Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| perturbation of endocrine functions during persistent infection of mice with lymphocytic choriomeningitis virus. | 1986 | 3724660 | |
| the use of lymphocytic choriomeningitis virus reassortants to map viral genes causing virulence. | 1986 | 3724665 | |
| dissecting the molecular anatomy of persistent infection with lymphocytic choriomeningitis virus. | 1986 | 3724670 | |
| mechanisms of lymphocytic choriomeningitis virus-induced hemopoietic dysfunction. | results of this study showed that lymphocytic choriomeningitis virus infection causes a marked activation of natural killer (nk) cells not only in the spleen but also in the bone marrow. this activity reached its peak at about day 3 of infection and declined after days 6 to 7. enhanced nk cell activity was found to correlate with decreased receptivity for syngeneic stem cells in bone marrow and spleen, with the notable exception that decreased receptivity persisted longer in bone marrow. treatme ... | 1986 | 3735489 |
| nucleotide sequence of the glycoprotein gene and intergenic region of the lassa virus s genome rna. | two overlapping cdna clones corresponding to the 5' region of the lassa virus s genome rna were isolated and their nucleotide sequences determined. similar to pichinde and lymphocytic choriomeningitis viruses (lcmv), lassa virus has an ambisense s rna. the precursor to the viral glycoproteins (gpc) is encoded in viral rna sequence originating at position 56 and terminating at position 1529 from the 5' terminus of the s rna. a short, noncoding, intergenic region capable of forming a hairpin struc ... | 1986 | 3750844 |
| biology of cloned cytotoxic t lymphocytes specific for lymphocytic choriomeningitis virus. vi. migration and activity in vivo in acute and persistent infection. | cloned cytotoxic t lymphocytes (ctl) specific for lymphocytic choriomeningitis virus (lcmv) were adoptively transferred to syngeneic mice acutely or persistently (carrier mice) infected with lcmv. although infectious virus was cleared from the spleens during acute lcmv infection begun 24 hr earlier and the spleens remained clear of virus for the 4 days of testing, there was no concomitant reduction of viral titers in lymph nodes. in contrast, adoptive transfer of cloned ctl into animals with per ... | 1986 | 2416821 |
| induction of alloreactive cytotoxic t cells by acute virus infection of mice. | alloreactive cytotoxic t lymphocytes (ctl) distinct from virus-specific ctl and activated natural killer (nk) cells were generated during acute lymphocytic choriomeningitis virus (lcmv) infection of c57bl/6j mice. the alloreactive ctl shared similar antigenic markers (thy-1.2+, lyt-2.2+, and asialo gm1-) with the virus-specific ctl that appeared at the same time 7 days postinfection, but had different target specificities. these alloreactive ctl lysed allogeneic but not syngeneic or xenogeneic t ... | 1986 | 2418107 |
| analysis of endogenous and exogenous antigens in the nervous system using whole animal sections. | we describe a method which uses protein blots of whole animal sections to map the distribution of exogenous and endogenous antigens to specific nuclei and tracts within the nervous system (ns). we propose this method as a new approach to analysis of the expression of ns gene products during development and to charting the course and molecular pathogenesis of infectious and immune disorders of the ns. | 1986 | 2420825 |
| molecular and topographic analysis of the glycoproteins of lymphocytic choriomeningitis virus. | 1986 | 2425233 | |
| viral nucleoprotein can be demonstrated on the surface of lymphocytic choriomeningitis virus-infected cells. | 1986 | 2425234 | |
| genetic reassortants of lymphocytic choriomeningitis virus: unexpected disease and mechanism of pathogenesis. | reassortant viruses of different strains of lymphocytic choriomeningitis viruses cause lethal disease after inoculation into neonatal balb/c wehi mice, but, in contrast, parental strains or reciprocal reassortants do not cause lethal disease. the disease is characterized by inhibition of growth and death. the pathogenic mechanism is the induction of interferon combined with higher virus titers and subsequent liver necrosis. the generation of lethal reassortants from nonlethal parent viruses like ... | 1986 | 2426464 |
| proteins of lymphocytic choriomeningitis virus: antigenic topography of the viral glycoproteins. | topographical relationships among antigenic sites on the envelope glycoproteins of lymphocytic choriomeningitis virus (lcmv) were established using a panel of monoclonal antibodies (mab) directed against viral gp-1 and gp-2. purified mab were radioiodinated and used as probes in a solid phase competitive binding assay. epitopes on lcmv gp-1 were found to cluster in four antigenic sites. five neutralizing mab raised by immunization with the we strain of lcmv reacted with a single topographic site ... | 1986 | 2426862 |
| dissecting the molecular anatomy of the nervous system: analysis of rna and protein expression in whole body sections of laboratory animals. | nucleic acid hybridization and protein blotting procedures have allowed the specific detection of both rna and protein gene products in whole body sections of a selected host. these procedures permit efficient and reproducible screening of both endogenous and exogenous (viral) gene products, thus facilitating the study of normal differentiation, the localization of virus and the monitoring of viral diseases. although success with rna hybridization has thus far been limited to exogenous viral gen ... | 1986 | 2428441 |
| mapping arenavirus genes causing virulence. | 1987 | 2435461 | |
| state of viral genome and proteins during persistent lymphocytic choriomeningitis virus infection. | 1987 | 2435462 | |
| functional analysis of t lymphocyte subsets in antiviral host defense. | the role of different t cell subsets in antiviral host defense was investigated by treating thymectomized c57bl/6 and cba/j mice with monoclonal rat anti-lyt-2 or anti-l3/t4 igg 2b antibodies 14 and 10 days before infection. this treatment depleted the respective t cell subsets to undetectable levels in peripheral blood when assayed by immunofluorescence. in mice treated with anti-lyt-2, induction of cytotoxic t cells was reduced to less than 1 to 2% after intravenous infection with armstrong st ... | 1987 | 2435794 |
| mechanism of recovery from acute virus infection. iv. questionable role of mononuclear phagocytes in the clearance of lymphocytic choriomeningitis virus from spleens of mice. | after intravenous infection of mice with 10(3) infectious units (iu) the we strain lymphocytic choriomeningitis (lcm) virus multiplied in the spleens (as in all other major organs), reaching more than 10(8) iu/g of tissue on days 4 to 5. subsequently, the virus was quickly eliminated, being below detectability usually by day 10. during the time of virus clearance, the mononuclear phagocytes (mnp) of the spleen were activated as revealed by suppression of growth of listeria monocytogenes and incr ... | 1987 | 2435795 |
| monoclonal antibodies against viral determinants are not restricted to the k/d end of the major histocompatibility complex. | monoclonal antibodies against lymphocytic choriomeningitis virus (lcmv), a natural, high-replicating, noncytolytic pathogen in mice, were obtained from fusions between myeloma cells and lymphoid cells of mice of different h-2 haplotypes at various times (4-24 d) after infection. supernatants from growing hybridomas were tested in a ria, and approximately 15% of all supernatants were positive when tested for specificity on infected vs. uninfected cells of different haplotypes. upon retesting for ... | 1987 | 2439638 |
| perturbation of differentiated functions in vivo during persistent viral infection. iii. decreased growth hormone mrna. | lymphocytic choriomeningitis virus (lcmv) persistent infection that results from the inoculation of c3h/st newborn mice causes growth hormone (gh) deficiency and associated disease characterized by both reduced weight and serum glucose levels. molecular analysis of pituitary nucleic acids shows gh deficient mice have, on average, fivefold reduced levels of gh mrna although the histopathology of such gh producing cells is normal. northern blots indicate that the length of gh mrna is comparable in ... | 1987 | 3811236 |
| molecular characterization of the genomic s rna segment from lymphocytic choriomeningitis virus. | we have used cdna clones derived from the genomic s rna segment of lymphocytic choriomeningitis virus (lcmv), armstrong strain, as hybridization probes to monitor virus gene expression during acute infections. our results with strand-specific probes confirm the ambisense character of the lcmv s rna segment and document the presence of both genomic sense and genomic complementary sense rna species over the time course of infection. we have used nucleotide sequence information to predict primary a ... | 1987 | 3824905 |
| antiviral antibody-producing cells in parenchymatous organs during persistent virus infection. | in mice persistently infected with lymphocytic choriomeningitis virus (lcmv), the parenchymatous organs contain infiltrates of mononuclear cells, the sizes and numbers of which vary between strains and become more numerous and extensive when the animals grow older. histologically, these were found to possess a tissue-like structure, and by use of immunohistologic procedures they were shown to contain plasma cells secreting igm and igg. cells of kidneys, livers, brains, and spleens of lcmv carrie ... | 1987 | 3546579 |
| site-specific antibodies define a cleavage site conserved among arenavirus gp-c glycoproteins. | arenaviruses share a common strategy for glycoprotein synthesis and processing in which a mannose-rich precursor glycoprotein, termed gp-c in lymphocytic choriomeningitis virus (lcmv), is posttranslationally processed by oligosaccharide trimming and proteolytic cleavage to yield two structural glycoproteins, gp-1 and gp-2. mapping the orientation and proteolytic cleavage site(s) in such polyproteins has traditionally required direct protein sequencing of one or more of the cleaved products. this ... | 1987 | 3546729 |
| molecular anatomy of viral disease. the george cotzias memorial lecture 1986. | 1987 | 3547169 | |
| baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. | the requirements for high level expression of three foreign proteins using the polyhedrin gene promoter of autographa californica nuclear polyhedrosis virus (acnpv, baculoviridae) have been investigated. in spodoptera frugiperda cells infected with the appropriate recombinant baculoviruses, the synthesis of the two s rna coded genes of lymphocytic choriomeningitis virus (lcmv; i.e. the nucleoprotein, n, and glycoprotein precursor, gpc), or the haemagglutinin gene of influenza a virus, appears to ... | 1987 | 3553425 |
| depression of virus-specific cytotoxic t-cell responses during murine malaria. | mice with self-limiting p. yoelii or fatal p. berghei infections exhibited a markedly impaired ability to mount specific splenic cytotoxic t-lymphocyte responses to immunization with infectious ectromelia (ev), vaccinia (vac), or lymphocytic choriomeningitis viruses (lcmv). lymph node responsiveness, however, was not impaired. primary ctl responses were depressed in mice immunized 7 days after p. berghei infection, while in p. yoelii-infected mice, depressed responses were detected only during t ... | 1987 | 3554117 |
| lymphocytic choriomeningitis virus can persistently infect thyroid epithelial cells and perturb thyroid hormone production. | although viral infection has been suspected as the cause of some thyroid disorders, there has been limited data to support this contention seriously. now we report the first evidence that lymphocytic choriomeningitis virus can persist in the thyroid gland, particularly thyroid epithelial cells in which thyroglobulin (tg) the precursor of thyroid hormone, is synthesized. concomitant with the infection of these cells is a significant reduction in tg mrna and in the level of circulating thyroid hor ... | 1987 | 3598562 |
| susceptibility of inbred syrian golden hamsters (mesocricetus auratus) to lethal disease by lymphocytic choriomeningitis virus. | an acutely lethal lcmv disease model has been established in the syrian golden hamster (mesocricetus auratus) in which lethality and disease are dependent upon both the inbred hamster strain and the lcmv strain. young adult inbred, male and female, hamsters were tested for lethal-disease susceptibility by lymphocytic choriomeningitis virus (lcmv) strains, we or armstrong (arm). with we inocula, pd4 and mha inbred hamsters were highly susceptible to a wasting disease. lvg and lhc inbred hamsters ... | 1987 | 3601946 |
| lack of correlation between serum titres of interferon alpha, beta, natural killer cell activity and clinical susceptibility in mice infected with two isolates of lymphocytic choriomeningitis virus. | intracerebral infection of adult immunocompetent mice with most strains of lymphocytic choriomeningitis virus (lcmv) caused a systemic infection and led to severe meningoencephalitis and death due to the induced t cell immune response. the susceptibility of congenic mice to the two plaque variants docile and aggressive of lcmv strain ubc was shown to be mouse strain-dependent. to investigate the possible correlation between acid-stable interferon (ifn) and natural killer (nk) cell responses and ... | 1987 | 3612089 |
| meningeal macrophages reflect lymphocytic choriomeningitis virus pathogenic phenotypes. | intracerebral (i.c.) infection of adult mice with lymphocytic choriomeningitis (lcm) virus can result in acute lethal central nervous system (cns) disease which is the result of the host's thymus-derived lymphocyte (t cell) response against the virus. whether the specific effector function of the t cell is that of a cytotoxic cell (tc) or a delayed-type hypersensitivity cell (td) is still under debate. we assumed that if td cells were important in pathogenesis then accessory cells in the brain ( ... | 1987 | 3629192 |
| immune therapy of a persistent and disseminated viral infection. | the mechanism of viral clearance was studied by using the mouse model of chronic infection with lymphocytic choriomeningitis virus. distinct patterns of viral clearance and histopathology were observed in different organs after adoptive immune therapy of persistently infected (carrier) mice. clearance from the liver occurred within 30 days and was accompanied by extensive mononuclear cell infiltrates and necrosis of hepatocytes. infectious virus and viral antigen were eliminated concurrently. th ... | 1987 | 3682061 |
| immunosuppression-induced susceptibility of inbred hamsters (mesocricetus auratus) to lethal-disease by lymphocytic choriomeningitis virus infection. | the role of the immune response in the pathogenesis of lethal and non-lethal lymphocytic choriomeningitis virus (lcmv)-infections of young adult syrian golden hamsters (mesocricetus auratus) of different strains was examined using immunosuppressive treatment with cyclophosphamide or with whole-body gamma-irradiation. in all hamsters, the lcmv strains, we and armstrong (arm), caused systemic infections and induced comparable serum lcmv-antibody titers. however, lethal wasting-disease occurred whi ... | 1987 | 3689153 |
| characterization of virus-specific cytotoxic t cell clones from allogeneic bone marrow chimeras. | we established several h-2-restricted lymphocytic choriomeningitis virus (lcmv)-specific cytotoxic t cell clones from spleens of virus-primed c57bl/6 or c57bl/10 (h-2b) and b10.br (h-2k) mice and from allogeneic c57bl/10----b10.br and b10.br----c57bl/10 bone marrow chimeras. two t cell clones of h-2b origin and restricted to h-2b, 3 of h-2k origin and restricted to h-2k were compared with two clones each derived from the two types of chimeras. their surface phenotype was found to be lyt-2+, l3/t ... | 1987 | 3493906 |
| aberrant t cells in beige mutant mice. | cytotoxic t lymphocyte (ctl) morphology and function was examined in beige (bg/bg) mutant mice during infection with lymphocytic choriomeningitis virus (lcmv). virus-specific, class i-restricted ctl activity mediated by total spleen leukocytes isolated from bg/+ or +/+ mice on days 7 or 9 postinfection with lcmv was moderately higher than that mediated by spleen cells isolated from bg/bg mice. the ctl generated in bg/bg mice had aberrant morphology. lyt-2+ cells isolated from bg/+ or +/+ mice ha ... | 1987 | 3494057 |
| mechanism of recovery from acute virus infection: treatment of lymphocytic choriomeningitis virus-infected mice with monoclonal antibodies reveals that lyt-2+ t lymphocytes mediate clearance of virus and regulate the antiviral antibody response. | after intravenous infection of mice, lymphocytic choriomeningitis virus multiplied in spleens and livers, attaining highest concentrations on days 4 to 6. the subsequent clearance was as rapid, and 8 to 10 days after inoculation, infectivity was usually below detectability. during the effector phase of virus elimination, both cytotoxic t-cell (ctl) activity and the number of cells producing antiviral antibodies were high. monoclonal antibodies directed against t lymphocytes and t-lymphocyte subs ... | 1987 | 3494855 |
| anti-asialo gm1 eliminates both inflammatory process and cytotoxic t-cell function in the lymphocytic choriomeningitis adoptive transfer model. | the induction of severe inflammatory process and fatal neurological disease by transfer of lymphocytic choriomeningitis virus (lcmv)-immune t cells into cyclophosphamide (cy)-suppressed lcmv-infected mice is greatly inhibited by treatment of these recipients with antibody to the asialo gm1 ganglioside (anti-asgm1). examination of cytotoxic activity in lymphoid tissue of the cy-suppressed recipients at 72 hr after cell transfer revealed that anti-asgm1 treatment prevented the development of the c ... | 1987 | 3495344 |
| the acute inflammatory process in murine lymphocytic choriomeningitis is dependent on lyt-2+ immune t cells. | virus-immune spleen cells induce fatal immunopathology following adoptive transfer into adult c57b1/6j mice that have been infected with lymphocytic choriomeningitis virus (lcmv) and immunosuppressed with cyclophosphamide. this is accompanied by the development of potent cytotoxic t-lymphocyte (ctl) activity of donor origin in the recipient spleen. both the capacity to trigger the acute meningitis observed at 72 hr and to generate ctl effectors in lymphoid tissue are completely abrogated by the ... | 1987 | 3495350 |
| morphologic analysis of the interactions between lymphocytic choriomeningitis virus-specific cloned cytotoxic t cells and virus infected targets. | the interactions between lymphocytic choriomeningitis virus-specific cloned cytotoxic t lymphocytes (ctl) and virus infected targets have been examined by electron microscopy. ctls, which were readily differentiated from target cells by the presence of cytoplasmic granular inclusions, made intimate contact with infected cells. some ctls contacted infected cells via numerous interdigitating processes; others were observed thrusting finger-like protrusions deep into the target cell; some were seen ... | 1987 | 3496491 |
| different tc response profiles are associated with survival in the murine lymphocytic choriomeningitis virus infection. | the pathogenicity of lymphocytic choriomeningitis virus (lcmv) inoculated intracerebrally (i.c.) varies with virus strain and dose as well as with the mouse strain used as host. recently, results have indicated that susceptibility to lethal disease correlates directly the ability of the host to produce early and high virus-specific tc activity. however, in the present studies we demonstrate that even though this holds true in many mouse/virus combinations, it does not apply in others. thus, in c ... | 1987 | 3496657 |
| biobreeding/worcester (bb/wor) rats are deficient in the generation of functional cytotoxic t cells. | the biobreeding/worcester (bb/wor) rat provides a good model of spontaneous autoimmune diabetes. there are several sublines of the bb/wor rat. the diabetes prone (dp) sublines develop diabetes at a frequency of 50 to 80% from 60 to 120 days of age. the dp rats are lymphopenic, have a severe deficit in phenotypic ox 19+ ox 8+ cytotoxic t cells (tc), and lack rt 6.1 t cells. these rats have a relative increase in ox 19- ox 8+ natural killer (nk) cells and in nk activity as compared with the diabet ... | 1987 | 3497974 |
| chemotactic effect of human recombinant interleukin 2 on mouse activated large granular lymphocytes. | human recombinant interleukin 2 (hril-2) was demonstrated in vitro to be chemotactic for mouse large granular lymphocytes (lgl) activated in vivo by virus infection. peritoneal exudate cells harvested from virus-infected mice were used as a source of lgl. lgl collected from mouse hepatitis virus-infected mice at 3 days postinfection were a source for nk 1.1 positive natural killer (nk)/lgl. lgl collected from mice treated with antiserum to gangliotetraosylceramide and infected with lymphocytic c ... | 1987 | 3498769 |
| effective clearance of a persistent viral infection requires cooperation between virus-specific lyt2+ t cells and nonspecific bone marrow-derived cells. | the lifelong chronic lymphocytic choriomeningitis virus (lcmv) infection established in neonatally or congenitally infected mice can be eliminated by adoptive transfer of lymphoid cells from lcmv-immune mice. in this study, we have identified the effector cells mediating the clearance of persistent and disseminated lcmv infection. using mice that are recombinant in the h-2 region and by selective depletion of lymphocyte subpopulations, we show that viral clearance was mediated by lcmv-specific l ... | 1987 | 3500329 |
| allogeneic h-2 antigen expression is insufficient for tumor rejection. | murine a strain (kkddld) sarcoma i (sai) tumor cells have been transfected with a cloned h-2kb gene. the resulting clones (skb clones) stably express high levels of a molecule that is serologically and biochemically indistinguishable from the h-2kb antigen. skb clones are not susceptible to cytotoxic t lymphocyte-mediated lysis by h-2kb-specific bulk, cloned, or h-2kb-restricted lymphocytic choriomeningitis virus-specific effectors. survival times of a/j and b10.a mice challenged i.p. with the h ... | 1987 | 3500477 |
| expression of pgp-1 (or ly24) by subpopulations of mouse thymocytes and activated peripheral t lymphocytes. | the expression of pgp-1 (ly24) by subpopulations of thymocytes was investigated and a subpopulation of lyt-2-/l3t4-/j11d- thymocytes was identified which contained significant numbers (80%) of pgp-1+ cells. among freshly isolated lymph node t cells but not cortisone-resistant thymocytes, pgp-1 expression was heterogeneous. stimulation of t lymphocytes with either concanavalin a or the combination of phorbol myristate acetate plus calcium ionophore resulted in increased pgp-1 expression which was ... | 1987 | 3102251 |
| nature of the inflammatory process in the central nervous system of mice infected with lymphocytic choriomeningitis virus. | 1987 | 3107910 | |
| phenotypic analysis of the inflammatory exudate in murine lymphocytic choriomeningitis. | the massive inflammation of the cerebrospinal fluid (csf) which occurs in adult mice injected with lymphocytic choriomeningitis virus (lcmv) has been analyzed by flow microfluorometry (fmf). the great majority of the t cells detected by direct examination of freshly obtained csf were found to be lyt-2+, with an almost total absence of l3t4+ lymphocytes. the lyt-2/l3t4 ratio of lymphocytes in blood was within normal limits. predominance of the lyt-2+ subset was confirmed by culturing the csf cell ... | 1987 | 3108445 |
| molecular and genetic aspects of the pathogenesis of viral infections of the central nervous system. | viral pathogenesis can be defined in terms of a series of successive interactions between a virus and its target host. in order for a virus to injure a target organ such as the central nervous system (cns), it must first enter the host animal, replicate in some primary site near its place of entry, spread from this site to the cns and infect and injure specific populations of cells within the cns. at each of these steps, the virus must avoid or overcome a variety of immunological and nonimmunolo ... | 1987 | 3315238 |
| analysis of the genomic l rna segment from lymphocytic choriomeningitis virus. | the arenavirus genomic l rna segment represents approximately 70% of the viral genetic material but current understanding of the organization, regulation, and functioning of the viral l products remains limited. biological studies with reassortant viruses have implicated the l rna segment in the lethal infection of adult guinea pigs produced by lcmv-we but no further explanation of the pathogenic process is presently available. we have initiated a detailed molecular analysis of lcmv l products b ... | 1987 | 3318094 |
| the development of multiple expression vectors for high level synthesis of eukaryotic proteins: expression of lcmv-n and acnpv polyhedrin protein by a recombinant baculovirus. | a copy of the polyhedrin gene promoter of autographa californica nuclear polyhedrosis virus (acnpv), in association with the coding region of lymphocytic choriomeningitis virus n protein (lcmv-n) and the relevant polyhedrin transcription termination signals, has been cloned into the unique ecorv site of a plasmid representing an ecori derived fragment of the acnpv genome. the cloning site is upstream (but in the opposite orientation) of the natural acnpv polyhedrin gene. the derived pacvc2 trans ... | 1987 | 3334094 |
| possible mechanisms in the pathogenesis of virus-induced diabetes mellitus. | insulin-dependent diabetes mellitus results from destruction of pancreatic beta cells. viruses and autoimmunity have been implicated as possible causes of beta cell destruction in genetically predisposed individuals. the evidence for viruses comes largely from experiments in animals, but several studies in humans point to viruses as triggers in the pathogenesis of diabetes in some cases. in animal models, at least 4 different possible mechanisms for virus-induced diabetes have been proposed. the ... | 1987 | 2824113 |
| severe and transient pancytopenia associated with a chronic arenavirus infection. | a so-called 'docile' strain of lymphocytic choriomeningitis virus (lcmv) lacks the ability to cause the fatal central nervous system syndrome, commonly associated with most other strains of this virus, in c3heb/fej mice. hematological evaluation during a 5 week period revealed that every mouse experienced a pancytopenia which was the most severe around three weeks post-infection. the abnormal red blood cell (rbc) morphology seen in the peripheral blood along with the increased reticulocyte count ... | 1987 | 2835466 |
| the immune response of the mouse to lymphocytic choriomeningitis virus. v. high numbers of cytolytic t lymphocytes are generated in the spleen during acute infection. | in the spleens of c57bl/6j (b6) and cba/j (cba) mice undergoing acute infection with lymphocytic choriomeningitis (lcm) virus, lymphocytes with the ability to develop in vitro into lcm virus-specific cytolytic clones were enumerated by use of the limiting dilution method. at intervals after virus inoculation, defined numbers of cells were cultivated with virus-infected syngeneic stimulator cells and t cell growth factor in multiple wells of microculture plates. after 7 days, individual cell cult ... | 1987 | 2886344 |
| accumulation and chemotaxis of natural killer/large granular lymphocytes at sites of virus replication. | a model for monitoring the accumulation of natural killer cell/large granular lymphocytes (nk/lgl) at a site of virus replication was studied by using mice infected i.p. with either lymphocytic choriomeningitis virus (lcmv), murine cytomegalovirus (mcmv), mouse hepatitis virus (mhv), pichinde virus, or vaccinia virus. an i.p. but not i.v. infection resulted in a localized increase in nk/lgl cell number (a fourfold to greater than 20-fold increase) and augmentation (a 10- to 20-fold increase) of ... | 1987 | 3027167 |
| viruses perturb lymphocyte functions: selected principles characterizing virus-induced immunosuppression. | 1987 | 3036182 | |
| persistent virus infection associated with chemical manifestations of diabetes. ii. role of viral strain, environmental insult, and host genetics. | abnormal glucose metabolism (hyperglycemia and/or aberrant glucose tolerance test) occurred over the life-times of mice persistently infected with lymphocytic choriomeningitis virus (lcmv). persistent infection could be initiated in both newborn and adult mice. for newborns, inoculation with any of several strains of lcmv (armstrong, e350, pasteur, traub or we) caused continuous infection, but such infection of adults required a selected lymphotropic variant of lcmv armstrong (clone 13). through ... | 1987 | 2949622 |
| virus-lymphocyte interaction: t cells of the helper subset are infected with lymphocytic choriomeningitis virus during persistent infection in vivo. | the lifelong persistence of lymphocytic choriomeningitis virus (lcmv) in neonatally or congenitally infected mice is accompanied by a suppression of virus-specific t-cell responses. in this study, we identified the subset of t cells infected with lcmv during persistent infection in vivo. using specific monoclonal antibodies to separate the different lymphocyte cell populations and employing both an infectious center assay and immunofluorescence to detect the virus, we found that infection is con ... | 1987 | 2952807 |
| molecular analysis of the antigen receptor of virus-specific cytotoxic t cells and identification of a new v alpha family. | we have determined the primary structure of the alpha/beta-antigen receptor of a lymphocytic choriomeningitis virus (lcmv)-specific cytotoxic t cell clone (p14). the beta chain of the t cell receptor is composed of v beta 8.1, d beta and j beta 2.4 gene segments while the alpha chain uses a member of the v alpha 2 family and j alpha ta31. southern blot analysis of a panel of six other independent lcmv-specific cytotoxic t cell clones did not reveal any preferential usage of the v alpha 2 or v be ... | 1987 | 2961577 |
| virus-lymphocyte interactions. ii. expression of viral sequences during the course of persistent lymphocytic choriomeningitis virus infection and their localization to the l3t4 lymphocyte subset. | viruses that cause in vivo persistent infections need to selectively compromise the host's immunologic surveillance machinery in order to survive. to understand the molecular basis of how this is accomplished we have analyzed persistent virus infection by lymphocytic choriomeningitis in its normal host, the mouse. earlier we noted by infectious center analysis that five in 10(4) lymphocytes carried by persistently infected mice contained infectious materials throughout the course of infection. a ... | 1988 | 2963865 |
| virus-triggered immune suppression in mice caused by virus-specific cytotoxic t cells. | normal mice infected with 10(5) infectious doses of lymphocytic choriomeningitis virus (lcmv, we isolate) generated a reduced or no t cell-independent igm and/or t cell-dependent igg response to a subsequent vesicular stomatitis virus indiana (vsv-ind) injection; this transient immune suppression lasted for weeks to months. connatally infected lcmv-carrier mice or acutely infected t cell-deficient nude mice had normal anti-vsv igm and igg or igm responses respectively. lcmv-infected nude mice tr ... | 1988 | 2966846 |
| t4+ t helper cell function in vivo: differential requirement for induction of antiviral cytotoxic t-cell and antibody responses. | this study documents the differential requirements of t4+ t helper cells in the induction of virus-specific cytotoxic t-lymphocyte (ctl) and antibody responses during acute lymphocytic choriomeningitis virus infection. two monoclonal antibodies (gk1.5 and rl172.4) directed against the l3t4 (t4) molecule were used for depleting t helper cells from mice. depletion of t4+ cells caused a pronounced suppression of antiviral antibody response (20-fold decrease) but had minimal effect on virus-specific ... | 1988 | 2966865 |
| virus-lymphocyte interactions. iii. biologic parameters of a virus variant that fails to generate ctl and establishes persistent infection in immunocompetent hosts. | viruses that cause in vivo persistent infections avoid the host's immunologic surveillance machinery. a major component of that armamentarium is virus-specific mhc-restricted cytotoxic t lymphocyte (ctl) response of the host. studies with lymphocytic choriomeningitis virus (lcmv) have uncovered a parental virus (ctl+) that in immuno-competent adults induces ctl and terminates acute infection and a variant (ctl-) that fails to elicit ctl responses and establishes a persistent state (r. ahmed et a ... | 1988 | 2967012 |
| thymic selection of h-2-incompatible bone marrow cells in scid mice. differences in t help for induction of b cell igg responses versus cytotoxic t cells. | mice with congenital severe combined immunodeficiency disease (scid) failed to mount either a t cell-independent igm or t cell-dependent igg anti-vesicular stomatitis virus (vsv) indiana (ind) response. they did not generate cytotoxic t cells against lymphocytic choriomeningitis virus (lcmv) or vaccinia virus, but exhibited nk cell-like activities. when scid mice were given bone marrow from syngeneic balb/c (h-2d) nu/nu mice, all immune responses were expressed at control levels. if scid mice we ... | 1988 | 2971754 |
| virtue in viruses. | 1988 | 2972062 | |
| contributions of host and donor t cells to the inflammatory process in murine lymphocytic choriomeningitis. | the severe inflammation characteristic of the infection of adult mice with murine lymphocytic choriomeningitis virus (lcmv) is induced earlier in unsuppressed, virus-infected recipients by the adoptive transfer of class i mhc-compatible, cd4- cd8+ lcmv-immune spleen cell populations. the time to onset of fatal lcm may also be slightly diminished, though not to the extent that would be expected from the enhanced kinetics of the extravasation of cells into cerebrospinal fluid. the development of s ... | 1988 | 2972391 |
| recovery from acute virus infection. role of cytotoxic t lymphocytes in the elimination of lymphocytic choriomeningitis virus from spleens of mice. | 1988 | 3052209 | |
| immunopathological reactions in viral infections of the central nervous system. | viral infections of the central nervous system (cns), as of any other organ, evoke humoral and cellular immune responses which enable the host to eliminate the pathogen. however, effective responses may themselves produce tissue damage sometimes exceeding that caused by the virus itself. the relative contribution of the various immunopathological mechanisms in human viral encephalitides remains mostly ill defined. most of our understanding of the immunopathogenesis in viral cns infections comes ... | 1988 | 3058742 |
| anti-lcmv immune serum preparation and its testing by complement fixation test and immunoelectroosmophoresis. | 1988 | 2899967 | |
| viral infection of neurons can depress neurotransmitter mrna levels without histologic injury. | neonatal mice inoculated with lymphocytic choriomeningitis virus (lcmv) have non-lytic persistent neuronal infection and disturbed behavior. we now show that lcmv replicates in neurons containing the neurotransmitter somatostatin without morphologic evidence of injury and that persistent neuronal lcmv infection in mice is attended by a decrease in brain levels of somatostatin mrna. brain levels of mrna for another neurotransmitter peptide, cholecystokinin, are not decreased. these data are the f ... | 1988 | 2908027 |
| inhibition of hsv-1 and vaccinia virus replication by cephalosporin derivatives. | derivatives of beta-lactam antibiotics of the cephalosporin type at 0.02-1 mm concentrations interfered with in vitro replication of two dna-containing viruses, herpes simplex i and vaccinia, but showed no effects on two rna-viruses, lymphocytic choriomeningitis virus and vesicular stomatitis virus, or on cell viability. the exact structure of the active compounds remains unknown, but opening of the beta-lactam ring appears to be a prerequisite for their formation. whereas cephalosporin derivati ... | 1988 | 2852920 |
| [the coagglutination reaction with arbo- and arenaviruses]. | 1988 | 2470204 | |
| temporal analysis of transcription and replication during acute infection with lymphocytic choriomeningitis virus. | we have analyzed the accumulation of viral genomic and messenger rnas in tissue culture cells during the first 24 hr of acute infection with lymphocytic choriomeningitis virus (lcmv). this has allowed comparison of the relative amounts of the genomic l and s rnas (both genomic sense and genomic complementary sense) and of nucleoprotein (np) and glycoprotein precursor (gp-c) mrnas. using these techniques np mrna was detected simultaneously with genomic s rna, but the amount of np mrna accumulatin ... | 1988 | 3336942 |
| lymphocytic choriomeningitis virus. x. demonstration of nucleoprotein on the surface of infected cells. | of a total of 17 monoclonal antibodies (mab) directed against structural proteins of the lymphocytic choriomeningitis (lcm) virus, 3 were specific for the viral nucleoprotein (p63) and attached to the plasma membrane of infected cells, as disclosed by the indirect immunofluorescence procedure and complement-mediated cytolysis. we had previously demonstrated that a portion of the nucleoprotein (p63e) was part of the envelope of the intact virion (m. bruns, w. zeller, h. rohdewohld, and f. lehmann ... | 1988 | 3336945 |
| persistent viral infection of the thyroid gland: alteration of thyroid function in the absence of tissue injury. | the possible role of viruses as the cause of some thyroid disorders was evaluated in three strains of mice neonatally infected with lymphocytic choriomeningitis virus. we report the first definitive evidence that viruses can persist in the thyroid gland, particularly thyroid epithelial cells in which thyroglobulin is synthesized. concomitant with the infection of these cells was a significant reduction in the level of thyroglobulin mrna and circulating thyroid hormones. another virus that causes ... | 1988 | 3338413 |
| inhibition of diabetes in bb rats by virus infection. | bb rats serve as a model for human insulin-dependent diabetes mellitus (iddm), since without insulin treatment, most 60-140-d-old animals die within 1 to 2 wk of developing polyuria, polydypsia, hyperglycemia, and hypoinsulinemia. lymphoid cells accumulate in the islets of langerhans and beta cells undergo destruction. we report that inoculation of such bb rats with lymphocytic choriomeningitis virus (armstrong strain, clone 13) reduces over a prolonged period the incidence of iddm, normalizes t ... | 1988 | 3343348 |
| molecular analysis of viral rnas in mice persistently infected with lymphocytic choriomeningitis virus. | infection of newborn mice with lymphocytic choriomeningitis virus (lcmv) results in a lifelong persistent infection. persistently infected animals continuously produce low levels of infectious virus and accumulate large amounts of intracellular viral nucleic acid (p. j. southern, p. blount, and m. b. a. oldstone, nature [london] 312:555-558, 1984). we have used gel electrophoresis and hybridization techniques to analyze viral rnas that appear during the establishment and maintenance of a persist ... | 1988 | 3346946 |
| organ-specific selection of viral variants during chronic infection. | this study demonstrates organ specific selection of viral variants during chronic lymphocytic choriomeningitis virus (lcmv) infection in its natural host. isolates with different biological properties were present in the central nervous system (cns) and lymphoid tissues of carrier mice infected at birth with the wt armstrong strain of lcmv. viral isolates from the cns were similar to the wt armstrong strain and induced potent virus-specific cytotoxic t lymphocyte (ctl) responses in adult mice an ... | 1988 | 3367096 |
| tumor necrosis factor alpha in cerebrospinal fluid during bacterial, but not viral, meningitis. evaluation in murine model infections and in patients. | to evaluate the potential role of cachectin/tnf-alpha in the pathogenesis of bacterial and viral meningitis, concentrations and kinetics of tnf-alpha were determined in cerebrospinal fluid (csf). after intracerebral, but not systemic, infection with listeria monocytogenes in mice, tnf-alpha was detected as early as 3 h after infection reaching maximum titers after 24 h. however, tnf-alpha was not found in serum during the course of listeria infection. in contrast to bacterial meningitis, no tnf- ... | 1988 | 3367098 |
| effects of persistent lymphocytic choriomeningitis virus infection on cholinergic neurons in the mouse. | we have examined the effects of persistent infection with the armstrong e350 strain of lymphocytic choriomeningitis virus (lcmv) on choline acetyltransferase activity in several regions of balb/c mouse brain. despite the presence of high titres of virus in brain for as long as 6 months, and a widespread distribution of virus antigen, no decreases in choline acetyltransferase activity could be demonstrated. the enzyme activity was increased in some regions of brain, showing an effect of the persi ... | 1988 | 3374738 |
| virus-induced immunosuppression: a murine model of susceptibility to opportunistic infection. | 1988 | 3392416 | |
| deleted viral rnas and lymphocytic choriomeningitis virus persistence in vitro. | lymphocytic choriomeningitis virus (lcmv) infection of most tissue culture cell lines results in a non-cytopathic persistent infection. persistent infections in vitro share many characteristics with persistent lcmv infection of mice; both are associated with decreased titres of infectious virus, restricted accumulation of viral glycoproteins at the surface of infected cells and the generation of interfering particles. we have used gel electrophoresis and hybridization techniques to analyse lcmv ... | 1988 | 3404118 |
| susceptibility and resistance of inbred strains of syrian golden hamsters (mesocricetus auratus) to wasting disease caused by lymphocytic choriomeningitis virus: pathogenesis of lethal and non-lethal infections. | in different strains of inbred syrian golden hamsters (mesocricetus auratus), the lymphocytic choriomeningitis virus (lcmv) strains we and armstrong (arm) produced systemic infection with infective virus and viral antigens detected predominantly in reticuloendothelial organs. host and virus strain-dependent fatal wasting disease also occurred. after infection with we, all mha and pd4 hamsters died of a progressive wasting disease and infectivity persisted in organs at relatively high titres. lsh ... | 1988 | 3411298 |
| host cell-dependent homologous interference in lymphocytic choriomeningitis virus infection. | the generation of virus progeny as well as transcription, translation, and replication of the viral small rna (s-rna), which codes for the nucleoprotein (np) and the glycoprotein precursor (gpc), was followed in l and mdck cells after infection with multiplicities (m.o.i.) ranging from 0.01 to 100. in l cells, the yields of both plaque-forming units and interfering particles varied inversely with the m.o.i. northern blot analysis revealed that early after infection with high multiplicity np-mrna ... | 1988 | 3413982 |
| weight loss in obese mice persistently infected with lymphocytic choriomeningitis virus is not associated with elevated tumor necrosis factor/cachectin activity in peritoneal macrophages. | c57bl/6 ob/ob (c57 ob/ob) mice infected persistently with lymphocytic choriomeningitis virus (lcmv) show cachexia as judged by a weight loss of greater than 20%. virus persists in a subset of macrophages. because a cachexic state occurs in several chronic debilitating diseases of humans, often accompanied by persistent microbial infections with macrophage/monocytic involvement and tumor necrosis factor (tnf) cachectin production, the role of tnf in the weightloss of ob/ob mice infected persisten ... | 1988 | 3414785 |
| production of b cell stimulatory factor-2 and interferon gamma in the central nervous system during viral meningitis and encephalitis. evaluation in a murine model infection and in patients. | synthesis of b cell-stimulating factor-2 (bsf-2) and ifn-gamma was shown in cerebrospinal fluids (csf) collected from mice with experimental viral meningitis. in the csf, the level of bsf-2 started to increase 24 h after intracerebral infection with lymphocytic choriomeningitis virus (lcmv) with rapid increase after day 4. ifn-gamma was not detected in the csf before day 5 or 6 after infection, but increased sharply thereafter. in athymic nude mice, lcmv infection did not result in meningitis, a ... | 1988 | 3135367 |
| increased bactericidal macrophage activity induced by immunological stimuli is dependent on interferon (ifn)-gamma. interference of anti-ifn-gamma but not anti-ifn-alpha/beta with modulation of macrophage activity caused by lymphocytic choriomeningitis virus infection or systemic graft-vs.-host reactions. | during generalized immune responses such as the acute phase of graft-vs.-host reaction (gvhr) or many systemic viral infections the macrophage-monocyte system of mice is activated as demonstrated by their increased bactericidal capacity against listeria monocytogenes. to study the effect of interferon (ifn)-gamma in maintainance of macrophage activity, lymphocytic choriomeningitis virus (lcmv) primed c57bl/6 mice or (c57bl/10 x b10.br) f1 mice undergoing a gvhr were treated with polyclonal speci ... | 1988 | 3138138 |
| the incidence of diabetes in bb rats is decreased following acute lcmv infection. | 1988 | 3250261 | |
| analyses of the cytotoxic t lymphocyte responses to glycoprotein and nucleoprotein components of lymphocytic choriomeningitis virus. | the outcome of infection by lymphocytic choriomeningitis virus (lcmv) in the natural murine host is determined in large part by the cytotoxic t lymphocyte response (ctl) mounted by the host. in order to define the specificities of ctl induced by lcmv infection, we have cloned and expressed the full-length nucleoprotein (np) gene and 75% of the glycoprotein (gp) gene of lcmv in vaccinia virus vectors and have used these recombinant viruses to sensitize syngeneic target cells to lysis by anti-lcmv ... | 1988 | 3257596 |
| immunogenetic analysis of cellular interactions governing the recruitment of t lymphocytes and monocytes in lymphocytic choriomeningitis virus-induced immunopathology. | the lyt2+ class i major histocompatibility complex (mhc)-restricted virus-immune t cells that induce murine lymphocytic choriomeningitis (lcm) are targeted onto radiation-resistant cells in the central nervous system of virus-infected mice. the use of appropriate bone marrow radiation chimeras as lcm virus-infected, (immunosuppressed recipients for immune t-cell transfer has established that, though bone marrow-derived cells can stimulate virus-specific cytotoxic t lymphocytes (ctl) in spleen, t ... | 1988 | 3258209 |
| t-cell tolerance: exposure to virus in utero does not cause a permanent deletion of specific t cells. | this study documents the curing of a congenitally acquired chronic viral infection and the acquisition of t-cell competence by a previously tolerant host. infection of mice with lymphocytic choriomeningitis virus (lcmv) is a classic model of viral persistence and antigen-specific t-cell unresponsiveness. mice infected at birth or in utero become lifelong carriers with no detectable virus-specific cytotoxic t lymphocyte (ctl) responses. this chronic infection can be eliminated by adoptive transfe ... | 1988 | 3258424 |
| clearance of lymphocytic choriomeningitis virus in antibody- and b-cell-deprived mice. | the role of antibody in immune recovery from infection with lymphocytic choriomeningitis virus (lcmv) strain we was evaluated in b-cell-depleted mice. mice were treated from birth with either affinity-purified rabbit anti-mouse immunoglobulin m (igm), normal rabbit immunoglobulin, or, alternatively, an affinity-purified monoclonal rat anti-mouse igm antibody (lo-mm-9); untreated mice served as controls. b-cell depletion was considered complete in specifically treated mice according to the follow ... | 1988 | 3258641 |
| virus-lymphocyte interactions. iv. molecular characterization of lcmv armstrong (ctl+) small genomic segment and that of its variant, clone 13 (ctl-). | immunocompetent adult mice mount a vigorous cytotoxic t lymphocyte (ctl) response against the armstrong (arm) 53b strain of lcmv after primary inoculation. in contrast, the clone 13 variant of arm 53b, originally isolated from the spleen of a persistently infected mouse, suppresses lcmv-specific ctl responses (r. ahmed et al. (1984) j. exp med 60, 521). the induction and generation of ctl maps to the short (s) rna segment and not the long (l) rna segment of lcmv (y. riviere et al. (1986) j. immu ... | 1988 | 3259346 |
| induction of t cell serine proteinase 1 (tsp-1)-specific mrna in mouse t lymphocytes. | an oligonucleotide probe corresponding to nucleotides of a cdna encoding the t cell-associated proteinase 1 (tsp-1) was chosen to study the induction and expression of tsp-1-specific transcripts in mouse t lymphocytes and tissues. we demonstrate that tsp-1 mrna is only expressed in activated t lymphocytes and is absent from all mouse tissues tested including those containing resting mature t lymphocytes. expression of the tsp-1 gene was observed in t lymphocytes in vitro in response to either ph ... | 1988 | 3260181 |
| mechanism of recovery from acute virus infection. vi. replication of lymphocytic choriomeningitis virus in and clearance from the foot of the mouse. | the hind foot was chosen for study of the mechanism by which adult mice clear lymphocytic choriomeningitis (lcm) virus. the t cell-mediated swelling that follows the local inoculation of virus allows parallel investigation of the infectious process and delayed-type hypersensitivity in one organ. a dose of 10(5) mouse infectious units (iu) was optimal, and in all mouse strains tested foot swelling commenced 6 days after injection, with maximal response on days 7 and 8. when mice were sensitized b ... | 1988 | 3261324 |
| genetic analysis of in vivo-selected viral variants causing chronic infection: importance of mutation in the l rna segment of lymphocytic choriomeningitis virus. | viral variants with different biological properties are present in the central nervous systems (cns) and lymphoid tissues of mice persistently infected with lymphocytic choriomeningitis virus (lcmv). viral isolates from the cns are similar to the original armstrong lcmv strain and induce potent virus-specific t-cell responses in adult mice, and the infection is rapidly cleared. in contrast, lcmv isolates derived from spleens of carrier mice cause persistent infections in adult mice. this chronic ... | 1988 | 3261347 |
| the complementary roles of cellular and humoral immunity in resistance to re-infection with lcm virus. | the mechanisms underlying resistance to re-infection with lymphocytic choriomeningitis virus (lcmv) were investigated. rechallenge with moderate doses of virus (10(3) ld50) did not lead to detectable re-infection nor to re-induction of virus-specific cytotoxicity. when higher doses of virus were used for rechallenge (10(6) - 10(8) ld50), significant re-infection as well as reactivation of cytotoxicity were observed. both resistance and memory expression were controlled by an antigen-specific, ra ... | 1988 | 3263315 |
| distinct patterns of virus-specific t cell-mediated cytolysis of transformed versus primary target cells. | ideas about the mechanism(s) by which cytotoxic t lymphocytes (ctl) lyse appropriate target cells are still controversial. we studied the action of established murine ctl clones as well as of freshly prepared primarily induced ctl against two types of target cells. transformed tumour cell lines (mc57g and l929) and untransformed cells such as peritoneal exudate cells (pec) or fibroblasts were examined as target cells by scanning and transmission electron microscopy and with the 51cr-release assa ... | 1988 | 3264082 |
| virus-specific memory t cells are pgp-1+ and can be selectively activated with phorbol ester and calcium ionophore. | memory lymphocytic choriomeningitis virus (lcmv)-immune cytotoxic t-lymphocyte precursors (ctlp) can be stimulated to proliferate and to mediate specific cytotoxic activity following incubation with phorbol myristate acetate (pma), calcium ionophore (cai), and interleukin 2 (il-2). this protocol can be used to selectively induced virus-specific ctl activity under both bulk culture and limiting dilution conditions, in the absence of added antigen. there is no concurrent stimulation of alloreactiv ... | 1988 | 3282677 |
| abrogation of diabetes in bb rats by acute virus infection. association of viral-lymphocyte interactions. | the bb rat spontaneously develops an insulin-dependent diabetes mellitus (iddm) that closely resembles this disease in man. the pathogenesis involves autoimmune destruction of pancreatic beta-cells. in the present study, inoculation of diabetes-prone bb rats at 30 days of age with a lymphotropic variant of lymphocytic choriomeningitis virus significantly reduced the incidence of diabetes. such virus-inoculated, diabetes-free rats had normal levels of pancreatic insulin and little or no mononucle ... | 1988 | 3283232 |
| molecular definition of a major cytotoxic t-lymphocyte epitope in the glycoprotein of lymphocytic choriomeningitis virus. | analyses with segmental reassortants of lymphocytic choriomeningitis virus (lcmv) rna have shown that cytotoxic t lymphocytes (ctl) are induced by and recognize proteins encoded by the viral short segment, which specifies two virus structural proteins, glycoprotein (gp) and nucleoprotein (np). expression of cdna copies of these genes in vaccinia virus vectors demonstrates that c57bl/6 (h2bb) mice mount significant ctl responses to both gp and np. we have used lcmv-specific h2bb-restricted ctl cl ... | 1988 | 2448497 |
| fine mapping of a peptide sequence containing an antigenic site conserved among arenaviruses. | the lymphocytic choriomeningitis virus (lcmv) structural glycoproteins gp-1 (mr 44k) and gp-2 (mr 35k) are encoded on a single intracellular proteolytic cleavage precursor glycoprotein, gp-c (mr 76k). we have used a series of synthetic peptides derived from the deduced amino acid sequence of lcmv gp-c to define an antigenic site containing two topographically overlapping epitopes. three mouse monoclonal antibodies directed against two epitopes on gp-2 were assayed for binding in solution phase b ... | 1988 | 2452517 |
| an acquired immune suppression in mice caused by infection with lymphocytic choriomeningitis virus. | a murine model of virally induced acquired immunodeficiency was analyzed in mice. the effect of systemic infection with various isolates of lymphocytic choriomeningitis virus (lcmv) on the capacity of mice to mount a t cell-independent igm and a t cell-dependent igg neutralizing antibody response against a subsequent infection with vesicular stomatitis virus (vsv) was analyzed. dba/2 mice infected with the lcmv-we isolate were impaired in their igm and igg responses to vsv. immune suppression wa ... | 1988 | 2452742 |