and pathogenesis in the entomopathogenic fungus, beauveria bassiana.the entomogenous filamentous fungus, beauveria bassiana expresses two hydrophobin genes, hyd1 and hyd2, hypothesized to be involved in cell surface hydrophobicity, adhesion, virulence, and to constitute the protective spore coat structure known as the rodlet layer. targeted gene inactivation of hyd1 resulted in seemingly 'bald' conidia that contained significantly altered surface fascicles or bundles. these cells displayed decreased spore hydrophobicity, loss of water mediated dispersal, changes ...201121375591
inhibition of cytokine expression by a butanol extract from cordyceps bassiana.cordyceps species have been known since long as a multi-utility ethnomedicinal herbal in korea, china and japan. it has been reported to exhibit a number of properties such as anti-oxidative, anti-cancer, antiinflammatory, anti-diabetic, and anti-obesity effects. in a previously conducted study, we had demonstrated that the ethanol extract of cordyceps bassiana was able to suppress the production of interleukin (il)-12 and interferon (ifn)-gamma in macrophages and t lymphocytes. in this study, w ...201121391436
effects of combining microbial and chemical insecticides on mortality of the pecan weevil (coleoptera: curculionidae).the pecan weevil, curculio caryae (horn), is a key pest of pecan [carya illinoinensis (wangenh.) k. koch]. current control recommendations are based on chemical insecticide applications. microbial control agents such as the entomopathogenic nematode, steinernema carpocapsae (weiser) and the fungus beauveria bassiana (balsamo) vuillemin occur naturally in southeastern u.s. pecan orchards and have shown promise as alternative control agents for c. caryjae. conceivably, the chemical and microbial a ...201121404833
pathogenicity of metarhizium anisopliae (metch) sorok and beauveria bassiana (bals) vuill to adult phlebotomus duboscqi (neveu-lemaire) in the laboratory.biological control of sandflies using entomopathogenic fungi is a possible alternative to the expensive synthetic chemical control. it is potentially sustainable, less hazardous, and relatively inexpensive and merits further investigations. the objective of this study was to identify the most pathogenic fungal isolate(s) to sandflies in the laboratory.201121406735
survival of anopheline eggs and their susceptibility to infection with metarhizium anisopliae and beauveria bassiana under laboratory conditions.the viability of anopheles gambiae sensu stricto and anopheles arabiensis (diptera: culicidae) eggs over time and the ovicidal activity of beauveria bassiana (ascomycota: cordycipitaceae) and metarhizium anisopliae (ascomycota: clavicipitaceae) were investigated. eggs were incubated in soil or leaf litter for up to 12 weeks at 26°c and 75%, 86% or >98% relative humidity (rh). eggs were treated topically with m. anisopliae icipe-30 or b. bassiana i93-825 conidia in either water or oil-in-water fo ...201121424402
the nf-kb like factor dif has weaker effects on drosophila melanogaster immune defenses than previously thought.the toll pathway of drosophila melanogaster, when activated by the beauveria bassiana fungus, directs the expression of the drosomycin and metchnikowin antimicrobial peptide genes by inducing the translocation into the nucleus of the dif transcription factor. accordingly, dif mutants have been reported to have a lower resistance to fungi than control flies. however, as the longevity of non-infected dif flies has not been measured in previous studies, it could be that survival times after infecti ...201121424695
generation of two new macrolactones through sequential biotransformation of dihydroresorcylide.biotransformation is an effective method to generate new derivatives from natural products. combination of various enzymes or whole-cell biocatalysts creates new opportunities for natural product biosynthesis. dihydroresorcylide (1) is a phytotoxic macrolactone from acremonium aeae. it was first chlorinated at c-11 by an engineered escherichia coli bl21-codonplus (de3)-ril/pjz54 strain that overexpresses a fungal flavin-dependent halogenase, and subsequently glycosylated at 12-oh by beauveria ba ...201121425679
beauveria bassiana: synergistic effect with acaricides against the tick hyalomma anatolicum anatolicum (acari: ixodidae).owing to the need to combat the spread of chemical acaricide resistance in ticks, we evaluated the efficacy of a mixture of the entomopathogenic fungus beauveria bassiana at17 and acaricides for the control of hyalomma anatolicum anatolicum in china. a mixture of b. bassiana at17 at the concentration of 10(8)conidia/ml and the synthetic pyrethroid deltamethrin at concentrations of 2500, 250, 25, 5, 2.5, 0.5 and 0.25ppm was tested in vitro. the germination capability, vegetative growth, conidia p ...201121440547
occupational exposure to microorganisms used as biocontrol agents in plant production.exposure to bioaerosols containing fungi and bacteria may cause various deleterious respiratory health effects. fungi and bacteria are commercially produced and applied to the environment as microbiological pest control agents (mpcas). attention has been drawn towards the exposure and health risks due to the use of commercially important mpcas. as part of a risk evaluation this paper intends to review whether the exposure to mpcas (beauveria bassiana, verticillium lecanii, trichoderma harzianum, ...201121196399
entomopathogenic fungus as a biological control for an important vector of livestock disease: the culicoides biting midge.the recent outbreak of bluetongue virus in northern europe has led to an urgent need to identify control measures for the culicoides (diptera: ceratopogonidae) biting midges that transmit it. following successful use of the entomopathogenic fungus metarhizium anisopliae against larval stages of biting midge culicoides nubeculosus meigen, we investigated the efficacy of this strain and other fungi (beauveria bassiana, isaria fumosorosea and lecanicillium longisporum) as biocontrol agents against ...201121264343
expression and purification of a functionally active class i fungal hydrophobin from the entomopathogenic fungus beauveria bassiana in e. coli.hydrophobins represent a class of unique fungal proteins that have low molecular mass, are cysteine rich, and can self-assemble into two-dimensional arrays at water/air interfaces. these highly surface-active proteins are able to decrease the surface tension of water, thus allowing fungal structures to penetrate hydrophobic-hydrophilic barriers. due to their unusual biophysical properties, hydrophobins have been suggested for use in a wide range of biotechnological applications. here we describe ...201120640587
beauveria bassiana (ascomycota: hypocreales) as a management agent for free-living amblyomma americanum (acari: ixodidae) in ohio.unfed adult amblyomma americanum were exposed to the entomopathogenic fungus beauveria bassiana and placed in the field. ticks exposed to the fungus experienced higher mortality than those in the control group (p = 0.001). it is recommended that for inclusion in a tick management program, the fungus be applied in the late evening during the months march through june as this is when ticks are most active and conditions suitable for fungal activity occur.201120607363
characterization of beauveria bassiana neutral trehalase (bbnth1) and recognition of crucial stress-responsive elements to control its expression in response to multiple stresses.a neutral trehalase (nth1) of fungal entomopathogen beauveria bassiana was characterized for the first time as a 743-aa enzyme (84.4kda). to identify crucial stress-responsive elements (stres) to control the expression of the nth-coding gene (bbnth1) in response to different stresses, the full-length promoter (-2713bp) upstream of its open reading frame and three upstream-truncated fragments (-1912, -1060 and -560bp) were fused to the reporter gene egfp and then transformed into b. bassiana, res ...201120630729
[genetic diversity and population genetic structure of beauveria bassiana in northern china].a total of 622 isolates of beauveria bassiana collected from 13 provinces in northern china were divided into 13 provincial subpopulations, and 568 of the 622 isolates belonging to 8 insect orders and araneida, whose hosts could be indentified to order level, were divided into 9 host order subpopulations, with the genetic diversity and population genetic structure of the b. bassiana in northern china analyzed by using issr (inter-simple sequence repeat) technique. all the diversity indices showe ...201223431795
in vivo interactions of entomopathogenic fungi, beauveria spp. and metarhizium anisopliae with selected opportunistic soil fungi of sugarcane ecosystem.in the present study, the interactions of entomopathogenic fungi viz., beauveria bassiana, beauveria brongniartii and metarhizium anisopliae among themselves and three other opportunistic soil fungi from the sugarcane ecosystem namely, fusarium saachari, aspergillus sp. and penecillium sp. were assayed in vivo against galleria mellonella larvae. the tested fungi were co-applied on iv instar g. mellonella @ 1 x 10(7) ml(-1), in combinations of two, at the interval of 24 hrs either preceding or su ...201223359998
effects of reduced-risk pesticides and plant growth regulators on rove beetle (coleoptera: staphylinidae) adults.in many regions, pest management of greenhouse crops relies on the use of biological control agents; however, pesticides are also widely used, especially when dealing with multiple arthropod pests and attempting to maintain high esthetic standards. as such, there is interest in using biological control agents in conjunction with chemical control. however, the prospects of combining natural enemies and pesticides are not well known in many systems. the rove beetle, atheta coriaria (kraatz), is a ...201223356075
towards the development of an autocontamination trap system to manage populations of emerald ash borer (coleoptera: buprestidae) with the native entomopathogenic fungus, beauveria bassiana.emerald ash borer, agrilus planipennis fairmaire (coleoptera: buprestidae) is an invasive species from asia that was discovered in north america canada, in 2002. herein, we describe studies to develop an autocontamination trapping system to disseminate beauveria bassiana to control beetle populations. the standard trap for emerald ash borer in canada is a light green prism trap covered in an insect adhesive and baited with (z)-3-hexenol. we compared of green multifunnel traps, green intercept pa ...201223356056
a new dibenz[b,e]oxepine derivative, 1-hydroxy-10-methoxy-dibenz[b,e]oxepin-6,11-dione, from a marine-derived fungus, beauveria bassiana tpu942.1-hydroxy-10-methoxy-dibenz[b,e]oxepin-6,11-dione (1) was obtained from the culture broth of a marine-derived fungus, beauveria bassiana tpu942, isolated from a marine sponge collected at iriomote island in okinawa, together with two known compounds, chrysazin (2) and globosuxanthone a (3). the structure of 1 was elucidated on the basis of its spectroscopic data (hreims, 1d and 2d nmr experiments including ¹h--¹h cosy, hmqc and hmbc spectra). dibenz[b,e]oxepines are rare in nature, and only six ...201223342391
lack of phenotypic and evolutionary cross-resistance against parasitoids and pathogens in drosophila melanogaster.when organisms are attacked by multiple natural enemies, the evolution of a resistance mechanism to one natural enemy will be influenced by the degree of cross-resistance to another natural enemy. cross-resistance can be positive, when a resistance mechanism against one natural enemy also offers resistance to another; or negative, in the form of a trade-off, when an increase in resistance against one natural enemy results in a decrease in resistance against another. using drosophila melanogaster ...201223285247
different toxic and hormetic responses of bombus impatiens to beauveria bassiana, bacillus subtilis and spirotetramat.pollinator exposure to pesticides is a concern in agricultural systems that depend on pollinators for crop production. however, not all pesticides elicit toxic effects, and response to a pesticide will vary depending on dose and exposure route. the effects of biopesticide formulations of bacillus subtilis and beauveria bassiana and of the tetramic acid insecticide spirotetramat on the common eastern bumblebee, bombus impatiens, were evaluated. microcolonies of bees were exposed to field-rate or ...201223281229
olfactory response of phytoseiulus persimilis (acari: phytoseiidae) to untreated and beauveria bassiana-treated tetranychus urticae (acari: tetranychidae).determination of attraction and avoidance behavior of predators is important in concomitant use of multiple natural enemies to control a pest. the olfactory response of the predatory mite phytoseiulus persimilis was studied to odors related to tetranychus urticae adults infected by beauveria bassiana debi008 in 0, 24, 48 and 72 h intervals, both in absence and in presence of plants. in plant-present experiments, p. persimilis attraction was neither towards adults of t. urticae infected by 0.02 % ...201223271063
virulence of select entomopathogenic fungi to the brown marmorated stink bug, halyomorpha halys (stål) (heteroptera: pentatomidae).the brown marmorated stink bug. halyomorpha halys, (bmsb) is an exotic pest causing damage to fruits and vegetables. they also transmit diseases. integrated pest management strategies are lacking for this invasive. for the first time, information is presented on the virulence of several entomopathogenic fungi against bmsb. three beauveria bassiana and two metarhizium anispoliae isolates were bioassayed against adult bmsb. one b. bassiana isolate was the active ingredient in botanigard(®) which ...201222223199
evaluation of organically acceptable insecticides as stand-alone treatments and in rotation for managing yellowmargined leaf beetle, microtheca ochroloma (coleoptera: chrysomelidae), in organic crucifer production.background: yellowmargined leaf beetle, microtheca ochroloma, is the most damaging pest of organic crucifer crops in the southern united states. experiments were conducted over four growing seasons (2007-2010) in alabama to evaluate some botanical and microbial insecticides approved by the organic materials review institute (omri) against m. ochroloma in organically grown crucifer crops. insecticides evaluated included pyganic(®) (pyrethrum), aza-direct(®) (azadiractin), entrust(®) (spinosad) ...201222231993
evaluating the lethal and pre-lethal effects of a range of fungi against adult anopheles stephensi mosquitoes.insecticide resistance is seriously undermining efforts to eliminate malaria. in response, research on alternatives to the use of chemical insecticides against adult mosquito vectors has been increasing. fungal entomopathogens formulated as biopesticides have received much attention and have shown considerable potential. this research has necessarily focused on relatively few fungal isolates in order to 'prove concept'. further, most attention has been paid to examining fungal virulence (lethali ...201223126549
storage and persistence of a candidate fungal biopesticide for use against adult malaria vectors.new products aimed at augmenting or replacing chemical insecticides must have operational profiles that include both high efficacy in reducing vector numbers and/or blocking parasite transmission and be long lasting following application. research aimed at developing fungal spores as a biopesticide for vector control have shown considerable potential yet have not been directly assessed for their viability after long-term storage or following application in the field.201223098323
Diversity of Beauveria spp. isolates from pollen beetles Meligethes aeneus in Switzerland.Pollen beetles Meligethes aeneus were collected in oilseed rape fields at different sites in Switzerland in spring 2004-2005 and 32 isolates of the fungal genus Beauveria occurring as latent infections in the beetles were obtained and molecularly characterized. Three major clades, Beauveria bassiana sensu stricto (Clade A: n=13), Beauveriabrongniartii (Clade B: n=1) and Beauveria Clade C (n=18) were identified among the isolates based on sequences of the ITS region and the 5' end of EF1-a. B. ba ...201222008375
Improving UV resistance and virulence of Beauveria bassiana by genetic engineering with an exogenous tyrosinase gene.Insect pathogenic fungi like Beauveria bassiana have been developed as environmentally friendly biocontrol agents against arthropod pests. However, restrictive environmental factors, including solar ultraviolet (UV) radiation frequently lead to inconsistent field performance. To improve resistance to UV damage, we used Agrobacterium-mediated transformation to engineer B. bassiana with an exogenous tyrosinase gene. The results showed that the mitotically stable transformants produced larger amoun ...201222024554
genome-wide identification and immune response analysis of serine protease inhibitor genes in the silkworm, bombyx mori.in most insect species, a variety of serine protease inhibitors (spis) have been found in multiple tissues, including integument, gonad, salivary gland, and hemolymph, and are required for preventing unwanted proteolysis. these spis belong to different families and have distinct inhibitory mechanisms. herein, we predicted and characterized potential spi genes based on the genome sequences of silkworm, bombyx mori. as a result, a total of eighty spi genes were identified in b. mori. these spi gen ...201222348050
pyrokinin β-neuropeptide affects necrophoretic behavior in fire ants (s. invicta), and expression of β-np in a mycoinsecticide increases its virulence.fire ants are one of the world's most damaging invasive pests, with few means for their effective control. although ecologically friendly alternatives to chemical pesticides such as the insecticidal fungus beauveria bassiana have been suggested for the control of fire ant populations, their use has been limited due to the low virulence of the fungus and the length of time it takes to kill its target. we present a means of increasing the virulence of the fungal agent by expressing a fire ant neur ...201222238569
cyp52x1, representing new cytochrome p450 subfamily, displays fatty acid hydroxylase activity and contributes to virulence and growth on insect cuticular substrates in entomopathogenic fungus beauveria bassiana.infection of insects by the entomopathogenic fungus beauveria bassiana proceeds via attachment and penetration of the host cuticle. the outermost epicuticular layer or waxy layer of the insect represents a structure rich in lipids including abundant amounts of hydrocarbons and fatty acids. a member of a novel cytochrome p450 subfamily, cyp52x1, implicated in fatty acid assimilation by b. bassiana was characterized. b. bassiana targeted gene knockouts lacking bbcyp52x1 displayed reduced virulence ...201222393051
exploiting the behaviour of wild malaria vectors to achieve high infection with fungal biocontrol agents.control of mosquitoes that transmit malaria has been the mainstay in the fight against the disease, but alternative methods are required in view of emerging insecticide resistance. entomopathogenic fungi are candidate alternatives, but to date, few trials have translated the use of these agents to field-based evaluations of their actual impact on mosquito survival and malaria risk. mineral oil-formulations of the entomopathogenic fungi metarhizium anisopliae and beauveria bassiana were applied u ...201222449130
the mosquito melanization response is implicated in defense against the entomopathogenic fungus beauveria bassiana.mosquito immunity studies have focused mainly on characterizing immune effector mechanisms elicited against parasites, bacteria and more recently, viruses. however, those elicited against entomopathogenic fungi remain poorly understood, despite the ubiquitous nature of these microorganisms and their unique invasion route that bypasses the midgut epithelium, an important immune tissue and physical barrier. here, we used the malaria vector anopheles gambiae as a model to investigate the role of me ...201223166497
enhanced uv resistance and improved killing of malaria mosquitoes by photolyase transgenic entomopathogenic fungi.the low survival of microbial pest control agents exposed to uv is the major environmental factor limiting their effectiveness. using gene disruption we demonstrated that the insect pathogenic fungus metarhizium robertsii uses photolyases to remove uv-induced cyclobutane pyrimidine dimers (cpd) and pyrimidine (6-4) photoproducts [(6-4)pps] from its dna. however, this photorepair is insufficient to fix cpd lesions and prevent the loss of viability caused by seven hours of solar radiation. express ...201222912789
combined expression of antimicrobial genes (bbchit1 and ljamp2) in transgenic poplar enhances resistance to fungal pathogens.populus species are susceptible to infection by microbial pathogens that severely affect their growth and substantially decrease their economic value. in this study, two pathogenesis-related protein genes consisting of beauveria bassiana chitinase (bbchit1) and motherwort lipid-transfer protein (ljamp2) were introduced into chinese white poplar (populus tomentosa carr.) via agrobacterium-mediated transformation using the hygromycin (hyg) and neomycin phosphotransferase (nptii) genes as selectabl ...201222971569
composition and antimicrobial activity of fatty acids detected in the hygroscopic secretion collected from the secretory setae of larvae of the biting midge forcipomyia nigra (diptera: ceratopogonidae).the hygroscopic secretion produced by the secretory setae of terrestrial larvae of the biting midge forcipomyia nigra (winnertz) was analysed using gas chromatography coupled with mass spectrometry (gc-ms). the viscous secretion is stored at the top of each seta and absorbs water from moist air. gc-ms analyses (four independent tests) showed that the secretion contained 12 free fatty acids, the most abundant of which were oleic (18:1), palmitic (16:0), palmitoleic (16:1) and linoleic (18:2). oth ...201222781366
a novel protease inhibitor in bombyx mori is involved in defense against beauveria bassiana.entomopathogenic fungi, such as beauveria bassiana, penetrate the insect cuticle using a plethora of hydrolytic enzymes including cuticle-degrading proteases and chitinases, which are important virulence factors. the insect integument and hemolymph contains a relatively high concentration of protease inhibitors, which are closely involved with defense against pathogenic microorganisms. to elucidate the molecular mechanism underlying resistance against entomopathogenic fungi and to identify a new ...201222841512
additive contributions of two manganese-cored superoxide dismutases (mnsods) to antioxidation, uv tolerance and virulence of beauveria bassiana.the biocontrol potential of entomopathogenic fungi against arthropod pests depends on not only their virulence to target pests but tolerance to outdoor high temperature and solar uv irradiation. two beauveria bassiana superoxide dismutases (sods), bbsod2 and bbsod3, were characterized as cytosolic and mitochondrial manganese-cored isoenzymes (mnsods) dominating the total sod activity of the fungal entomopathogen under normal growth conditions. to probe their effects on the biocontrol potential o ...201222279579
susceptibility of two hymenopteran parasitoids of agrilus planipennis (coleoptera: buprestidae) to the entomopathogenic fungus beauveria bassiana (ascomycota: hypocreales).emerald ash borer (eab), agrilus planipennis fairmaire, native to asia, is killing ash trees (fraxinus spp.) across 15 states and southeastern canada. integrated pest management using biological control is the only viable long-term approach for controlling the spread of eab outside of host resistance. three hymenopteran parasitoids, spathius agrili yang, tetrastichus planipennisi yang, and oobius agrili zhang and huang were discovered attacking eab in china and were approved for release in the u ...201222245471
integrated management of european cherry fruit fly rhagoletis cerasi (l.): situation in switzerland and europe.the european cherry fruit fly, rhagoletis cerasi (l.) (diptera: tephritidae), is a highly destructive pest. the low tolerance for damaged fruit requires preventive insecticide treatments for a marketable crop. the phase-out of old insecticides threatens cherry production throughout the european union (eu). consequently, new management techniques and tools are needed. with the increasing number of dwarf tree orchards covered against rain to avoid fruit splitting, crop netting has become a viable, ...201226466721
evolution in biocontrol strains: insight from the harlequin ladybird harmonia axyridis.after being used as a biocontrol agent against aphids for decades without harmful consequences, the asian harlequin ladybird harmonia axyridis has suddenly become an invasive pest on a worldwide scale. we investigate the impact of captive breeding on several traits of this ladybird such as genetic diversity, fecundity, survival and pathogen resistance. we conducted an experiment in the laboratory to compare the fecundity and the susceptibility to the entomopathogenic fungus beauveria bassiana of ...201222949923
cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer fusarium proliferatum lf061.the ketoisovalerate reductase (ec 1.2.7.7 ) is required for the formation of beauvericin via the nonribosomal peptide synthetase biosynthetic pathway. it catalyzes the nadph-specific reduction of ketoisovaleric acid to hydroxyisovalerate. however, little is known about the bioinformatics' data about the 2-kiv reductase in fusarium. to date, heterologous production of the gene kivrfp from fusarium has not been achieved.201222916830
prevalence and diversity of viruses in the entomopathogenic fungus beauveria bassiana.viruses have been discovered in numerous fungal species, but unlike most known animal or plant viruses, they are rarely associated with deleterious effects on their hosts. the knowledge about viruses among entomopathogenic fungi is very limited, although their existence is suspected because of the presence of virus-like double-stranded rna (dsrna) in isolates of several species. beauveria bassiana is one of the most-studied species of entomopathogenic fungi; it has a cosmopolitan distribution an ...201223001673
entomopathogenic fungi as potential biocontrol agents of the ecto-parasitic mite, varroa destructor, and their effect on the immune response of honey bees (apis mellifera l.).three isolates of each of the entomopathogenic fungi, metarhizium anisopliae, beauveria bassiana and clonostachys rosea, were assessed for their pathogenicity to the honey bee parasitic mite, varroa destructor. the fungi were applied to varroa mites by immersing them in a spore solution, and then the inoculated mites were placed on honey bee brood inside capped cells. at 7 days post inoculation (dpi), the three fungi caused significant varroa mortality compared to non-inoculated mites. in brood ...201223000027
entomopathogenic fungi, metarhizium anisopliae and beauveria bassiana reduce the survival of xenopsylla brasiliensis larvae (siphonaptera: pulicidae).entomopathogenic fungi, particularly those belonging to the genera metarhizium and beauveria have shown great promise as arthropod vector control tools. these agents, however, have not been evaluated against flea vectors of plague.201222992264
microscopic analysis of cordyceps bassiana (anamorph stage: beauveria bassiana) stromata during artificial cultivation for commercial use. 201222990493
beauveria brongniartii on white grubs attacking sugarcane in south africa.beauveria brongniartii (saccardo) petch fungal infections were observed on the melolonthid hypopholis sommeri burmeister (coleoptera: scarabaeidae) at two sites (harden heights and canema) in the sugarcane producing area of the northern kwazulu-natal midlands of south africa. to initially identify the disease-causing fungus, 17 different fluorescently-labelled microsatellite pcr primers were used to target 78 isolates of beauveria spp. dna. microsatellite data resolved two distinct clusters of b ...201222982079
bioconversion of 7-hydroxyflavanone: isolation, characterization and bioactivity evaluation of twenty-one phase i and phase ii microbial metabolites.microbial metabolism of 7-hydroxyflavanone (1) with fungal culture cunninghamella blakesleeana (atcc 8688a), yielded flavanone 7-sulfate (2), 7,4'-dihydroxyflavanone (3), 6,7-dihydroxyflavanone (4), 6-hydroxyflavanone 7-sulfate (5), and 7-hydroxyflavanone 6-sulfate (6). mortierella zonata (atcc 13309) also transformed 1 to metabolites 2 and 3 as well as 4'-hydroxyflavanone 7-sulfate (7), flavan-4-cis-ol 7-sulfate (8), 2',4'-dihydroxychalcone (9), 7,8-dihydroxyflavanone (10), 8-hydroxyflavanone 7 ...201222976322
transformation of 8-prenylnaringenin by absidia coerulea and beauveria bassiana.beauveria bassiana am278 and absidia coerulea am93 converted 8-prenylnaringenin (1) into two glycoside derivatives (7-o-β-d-glucopyranoside) (2) and 7-o-β-d-4'''-o-methylglucopyranoside) (3) in high yields in processes conducted in saboraud medium. 8-prenylnaringenin 7-o-β-d-4'''-o-methylglucopyranoside (3) is a new compound. 8-prenylnaringenin-7-sulfate (4) was obtained in transformation of 1 by absidia coerulea am93 in a buffer. formation of conjugated products in this study proceeds in a mann ...201222975300
isolation of entomopathogenic fungi from northern thailand and their production in cereal grains.spore productivity in six entomopathogenic fungal strains isolated from insect cadavers at four locations in chiang mai province was evaluated in five cereal grains: white-rice, wheat, rye, corn and sorghum. according to sequence analysis of the internal transcribed spacer regions of these isolates, they were closely related to beauveria bassiana (2 isolates), metarhizium flavoviride (1 isolate), metarhizium anisopliae (1 isolate), paecilomyces lilacinus (1 isolate) and isaria tenuipes (1 isolat ...201222965474
specific 5-hydroxylation of piperlongumine by beauveria bassiana atcc 7159.a new hydroxylated derivative was efficiently prepared by transforming the natural anti-cancer product, piperlongumine, with beauveria bassiana atcc 7159. its structure was determined to be 5-hydroxylpiperlongumine on the basis of the spectroscopic data. the absolute configuration at c-5 was established as r by mosher's method.201222878194
evaluation of strains of metarhizium anisopliae and beauveria bassiana against spodoptera litura on the basis of their virulence, germination rate, conidia production, radial growth and enzyme activity.ten strains of the entomopathogenic fungi metarhizium anisopliae and beauveria bassiana were evaluated to find the most effective strain for optimization studies. the first criterion tested for strain selection was the mortality (> 50%) of spodoptera litura larvae after inoculation of the fungus for 4 days. results on several bioassays revealed that b. bassiana bnbcrc showed the most virulence on mortality s. litura larvae (80% mortality). b. bassiana bnbcrc also showed the highest germination r ...201222870053
comparative evaluation of five beauveria isolates for housefly (musca domestica l.) control and growth optimization of selected strain.pathogenic potential of five native beauveria isolates was assessed against housefly adult and larvae in laboratory bioassays. beauveria isolate beauveria bassiana hq917687 showed highest virulence with 72.3 and 100 % mortality of larvae and adults of musca domestica, respectively. other beauveria isolates caused 36-52 % housefly larval mortality while the adult mortalities varied between 72 and 82 %. b. bassiana hq917687 also showed the fastest killing activity with lt(50) of 4 days (for larvae ...201222864861
susceptibility of different life stages of ornithodoros lahorensis to entomopathogenic fungi metarhizium anisopliae and beauveria bassiana.the use of chemicals for the control of arthropod pests can be problematic due to the potential for both environmental contamination and resistance development. as a result, there is an increasing interest in nonchemical alternatives, such as biocontrol by entomopathogenic fungi. in the present study, three strains of metarhizium anisopliae (v245, 3247, and 4456) and one strain of beauveria bassiana (lm 3.2) were evaluated under laboratory conditions for their virulence towards three life stages ...201222782474
genomic perspectives on the evolution of fungal entomopathogenicity in beauveria bassiana.the ascomycete fungus beauveria bassiana is a pathogen of hundreds of insect species and is commercially produced as an environmentally friendly mycoinsecticide. we sequenced the genome of b. bassiana and a phylogenomic analysis confirmed that ascomycete entomopathogenicity is polyphyletic, but also revealed convergent evolution to insect pathogenicity. we also found many species-specific virulence genes and gene family expansions and contractions that correlate with host ranges and pathogenic s ...201222761991
compatibility of the entomopathogenic fungus beauveria bassiana with flufenoxuron and azadirachtin against tetranychus urticae.laboratory studies were developed to evaluate the compatibility of flufenoxuron and azadirachtin with beauveria bassiana against tetranychus urticae larvae along with the required probit analysis of the involved chemicals on all of the life stages of this mite. flufenoxuron displayed parallel regression lines for the mortality of eggs, deutonymphs and adults. larvae and protonymphs were the most susceptible life stages. protonymphs were 35 times more sensitive than eggs and adults. azadirachtin ...201222744196
evaluation of entomopathogenic fungi and a nematode against the soil-dwelling stages of the crane fly tipula paludosa.larvae of the crane fly tipula paludosa (diptera: nematocera) are economically important pests of grasslands, nurseries and organically grown crops. this study was conducted to evaluate several entomopathogenic fungal strains, the entomopathogenic nematode heterorhabditis bacteriophora and the organophosphate insecticide dursban wg (75% chlorpyrifos) against larval stages of t. paludosa under laboratory and greenhouse conditions.201222740286
characterization and virulence of beauveria spp. recovered from emerald ash borer in southwestern ontario, canada.the emerald ash borer (eab), agrilus planipennis (coleoptera: buprestidae), is an invasive wood boring beetle that is decimating north america's ash trees (fraxinus spp.). to find effective and safe indigenous biocontrol agents to manage eab, we conducted a survey in 2008-2009 of entomopathogenic fungi (epf) infecting eab in five outbreak sites in southwestern ontario, canada. a total of 78 beauveria spp. isolates were retrieved from dead and mycosed eab cadavers residing in the phloem tissues o ...201222664171
susceptibility of different developmental stages of large pine weevil hylobius abietis (coleoptera: curculionidae) to entomopathogenic fungi and effect of fungal infection to adult weevils by formulation and application methods.the large pine weevil, hylobius abietis, is a major pest in european conifer forests causing millions of euros of damage annually. larvae develop in the stumps of recently felled trees; the emerging adults feed on the bark of seedlings and may kill them. this study investigated the susceptibility of different developmental stages of h. abietis to commercial and commercially viable isolates of entomopathogenic fungi, metarhizium and beauveria. all the developmental stages of h. abietis can be kil ...201222659253
chiral hplc analysis of donepezil, 5-o-desmethyl donepezil and 6-o-desmethyl donepezil in culture medium: application to fungal biotransformation studies.an high performance liquid chromatography (hplc) method for the enantioselective determination of donepezil (dpz), 5-o-desmethyl donepezil (5-odd), and 6-o-desmethyl donepezil (6-odd) in czapek culture medium to be applied to biotransformation studies with fungi is described for the first time. the hplc analysis was carried out using a chiralpak ad-h column with hexane/ethanol/methanol (75:20:5, v/v/v) plus 0.3 % triethylamine as mobile phase and uv detection at 270 nm. sample preparation was ca ...201222644152
the map kinase bbslt2 controls growth, conidiation, cell wall integrity, and virulence in the insect pathogenic fungus beauveria bassiana.entomopathogenic fungi, such as beauveria bassiana, are key environmental pathogens of insects that have been exploited for biological control of insect pests. mitogen-activated protein (map) kinases play crucial roles in regulating fungal development, growth, and pathogenicity, mediating responses to the environment. bbslt2, encoding for an slt2 family mapk, was isolated and characterized from b. bassiana. gene disruption of bbslt2 affected growth, caused a significant reduction in conidial pro ...201222587950
eicosanoid biosynthesis is activated via toll, but not imd signal pathway in response to fungal infection.phospholipase a(2) (pla(2)) catalyzes hydrolysis of phospholipids at sn-2 position and usually releases arachidonic acid, which is oxygenated into various eicosanoids that mediate innate immune responses in insects. pla(2) activities were measured in both immune-associated tissues of hemocyte and fat body in the beet armyworm, spodoptera exigua. upon challenge of an entomopathogenic fungus, beauveria bassiana, the pla(2)s were significantly activated in both hemocyte and fat body. the fungal inf ...201222569137
a preliminary evaluation of the potential of beauveria bassiana for bed bug control.residual biopesticide treatments of beauveria bassiana were tested against the bed bug cimex lectularius. an oil formulation of conidia was applied to different substrates. bed bugs were exposed for 1 h, transferred to an unsprayed environment and monitored for mortality. separate bioassays evaluated the effect of bed bug strain, sex, life stage, and exposure substrate on mortality. rapid mortality was observed in all bioassays, with bed bugs exposed to treated jersey knit cotton dying most rapi ...201222555012
in vitro chemoenzymatic and in vivo biocatalytic syntheses of new beauvericin analogues.new beauvericins have been synthesized using the nonribosomal peptide synthetase bbbeas from the entomopathogenic fungus beauveria bassiana. chemical diversity was generated by in vitro chemoenzymatic and in vivo whole cell biocatalytic syntheses using either a b. bassiana mutant or an e. coli strain expressing the bbbeas gene.201222547105
no evidence for immune priming in ants exposed to a fungal pathogen.there is accumulating evidence that invertebrates can acquire long-term protection against pathogens through immune priming. however, the range of pathogens eliciting immune priming and the specificity of the response remain unclear. here, we tested if the exposure to a natural fungal pathogen elicited immune priming in ants. we found no evidence for immune priming in formica selysi workers exposed to beauveria bassiana. the initial exposure of ants to the fungus did not alter their resistance i ...201222523588
cumulative impact of a clover cover crop on the persistence and efficacy of beauveria bassiana in suppressing the pecan weevil (coleoptera: curculionidae).the pecan weevil, curculio caryae (horn), is a key pest of pecan. endemic levels of the entomopathogenic fungus beauveria bassiana (balsamo) vuillemin can occur in pecan orchards and contribute to natural control of c. caryae. commercial formulations of the fungus can also be applied for suppression of c. caryae. we hypothesized that a clover cover crop enhances b. bassiana efficacy and persistence (e.g., by protecting the fungus against abiotic environmental stresses). the hypothesis was tested ...201222507002
a fungal homologue of neuronal calcium sensor-1, bbcsa1, regulates extracellular acidification and contributes to virulence in the entomopathogenic fungus beauveria bassiana.neuronal calcium sensor proteins and their homologues participate in transducing extracellular signals that affect intracellular ca(2+) levels, which in turn regulate enzyme activities, secretion, gene expression and other biological processes. the filamentous fungus beauveria bassiana is a broad-host-range pathogen of insects that acidifies the extracellular milieu during growth and pathogenesis towards target hosts. a collection of b. bassiana random insertion mutants were screened on ph indic ...201222504440
bioconversion of silybin to phase i and ii microbial metabolites with retained antioxidant activity.microbial transformation of silybin (1), the major flavonolignan of milk thistle (silybum marianum, asteraceae), resulted in the isolation of four metabolites. the structures of the isolated metabolites were determined by spectroscopic methods. one phase i metabolite was produced by beauveria bassiana and was characterized as 8-hydroxysilybin (2). three phase ii metabolites were produced by two cunninghamella species and were identified as 2,3-dehydrosilybin-3-o-β-d-glucoside (3), obtained from ...201222503365
tolerance and bioaccumulation of copper by the entomopathogen beauveria bassiana (bals.-criv.) vuill. exposed to various copper-based fungicides.this work evaluates for the first time the relationships between copper-tolerance, -solubilization and -bioaccumulation in the entomopathogen beauveria bassiana exposed to bordeaux mixture, copper oxychloride or copper hydroxide. bordeaux mixture was highly detrimental to fungus, by inhibiting the growth totally at the recommended dose (rd) and 2×rd. copper hydroxide and copper oxychloride were found to be less toxic, reducing fungus growth, sporulation and conidial germination in an average of ...201222484327
effect of oil-based formulations of acaripathogenic fungi to control rhipicephalus microplus ticks under laboratory conditions.the formulations of acaripathogenic fungi to control ticks have been widely studied. the present study evaluated the efficacy of oil-based formulations of metarhizium anisopliae sensu lato (s.l.), isolate ma 959, and beauveria bassiana, isolate bb 986, on different rhipicephalus microplus stages, comparing the efficacy between aqueous suspensions and 10, 15 and 20% mineral oil formulations. twelve groups were formed: one aqueous control group; three mineral oil control groups, at 10, 15 or 20%; ...201222480883
beauveria bassiana keratitis in bullous keratopathy: antifungal sensitivity testing and management.beauveria bassiana is a ubiquitous fungus available as an insecticide. in humans, it has limited virulence; to our knowledge, only 3 cases of invasive disease and 10 cases of keratitis have been documented.201222467587
variability in the production of extracellular enzymes by entomopathogenic fungi grown on different substrates.entomopathogenic fungi are important controllers of pest-insects populations in agricultural production systems and in natural environment. these fungi have enzymatic machinery which involve since the recognition and adherence of spores in their hosts culminating with infection and death of these insects. the main objective of this study was to analyzed extracellular enzyme production of the fungi strains beauveria bassiana, metarhizium anisopliae and paecilomyces sp when cultured on substrates. ...201224031896
transcriptome analysis of the entomopathogenic fungus beauveria bassiana grown on cuticular extracts of the coffee berry borer (hypothenemus hampei).the coffee berry borer (cbb; hypothenemus hampei) is a major pest of coffee responsible for significant crop losses worldwide. the entomopathogenic fungus beauveria bassiana represents a natural means of controlling this insect pest; however, little is known concerning the molecular determinants that contribute to the virulence of the fungus towards the cbb. in order to examine genes involved in insect virulence, two expressed sequence tag (est) libraries, representing germinating conidia and gr ...201222461485
occurrence of pathogenic fungi to amblyomma cajennense in a rural area of central brazil and their activities against vectors of rocky mountain spotted fever.two isolates of beauveria bassiana and one of purpureocillium lilacinum (=paecilomyces lilacinus) were found infecting amblyomma cajennense engorged females collected on horses (0.15% infection rate from a total of 1982 specimens) and another two isolates of p. lilacinum and one metarhizium anisopliae detected in soils (2.1% from 144 samples) collected in typical pasture habitats of this tick in central brazil from october 2009 to march 2011. fungi were isolated from soils with rhipicephalus san ...201222459109
selection of promising fungal biological control agent of the western flower thrips frankliniella occidentalis (pergande).larval stages of frankliniella occidentalis are known to be refractory to fungal infection compared with the adult stage. the objective of this study was to identify promising fungal isolate(s) for the control of larval stages of f. occidentalis.201222429122
identification of citrinin as the defence metabolite of penicillium corylophilum stressed with the antagonist fungus beauveria bassiana.penicillium corylophilum isolated from mosquitoes was cultivated in liquid media leading to the first reported identification of citrinin (1a) as one metabolic component of this fungus. the produced amount of 1a indicated this compound as the most abundant secondary metabolite of this fungus. stressing the culture of p. corylophilum with the presence of the antagonistic fungus beauveria bassiana increased in 65% the production of 1a. similar results were obtained with the presence of other fungi ...201222414191
selection of beauveria bassiana sensu lato and metarhizium anisopliae sensu lato isolates as microbial control agents against the boll weevil (anthonomus grandis) in argentina.the boll weevil (anthonomus grandis) is the main pest of cotton in the americas. the aim of this work was to evaluate isolates of the entomopathogenic fungi beauveria bassiana sensu lato and metarhizium anisopliae sensu lato virulent against a. grandis. screening was performed to evaluate the pathogenicity of 28 isolates of m. anisopliae s.l. and 66 isolates of b. bassiana s.l. against boll weevil adults. to select the isolates, lc(50) values of the most virulent isolates were calculated, and co ...201222326392
genetic variability of beauveria bassiana and a dna marker for environmental monitoring of a highly virulent isolate against cosmopolites sordidus.the banana weevil cosmopolites sordidus (germar) is one of a number of pests that attack banana crops. the use of the entomopathogenic fungus beauveria bassiana as a biological control agent for this pest may contribute towards reducing the application of chemical insecticides on banana crops. in this study, the genetic variability of a collection of brazilian isolates of b. bassiana was evaluated. samples were obtained from various geographic regions of brazil, and from different hosts of the c ...201224293712
[ change in the temperature preferences of beauveria bassiana sensu lato isolates in the latitude gradient of siberia and kazakhstan]. 201223156693
study of the rheological properties of a fermentation broth of the fungus beauveria bassiana in a bioreactor under different hydrodynamic conditions.fermentation with filamentous fungi in a bioreactor is a complex dynamic process that is affected by flow conditions and the evolution of the rheological properties of the medium. these properties are mainly affected by the biomass concentration and the morphology of the fungus. in this work, the rheological properties of a fermentation with the fungus beauveria bassiana under different hydrodynamic conditions were studied and the rheological behavior of this broth was simulated through a mixtur ...201223124340
expression and characteristic of the cu/zn superoxide dismutase gene from the insect parasitizing fungus cordyceps militaris.a cu/zn-superoxide dismutase (sod) gene was characterized from cordycepes militaris by gene cloning, heterogeneous expression and function analysis. this 154-aa sod (cmsod) was deduced from a 465-bp gene cloned, showing 72-95 % sequence identity to cu/zn-sods from other fungi. the deduced amino acid sequence of the cdna is highly similar to beauveria bassiana (95 %), isaria tenuipes (94 %) and claviceps purpurea (88 %), respectively. the sod gene of c. militaris spin 589 bp and consisted of two ...201223053936
could sterile males be used to vector a microbiological control agent? the case of rhynchophorus ferrugineus and beauveria bassiana.rhynchophorus ferrugineus (coleoptera, curculionidae) is the most threatening pest of palms worldwide. the potential of gamma-irradiated males to spread a pathogenic strain of the entomopathogenic fungus beauveria bassiana (ascomycota: clavicipitaceae) to control this pest was studied. first, the effects of gamma irradiation (15 and 25 gy) on the mating success and performance of adult males irradiated at age one day were studied in the laboratory. although male longevity decreased after irradia ...201323034248
use of uridine auxotrophy (ura3) for markerless transformation of the mycoinsecticide beauveria bassiana.genetic engineering offers a practical route for enhancing the insect biological control potential of entomopathogenic fungi such as beauveria bassiana. to date, however, such efforts have relied upon transformation protocols that utilize antibiotic or herbicidal resistance markers as selection agents for the introduction of genes into the fungus. in order to avoid the use of such markers for the development of field-usable fungal strains, a markerless transformation system based upon complement ...201323015100
comparison of volatile blends and nucleotide sequences of two beauveria bassiana isolates of different virulence and repellency towards the termite macrotermes michealseni.isolates of the fungus beauveria bassiana have different levels of virulence and repellency against the termite macrotermes michaelseni. in the present study, we compared the volatile profiles and gene sequences of two isolates of the fungus with different levels of virulence and repellence to the termite. gas chromatography-mass spectrometric analyses showed quantitative and qualitative differences in the composition of volatiles of the two isolates. the repellencies of synthetic blends of 10 p ...201323111683
sensitivity of the entomopathogenic fungus beauveria bassiana (bals.-criv.) vuill. to selected herbicides.the in vitro effect of six commonly used herbicides viz., amidosulfuron, dicamba, metribuzin, pyridate, s-metolachlor and tembotrione on mycelial growth of entomopathogenic fungus beauveriabassiana (atcc 74040) was investigated. mycelial growthrates at 15 and 25°c were evaluated on pda plates containing 100, 75, 50, 25, 12.5, 6.25 and 0% of the recommended application rate of each selected herbicide. the tested herbicides were classified in 4 scoring categories based on reduction of mycelial gro ...201323139237
neutral lipid composition changes in the fat bodies of engorged females rhipicephalus microplus ticks in response to fungal infections.the tick's fat body plays an essential role in energy storage and utilization. this study aimed to analyze the fat body neutral lipid composition in rhipicephalus microplus engorged females. in the first study (physiological profile of untreated ticks), the lipid analysis took place over the course of 4 days; the engorged females were incubated at optimal conditions and their fat bodies were dissected daily. fat body lipid analysis after fungal infection with metarhizium anisopliae sensu lato (s ...201323138474
chytrid mycoparasitism of entomophthoralean azygospores.mycoparasitism - when one fungus parasitizes another - has been reported to affect beauveria bassiana and mycorrhizal fungi in the field. however, mycoparasitism of any fungi in the order entomophthorales has never been reported before now. the majority of entomophthoralean species persist as resting spores (either zygospores or azygospores) in the environment and dormant entomophthoralean resting spores (whether formed as zygospores or azygospores) are thought to be especially well adapted for ...201324140499
evaluation of eye irritation by s-(-)-10,11-dihydroxyfarnesic acid methyl ester secreted by beauveria bassiana cs1029.the aim of this study was to investigate whether s-(-)-10,11-dihydroxyfarnesic acid methyl ester produced by cell subtype beauveria bassiana cs1029 causes acute toxicity when used for cosmetic purposes by performing an eye irritation test. new zealand white (nzw) rabbits were treated with a 100 mg/dose of s-(-)-10,11-dihydroxyfarnesic acid methyl ester according to standard procedure guidelines. no significant changes in terms of ocular lesions of the cornea, turbidity of the cornea, swelling of ...201324137288
the potential for controlling pangaeus bilineatus (heteroptera: cydnidae) using a combination of entomopathogens and an insecticide.the peanut burrower bug, pangaeus bilineatus (say), is an important pest of peanut (arachis hypogaea l.) in the southern united states. current control methods for this pest, which are based on the use of chemical insecticides, have not been successful. our objective was to determine if entomopathogens applied alone or in combination with a standard chemical insecticide would provide superior levels of p. bilineatus mortality compared with the standard chemical applied alone. specifically, we in ...201324224248
evaluation of beauveria bassiana for management of citrus thrips (thysanoptera: thripidae) in california blueberries.citrus thrips, scirtothrips citri (moulton), is a plant-feeding pest most widely recognized for causing damage to citrus and mango fruits. this insect has broadened its host range to become a significant pest of commercial blueberries grown in the san joaquin valley of california. we evaluated beauveria bassiana (balsamo) for control of citrus thrips in blueberries grown under two watering regimes (drip irrigation with and without overhead sprinklers) and using two fungal formulations (commercia ...201324224239
beauveria bassiana strains for biological control of cosmopolites sordidus (germ.) (coleoptera: curculionidae) in plantain.the objective of this study was to select strains of beauveria bassiana for controlling cosmopolites sordidus (germ.) in plantain farms (cv. terra) of the "recôncavo" and southern regions in the state of bahia, brazil. the virulence of 32 b. bassiana isolates against c. sordidus was determined under laboratory conditions. three isolates (cnpmf 407, cnpmf 218, and cnpmf 416) were selected for evaluation under field conditions in plantations located in the counties of mutuípe and wenceslau guimarã ...201324187659
cytokinesis-required cdc14 is a signaling hub of asexual development and multi-stress tolerance in beauveria bassiana.a dual-specificity, paralogue-free cdc14 phosphatase was located in the nuclei of beauveria bassiana (filamentous entomopathogen) and functionally characterized. inactivation of cdc14 caused defective cytokinesis due to multinucleate cells formed in δcdc14 and 89% decrease of blastospore production, followed by slower growth and a loss of ≥ 96% conidial yield under normal conditions. these defects coincided well with drastic down-regulation of 25 genes required for mitosis and conidiation. moreo ...201324169500
entomopathogenic fungi in cornfields and their potential to manage larval western corn rootworm diabrotica virgifera virgifera.entomopathogenic ascomycete fungi are ubiquitous in soil and on phylloplanes, and are important natural enemies of many soil-borne arthropods including larval western corn rootworm, diabrotica virgifera virgifera, which is a major pest of corn. we measured the prevalence of beauveria bassiana and metarhizium anisopliae sensu lato in ten cornfields in iowa, usa by baiting with larval insects. b. bassiana and m. anisopliae s.l. were present in 60% ± 6.3% and 55% ± 6.4% of soil samples, respectivel ...201324120889
synthesis and bioconversions of formestane.in an effort to generate new steroidal aromatase inhibitors, formestane (4-hydroxyandrost-4-ene-3,17-dione) (1) was biotransformed by rhizopus oryzae to yield the known 4β,5α-dihydroxyandrostane-3,17-dione as the major product (5) and bioconverted by beauveria bassiana to afford the known reduced 4,17β-dihydroxyandrost-4-en-3-one (6) and 3α,17β-dihydroxy-5β-androstan-4-one (7) and the new 4,11α,17β-trihydroxyandrost-4-en-3-one (8). all the metabolites showed more potent activities than their par ...201324074257
beauveria bassiana (ascomycota: hypocreales) wound dressing for the control of euzophera pinguis (lepidoptera: pyralidae).injury to olive tree trunks and branches because of biotic and abiotic factors, such as pruning and mechanical harvesting, attracts the olive pyralid moth euzophera pinguis haworth (lepidoptera: pyralidae). this moth has become increasingly important in the mediterranean region during recent years. the use of an entomopathogenic fungus for wound dressing for pest control is reported for the first time in this study. beauveria bassiana (ascomycota: hypocreales) strain eabb 08/04-ep was originally ...201324020271
more than a colour change: insect melanism, disease resistance and fecundity.a 'dark morph' melanic strain of the greater wax moth, galleria mellonella, was studied for its atypical, heightened resistance to infection with the entomopathogenic fungus, beauveria bassiana. we show that these insects exhibit multiple intraspecific immunity and physiological traits that distinguish them from a non-melanic, fungus-susceptible morph. the melanic and non-melanic morphs were geographical variants that had evolved different, independent defence strategies. melanic morphs exhibit ...201323698007
Refresh
firstprev
12345678910...
nextlast
Displaying items 701 - 800 of 1213