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polybrominated diphenyl ethers and their methoxylated analogs in mullet (mugil cephalus) and sea bass (dicentrarchus labrax) from bizerte lagoon, tunisia.concentrations of ten polybrominated diphenyl ethers (pbdes) and eight methoxylated polybrominated diphenyl ethers (meo-pbdes) in mullet (mugil cephalus) and sea bass (dicentrarchus labrax) collected from the bizerte lagoon and the mediterranean sea were investigated. to the best of our knowledge, this is the first report of these compounds in marine fishes from tunisia. the pbde mean concentrations in fish from bizerte lagoon were 45.3 and 96.2 ng g(-1) lw respectively in mullet and sea bass, w ...201121983038
bone morphogenetic protein 15 and growth differentiation factor 9 expression in the ovary of european sea bass (dicentrarchus labrax): cellular localization, developmental profiles, and response to unilateral ovariectomy.vertebrate oocytes actively contribute to follicle development by secreting a variety of growth factors, among which bone morphogenetic protein 15 (bmp15/bmp15) and growth differentiation factor 9 (gdf9/gdf9) have been paid particular attention. in the present study, we describe the cellular localization, the developmental profiles, and the response to unilateral ovariectomy (a procedure implying the surgical removal of one of the ovaries) of protein and mrna steady-state levels of bmp15 and gdf ...201121978589
modulation of cyp1a and genotoxic effects in european seabass (dicentrarchus labrax) exposed to weathered oil: a mesocosm study.the aim of this study was to assess medium-term toxicity of weathered oil on european seabass. a mesocosm system reproducing an oil spill at sea was applied. fish were collected after 48 h, 7, 30 and 60 days. cyp1a gene transcription, erod and udpgt activities, bile pahs metabolites and micronuclei frequency were investigated. a progressive disappearance of low molecular weight n-alkanes and pahs in the water of the mesocosm occurred during the experimentation. fishes exposed to oil displayed a ...201221963215
impact of ultraviolet-b radiation on planktonic fish larvae: alteration of the osmoregulatory function.coastal marine ecosystems are submitted to variations of several abiotic and biotic parameters, some of them related to global change. among them the ultraviolet-b (uv-b) radiation (uvbr: 280-320 nm) may strongly impact planktonic fish larvae. the consequences of an increase of uvbr on the osmoregulatory function of dicentrarchus labrax larvae have been investigated in this study. in young larvae of d. labrax, as in other teleosts, osmoregulation depends on tegumentary ion transporting cells, or ...201222018917
lernanthropus kroyeri (van beneden and hesse 1851) parasitic copepoda (siphonostomatoidae, lernanthropidae) of european cultured sea bass dicentrarchus labrax (linnaeus 1758) from corsica: ecological and morphological study.lernanthropus kroyeri (copepoda, siphonostomatoida: lernanthropidae) is a gill parasite found on the european sea bass dicentrarchus labrax. during a survey of sea bass of corsican fish farms, we studied the biology of this parasite under culture conditions. we first chose to conduct a scanning electron microscopic study to give additional information about the lifestyle of the parasite. our examinations made it possible to reveal some unreported superficial structures including details not desc ...201222179262
challenges in assessing the toxic effects of polycyclic aromatic hydrocarbons to marine organisms: a case study on the acute toxicity of pyrene to the european seabass (dicentrarchus labrax l.).the acute toxicity (96 h) of pyrene (py) to european seabass (dicentrachus labrax) juveniles assessed in a semi-static bioassay (ssb) with medium renewal at each 12h, and in a static bioassay (sb) without medium renewal was compared in laboratorial conditions (water py concentrations: 0.07-10 mg l(-1)). main findings in the ssb that assessed mainly the toxicity of py and its metabolites were: increased levels of bile py metabolites in good agreement with the profile of lipid peroxidation levels ...201222154001
incorporation of ascorbic acid and α-tocopherol to the extender media to enhance antioxidant system of cryopreserved sea bass sperm.despite the overwhelming application of sperm cryopreservation in aquaculture and broodstock management, its detrimental effects on sperm quality must be taken into account. imbalance of reactive oxygen species is considered one of the main triggers of cell damage after cryopreservation, because the spermatozoa antioxidant system is decimated during this process, mainly because the natural antioxidants present in seminal plasma diminish when sperm is diluted in extenders. it has been demonstrate ...201222153272
the retina is a target for gnrh-3 system in the european sea bass, dicentrarchus labrax.the european sea bass expresses three gnrh (gonadotrophin releasing hormone) forms that exert pleiotropic actions via several classes of receptors. the gnrh-1 form is responsible for the endogenous regulation of gonadotrophin release by the pituitary gland but the role of gnrh-2 and gnrh-3 remains unclear in fish. in a previous study performed in sea bass, we have provided evidence of direct links between the gnrh-2 cells and the pineal organ and demonstrated a functional role for gnrh-2 in the ...201222138555
characteristics of fads2 gene expression and putative promoter in european sea bass (dicentrarchus labrax): comparison with salmonid species and analysis of cpg methylation.marine fish species exhibit low capacity to biosynthesise highly unsaturated fatty acid (hufa) in comparison to strict freshwater and anadromous species. it is admitted that the delta(6) desaturase (fads2) is a key enzyme in the hufa biosynthetic pathway. we investigated by quantitative pcr the relative amounts of fads2 mrna in european sea bass (dicentrarchus labrax) in comparison with a salmonid species, the rainbow trout (oncorhynchus mykiss l.). the analysis of the expression data was perfor ...201222325717
responses of juvenile sea bass, dicentrarchus labrax, exposed to acute concentrations of crude oil, as assessed by molecular and physiological biomarkers.in the present study, juvenile sea bass were exposed for 48 and 96 h to an arabian light crude oil and their responses were assessed at the molecular and physiological levels. the aim of the study was therefore to assess (i) the short term effects of crude oil exposure by the measurement of several molecular biomarkers, (ii) the consequences of this short term exposure on fish health by using growth and condition indices measured after a decontamination period of 28 and 26 d in seawater. hydroca ...201222236592
use of near-infrared spectroscopy for fast fraud detection in seafood: application to the authentication of wild european sea bass (dicentrarchus labrax).the possibility of using near-infrared spectroscopy (nirs) for the authentication of wild european sea bass ( dicentrarchus labrax ) was investigated in this study. three different chemometric techniques to process the nir spectra were developed, and their ability to discriminate between wild and farmed sea bass samples was evaluated. one approach used spectral information to directly build the discrimination model in a latent variable space; the second approach first used wavelets to transform ...201222224758
a phylogeny of the temperate seabasses (moronidae) characterized by a translocation of the mt-nd6 gene.the entire mitochondrial genome of the striped bass morone saxatilis was sequenced together with the mitochondrial (mt) control regions of the white bass morone chrysops, white perch morone americana, yellow bass morone mississippiensis, spotted seabass dicentrarchus punctatus, european seabass dicentrarchus labrax and the japanese seabass lateolabrax japonicus. the resultant 17 580 base pair circular genome of m. saxatilis contains 38 genes (13 proteins, 23 transfer rnas and two ribosomal rnas) ...201222220893
oxidative stress, genotoxicity and histopathology biomarker responses in mullet (mugil cephalus) and sea bass (dicentrarchus labrax) liver from bizerte lagoon (tunisia).the aim of the study was to evaluate the impact of environmental contaminants on oxidative stress, genotoxic and histopathologic biomarkers in liver of mullet (mugil cephalus) and sea bass (dicentrarchus labrax) collected from a polluted coastal lagoon (bizerte lagoon) in comparison to a reference site (the mediterranean sea). antioxidant enzyme activities were lower in fish from the polluted site compared with fish from the reference site, suggesting deficiency of the antioxidant system to comp ...201222206722
diversity, molecular characterization and expression of t cell receptor γ in a teleost fish, the sea bass (dicentrarchus labrax, l).two lineages of t cells, expressing either the αβ t cell receptor (tr) or the γδ tr, exist in gnathostomes. the latter type of t cells account for 1-10 % of t cells in blood and up to 30 % in the small intestine. they may recognize unconventional antigens (phosphorylated microbial metabolites, lipid antigens) without the need of major histocompatibility class i (mh1) or class ii (mh2) presentation. in this work we have described cloning and structural characterization of tr -chain (trg) from the ...201223133531
acceleration performance of individual european sea bass dicentrarchus labrax measured with a sprint performance chamber: comparison with high-speed cinematography and correlates with ecological performance.locomotor performance can influence the ecological and evolutionary success of a species. for fish, favorable outcomes of predator-prey encounters are often presumably due to robust acceleration ability. although escape-response or "fast-start" studies utilizing high-speed cinematography are prevalent, little is known about the contribution of relative acceleration performance to ecological or evolutionary success in a species. this dearth of knowledge may be due to the time-consuming nature of ...201223099467
vitamin c enhances vitamin e status and reduces oxidative stress indicators in sea bass larvae fed high dha microdiets.docosahexaenoic acid (dha) is an essential fatty acid necessary for many biochemical, cellular and physiological functions in fish. however, high dietary levels of dha increase free radical injury in sea bass (dicentrarchus labrax) larvae muscle, even when vitamin e (α-tocopherol, α-toh) is increased. therefore, the inclusion of other nutrients with complementary antioxidant functions, such as vitamin c (ascorbic acid, vitc), could further contribute to prevent these lesions. the objective of th ...201223086553
molecular cloning, tissue distribution and daily expression of cry1 and cry2 clock genes in european seabass (dicentrarchus labrax).biological rhythms are driven by circadian oscillators, which are ultimately controlled by the cyclic expression of clock genes. cryptochromes (cry), blue light photoreceptors, belong to the negative elements of the transcriptional feedback loop into the molecular clock. this paper describes the cloning and characterization of two cryptochromes (cry1 and 2) in european seabass, which is considered an interesting chronobiology model due to its dual (diurnal/nocturnal) behavior. the cloned cdna fr ...201222841604
short-term exposure of the european sea bass dicentrarchus labrax to copper-based antifouling treated nets: copper bioavailability and biomarkers responses.we studied if the levels of copper released from antifouling treated nets used in finfish mariculture could affect the immune defense mechanism and/or induce oxidative stress in dicentrarchus labrax, after short term exposure in laboratory experiments. dissolved copper concentration released from the treated nets, copper bioavailability and a set of biomarkers responses were measured. biomarkers included hemoglobin concentration, activities of lysozyme, total complement, respiratory burst, gluta ...201222698372
differentiation of fresh and frozen/thawed fish, european sea bass (dicentrarchus labrax), gilthead seabream (sparus aurata), cod (gadus morhua) and salmon (salmo salar), using volatile compounds by spme/gc/ms.a simple method based on solid phase microextraction/gas chromatography/mass spectrometry (spme/gc/ms) was applied for studying the volatile profiles of whole fish samples of european sea bass (dicentrarchus labrax) and gilthead seabream (sparus aurata) and fillets of cod (gadus morhua) and salmon (salmo salar) during frozen storage in order to be able to differentiate a fresh product from one that has been frozen. analysis of volatile compounds was performed on these two product types, fresh an ...201222641336
rodlet cells development in the intestine of sea bass (dicentrarchus labrax).the enigmatic rodlet cells (rcs) are characterized by conspicuous inclusions named "rodlets". they were discovered over 100 years ago and were considered as parasites but shortly afterward interpreted as endogenous cells. the rcs have been described in different tissues of marine and freshwater teleosts, but their origin and function remain unknown. this work was designed to an ultrastructural study on rcs development and distribution in intestinal epithelium of dicentrarchus labrax. three diffe ...201222622998
hepatic glycogen synthesis in farmed european seabass (dicentrarchus labrax l.) is dominated by indirect pathway fluxes.hepatic glycogen synthesis fluxes from direct and indirect pathways were quantified in seabass by postmortem (2)h nmr analysis of plasma water (pw) and glycogen glucosyl (2)h enrichments from (2)h-enriched seawater. eighteen fish (28.0 ± 1.7 cm and 218.0 ± 43.0 g) were divided into three groups of 6 and studied over 24 days with transfer to 5% (2)h-seawater after day 21. over this period, one group was fed daily with fishmeal, a second group was fasted, and a third group was fasted for 21 days f ...201222561667
2d dige/ms to investigate the impact of slaughtering techniques on postmortem integrity of fish filet proteins.two-dimensional difference gel electrophoresis (2d dige) was applied to investigate the impact of slaughtering on the postmortem integrity of muscle tissue proteins in european sea bass (dicentrarchus labrax). three different slaughtering techniques were evaluated: asphyxia in air (aa), asphyxia in ice (ai), and spinal cord severance (scs). principal components analysis (pca) revealed a significant divergence of scs samples, whereas aa and ai samples, although grouped separately, were less diver ...201222561645
heritability of cortisol response to confinement stress in european sea bass dicentrarchus labrax.in fish, the most studied production traits in terms of heritability are body weight or growth, stress or disease resistance, while heritability of cortisol levels, widely used as a measure of response to stress, is less studied. in this study, we have estimated heritabilities of two growth traits (body weight and length) and of cortisol response to confinement stress in the european sea bass.201222520515
morphology of the european sea bass (dicentrarchus labrax) tongue.the european sea bass, a member of the moronidae family, is a food fish, considered one of the first models for the intensive breeding in salt water. it has nowadays an important and increasing presence in the international fishing markets. sea basses are carnivorous, feeding on little fishes and invertebrates. considering the important role of the tongue during the intraoral transport and the swallowing of food, scarce data are present in literature about its morphology. the aim of this study w ...201222505185
a monoclonal antibody for the cd45 receptor in the teleost fish dicentrarchus labrax.the cd45 tyrosine phosphatase plays an important role in regulating t lymphocyte activation in vertebrate species. in this study we describe some molecular and functional features of the cd45 receptor molecule from the european sea bass dicentrarchus labrax. following immunization with fixed sea bass thymocytes, we obtained a murine monoclonal antibody (mab) able to stain fish leucocytes both alive, by immunofluorescence of thymus and mucosal tissues, and fixed, by in situ immunohistochemistry o ...201222504161
sea bass sperm freezability is influenced by motility variables and membrane lipid composition but not by membrane integrity and lipid peroxidation.cryopreserved sperm quality depends on the characteristics of fresh sperm. thus, it is necessary to establish a group of variables to predict the cryopreservation potential of the fresh samples with the aim of optimizing resources. motility, viability, lipid peroxidation and lipid profile of european sea bass (dicentrarchus labrax) sperm were determined before and after cryopreservation to establish which variables more accurately predict the sperm cryopreservation potential in this species. cry ...201222503480
gene flow at major transitional areas in sea bass (dicentrarchus labrax) and the possible emergence of a hybrid swarm.the population genetic structure of sea bass (dicentrarchus labrax) along a transect from the atlantic ocean (ao) to the eastern mediterranean (em) sea differs from that of most other marine taxa in this area. three populations (ao, western mediterranean [wm], em) are recognized today, which were originally two allopatric populations. how two ancestral genetic units have evolved into three distinct units has not been addressed yet. therefore, to investigate mechanisms that lead to the emergence ...201223301173
kisspeptin and seasonal control of reproduction in male european sea bass (dicentrarchus labrax).in the present study, we developed and validated real-time quantitative rt-pcr assays for a suite of genes involved in the brain-pituitary gonadal axis in fish including kisspeptin genes and its receptor (kiss1, kiss2, kissr4) and gonadotropin-releasing hormone genes (sbgnrh, sgnrh, cgnrhii) in the brain, and gonadotropin genes (fshβ and lhβ) in the pituitary. sex steroid profiles (t and 11-kt) and gonadal development were also studied over a full annual reproductive cycle in adult male sea bass ...201223036731
trace elements in two marine fish species during estuarine residency: non-essential versus essential.trace element levels in fish are of particular interest, owing the potential risk to human health. in accordance, juveniles of dicentrarchus labrax and of liza aurata were sampled and arsenic, cadmium, chromium, selenium and zinc were determined in the muscle. the levels of trace elements in muscle demonstrated to be similar for both species and sites, with the exception of selenium levels at reference, which seemed to be higher in d. labrax. moreover, apart from arsenic levels in muscle, all el ...201223017951
effect of water temperature increase on ho-1 expression in european sea bass (dicentrarchus labrax l.) tissues.one of the most pertinent environmental factors influencing the marine organism life is temperature. it has been demonstrated that an increase of temperature is able to induce the synthesis of heat shock proteins (hsp). in this study we investigated the expression of ho-1 mrna, also referred to as hsp32, in different tissues of european sea bass (dicentrarchus labrax, l.) at several time points after increased temperature exposure (from 12degc to 30degc). our results showed that ho-1 was not exp ...201222992441
nodavirus infection induces a great innate cell-mediated cytotoxic activity in resistant, gilthead seabream, and susceptible, european sea bass, teleost fish.viral nervous necrosis (vnn) virus produces great mortalities in fish having susceptible and reservoir species between the most important marine aquaculture species. cell-mediated cytotoxicity (cmc) is considered, towards the interferon (ifn), the most important mechanism of the immune response to fight against viral infections but it has been very scarcely evaluated. we aimed to evaluate the effects of vnnv infection in the reservoir gilthead seabream (sparus aurata) and susceptible european se ...201222981914
mercury-induced chromosomal damage in wild fish (dicentrarchus labrax l.) reflecting aquatic contamination in contrasting seasons.ria de aveiro (mainly laranjo basin, portugal) has been subjected to mercury contamination from a chlor-alkali plant, currently presenting a well-described mercury gradient. this study aimed to assess mercury genotoxicity in this area by measuring the frequency of erythrocytic nuclear abnormalities (ena) in the european sea bass (dicentrarchus labrax), addressing the relation with total mercury concentration in the blood and the modulatory role of seasonal variables. fish were collected, in warm ...201222968208
quality of farmed and wild sea bass lipids studied by (1)h nmr: usefulness of this technique for differentiation on a qualitative and a quantitative basis.proton nuclear magnetic resonance ((1)h nmr) spectroscopy was employed to study the lipids of farmed and wild european sea bass (dicentrarchus labrax). this technique provided a great deal of detailed information on sea bass lipids composition and, once the spectra signals have been assigned, the simple observation of the spectra enables one to distinguish cultured from wild fish. this distinction is possible due to the presence in the former of high proportions of diunsaturated acyl groups, mai ...201222953897
characterisation and expression during sex differentiation of sox19 from the sea bass dicentrarchus labrax.the sox family of transcription factors are involved in a variety of developmental processes including sex determination and gonadal differentiation. sox19 is a particularly interesting member of this family that has been found only in fish, though mammals have a very diverged orthologue that is designated sox15 and assigned to a different sox family subgroup. here we describe the cloning and characterisation of sox19 from the european sea bass (dicentrarchus labrax), an important aquaculture sp ...201222940139
influence of pesticides on the ph regulatory enzyme, carbonic anhydrase, from european seabass liver and bovine erythrocytes.the objective of this study was to assess the inhibitory effects of six commonly used pesticides, cyhalothrin, cypermethrin, dichlorvos, methamidophos, chlorpyrifos and methylparathion, on the ph regulatory enzyme carbonic anhydrase (ca) of dicentrarchus labrax (european seabass) liver (dca) and bovine erythrocytes (bca). results of the study showed that the pesticides displayed quite variable inhibition profiles with k(i) values ranging from 0.376 to 26.164μm against dca, and from 1.174 to 53.2 ...201222561108
toxicity evaluation of diethylene glycol and its combined effects with produced waters of off-shore gas platforms in the adriatic sea (italy): bioassays with marine/estuarine species.diethylene glycol (deg) is commonly used to dehydrate natural gas in off-shore extraction plants and to prevent formation of gas hydrates. it may be released into the sea accidentally or in discharged produced waters (pws). pws samples from off-shore gas platforms in the adriatic sea (italy) have been used in this study. the objectives of the study were: a) to evaluate the toxicity of deg for marine organisms; b) to evaluate if a high deg content in pws may alter their toxicity; c) to verify whe ...201222245535
effects of garlic and ginger oils on hematological and biochemical variables of sea bass dicentrarchus labrax.this study was conducted to investigate the effects of garlic and ginger oils on hematological and biochemical health characteristics of sea bass dicentrarchus labrax. fish were exposed to garlic oil (0.01 or 0.02 ml/l), ginger oil (0.01 or 0.02 ml/l), or a combination of the two oils (each oil at a concentration of 0.005 or 0.01 ml/l) for 96 h via bath immersion. results showed that the red blood cell count, hematocrit (%), hemoglobin (hb) concentration (g/dl), mean corpuscular volume (μm(3)), ...201223043295
fish welfare assurance system: initial steps to set up an effective tool to safeguard and monitor farmed fish welfare at a company level.the objective was to take a first step in the development of a process-oriented quality assurance (qa) system for monitoring and safeguarding of fish welfare at a company level. a process-oriented approach is focused on preventing hazards and involves establishment of critical steps in a process that requires careful control. the seven principles of the hazard analysis critical control points (haccp) concept were used as a framework to establish the qa system. haccp is an internationally agreed ...201222278705
ozone disinfection of eggs from gilthead seabream sparus aurata, sea bass dicentrarchus labrax, red porgy, and common dentex dentex dentex.the risk of fish pathogen transmission via eggs can be reduced by disinfection in ozonated seawater. the aim of this study was to determine the suitable conditions for ozone disinfection of the eggs of gilthead seabream sparus aurata, sea bass dicentrarchus labrax, red porgy pagrus pagrus, and common dentex dentex dentex. the eggs were disinfected with a concentration (c) of 0.5 mg of ozone/l of water at four different exposure times (t = 2, 4, 8, and 16 min). the hatching rate was determined in ...201222838084
distribution of red-spotted grouper nervous necrosis virus (rgnnv) antigens in nervous and non-nervous organs of european seabass(dicentrarchus labrax) during the course of an experimental challenge.the distribution of red-spotted grouper nervous necrosis virus (rgnnv) antigens was examined by immunohistochemistry in the nervous and non-nervous organs of juvenile european seabass (dicentrarchus labrax) during the course of an intramuscular infection. histological changes resulting from the infection were evaluated from 3 days to 2 months post-infection. the specific antibody response was also studied 2 months post-challenge. viral proteins were present throughout the experimental period in ...201223271176
the in vitro effect of probiotic vagococcus fluvialis on the innate immune parameters of sparus aurata and dicentrarchus labrax.in this study we evaluated the effect of the probiotic vagococcus fluvialis on the cellular immune unspecific system of two different fish species of great interest in aquaculture such as gilthead sea bream (sparus aurata) and european sea bass (dicentrarchus labrax). leucocytes from head kidney of the two fish species were extracted and concentration adjusted to 10(7) cells ml(-1). phagocytic and respiratory burst activity and the peroxidase content of leucocytes were observed 30 min after incu ...201222864109
effects of dietary yeast saccaromyces cerevisiae on the antioxidant system in the liver of juvenile sea bass dicentrarchus labrax.the main goal of this work was to determine the effect of dietary live yeast saccharomyces cerevisiae on the oxidative status of sea bass dicentrarchus labrax juveniles. fishes were fed on three diets: the gm group were fed a diet containing lyophilized yeast grown on grape must, the cs group were fed a diet containing lyophilized yeast grown on cornstarch, and the control group were fed a diet without yeast. the activity of the main antioxidative enzymes, superoxide dismutase (sod), catalase (c ...201222484599
development of a homologous enzyme-linked immunosorbent assay for european sea bass fsh. reproductive cycle plasma levels in both sexes and in yearling precocious and non-precocious males.since the late 1980s, gonadotropins have been isolated and characterized in several fish species, but specific immunoassays for the follicle-stimulating hormone (fsh) have only been developed for a few. the present study reports the development and use of a specific and homologous competitive elisa for measuring fsh in european sea bass (dicentrarchus labrax) using a recombinant fsh and its specific antiserum. recombinant european sea bass fshβ and fsh heterodimer were produced in the methylotro ...201222227219
permanent genetic resources added to molecular ecology resources database 1 december 2011-31 january 2012.this article documents the addition of 473 microsatellite marker loci and 71 pairs of single-nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: barteria fistulosa, bombus morio, galaxias platei, hematodinium perezi, macrocentrus cingulum brischke (a.k.a. m. abdominalis fab., m. grandii goidanich or m. gifuensis ashmead), micropogonias furnieri, nerita melanotragus, nilaparvata lugens stål, sciaenops ocellat ...201222448966
cystatin b homolog from rock bream oplegnathus fasciatus: genomic characterization, transcriptional profiling and protease-inhibitory activity of recombinant protein.cysteine proteases are present in all living organisms and, in animals, function in a vast array of physiological and pathological processes. cysteine protease inhibitors act upon the cysteine proteases to regulate their activity. the cystatin superfamily of cysteine protease inhibitors has members represented in all living organisms studied to date. here, we report the identification of a new member of the family 1 cystatin in oplegnathus fasciatus rock bream (denoted as rbcyt b) and the charac ...201222626887
[cloning, physical and chemical property analysis of the japanese sea bass wap65-2 gene and its expression following vibrio harveyi infection].the warm temperature acclimation related 65 kda protein-2 (wap65-2), a teleost plasma glycoprotein, plays an important role in immune regulation against bacterial infection. here, for the first time we determined the full length cdna sequence of the japanese sea bass wap65-2 gene (1 601 bp in length excluding the 3'-polya tail). the sequence contains an open reading frame that encodes a protein of 436 amino acids with a molecular weight of 4.87×10(4). the predicted protein had a signal peptide i ...201223019029
a cd83-like molecule in sea bass (dicentrarchus labrax): molecular characterization and modulation by viral and bacterial infection.the cd83 cell surface marker is an important and intriguing component of immune system. it is considered the best marker for mature human dendritic cells, but it is also important for thymic development of t cells, and it also plays a role as a regulator of peripheral b-cell function and homeostasis. a cd83-like molecule was identified in sea bass (dicentrarchus labrax) by est sequencing of a thymus cdna library; the cd83 cdna is composed of 816 bp and the mature cd83 peptide consists of 195 ami ...201222554578
elemental composition of four farmed fish produced in portugal.farmed gilthead sea bream (sparus aurata), european sea bass (dicentrarchus labrax), rainbow trout (oncorhynchus mykiss) and turbot (psetta maxima) produced in portugal were analysed in order to characterize their elemental composition. atomic absorption (flame and cold vapour) and molecular absorption spectrometry techniques were used to determine all the studied elements. similar patterns of macro, trace and ultra trace elements were observed for all fish species. the main elements were potass ...201222574998
discriminating farmed gilthead sea bream sparus aurata and european sea bass dicentrarchus labrax from wild stocks through scales and otoliths.the study of mass and standard length (l(s) ) relationships showed that farmed individuals had higher values than wild fishes for both gilthead sea bream sparus aurata and european sea bass dicentrarchus labrax. such differences were more pronounced in larger individuals than smaller ones and were more noticeable in s. aurata than in d. labrax. additionally, differences in external characteristics of scales were detected between origins. a high proportion of farmed s. aurata had a regenerated nu ...201222551175
a microsatellite linkage map of striped bass (morone saxatilis) reveals conserved synteny with the three-spined stickleback (gasterosteus aculeatus).the striped bass (morone saxatilis) and its relatives (genus morone) are of great importance to fisheries and aquaculture in north america. as part of a collaborative effort to employ molecular genetics technologies in striped bass breeding programs, we previously developed nearly 500 microsatellite markers. the objectives of this study were to construct a microsatellite linkage map of striped bass and to examine conserved synteny between striped bass and three-spined stickleback (gasterosteus a ...201221968826
characterization of european sea bass transcripts by rna seq after oral vaccine against v. anguillarum.the european sea bass (dicentrarchus labrax) is, along with the gilthead sea bream (sparus aurata), one of the most extensively cultured species in european aquaculture productions. massive mortalities may be caused by bacterial or viral infections in intensive aquaculture production. evaluation of the efficacy of an oral vaccine against vibrio anguillarum (aquavac vibrio oral) in sea bass revealed specific immune gene expression profiles in the gut as well as protection of fish. in the present ...201222790793
effects on mortality and stress response in european sea bass, dicentrarchus labrax (l.), fed mannan oligosaccharides (mos) after vibrio anguillarum exposure.the effects of dietary mannan oligosaccharides (mos; 4 g kg(-1) ; bio-mos, alltech inc, usa) in diets for european sea bass, dicentrarchus labrax (l.), juveniles in relation to disease and stress resistance, combining intestinal infection with vibrio anguillarum and stress challenge by confinement, were assessed in this study. after 8 weeks of mos supplementation, fish were exposed to a pathogen challenge test against v. anguillarum by direct gut inoculation combined with a confinement stressor ...201222690841
treatment of vibriosis in european sea bass larvae, dicentrarchus labrax l., with oxolinic acid administered by bath or through medicated nauplii of artemia franciscana (kellogg): efficacy and residual kinetics.european sea bass larvae were challenged by bath with listonella anguillarum strain 332a, 2.5×10(7) cfuml(-1) for 1h. fish either received no treatment or oral treatment with artemia franciscana (kellog) nauplii enriched with oxolinic acid, or bath treatments with oxolinic acid. medication commenced 1day following challenge and was performed on days 1, 3 and 5 post-challenge at a dosage of 20mgl(-1) for 2h for bath treatments, while two doses each of 750 nauplii per fish were administered daily ...201222670590
bacterial host interaction of gfp-labelled vibrio anguillarum hi-610 with gnotobiotic sea bass, dicentrarchus labrax (l.), larvae.the location and cell damage caused by vibrio anguillarum, the causative agent of classical vibriosis, within the developing gut of the newly hatched sea bass, dicentrarchus labrax (l.), is unknown. a gnotobiotic sea bass model was used to investigate the early interactions of v. anguillarum with sea bass larvae. in the present study, germ-free sea bass larvae were orally exposed to a v. anguillarum hi-610 pathogen labelled with the green fluorescent protein (gfp-hi-610) and sampled at regular i ...201222324372
absence of viral hemorrhagic septicemia virus (vhsv) and infectious hemorrhagic necrosis virus (inhv) in a tunisian fish farm: a case study.beyond the obvious problems related to the sustainable management of wild stocks, current fish farming practices in the mediterranean area entail important environmental risks and potential outbreaks of fish diseases linked to massive translocations across regional boundaries. viral haemorrhagic septicaemia (vhs) and infectious hematopoietic necrosis (ihn) are well-known fish diseases caused by the vhsv and ihnv viruses, and positive cases are subject to obligatory reporting to the world organis ...201224834660
molecular cloning and characterization of sea bass (dicentrarchus labrax, l.) tapasin.mammalian tapasin (tpn) is a key member of the major histocompatibility complex (mhc) class i antigen presentation pathway, being part of the multi-protein complex called the peptide loading complex (plc). several studies describe its important roles in stabilizing empty mhc class i complexes, facilitating peptide loading and editing the repertoire of bound peptides, with impact on cd8(+) t cell immune responses. in this work, the gene and cdna of the sea bass (dicentrarchus labrax) glycoprotein ...201222119577
innovative vaccination protocol against vibriosis in dicentrarchus labrax (l.) juveniles: improvement of immune parameters and protection to challenge.the effect of vaccination on immune parameters of european sea bass, dicentrarchus labrax, is not fully established, as well as surveyed throughout rearing till the commercial size. furthermore, available information on the possible role of booster treatments is scarce. sea bass juveniles were vaccinated against listonella anguillarum using a commercial bivalent formulation administered by immersion (priming: 95 dph; booster: 165 dph) or by immersion (priming: 95 dph; booster: 165 dph) and subse ...201223277096
characterization of the transport of lysine-containing dipeptides by pept1 orthologs expressed in xenopus laevis oocytes.during digestion, dietary proteins cleaved in di and tri-peptides are translocated from the intestinal lumen into the enterocytes via pept1 (slc15a1) using an inwardly directed proton electrochemical gradient. the kinetic properties in various pept1 orthologs (dicentrarchus labrax, oryctolagus cuniculus, danio rerio) have been explored to determine the transport efficiency of different combinations of lysine, methionine, and glycine. species-specific differences were observed. lys-met resulted t ...201223268205
phylogenetic analysis of antimicrobial lactic acid bacteria from farmed seabass dicentrarchus labrax.the use of lactic acid bacteria (lab) in the prevention or reduction of fish diseases is receiving increasing attention. in the present study, 47 lab strains were isolated from farmed seabass ( dicentrarchus labrax ) and were phenotypically and phylogenetically analysed by 16s rdna and randomly amplified polymorphic dna - polymerase chain reaction (rapd-pcr). their antimicrobial effect was tested in vitro against a wide variety of pathogenic and spoilage bacteria. most of the strains isolated we ...201222439634
genomic characterization of the european sea bass dicentrarchus labrax reveals the presence of a novel uncoupling protein (ucp) gene family member in the teleost fish lineage.uncoupling proteins (ucp) are evolutionary conserved mitochondrial carriers that control energy metabolism and therefore play important roles in several physiological processes such as thermogenesis, regulation of reactive oxygen species (ros), growth control, lipid metabolism and regulation of insulin secretion. despite their importance in various physiological processes, their molecular function remains controversial. the evolution and phylogenetic distribution may assist to identify their gen ...201222577775
caspase-1 and il-1β processing in a teleost fish.interleukine-1β (il-1β) is the most studied pro-inflammatory cytokine, playing a central role in the generation of systemic and local responses to infection, injury, and immunological challenges. in mammals, il-1β is synthesized as an inactive 31 kda precursor that is cleaved by caspase-1 generating a 17.5 kda secreted active mature form. the caspase-1 cleavage site strictly conserved in all mammalian il-1β sequences is absent in il-1β sequences reported for non-mammalian vertebrates. recently, ...201223226286
evaluation of the probiotics bacillus subtilis and lactobacillus plantarum bioencapsulated in artemia nauplii against vibriosis in european sea bass larvae (dicentrarchus labrax, l.).two potential probiotics bacillus subtilis and lactobacillus plantarum were evaluated for use in aquaculture as preventive measures against vibriosis. in vitro evaluation of the probiotics using co-culture assays with the pathogen vibrio anguillarum and testing for the production of antibacterial substances showed the presence of antagonism and confirmed the production of antibacterial substances. both potential probiotics were administered to the live fish feed artemia franciscana nauplii, offe ...201222806117
toxic effects of harmful benthic dinoflagellate ostreopsis ovata on invertebrate and vertebrate marine organisms.harmful benthic microalgae blooms are an emerging phenomenon causing health and economic concern, especially in tourist areas. this is the case of the mediterranean sea, where ostreopsis ovata blooms occur in summer, with increasing regularity. ostreopsis species produce palytoxin (ptx) and analogues, and a number of deaths directly associated with the ingestion of ptx contaminated seafood have been reported. ptx is considered one of the most toxic molecules occurring in nature and can provoke s ...201222000703
first record of anisakid juveniles (nematoda) in the european seabass dicentrarchus labrax (family: moronidae), and their role as bio-indicators of heavy metal pollution.this study assessed the anisakid nematode distribution pattern in the fish collected from coasts of mediterranean sea, egypt, during the period september 2010-april 2011. two hundred thirty out of 300 (76.7%) dicentrarchus labrax (european seabass) marine fishes belonging to family moronidae were dissected and found to be infected with larva three nematodes. the larvae had been studied by light and scanning electron microscopy. the present work represents the first record of the presence of the ...201221845410
quantitative rt-pcr analysis and immunohistochemical localization of hsp70 in sea bass dicentrarchus labrax exposed to transport stress.in aquaculture, fish are exposed to stressful conditions, which cause an increased synthesis of heat shock proteins (hsps) at the cellular level. in this work we considered the expression of the constitutive and inducible forms of hsp70 as an indicator of stress caused by transport, during development of the sea bass (dicentrarchus labrax), a teleost fish of high value for aquaculture. qualitative rt-pcr analysis revealed expression of inducible hsp70 gene in larvae and fry (25, 40 and 80 days) ...201317664163
production and characterization of a continuous embryonic cell line from sea bass (dicentrarchus labrax l.).continuous cell lines represent an important tool both for biological studies and for their applications in marine biotechnology. in this article we describe the production and characterization of a continuous adherent cell line, named dlec, derived from early embryos of the european sea bass dicentrarchus labrax l. (actinopterygii, moronidae). cells were obtained by disrupting 2- to 12-hour-old embryos and culturing resulting cells at 18 degrees c in rpmi medium containing 5% fetal calf serum ( ...201316249966
tissue polyamine concentrations in the european sea bass (dicentrarchus labrax l.): change with age and season of the year.independently of the age of the european sea bass, putrescine and spermidine are much higher in liver and brain than in muscles, while spermine concentrations are more similar to one another. the polyamine concentrations are higher in 2 years old sea bass than in 1 year old fish except for heart spermidine and liver spermine. lowering in water temperature causes a decrease in the concentration of spermidine and spermine in all tissues examined. putrescine, however, increases in heart, caudal mus ...20132632463
[effects of hexavalent chromium at non-lethal concentrations on the enzymology of the intestine of salmo gairdneri and dicentrarchus labrax (pisces)].effects of non lethal concentrations of hexavalent chromium on intestinal enzymology of salmo gairdneri and dicentrarchus labrax (pisces). the effects of an exposure to potassium dichromate on intestinal enzyme activities (alkaline phosphatase, maltase, leucine amino peptidase and atpases) have been studied on a fresh water fish (salmo gairdneri) and a salt water fish (dicentrarchus labrax). fish were exposed at seasonal temperatures (13 or 21 degrees c) to toxic concentrations equal to 1/10 of ...20132977985
a complementary lc-esi-ms and maldi-tof approach for screening antibacterial proteomic signature of farmed european sea bass mucus.antibacterial protection in the mucus is provided by antimicrobial compounds and till now few numbers of amp and proteins were identified. herein, mass spectral profiling of fresh mucus from farmed sea bass (dicentrarchus labrax) using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometer (maldi-tof) and liquid chromatography mass spectrometry is investigated in order to survey the infective/healthy status of the mucus. we identify amp peptides of 2891.7, 2919.45 and 2286. ...201323643872
status and future perspectives of vaccines for industrialised fin-fish farming.fin fish farming is developing from extensive to intensive high industrial scale production. production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. vaccines can also have significant positive impact on the reduced usage of antibiotics. this was demonstrated when vaccines were introduced in norway for atlantic salmon (salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics con ...201323769873
does virulence assessment of vibrio anguillarum using sea bass (dicentrarchus labrax) larvae correspond with genotypic and phenotypic characterization?vibriosis is one of the most ubiquitous fish diseases caused by bacteria belonging to the genus vibrio such as vibrio (listonella) anguillarum. despite a lot of research efforts, the virulence factors and mechanism of v. anguillarum are still insufficiently known, in part because of the lack of standardized virulence assays.201323936439
luminal uptake of vibrio (listonella) anguillarum by shed enterocytes--a novel early defence strategy in larval fish.as adhesion and translocation through fish gut enterocytes of the pathogen vibrio (listonella) anguillarum are not well investigated, the effective cause of disease and mortality outbreaks in larval sea bass, dicentrarchus labrax, suffering from vibriosis is unknown. we detected v. anguillarum within the gut of experimentally infected gnotobiotic sea bass larvae using transmission electron microscopy and immunogold labelling. intact bacteria were observed in close contact with the apical brush b ...201323121135
heat shock protein 70 kda (hsp70) increase in sea bass (dicentrarchus labrax, l 1758) thymus after vaccination against listonella anguillarum.heat shock proteins 70 kda (hsp70) and apoptosis were investigated in thymus of sea bass juveniles (dicentrarchus labrax) subsequently to a vaccination against listonella (syn. vibrio) anguillarum. hsp70 expression was measured by immunohistochemistry and immunoenzymatic methods, resulting in increase in hsp70 after bath immunization and persistent in fish exposed to an intraperitoneal (i.p.) booster. the hsp70 increase in thymus was suggested as induction in lymphocytic cells, to be related to ...201323053607
gill morphology in two mediterranean sea fishes of similar feeding preferences: sea bream (sparus aurata l) and sea bass (dicentrarchus labrax).the aim of the present study was to describe the gill morphology of the gilthead sea bream and sea bass fishes that showed the same feeding habits. our study focused on the gross morphological, scanning and light microscopical features of the gills, where a many differences in the gill arches and gill rakers surface in both species were explained. our results marked that; there was an interbranchial median elevation appeared in sea bream. the gill arches length and the gaps between decreased med ...201323475751
isolation of a novel gene from photobacterium damselae subsp. piscicida and analysis of the recombinant antigen as promising vaccine candidate.photobacterium damselae subsp. piscicida (pdp) is the causative agent of fish pasteurellosis, a bacterial disease causing important losses in marine aquaculture. vaccines against the pathogen can be a way to control the infection and avoid antibiotic treatments. however, a satisfactory protective vaccine against fish pasteurellosis is not commercially available. in this study, a biotechnogical approach based on reverse vaccinology has been used to identify potential vaccine candidates for the de ...201323219776
phenotypic characterization and rapd fingerprinting of vibrio parahaemolyticus and vibrio alginolyticus isolated during tunisian fish farm outbreaks.the genus vibrio is characterized by a large number of species and some of them are human pathogens causing gastrointestinal and wound infections through the ingestion or manipulation of contaminated fishes and shellfish including vibrio parahaemolyticus and vibrio alginolyticus. in this study, we reported the phenotypic and molecular characterization of 9 v. parahaemolyticus and 27 v. alginolyticus strains isolated from outbreaks affecting cultured gilthead sea bream (sparus aurata l.) and sea ...201322684973
molecular epidemiology of betanodaviruses isolated from sea bass and sea bream cultured along the tunisian coasts.viral nervous necrosis (vnn) is a serious viral disease affecting farmed sea bass (dicentrarchus labrax). only scarce molecular data are available on the disease-causing betanodavirus populations in tunisia. therefore, we carried out the first molecular survey of betanodaviruses in farmed sea bass and sea bream (sparus aurata) along the tunisian coasts. among 81 samples from five farms, 20 tested positive with rt-pcr, not only in clinical cases but also in asymptomatic fish before and during out ...201323288625
cytokine expression in head-kidney leucocytes of european sea bass (dicentrarchus labrax l.) after incubation with the probiotic vagococcus fluvialis l-21.the european sea bass (dicentrarchus labrax l.) is one of the most extensively farmed marine fish in the mediterranean sea. under the high-density condition, common in aquaculture, the infectious diseases can cause significant economic losses. probiotics are presented as an alternative to antibiotics for the control of aquaculture diseases. this study used real-time pcr to investigate in vitro the dynamic of expression of immune-related genes in sea bass after incubation with live and inactivate ...201323927874
apoptosis in thymus of teleost fish.the presence and distribution of apoptotic cells during thymus development and in adult were studied by in situ end-labelling of fragmented dna in three temperate species carp (cyprinus carpio), sea bass (dicentrarchus labrax) and dusky grouper (epinephelus marginatus) and in the adult thymus of three antarctic species belonging to the genus trematomus spp. during thymus development some few isolated apoptotic cell (ac) firstly appeared in the central-external part of the organ (carp: 5 days ph; ...201323639932
somatotropic axis genes are expressed before pituitary onset during zebrafish and sea bass development.the somatotropic axis, or growth hormone-insulin-like growth factor-1 (gh-igf-1) axis, of fish is involved in numerous physiological process including regulation of ionic and osmotic balance, lipid, carbohydrate and protein metabolism, growth, reproduction, immune function and behavior. it is thought that gh plays a role in fish development but conflicting results have been obtained concerning the ontogeny of the somatotropic axis. here we investigated the developmental expression of gh, gh-rece ...201324055560
expression of kisspeptins in the brain and pituitary of the european sea bass (dicentrarchus labrax).kisspeptins are now considered key players in the neuroendocrine control of puberty and reproduction, at least in mammals. most teleosts have two kiss genes, kiss1 and kiss2, but their sites of expression are still poorly documented. as a first step in investigating the role of kisspeptins in the european sea bass, a perciform fish, we studied the distribution of kiss1 and kiss2-expressing cells in the brain of males and females undergoing their first sexual maturation. animals were examined at ...201322886357
cloning and expression analysis of follicle-stimulating hormone and luteinizing hormone receptor during the reproductive cycle in korean rockfish (sebastes schlegeli).full-length cdna sequences encoding the receptors for follicle-stimulating hormone (fshr) and luteinizing hormone (lhr) were isolated from ovary of korean rockfish (sebastes schlegeli) using reverse transcription-polymerase chain reaction (pcr) and rapid amplification of cdna ends procedures. the cdna of the krfshr encodes a predicted protein of 703 amino acids that showed the greatest homology with european seabass (dicentrarchus labrax) (78 %) and gilthead seabream (sparus aurata) (73 %). the ...201322843313
disposition of [u-2h7]glucose into hepatic glycogen in rat and in seabass.the stimulation of hepatic glycogenesis is a ubiquitous response to a glucose challenge and quantifying its contribution to glucose uptake informs its role in restoring euglycemia. glycogenesis can be quantified with labeled water provided that exchange of glucose-6-phosphate hydrogen 2 (g6p-h2) and body water via glucose-6-phosphate isomerase, and exchange of positions 4, 5 and 6 hydrogens (g6p-h456) via transaldolase, are known. these exchanges were quantified in 24-h fasted rats (rattus norve ...201323838145
first insight into exploration and cognition in wild caught and domesticated sea bass (dicentrarchus labrax) in a maze.european sea bass aquaculture is so recent that very little is known on the effects of the early steps of its domestication. behavioural parameters are sensitive indicators of the domestication process since they are generally impacted as soon as the first generation. the present work compared wild-caught and domesticated sea bass juvenile swimming activity, exploration and ability to learn to discriminate between two 2-d objects associated to a simple spatial task that enabled the tested indivi ...201323805190
influence of temperature in thermal and oxidative stress responses in estuarine fish.the influence of increasing temperatures in thermal and oxidative stress responses were studied in the muscle of several estuarine fish species (diplodus vulgaris, diplodus sargus, dicentrarchus labrax, gobius niger and liza ramada). selected fish were collected in july at the tagus estuary (24±0.9°c; salinity of 30±4‰; ph=8). fish were subjected to a temperature increase of 1°c.h(-1) until they reached their critical thermal maximum (ctmax), starting at 24°c (control temperature). muscle sample ...201323774589
enteroendocrine profile of α-transducin immunoreactive cells in the gastrointestinal tract of the european sea bass (dicentrarchus labrax).in vertebrates, chemosensitivity of nutrients occurs through the activation of taste receptors coupled with g-protein subunits, including α-transducin (g(αtran)) and α-gustducin (g(αgust)). this study was aimed at characterising the cells expressing g(αtran) immunoreactivity throughout the mucosa of the sea bass gastrointestinal tract. g(αtran) immunoreactive cells were mainly found in the stomach, and a lower number of immunopositive cells were detected in the intestine. some g(αtran) immunorea ...201323748963
effects of dopaminergic system activation on feeding behavior and growth performance of the sea bass (dicentrarchus labrax): a self-feeding approach.dopamine is synthesized from l-dopa and subsequently processed into norepinephrine and epinephrine. any excess neurotransmitter can be taken up again by the neurons to be broken down enzymatically into dopac. the effect of dopamine on mammalian food intake is controversial. mice unable to synthesize central dopamine die of starvation. however, studies have also shown that central injection of dopamine inhibits food intake. the effect of dopaminergic system in the fish feeding behavior has been s ...201323747830
the clc-3 chloride channel and osmoregulation in the european sea bass, dicentrarchus labrax.dicentrarchus labrax migrates between sea (sw), brackish and fresh water (fw) where chloride concentrations and requirements for chloride handling change: in fw, fish absorb chloride and restrict renal losses; in sw, they excrete chloride. in this study, the expression and localization of clc-3 and na(+)/k(+)-atpase (nka) were studied in fish adapted to sw, or exposed to fw from 10 min to 30 days. in gills, nka-α1 subunit expression transiently increased from 10 min and reached a stabilized inte ...201323292336
metabolism of the polycyclic musk galaxolide and its interference with endogenous and xenobiotic metabolizing enzymes in the european sea bass (dicentrarchus labrax).this study investigates the metabolism and mode of action of galaxolide (hhcb) in the european sea bass -dicentrarchus labrax- following a single intraperitoneal injection of 50 mg hhcb/kg body weight. in addition, a group of fish was injected with 50 mg/kg of ketoconazole (kcz), a fungicide that is known to interfere with different cyp isoenzymes. hhcb was actively metabolised by sea bass and acted as a weak inhibitor of the synthesis of oxyandrogens in gonads of male fish. both, hhcb and a hyd ...201323274450
multiple vitellogenin yolk precursors in european sea bass (dicentrarchus labrax). 201325141754
microparticles delivery system in gnotobiotic european sea bass larvae (dicentrarchus labrax, l. 1758). 201325141751
cholecystokinin and tryptic enzyme activity in sea bass (dicentrarchus labrax) larvae: a regulatory loop and the impact of feeding regimes. 201325141739
use of phaeobacter sp. probiotic bacteria for the rearing of sea bass larvae (dicentrarchus labrax). 201325141684
correlation of saddleback syndrome with deformities of the pelvic fins and lateral line in european sea bass, dicentrarchus labrax. 201325141647
oxidative stress in sea bass dicentrarchus labrax larvae: interaction of high dietary dha contents and several antioxidant nutrients. 201325141617
consumer preferences regarding the introduction of new organic products. the case of the mediterranean sea bass (dicentrarchus labrax) in italy.the introduction of new products on the market poses several challenges; in particular, whether the characteristics of the proposed product will be judged positively by potential consumers. this paper analyses the preferences of consumers regarding the introduction on the italian market of a new product: organic mediterranean sea bass. the aim of this study is to assess the importance given by consumers to four main characteristics of sea bass (country of origin, size, production method - organi ...201323268110
sarcoplasmic reticulum: a key factor in cardiac contractility of sea bass dicentrarchus labrax and common sole solea solea during thermal acclimations.this study investigated the effects of acclimation temperature upon (i) contractility of ventricular strips (ii) calcium movements in ventricular cardiomyocytes during excitation-contraction coupling (ecc), and (iii) the role of the sarcoplasmic reticulum (sr) in myocardial responses, in two marine teleosts, the sea bass (dicentrarchus labrax) and the common sole (solea solea). because of the different sensitivities of their metabolism to temperature variation, both species were exposed to diffe ...201323263664
luteinizing hormone plasmid therapy results in long-lasting high circulating lh and increased sperm production in european sea bass (dicentrarchus labrax).the present work aimed at evaluating the potential of intramuscular injection of a hormone-coding gene as an approach for gene therapy in fish. a plasmid containing luteinizing hormone (lh) in a single-chain (sc) form, pcmv-sclh, was chosen as the coding gene, and sea bass was chosen as the target species. in vivo injection of pcmv-sclh in muscle of juvenile sea bass rendered plasma lh levels higher than 50 ng/ml in 40% of the injected fish, while these lh levels were only detected in 4% of cont ...201323255338
fatty acid metabolism in european sea bass (dicentrarchus labrax): effects of n-6 pufa and mufa in fish oil replaced diets.monounsaturated fatty acids (mufa)-rich and n-6 polyunsaturated fatty acid (n-6 pufa)-rich vegetable oils are increasingly used as fish oil replacers for aquafeed formulation. the present study investigated the fatty acid metabolism in juvenile european sea bass (dicentrarchus labrax, 38.4 g) fed diets containing fish oil (fo, as the control treatment) or two different vegetable oils (the mufa-rich canola/rapeseed oil, co; and the n-6 pufa-rich cottonseed oil, cso) tested individually or as a 50 ...201323212581
physiological pathways involved in nutritional muscle dystrophy and healing in european sea bass (dicentrarchus labrax) larvae.the potential muscle regeneration after nutritional dystrophy caused by high dietary dha contents in fish and the physiological pathways involved are still unknown. to better understand this process, an experiment was conducted for 3 weeks in 14 day-old european sea bass larvae using different dha ratios (1 or 5%). after this period, part of the larvae fed 5% dha diet was switched to 1% dha diet ("wash-out") for another 2 weeks. larvae fed 5% dha diet showed altered oxidative status as indicated ...201323202658
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