Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| a generalized method of subcloning dna fragments by restriction site reconstruction: application to sequencing the amino-terminal coding region of the transforming gene of gazdar murine sarcoma virus. | the technique of restriction site reconstruction was generalized so as to allow the subcloning of any dna fragment and its subsequent reexcision with ecori, xbai, xhoi or hindiii. after excision, the 3' terminus of each strand will be derived from the starting nucleic acid, permitting the use of such fragments as primers for nucleotide sequencing by primer extension methods. the technique was used to subclone a 56 base pair bstni-ddei fragment of moloney murine sarcoma virus (mo-msv) as a unique ... | 1982 | 6281735 |
| candidate product of the fbj murine osteosarcoma virus oncogene: characterization of a 55,000-dalton phosphoprotein. | sera from rat bearing tumors induced by inoculation of fbj murine osteogenic sarcoma virus (fbj-msv) nonproducer rat cells precipitate two proteins with molecular weights of 55,000 (p55) and 39,000 (p39) from fbj-msv-transformed cells. these proteins cannot be precipitated from uninfected cells or cells transformed by other strains of murine sarcoma virus, nor can they be precipitated by sera specific for the viral structural proteins. a methionine tryptic peptide mapping analysis showed that p5 ... | 1982 | 6283132 |
| identification of a precursor in the biosynthesis of the p21 transforming protein of harvey murine sarcoma virus. | the p21 transforming protein coded for by the v-ras gene of harvey murine sarcoma virus (ha-musv) migrates as a doublet band between 21,000 and 23,000 daltons during sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the lower band of the doublet is designated p21, and the upper band is designated pp21 since it comigrates with the phosphorylated form of p21. by pulse-labeling with [35s] methionine, we detected a p21 precursor, pro-p21, which migrated as if it was approximately 1,000 dalt ... | 1982 | 6283143 |
| tumorigenic transformation of mammalian cells induced by a normal human gene homologous to the oncogene of harvey murine sarcoma virus. | a normal human gene homologous to the p21 ras oncogene of harvey murine sarcoma virus induced oncogenic transformation and high p21 ras levels in murine fibroblasts when this gene was ligated to a control element (the long terminal repeat) from a murine or feline retrovirus. these results indicate that high levels of a gene product encoded by a normal human oncogene can induce tumorigenic transformation. | 1982 | 6283358 |
| t24 human bladder carcinoma oncogene is an activated form of the normal human homologue of balb- and harvey-msv transforming genes. | a transforming gene isolated from t24 human bladder carcinoma cells is closely related to the balb murine sarcoma virus (msv) onc gene (v-bas). this transforming gene is localized to a 4.6 kilobase pair (kbp) region and is expressed as a 1.2-kbp polyadenylated transcript, which contains v-bas related sequences. moreover, antisera known to detect the immunologically related onc gene products of balb- and harvey-msvs recognized elevated levels of a related protein in t24 cells. the normal human ho ... | 1982 | 6283384 |
| expression of cellular homologues of retroviral onc genes in human hematopoietic cells. | total cellular poly(a)-enriched rna from a variety of fresh human leukemic blood cells and hematopoietic cell lines was analyzed for homology with molecularly cloned dna probes containing the onc sequence of abelson murine leukemia virus (ab-mulv), harvey murine sarcoma virus (ha-musv), simian sarcoma virus (ssv), and avian myelocytomatosis virus strain mc29. results with the fresh blood cells paralleled those obtained with the cell lines. with ab-mulv and ha-musv, multiple rna bands were visual ... | 1982 | 6283530 |
| viral src gene products are related to the catalytic chain of mammalian camp-dependent protein kinase. | the transforming protein sequences translated from the rous avian and moloney murine sarcoma virus src genes are shown to be related to the catalytic chain of bovine camp-dependent protein kinase (atp:protein phosphotransferase, ec 2.7.1.37). the avian transforming protein, also a protein kinase, shows greatest homology with the bovine protein kinase in the carboxyl-terminal half, where the protein kinase activity is localized. moreover, lysine occurs in the inferred transforming protein sequenc ... | 1982 | 6283546 |
| induction of sarcomas in athymic mice. | during the course of serial passage of 50 human xenografts in the athymic mouse over a period of 5 years we have observed two cases of induction of sarcomas in the murine stromal tissue associated with the human xenografts. both times the growth of the murine sarcomas overtook that of the human xenograft. this change was monitored by analysis of the lactate dehydrogenase isozyme profile and histology of each passage of the human xenografts in the athymic mice. the two murine sarcomas were subseq ... | 1982 | 6283553 |
| nucleotide sequence analysis of the long terminal repeat of integrated simian sarcoma virus: evolutionary relationship with other mammalian retroviral long terminal repeats. | nucleotide sequence analysis of the long terminal repeat (ltr) of the integrated simian sarcoma virus showed that the simian sarcoma virus ltr comprised 504 nucleotides with an inverted repeat of seven bases at its 5' and 3' termini. at the site of simian sarcoma virus integration, cellular flanking sequences adjacent to the proviral ltr contained a direct repeat of four bases. a 13-base sequence after the 5' ltr was found to be complementary to prolyl trna, suggesting that trnapro may serve as ... | 1982 | 6284990 |
| balb- and harvey-murine sarcoma virus transformation of a novel lymphoid progenitor cell. | balb- and harvey-murine sarcoma viruses (msv) comprise a family of retroviruses whose mouse- and rat-derived onc genes are closely related. these viruses induce sarcomas and erythroleukemias in susceptible animals. an in vitro colony assay that detects transformation of lymphoid cells by abelson-murine leukemia virus was used to demonstrate that balb- and harvey-msv transform a novel hematopoietic cell both in culture and in vivo. bone marrow colony formation was sarcoma virus dependent, followe ... | 1982 | 6286838 |
| monoclonal antibodies to the p21 products of the transforming gene of harvey murine sarcoma virus and of the cellular ras gene family. | we have isolated eight rat lymphocyte-myeloma hybrid cell lines producing monoclonal antibodies that react with the 21,000-dalton transforming protein (p21) encoded by the v-ras gene of harvey murine sarcoma virus (ha-musv). these antibodies specifically immunoprecipitate both phosphorylated and non-phosphorylated forms of p21 from lysates of cells transformed by ha-musv. all eight react with the products of closely related ras genes expressed in cells transformed by two additional sarcoma virus ... | 1982 | 6287003 |
| cellular moloney murine sarcoma (c-mos) sequences are hypermethylated and transcriptionally silent in normal and transformed rodent cells. | moloney murine sarcoma virus carries an oncogenic sequence (v-mos) which is homologous to a single copy gene (c-mos) present in the normal cells of several vertebrate species. because of the possible significance of c-mos sequences in normal development and malignant transformation induced by physical or chemical agents, we have examined the state of integration, methylation, and transcriptional activity of c-mos sequences in a variety of normal rodent tissues, normal cell lines, or cell lines t ... | 1982 | 6287220 |
| human dna sequence homologous to the transforming gene (mos) of moloney murine sarcoma virus. | we describe the molecular cloning of a 9-kilo-base-pair bamhi fragment from human placental dna containing a sequence homologous to the transforming gene (v-mos) of moloney murine sarcoma virus. the dna sequence of the homologous region of human dna (termed humos) was resolved and compared to that of the mouse cellular homolog of v-mos (termed mumos) [van beveren, c., van straaten, f., galleshaw, j.a. & verma, i.m. (1981) cell 27, 97-108]. the humos gene contained an open reading frame of 346 co ... | 1982 | 6287464 |
| nucleotide sequence of the oncogene encoding the p21 transforming protein of kirsten murine sarcoma virus. | the transforming protein ofkirsten murine sarcoma virus (ki-musv) is a virally encoded 21-kilodalton protein called p21 kis. the sequences encoding p21 kis were genetically localized to a 1.3-kilobase segment near the 5' end of the viral genome by assaying the capacity of a series of defined deletion mutants of molecularly cloned ki-musv dna to induce focal transformation of mouse cells. nucleotide sequencing of a portion of this region has led to the identification of an open reading frame of 5 ... | 1982 | 6287573 |
| detection of a transforming gene product in cells transformed by moloney murine sarcoma virus. | we identified, in cells transformed by moloney murine sarcoma virus (m-musv clone 124), a protein encoded by the m-musv transforming gene, v-mos. an antiserum against a synthetic peptide corresponding to the c terminus of a protein predicted from the v-mos nucleotide sequence specifically recognizes a protein doublet of approximately 37,000 daltons from 35s-methionine-labeled m-musv 124-transformed producer cells. by peptide mapping, this protein is almost identical to the 37 kd in vitro transla ... | 1982 | 6288258 |
| murine xenotropic type c viruses. iv. replication and pathogenesis of ducks. | the xenotropic (x-tropic) mouse type c virus (mulv) and its pseudotype of murine sarcoma virus (msv) were inoculated into several fertilized developing pekin duck eggs. the development of the duck embryos was substantially reduced in those receiving the x-tropic viruses compared to eggs inoculated only with tissue culture medium. infections virus was isolated from some of the adult animals; in others, evidence for integrated virus sequences in the tissues was noted. no specific pathology was fou ... | 1982 | 6288852 |
| autocrine secretion of peptide growth factors by tumor cells. | transforming growth factors (tgf) are growth-promoting polypeptides that cause phenotypic transformation and anchorage-independent growth of normal cells. they have been isolated from several human and animal carcinoma and sarcoma cells. one tgf is sarcoma growth factor (sgf) which is released by murine sarcoma virus-transformed cells. whereas the tgf interacts with epidermal growth factor (egf) cell membrane receptors, it is not detectable in culture fluids from cells which contain high numbers ... | 1982 | 6289103 |
| human genome contains four genes homologous to transforming genes of harvey and kirsten murine sarcoma viruses. | harvey and kirsten murine sarcoma viruses each encode a structurally and functionally related 21-kilodalton protein (p21), which is the transforming protein of each virus. using probes from the 0.9-kilobase (kb) p21-coding region of each virus (called v-ha-ras and v-ki-ras, respectively), we have molecularly cloned from normal human genomic dna the sequences that hybridize to these probes. four evolutionarily divergent restriction endonuclease fragments were isolated. two hybridized preferential ... | 1982 | 6289320 |
| kirsten murine sarcoma virus-coded p21ras may act on multiple targets to effect pleiotropic changes in transformed cells. | 1982 | 6289520 | |
| comparison of complexes containing lysyl-trna synthetase from normal and virus-transformed cells. | we compared the lysyl-trna synthetases from normal (balb/3t3) and murine sarcoma virus-transformed (ka31) mouse fibroblasts. in agreement with several other reports of mammalian systems, the lysyl-trna synthetases from these cells occurred in very large postmicrosomal complexes as determined by gel filtration on agarose columns. arginyl-, isoleucyl-, methionyl-, phenylalanyl-, and tyrosyl-trna synthetases also occurred as part of a large complex or complexes. activity of glycyl- or leucyl-trna s ... | 1982 | 6290008 |
| molecular cloning and chromosomal mapping of a human locus related to the transforming gene of moloney murine sarcoma virus. | human dna was analyzed for the presence of sequences homologous to the transforming gene (v-mos) of moloney murine sarcoma virus. a single 2.5-kilobase pair (kbp) ecori-generated fragment of human dna was identified by using cloned v-mos as probe. this dna was molecularly cloned in a bacteriophage vector. by heteroduplex and restriction enzyme analyses, this human dna fragment, designated c-mos (human), contained a 0.65-kbp region of continuous homology with v-mos and was present as a single cop ... | 1982 | 6291031 |
| loss and restoration of glucagon receptors and responsiveness in a transformed kidney cell line. | a kidney cell line (mdck) retains an adenylate cyclase system sensitive to glucagon, vasopressin, isoproterenol and prostaglandin e1. the stimulatory effect of glucagon on camp production was selectively lost in a cloned line derived from mdck cells transformed by harvey murine sarcoma virus. sensitivity to glucagon was largely restored by treatment of the transformed cells with prostaglandin e1 or butyrate. loss and reappearance of glucagon receptors seemed to be responsible for the observation ... | 1982 | 6291963 |
| sarcoma growth factor and other transforming peptides produced by human cells: interactions with membrane receptors. | transforming growth factors (tgfs) stimulate cells to divide in monolayer cultures and to form colonies that grow progressively in soft agar. tgfs are a family of polypeptide hormones that, in vitro, confer on fibroblasts and epithelial cells properties associated with the transformed phenotype. they have been isolated from the supernatant fluids of several human and animal carcinoma and sarcoma cells. tgfs interact with epidermal growth factor (egf) membrane receptors. they are not detectable i ... | 1982 | 6292003 |
| characterization of xenotropic and dual-tropic type c retroviruses isolated from abelson tumour. | tumours induced in balb/c mice by abelson virus complex were found to contain a xenotropic virus (a-x-mulv) and an nb-tropic, dual-tropic virus (nbx), in addition to the moloney leukaemia virus (m-mulv) and the defective, transforming abelson virus genome. both a-x-mulv and nbx virus were presumably present in a genomically masked form and could be recovered only by co-cultivation of tumour cells with permissive cells. only about 0.87% and 0.13% of the viruses in the co-culture supernatant repre ... | 1982 | 6292337 |
| restriction endonuclease mapping of unintegrated proviral dna of kirsten murine sarcoma virus. | linear unintegrated kirsten murine sarcoma virus dna synthesized after acute infection of nih/3t3 cells or by detergent-disrupted virions, was studied by restriction enzyme cleavage and agarose gel electrophoresis. labelled dna (cdna) synthesized by reverse transcription of a virion rna template was used to detect viral dna sequences by filter hybridization. the sites of cleavage for eleven enzymes were located on the genome. hybridization studies using a cdna probe specific for the 3' end of th ... | 1982 | 6292347 |
| transfer of murine leukaemia and murine sarcoma virus genetic information by transfection with isolated metaphase chromosomes. | chromosome-mediated transfer of murine leukaemia (mulv) and murine sarcoma (musv) virus genetic information to uninfected recipient cells was investigated. metaphase chromosomes from akr mulv-infected sc-1 mouse cells were incubated with nih/3t3 cells. after several passages (1 to 3 weeks), infectious virions exhibiting reverse transcriptase activity and the characteristic host range of ecotropic, n-tropic akr virus appeared in the supernatant fluids of the treated cells. restriction endonucleas ... | 1982 | 6292350 |
| comparative biochemical properties of p21 ras molecules coded for by viral and cellular ras genes. | in earlier studies, we molecularly cloned a normal cellular gene, c-rash-1, homologous to the v-ras oncogene of harvey murine sarcoma virus (v-rash). by ligating a type c retroviral promotor to c-rash-1, we could transform nih 3t3 cells with the c-rash-1 gene. the transformed cells contained high levels of a p21 protein coded for by the c-rash-1 gene. in the current studies, we have purified extensively both v-rash p21 and c-rash p21 and compared the in vivo and in vitro biochemical properties o ... | 1982 | 6292515 |
| molecular cloning, genomic analysis, and biological properties of rat leukemia virus and the onc sequences of rasheed rat sarcoma virus. | rasheed rat sarcoma virus (rasv) has been shown to code for a protein of 29,000 mr not present in replication-competent rat type c helper virus (ralv)-infected cells. this protein is a fused gene product consisting of a portion of the ralv p15 gag protein and the transformation-specific 21,000 mr (p21) ras protein, which is also found in harvey murine sarcoma virus. we now report the molecular cloning of both the sd-1 (sprague-dawley) strain of ralv and the transforming ras sequences of rasv. he ... | 1982 | 6292516 |
| host-specific activation of transcription by tandem repeats from simian virus 40 and moloney murine sarcoma virus. | the simian virus (sv40) 72-base pair (bp) tandem repeated sequences have recently been shown to function as activators or enhancers of early viral transcription. a recombinant viral genome was recently constructed by inserting 72-bp tandem repeats from the moloney murine sarcoma virus (msv) in place of the 72-bp repeats of sv40. although this genome replicates in monkey kidney cells, its rate of large tumor antigen expression and replication is considerably slower than that of wild-type sv40. in ... | 1982 | 6292901 |
| genetic organization and cloning of kirsten murine sarcoma virus dna. | 1982 | 6295386 | |
| repeated isolation of unique qa2+ ia+ clonally derived cell lines from qa2- mice. | qa2+ tumor cell lines were previously isolated from individual balb/cby (qa2-) splenic lymphomas induced by murine sarcoma virus-murine leukemia virus-moloney (msv-mulv-m). two clonally derived cell lines, ora i-a and thorbly, and one noncloned cell line, boms, expressed qa2, but neither ly-1 nor ly-2 were detected. in order to determine whether eight cloned and two noncloned tumor cell lines all represented a unique population of transformed cells, the presence of a series of surface differenti ... | 1982 | 6979571 |
| comparative tryptic peptide analysis of candidate p85gag-mos of ts110 moloney murine sarcoma virus and p38-p23 mos gene-related proteins of wild-type virus. | 1982 | 6981878 | |
| mouse embryonic transforming growth factors related to those isolated from tumor cells. | growth factors with the two characteristic properties of sarcoma growth factor, the ability to stimulate anchorage-independent growth of normal mouse or rat fibroblasts and the ability to compete with 125i-labeled epidermal growth factor for receptor binding, can be isolated from 12- to 13-day-old normal mouse embryos of various strains. two size classes of the transforming factor from embryo cells can be isolated with apparent molecular weights of 20,000 and 10,000. the lower-molecular-weight f ... | 1982 | 6275982 |
| block in the expression of differentiation markers of rat thyroid epithelial cells by transformation with kirsten murine sarcoma virus. | well-differentiated epithelial cells, derived from primary cultures of normal rat thyroid glands (t-79 cells), as well as a cloned cell line also derived from normal rat thyroid glands (frt-l cells) were infected with kirsten murine sarcoma virus carrying outer coat of the helper kirsten murine leukemia virus. infected t-79 and frt-l cells changed morphologically and began to proliferate rapidly, suggesting malignant transformation by the virus. both cell lines can support the replication of bot ... | 1982 | 6275984 |
| transformation of mmc-e epithelial cells by acute 3611-msv: inhibition of collagen synthesis and induction of novel polypeptides. | mouse embryo epithelial cells mmc-e were transformed by novel fibrosarcoma-inducing murine sarcoma virus 3611-msv. the cells were analyzed for the production and deposition of pericellular glycoproteins by immunofluorescence and by radioactive metabolic and cell surface labeling techniques followed by analysis in polyacrylamide gels and fluorography. the pericellular fibronectin matrix was lost, but unlike in virus-transformed fibroblastic cells, the production of fibronectin was not affected. t ... | 1982 | 6302114 |
| activation of sv40 genome by 72-base pair tandem repeats of moloney sarcoma virus. | the simian virus 40 (sv40) 72-base pair (bp) tandem-repeated sequences have a crucial role as an activator element in viral gene expression. we replaced the sv40 72-bp repeat with a 72-bp repeat derived from the long terminal repeat (ltr) of cloned moloney murine sarcoma virus (msv) dna. although there is no detectable sequence homology to sv40, the msv repeats can substitute functionally for the sv40 repeats and generate a viable virus in monkey kidney cells. | 1982 | 6276775 |
| pericellular matrix and cell surface glycoproteins of virus-transformed mouse epithelial cells. | mouse embryo mus musculus castaneous epithelial cells, transformed with moloney murine sarcoma virus (msv) or with ecotropic murine leukemia virus (mulv), were analyzed for production of pericellular matrix glycoproteins. the nontransformed, msv-transformed, and mulv-transformed cells produced fibronectin, laminin, type i collagen, and small amounts of type iii collagen when studied by immunofluorescence using specific antibodies. the virus-transformed epithelial cells produced enhanced amounts ... | 1982 | 6277476 |
| expression of transforming region of moloney murine sarcoma virus in escherichia coli as a fusion protein with small tumor antigen of polyoma virus. | bacterial expression of the transforming region of moloney murine sarcoma virus, designated mos, was obtained as a fusion protein with a portion of the small tumor antigen of polyoma virus. this was accomplished by fusing the entire mos open reading frame, encoding a 41,000-dalton protein, with a plasmid that expresses a beta-galactosidase-polyoma fusion protein under lac operon control. the resulting plasmid directed synthesis of the predicted polyoma antigen-sarcoma virus fusion protein of 59, ... | 1982 | 6278495 |
| molecularly cloned c-mos(rat) is biologically active. | a unique rat cellular gene, c-mos(rat), homologous to the transforming sequences, v-mos, of moloney murine sarcoma virus (m-msv) was detected by hybridization to a v-mos specific probe. the c-mos(rat) gene was cloned together with its flanking sequences in an 11-kbp ecori dna fragment inserted in vector charon 4a. two probes were used to investigate the position and orientation of c-mos(rat) in the clone examined ( d3e ), namely pmsv -31 which contains the sequences specific for the transforming ... | 1982 | 6327263 |
| granulopoiesis longevity in continuous bone marrow cultures and factor-dependent cell line generation: significant variation among 28 inbred mouse strains and outbred stocks. | longevity of granulopoiesis in corticosteroid-supplemented continuous bone marrow cultures and generation of permanent wehi-3 dialyzed conditioned medium (dcm)-dependent hematopoietic progenitor cell lines were evaluated for 28 inbred mouse strains and outbred stocks. cultures from akr/j, nzw, c58/j, dba/2j, and c57bl/ksj mice generated granulocytes for 45-58 weeks and released more than 10(5) murine sarcoma virus (musv) rescue units of endogenous retrovirus; nonadherent (na) cells from 50% or m ... | 1982 | 6950163 |
| transforming growth factor(s) production enables cells to grow in the absence of serum: an autocrine system. | kirsten murine sarcoma virus (kimsv)-transformed rat-1, normal rat kidney (nrk), and balb/c 3t3 cells are capable of continual growth in a serum-free medium supplemented with transferrin and insulin but with no exogenous mitogenic growth factors. cells transformed by a mutant of kimsv that is temperature sensitive for the maintenance of transformation grow in this medium at the permissive temperature only. at the nonpermissive temperature, growth is dependent upon the presence of serum-free cond ... | 1982 | 6952201 |
| binding of retrovirus-associated protein kinase and proteins to staphylococcus aureus. | formalin-fixed staphylococcus aureus strain cowan, bearing protein a, routinely used for the absorption of antigen-antibody complexes, was found to bind protein kinase activity from disrupted moloney murine leukaemia virus (mo-mulv). the wood strain of s. aureus lacking protein a also bound the kinase with similar efficiency. about 50% of the bound kinase activity, as detected by phosphorylation of casein using [gamma-32p]atp, could be eluted from the bacterial preparation with buffer containing ... | 1982 | 6955449 |
| regulation of cell-mediated immunologic reactivity to moloney murine sarcoma virus-induced tumors. iii. further characterization of tumor-specific serum blocking factors. | blocking factors in the sera of inbred cba mice bearing either moloney murine sarcoma virus-induced tumors or transplanted 3-methylcholanthrene-induced tumors specifically blocked in the leukocyte adherence inhibition reaction between tumor-sensitized peritoneal cells and tumor antigens. these factors were absorbed from serum by treatment with anti-ia antibodies and could be eluted in a partially purified form. chromatography on sephacryl s-200 columns demonstrated blocking activity in fractions ... | 1982 | 6750199 |
| specific immunotherapy combined with whole body irradiation of balb/c mice bearing moloney murine sarcoma virus-induced primary tumors. | adoptive transfer of lymphocytes was performed into mice bearing progressor moloney murine sarcoma virus (m-msv)-induced primary tumors. lymphocytes from mice immunized against m-msv (designated as immune lymphocytes, il) inhibited tumor growth when transferred before the virus injection. however, the same lymphocytes were ineffective against tumors consisting of a solid mass when transferred after the virus inoculation. when il was transferred shortly after 500 rad whole-body irradiation of the ... | 1982 | 6751920 |
| mouse cells contain two distinct ras gene mrna species that can be translated into a p21 onc protein. | the kirsten (ki) and harvey (ha) strains of murine sarcoma virus encode a 21,000-dalton protein (p21 ras) which is the product of the transforming gene of these viruses. normal cells express low levels of p21 ras encoded by cellular genes (ki-ras and ha-ras) homologous to the ki and ha murine sarcoma virus transformation genes. a bone marrow-derived mouse cell line, 416b, has been shown to express unusually high levels of p21 ras. in this manuscript, we investigated the molecular biology of p21 ... | 1982 | 6131379 |
| the possible role of cellular genes related to retroviruses in the process of chemical and radiation carcinogenesis. | chemical carcinogens, radiation, and tumor promoters can not introduce new genetic information into cells. they must, therefore, call upon genes already present in the target cell. we have recently explored the possibility that these host genes share homology with specific nucleic acid sequences present in some of the known retroviruses. in one set of experiments we used a cloned dna probe prepared from the oncogene (v-mos) of moloney murine sarcoma virus (mo-msv) to examine the state of integra ... | 1982 | 6897728 |
| demonstration of biological activity and nucleotide sequence of an in vitro synthesized clone of the moloney murine sarcoma virus mos gene. | a clone of the moloney murine sarcoma virus mos gene derived by in vitro reverse transcription was characterized. when assayed for focus formation by dna transfection on nih/3t3 cells, this clone was biologically inactive, presumably due to the absence of a long terminal repeat sequence. therefore, a long terminal repeat was inserted into the clone by in vitro recombination, after which the most gene was able to transform nih/3t3 cells efficiently. the nucleotide sequence encompassing the transf ... | 1982 | 7045395 |
| tumor sponge implantation: an in vivo method for studying syngeneic, primary antitumor lymphocyte responses. | sponge matrices surgically implanted in the s.c. space of the back of normal balb/c mice were injected with a "regressor" dose of moloney virus-induced balb/c tumor cells. the kinetics of the generation of cytotoxic cells within the sponge was studied over a 22-day period in a short-term 51cr release assay. cytotoxic activity peaked on day 16 and then declined to negligible levels by day 22. no cytotoxicity was detectable when nontransformed balb/c blast cells, moloney leukemia virus-induced tum ... | 1982 | 7055792 |
| potential identification of chemical carcinogens in a viral transformation system. | chemical carcinogens from several diverse chemical classes i.e.; aromatic amines, polycyclic hydrocarbons, nitrosamines, hormonal derivatives, metals and direct alkylating agents cause a 6.2-60.5-fold increase in the frequency of murine sarcoma virus (msv)-induced transformation in a normal rat kidney (nrk) cell system. exogenous metabolic activation with a rat liver s-9 homogenate is required for expression of this activity by procarcinogens. non-carcinogenic analogs of these compounds fail to ... | 1982 | 7055852 |
| isolation of a transformation-defective deletion mutant of moloney murine sarcoma virus. | a transformation-defective (td) deletion mutant of moloney murine sarcoma virus (td mo-msv) and a transforming component termed mo-msv 3 were cloned from a stock of clone 3 mo-msv. to define the defect of the transforming function, the rna of td mo-msv was compared with those of mo-msv 3 and of another transforming variant termed mo-msv 124 and with helper moloney murine leukemia virus (mo-mulv). the rna monomers of td mo-msv and mo-msv 3 comigrated on polyacrylamide gels and were estimated to b ... | 1982 | 7077752 |
| possible role of a retrovirus in the expression of tumor-specific antigens of the meth a sarcoma. | the serologically defined tumor-specific surface antigen (tssa) of the chemically-induced balb/c meth a sarcoma, highly restricted to one of 20 sarcomas of balb/c origin, has been detected on a moloney murine sarcoma virus (mo-musv)-transformed balb/c 3t3 cell lines, designated iia(v). the immunogenicity of the iia(v) cell in tumor-rejection assays was specific for the meth a sarcoma, supporting the evidence for a close relationship between the tssa and the tumor-associated transplantation antig ... | 1982 | 7107069 |
| neoplastic transformation of rabbit cells by murine sarcoma viruses. | neoplastic transformation of rabbit cells by kirsten murine sarcoma virus (ki-msv), the ki-msv pseudotype of baboon endogenous virus (ki-msv[baev]) and the moloney-msv pseudotype of feline leukemia virus (m-msv[felv]) is reported. rabbit cells can be readily transformed by ki-msv, ki-msv(baev) and m-msv(felv). rabbit cells transformed by ki-msv and m-msv(felv) were found to be virus producers, whereas those transformed by ki-msv(baev) were nonproducers (np). the np cells were obtained by simply ... | 1982 | 7129681 |
| cells transformed by rna and dna tumor viruses produce transforming factors. | the rna tumor viruses, kirsten murine sarcoma virus (kimsv) and abelson murine leukemia virus, are shown to produce transforming factors (tfs) similar to sarcoma growth factor (sgf) produced by moloney murine sarcoma virus-transformed 3t3 cells. tf production by kimsv-transformed cells is dependent on the presence of a functional viral genome. tfs from kimsv-transformed cells induce the transformed phenotype in normal cells, which suggests that the tfs play a integral role in transformation. we ... | 1982 | 7141000 |
| specific chromosome changes associated with viral transformation of rat glial cells. | karyotypes of three malignant cell lines derived from wistar and wka/mk fetal rat glioblasts, transformed by murine sarcoma virus (msv-m-os) as well as those of four cell lines derived from c6 glioma cells of wistar origin, retransformed by msv-m-os, were analyzed in early culture passages. the c6 line had a modal number of 42 chromosomes with a normal male karyotype, and only a minor population of cells with 43 chromosomes. the modal chromosome number in every transformed glial cell line shifte ... | 1982 | 7141743 |
| persistence of one species of oncovirus in mixed infection after antiserum treatment or transspecies rescue. | attempts were made to change the envelope of murine sarcoma virus (msv) from b-mux (a xenotropic murine leukaemia virus isolate) to feline leukaemia virus (felv) by infecting cat cells with msv(b-mux), adding excess felv as helper and using mouse serum oncovirus-inactivating factor or anti-b-mux serum. in several attempts, virus from single foci of msv initially contained only msv(felv) but on passage each showed a minimal persistence of b-mux. to eliminate the b-mux component, msv(b-mux) was su ... | 1982 | 7142977 |
| resistance to moloney murine sarcoma virus-induced tumorigenesis in nk-deficient beige mice. | 1982 | 7159936 | |
| different sensitivities to external atp in passive permeability of transformed mouse cell lines. | external atp causes a passive permeability change in several transformed cells but not in untransformed mouse fibroblasts. in the present experiments, the atp-specific permeability change was observed also in b16 melanoma and l929 cells. the sensitivity to external atp of these cells increased further when the concentration of intracellular atp was lowered by addition of a mitochondrial inhibitor. in contrast, mouse 3t3 cells transformed by murine sarcoma virus did not respond to external atp ev ... | 1982 | 7161938 |
| characterization of a large genomic size moloney murine sarcoma virus produced by a transformed rat cell line. | a rat cell line (78a1) transformed by the moloney murine sarcoma virus-moloney murine leukaemia virus (mo-musv-mulv) complex was found to produce a sarcoma virus different from the isolates previously described. analysis of intracellular rna of the 78a1 cell line by electrophoresis on agarose gel, and hybridization with dna probes specific to m1-mo-musv and v-mosmo sequences revealed a size of 6.7 kilobases (kb) for the rna of this sarcoma virus. this genome is larger than those of m1, m3, ht1 a ... | 1982 | 7175503 |
| localization of the normal allele of t24 human bladder carcinoma oncogene to chromosome 11. | the development of dna-mediated gene transfer techniques has made it possible to identify transforming genes present in certain human tumour cells. such genes have been shown to induce morphological transformation when used to transfect suitable assay cells. recently a transforming gene has been isolated by molecular cloning techniques from the t24 (ref. 11) and ej (ref. 12) human bladder carcinoma cell lines. this bladder carcinoma oncogene has been shown to be of human origin, less than six ki ... | 1982 | 7177197 |
| oncogenic genes and human malignancy. | all vertebrates possess a series of genes which are homologs of the oncogenic genes of acute transforming retroviruses. two lines of evidence suggest that these genes may play a role in the development of human malignancy: (1) dna from a variety of human tumors transforms nih 3t3 mouse fibroblasts and the transforming genes from a number of carcinomas, sarcomas, and hematological malignancies have been identified as members of a family of genes, the ras family, closely related to the oncogenic g ... | 1983 | 6636835 |
| transformation of bloom's syndrome fibroblasts by dna transfection. | nonmalignant diploid human fibroblast cells (gm3498b) derived from a skin biopsy of a patient with bloom's syndrome have been transformed by transfection with dna from a tumorigenic mouse cell line (ha-8) carrying a single copy of the harvey murine sarcoma virus (ha-musv) genome. the transformed cell lines have an extended life-span, form colonies in agarose, and proliferate in nude mice--characteristics of neoplastic transformation. like the parental cells, they also exhibit a high spontaneous ... | 1983 | 6648529 |
| cell surface glycolipids of transformed nih 3t3 cells transfected with dnas of human bladder and lung carcinomas. | neutral glycolipids and gangliosides of nih 3t3 cells oncogenically transformed by transfection of dnas from human lung carcinoma (lx-1) and human bladder carcinoma (ej) have been investigated. the chemical quantity and the degree of cell surface exposure of gangliotriaosylceramide (gg3) were greatly enhanced in nih 3t3 cells transformed by transfection of dnas of either lx-1 or ej carcinoma cells. an identical but more conspicuous change in cell surface exposure of gg3 was observed in balb/c 3t ... | 1983 | 6667678 |
| [characterization of a subgenomic viral rna in the polysomes of cells infected with the moloney murine sarcoma virus]. | we have detected a 1.6 kb rna species in the polysomes of cells infected with the m1 strain of moloney murine sarcoma virus. this rna contains the cellular transforming sequences (mos) as well as sequences located at both ends of the viral genome and is devoid of other internal viral sequences. this rna appears to be spliced and might function as the messenger for the transforming protein of the moloney murine sarcoma virus. | 1983 | 6414656 |
| increased sensitivity of cultured fibroblasts from colon cancer-prone rats to cytotoxicity of carcinogens and to viral transformation: a comparison with fibroblasts from patients with adenomatosis coli. | lethal effects of mitomycin c (mmc), 4-nitroquinoline 1-oxide (4nqo) and ultraviolet light (uv) on fibroblast cell lines derived from a colon cancer-prone substrain of wistar-furth rats (wf/osaka rats) were measured in terms of the cellular colony-forming ability, and compared with the sensitivity to these agents of human fibroblasts from patients with adenomatosis coli and rectum (acr). all 6 fibroblast strains from the cancer-prone wf/osaka rats were significantly more sensitive (though to var ... | 1983 | 6421645 |
| recombinational junctions of variants of moloney murine sarcoma virus: generation and divergence of a mammalian transforming gene. | different variants of moloney murine sarcoma virus (msv) were examined by nucleotide sequencing to compare the junctions between the acquired cellular sequence, v-mos, and the adjacent virus-derived sequences. these variants included 124-msv, m1-msv, and ht1-msv and also the purportedly independent isolate gazdar msv. these four strains have an identical 5' junction between the murine leukemia virus env gene and the v-mos gene. this junction lies within the sixth codon of the chimeric env-mos co ... | 1983 | 6300424 |
| mutant feline sarcoma proviruses containing the viral oncogene (v-fes) and either feline or murine control elements. | the sequences required for transformation by the gardner-arnstein (ga) strain of feline sarcoma virus (ga-fesv) were defined by site-directed, in vitro mutagenesis of molecularly cloned proviral dna. portions of the ga-fesv provirus, subcloned in the plasmid pbr322, were mutagenized by deletion or frameshift at xhoi restriction sites flanking the nucleotide sequences presumed to encode the ga-fesv transforming polyprotein (p108(gag-fes)). the biological activity of subgenomic and reconstructed f ... | 1983 | 6300443 |
| detection of an 85,000-dalton phosphoprotein in ts110 murine sarcoma virus-infected cells with antiserum against a v-mos peptide. | we have used an antiserum directed against a synthetic v-mos peptide (anti-c3 serum) to screen ts110 murine sarcoma virus (musv)-infected cells for the presence of v-mos-encoded proteins. anti-c3 serum specifically recognized an 85,000-dalton protein doublet (p85) from [35s]methionine-labeled ts110 musv-infected producer cells grown at 32 degrees c, the permissive temperature for transformation. the p85 doublet was also recognized by an antiserum directed against the viral gag protein p15. p85 w ... | 1983 | 6300455 |
| p85: a gag-mos polyprotein encoded by ts110 moloney murine sarcoma virus. | antibody to a synthetic peptide (anti-c3 serum) with the predicted sequence of the c terminus of the moloney murine sarcoma virus (strain 124) v-mos gene was used in immunoprecipitation experiments with cytoplasmic extracts of a clone of nrk cells infected with ts110 moloney murine sarcoma virus, termed 6m2 cells. ts110 moloney murine sarcoma virus codes for two viral proteins of 85,000 and 58,000 m(r), termed p85 and p58, respectively, in nonproducer 6m2 cells maintained at 33 degrees c. anti-c ... | 1983 | 6300456 |
| nucleotide sequence of the rasheed rat sarcoma virus oncogene: new mutations. | the nucleotide sequence of the oncogene of the rasheed strain of rat sarcoma virus was determined. the oncogene (ra-v-ras) encodes a 29,000-dalton (p29) transforming protein. this protein is distinct from the immunologically related 21,000-dalton protein (p21) of the harvey murine sarcoma virus in its amino terminus and in having additional mutations in its carboxyl terminus. although the functional significance of these changes is unknown, they appear to occur only in rat sarcoma virus. | 1983 | 6344220 |
| immunocytochemical localization in normal and transformed human cells in tissue culture using a monoclonal antibody to the src protein of the harvey strain of murine sarcoma virus. | using a rat monoclonal antibody directed against the p21 src protein of the harvey strain of murine sarcoma virus (msv), we have examined the reactivity of human cells in tissue culture using immunofluorescence and electron microscopy. qualitative results indicated that untransformed mouse and human fibroblastic cells have undetectable amounts of p21; these levels were greatly increased after transformation with harvey msv. a group of human tumor cell lines adapted to tissue culture were examine ... | 1983 | 6357812 |
| the transforming protein of moloney murine sarcoma virus is a soluble cytoplasmic protein. | the transforming gene, v-mos, of moloney murine sarcoma virus (m-musv) encodes a 37,000-dalton phosphoprotein, p37mos. since the biochemical function of this protein is unknown, we have determined the subcellular location of p37mos in m-musv 124-transformed cells. using two different methods of cell lysis and fractionation, we found that newly synthesized as well as mature p37mos is a soluble cytoplasmic protein. in agreement with these results, immunofluorescent staining of cells acutely infect ... | 1983 | 6380747 |
| analysis of the interaction between rauscher murine leukemia virus and murine cell membrane receptor by in vitro binding assay. | binding of 125i-labeled gp70 of rauscher murine leukemia virus (r-mulv) by 3 murine cell lines, balb/c-3t3, nih/3t3 and ka-31 (kirsten murine sarcoma virus transformed clone a-31 of balb/c-3t3) cells was measured. the binding was a saturable process, dependent on the concentration of gp70 and on the number of cells. in no experiment could we demonstrate any quantitative utilization of gp70 in the medium. however, gp70 remaining in the spent medium could be bound to fresh cells in a subsequent in ... | 1983 | 6825074 |
| rat mutant cells showing temperature sensitivity for transformation by wild-type moloney murine sarcoma virus. | to clarify the cellular target(s) of onc gene products of moloney murine sarcoma virus (mo-msv), we isolated seven mutant cells that exhibit temperature sensitivity for transformation by wild-type mo-msv from fischer rat cell line. five strains of these mutant cells showed normal virus production at the nonpermissive temperature when infected with mo-msv, suggesting that viral replication is not affected by these cellular mutations. four of these mutants were also temperature sensitive (ts) for ... | 1983 | 6829165 |
| proteoglycans from the substratum adhesion sites of msv-transformed balb/c 3t3 cells. | kirsten murine sarcoma virus-transformed balb/c 3t3 cells (kimsv) are highly tumorigenic and metastatic in the appropriate murine host, are loosely adherent to the tissue culture substratum, and can be readily detached from the substratum by ethylene glycol bis(beta-aminoethyl ether) n,n'-tetraacetic acid treatment leaving their adhesion sites as substratum-attached material. both long-term culture-generated adhesion sites (l-sam) of kimsv cells and newly formed adhesion sites of reattaching cel ... | 1983 | 6838200 |
| mouse skin carcinomas induced in vivo by chemical carcinogens have a transforming harvey-ras oncogene. | several groups have shown that the malignant phenotype can be transferred to nih/3t3 fibroblasts by incorporation of dna isolated from tumour cell lines. these studies have demonstrated that the transforming activity of dna isolated from human bladder, lung and colon carcinoma cell lines is related to an alteration of the cellular homologues of the ras genes of harvey or kirsten murine sarcoma viruses. it is, however, unclear what relevance these observations have to the multi-stage nature of tu ... | 1983 | 6843661 |
| a cancer-associated lactate dehydrogenase is expressed in normal retina. | an unusual isozyme of lactate dehydrogenase, lactate dehydrogenase k, is found in high concentrations in cultured cells transformed by the kirsten murine sarcoma virus and in many human cancer tissues. in experiments described here high levels of a lactate dehydrogenase k activity were detected in extracts of normal rodent retina. this activity had the same key properties as the human tumor isozyme, namely, a highly cathodic electrophoretic mobility and inhibition of enzymatic activity by oxygen ... | 1983 | 6857286 |
| [cellular factors participating in cell transformation]. | to clarify the cellular target(s) of onc gene products of moloney murine sarcoma virus (mo-msv), we isolated eight mutant cells that exhibit temperature-sensitivity for transformation by wild type mo-msv from f2408 (fischer rat cell line). these mutant cells showed normal growth and normal mo-msv production at the nonpermissive temperature, suggesting that cellular and viral replication are not affected by these cellular mutations. these mutant cells are divided into 3 classes on the bases of te ... | 1983 | 6881989 |
| blocking of cell-mediated immunity to moloney murine sarcoma virus-transformed cells by lactate dehydrogenase virus-antibody complex. | the enhancement of moloney murine sarcoma virus (m-musv) tumor growth in balb/c mice previously infected with lactate dehydrogenase virus (ldv) was shown to depend on the timing of the inoculations. mice infected with ldv either 3 or 4 days prior to the m-musv inoculations demonstrated enhanced tumor growth, whereas mice infected simultaneously with ldv and m-musv or with ldv 30 days prior to m-musv challenge did not demonstrate m-musv tumor enhancement. circulating immune complexes (cic) were i ... | 1983 | 6572738 |
| three human transforming genes are related to the viral ras oncogenes. | three distinct transforming genes present in human tumor cell lines are all related to the viral oncogenes of harvey and kirsten murine sarcoma viruses, designated v-h-ras and v-k-ras, respectively. the transforming gene of a bladder carcinoma cell line has been shown to be a human homolog to v-h-ras [parada, l. f., tabin, c. j., shih, c. & weinberg, r. a. (1982) nature (london) 297, 474-478; santos, e., tronick, s. r., aaronson, s. a., pulciani, s. & barbacid, m. (1982) nature (london) 298, 343 ... | 1983 | 6572964 |
| stimulation of secondary anti-msv cytolytic t lymphocytes with mbl-2 reconstituted membranes. | membranes and solubilized, reconstituted membranes from moloney-infected tumors mbl-2 have been used to stimulate in vitro secondary cytolytic t lymphocytes (ctl) in c57bl/6 mice primed and moloney murine sarcoma virus. membranes are shown to stimulate the generation of moloney-specific and h-2 restricted ctl. stimulation with solubilized, reconstituted membranes required the presence of rat lymphocyte con a supernatant (containing interleukin 2) during the culture. reconstituted membranes made ... | 1983 | 6600194 |
| p85gag-mos encoded by ts110 moloney murine sarcoma virus has an associated protein kinase activity. | a protein identified as p85(gag-mos) was shown to be phosphorylated when immunoprecipitates from ts110 moloney murine sarcoma virus transformed nonproducer cells (clone 6m2) were incubated with [gamma-(32)p]atp. the in vitro-labeled 85,000-dalton phosphoprotein comigrated on nadodso(4)/polyacrylamide gels with authentic phosphorylated p85(gag-mos). immunoprecipitates obtained with antisera prepared against rauscher murine leukemia virus core protein p30 were active in the immune complex kinase a ... | 1983 | 6601272 |
| cytotoxic t lymphocyte recognition of transfected cells expressing a cloned retroviral gene. | the lysis of murine sarcoma virus-murine leukaemia virus (msv-mulv)-induced tumour cells by cytotoxic t lymphocytes (ctl) appears to require that an antigen specified by msv-mulv, or induced by the infection, be presented in association with class i major histocompatibility complex antigens. the viral proteins of the tumorigenic mulv seem to be a part of the antigens recognized by these dually restricted anti-mulv ctl, but the precise nature of the putative viral antigen(s) recognized by ctl is ... | 1983 | 6605480 |
| characterization of eukaryotic transcriptional control signals by assay of herpes simplex virus type 1 thymidine kinase. | we describe the characteristics of a general assay for eukaryote transcription-control sequences using the herpes simplex virus (hsv) thymidine kinase (tk) gene. after transfection of cultured cells with tk-containing recombinant plasmids, two assays were used to measure gene expression: short term or transient levels of tk mrna and tk enzyme activity, and the rate of biochemical transformation from a tk- to a tk+ phenotype in selective growth medium (hat). deletion of the endogenous tk promoter ... | 1983 | 6319553 |
| enhanced transformation by a simian virus 40 recombinant virus containing a harvey murine sarcoma virus long terminal repeat. | we have constructed a recombinant simian virus 40 (sv40) dna containing a copy of the harvey murine sarcoma virus long terminal repeat (ltr). this recombinant viral dna was converted into an infectious sv40 virus particle and subsequently infected into nih 3t3 cells (either uninfected or previously infected with moloney leukemia virus). we found that this hybrid virus, svltr1, transforms cells with 10 to 20 times the efficiency of sv40 wild type. southern blot analysis of these transformed cell ... | 1983 | 6302472 |
| frequent activation of c-kis as a transforming gene in fibrosarcomas induced by methylcholanthrene. | the dna's from two of four methylcholanthrene-induced mouse fibrosarcomas contained transforming genes that were identical in their pattern of restriction endonuclease resistance to inactivation of biologic activity. this transforming gene was identified as the activated homolog of the kirsten murine sarcoma virus onc gene, v-kis. the finding that a defined carcinogen reproducibly leads to activation of kis as a transforming gene should be of value in elucidating the role of oncogenes in the neo ... | 1983 | 6302839 |
| sequence relationships between kirsten retrovirus genomes and the genomes of other murine retroviruses. | rna sequence relationships between the genomes of the kirsten murine sarcoma virus (msv-k) complex, the kirsten murine leukemia virus (mulv-k) complex, the gross murine leukemia virus (mulv-g), and the moloney murine leukemia virus (mulv-m) were investigated. sedimentation analyses revealed the expected 30 and 34 s rna subunits in the msv-k complex and a previously undetected 30 s rna subunit accompanying the 34 s rna subunit in the mulv-k complex. nucleic acid hybridization data indicated that ... | 1983 | 6302979 |
| gag-mos polyproteins encoded by variants of the moloney strain of mouse sarcoma virus. | two revertants of ts110 moloney murine sarcoma virus (musv) with wild-type musv phenotype were examined for the presence of mos gene products, ts110 musv has a temperature-sensitive defect in a function required to maintain the transformed phenotype. the nonproducer 6m2 cell clone transformed by ts110 produces an 85,000-da gag-mos protein (p85gag-mos) and a 58,000-da gag protein (p58gag). a spontaneous revertant (clone 54-5a4) of the 6m2 cell clone produces a 100,000-da protein (p100) recognized ... | 1983 | 6302990 |
| interferon-mediated inhibition of production of gazdar murine sarcoma virus, a retrovirus lacking env proteins and containing an uncleaved gag precursor. | htg2 cells are murine sarcoma virus-transformed hamster cells. these cells continuously produce gazdar sarcoma virus particles which are devoid of viral envelope proteins and which contain the uncleaved gag precursor polyprotein, pr65, as their major protein constituent. human interferon-alpha elicited an antiviral response in these cells as shown by the inhibition of replication of vesicular stomatitis virus in interferon-treated cells. extracellular production of the retroviral particles by th ... | 1983 | 6302994 |
| stimulation of cell proliferation by vitamin a derivatives on murine sarcoma virus-transformed mouse cells in serum-free culture. | the effect of retinoids (rds) on cell proliferation was studied in serum-free culture condition, using non-transformed and transformed derivatives of balb 3t3. cell proliferation of an sv40-transformed line was inhibited significantly by rd treatment. however, proliferation of two cell lines that were transformed by a kirsten and moloney strain of murine sarcoma virus (msv) and produced growth factor into culture medium, was remarkably stimulated by rds. addition of serum masked both the inhibit ... | 1983 | 6303821 |
| cellular responsiveness to growth factors correlates with a cell's ability to express the transformed phenotype. | five random subclones of the rat fibroblast line f2408 vary in their frequency of transformation by the unrelated kirsten murine sarcoma virus and abelson murine leukemia virus. the same pattern of sensitivity is displayed when the cells are induced to anchorage-independent growth (transformed) by epidermal, platelet-derived, and sarcoma growth factors, or by whole serum. our results demonstrate that a growth factor's ability to render cells anchorage independent is not unique to transforming gr ... | 1983 | 6307528 |
| leukocyte adherence inhibition response to murine sarcoma virus-induced tumors. ii. requirement for t-cell subpopulations and macrophages. | murine sarcoma virus (msv)-immune t cells from c57bl/6 mice respond to intact rbl-5 tumor cells with the production of leukocyte adherence inhibition factor (laif), which mediates an adherence inhibition response of macrophages. laif is elaborated by isolated lyt-2+ cells incubated with rbl-5 cells, whereas lyt-1+ cells elaborate a substance that enhances macrophage adherence. spleen macrophages or peritoneal exudate macrophages from msv-immune mice when present at concentrations of 0.1% changed ... | 1983 | 6307531 |
| vascular neoplasms induced in rodent central nervous system by murine sarcoma viruses. | mice and rats were injected intracerebrally with harvey or kirsten murine sarcoma viruses at 2 or 10 days after birth. animals exhibited neurologic symptoms at 10 to 30 days postinjection. brains, livers, and spleens were examined grossly and by light and electron microscopy. mice injected at 2 days and rats injected at 10 days developed multiple brain neoplasms derived from endothelial cells. this conclusion was reinforced by immunofluorescence studies showing factor viii expression on transfor ... | 1983 | 6308345 |
| design of a retrovirus-derived vector for expression and transduction of exogenous genes in mammalian cells. | we have developed a transfection vector for animal cells that contains long terminal repeat (ltr) sequences to promote expression. plasmid p101/101, a derivative of plasmid pbr322 containing the complete moloney murine sarcoma virus genome, was cut with restriction enzymes and religated so that both the 5' and 3' ltrs were retained and all but about 700 base pairs of the intervening viral sequences were removed. to test this vector, the escherichia coli gene gpt was cloned into a unique psti sit ... | 1983 | 6308426 |
| structure of the ki-ras gene of the human lung carcinoma cell line calu-1. | the homologue of the viral kirsten ras (v-ki-ras) gene found in the human lung carcinoma cell line, calu-1, has an intron-exon structure similar to that of the human homologue of the viral harvey ras (v-ha-ras) gene. a second, potential fourth coding exon is present in the human ki-ras gene and similar sequences are found in the kirsten murine sarcoma virus. cysteine is encoded at the twelfth amino acid position, suggesting that the calu-1 ki-ras gene has undergone a mutational activation at the ... | 1983 | 6308465 |
| structure and organization of the human ki-ras proto-oncogene and a related processed pseudogene. | analysis of the organization and nucleotide sequence of two human loci related to the transforming gene of kirsten murine sarcoma virus establishes one as a functional gene and the other as a processed pseudogene. the two final coding exons of the functional gene seem to have arisen by duplication. differentially spliced mrnas incorporating one or other of the duplicated exons probably served as the intermediates by which the viral transforming gene and the pseudogene were generated. this sugges ... | 1983 | 6308466 |
| localization of c-ras oncogene family on human germ-line chromosomes. | the c-ras family is a set of c-onc genes that are highly conserved in vertebrates. the genes in this family are homologous to the transforming genes of harvey and kirsten murine sarcoma viruses (v-ha-ras and v-ki-ras, respectively). using an in situ molecular hybridization method, we detected three sites on the human pachytene chromosomes that exhibited significant hybridization to v-ki-ras and v-ha-ras probes. these were chromomere positions that corresponded to bands 11p14.1, 12p12.1, and 12q2 ... | 1983 | 6308650 |
| transforming genes of human hematopoietic tumors: frequent detection of ras-related oncogenes whose activation appears to be independent of tumor phenotype. | we surveyed 22 human hematopoietic tumors and tumor cell lines for sequences capable of transforming nih 3t3 cells by dna transfection. a primary human acute myelogenous leukemia, a chronic myelogenous leukemia cell line, and cell lines derived from three independent acute lymphocytic leukemias demonstrated oncogenes capable of conferring the transformed phenotype to nih 3t3 cells through serial cycles of transfection. one of three transforming genes associated with acute lymphocytic leukemia ce ... | 1983 | 6308661 |
| high-level expression in escherichia coli of enzymatically active harvey murine sarcoma virus p21ras protein. | the gene for the harvey murine sarcoma virus (ha-musv) p21ras protein was fused to the amino-terminal portion of the bacteriophage lambda cii gene on the expression vector pjl6. the fusion was such that transcription was controlled by the well-regulated phage lambda pl promoter, and translation initiated in the cii gene continued in frame into the ras gene sequences that code for p21. when the pl promoter was derepressed, the escherichia coli cells harboring the fusion plasmid synthesized 23,000 ... | 1983 | 6308763 |
| induction of rat brain tumor with xenotropic pseudotype murine sarcoma virus. | the oncogenicity of xenotropic pseudotype kirsten murine sarcoma virus (msv) was investigated in sprague-dawley rats. when fetal or newborn rats were inoculated intracerebrally with xenotropic pseudotype msv, brain tumors developed after about one month. tumors were induced both in the cerebrum and the cerebellum. histologically, the tumors were predominantly glioblastoma multiforme and hemangioendotheliomas. in cerebellar lesions, malignant transformation of vascular endothelial cells, polycyst ... | 1983 | 6308962 |
| localization of lipid-protein and protein-protein interactions within the murine retrovirus gag precursor by a novel peptide-mapping technique. | in htg2 hamster cells infected with the replication-defective gazdar murine sarcoma virus only immature virus particles are formed, with the uncleaved gag precursor pr65 as the only major protein in the virion. we have investigated the structure of these particles by using in situ cross-linking followed by chemical and enzymatic cleavages of pr65 to localize sites of lipid-protein and protein-protein interactions. lipid-protein cross-links were localized within a 10-kda fragment in the p15 regio ... | 1983 | 6309837 |