Publications

cupriavidus necator jmp134 rapidly reduces furfural with a zn-dependent alcohol dehydrogenase.	ethanol is a renewable biofuel, and it can be produced from lignocellulosic biomass. the biomass is usually converted to hydrolysates that consist of sugar and sugar derivatives, such as furfural. yeast ferments sugar to ethanol, but furfural higher than 3 mm is inhibitory. it can take several days for yeast cells to reduce furfural to non-inhibitory furfuryl alcohol before producing ethanol. bioreduction of furfural to furfuryl alcohol before fermentation may relieve yeast from furfural toxicit ...	2011	21526390
denitrification is a common feature among members of the genus bacillus.	although several gram-positive denitrifiers have been characterized in the past, there is still uncertainty about the occurrence of the denitrification trait among these bacteria. in an isolation campaign on luvisol soil, bacillus spp. were among the most abundant retrieved cultured denitrifiers next to members of rhizobiaceae family and genus cupriavidus. subsequent screening of 180 representatives of the genus bacillus (encompassing more than half of the current validly described species diver ...	2011	21530125
analyses of soluble and membrane proteomes of ralstonia eutropha h16 reveal major changes in the protein complement in adaptation to lithoautotrophy.	the soil-dwelling lithoautotrophic bacterium ralstonia eutropha h16 utilizes hydrogen as the key source of energy during aerobic growth on hydrogen and carbon dioxide. we examined the soluble and membrane protein complements of lithoautotrophically grown cells and compared them to the protein complements of cells grown organoheterotrophically on succinate. (14)n/(15)n-based inverse metabolic labeling in combination with gelc-ms led to the identification of 1452 proteins, 1174 of which could be q ...	2011	21561103
can the soil bacterium cupriavidus necator sense zno nanomaterials and aqueous zn(2+) differentially?	abstract synthetic metal oxide nanomaterials exert toxicity via two general mechanisms: release of free ions at concentrations which exert toxic effects upon the target cell, or specific surface-mediated physicochemical processes leading to the formation of hydroxyl free radicals and other reactive oxygen species which act to disrupt cell membranes and organelles. from a regulatory standpoint this presents a potential problem since it is not trivial to detect free metal ions in the presence of n ...	2011	21574811
removal of heavy metals using different polymer matrixes as support for bacterial immobilisation.	great attention is focused on the microbial treatment of metal contaminated environments. three bacterial strains, 1c2, 1zp4 and ec30, belonging to genera cupriavidus, sphingobacterium and alcaligenes, respectively, showing high tolerance to zn and cd, up to concentrations of 1000ppm, were isolated from a contaminated area in northern portugal. their contribution to zn and cd removal from aqueous streams using immobilised alginate, pectate and a synthetic cross-linked polymer was assessed. in mo ...	2011	21592655
assessing the potential for rhizoremediation of pcb contaminated soils in northern regions using native tree species.	rhizosphere bioremediation of polychlorinated biphenyls (pcbs) offers a potentially inexpensive approach to remediating contaminated soils that is particularly attractive in remote regions including the arctic. we assessed the abilities of two tree species native to alaska, salix alaxensis (felt-leaf willow) and picea glauca (white spruce), to promote microbial biodegradation of pcbs via the release of phytochemicals upon fine root death. crushed fine roots, biphenyl (pcb analogue) or salicylate ...	2011	21596420
[nife]- and [fes]-cofactors in the membrane-bound hydrogenase of ralstonia eutropha investigated by x-ray absorption spectroscopy: insights into o2-tolerant h2-cleavage.	molecular features that enable certain [nife] hydrogenases to catalyze hydrogen (h2) conversion in the presence of dioxygen (o2) were investigated in the present study. by means of x-ray absorption spectroscopy (xas) we compared the [nife] active site and fes clusters in the o2-tolerant membrane-bound hydrogenase (mbh) of ralstonia eutropha and the o2-sensitive periplasmic hydrogenase (ph) of desulfovibrio gigas. fe-xas indicated an unusual complement of iron-sulfur centers in the mbh, likely ba ...	2011	21618994
production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant corynebacterium glutamicum using propionate as a precursor.	lipopolysaccharides free p[3-hydroxybutyrate (3hb)-co-3-hydroxyvalerate (3hv)] production was achieved using recombinant corynebacterium glutamicum harboring polyhydroxyalkanoate (pha) biosynthetic genes from ralstonia eutropha. cells grown on glucose with feeding of propionate as a precursor of 3hv unit accumulated 8-47wt% of p(3hb-co-3hv). the 3hv fraction in the copolymer was varied from 0 to 28mol% depending on the propionate concentrations.	2011	20692303
phylogenetic relationships and diversity of β-rhizobia associated with mimosa species grown in sishuangbanna, china.	in order to investigate the genetic diversity of rhizobia associated with various exotic and invasive species in tropical mainland china, 116 bacterial isolates were obtained from mimosa root nodules collected from sishuangbanna and yuanjiang districts of yunnan province. isolated rhizobia were characterized by rflp analysis of 16s rrna genes, sds-page of whole-cell proteins and box-pcr. most of the isolated strains were identified as β-rhizobia belonging to diverse populations of burkholderia a ...	2011	20228206
A new DNA extraction method by controlled alkaline treatments from consolidated subsurface sediments.	Microbial communities that thrive in subterranean consolidated sediments are largely unknown owing to the difficulty of extracting DNA. As this difficulty is often attributed to DNA binding onto the silica-bearing sediment matrix, we developed a DNA extraction method for consolidated sediment from the deep subsurface in which silica minerals were dissolved by being heated under alkaline conditions. NaOH concentrations (0.07 and 0.33 N), incubation temperatures (65 and 94 °C) and incubation times ...	2012	22092362
burkholderia and cupriavidus spp. are the preferred symbionts of mimosa spp. in southern china.	rhizobia were isolated from invasive mimosa spp. (m. diplotricha and m. pudica) in dehong district of the province of yunnan in subtropical southern china. almost all of the 98 isolates were β-rhizobia in the genera burkholderia and cupriavidus. these strains were analyzed for their distribution characteristics together with strains from a previous study from sishuangbanna. the proportion of nodules containing each β-rhizobial genus varied between mimosa species, with cupriavidus being predomina ...	2012	22268711
new beta rhizobial strains able to efficiently nodulate parapiptadenia rigida (benth.) brenan.	among the leguminous trees natives to uruguay parapiptadenia rigida (angico), a mimosoideae legume, is one of the most promising species for agroforestry. like many other legumes, it is able to establish symbiotic associations with rhizobia and belongs to the group known as "nitrogen fixing trees", which are major components of agroforestry systems. information about rhizobial symbionts for this genus is scarce, and thus the aim of this work was to identify and characterize rhizobia associated w ...	2012	22226956
predicting metabolic pathways by sub-network extraction.	various methods result in groups of functionally related genes obtained from genomes (operons, regulons, syntheny groups, and phylogenetic profiles), transcriptomes (co-expression groups) and proteomes (modules of interacting proteins). when such groups contain two or more enzyme-coding genes, graph analysis methods can be applied to extract a metabolic pathway that interconnects them. we describe here the way to use the pathway extraction tool available on the neat web server ( ...	2012	22144151
Furfural reduction mechanism of a zinc-dependent alcohol dehydrogenase from Cupriavidus necator JMP134.	FurX is a tetrameric Zn-dependent alcohol dehydrogenase (ADH) from Cupriavidus necator JMP134. The enzyme rapidly reduces furfural with NADH as the reducing power. For the first time among characterized ADHs, the high-resolution structures of all reaction steps were obtained in a time-resolved manner, thereby illustrating the complete catalytic events of NADH-dependent reduction of furfural and the dynamic Zn(2+) coordination among Glu66, water, substrate and product. In the fully closed confor ...	2012	22081946