Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| common bacterial pneumonitis in infants. determining the etiology and tailoring the treatment. | in infantile pneumonia, we recommend close attention to the history and physical examination. baseline studies, including cbc, esr, blood cultures, and chest film, should be performed at onset and repeated as warranted. nasopharyngeal secretions or washings should be drawn by means of gentle suction and specimens sent for gram stain, fluorescent antibody stain for respiratory syncytial virus, and culture for bacteria and for viruses if possible. acute and convalescent serum specimens should be o ... | 1986 | 3511465 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by a biotin-avidin elisa more sensitive than the fluorescent antibody technique. | the presence of respiratory syncytial virus (rsv) was investigated by immunofluorescent antibody (if) technique and by a biotin/avidin (ba) elisa in 156 samples of nasopharyngeal secretions (nps) obtained from infants and small children with acute respiratory disease. of 70 rsv-if-positive nps, 68 were positive by ba-elisa. of 86 rsv-if-negative nps, eight were positive by ba-elisa. ba-elisa could detect 0.5-1.0 ng rsv-protein. | 1986 | 3512768 |
| resistance to human respiratory syncytial virus (rsv) infection induced by immunization of cotton rats with a recombinant vaccinia virus expressing the rsv g glycoprotein. | a cdna copy of the g glycoprotein gene of human respiratory syncytial virus (rsv) was placed under control of a vaccinia virus promoter and inserted into the thymidine kinase locus of the vaccinia virus genome. the recombinant vaccinia virus retained infectivity and expressed a 93-kda protein that migrated with the authentic rsv g glycoprotein upon polyacrylamide gel electrophoresis. glycosylation of the expressed protein and transport to the cell surface were demonstrated in the absence of othe ... | 1986 | 3513191 |
| rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay. | a commercial enzyme immunoassay (eia) for the rapid detection of respiratory syncytial virus (rsv) in respiratory secretions was evaluated by comparison with both virus isolation in hep-2 cells and indirect immunofluorescence (ifa) staining of exfoliated respiratory cells. initial examination of 80 nasopharyngeal aspirates collected from infants with acute respiratory illness showed that the rsv eia was positive for 21 of 24 specimens positive by virus isolation or ifa (87.5% sensitivity) and ne ... | 1986 | 3514658 |
| evaluation of clinical specimens for the presence of respiratory syncytial virus antigens using an enzyme immunoassay. | an enzyme-linked immunoassay (eia) was developed for the detection of respiratory syncytial virus (rsv) antigen in nasopharyngeal secretions. this assay, which employs goat and rabbit anti-rsv as the capture and detector antibodies respectively, was used in a retrospective evaluation of frozen clinical specimens from children. the eia results were compared with those of virus isolation in cell culture and direct fluorescent antibody staining performed at the time of specimen collection. the sens ... | 1986 | 3517226 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay, indirect immunofluorescence, and virus isolation: a comparative study. | an enzyme-linked immunosorbent assay (elisa) was developed for the detection of respiratory syncytial virus (rsv) antigens in nasopharyngeal secretions (nps) from children with acute respiratory disease. antisera against rsv nucleocapsids were used as immunoreagents for this test system. the results obtained by rsv antigen elisa were compared to those of indirect immunofluorescence (if) and tissue culture virus isolation (tc). of the 404 nps obtained, 278 were tested in parallel by elisa and if ... | 1986 | 3522805 |
| use of egg-yolk antibody for detection of respiratory syncytial virus in nasal secretions by elisa. | egg-yolk immunoglobulins extracted from the eggs of hens immunized with respiratory syncytial virus (rsv) have been used as a reagent in double sandwich elisa for detecting rsv in nasal secretions. the sensitivity of virus detection was the same in indirect elisa, using rabbit anti chicken globulin conjugate, as when biotinylated yolk globulin and labeled avidin were used for detection. the specificity of elisa for detecting rsv using yolk antibody was similar to that achieved by indirect immuno ... | 1986 | 3524512 |
| value of rapid diagnosis of respiratory syncytial virus infection on management of small infants. | respiratory syncytial virus (rsv) is a common cause of infection in infancy and early childhood. a presumptive diagnosis of rsv infection can frequently be made on clinical grounds. confirmation can be made by viral culture, which may take 3 to 7 days. immunofluorescent assay (ifa) is a specific and sensitive test that can provide laboratory confirmation of rsv infection the same day. rapid diagnosis of rsv infection may have implications regarding prevention of nosocomial spread of rsv, early i ... | 1986 | 3524958 |
| normal and malignant cells, including neurons, deposit plasminogen activator on the growth substrata. | the results of four different assay methods showed that both normal and malignant plasminogen activator-secreting cells deposited substantial amounts of this protease on tissue-culture substrata, including collagen coatings. the cells studied were rous sarcoma virus (rsv)-transformed vole fibroblasts, a malignant neural cell line (ng108-15) capable of neurite formation, and normal mouse-regenerating sensory neurons. deposited plasminogen activator was detected by a fibrin overlay assay at sites ... | 1986 | 3527728 |
| expression of the f glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the f and g glycoproteins to host immunity. | a cdna clone representing the mrna coding sequence of the fusion glycoprotein (f) gene of human respiratory syncytial virus (rsv) was constructed and inserted into the thymidine kinase gene of vaccinia virus (wr strain) under the control of a vaccinia virus promoter. the resulting recombinant vaccinia virus, vaccinia f, expressed the f1 and f2 cleavage products (48 and 20 kda, respectively) of the f glycoprotein in cell culture. f1 and f2 were indistinguishable from their authentic rsv counterpa ... | 1986 | 3532115 |
| strategy for efficient detection of respiratory viruses in pediatric clinical specimens. | direct immunofluorescence (if) with a polyclonal respiratory syncytial virus (rsv)-specific antibody preparation was used for antigen detection during the 1982-1983 rsv season (155 specimens) and gave an overall sensitivity of 94% with 87% specificity compared with viral culture. indirect if was used in the 1983-1984 season (265 specimens) and exhibited sensitivity of 96% with specificity of 79%. during these two seasons, 42 of 224 (18.8%) specimens that were if-negative for rsv grew viruses oth ... | 1986 | 3536275 |
| comparison of two new tests for rapid diagnosis of respiratory syncytial virus infections by enzyme-linked immunosorbent assay and immunofluorescence techniques. | the sensitivity and the specificity of two new commercial reagent tests, an indirect fluorescent antibody test (fat) with a mouse monoclonal antibody (mab) against respiratory syncytial virus (rsv) and an enzyme-linked immunosorbent assay (elisa) rsv antigen detection kit, were determined by a comparison of results from these tests with those of tissue culture isolation and an indirect fat with bovine polyclonal antibody (bpa). of 251 nasal aspirates from infants with suspected rsv infection, po ... | 1986 | 3536993 |
| rapid diagnosis of respiratory syncytial virus infection by antigen immunofluorescence detection with monoclonal antibodies and immunoglobulin m immunofluorescence test. | during a respiratory syncytial virus (rsv) infection outbreak in a pediatric hospital, diagnosis was made by immunofluorescence on smears by using an anti-rsv monoclonal antibody (ifm). immunoglobulins m and g were titrated by indirect immunofluorescence on hep-2 cells infected with an rsv strain. the ifm was sensitive (89%) and specific (75%) when compared with the cell culture method. we showed that the specimens which were found positive by ifm and negative by cell culture were truly positive ... | 1986 | 3536995 |
| concomitant immunity leads to retardation or acceleration of the growth of rous sarcomas in genetically resistant or susceptible inbred lines of chickens. | we report results on the age-dependent effect of the b (mhc) genotype on resistance to the growth of a second rsv tumour in chickens from highly inbred lines genetically resistant or susceptible to progressive growth of primary rsv-induced tumours. furthermore, the second inoculation of rsv caused either accelerated or retarded growth of the primary tumours induced by the same virus. these two types of response to a second challenge with rsv were controlled by alleles of the chicken major histoc ... | 1986 | 2852125 |
| monoclonal antibodies to the v-fes product and to feline leukemia: virus p27 interspecies-specific determinants encoded by feline sarcoma viruses. | monoclonal antibodies to p27 gag and v-fes specific determinants on the gag-onc poly-protein encoded by snyder-theilen feline sarcoma virus (st-fesv) were prepared. in order to obtain hybridoma clones specific to the antigenic determinants encoded by the fesv genome, lou rats were immunized with st-fesv-transformed, virus-nonproducing syngeneic cells, and boosted with either the same cells or affinity-purified feline leukemia virus (felv) p27. three distinct clones reactive to both felv p27 and ... | 1986 | 3024406 |
| comparison of the relative importance of tyrosine-specific vinculin phosphorylation and the loss of surface-associated fibronectin in the morphology of cells transformed by rous sarcoma virus. | we have investigated the relative importance of tyrosine-specific phosphorylation of vinculin and the loss of surface-associated fibronectin in the maintenance of the rounded morphology characteristic of chick embryo fibroblasts (cef) transformed by rous sarcoma virus (rsv). to address this question we have examined the interaction of cef and rsv-cef in vitro with exogenously added fibronectin in both 3-day culture experiments and short-term, 3-h spreading experiments. we report that the additio ... | 1986 | 3025229 |
| expression strategies of the yeast retrotransposon ty: a short sequence directs ribosomal frameshifting. | the ty element of yeast is a member of a class of eukaryotic transposons which bear a striking resemblance to retroviral proviruses in their structure and expression strategies (1,2). a direct comparison can be drawn between the production of a fusion protein encoded by ty, resulting from a frameshift event which fuses two out-of-phase open reading frames tya and tyb, and the production of pr180gag-pol in a retrovirus such as rous sarcoma virus (rsv) (3,4). we present data which shows, definitiv ... | 1986 | 3020502 |
| [structural polymorphism of the provirus integrated in the genome of mammalian cells transformed by rous sarcoma virus]. | the structural organization of integrated rous sarcoma virus (rsv) genome in 8 different mammalian tumour cell lines has been studied. the different types of provirus rearrangements were found, e.g.: breakpoint mutations, deletions of rsv structural genes, elimination of the entire viral genome. the structural changes of proviruses appeared during long-term cultivation of tumour cell lines in vitro. the ability of transformed cells to produce complete infectious rsv correlate with the structural ... | 1986 | 3022121 |
| [transfer of active oncogenes and promoters into the mouse cell genome]. | different cell dna's (normal nih 3t3 dna; human osteosarcoma cell dna; human malignant glioma cell dna with amplified c-ha-ras) were cotransfected onto nih 3t3 cells with cloned long terminal repeat (ltr) sequences of rous sarcoma virus. ltr rsv and normal nih 3t3 dna c-fos oncogen expression was detected in tumors induced in nude mice. in the same system human tumour cell dna with amplified c-ha-ras gene was used, that to the integration and amplification of ltp sequences with simultaneous main ... | 1986 | 3022122 |
| clinical observations on mechanical ventilation for respiratory failure in bronchiolitis. | an unusually large number of infants (82) were admitted to stanford university hospital from november 1, 1983, through may 31, 1985, with a diagnosis of bronchiolitis requiring oxygen therapy. a larger percentage of these infants (17/82 = 21%) than generally expected required mechanical ventilation for respiratory failure. fourteen infants had respiratory syncytial virus (rsv) infections, and three had parainfluenza virus infections. ten patients had respiratory difficulties as neonates. the mec ... | 1986 | 3022224 |
| synthesis in vitro of a seven amino acid peptide encoded in the leader rna of rous sarcoma virus. | sequences of avian retroviral rnas suggest that short open reading frames in the putatively untranslated leader sequences might direct the synthesis of small peptides. previous analyses indicate that translation of rous sarcoma virus (rsv) rna in vitro faithfully reflects translation of the viral rna in the chick cell. accordingly, we sought to determine if the heptapeptide lp1, encoded in the open reading frame closest to the 5' end of rsv rna, could be synthesized in vitro since this would str ... | 1986 | 3023636 |
| genetic lesions involved in temperature sensitivity of the src gene products of four rous sarcoma virus mutants. | the src genes of four rous sarcoma virus (rsv) mutants temperature-sensitive (ts) for cell transformation were analyzed. the mutant src genes were cloned into a replication-competent rsv expression vector, and the contribution of individual mutations to the ts phenotype was assessed by in vitro recombination with wild-type src sequences. three of the mutants, which were derived from the schmidt-ruppin strain of rsv, each encoded two mutations within the conserved kinase domain. in all three case ... | 1986 | 3023675 |
| aberrant mhc antigens in a sarcoma virus-induced mouse tumour. | from a series of mouse sarcomata, newly induced by rous sarcoma virus (rsv), the doh cell line was shown to lack expression of syngeneic h-2kd and dk antigens (noll et al., 1986). it unexpectedly displayed determinants specific for h-2kk molecules. interferon treatment of doh stimulated the expression of h-2kk determinants and also the display of some, but not all, determinants of the syngeneic h-2kd molecules. h-2dk expression was not stimulated. southern blot hybridization of genomic dna diges ... | 1986 | 3029231 |
| rs virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay. | two techniques for rapid diagnosis, immunofluorescence (ifat) and enzyme immunoassay (eia), have been compared with virus isolation in tissue culture for the detection of respiratory syncytial virus (rsv) in specimens of nasopharyngeal secretions. the specimens were obtained from children under five years of age suffering from acute respiratory illness, during a period of six months from january to june 1982. of 471 specimens examined 54 (11.5%) were positive by virus isolation and 180 (38.2%) w ... | 1986 | 3295470 |
| tissue-specific expression in transgenic mice of a fused gene containing rsv terminal sequences. | transgenic mice were generated with prsv-cat, a chimeric gene construct containing the long terminal repeat of rous sarcoma virus (rsv) linked to the bacterial gene encoding chloramphenicol acetyltransferase (cat). cat expression, detected in adult animals of five independent strains, was preferentially directed to organs rich in tendon, bone, and muscle. this pattern reflects the disease specificity of the intact virus and suggests that the tissue tropism of rsv is determined at least in part b ... | 1986 | 3006249 |
| rous sarcoma virus transforming protein lacking myristic acid phosphorylates known polypeptide substrates without inducing transformation. | mutagenesis of glycine 2 of p60src, the transforming protein of rous sarcoma virus (rsv), yields a protein that is neither myristylated nor bound to cellular membranes. although these mutant viruses retain full tyrosine protein kinase activity, they are transformation-defective. we examined in detail tyrosine phosphorylation of cellular polypeptides and the phenotype induced by infection with two such viruses. infection failed to cause growth in agar, cytoskeletal reorganization, or changes in f ... | 1986 | 3006923 |
| intracellular k+ and the expression of transformation parameters by chick cells transformed with the bryan strain of rous sarcoma virus. | as normal chick embryo (ce) cells entered quiescence the intracellular concentrations of both na+ and k+ declined. comparable decreases in intracellular concentrations of na+ and k+ were not observed in ce cells transformed by either the schmidt-ruppin (sr) or the bryan (b) strain of rous sarcoma virus (rsv). intracellular concentrations of na+ were higher in sr-rsv-transformed ce cells than in b-rsv-transformed cells and uninfected ce cells at all times after plating. in contrast, intracellular ... | 1986 | 3008417 |
| ecto-protein kinase activities in normal and transformed cells. | the incubation of intact uninfected and rous sarcoma virus (rsv)-transformed chicken cells (sr-rsv-a) with micromolar amounts of [gamma-32p]atp under physiological conditions resulted in the radioactive phosphorylation of a variety of proteins. according to the experimental protocol the detectable phosphorylation was restricted to atp utilization at the cell surface and was catalyzed by surface located protein kinase (pk). serine- and to a lesser extent, threonine residues were phosphorylated. w ... | 1986 | 3009192 |
| genetic structure of the endogenous proviruses and expression of the gag gene in brown leghorn chickens. | seven loci of endogenous proviruses were detected in the genome of brown leghorn chickens. sets of endogenous proviruses in dna of the chicken embryos examined were identified by blot hybridization with 32p-labelled dna of rsv and ecori restriction endonuclease digestion. comparison of the results showed that only one locus (a) of endogeneous provirus was associated with a gs+ phenotype as determined by the immunoperoxidase reaction and antibodies against gag gene products of rsv. restriction en ... | 1986 | 3009239 |
| tests of association of immunoglobulin allotype genes and viral oncogenesis in chickens. | chickens from regional poultry research laboratory (rprl) inbred line 6(3) are resistant to virally-induced marek's disease (md) and lymphoid leukosis (ll) and are relatively strong regressors of virally-induced rous sarcomas. in contrast, rprl line 100 chickens are highly susceptible to md and ll and are weaker regressors of rous sarcomas than line 6(3). rprl lines 100 and 6(3) differ for alleles at the igg-1 (g-1) allotype locus, but have identical igm-1 (m-1) allotype alleles. to test the pos ... | 1986 | 3009316 |
| transcriptional activation encoded by the v-fos gene. | we present evidence that the fos oncogene encodes a transcriptional trans-activation function. trans-activation was assayed by cotransfection into nih 3t3 mouse fibroblasts of v-fos dna containing plasmids together with a plasmid containing a test promoter. three v-fos dnas were used: (i) pfbr-1, a plasmid containing the fbr proviral sequences; (ii) pfbj-2, a plasmid harboring the fbj proviral sequences; (iii) pmf-j, a plasmid containing the fbj fos sequences linked to a mouse metallothionein pr ... | 1986 | 3010284 |
| an anticatalytic monoclonal antibody to avian plasminogen activator: its effect on behavior of rsv-transformed chick fibroblasts. | a monoclonal antibody has been raised against the serine protease, plasminogen activator (pa) produced by rous sarcoma virus-transformed chick embryo fibroblasts (rsvcef), and selected for its ability to inhibit the catalytic activity of pa. the high specificity and anticatalytic nature of the antibody has allowed probing of the direct role of pa in cellular behavior. microgram quantities of monoclonal igg inhibit the overgrowth and the morphological changes associated with rsvcef transformation ... | 1986 | 3011282 |
| activation of the pp60c-src kinase during differentiation of monomyelocytic cells in vitro. | the proto-oncogene c-src, the cellular homolog of the rous sarcoma virus (rsv) transforming gene v-src, is expressed in a tissue-specific and age-dependent manner. its physiological function, although still unknown, appears to be more closely related to differentiation processes than to proliferation processes. to obtain more information about the physiological role of the c-src gene in cells, we have studied differentiation-dependent alterations using the human hl-60 leukaemia cell line as a mo ... | 1986 | 3011421 |
| expression of integrated rous sarcoma viruses: dna rearrangements 5' to the provirus are common in transformed rat cells but not seen in infected but untransformed cells. | the study of rous sarcoma virus (rsv)-infected rat cell clones offers a novel approach to unravelling the mechanisms controlling eukaryotic gene expression. rsv-transformed rat cell clones frequently contain duplicated proviral sequences immediately upstream of an intact provirus. this category of proviral rearrangement is not seen in cells that remain untransformed after rsv infection nor in subsequently segregating transformants. these results suggest that such rearrangements occur during or s ... | 1986 | 3011422 |
| the use of rous sarcoma virus transformation mutants with differing tyrosine kinase activities to study the relationships between vinculin phosphorylation, pp60v-src location and adhesion plaque integrity. | tyrosine-specific phosphorylation of cellular proteins has been implicated in the neoplastic transformation of cells by rous sarcoma virus (rsv). one of the putative substrates for the src gene product (pp60v-src) of rsv is the cytoskeletal protein vinculin, giving rise to the hypothesis that tyrosine-specific phosphorylation of vinculin disrupts adhesion plaque integrity, leading to the characteristic rounded morphology of rsv-transformed cells. we have investigated this hypothesis by analysing ... | 1986 | 3011478 |
| [viral respiratory infections in children: new diagnostic methods for early detection. initial results of a pilot project in switzerland]. | the new methods of rapid viral diagnosis make it possible to specify a number of the most prevalent agents of respiratory tract infections within 24 hours. the techniques are based on the immunological detection of antigens of respiratory syncytial (rsv), adeno, parainfluenza type 1, 2 and 3, as well as of influenza a and b viruses in nasopharyngeal secretions. during a one-year period we have used these methods to evaluate diagnostically 1541 outpatients presenting with upper and lower respirat ... | 1986 | 3012772 |
| fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. | the complete sequences of the fusion (f) mrna and protein of human parainfluenza virus type 3 (pf3) were determined from overlapping cdna clones. to confirm the cdna sequence, the complete sequence of the f gene was determined independently by dideoxynucleotide sequencing of genomic rna using synthetic oligonucleotide primers. the mrna contains 1845 nucleotides, exclusive of poly (a), has an unusually long (193-nucleotide) 5' nontranslated region, and encodes an f0 protein of 539 amino acids. th ... | 1986 | 3012869 |
| [virus-specific proteins in cells transformed by the chicken sarcoma virus d6]. | the structural proteins and the oncogene product of the avian sarcoma virus (asv) d6 were analyzed by the radioimmunoprecipitation. adv d6 was obtained from the chemically induced tumour. asv d6 contains all the structural proteins found in rsv, but some of them (p19, gp 85) differ from those of rsv. the product of asv d6 oncogene is pp65src. the protein kinase activity of this src protein is identical to that of wt pp60src. the nature of some other proteins, which can be phosphorylated in this ... | 1986 | 3013582 |
| virus-specific nucleotide sequences in duck cells transformed by chicken and duck-adapted rous sarcoma virus. | adaptation of pr-rsv-c on duck cells results in successful and efficient replication of the adapted virus in duck cells. the adapted variant, dapr-rsv-c, was compared with the parental chicken-cell derived pr-rsv-c. no differences in the efficiency of integration and in the number of integrated proviral copies in duck cells were found. however, the structure of proviral dna of the adapted virus was different. whereas ecori and hindiii digestion showed no differences between chicken-cell derived ... | 1986 | 3013702 |
| characterization of monoclonal antibodies to avian leukosis viruses. | hybridoma cell lines secreting monoclonal antibody (mca) to avian leukosis virus (alv) structural proteins p27 and p19 have been established. in an indirect enzyme-linked immunosorbent assay (elisa), mca 6al20 (igg1 isotype) reacted with rpl-40 (alv subgroup a), avian myeloblastosis virus (amv) (a mixture of subgroups a and b), rous-associated virus (rav)-2 (subgroup b), and carr-zilber strain of rous sarcoma virus (cz-rsv) (subgroup d) but not with prague strain of rsv (prc-rsv) (subgroup c) or ... | 1986 | 3015099 |
| d-galactosyltransferase and its endogenous substrates in chick embryo fibroblasts transformed by rous sarcoma virus. | udp-d-galactose: 2-acetamido-2-deoxy-beta-d-glucopyranosyl 4-beta-d-galactosyltransferase (galtase) activity was purified, from primary chick embryo fibroblast (cef) transformed by a temperature-sensitive, rous sarcoma virus mutant (cef-rsv), by chromatography on an affinity resin prepared with monoclonal antibodies to galtase. cellular glycopeptides from cef, as well as cef-rsv, maintained at permissive (37 degrees) [cef-rsf (37 degrees)] and nonpermissive temperatures (41 degrees) [cef-rsv (41 ... | 1986 | 3015407 |
| transplantable primate tumors induced by rous sarcoma virus. i. induction of tumors transplantable into young marmosets. | the tumors induced in white-lipped marmosets (saguinus fuscicollis, s. nigricollis) by rous sarcoma virus (rsv) of chicken origin (rsv-sr) were not transplantable to allogeneic hosts. in contrast, rsv rescued from these tumors (rsv-m) induced sarcomas that were transplantable to young but not to adult marmosets. the tumors induced by rsv-m and the transplants rapidly enlarged, metastasized to various organs, and killed the recipients 29-59 days post inoculation. cell lines were readily establish ... | 1986 | 3016397 |
| detection of transcription and translation in situ with biotinylated molecular probes in cells transfected with recombinant dna plasmids. | the efficiency of transfection and subsequent expression of recombinant dna plasmids in monolayers of cv-1 monkey kidney cells was analyzed by immunoperoxidase and in situ hybridization with biotin-nucleotide-labeled dna molecular probes. two recombinant plasmids were used for transfection. both contained the 3' long terminal repeat (ltr) of rous sarcoma virus (rsv) as the transcriptional promoter, but two different coding sequences were employed [bacterial chloramphenicol acetyltransferase (prs ... | 1986 | 3017147 |
| 31p nmr spectra of rodent and avian fibroblasts transformed by rous or kirsten sarcoma viruses. | 31p nmr spectra of normal rodent and avian fibroblasts were compared to those of the same cells transformed either by the rous sarcoma virus (rsv) or by the kirsten sarcoma virus (ki-msv). under physiological conditions, the spectra of living or perchloric acid extracted chicken embryo fibroblasts, rat cell line fr3t3 and mouse cell line c127 did not differ from those of their counterparts transformed by rsv or ki-msv. however, in the case of fr3t3 cells, on shifting from 37 degrees c to 20 degr ... | 1986 | 3017336 |
| cleavage of vimentin in dense cell cultures. inhibition upon transformation by type 5 adenovirus. | the analysis on two-dimensional isoelectric focusing and sds polyacrylamide gels (2d gels) of the triton x-100 and high salt-insoluble fraction of fibroblast cell lines, certain epithelial cell lines and granulosa cells revealed various amounts of a vimetin cleavage product, with a more basic pi and with a mw (1,500-2,000) lower than that of intact vimentin. this cleavage product of vimentin which constituted as much as 30% of the total vimentin in an established rat embryo fibroblast cell line ... | 1986 | 3017738 |
| simultaneous infection with bordetella pertussis and respiratory syncytial virus in hospitalized children. | we compared three groups of hospitalized children with bordetella pertussis infection, respiratory syncytial virus (rsv) infection and dual b. pertussis/rsv infections in an effort to establish clinical and laboratory criteria by which to distinguish children with dual infections from children infected with either organism alone. the groups were compared for admission laboratory data, history of present illness, perinatal history and immunization history. children with pertussis were more likely ... | 1986 | 2876415 |
| structural and functional motifs of the rous sarcoma virus src protein. | site-directed mutagenesis techniques have been utilized to define important structural and functional domains within the rsv src gene product, pp60src. deletion mutations within the amino terminal one-half of the src gene which impinge upon a region of the src protein delineated by amino acid residues 143 to 169 yielded transformation defective viruses. src proteins encoded by such rsv mutants exhibited diminished tyrosine protein kinase activity in vitro and only slightly reduced levels of in v ... | 1986 | 2851527 |
| interleukin 1 and interleukin 1 inhibitor production by human macrophages exposed to influenza virus or respiratory syncytial virus. respiratory syncytial virus is a potent inducer of inhibitor activity. | respiratory viral infections are commonly associated with altered immune responses, such as proliferative responses to mitogens and antigens. to examine potential mechanisms, we examined production of il-1 and il-1 inhibitors by purified human peripheral blood-derived macrophages exposed to influenza virus or respiratory syncytial virus (rsv). il-1 and il-1 inhibitor activities in supernatant fluids from macrophages exposed to the viruses 24 h previously were measured using the standard mouse th ... | 1986 | 3485170 |
| cytotoxic t cell lysis of h-2-negative murine sarcoma cells. | the doh cell line was established from c3h.oh (h-2kd,dk) embryonic fibroblasts transformed with rous sarcoma virus (rsv) in vitro. when injected into syngeneic mice, doh cells were very weakly tumorigenic and induced a cytotoxic immune response. cytotoxic t lymphocytes (ctl) specifically lysed doh cells but not other rsv-induced sarcoma cells, which shared the h-2kd or h-2dk antigen, respectively, with doh. serological and immunochemical analysis of h-2 antigens subsequently showed that doh sarc ... | 1986 | 3486129 |
| passively acquired antibodies to respiratory syncytial virus impair the secondary cytotoxic t-cell response in the neonatal mouse. | passively acquired antibody has been known since the 1940s to impair the secondary antibody response to the homologous antigen. however, the effect of passive immunity on the t-cell response is largely unknown. the results presented here demonstrate that monoclonal antibodies (mabs) to respiratory syncytial virus (rsv), transferred in the mother's milk or injected directly, can reduce the generation of rsv-specific cytotoxic t-cell (tc) precursors by the neonatal mouse; the development of influe ... | 1986 | 3489669 |
| rice stripe virus: prototype of a new group of viruses that replicate in plants and insects. | the unusual filamentous particles of rice stripe virus contain four species of single-stranded rna. similar filamentous particles are common in other planthopper-borne viruses of the rice stripe virus group, of which a type virus, rice stripe virus, is reviewed. | 1986 | 2856619 |
| neutralizing activity in human milk fractions against respiratory syncytial virus. | neutralizing activity against respiratory syncytial virus (rsv) was measured in milk samples from 17 healthy women whose infants had an acute infection with respiratory syncytial virus (rsv) and from 27 women with healthy infants. all milk samples were obtained 2-8 months post partum. neutralizing activity was detected in 36 samples. no major difference in neutralizing titers was observed between the two groups, and the titers were low. rsv-specific iga was found in two samples, and rsv-specific ... | 1986 | 3564936 |
| genetic resistance to fowl cholera is linked to the major histocompatibility complex. | chickens of the iowa state s1 line have been selected for ability to regress rous sarcoma virus-induced (rsv) tumors, humoral immune response to gat (ir-gat), and erythrocyte antigen b. sublines homozygous at the major histocompatibility complex (mhc), as well as f1 heterozygotes and f2 segregants, were tested for resistance to fowl cholera by challenge with pasteurella multocida strain x73. control of the response at high doses was associated in a preliminary study with ir-gat and response to r ... | 1987 | 3570380 |
| respiratory syncytial virus (rsv) infection rate in personnel caring for children with rsv infections. routine isolation procedure vs routine procedure supplemented by use of masks and goggles. | respiratory syncytial virus (rsv) infections in hospitalized children were identified by indirect fluorescent antibody technique. patients with rsv infections were assigned to one of two isolation categories. in one category, the health care workers entering the child's room did not wear masks and goggles; in the other category, the workers did wear masks and goggles. the rsv illness rate in health care workers using masks and goggles was 5%, but the rate for those not using masks and goggles wa ... | 1987 | 3578197 |
| prevention of nosocomial respiratory syncytial virus infections through compliance with glove and gown isolation precautions. | to determine whether increased compliance with a policy of glove and gown isolation precautions could reduce the high rate of nosocomial respiratory syncytial virus (rsv) infection on an infant and toddler ward, we conducted a longitudinal intervention trial during three rsv seasons, from 1982 to 1985, with an intervention to increase compliance introduced midway through the second season. the risk of acquiring rsv infection in the hospital was adjusted for the intensity of nosocomial exposure t ... | 1987 | 3600729 |
| identification of an amino terminal domain required for the transforming activity of the rous sarcoma virus src protein. | transformation of chicken cells by rous sarcoma virus (rsv) requires the functional expression of the viral src protein, a tyrosine protein kinase, pp60src. variants of rsv containing deletions within the amino terminal one-third of the src protein have been identified that exhibit either temperature-sensitive or transformation-defective phenotype when used to infect chicken embryo cells. to define the regions within the amino terminal portion of pp60src that influence morphological transformati ... | 1987 | 3660586 |
| vaccines for respiratory disease in cattle. | respiratory disease is one of the most serious disease complexes affecting beef cattle production. for example, it is claimed to cost the uk industry about 70 million pounds per year. it is usually associated with young cattle and can occur in a variety of situations. it is a good example of multifactorial disease in that its aetiology involves both infection by a variety of microorganisms and a number of environmental factors. several distinct syndromes occur and a number of microorganisms are ... | 1987 | 3673201 |
| growth-inhibitory protein present in rabbit serum, which is more effective on tumorigenic rat liver epithelial cells than on non-tumorigenic ones: its species, and mode of existence. | we have previously reported that in culture, rabbit serum inhibits the growth of the epithelial cell line from buffalo rat liver (brl) lower than that of the tumorigenic one transformed by rous sarcoma virus (rsv-brl). here, the serum was fractionated by several different methods. the findings are: 1) the growth inhibitor present (gi) existed as large complexes with non-inhibitory proteins; 2) the complexes were dissociated by 1 m nacl plus 6 m urea; 3) the dissociated gi did not pass through me ... | 1987 | 3689392 |
| cytotoxic t cell specificity for respiratory syncytial virus proteins: fusion protein is an important target antigen. | we examined the specificity of balb/c cytotoxic t (tc) cells for respiratory syncytial virus (rsv) components, using recombinant vaccinia viruses (vv) coding for several individual rsv proteins. we found that immunization with the different vvs yielded the following tc memory cell populations: high levels of rsv-specific tc cells were induced with the fusion protein vv, but low levels were induced with vv coding for the rsv nucleoprotein. tc cell recognition of attachment glycoprotein, part of t ... | 1987 | 3497222 |
| lymphocyte transformation response of calves to respiratory syncytial virus. | virus-specific cell-mediated immunity, as determined by in vitro lymphocyte transformation (lt), was demonstrated in calves following infection and vaccination with respiratory syncytial virus (rsv). after experimental infection, 4 of 6 gnotobiotic calves and 6 of 21 conventional calves developed a significant lt response to rsv. by means of a whole blood assay, the lt responses of calves were examined after vaccination with an inactivated vaccine, which consisted of glutaraldehyde-fixed bovine ... | 1987 | 3498008 |
| in vitro and in vivo stimulation of murine lymphocytes by human respiratory syncytial virus strains. | respiratory syncytial virus (rsv) strains of subtype a (a2, wv9894, and wv12138) and of subtype b (wv1293, wv4843, and wv6873) are mitogenic in vitro for unprimed balb/c spleen cells. the virus also triggered splenocytes in vitro to secrete immunoglobulins. plaque-purified and uv-irradiated materials of both rsv subtypes produced comparable levels of dna synthesis. infectious materials of both subtypes also induced pronounced responses. lymphocyte activation with uv-inactivated rsv strain a2 was ... | 1987 | 3498209 |
| clearance of persistent respiratory syncytial virus infections in immunodeficient mice following transfer of primed t cells. | little is known of the role of t-cell mediated immune responses in the clearance and pathogenesis of respiratory syncytial virus (rsv) infection. in this study, we established persistent pulmonary rsv infections in athymic nu/nu balb/c mice or immunodeficient irradiated balb/c mice, and examined the patterns of virus clearance following adoptive transfer of splenic memory t cells. primed t cells transferred between day 5 and day 8 of infection will clear lung rsv from both nu/nu mice and irradia ... | 1987 | 3498683 |
| respiratory syncytial virus infections in sr croatia, yugoslavia. | the prevalence of respiratory syncytial virus (rsv) infection was studied during three consecutive annual outbreaks (1983-1986) in sr croatia, yugoslavia. a total of 1,238 subjects were examined, using rsv isolation and immunofluorescent (dtfa) methods, with 1,042 showing the signs of respiratory infection and 207 of these having a positive rsv finding. generally, the prevalence of mild upper respiratory infection (urti) was 18%, reaching a peak of 30% at 1 year of age. the prevalence of severe ... | 1987 | 3499590 |
| total and specific ige responses in the acute and recovery phases of respiratory infections in children. | total ige and specific ige antibodies to common allergens were determined by the enzyme-linked immunosorbent assay in children with mycoplasma pneumoniae or respiratory syncytial virus (rsv) infections. the decrease of total ige from the acute to convalescent phase was observed in 105 of 134 (78.4%) children with m. pneumoniae infection and in 40 of 49 (81.6%) of those with rsv infection. similarly, many patients showed a decrease of the responses of specific ige in the convalescent phase of the ... | 1987 | 3505529 |
| relation of serum antibody to glycoproteins of respiratory syncytial virus with immunity to infection in children. | immunity in relation to passively transferred maternal and naturally-induced serum antibody to the viral proteins was determined in 34 children who were followed from birth through three years of age for respiratory syncytial virus infection (rsv). sera were tested by immunoglobulin class-specific enzyme-linked immunosorbent assay using the attachment and fusion proteins of the long strain. the basis for immunity for maternal antibody in primary infection was assessed by a comparison of the dist ... | 1987 | 3509676 |
| purification and properties of a non-histone chromatin protein with a molecular weight of 38,000 daltons specific for transformed rat cells. | non-histone chromatin proteins prepared from a normal rat cell line (no. 7) and the cells transformed with rous sarcoma virus (rsv) (s7-1) were compared by means of reverse-phase high performance liquid chromatography (reverse-phase hplc), followed by sds-polyacrylamide gel electrophoresis (sds-page). the results revealed that several proteins were specifically present in the transformed cell chromatin. a specific non-histone chromatin protein with a molecular weight of 38,000 daltons, 38k prote ... | 1987 | 2827654 |
| transformation of rat liver cell line by rous sarcoma virus causes loss of cell surface fibronectin, accompanied with secretion of metallo-proteinase that preferentially digests the fibronectin. | an epithelial cell line derived from the liver of a normal buffalo rat (brl) was transformed by rous sarcoma virus (rsv). the rsv-transformed cells were separated into five clones (rsv-brl1 through 5), which were morphologically different. rsv-brl cells exhibited the following characteristics distinct from those of brl cells: tumorigenicity, irregular cell arrangement, loose intercellular junction, growth in soft agar (anchorage-independent growth) except for rsv-brl3 and 5, and loss of cell sur ... | 1987 | 2828345 |
| restriction analysis of additional "non-producing" cell clones obtained after transformation of japanese quail fibroblasts with viruses rescued from cryptovirogenic h-19 cells. | the proviral structure of two transforming viruses rescued from cryptovirogenic h-19 hamster cells harbouring the ltr, v-src, ltr proviral structure was analysed by restriction mapping. it was established that the rescued g8 virus keeps the same restriction pattern as that observed in the cryptic h-19 provirus and that it is integrated into the cell genome at a different position characteristic of each "non-producing" (np) cell clone. in these features it corresponds to the previously described ... | 1987 | 2830149 |
| polyphosphoinositide metabolism is rapidly stimulated by activation of a temperature-sensitive mutant of rous sarcoma virus in rat fibroblasts. | we have examined polyphosphoinositide turnover in a rat-1 fibroblast line infected with a temperature-sensitive mutant (ts la24) of the rous sarcoma virus (rsv). when ts la24-infected cells are shifted from the non-permissive to the permissive temperature, a rapid and sustained activation of phospholipase c (plc) is observed. normal and wild-type rsv-infected rat-1 cells do not show any plc activation upon temperature shiftdown. pre-treatment of ts la24-infected fibroblasts with tetrodotoxin (a ... | 1987 | 2830581 |
| cell-mediated cytotoxic response to respiratory syncytial virus in infants with bronchiolitis. | a cell-mediated cytotoxic response to respiratory syncytial (rsv) was demonstrated in the peripheral blood of 4 of 22 infants with acute bronchiolitis. these 4 infants were aged 3 weeks to 3 months. no such response was found in infants older than 4 months. all 4 infants with a positive response had mild infections. | 1987 | 2888988 |
| rapid detection of respiratory syncytial virus in clinical specimens. | results of the direct immunofluorescence (if) test in 152 clinical specimens (throat swabs) were compared with those of respiratory syncytial virus (rsv) isolation. the prevalence of rsv infections in the upper and lower respiratory tract was high especially in infants until 12 months of age. the average rsv isolation rate was 18.42%, whereas the virus antigen detection was positive in 19.74% of cases. the agreement between virus isolation and direct if was 92.1%, the sensitivity of if being 82. ... | 1987 | 2891280 |
| tissue tropism and temporal expression of rous sarcoma virus in embryonic avian limb in ovo. | embryonic avian tissue is resistant to the transforming potential of rous sarcoma virus (rsv) in ovo. analysis of the pattern of host-viral interactions in the first semester of chick development has demonstrated that rsv is first expressed in a limited population of muscle precursor cells and proceeds to spread throughout the developing dorsal and ventral limb musculature. the number of non-muscle cells participating in the infection is initially low but gradually increases as development conti ... | 1987 | 2835735 |
| elevated phosphatidylinositol kinase activity in rous sarcoma virus-transformed cells. lack of evidence for enzyme translocation. | phosphatidylinositol kinase (e.c. 2.7.1.67) activity of rat fibroblasts transformed by rous sarcoma virus (rsv) was measured and compared with immunoprecipitated protein tyrosine kinase activity associated with pp60v-src. both enzyme activities were elevated in the particulate fractions from wild-type rsv-transformed cells and cells transformed by a temperature-sensitive mutant of rsv when grown at the permissive temperature. the presence of the non-ionic detergent nonidet p-40 in the phosphatid ... | 1987 | 2822141 |
| growth inhibitors in serum, platelets, and normal and malignant tissues. | the non-malignant epithelial cell line from buffalo rat liver (brl) and its malignant transformant obtained by rous sarcoma virus infection (rsv-brl) were used as the indicators. a survey was made in animals for growth inhibitors which could inhibit the growth of brl, but not the growth of rsv-brl in culture, under the consideration that the loss of the sensitivity to such inhibitors might be the most dominant cause for acquisition of tumorigenicity. from human platelets, a growth inhibitor was ... | 1987 | 2823546 |
| the methylation state of the proviruses in avian sarcoma virus transformed chick and rat cells. | the endogenous viruses in the avian cells are not completely methylated, nor are the schmidt-ruppin rsv-d (srd) proviruses in the infected cells completely unmethylated. avian sarcoma proviruses integrated in rat transformed cloned cells are heavily methylated. in these cells, a region in the 3' end of the env gene is unmethylated in all the src-containing proviruses but not in the transformed defective (td) proviruses. a possible role for the hypomethylation of the 3' end of the env region is p ... | 1987 | 2823897 |
| surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on virus interference. | during the 7-year period from september 1978 to august 1985, smear specimens of nasopharyngeal secretions from 3132 patients mainly hospitalized children, taken in different regions in norway, were examined for respiratory viruses by the rapid immunofluorescence (if) technique. a positive diagnosis for respiratory syncytial virus (rsv), parainfluenza virus type 1, 2 and 3 or influenza a and b virus was made for 896 patients (29%). the greatest prevalence for all these viruses was observed during ... | 1987 | 2824225 |
| [expression of a cross-reactive antigen on the surface of human epidermoid carcinomas overproducing egf-receptor shared with rsv-induced tumors]. | analysis based on the complement-dependent cytotoxicity (cdc) assays using syngeneic antiserum against a rous sarcoma virus(rsv)-induced mouse tumor(csa1m) showed that a cross-reactive antigen with a common tumor-associated cell surface antigen(tasa) of rsv-induced mouse tumors was shared with two human tumors a431 and mda-468 overexpressing epidermal growth factor receptor(egfr). the tasa, however, was not expressed on four human choriocarcinomas, a human lung cancer a2182, and human embryo fib ... | 1987 | 2824311 |
| [representation of the leader region of rous sarcoma virus genome rna in double-stranded rna produced in virus-transformed cells]. | restriction fragments of recombinant plasmids containing a proviral sequence of rous sarcoma virus (rsv) were southern hybridized with double-stranded (ds) rna isolated from the cells transformed with rsv. hybridization data show that the major subpopulation of dsrna molecules is homologous to the 5'-end region of the viral genome including the leader sequence. we have analysed the rnas of rsv-transformed cells by the northern procedure hybridizing them with the proviral fragment containing doub ... | 1987 | 2824985 |
| genetic associations of body weight and immune response with the major histocompatibility complex in white leghorn chicks. | survival, body weight, and humoral immune response to sheep red blood cells (srbc) were measured in 256 chicks of the iowa state university s1 leghorn line. the chicks were produced from breeders selected for serological ea-b blood type (b1 or b19), high or low humoral immune response to the amino acid polymer, glutamic acid-alanine-tyrosine (ir-gat), and response to rous sarcoma virus-induced (rsv) tumors (progression or regression). chicks were hatched from sublines representing all eight trip ... | 1987 | 2825154 |
| epidemiology of viral infections in singapore. | as singapore is a densely populated island, and also a major air and sea port, the importation and dissemination of viral infections is facilitated. respiratory viral infections have the highest prevalence rates, influenza and respiratory syncytial virus (rsv) being the most important ones. seasonal variation occurs with influenza, rsv and parainfluenza virus type 1 infections. the age distribution and clinical picture associated with infections due to the various respiratory viruses are similar ... | 1987 | 2825585 |
| reduced expression of mhc class i antigens in chicken fibroblasts transformed by rous sarcoma virus. | monoclonal monomorphic as well as polyclonal antibodies against h-b class i (b-f) antigen were used to evaluate the expression of these molecules on normal and rsv-transformed chick embryo fibroblasts. the results indicate that chicken fibroblasts transformed in vitro by the schmidt-ruppin strain rous sarcoma virus of subgroup b (sr-rsv-b) present reduced h-b class i antigen expression as compared to uninfected cells of the same inbred strain. this quantitative reduction was observed at the cell ... | 1987 | 2826262 |
| specific nuclear proteins interact with the rous sarcoma virus internal enhancer and share a common element with the enhancer located in the long terminal repeat of the virus. | we have documented that the rous sarcoma virus (rsv) internal enhancer functions in the nontransformed baby hamster kidney (bhk) cell line. the sequences within this region were assayed for their ability to bind to specific factors present in bhk nuclear extracts using the gel retardation assay and dnase i footprinting. at least two sequences within the internal enhancer which can specifically bind nuclear factors in vitro have been identified. these regions are located between nucleotides 813-8 ... | 1987 | 2827114 |
| pulmonary infections with respiratory syncytial virus and the parainfluenza viruses. | respiratory syncytial virus (rsv) and the parainfluenza viruses (pivs) are the most important causes of acute lower respiratory illness (lri) in infants and children under 6 years of age. these enveloped viruses are members of the paramyxovirus family. they infect cells in the epithelium lining the trachea and intrapulmonary airways, and cause croup, bronchitis, bronchiolitis, and bronchopneumonia. rsv causes annual midwinter to early spring outbreaks of respiratory disease in temperate climates ... | 1987 | 2827280 |
| clinical review of ribavirin. | the recent approval of ribavirin aerosol for the treatment of severe respiratory syncytial virus (rsv) in infants and young children is a significant addition to the antiviral drugs available today. when administered as an aerosolized form by face mask or mist tent for 20 to 21 hours per day, ribavirin effectively decreases the symptoms of rsv infection and the shedding of rsv virus. studies of other viral infections such as viral hepatitis, influenza a and b, lassa fever, genital herpes, and he ... | 1987 | 3298116 |
| immunological differences between the envelope glycoproteins of two strains of human respiratory syncytial virus. | the envelope glycoproteins of two distinct strains of respiratory syncytial virus (rsv) (long and 18537 strains) were purified by affinity chromatography and characterized by immunological methods. the fusion (f) proteins from the two strains were similar in molecular weight by gel electrophoresis and were very closely related immunologically. rabbit antisera to either f protein reacted with near equivalent titres with the heterologous f protein by western blot, enzyme-linked immunoassay (eia), ... | 1987 | 3302101 |
| antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups a and b: evaluation of the contributions of f and g glycoproteins to immunity. | the degree of antigenic relatedness between human respiratory syncytial virus (rsv) subgroups a and b was estimated from antibody responses induced in cotton rats by respiratory tract infection with rsv. glycoprotein-specific enzyme-linked immunosorbent assays of antibody responses induced by rsv infection demonstrated that the f glycoproteins of subgroups a and b were antigenically closely related (relatedness, r approximately 50%), whereas the g glycoproteins were only distantly related (r app ... | 1987 | 3305988 |
| application of pooled monoclonal antibodies for 1-hr detection of respiratory syncytial virus antigen in clinical specimens. | a fluorescein isothiocyanate-conjugated pool of monoclonal antibodies (moab) to respiratory syncytial virus (rsv) was prospectively evaluated for its utility as a direct, 1-hr test for the diagnosis of rsv infection. direct nasopharyngeal swab smears collected from 109 infants and children with acute respiratory illnesses were studied and compared with results obtained by indirect immunofluorescence using bovine polyclonal anti-rsv antibody on eluted cells derived from pooled nasopharyngeal and ... | 1987 | 3308304 |
| microplaque immunoperoxidase detection of infectious respiratory syncytial virus in the lungs of infected mice. | a rapid microplaque technique was developed for the detection of infectious respiratory syncytial virus (rsv) in the lungs of infected mice. infected lung homogenates were titrated on microwell hep-2 monolayers and incubated for 24 or 48 h. the microwells were then fixed with 4% formaldehyde in saline, or methanol containing 0.5% hydrogen peroxide. 24-h single cell infectious foci and 48-h microplaques were detected by an indirect immunoperoxidase (iip) assay using monoclonal antibodies specific ... | 1987 | 3312262 |
| comparison of nasopharyngeal washings and swab specimens for diagnosis of respiratory syncytial virus by eia, fat, and cell culture. | respiratory secretions for viral diagnosis are often collected with nasopharyngeal (np) swabs, although many laboratories recommend np aspirates or washings. we compared results using np washings and np swabs in three diagnostic rsv tests, a rapid rsv eia antigen test (abbott laboratories), an indirect fluorescent antibody test (fat) with rabbit antiserum, and virus culture (hep-2 cells). paired samples were collected from 121 children with suspected rsv bronchiolitis or pneumonia. a minitip swa ... | 1987 | 3322655 |
| comparison of two enzyme-linked immunosorbent assay (eia) kits with immunofluorescence and isolation in cell culture for detection of respiratory syncytial virus (rsv). | rapid diagnosis of respiratory syncytial virus (rsv) infections is based upon detection of viral antigen in cells obtained from the respiratory tract and usually employs immunofluorescence (if) reactions or enzyme-linked immunosorbent assays (eia). the pathfinder eia kit (kallestad diagnostics) was compared with the abbott eia kit by evaluating each against isolation of rsv in cell culture and detection of antigen by if. the pathfinder kit identified 116 of 129 culture-positive and 72 of 90 cult ... | 1987 | 3329591 |
| ribavirin aerosol treatment of serious respiratory syncytial virus infection in infants. | bronchiolitis is one of the most serious pulmonary infections commonly caused by respiratory syncytial virus (rsv). rsv disease occurs in yearly epidemics and is most severe in children 1 year of age or younger. approximately 1 in 50 to 1 in 100 infants are hospitalized after their first infection, and mortality fluctuates between 0.5 and 5.0 per cent. patients with underlying conditions such as congenital heart disease and bronchopulmonary dysplasia are at higher risk for morbidity and mortalit ... | 1987 | 3332796 |
| virus infections in childhood malignant disease. | the viruses isolated during infective episodes over a 5-year period of 93 children with acute lymphoblastic leukemia (all) and 107 children with other malignancies have been studied. viruses were identified by the fluorescent antibody technique (fat), electron microscopy, and culture. viruses were isolated on 204 occasions. rhinovirus and rsv occurred significantly more often in the patients with all, and adenovirus more commonly in the solid tumor patients. viruses were responsible for 4 of the ... | 1987 | 3152935 |
| characterization of rous sarcoma virus sequences essential for viral gene expression. | using the escherichia coli lacz gene product beta-galactosidase as an indicator of gene expression, we analyzed sequences that are required for expression of the rous sarcoma virus (rsv) genome in avian cells. the rsv long terminal repeat (ltr) and leader region were sufficient to direct the synthesis of high levels of enzymatically active gag-lacz fusion proteins. a portion of u3 greater than 140 nucleotides upstream from the cap site was essential for gene expression. this element functioned i ... | 1987 | 3029412 |
| at least two nuclear proteins bind specifically to the rous sarcoma virus long terminal repeat enhancer. | we used the sensitive gel electrophoresis dna-binding assay and dnase i footprinting to detect at least two protein factors (efi and efii) that bound specifically to the rous sarcoma virus (rsv) enhancer in vitro. these factors were differentially extracted from quail cell nuclei, recognized different nucleotide sequences in the u3 region of the rsv long terminal repeat, and appeared to bind preferentially to opposite dna strands as monitored by the dnase i protection assay. the efi- and efii-pr ... | 1987 | 3029568 |
| transformation by rous sarcoma virus induces a novel gene with homology to a mitogenic platelet protein. | a cdna clone, designated 9e3, was isolated from a chick embryo fibroblast (cef) cdna library. 9e3 mrna was 20-fold higher in cef following transformation by rous sarcoma virus because of increased transcription rate. in cef infected with temperature-sensitive mutants, increased 9e3 mrna was found within 2 hr of a shift to permissive temperature. nucleotide sequence and in vitro translation results indicate that 9e3 mrna encodes an 11 kd polypeptide that is homologous to human connective tissue a ... | 1987 | 3032449 |
| differentiation of retrovirus-infected avian neuroretina cells. | the effects of oncogenic retroviruses on the expression of differentiation markers were studied in monolayer cultures of chick and quail embryo neuroretinas. transformation by rous sarcoma virus (rsv) did not affect the appearance of synapses, and the expression of glutamic acid decarboxylase was stimulated by pp60v-src, the product of the src gene. quail embryo neuroretina cells transformed by mill hill 2 (which contains the two oncogenes v-mil and v-myc) were induced to proliferate into perman ... | 1987 | 3032462 |
| morphf mutants of rous sarcoma virus: nucleotide sequencing analysis suggests that a class of morphf mutants was generated through splicing of a cryptic intron. | the nature of the lesions involved in producing the fusiform phenotype of three mutants (wo101, wo201, and tsst529) of the schmidt-ruppin a strain of rous sarcoma virus (rsv) was determined by molecular cloning and dna sequencing. wo101 and wo201 contained an in-frame deletion of the v-src region coding for amino acids 116 to 140 of p60v-src. the deleted segment was flanked by consensus splice donor and acceptor sequences and contained an appropriately positioned branchpoint acceptor consensus s ... | 1987 | 3033320 |
| integration and expression of provirus in human cells transformed by avian sarcoma virus. | previously, human diploid fibroblasts from some donors infected in vitro by avian sarcoma virus (asv) were transformed and found, by electron microscopy, to produce small numbers of virus particles that were infectious by bioassay; also, a line of human osteosarcoma cells infected with asv developed additional characteristics of transformation and released a small number of infectious virus particles. in this study the complete proviral sequence was shown to be integrated in the genome of these ... | 1987 | 3033382 |
| interferon-gamma inhibits cell replication, but not pp60src activity of rsv-transformed fibroblasts. | the present study analyzes the effect of murine ifn-gamma on dna synthesis, ornithine decarboxylase activity and phosphorylation of pp60src of rouse sarcoma virus transformed cells. when natural or recombinant ifn-gamma was added to quiescent sr-balb or l1210 cells, stimulated with fetal calf serum, only the highest ifn-gamma concentrations (2000 u/ml) inhibited dna synthesis of both tumor lines. by contrast, lower ifn-concentrations (20-200 u/ml) inhibited the dna synthesis of l1210 but not of ... | 1987 | 3033451 |
| rous sarcoma virus is integrated but not expressed in chicken early embryonic cells. | we have developed a protocol that allows us to infect chicken early embryonic (cee) cells with high efficiency. this was achieved by exposing the cee cells to a semicontinuous dose of rous sarcoma virus (rsv) for a period of 20 hr. southern blot analysis indicated that an average of one proviral copy is integrated per embryonic cell. however, there was no production of infectious viral particles by the cells containing the proviral genome, although low levels of full-length genomic rna could be ... | 1987 | 3033663 |