Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the effects of tumour necrosis factor alpha on mediator release from human lung. | although tumour necrosis factor alpha (tnf alpha) may be involved in the pathology of asthma, little is known about its role in mediator release from inflammatory cells in human lung. we investigated whether tnf alpha induced histamine release from mast cells in human chopped lung tissue and whether it modulated antigen-induced release of histamine and leukotrienes c4/d4/e4 from passively sensitized lung tissue. spontaneous histamine release in the presence of 1 nm tnf alpha for up to 4 h at 37 ... | 1995 | 8535096 |
| the maintenance of functional activity in human isolated bronchus after cryopreservation. | cryopreservation has been successfully used in the in vitro study of pharmacological responses of animal tissues and, to a limited extent, of human tissue. in this study, we examined the effect of cryopreservation on reactivity of human bronchus which was stored for a period of up to 3 weeks. thirty-two bronchial rings were obtained from each of four transplantation donors (four male, aged 32 +/- 15 years sd). eight rings from each patient were studied on day 0 (the day of retrieval) and an addi ... | 1995 | 8535098 |
| [comparison of intradermal and prick tests based on samples of the most common allergens in the zadar region]. | the practical clinical problem of evaluating the significance of positive dermal tests is present in daily in vivo diagnoses in allergology and clinical immunology. our research was aimed at a comparison of the two methods of dermal tests, namely, intradermal (i.d.) and prick tests, based on a sample of the most common allergenics in the zadar region. the intradermal method was applied to a group of 664 patients, the prick test was used in a group of 641 patients, and 60 patients were examined u ... | 1995 | 8538357 |
| distribution of dermatophagoides mite (acari: pyroglyphidae) antigens in homes of allergic patients in japan. | dust samples collected in 61 homes of patients with mite allergies and 11 homes of non-allergic people as controls were examined for antigen levels of dermatophagoides farinae hughes and dermatophagoides pteronyssinus (trouessart) to reveal the current distribution of allergenic mites in homes in japan. patient homes had higher antigen levels than control homes in comforters and pillows but not in futons, carpets, tatamis and wooden floors. samples with antigen levels of > or = 10 micrograms m-2 ... | 1995 | 8542786 |
| regulation and targeting of t-cell immune responses by ige and igg antibodies. | a set of chimeric antibodies with identical f(ab')2 fragments specific for the hapten 5-iodo-4-hydroxyl-3-nitrophenacetyl (nip), but with different human fc parts (gamma 1, gamma 2, gamma 3, gamma 4, epsilon), was used to compare the role of igg and ige antibodies in antigen presentation by human epstein-barr virus (ebv) b cells. two or three molecules of nip were coupled to one molecule of der pi (der pi-(3)nip), a major allergen of dermatophagoides pteronyssinus. both monomeric igg and perform ... | 1995 | 8550069 |
| [fauna and biology of the pyroglyphidae of poland]. | 5 valid species of pyroglyphid mites are actually known in polish fauna. it should be stressed that the knowledge of their occurrence in house-dust, bird nests and other habitats is still poor. the most common pyroglyphid house-dust mites, dermatophagoides pteronyssinus, d. farinae and euroglyphus maynei, were found in samples of dust from houses (warszawa, bydgoszcz, upper silesia), hospitals (katowice, sosnowiec), libraries (sosnowiec) and port buildings (gdynia). the both species of the genus ... | 1995 | 8553664 |
| human auto-anti-idiotypic antibodies to mite-specific ige can degranulate human basophils in vitro. | anti-idiotypic antibodies (anti-ids) to specific ige antibodies are formed spontaneously during an anti-allergen immune response and can be induced by immunotherapy. although anti-ids can down-regulate the production of ige antibodies, at least in experimental models, their possible role in the modulation of target cell reactivity remains ill-defined. | 1995 | 8556564 |
| relationship between facilitated allergen presentation and the presence of allergen-specific ige in serum of atopic patients. | allergen presentation to allergen-specific t cells can be facilitated when ige-allergen complexes are endocytosed by antigen-presenting cells (apc) after binding to the low-affinity fc epsilon r type ii (cd23). here we present a study on the relative capabilities of sera of atopic patients to mediate facilitated antigen presentation (fap). to this aim fap was studied in an in vitro model in which cd23-expressing epstein-barr virus (ebv)-b cells act as apc to t lymphocyte clones (tlc) that are sp ... | 1995 | 7851024 |
| the influence of increased bronchial responsiveness, atopy, and serum ige on decline in fev1. a longitudinal study in the elderly. | we have performed a 4-yr prospective survey of the association of allergen skin test positivity, total serum immunoglobulin e (ige), and bronchial responsiveness to methacholine, with longitudinal decline in fev1, in subjects over 65 yr of age. in 1987, 324 subjects completed a respiratory questionnaire, and underwent measurement of fev1 and fvc, methacholine challenge, skin prick testing (to dermatophagoides pteronyssinus, cat fur, mixed grasses, and aspergillus fumigatus) and estimation of tot ... | 1995 | 7881652 |
| [natural allergens and recombinant allergens]. | since forty years, many allergens from different species responsible for allergies, have been purified and sometimes identified using classical methods of protein chemistry. for the first time in 1988, molecular biology technologies were applied to allergens, namely to a major allergen of the mite, dermatophagoides pteronyssinus. during the recent years many other allergens have been produced as recombinant proteins expressed in bacteria or yeast leading to the growing family of recombinant alle ... | 1995 | 8590581 |
| multicentre evaluation of capture assay radim liquid allergen for measurement of specific ige antibodies. | a multicentre trial of capture assay radim liquid allergen was performed to define the sensitivity, specificity and clinical reliability of the system in diagnostic allergology. the results of the evaluation were compared with clinical data and in vivo testing. good agreement was obtained for dermatophagoides pteronyssinus (d1), cat's epithelium (e1), betula verrucosa (t3) and olea europea (t9), artemisia vulgaris (w6) and parietaria officinalis (w19). some spreading of data was observed for art ... | 1995 | 8608200 |
| ige-mediated basophil releasability is influenced by intrinsic factors and by ige on the cell surface. | experiments were done to clarify the mechanisms associated with releasability of histamine. first, washed leukocytes from 23 asthmatic patients sensitive to mite allergen were challenged with der p 1, a major allergen isolated from dermatophagoides pteronyssinus, or anti-ige. a significant correlation was observed between the ratio of der p 1-specific ige titer to total ige level (s/t) in the patient's plasma and either the reactivity (maximal percentage of histamine release; rs = 0.514, p = 0.0 ... | 1995 | 8834823 |
| lung cancer-related changing profile of b-cell epitopes recognized on mucosal antigens: the der p1 model. | the natural immunoglobulin g (igg) response towards antigens presented at the mucosal level in patients with lung cancer, the most frequent mucosal malignancy in humans was studied. compared with healthy control subjects, patients with lung cancer display lower igg antibody titers toward antigen p1 of the house dust mite, dermatophagoides pteronyssinus (der p1), chronically presented to the respiratory mucosa. the present study further characterizes this defect in terms of antibody specificity. | 1995 | 8630893 |
| comparative performance for immediate hypersensitivity skin testing using two skin prick test devices. | fifty-five patients were skin-tested by multi-test (m) and needle prick test (npt) to compare the reproducibility of the methods. we used 6 allergenic extracts: dermatophagoides pteronyssinus, dermatophagoides farinae, dog epithelium, cat pelt, american cockroach and mixed molds. a glycerosaline and a positive control (histamine 1 mg/ml) were performed in both methods. statistically significant differences in histamine and dermatophagoides farinae wheal reactions between the two methods (m > npt ... | 1995 | 8653226 |
| contact allergy to dermatophagoides in atopic dermatitis patients and healthy subjects. | to compare different house-dust-mite-derived allergenic materials and to correlate the presence of ige to dermatophagoides with patch test results, 313 atopic dermatitis (ad) patients and 100 healthy volunteers (hv) underwent patch tests with: dermatophagoides pteronyssinus (dpt) lyophilized purified alpha fraction in buffered saline/glycerol 50% and/or in petrolatum (bayropharm); 50% dpt and 50% dermatophagoides farinae (df) whole bodies in petrolatum and petrolatum oil (allergopharma-bracco); ... | 1995 | 8654075 |
| prevalence of sensitization to dermatophagoides pteronyssinus in several industrial populations. | skin tests with dermatophagoides pteronyssinus were carried out by the standard prick method in six groups of industrial workers: meat processing workers (n = 107), brewery workers (n = 96), animal food workers (n = 40), swine farmers (n = 32), paper-mill workers (n = 132) and wool-textile workers (n = 111). the control group consisted of 158 subjects who were tested during the preemployment examinations and had not worked in industrial plants before. skin reactions were read after 20 minutes by ... | 1995 | 8670010 |
| glutathione s-transferase a major allergen of the house dust mite, dermatophagoides pteronyssinus. | recently, we cloned a new dermatophagoides pteronyssinus allergen homologous with glutathione s-transferase. ige radioimmunoassays against the escherichia coli lysate of the recombinant clone reveal that 40% of mite allergic subjects recognize the mite glutathione s-transferase allergen. of these sera, greater that 50% have reactivity with the recombinant mite glutathione s-transferase that is greater than 10 times the result observed with a normal control. immunoblotting studies with sera from ... | 1995 | 8719107 |
| [risk indicators of reaginic allergy: relations between atopic dermatitis and serum specific ige of various pneumallergens]. | this study was conducted in 1,091 children and adolescents submitted to an allergy check-up for respiratory symptoms. all subjects had a research of ige for dermatophagoides pteronyssinus, cat and timothy grass pollen by cap-system pharmacia. three hundred subjects had a personal history of atopic dermatitis. they were compared to the 791 others. no difference was found for age, sex, family history of allergic respiratory disease, breast feeding nor exposure to tobacco smoke. the ige level was s ... | 1995 | 8734080 |
| the prevalence of atopic sensitization and respiratory symptoms among estonian schoolchildren. | to assess the prevalence of atopic sensitization, asthma and other respiratory disorders among estonian schoolchildren in a coastal industrial city and an inland university town, i.e. tallinn and tartu. | 1995 | 8821300 |
| month of birth, atopic disease, and sensitization to common aeroallergens in greece. | the aim of the study was to evaluate the influence of the month of birth on the development of atopic disease and the rate of sensitization to common environmental allergens in greece. analysis of the month of birth of 1755 individuals with atopic disease (asthma, rhinitis, atopic dermatitis), out of whom 771 had positive skin tests/rast to dermatophagoides pteronyssinus and mixed grasses, in comparison to total live births (2,877,733) in greece during 1968-88, was performed and showed significa ... | 1995 | 8822396 |
| environmental and bronchoalveolar lavage dermatophagoides pteronyssinus antigen levels in atopic asthmatics. | overnight environmental home exposure to dermatophagoides pteronyssinus in ige-sensitized individuals is often clinically associated with increased morning symptoms of rhinitis and/or asthma. we have investigated whether household exposure to d. pteronyssinus is associated with the presence of immunoreactive d. pteronyssinus i antigen (der p i) in bronchoalveolar lavage (bal) fluid in nine atopic asthmatics sensitized to d. pteronyssinus. significant environmental concentrations of d. pteronyssi ... | 1995 | 7812575 |
| eosinophilic pustular folliculitis in three atopic children with hypersensitivity to dermatophagoides pteronyssinus. | three children will be described who present recurrent episodes of pruritic papulopustular follicular lesions on the face, the extremities and the trunk. the episodes lasted for 1-3 months with intermittent remission. each flare was accompanied by hypereosinophilia and an increased total ige titer. rast and prick tests were positive for dermatophagoides pteronyssinus (dpt). laboratory tests disclosed no infectious or parasitic etiology. histological examination showed eosinophilic pustular folli ... | 1995 | 7727841 |
| [value of a specific bronchial provocation test in comparison with other methods in diagnosis of atopic bronchial asthma]. | the aim of this study was to determine the relation between specific bronchial provocation test (sbpt), the serum specific ige level (sige) and specific basophil histamine release (dpt hr) in asthma patients, sensitive to dermatophagoides pteronyssinus (dpt). the studies were carried out on 30 mild asthma patients with positive case history and strongly positive skin tests with dpt. in all the patients were performed following tests: bpt with histamine, serum specific ige level against dpt (sige ... | 1995 | 7544185 |
| vineyard snail allergy possibly induced by sensitization to house-dust mite (dermatophagoides pteronyssinus). | a female patient experienced a severe allergic reaction after consumption of vineyard snails. the patient proved to be sensitized to house-dust mite (hdm) and demonstrated a positive skin test and specific ige to snail (eobania vermiculata, lofarma). the snail rast was > 80% inhibited by hdm, whereas the mite rast was < 10% inhibited by snail extract. this is possibly another example of food allergy related to primary sensitization by an aeroallergen. | 1995 | 7573833 |
| effects of "systemic" budesonide concentrations on in vitro allergen-induced activation of blood mononuclear cells isolated from asthmatic patients. | blood levels of inhaled corticosteroids are significantly lower than those measured in the lung, but their concentration could still have anti-inflammatory effects. to determine whether budesonide, at concentrations similar to those obtained in blood after drug inhalation (10(-9) m), could downregulate the allergen-induced activation of mononuclear cells, we studied 21 atopic patients, sensitized to dermatophagoides pteronyssinus (der p). on blood mononuclear cells, isolated from these patients, ... | 1995 | 7573828 |
| eosinophil differentiation and hypodensity alteration activities in dermatophagoides pteronyssinus-stimulated mononuclear cell culture supernatants derived from asthmatics. | we investigated the contributing effect of dermatophagoides pteronyssinus-stimulated mononuclear cell (mnc) cultured supernatants on the differentiation and density alteration of eosinophils. mnc, obtained from either normal subjects or asthmatics, were cultured with or without d. pteronyssinus. the supernatants were tested for the activity of eosinophil differentiation and density alteration. the results showed a significant increase in eosinophil differentiation activity in d. pteronyssinus-st ... | 1995 | 7888793 |
| relationship between nasal resistance and airway hyperreactivity following nasal provocation with dermatophagoides pteronyssinus in allergic rhinitis. | to determine whether nasal allergic symptoms can cause bronchial hyperresponsiveness to methacholine, 30 subjects with allergic rhinitis (22 with allergic rhinitis and 8 with allergic asthmatic rhinitis) were studied. all subjects were skin test positive to dermatophagoides pteronyssinus (dp) and underwent nasal allergic provocation with dp. after provocation, there was a severe nasal allergic reaction in the challenged nostril with a significant increase in nasal resistance both immediately and ... | 1995 | 7888791 |
| hla-dr expression is induced on keratinocytes in delayed hypersensitivity but not in allergen induced late-phase reactions. | in view of increasing evidence suggesting an active immunoregulatory role of the skin keratinocytes and the observation that the differentiation of allergen specific t lymphocytes is critical in the development of allergy, we evaluated epidermal expression of hla-dr antigen in skin reactions induced with an atopen (house dust mite) and with an non-atopic antigen (hemocyanin). two groups of patients with house dust mite (dermatophagoides pteronyssinus [der p]) allergy were compared, one group was ... | 1995 | 7788573 |
| specific down-regulation of anti-allergen ige and igg antibodies in humans associated with injections of allergen-specific antibody complexes. | several approaches have recently been put forward describing attempts to suppress the ige immune response towards allergens, which is thought to be the key event in allergic diseases. in a series of clinical trials we have shown that injections of complexes made up from allergen and specific antibodies are an effective treatment for allergic bronchial asthma and atopic dermatitis. in the work presented here we have examined the humoral immunity changes associated with the use of such complexes i ... | 1995 | 7553070 |
| antibody-dependent cellular cytotoxicity, ingestion and digestion in dermatophagoides pteronyssinus-sensitive asthmatic children. | dermatophagoides pteronyssinus (dp) is, for unknown reasons, the commonest cause of asthma attacks in children suffering from reaginic bronchial asthma. the underlying immune disorder is also unclear. the authors analyzed phagocytosis (ingestion), digestion and antibody-dependent cellular cytotoxicity (adcc) of peripheral blood leukocytes in 20 asthmatic children hypersensitive to dp, aged 2 to 14 years. the tests were performed while the children were entirely asymptomatic and under no therapy. ... | 1995 | 8630450 |
| the effect of cetirizine on sulfidoleukotriene production by blood leukocytes in children with allergic rhinitis. | twelve children with allergic rhinitis due to monosensitivity to dermatophagoides pteronyssinus (dp) took part in a placebo-controlled, double-blind, crossover study to evaluate the effect of cetirizine, a second-generation, nonsedating h1-blocker-type antihistamine, on sulfidoleukotriene releasability by blood leukocytes and to determine its correlation with clinical findings and nasal challenge scores. sulfidoleukotriene release by blood leukocytes was determined by the cellular allergen stimu ... | 1995 | 8834825 |
| [effectiveness of oral immunotherapy in bronchial asthma caused by dermatophagoides pternyssinus]. | we have evaluated the efficacy of oral hyposensitivization in 14 patients suffering from bronchial asthma due to allergy to dermatophagoides pteronyssinus. the allergen extract was taken in increasing doses and the maximum dose was maintained for the duration of the study (two years). oral treatment was effective in reducing the number and severity of asthma attacks (p < 0.001) and in improving the respiratory peak flow (p < 0.001). no side effects were observed and all the patients concluded th ... | 1995 | 8588080 |
| double-blind trial of house-dust mite immunotherapy in asthmatic children resident at high altitude. | twenty-three dermatophagoides pteronyssinus (dpt)-sensitive asthmatic children aged 7-14 years entered a double-blind, placebo-controlled trial of standardized immunotherapy (it) (alpare) while resident at high altitude. dpt sensitivity was evaluated by skin prick tests at different allergen concentrations at the enrollment and after 6 and 12 months of treatment. bronchial hyperreactivity was evaluated at the same time points, and on each occasion, histamine challenge and, the following day, dpt ... | 1995 | 8748726 |
| the use of domestic steam cleaning for the control of house dust mites. | control of dust mites using extremes of temperature is an alternative to the use of acaricides. in the past we have attempted control by freezing with liquid nitrogen. the present paper deals with the opposite extreme, the use of steam. | 1995 | 8581838 |
| house-dust mite content in mattresses in relation to residential characteristics and symptoms in atopic and nonatopic children living in northern norway. | the occurrence of house-dust mites (hdms) was investigated in the mattresses of 19 children previously found to be skin prick test (spt) positive to hdm and in 19 nonatopic children derived from an extensive survey of 424 schoolchildren, all living in northern norway. domestic mites were counted and identified microscopically. mite counts ranging from 10 to 1800 mites per gram mattress dust were found in 10 of the 19 hdm-sensitized children compared to none in the control group, corresponding to ... | 1995 | 8607559 |
| molecular cloning and immunological characterization of the house dust mite allergen der f 7. | the allergen der p 7 from dermatophagoides pteronyssinus has been defined by molecular cloning and shown to be an important specificity in 50% of mite-allergic patients. | 1995 | 8556554 |
| ige antibodies reactive with silverfish, cockroach and chironomid are frequently found in mite-positive allergic patients. | approximately 30% of the house dust mite allergic patients in the netherlands have ige antibodies reactive with silverfish, cockroach and/or chironomid. in allergic patients without ige antibodies against dermatophagoides pteronyssinus less than 5% have ige antibodies reactive with these insects. by means of rast inhibition studies it is shown that cross-reactivity exists between d. pteronyssinus and silverfish, cockroach or chironomid. this means that a positive rast for silverfish, cockroach, ... | 1995 | 7549504 |
| intranasal immunotherapy with dermatophagoides extract: in vivo and in vitro results of a double-blind placebo-controlled trial. | intranasal immunotherapy (it) has been proposed as a means to induce an effective immunity of the nasal mucosa in patients with allergic rhinitis, avoiding systemic side effects. in the present study 20 individuals with chronic allergic rhinitis, and skin prick test reactive to dermatophagoides pteronyssinus (dp) only, were randomized and subjected to a three months' double-blind placebo-controlled trial of intranasal it with dp extract. all patients received also sodium cromoglycate as pre-medi ... | 1995 | 8560161 |
| diagnostic value of the nasal provocation test with dermatophagoides pteronyssinus in childhood asthma. | we aimed to determine whether the nasal provocation test (npt) with allergen could be used as a diagnostic test in asthmatic children with or without allergic rhinitis, and whether it had any effects on pulmonary function tests and arterial oxygen saturation (sao2) values. therefore, 25 asthmatic outpatients sensitive to dermatophagoides pteronyssinus (dp), aged 6-17 years, 12 having allergic rhinitis, and 10 nonatopic children as a control group were challenged intranasally with solution contai ... | 1995 | 8546272 |
| [nasal smear cytology in bronchial asthma: correlation of appearance and numbers of nasal smear mast cells, eosinophils or basophils and serum ige antibodies to house dust mite in patients with bronchial asthma]. | nasal smear cytology was studied in pediatric patients with bronchial asthma with special reference with ige rast to house dust mite, dermatophagoides pteronyssinus (dp). results obtained were as follows: (1) numbers of eosinophils on the nasal smear correlated well with dp rast score, (2) mast cells were detected before dp rast becoming positive and appearance of eosinophils, (3) basophils appeared after detection of eosinophils and only in patients with nasal smear eosinophils. these results s ... | 1995 | 8534207 |
| no relationship between skin-infiltrating th2-like cells and allergen-specific ige response in atopic dermatitis. | more than 500 cd4+ t-cell clones (tccs) derived from the skin of eight patients with atopic dermatitis (ad), two patients with nonatopic dermatologic disorders, two patients with allergic rhinitis, and one healthy nonatopic donor were analyzed for both their pattern of cytokine production and their antigen specificity. the proportions of tccs from patients with ad producing interleukin-4 in response to stimulation with phorbol 12-myristate 13-acetate plus anti-cd3 antibody were higher, whereas t ... | 1995 | 7560644 |
| [comparison of study results for levels of specific antibodies using the rast-ige method and specific bronchial provocation in children with asthma]. | the aim of the study was to evaluate the diagnostic power of specific-ige evaluation in diagnosis of allergy to house dust mite (dermatophagoides pteronyssinus, d.pt.), in relation to specific bronchial challenge. in a group of 99 children with perennial asthma, specific-ige evaluation and bronchial challenge were performed with d.pt. allergens. the bronchial challenge was positive in 52 cases (53%). rast results were concordant with bronchial challenge from 61% (for classes > or = 1) to 75% (fo ... | 1995 | 8668365 |
| effect of an asthmatic attack on cd23 and cd21 expression on lymphocytes from allergic children during the allergen season. | the overproduction of ige antibodies by atopic individuals in response to inhaled aeroallergen, forms the basis of an allergic disease. furthermore, the exposure to allergen might trigger the symptom exacerbation. | 1995 | 7584679 |
| evaluation of the inhibitory effects of budesonide on the mitogen-induced or the allergen-induced activation of blood mononuclear cells isolated from asthmatic patients. | corticosteroids are thought to be effective in the treatment of allergic reactions including bronchial asthma because they not only have anti-inflammatory effects, but also downregulate the processes of t-cell activation. | 1995 | 7621058 |
| [nd:yag laser surgery in treatment of allergic rhinitis]. | the purpose of our study was to evaluate the effects of laser turbinectomy on local allergic inflammation by measuring the secretion of mediators (histamine, bradykinin, and tame-esterase activity) in nasal lavage fluid after nasal provocation with different allergen concentrations. our study included fifteen patients, aged fifteen to 35, who displayed perennial house-dust-mite rhinitis (positive prick test, rast class > 2 and positive nasal provocation with dermatophagoides pteronyssinus [d.p.] ... | 1995 | 7545400 |
| allergen-stimulated t lymphocytes from allergic patients induce vascular cell adhesion molecule-1 (vcam-1) expression and il-6 production by endothelial cells. | adhesion of inflammatory cells to endothelium is a critical step for their transvascular migration to inflammatory sites. to evaluate the relationship between t lymphocytes (tl) and vascular endothelium, supernatants from allergen-stimulated tl obtained from patients sensitive to dermatophagoides pteronyssinus (dpt) versus healthy subjects were added to endothelial cell (ec) cultures. tl were stimulated by autologous-activated antigen-presenting cells (apc) previously fixed in paraformaldehyde t ... | 1995 | 7542574 |
| house dust mite allergen levels in dust from schools with smooth and carpeted classroom floors. | a study was conducted to investigate the levels of house dust mite allergen der p i in classroom floor dust from 49 schools with smooth (n = 18) or carpeted (n = 31) floors. schools were located in the city of rotterdam (n = 29) and in a number of small communities in the province of gelderland (n = 20). data on building characteristics of the schools were obtained. dust samples were analysed for der p i content, the major allergen of the house dust mite dermatophagoides pteronyssinus. the resul ... | 1995 | 7648462 |
| airway response of asthmatic subjects to inhaled allergen after exposure to pollutants. | recent studies have suggested that air pollutants resulting from vehicle exhaust emissions and burning of fossil fuels, either in combination or individually, may enhance the airway response of asthmatic subjects to inhaled allergen. it was hypothesised that the airway response to inhaled allergen after exposure to a combination of 400 ppb nitrogen dioxide (no2) and 200 ppb sulphur dioxide (so2) is increased 24-48 hours after exposure. | 1996 | 8958893 |
| clinical evaluation of a new quantitative method for specific ige antibodies. | we studied the analytical and clinical efficiency of a "second generation" technique for the in vitro determination of specific ige, the so-called carla system (capture assay radim liquid allergen). reproducibility studies demonstrated satisfactory intra- and inter-assay analytical imprecision. the linearity test gave good results, and no significant interference by non-specific total ige was found. the diagnostic efficiency of the system for the five most representative aeroallergens (d1, derma ... | 1996 | 8864410 |
| measles and atopy in guinea-bissau. | epidemiological studies have led to speculation that infections in early childhood may prevent allergic sensitisation but evidence to support this hypothesis is lacking. we investigated whether measles infection protects against the development of atopy in children of guinea-bissau, west africa. | 1996 | 8667923 |
| [efficacy of levocabastine in perennial rhinitis]. | we have evaluated the efficacy of topical levocabastine in 42 patients (20 males and 22 females; mean age 37.5 yrs.) affected by perennial allergic rhinoconjunctivitis due to dermatophagoides pteronyssinus. diagnosis was performed on the basis of clinical history, skin prick test and rast. the study lasted for 90 days and nasal spray levocabastine was administered at the dose of 2 puff in each nostril twice a day. patients had to record the severity of the symptoms considered (sneezing, nasal pr ... | 1996 | 8925638 |
| immunotherapy with a standardized dermatophagoides pteronyssinus extract. v. duration of the efficacy of immunotherapy after its cessation. | specific immunotherapy (sit) using a standardized mite extract is effective and safe when administered under optimal conditions. however, the duration of its effectiveness after cessation of treatment remains unknown. forty asthmatic subjects who had received sit with a standardized dermatophagoides pteronyssinus (der p) extract under the same protocol were studied. all had received sit for a period of 12-96 months and were not receiving pharmacologic treatment. the fev1 was within normal range ... | 1996 | 8837669 |
| infant respiratory symptoms in relation to mite allergen exposure. | the relationship between the dermatophagoides pteronyssinus (der p) i content of house dust and the respiratory symptoms reported for young infants was studied. one hundred and four infants, aged 3-15 months, were selected during july-september 1993 through the dutch postnatal health care service, using a short screening questionnaire to identify mothers with respiratory allergy to house dust and/or pets. forty-eight were selected from this group of mothers ("high risk" infants) and 56 infants w ... | 1996 | 8793453 |
| sequence polymorphisms of the der p 3 house dust mite allergen. | the trypsin-like protein der p 3 is a major allergen of dermatophagoides pteronyssinus. like other vertebrate and invertebrate trypsin-like molecules, isoelectric-focusing studies with the natural der p 3 protein have indicated that several isoforms exist. | 1996 | 8735870 |
| hla-dq8 transgenic mice lacking endogenous class ii molecules respond to house dust allergens: identification of antigenic epitopes. | we have introduced hla-dq8 (hladqb*0302 and hla-dqa*0301) genes into a beta 0 knockout mice. transgenic animals were immunized with a whole body extract of dermatophagoides pteronyssinus (der p), one of the causative agents of house dust mite allergy. transgenic mice expressing hla-dq8 genes elicited hla-dq8-restricted responses driven by cd4+ t cells. synthetic-overlapping peptides representing a major allergen of house dust mite (der p 2) were synthesized and used as immunogens. hla-dq8+ mice ... | 1996 | 8617940 |
| the house dust mite allergen der p1 catalytically inactivates alpha 1-antitrypsin by specific reactive centre loop cleavage: a mechanism that promotes airway inflammation and asthma. | der p1, a cysteine proteinase derived from the house dust mite (hdm) dermatophagoides pteronyssinus, is a major component of the allergic immune response in hdm atopic individuals. recent evidence suggests that cysteine proteinase activity is important in the disease process as it increases the permeability of the allergen in the respiratory tract and disrupts the regulation of ige synthesis. der p1 is found in high concentrations in the faecal pellets of mites which are aerosolised and inhaled ... | 1996 | 8660343 |
| effects of immunotherapy on symptoms, pefr, spirometry, and airway responsiveness in patients with allergic asthma to house-dust mites (d. pteronyssinus) on inhaled steroid therapy. | the present study was designed to investigate the effects of immunotherapy (it) with an extract of dermatophagoides pteronyssinus (alergo-merck depot) during a 27-month period in patients with allergic asthma to house-dust mites. we included 11 patients (mean age 18 years) treated with a combination of it and inhaled beclomethasone dipropionate (bdp) in comparison to another 11 (mean age 22 years) treated with bdp alone. we evaluated symptom scores, salbutamol use, peak expiratory flow rates (pe ... | 1996 | 8792920 |
| interaction of allergens from house-dust mite and from cereal flours: dermatophagoides pteronyssinus alpha-amylase (der p 4) and wheat and rye alpha-amylase inhibitors. | major wheat and rye allergens associated with baker's asthma have been identified as specific inhibitors of dermatophagoides pteronyssinus alpha-amylase (der p 4). the most active inhibitors were the wheat tetrameric one and the rye dimeric rdai-1. these data suggest that interaction between mite and cereal allergens (alpha-amylase/inhibitor complexes) might occur in house-dust-mite-infected flours. in contrast to the strong inhibition of the d. pteronyssinus enzyme by the wheat and rye inhibito ... | 1996 | 8781672 |
| skin prick test reactivity to common aeroallergens in relation to total ige, respiratory symptoms, and smoking in a general population sample of northern italy. | skin prick test (spt) reactivity to common airborne allergens and its relationships to sex, age, smoking habits, and respiratory symptoms/diseases were evaluated in a general population sample (n = 2841, 8-75 years of age) living in the po delta area (northern italy). subjects completed a standardized questionnaire and underwent prick tests (12 local allergens, a negative and a positive control) and determination of total serum ige. atopy was evaluated by measuring the maximal diameter for each ... | 1996 | 8781668 |
| does laser turbinectomy influence local allergic inflammation in the nose? | the purpose of our study was to evaluate the effects of laser turbinectomy on local allergic inflammation by measuring the secretion of mediators (histamine, bradykinin, and tame-esterase activity) in nasal lavage fluid after nasal provocation with different allergen concentrations. our study included 15 patients, aged 15-35 years, who displayed perennial housedust-mite rhinitis (positive prick test, rast class > 2, and positive nasal provocation with dermatophagoides pteronyssinus (d.p.) extrac ... | 1996 | 8739871 |
| exposure chamber for allergen challenge. the development and validation of a new concept. | exposure chambers have proven to be valuable tools in studying allergic diseases. the chamber provides a controlled environment and maintains conditions for measuring the amount of allergen inducing symptoms in allergic subjects. the aim of the present study was to develop and test an exposure chamber. the chamber was constructed as an airtight tent, made of transparent polyethylene, easy to adapt to the shape of an existing room, easy to clean, and providing exact allergen-dosage control. airfl ... | 1996 | 8738512 |
| ige and igg4 antibodies from patients with mite allergy recognize different epitopes of dermatophagoides pteronyssinus group ii antigen (der p2). | der p 2, composed of 129 amino acids, is one of the major allergens of dermatophagoides pteronyssinus. several groups reported epitope mapping of ige antibody but not of igg or igg subclass antibodies in der p 2. | 1996 | 8621849 |
| increased allergen-specific, steroid-sensitive gamma delta t cells in bronchoalveolar lavage fluid from patients with asthma. | to test the hypothesis that allergen-specific, steroid-sensitive gamma delta t lymphocytes are increased in bronchoalveolar lavage fluid of patients with asthma. | 1996 | 8533997 |
| [immunologic response in patients with atopic dermatitis]. | it is a well known fact that atopic dermatitis, as one of the clinical manifestations of atopic syndrome, is characterized by a number of immunologic disorders. in 17 patients with atopic dermatitis we examined: systemic cellular immunity, production of total serum ige, specific ige to certain aero-allergens, cutaneous hypersensitivity of an early type to aero-allergens and chemotaxis activity of neutrophils and monocytes. increased production of ige proved to be the main disorder which confirms ... | 1996 | 8926949 |
| effect of cetirizine and prednisolone on cellular infiltration and cytokine mrna expression during allergen-induced late cutaneous responses. | the ability of cetirizine to inhibit eosinophil infiltration into the sites of allergen-induced cutaneous late-phase reactions is controversial. a previous skin biopsy study gave negative results with 15 mg of cetirizine as a single dose. | 1996 | 8789545 |
| differences in titres of ige, igg4 and other igg subclass anti-der p 2 antibodies in allergic and non-allergic patients measured with recombinant allergen. | the mite allergens are recognized as major causes of allergic disease such as bronchial asthma, allergic rhinitis and atopic dermatitis. the functions of allergen-specific igg subclass antibodies are not defined. | 1996 | 8789542 |
| effect of local allergen priming on early, late, delayed-phase, and epicutaneous skin reactions. | allergic disease is reflected in a chronic inflammatory response to an allergen. it is thought that local allergen priming underlies this chronicity. to assess the effect of allergen priming on the amplitude and histologic effect of the allergic reaction, four sequential, intracutaneous skin tests were done with 48-h intervals in 13 patients allergic to the house-dust mite dermatophagoides pteronyssinus (dpt). reactions were measured at 15 min, and at 6, 24, and 48 h. subsequently, epicutaneous ... | 1996 | 9020415 |
| does benzyl benzoate prevent colonization of new mattresses by mites? a prospective study. | in 12 house-dust-mite-infested double beds, one of the mattresses was replaced by a new one, the other being regarded as a mite source. all new mattresses were treated in a double-blind fashion, with either benzyl benzoate or placebo before being placed on the bed as well as 1 year later. they were examined for mites and allergen concentrations over a period of 18 months. this period of time covered two mite seasons. dust samples were taken bimonthly and analyzed by guanine test strip, microscop ... | 1996 | 9020414 |
| severity of asthma is related to endotoxin in house dust. | in sensitized subjects, exposure to the mite allergen appears to be only one of several factors leading to asthma. we hypothesized that in association with allergen exposure, endotoxin, a proinflammatory agent present in house dust (hd), influences the severity of asthma. using a cross-sectional study design, we investigated a group of 69 consecutive dust mite (hdm)-sensitized subjects defined as having rhinitis (n = 20) or asthma (n = 49); the latter were evaluated functionally and clinically b ... | 1996 | 8970348 |
| allergen specificity of skin-infiltrating t cells is not restricted to a type-2 cytokine pattern in chronic skin lesions of atopic dermatitis. | the majority of allergen-specific t cells derived from inhalant allergen patch test lesions in patients with atopic dermatitis were previously found to produce a restricted type-2 cytokine pattern. recent studies, however, have revealed that in chronic eczematous skin lesions of patients with atopic dermatitis, expression of the type-1 cytokine interferon-gamma predominates. to evaluate cytokine production by allergen-specific t cells in chronic atopic dermatitis, we established house dust mite ... | 1996 | 8941677 |
| cloning and sequencing of the group 6 allergen of dermatophagoides pteronyssinus. | the allergen der p 6 from dermatophagoides pteronyssinus has been described by substrate affinity as mite chymotrypsin. | 1996 | 8911700 |
| abnormal production of t helper 2 cytokines interleukin-4 and interleukin-5 by t cells from newborns with atopic parents. | t cell clones were generated from umbelical cord blood lymphocytes (ucbl) of nine newborns with atopic or nonatopic parents and their cytokine secretion profile was assessed. both phytohemagglutinin-induced and dermatophagoides pteronyssinus-specific t cell clones from newborns with atopic parents exhibited an enhanced ability to produce the th2 cytokines interleukin (il)-4 and il-5, compared to t cell clones from newborns with nonatopic parents. in contrast, the ability to produce interferon-ga ... | 1996 | 8898936 |
| the isolation and characterization of a novel collagenolytic serine protease allergen (der p 9) from the dust mite dermatophagoides pteronyssinus. | dust mites have been shown to contain a serine protease distinct from the previously reported trypsin and chymotrypsin. the latter enzymes have been shown to be allergens, but the allergenic importance of the former is unknown. | 1996 | 8876548 |
| inhibition of specific ige response in vivo by allergen-gene transfer. | dna immunization has been an attractive approach in altering the host immune response to antigen. to examine the utility of dna immunization in allergic response, we examined the in vivo efficacy of an 'allergen-gene immunization' approach in the modulation of allergen-specific ige responses in mice. our results showed first that i.m. injection of a gene construct (pcmvd) containing an important house dust mite allergen gene (dermatophagoides pteronyssinus group 5 allergen; der p 5) results in t ... | 1996 | 8921418 |
| indoor mite allergens in patients with respiratory allergy living in porto, portugal. | we investigated the levels of mite allergens (der p 1, der f 1, der 2, and lep d 1) in dust samples from the homes of 59 patients with asthma, 36 sensitized to house-dust mites (hdm) and 23 to grass pollen (controls), living in porto, northern portugal. the relationship between exposure and sensitization to hdm and the influence of housing conditions on mite-allergen levels were also evaluated. der p 1 (median 9.2 micrograms/g) and der 2 (4.6 micrograms/g) were the main allergens, while der f 1 ... | 1996 | 8899115 |
| long-term oral corticosteroid therapy does not alter the results of immediate-type allergy skin prick tests. | medications can modulate the results of skin prick tests (spts). short-term corticosteroid therapy does not alter ige-mediated skin tests, but the impact of long-term oral corticosteroid therapy on spt results is unclear. a prospective study was carried out in patients with steroid-dependent asthma who received oral corticosteroids for a long period to determine whether this treatment reduced skin test reactivity. | 1996 | 8828529 |
| mhc class ii restriction for t cell proliferative response to mite antigen. | we investigated the immunoreguratory role of the major histocompatibility complex in peripheral blood lymphocytes' proliferative response to mite antigen. | 1996 | 8814043 |
| cockroach and dermatophagoides pteronyssinus cross-react. | 1996 | 8757230 | |
| positive nasal challenge responses to blomia tropicalis. | blomia tropicalis, a dust mite commonly found in subtropical and tropical environments, is the fourth most common mite in the united states. thirty-eight percent of 167 consecutive subjects evaluated for allergic respiratory symptoms in the tampa bay area had positive skin test responses to b. tropicalis. | 1996 | 8626980 |
| use of recombinant group 5 allergens to investigate ige-mediated sensitization to blomia tropicalis and dermatophagoides pteronyssinus. | 1996 | 9095238 | |
| skin prick tests with standardized extracts of inhalant allergens in 7099 adult patients with asthma or rhinitis: cross-sensitizations and relationships to age, sex, month of birth and year of testing. | the results of skin prick tests (spts) performed between 1981 and 1992 on 7099 adult patients with asthma and/or rhinitis were retrospectively analyzed. standardized soluprick extracts of dermatophagoides pteronyssinus, animals and pollen, and unstandardized extracts of cladosporium++ and chironomids (red mosquito larvae, chir), were used. the proportion of atopics (patients with positive spt results) was 44 percent, decreasing from 61 percent in patients 14-20 years old to 18 percent in patient ... | 1996 | 8833167 |
| [allergenic mites found in raw material of herbs based on literature about the pyroglyphidae (acari: acaridida)]. | the occurrence of two pyroglyphid mites. euroglyphus maynei (cooreman, 1950) and dermatophagoides pteronyssinus (trouessart, 1897) in the raw material of herbs is discussed and compared with the literature data. these mites are considered to be the cause of human atopic diseases. | 1996 | 8755152 |
| allergen-specific ige levels and mite allergen exposure in children with acute asthma first seen in an emergency department and in nonasthmatic control subjects. | sensitization to allergens has been shown to be a risk factor for adults with acute asthma first seen in the emergency department. | 1996 | 8757201 |
| absence of continuous epitopes in the house dust mite major allergens der p i from dermatophagoides pteronyssinus and der f i from dermatophagoides farinae. | the house dust mite has been shown to be an important source of domestic allergens associated with immediate hypersensitivities. the group i mite allergens der p i from dermatophagoides pteronyssinus and der f i from d. farinae display extensive amino acid sequence homology and have similarities with cysteine protease enzymes. | 1996 | 8789541 |
| monoclonal antibodies against dermatophagoides group i allergens as pseudo-cystatins blocking the catalytic site of cysteine proteinases. | the enzyme allergens der p i and der f i produced by the house dust mites dermatophagoides pteronyssinus and d. farinae display partial sequence homology with other members of the cysteine proteinase superfamily. we report that certain widely used mouse mabs against these group i allergens indeed crossreact with the plant enzymes papain, bromelain and ficin. the recognition sites of these anti group i mabs comprise conformational and thermolabile epitopes involved in molding the catalytic center ... | 1996 | 8803616 |
| eosinophil locomotion and the release of il-3 and il-5 by allergen-stimulated mononuclear cells are effectively downregulated in vitro by budesonide. | treatment of allergic asthma with inhaled corticosteroids, such as budesonide (bdn), results in downregulation of t-cell activation and of eosinophil recruitment. | 1996 | 8809423 |
| the significance of reactions to purified fractions of dermatophagoides pteronyssinus and dermatophagoides farinae in canine atopic dermatitis. | the significance of reactions to crude extracts and purified fractions of the house dust mites dermatophagoides pteronyssinus (der p i and der p ii) and dermatophagoides farinae (der f i and der f ii) was evaluated in dogs with clinical manifestations of atopic dermatitis (ad). in 13 healthy control dogs and eight dogs with ad, immediate skin test reactivity was determined to serial dilutions of der p i, der p ii, der f i and der f ii. in addition, allergen-specific iggd antibodies were determin ... | 1996 | 8809997 |
| [the isolation and characteristics of a chemically modified allergen (allergoid) from dermatophagoides pteronyssinus mites]. | chemically modified allergens (allergoids) were obtained from house dust mites (dermatophagoides pteronyssinus). the allergenicity of allergoids in comparison with commercial mite allergen was determined by the skin prick test and by the in vitro test of degranulation of passively sensitized rat mast cells. changes in allergoid molecules were determined with the use of gel filtration in a column packed with sephadex g-75, isoelectric focusing in polyacrylamide gel, the determination of the conce ... | 1996 | 9103081 |
| epitope analysis of hla-dr-restricted helper t-cell responses to der p ii, a major allergen molecule of dermatophagoides pteronyssinus. | t-cell epitopes of der p ii, a major allergen of dermatophagoides pteronyssinus, were analyzed by using human t-cell clones. we tested 38 cloned t cells from two japanese patients with allergic rhinitis, and identified at least two peptides (k33-t47 and i58-c73) as helper t-cell epitopes. the former epitope was shown to be restricted by hla-drb1*1502, and the latter by hla-drb1*0405, both of which are typical japanese hla-dr alleles, suggesting that those t-cell epitopes might be important for t ... | 1996 | 8721525 |
| immunologic responses following respiratory sensitization to house dust mite allergens in mice. | allergens from the house dust mite dermatophagoides pteronyssinus are a major cause of human respiratory diseases, including asthma. in order to help in understanding the early events in allergen sensitization, a murine model of allergic respiratory disease was developed. mice were immunized by intranasal inoculation of der p 1 or der p 2 on days 0, 3, 7, 10, 14, 17, 21 and 29. t cell reactivity was determined using in vitro assays of allergen-specific cytokine production by cells from the splee ... | 1996 | 8724006 |
| an in vivo model of allergic inflammation: pulmonary human cell infiltrate in allergen-challenged allergic hu-scid mice. | cb.17 severe combined immunodeficient (scid) mice were used to establish a model of allergic pulmonary inflammation. scid mice were intraperitoneally reconstituted with 10(7) peripheral blood mononuclear cells (pbmc) from patients sensitive to dermatophagoides pteronyssinus (dpt) and 2 weeks later were exposed to dpt aerosols. after dpt nebulization, scid mice engrafted with pbmc from dpt-sensitive patients developed a specific human ige response as well as an intense pulmonary infiltrate of hum ... | 1996 | 8647172 |
| the sweat of patients with atopic dermatitis contains specific ige antibodies to inhalant allergens. | we have investigated levels of total and specific ige against inhalant allergens in the sweat of 15 patients with atopic dermatitis, 10 patients with allergic rhinitis and high levels of specific ige in the serum, and five patients with psoriasis without atopy as controls, by means of various commercial methods such as fluorescence immunoassay, nephelometry, chemiluminescence assay, enzyme immunoassay and the radioallergosorbent test. total ige and specific ige antibodies were detectable in the ... | 1996 | 9136153 |
| receiver-operating characteristic (roc) curves: a fundamental tool for improving the clinical usefulness of in vitro ige tests. | in order to establish the most efficient thresholds for serum allergen-specific ige measured by the pharmacia cap system, a "second-generation" in vitro method, we evaluated results from 89 subjects with suspected inhalant allergies, using receiver-operating characteristic (roc) curve analysis. sera samples were analyzed by the pharmacia cap system for specific ige, the choice of allergens to be tested being based upon the symptoms and clinical history of each patient. results were analyzed by r ... | 1996 | 8837664 |
| sensitization to dermatophagoides siboney, blomia tropicalis, and other domestic mites in asthmatic patients. | mite species adapted to warm, humid climates are commonly found in house dust in the tropics. in cuba, dermatophagoides pteronyssinus, d. siboney, and blomia tropicalis are the most common and abundant mite species in house dust. to investigate the pattern of sensitization of cuban asthmatic patients to common mite species, we skin-prick-tested (spt) 148 patients with a clinical history of asthma and possible mite allergy, and determined specific ige antibodies against mite allergens (d. pterony ... | 1996 | 8863927 |
| transient sensitization to house-dust mites: a study on the influence of mite exposure and sex. | this study aimed to evaluate the prognosis of a previous positive skin test to house-dust mites (hdm) in relation to environmental exposure. a total of 115 children, 50 from stockholm and 65 from northern sweden, all with a previous (average 2.5 years) positive phazet (pharmacia ab, uppsala, sweden) skin prick test (spt) to extracts of dermatophagoides pteronyssinus (dpt) and/or d. farinae (df) were included. dust samples were collected from the children's mattresses, and the total (dpt, df, and ... | 1996 | 8863929 |
| determination of the stability of diluted allergen extracts using a concentration step prior to east inhibition. | generally the stability of diluted allergen extracts, as used for skin testing, provocation testing and immunotherapy can not be measured using a normal enzyme allergosorbent test (east) inhibition method. | 1996 | 8877162 |
| respiratory allergy to parietaria pollen in 348 subjects. | we have revisited 348 records of allergic outpatients to parietaria, ratio m/f 1:1 and mean age 29.46 +/- 12.49 yrs. 97 of them were skin positive to parietaria only (a-group: m/f 37/60); 251 patients were skin positive also to other allergens (b-group: m/f 137/114). mean age of a-group patients (35.41 +/- 12.91) was higher than b-group (27.16 +/- 11.54; p < 001). dividing mono and polysensitized subjects by age decade, a different distribution was found between the two groups (p < 001): monosen ... | 1996 | 8882753 |
| childhood asthma and exposure to indoor allergens: low mite levels are associated with sensitivity. | the prevalence and level of sensitivity to indoor allergens were studied in relation to current exposure at home in 124 children with perennial asthma living in three climatic zones of sweden. the house dust mite (hdm) allergen levels were higher in the south than in the north (p < 0.001), while cat and dog allergen levels tended to be higher in the north than the south (n.s.). thirty-four percent of the children were sensitive to the hdm dermatophagoides pteronyssinus, as determined by ige anti ... | 1996 | 8902855 |
| the disturbance of epidermal barrier function in atopy patch test reactions in atopic eczema. | atopic eczema (ae) is a common skin disorder. eczematous lesions showing macroscopic, microscopic and immunopathological resemblance to lesional ae can be induced by aeroallergens by epicutaneous testing (atopy patch test, apt). altered epidermal barrier function, as determined by transepidermal water loss (tewl), is a typical feature of patients with ae. the present investigation was performed to define the differences in the epidermal barrier function between positive apt reactions to aeroalle ... | 1996 | 8915146 |
| interaction of human lung surfactant proteins a and d with mite (dermatophagoides pteronyssinus) allergens. | human lung surfactant proteins a (sp-a) and d (sp-d) are both collagenous c-type lectins which appear to mediate antimicrobial activity by binding to carbohydrates on micro-organisms and to receptors on phagocytic cells. purified native sp-a and sp-d, isolated from human bronchoalveolar lavage fluid, were found to bind to whole mite extracts (dermatophagoides pteronyssinus) and the purified allergen der p i, in a carbohydrate-specific and calcium-dependent manner. binding was inhibited by ethyle ... | 1996 | 8918587 |