Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| peptaibol, secondary-metabolite, and hydrophobin pattern of commercial biocontrol agents formulated with species of the trichoderma harzianum complex. | the production of bioactive polypeptides (peptaibiotics) in vivo is a sophisticated adaptation strategy of both mycoparasitic and saprotrophic trichoderma species for colonizing and defending their natural habitats. this feature is of major practical importance, as the detection of peptaibiotics in plant-protective trichoderma species, which are successfully used against economically relevant bacterial and fungal plant pathogens, certainly contributes to a better understanding of these complex a ... | 2015 | 25879509 |
| optimization of solid-state fermentation conditions for trichoderma harzianum using an orthogonal test. | the aim of this study was to develop a protocol for the production of fungal bio-pesticides with high efficiency, low cost, and non-polluting fermentation, while also increasing their survival rate under field conditions. this is the first study to develop biocontrol trichoderma harzianum transformants ts1 that are resistant to benzimidazole fungicides. agricultural corn stover and wheat bran waste were used as a medium and inducing carbon source for solid fermentation. spore production was obse ... | 2015 | 25867321 |
| analysis of genomic regions of trichoderma harzianum ioc-3844 related to biomass degradation. | trichoderma harzianum ioc-3844 secretes high levels of cellulolytic-active enzymes and is therefore a promising strain for use in biotechnological applications in second-generation bioethanol production. however, the t. harzianum biomass degradation mechanism has not been well explored at the genetic level. the present work investigates six genomic regions (~150 kbp each) in this fungus that are enriched with genes related to biomass conversion. a bac library consisting of 5,760 clones was const ... | 2015 | 25836973 |
| liquid culture production of microsclerotia and submerged conidia by trichoderma harzianum active against damping-off disease caused by rhizoctonia solani. | media and culturing protocols were identified that supported the formation of submerged conidia and microsclerotia (ms) by trichoderma harzianum rifai strain t-22 using liquid culture fermentation. liquid media with a higher carbon concentration (36 g l(-1)) promoted ms formation at all c:n ratios tested. hyphae aggregated to form ms after 2 d growth and after 7 d ms were fully melanized. this is the first report of ms formation by t. harzianum or any species of trichoderma. furthermore, submerg ... | 2015 | 25813507 |
| the variations of glycolysis and tca cycle intermediate levels grown in iron and copper mediums of trichoderma harzianum. | the efficiency of optimal metabolic function by microorganism depends on various parameters, especially essential metal supplementation. in the present study, the effects of iron and copper metals on metabolism were investigated by determination of glycolysis and tricarboxylic acid (tca) cycle metabolites' levels with respect to the metal concentrations and incubation period in trichoderma harzianum. the pyruvate and citrate levels of t. harzianum increased up to 15 mg/l of copper via redirectio ... | 2015 | 25805013 |
| penicillium ochrochloron mtcc 517 chitinase: an effective tool in commercial enzyme cocktail for production and regeneration of protoplasts from various fungi. | penicillium ochrochloron mtcc 517 is a potent producer of chitinolytic enzymes. novozyme 234, traditional enzyme cocktail for protoplast generation is not available in the market. so, new enzyme cocktail is prepared for protoplast formation from various filamentous fungi which consists of 5 mg ml(-1) lysing enzymes from trichoderma harzianum, 0.06 mg ml(-1) β-glucuronidase from helix pomatia and 1 mg ml(-1) p. ochrochloron chitinase. the greatest number of protoplasts could be produced from most ... | 2015 | 25737658 |
| trichoderma harzianum t-78 supplementation of compost stimulates the antioxidant defence system in melon plants. | compost is emerging as an alternative plant growing medium in efforts to achieve more sustainable agriculture. the addition of specific microorganisms such as trichoderma harzianum to plant growth substrates increases yields and reduces plant diseases, but the mechanisms of such biostimulants and the biocontrol effects are not yet fully understood. in this work we investigated how the addition of citrus and vineyard composts, either alone or in combination with t. harzianum t-78, affects the ant ... | 2015 | 25255983 |
| comparative evaluation of two trichoderma harzianum strains for major secondary metabolite production and antifungal activity. | this investigation was undertaken to identify the major secondary metabolite, produced by two trichoderma harzianum strains (t-4 and t-5) with their antifungal activity against phytopathogenic fungi using poison food technique. the ethyl acetate extract was subjected to column chromatography using n-hexane, ethyl acetate and methanol gradually. chromatographic separation of ethyl acetate extract of t. harzianum (t-4) resulted in the isolation and identification of palmitic acid (1), 1,8-dihydrox ... | 2015 | 25248548 |
| validation of a novel sequential cultivation method for the production of enzymatic cocktails from trichoderma strains. | the development of new cost-effective bioprocesses for the production of cellulolytic enzymes is needed in order to ensure that the conversion of biomass becomes economically viable. the aim of this study was to determine whether a novel sequential solid-state and submerged fermentation method (sf) could be validated for different strains of the trichoderma genus. cultivation of the trichoderma reesei rut-c30 reference strain under sf using sugarcane bagasse as substrate was shown to be favorabl ... | 2015 | 25399068 |
| trichodiene production in a trichoderma harzianum erg1-silenced strain provides evidence of the importance of the sterol biosynthetic pathway in inducing plant defense-related gene expression. | trichoderma species are often used as biocontrol agents against plant-pathogenic fungi. a complex molecular interaction occurs among the biocontrol agent, the antagonistic fungus, and the plant. terpenes and sterols produced by the biocontrol fungus have been found to affect gene expression in both the antagonistic fungus and the plant. the terpene trichodiene (td) elicits the expression of genes related to tomato defense and to botrytis virulence. we show here that td itself is able to induce t ... | 2015 | 26168138 |
| hydrodynamics, fungal physiology, and morphology. | filamentous cultures, such as fungi and actinomycetes, contribute substantially to the pharmaceutical industry and to enzyme production, with an annual market of about 6 billion dollars. in mechanically stirred reactors, most frequently used in fermentation industry, microbial growth and metabolite productivity depend on complex interactions between hydrodynamics, oxygen transfer, and mycelial morphology. the dissipation of energy through mechanically stirring devices, either flasks or tanks, im ... | 2015 | 25652005 |
| genotype-specific variation in the structure of root fungal communities is related to chickpea plant productivity. | increasing evidence supports the existence of variations in the association of plant roots with symbiotic fungi that can improve plant growth and inhibit pathogens. however, it is unclear whether intraspecific variations in the symbiosis exist among plant cultivars and if they can be used to improve crop productivity. in this study, we determined genotype-specific variations in the association of chickpea roots with soil fungal communities and evaluated the effect of root mycota on crop producti ... | 2015 | 25616789 |
| biological control of rigidoporus lignosus in hevea brasiliensis in nigeria. | the study was conducted to evaluate in vitro and in vivo control of fungal antagonists on rigidoporus lignosus (klotzsch) imaz in hevea brasiliensis (willd. ex a. juss.) muell. arg. in the rain forest zone of nigeria. in vitro assessments of biological agents were carried out in dixenic cultures in petri plates. in vivo tests were carried out in the nursery using hypocrea virens and hypocrea jecorina. significant differences were observed with the dual inoculations of fungal antagonists and r. l ... | 2015 | 25601145 |
| integrated options for the management of black root rot of strawberry caused by rhizoctonia solani kuhn. | an investigation was made to manage strawberry black root rot caused by rhizoctonia solani (r. solani) through the integration of trichoderma harzianum (t. harzianum) isolate sta7, mustard oil cake and provax 200. a series of preliminary experiments were conducted to select a virulent isolate of r. solani, an effective isolate of t. harzianum, a suitable organic amendment, and a suitable fungicide before setting the experiment for integration. the pathogenicity of the selected four isolates of r ... | 2015 | 25595298 |
| oxidative damage induced by heat stress could be relieved by nitric oxide in trichoderma harzianum ltr-2. | trichoderma harzianum is an important commercial biocontrol fungal agent. the temperature has been shown to be an important parameter and strain-specific to the mycelia growth of fungi, but less report makes the known of the mechanisms in t. harzianum. in our study, a 6-h treatment of heat increased the thiobarbituric acid reactive substances (tbars) and nitric oxide (no) concentration in mycelia to 212 and 230 % the level of the control, respectively. the exogenous no donor sodium nitroprusside ... | 2015 | 25561405 |
| integrated effect of microbial antagonist, organic amendment and fungicide in controlling seedling mortality (rhizoctonia solani) and improving yield in pea (pisum sativum l.). | the study evaluated the comparative performance of a few microbial antagonists, organic amendments and fungicides and their integration for the management of seedling mortality (rhizoctonia solani kühn) and yield improvement in pea (pisum sativum l.). before setting the experiment in field microplots, a series of in vitro and in vivo experiments were conducted to select a virulent isolate of r. solani, an effective antagonistic isolate of trichoderma harzianum, a fungitoxic organic amendment and ... | 2015 | 25528673 |
| systemic resistance to powdery mildew in brassica napus (aacc) and raphanus alboglabra (rrcc) by trichoderma harzianum th12. | trichoderma harzianum th12 is a microbial pesticide for certain rapeseed diseases. the mechanism of systemic resistance induced by th12 or its cell-free culture filtrate (cf) in brassica napus (aacc) and raphanus alboglabra (rrcc) to powdery mildew disease caused by ascomycete erysiphe cruciferarum was investigated. in this study, we conducted the first large-scale global study on the cellular and molecular aspects of b. napus and r. alboglabra infected with e. cruciferarum. the histological stu ... | 2015 | 26540161 |
| influence of rhizoctonia solani and trichoderma spp. in growth of bean (phaseolus vulgaris l.) and in the induction of plant defense-related genes. | many trichoderma species are well-known for their ability to promote plant growth and defense. we study how the interaction of bean plants with r. solani and/or trichoderma affect the plants growth and the level of expression of defense-related genes. trichoderma isolates were evaluated in vitro for their potential to antagonize r. solani. bioassays were performed in climatic chambers and development of the plants was evaluated. the effect of trichoderma treatment and/or r. solani infection on t ... | 2015 | 26442006 |
| de novo transcriptome analyses of host-fungal interactions in oil palm (elaeis guineensis jacq.). | basal stem rot (bsr) is a fungal disease in oil palm (elaeis guineensis jacq.) which is caused by hemibiotrophic white rot fungi belonging to the ganoderma genus. molecular responses of oil palm to these pathogens are not well known although this information is crucial to strategize effective measures to eradicate bsr. in order to elucidate the molecular interactions between oil palm and g. boninense and its biocontrol fungus trichoderma harzianum, we compared the root transcriptomes of untreate ... | 2016 | 26781612 |
| degradation of dyes using crude extract and a thermostable and ph-stable laccase isolated from pleurotus nebrodensis. | three laccase isoenzymes (lac1, lac2 and lac3) have been purified to homogeneity from pleurotus nebrodensis in our previous study. lac2 was shown to be the dominant isoform, capable of oxidizing the majority of laccase substrates and manifesting good thermostability and ph stability. hence, lac2 was selected to decolourize structurally different dyes and the colour removal efficiencies of lac2 and the crude extract of p. nebrodensis were compared. by monitoring the λmax of the reaction system du ... | 2016 | 27354563 |
| anthracnose of lucky bamboo dracaena sanderiana caused by the fungus colletotrichum dracaenophilum in egypt. | dracaena sanderiana, of the family liliaceae, is among the ornamental plants most frequently imported into egypt. typical anthracnose symptoms were observed on the stems of imported d. sanderiana samples. the pathogen was isolated, demonstrated to be pathogenic based on koch's rule and identified as colletotrichum dracaenophilum. the optimum temperature for its growth ranges from 25 to 30 °c, maintained for 8 days. kemazed 50% wettable powder (wp) was the most effective fungicide against the pat ... | 2016 | 27222738 |
| biodiversity of trichoderma community in the tidal flats and wetland of southeastern china. | to investigate the biodiversity of trichoderma (hypocreaceae) and their relation to sediment physical and chemical properties, we collected a total of 491 sediment samples from coastal wetlands (tidal flat and wetland) in southeast china. further, we applied two types of molecular approaches such as culture dependent and independent methods for identification of trichoderma spp. a total of 254 isolates were obtained and identified to 13 species such as t. aureoviride, t. asperellum, t. harzianum ... | 2016 | 28002436 |
| structural and mechanistic analysis of engineered trichodiene synthase enzymes from trichoderma harzianum: towards higher catalytic activities empowering sustainable agriculture. | trichoderma spp. are well-known bioagents for the plant growth promotion and pathogen suppression. the beneficial activities of the fungus trichoderma spp. are attributed to their ability to produce and secrete certain secondary metabolites such as trichodermin that belongs to trichothecene family of molecules. the initial steps of trichodermin biosynthetic pathway in trichoderma are similar to the trichothecenes from fusarium sporotrichioides. trichodiene synthase (ts) encoded by tri5 gene in t ... | 2016 | 26207800 |
| fusant trichoderma hf9 with enhanced extracellular chitinase and protein content. | strain improvement was carried out to obtain higher chitinase and protein by inter-specific protoplast fusion between trichoderma harzianum and trichoderma viride. fusant hf9 and parental strains of trichoderma were compared for chitinase and protein production. 1% of glucose, sucrose and fungal cell wall (rhizoctonia solani), were used as carbon source for cultivation of trichoderma and fungal cell wall was the best to induce chitinase and protein. usage of 0.5% colloidal chitin for the fungal ... | 2016 | 23035579 |
| effects of different inoculum densities of trichoderma harzianum and trichoderma viride against meloidogyne javanica on tomato. | a greenhouse experiment was conducted to evaluate the effects of different inoculum densities of two saudi isolates of trichoderma harzianum and trichoderma viride against meloidogyne javanica on tomato. four densities (10(4), 10(6), 10(8) and 10(10) spores/g of soil) of each fungus were used. the results indicate that all four inoculum densities of the two trichoderma species suppressed the nematode reproduction and root galling; and increased the growth of tomato plants, compared to controls. ... | 2016 | 26981012 |
| bio-protective microbial agents from rhizosphere eco-systems trigger plant defense responses provide protection against sheath blight disease in rice (oryza sativa l.). | sheath blight of rice (oryza sativa l.) caused by rhizoctonia solani is a major disease and attempts are being made to develop microbe based technologies for biocontrol of this pathogen. however, the mechanisms of biocontrol are not fully understood and still require indepth study in the backdrop of emerging concepts in biological systems. the present investigation was aimed at deciphering the mechanisms of biocontrol of sheath blight of rice employing pseudomonas fluorescens and trichoderma har ... | 2016 | 27664749 |
| trichoderma l-lysine-α-oxidase producer strain culture fluid inhibits impatiens necrotic spot virus. | a method for pcr diagnosis of impatiens necrotic spot virus is developed. concentrated culture fluid with active l-lysine-α-oxidase (0.54 u/ml) from trichoderma harzianum rifai fungus producer strain f-180 inhibits vitally hazardous impatiens necrotic spot phytovirus. | 2016 | 26742733 |
| secretome analysis of the mycoparasitic fungus trichoderma harzianum all 42 cultivated in different media supplemented with fusarium solani cell wall or glucose. | trichoderma harzianum is a fungus well known for its potential as a biocontrol agent against many fungal phytopathogens. the aim of this study was to characterize the proteins secreted by t. harzianum all42 when its spores were inoculated and incubated for 48 h in culture media supplemented with glucose (glu) or with cell walls from fusarium solani (fscw), a phytopathogen that causes severe losses in common bean and soy crops in brazil, as well as other crop diseases around the world. trichoderm ... | 2016 | 26631988 |
| native isolate of trichoderma: a biocontrol agent with unique stress tolerance properties. | species of trichoderma are widely recognized for their biocontrol abilities, but seldom studied collectively, for their plant growth promotion, abiotic stress tolerance and bioremediation properties. our study is a concentrated effort to establish the potential of native isolate trichoderma harzianum ksnm (t103) to tolerate biotic (root pathogens) and abiotic stresses [high salt (100-1000 mm); heavy metal (chromium, nickel and zinc: 1-10 mm); pesticides: malathion (100-600 ppm), carbofuran (100- ... | 2016 | 27339311 |
| marine fungi isolated from chilean fjord sediments can degrade oxytetracycline. | salmon farming is the main economic activity in the fjords area of southern chile. this activity requires the use of antibiotics, such as oxytetracycline, for the control and prevention of diseases, which have a negative impact on the environment. we analyzed the abilities of endemic marine fungi to biodegrade oxytetracycline, an antibiotic used extensively in fish farming. we isolated marine fungi strains from sediment samples obtained from an area of fish farming activity. the five isolated st ... | 2016 | 27418075 |
| purification and biochemical characterization of a neutral serine protease from trichoderma harzianum. use in antibacterial peptide production from a fish by-product hydrolysate. | this study reports the purification and biochemical characterization of an extracellular neutral protease from the fungus trichoderma harzianum. the protease (th-protease) was purified from the culture supernatant to homogeneity by a three-step procedure with 14.2% recovery and 9.06-fold increase in specific activity. the purified enzyme appeared as a single protein band after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) with a molecular mass of about 20 kda. the optimum ... | 2016 | 27987188 |
| cellulase from trichoderma harzianum interacts with roots and triggers induced systemic resistance to foliar disease in maize. | trichoderma harzianum is well known to exhibit induced systemic resistance (isr) to curvularia leaf spot. we previously reported that a c6 zinc finger protein (thc6) is responsible for a major contribution to the isr to the leaf disease, but the types of effectors and the signals mediated by thc6 from trichoderma are unclear. in this work, we demonstrated that two hydrolases, thph1 and thph2, from t. harzianum were regulated by thc6. furthermore, an electrophoretic mobility shift assay (emsa) st ... | 2016 | 27830829 |
| structure and properties of native and unfolded lysing enzyme from t. harzianum: chemical and ph denaturation. | the effect of chemical denaturants and ph on the change of the conformation of the protein lysing enzyme from trichoderma harzianum has been investigated by dynamic light scattering (dls) and turbidimetry. chemical denaturants are frequently used to describe the mechanisms of folding and transition states. we have analyzed the ph effect on the properties and particle size of the protein. the compaction factor ci has shown that the protein is weakly disordered. the molecular dynamics simulations ... | 2016 | 27496603 |
| statistical culture-based strategies to enhance chlamydospore production by trichoderma harzianum sh2303 in liquid fermentation. | trichoderma-based formulations are applied as commercial biocontrol agents for soil-borne plant pathogens. chlamydospores are active propagules in trichoderma spp., but their production is currently limited due to a lack of optimal liquid fermentation technology. in this study, we explored response surface methodologies for optimizing fermentation technology in trichoderma sh2303. our initial studies, using the plackett-burman design, identified cornmeal, glycerol, and initial ph levels as the m ... | 2016 | 27487807 |
| microbial secondary metabolites ameliorate growth, in planta contents and lignification in withania somnifera (l.) dunal. | in the present investigation, metabolites of streptomyces sp. mtn14 and trichoderma harzianum thu significantly enhanced biomass yield (3.58 and 3.48 fold respectively) in comparison to the control plants. the secondary metabolites treatments also showed significant augmentation (0.75-2.25 fold) in withanolide a, a plant secondary metabolite. lignin deposition, total phenolic and flavonoid content in w. somnifera were maximally induced in treatment having t. harzianum metabolites. also, trichode ... | 2016 | 27436916 |
| the psychrotolerant antarctic fungus lecanicillium muscarium ccfee 5003: a powerful producer of cold-tolerant chitinolytic enzymes. | lecanicillium muscarium ccfee 5003, isolated in continental antarctica, is a powerful producer of extracellular cold-tolerant enzymes. chitin-hydrolyzing enzymes seems to be the principal extracellular catalytic activities of this psychrotolerant fungus. the production of chitinolytic activities is induced by chitin and other polysaccharides and is submitted to catabolite repression. the chitinolytic system of l. muscarium consists of a number of different proteins having various molecular weigh ... | 2016 | 27058517 |
| biodegradation of carbamazepine and clarithromycin by trichoderma harzianum and pleurotus ostreatus investigated by liquid chromatography - high-resolution tandem mass spectrometry (fticr ms-irmpd). | in this study, the capability of pharmaceutical biodegradation of fungus trichoderma harzianum was evaluated through the comparison with the well-known biodegradation capability of white-rot fungus pleurotus ostreatus. the study was performed in aqueous phase under aerobic conditions, using two of the most frequently detected drugs in water bodies: carbamazepine and clarithromycin, with concentrations commonly found in treated wastewater (4μg/l and 0.03μg/l respectively). for the first time, we ... | 2016 | 27039063 |
| phosphoproteomic analysis of induced resistance reveals activation of signal transduction processes by beneficial and pathogenic interaction in grapevine. | protein phosphorylation regulates several key processes of the plant immune system. protein kinases and phosphatases are pivotal regulators of defense mechanisms elicited by resistance inducers. however, the phosphorylation cascades that trigger the induced resistance mechanisms in plants have not yet been deeply investigated. the beneficial fungus trichoderma harzianum t39 (t39) induces resistance against grapevine downy mildew (plasmopara viticola), but its efficacy could be further improved b ... | 2016 | 27010348 |
| trichoderma harzianum produces a new thermally stable acid phosphatase, with potential for biotechnological application. | acid phosphatases (acpases) are produced by a variety of fungi and have gained attention due their biotechnological potential in industrial, diagnosis and bioremediation processes. these enzymes play a specific role in scavenging, mobilization and acquisition of phosphate, enhancing soil fertility and plant growth. in this study, a new acpase from trichoderma harzianum, named acpase ii, was purified and characterized as a glycoprotein belonging to the acid phosphatase family. acpase ii presents ... | 2016 | 26938873 |
| isolation and identification of trichoderma harzianum from groundwater: an effective biosorbent for defluoridation of groundwater. | the ability of non-viable form of trichoderma harzianum, isolated from fluoride rich groundwater, was investigated as biosorbent for defluoridation of groundwater. biosorption experiments were carried out at laboratory scale for removal of fluoride from groundwater. significant effect of operational parameters on fluoride biosorption using trichoderma harzianum as biosorbent was evaluated by varying operational parameters such as: initial fluoride concentration (2-8 mgl(-1)), biosorbent dose (0. ... | 2016 | 26930871 |
| dose-dependent response of trichoderma harzianum in improving drought tolerance in rice genotypes. | this study demonstrates a dose-dependent response of trichoderma harzianum th-56 in improving drought tolerance in rice by modulating proline, sod, lipid peroxidation product and dhn / aqu transcript level, and the growth attributes. in the present study, the effect of colonization of different doses of t. harzianum th-56 strain in rice genotypes were evaluated under drought stress. the rice genotypes treated with increasing dose of t. harzianum strain th-56 showed better drought tolerance as co ... | 2016 | 26898554 |
| enhanced cellulase production by trichoderma harzianum by cultivation on glycerol followed by induction on cellulosic substrates. | the use of glycerol obtained as an intermediate of the biodiesel manufacturing process as carbon source for microbial growth is a potential alternative strategy for the production of enzymes and other high-value bioproducts. this work evaluates the production of cellulase enzymes using glycerol for high cell density growth of trichoderma harzianum followed by induction with a cellulosic material. firstly, the influence of the carbon source used in the pre-culture step was investigated in terms o ... | 2016 | 26883662 |
| selection and characterization of argentine isolates of trichoderma harzianum for effective biocontrol of septoria leaf blotch of wheat. | species of the genus trichoderma are economically important as biocontrol agents, serving as a potential alternative to chemical control. the applicability of trichoderma isolates to different ecozones will depend on the behavior of the strains selected from each zone. the present study was undertaken to isolate biocontrol populations of trichoderma spp. from the argentine wheat regions and to select and characterize the best strains of trichoderma harzianum by means of molecular techniques. a t ... | 2016 | 26873560 |
| elicitors' influenced differential ginsenoside production and exudation into medium with concurrent rg3/rh2 panaxadiol induction in panax quinquefolius cell suspensions. | cobalt nitrate, nickel sulphate, hydrogen peroxide, sodium nitroprusside, and culture filtrates of pseudomonas monteili, bacillus circularans, trichoderma atroviridae, and trichoderma harzianum were tested to elicit ginsenoside production in a cell suspension line of panax quinquefolius. abiotic elicitors preferentially increased panaxadiols whereas biotic elicitors upregulated the panaxatriol synthesis. cobalt nitrate (50 μm) increased total ginsenosides content by twofold (54.3 mg/l) within 5 ... | 2016 | 26795963 |
| biocontrol potential of trichoderma harzianum isolate t-aloe against sclerotinia sclerotiorum in soybean. | sclerotinia stem rot, caused by sclerotinia sclerotiorum (lib.) de bary is a major disease of soybean (glycine max (l.) merr.). at present, we revealed the three-way interaction between trichoderma harzianum t-aloe, pathogen s. sclerotiorum and soybean plants in order to demonstrate biocontrol mechanism and evaluate biocontrol potential of t-aloe against s. sclerotiorum in soybean. in our experiments, t-aloe inhibited the growth of s. sclerotiorum with an efficiency of 56.3% in dual culture test ... | 2016 | 26774866 |
| development of a multiplex q-pcr to detect trichoderma harzianum rifai strain t22 in plant roots. | the fungal species trichoderma harzianum is widely used as a biological agent in crop protection. to verify the continued presence of this fungus on plant roots manually inoculated with t. harzianum strain t22, a q-pcr was designed using specific probes for this particular strain. to develop these molecular diagnostic tools, genome mining was first carried out to retrieve putative new regions by which different strains of t. harzianum could be distinguished. subsequently, sanger sequencing of th ... | 2016 | 26747625 |
| purification and characterization of endo β-1,4-d-glucanase from trichoderma harzianum strain hzn11 and its application in production of bioethanol from sweet sorghum bagasse. | an acidophilic-solvent-thermostable endo β-1,4-d-glucanase produced from a potential trichoderma harzianum strain hzn11 was purified to homogeneity by deae-sepharose and sephadex g-100 chromatography with 33.12 fold purification with specific activity of 66.25 u/mg and molecular mass of ~55 kda. the optimum temperature and ph were 60 °c and 5.5 retaining 76 and 85 % of activity after 3 h, respectively. it showed stability between ph 4.5-6.0 and temperature between 50-70 °c indicating thermostabi ... | 2016 | 28330171 |
| potential of trichoderma harzianum for control of banana leaf fungal pathogens when applied with a food source and an organic adjuvant. | trichoderma isolates were obtained from diseased leaves and fruit collected from plantations in the main banana production area in northern queensland. phylogenetic analyses identified the trichoderma isolates as t. harzianum and t. virens. the trichoderma spp. were found to be antagonistic against the banana leaf pathogens mycosphaerella musicola, cordana musae, and deightoniella torulosa in vitro. several products used by the banana industry to increase production, including molasses, fishoil ... | 2016 | 28330078 |
| characterization and enzymatic hydrolysis of hydrothermally treated β-1,3-1,6-glucan from aureobasidium pullulans. | the chemical structure of hydrothermally treated β-1,3-1,6-glucan from aureobasidium pullulans was characterized using techniques such as gas chromatography/mass spectrometry (gc/ms) and nuclear magnetic resonance (nmr). the chemical shifts of anomeric carbons observed in the (13)c-nmr spectra suggested the presence of single flexible chains of polysaccharide in the sample. β-1,3-1,6-glucan from a. pullulans became water-soluble, with an average molecular weight of 128,000 da after hydrothermal ... | 2016 | 27804105 |
| effect of seed pelleting with biocontrol agents on growth and colonisation of roots of mungbean by root-infecting fungi. | mungbean (vigna radiata (l.) wilczek) is a leguminous pulse crop that is a major source of proteins, vitamins and minerals. root-infecting fungi produce severe plant diseases like root rot, charcoal rot, damping-off and stem rot. the soil-borne pathogens can be controlled by chemicals, but these chemicals have several negative effects. use of microbial antagonist such as fungi and bacteria is a safe, effective and eco-friendly method for the control of many soil-borne pathogens. biological contr ... | 2016 | 26619828 |
| phosphate dissolving fungi: mechanism and application in alleviation of salt stress in wheat. | the present investigation reveals the solubilization efficiency of tri-calcium phosphate (tcp), udaipur rock phosphate (urp), aluminium phosphate (ap) and ferric phosphate (fp) by aspergillus niger (itcc 6719) and trichoderma harzianum (itcc 6721) as function of carbon concentrations. increasing glucose concentration from 1 to 7% in the growth medium, though improved the phosphorus (p) solubilization significantly but each fungal strain preferred different optimum carbon concentrations for media ... | 2016 | 27825490 |
| design of an enzyme cocktail consisting of different fungal platforms for efficient hydrolysis of sugarcane bagasse: optimization and synergism studies. | lignocellulosic materials represent a very important and promising source of renewable biomass. in order to turn them into fermentable sugars, synergism among the different enzymes that carry out bioconversion of these materials is one of the main factors that should be considered. experimental mixture design was performed to optimize the proportion of enzymes produced by native strains of trichoderma harzianum ioc 3844, penicillium funiculosum atcc 11797, and aspergillus niger atcc 1004, result ... | 2016 | 27254751 |
| winery biomass waste degradation by sequential sonication and mixed fungal enzyme treatments. | to increase the efficiency of winery-derived biomass biodegradation, grape pomace was ultrasonicated for 20min in the presence of 0.25m, 0.5mand1.0mkoh and 1.0mnaoh. this was followed by treatment with a 1:1 (v/v) mix of crude enzyme preparation derived from phanerochaete chrysosporium and trametes versicolor for 18h and a further 18h treatment with a 60:14:4:2 percent ratio combination of enzymes derived from aspergillus niger: penicillium chrysogenum: trichoderma harzianum: p. citrinum, repsec ... | 2016 | 27599392 |
| characterization of the cellulolytic secretome of trichoderma harzianum during growth on sugarcane bagasse and analysis of the activity boosting effects of swollenin. | this study demonstrates the production of an active enzyme cocktail produced by growing trichoderma harzianum on sugarcane bagasse. the component enzymes were identified by lcms-ms. glycosyl hydrolases were the most abundant class of proteins, representing 67% of total secreted protein. other carbohydrate active enzymes involved in cell wall deconstruction included lytic polysaccharide mono-oxygenases (aa9), carbohydrate-binding modules, carbohydrate esterases and swollenin, all present at level ... | 2016 | 26697775 |
| nitrogen metabolism and growth enhancement in tomato plants challenged with trichoderma harzianum expressing the aspergillus nidulans acetamidase amds gene. | trichoderma is a fungal genus that includes species that are currently being used as biological control agents and/or as biofertilizers. in addition to the direct application of trichoderma spp. as biocontrol agents in plant protection, recent studies have focused on the beneficial responses exerted on plants, stimulating the growth, activating the defenses, and/or improving nutrient uptake. the amds gene, encoding an acetamidase of aspergillus, has been used as a selectable marker for the trans ... | 2016 | 27536277 |
| identification, characterization and phylogenetic analysis of antifungal trichoderma from tomato rhizosphere. | the use of trichoderma isolates with efficient antagonistic activity represents a potentially effective and alternative disease management strategy to replace health hazardous chemical control. in this context, twenty isolates were obtained from tomato rhizosphere and evaluated by their antagonistic activity against four fungal pathogens (fusarium oxysporum f. sp. lycopersici, alternaria alternata, colletotrichum gloeosporoides and rhizoctonia solani). the production of extracellular cell wall d ... | 2016 | 27917337 |
| microbe-based technology ameliorates glandular trichomes, secondary metabolites and antioxidants in pelargonium graveolens l'hér. | despite the vast exploration of microbes for plant health, there is a lack of knowledge about the synergistic effects of specific microorganisms in sustainable agriculture, especially in medicinal plants such as pelargonium graveolens l'hér. the aim of this study was to evaluate how synergistic microbes trichoderma harzianum thu, glomus intraradices and bacillus subtilis cim affected crop productivity, secondary metabolites and glandular trichome number in p. graveolens. | 2016 | 26762896 |
| genetic variation and biological control of fusarium graminearum isolated from wheat in assiut-egypt. | fusarium graminearum schwabe causes fusarium head blight (fhb), a devastating disease that leads to extensive yield and quality loss of wheat and other cereal crops. twelve isolates of f. graminearum were collected from naturally infected spikes of wheat from assiut egypt. these isolates were compared using srap. the results indicated distinct genetic groups exist within f. graminearum, and demonstrated that these groups have different biological properties, especially with respect to their path ... | 2016 | 27147934 |
| cumulative role of bioinoculants on growth, antioxidant potential and artemisinin content in artemisia annua l. under organic field conditions. | artemisia annua l. is mostly known for a bioactive metabolite, artemisinin, an effective sesquiterpene lactone used against malaria without any reputed cases of resistance. in this experiment, bioinoculants viz., streptomyces sp. mtn14, bacillus megaterium mtn2rp and trichoderma harzianum thu were applied as growth promoting substances to exploit full genetic potential of crops in terms of growth, yield, nutrient uptake and particularly artemisinin content. further, multi-use of the bioinoculant ... | 2016 | 27565777 |
| parasitic weed management by using strigolactone-degrading fungi. | seed germination is a key phase of the parasitic plant life cycle that is stimulated by the secondary metabolites, mainly strigolactones (sls), secreted by the host roots. interventions during this stage would be particularly suitable for parasitic weed management practices, as blocking these chemical signals would prevent seed germination and thus parasite attack. four fungal strains with different ecological functions were considered for their possible ability to metabolise sls: fusarium oxysp ... | 2016 | 26757233 |
| molecular cloning and characterization of ech46 endochitinase from trichoderma harzianum. | in the present study, endochitinase of t. harzianum isolate-thhp3 induced against mycelium of f. oxysporum was cloned, sequenced and characterized. the complete nucleotide sequence contained an orf of 1293bp corresponding to 430 amino acids with 46kda molecular weight and theoretical pi 5.59. the precursor protein contained 22 amino acids long signal peptide at n terminus. the domain architecture of endochitinase showed low complexity regions, presence of 1w9p domain specific to cyclopentapeptid ... | 2016 | 27456116 |
| crystal structure and biochemical characterization of the recombinant thbgl, a gh1 β-glucosidase overexpressed in trichoderma harzianum under biomass degradation conditions. | the conversion of biomass-derived sugars via enzymatic hydrolysis for biofuel production is a challenge. therefore, the search for microorganisms and key enzymes that increase the efficiency of the saccharification of cellulosic substrates remains an important and high-priority area of study. trichoderma harzianum is an important fungus known for producing high levels of cellulolytic enzymes that can be used for cellulosic ethanol production. in this context, β-glucosidases, which act synergisti ... | 2016 | 27006690 |
| differential response of extracellular proteases of trichoderma harzianum against fungal phytopathogens. | in the present study, production of extracellular proteases by trichoderma harzianum was evaluated based on the relative gene expression and spectrophotometric assay. the fungal isolates were grown in czapek dox broth medium supplemented with deactivated mycelium of plant fungal pathogens such as fusarium oxysporum, colletotrichum capsici, gloeocercospora sorghi, and colletotrichum truncatum. the maximum protease activity was detected after 48 h of incubation against colletotrichum spp. similarl ... | 2016 | 27278806 |
| trichoderma harzianum t-22 induces systemic resistance in tomato infected by cucumber mosaic virus. | understanding the induction of plant defenses against viruses using biocontrol agents is essential for developing new strategies against these pathogens, given the ineffectiveness of chemical treatments. the ability of trichoderma harzianum, strain t-22 (t22) to control cucumber mosaic virus (cmv) in solanum lycopersicum var. cerasiforme plants and the changes in the physiology of tomato treated/infected with t22/cmv were examined. plant growth-promoting effects, photosynthetic performance, reac ... | 2016 | 27777581 |
| trichoderma species occurring on wood with decay symptoms in mountain forests in central europe: genetic and enzymatic characterization. | the aim of this study was to explore the species diversity of trichoderma obtained from samples of wood collected in the forests of the gorce mountains (location a), karkonosze mountains (location b) and tatra mountains (location c) in central europe and to examine the cellulolytic and xylanolytic activity of these species as an expression of their probable role in wood decay processes. the present study has led to the identification of the following species and species complex: trichoderma atro ... | 2016 | 26586561 |
| native fungi as metal remediators: silver myco-accumulation from metal contaminated waste-rock dumps (libiola mine, italy). | metal contamination constitutes a major source of pollution globally. many recent studies emphasized the need to develop cheap and green technologies for the remediation or reclamation of environmental matrices contaminated by heavy metals. in this context, fungi are versatile organisms that can be exploited for bioremediation activities. in our work, we tested silver (ag) bioaccumulation capabilities of three microfungal strains (aspergillus alliaceus thom & church, trichoderma harzianum rifai, ... | 2017 | 28121268 |
| elucidation of biocontrol mechanisms of trichoderma harzianum against different plant fungal pathogens: universal yet host specific response. | in the present study, different transcripts of trichoderma harzianum thhp-3 were evaluated for their response against four fungal pathogens fusarium oxysporum, colletotrichum capsici, colletotrichum truncatum and gloesercospora sorghi using rt-qpcr. the time course study of t. harzianum transcripts related to signal transduction, lytic enzymes, secondary metabolites and various transporters revealed variation in expression against four fungal pathogens. in a broader term, the transcripts were up ... | 2017 | 27856319 |
| evaluating the effects of microwave-assisted hydrodistillation on antifungal and radical scavenging activities of oliveria decumbens and chaerophyllum macropodum essential oils. | in this study, radical scavenging and antifungal activities of chaerophyllum macropodum and oliveria decumbens essential oils (eos) extracted with microwave-assisted hydrodistillation (mahd) were evaluated in comparison with the same eos extracted by conventional hydrodistillation (hd). the final eo yields that were obtained using hd (after 150 min) and mahd (after 45 min) were 1.72 and 1.67% for c. macropodum and 8.10 and 7.91% for o. decumbens, respectively. there were no significant differenc ... | 2017 | 28371590 |
| antibiosis of trichoderma spp strains native to northeastern mexico against the pathogenic fungus macrophomina phaseolina. | sampling of agricultural soils from the mexican northeastern region was performed to detect trichoderma spp., genetically characterize it, and assess its potential use as a biologic control agent against macrophomina phaseolina. m. phaseolina is a phytopathogen that attacks over 500 species of cultivated plants and causes heavy losses in the regional sorghum crop. sampling was performed immediately after sorghum or corn harvest in an area that was approximately 170 km from the mexico-usa border. ... | 2017 | 26691467 |
| determination of lytic enzyme activities of indigenous trichoderma isolates from pakistan. | this study investigated lytic enzyme activities in three indigenous trichoderma strains namely, trichoderma asperellum, trichoderma harzianum and trichoderma sp. native trichoderma strains and a virulent strain of rhizoctonia solani isolated from infected bean plants were also included in the study. enzyme activities were determined by measuring sugar reduction by dinitrosalicylic acid (dns) method using suitable substrates. the antagonists were cultured in minimal salt medium with the following ... | 2017 | 26691463 |
| isolation and expression of two polyketide synthase genes from trichoderma harzianum 88 during mycoparasitism. | metabolites of mycoparasitic fungal species such as trichoderma harzianum 88 have important biological roles. in this study, two new ketoacyl synthase (ks) fragments were isolated from cultured trichoderma harzianum 88 mycelia using degenerate primers and analysed using a phylogenetic tree. the gene fragments were determined to be present as single copies in trichoderma harzianum 88 through southern blot analysis using digoxigenin-labelled ks gene fragments as probes. the complete sequence analy ... | 2017 | 26991299 |
| structural basis of transport function in major facilitator superfamily protein from trichoderma harzianum. | trichothecenes are the sesquiterpenes secreted by trichoderma spp. residing in the rhizosphere. these compounds have been reported to act as plant growth promoters and bio-control agents. the structural knowledge for the transporter proteins of their efflux remained limited. in this study, three-dimensional structure of thmfs1 protein, a trichothecene transporter from trichoderma harzianum, was homology modelled and further molecular dynamics (md) simulations were used to decipher its mechanism. ... | 2017 | 27816530 |
| functions of thga1 gene in trichoderma harzianum based on transcriptome analysis. | trichoderma spp. are important biocontrol filamentous fungi, which are widely used for their adaptability, broad antimicrobial spectrum, and various antagonistic mechanisms. in our previous studies, we cloned thga1 gene encoding gαi protein from trichoderma harzianum th-33. its knockout mutant showed that the growth rate, conidial yield, camp level, antagonistic action, and hydrophobicity decreased. therefore, illumina rna-seq technology (rna-seq) was used to determine transcriptomic differences ... | 2017 | 27672660 |
| addition of surfactants and non-hydrolytic proteins and their influence on enzymatic hydrolysis of pretreated sugarcane bagasse. | poly(ethylene glycol) (peg 4000) and bovine serum albumin (bsa) were investigated with the purpose of evaluating their influence on enzymatic hydrolysis of sugarcane bagasse. effects of these supplements were assayed for different enzymatic cocktails (trichoderma harzianum and penicillium funiculosum) that acted on lignocellulosic material submitted to different pretreatment methods with varying solid (25 and 100 g/l) and protein (7.5 and 20 mg/g cellulose) loadings. the highest levels of glucos ... | 2017 | 27631122 |
| the improvement of competitive saprophytic capabilities of trichoderma species through the use of chemical mutagens. | the antagonistic potential of trichoderma strains was assayed by studying the effect of their culture filtrate on the radial growth of sclerotium rolfsii, the causal agent of chickpea collar rot. trichoderma harzianum-1432 (42.2%) and trichoderma atroviride (40.3%) were found to be strong antagonists. to enhance their antagonistic potential, mutagenesis of these two selected strains was performed. two mutants, th-m1 and t. atroviride m1, were found to be more effective than their parent strains. ... | 2017 | 26887221 |
| microplate-based evaluation of the sugar yield from giant reed, giant miscanthus and switchgrass after mild chemical pre-treatments and hydrolysis with tailored trichoderma enzymatic blends. | giant reed, miscanthus, and switchgrass are considered prominent lignocellulosic feedstocks to obtain fermentable sugars for biofuel production. the bioconversion into sugars requires a delignifying pre-treatment step followed by hydrolysis with cellulase and other accessory enzymes like xylanase, especially in the case of alkali pre-treatments, which retain the hemicellulose fraction. blends richer in accessory enzymes than commercial mix can be obtained growing fungi on feedstock-based substra ... | 2017 | 28386674 |
| integrated control of wood destroying basidiomycetes combining cu-based wood preservatives and trichoderma spp. | the production of new generation of wood preservatives (without addition of a co-biocide) in combination with an exchange of wood poles on identical sites with high fungal inoculum, has resulted in an increase of premature failures of wood utility poles in the last decades. wood destroying basidiomycetes inhabiting sites where poles have been installed, have developed resistance against wood preservatives. the objective of the in vitro studies was to identify a trichoderma spp. with a highly ant ... | 2017 | 28379978 |
| fungal biomass protein production from trichoderma harzianum using rice polishing. | industrially important enzymes and microbial biomass proteins have been produced from fungi for more than 50 years. high levels of crude protein as much as 45% are present in fungal biomass with balanced essential amino acids. the aim of this study was to access the potential of trichoderma harzianum to produce fungal biomass protein from rice polishings. maximum biomass yield was obtained at 5% (w/v) rice polishings after 72 h of incubation at 28°c at ph 4. carbon and nitrogen ratio of 20 : 1 g ... | 2017 | 28367444 |
| draft genome sequence of the root-colonizing fungus trichoderma harzianum b97. | trichoderma harzianum is one of the most beneficial microorganisms applied on diverse crops against biotic and abiotic stresses and acts also as a plant growth-promoting fungus. here, we report the genome of t. harzianum b97, originating from a french agricultural soil and used as a biofertilizer that can tolerate abiotic stresses. | 2017 | 28360171 |
| applying taguchi design and large-scale strategy for mycosynthesis of nano-silver from endophytic trichoderma harzianum sya.f4 and its application against phytopathogens. | development of reliable and low-cost requirement for large-scale eco-friendly biogenic synthesis of metallic nanoparticles is an important step for industrial applications of bionanotechnology. in the present study, the mycosynthesis of spherical nano-ag (12.7 ± 0.8 nm) from extracellular filtrate of local endophytic t. harzianum sya.f4 strain which have interested mixed bioactive metabolites (alkaloids, flavonoids, tannins, phenols, nitrate reductase (320 nmol/hr/ml), carbohydrate (25 μg/μl) an ... | 2017 | 28349997 |
| relationship of microbial communities and suppressiveness of trichoderma fortified composts for pepper seedlings infected by phytophthora nicotianae. | the understanding of the dynamic of soil-borne diseases is related to the microbial composition of the rhizosphere which is the key to progress in the field of biological control. trichoderma spp. is commonly used as a biological control agent. the use of next generation sequencing approaches and quantitative pcr are two successful approaches to assess the effect of using compost as substrate fortified with two trichoderma strains (trichoderma harzianum or trichoderma asperellum) on bacterial an ... | 2017 | 28346470 |
| the combination of trichoderma harzianum and chemical fertilization leads to the deregulation of phytohormone networking, preventing the adaptive responses of tomato plants to salt stress. | plants have evolved effective mechanisms to avoid or reduce the potential damage caused by abiotic stresses. in addition to biocontrol abilities, trichoderma genus fungi promote growth and alleviate the adverse effects caused by saline stress in plants. morphological, physiological, and molecular changes were analyzed in salt-stressed tomato plants grown under greenhouse conditions in order to investigate the effects of chemical and biological fertilizations. the application of trichoderma harzi ... | 2017 | 28303151 |
| biogenic silver nanoparticles based on trichoderma harzianum: synthesis, characterization, toxicity evaluation and biological activity. | white mold is an agricultural disease caused by the fungus sclerotinia sclerotiorum, which affects important crops. there are different ways of controlling this organism, but none provides inhibition of its resistance structures (sclerotia). nanotechnology offers promising applications in agricultural area. here, silver nanoparticles were biogenically synthesized using the fungus trichoderma harzianum and characterized. cytotoxicity and genotoxicity were evaluated, and the nanoparticles were ini ... | 2017 | 28300141 |
| fungi-based treatment of brewery wastewater-biomass production and nutrient reduction. | the beer-brewing process produces high amounts of nutrient-rich wastewater, and the increasing number of microbreweries worldwide has created a need for innovative solutions to deal with this waste. in the present study, fungal biomass production and the removal of organic carbon, phosphorus and nitrogen from synthetic brewery wastewater were studied. different filamentous fungi with a record of safe use were screened for growth, and trametes versicolor, pleurotus ostreatus and trichoderma harzi ... | 2017 | 28213731 |
| the relation between xyr1 overexpression in trichoderma harzianum and sugarcane bagasse saccharification performance. | this work investigates the influence of the positive regulator xyr1 of trichoderma harzianum on the production of cellulolytic enzymes, using sugarcane bagasse as carbon source. constitutive expression of xyr1 was achieved under the control of the strong trichoderma reesei pki1 promoter. five clones with xyr1 overexpression achieved higher xyr1 expression and greater enzymatic productivity when cultivated under submerged fermentation, hence validating the genetic construction for t. harzianum. c ... | 2017 | 28192217 |
| assessment of ni accumulation capability by fungi for a possible approach to remove metals from soils and waters. | abandoned industrial sites and mines may constitute possible hazards for surrounding environment due to the presence of toxic compounds that may contaminate soils and waters. the possibility to remove metal contaminants, specifically nickel (ni), by means of fungi was presented exploiting a set of fungal strains isolated from a ligurian dismissed mine. the achieved results demonstrate the high ni(ii) tolerance, up to 500 mg ni l(-1), and removal capability of a trichoderma harzianum strain. this ... | 2017 | 28121266 |
| detecting the hormonal pathways in oilseed rape behind induced systemic resistance by trichoderma harzianum th12 to sclerotinia sclerotiorum. | plants have the ability to resist pathogen attack after infection or treatment with biotic and abiotic elicitors. in oilseed rape plant brassica napus aacc and in the artificially synthesized raphanus alboglabra rrcc, the root-colonizing trichoderma harzianum th12 fungus triggers induced systemic resistance (isr), and its culture filtrate (cf) triggers a systemic acquired resistance (sar) response against infection by the sclerotinia sclerotiorum. salicylic acid (sa) and jasmonic acid/ethylene ( ... | 2017 | 28045929 |
| induction of sa-signaling pathway and ethylene biosynthesis in trichoderma harzianum-treated tomato plants after infection of the root-knot nematode meloidogyne incognita. | salicylic acid-signaling pathway and ethylene biosynthesis were induced in tomato treated with trichoderma harzianum when infected by root-knot nematodes and limited the infection by activation of sar and ethylene production. soil pre-treatment with trichoderma harzianum (th) strains item 908 (t908) and t908-5 decreased susceptibility of tomato to meloidogyne incognita, as assessed by restriction in nematode reproduction and development. the effect of t. harzianum treatments on plant defense was ... | 2017 | 28239746 |
| trichoderma harzianum containing 1-aminocyclopropane-1-carboxylate deaminase and chitinase improved growth and diminished adverse effect caused by fusarium oxysporum in soybean. | an isolate, named trichoderma harzianum t-soybean, showed growth-promoting for soybean seedlings and induced resistance to fusarium oxysporum under greenhouse. compared to control soybean seedlings, fresh weight, dry weight, lateral root number, chlorophyll content, root activity and soluble protein of plants pretreated with t-soybean increased, but initial pod height reduced. furthermore, we found that t-soybean inhibited the growth of f. oxysporum by parasitic function. in addition, plate test ... | 2017 | 28135657 |
| effect of paecilomyces lilacinus, trichoderma harzianum and trichoderma virens fungal extracts on the hatchability of ancylostoma eggs. | ancylostoma species have demanded attention due to their zoonotic potential. the use of anthelmintics is the usual method to prevent environmental contamination by ancylostoma eggs and larvae. nematophagous fungi have been widely used in their biological control due to the fungus ability to capture and digest free nematode forms. | 2017 | 27810261 |
| identification of an acidic endo-polygalacturonase from penicillium oxalicum cz1028 and its broad use in major tropical and subtropical fruit juices production. | endo-polygalacturonases play an important role on depectinization in fruit juices industry. a putative endo-polygalacturonase gene poxaenpg28a was cloned from penicillium oxalicum cz1028. poxaenpg28a consisted of a putative signal peptide and a catalytic domain belonging to glycoside hydrolase family 28, and it shared 72% identity with that of a functionally characterized endo-polygalacturonase from trichoderma harzianum. gene poxaenpg28a was successfully expressed in pichia pastoris with a high ... | 2017 | 28254341 |
| systematics of the trichoderma harzianum species complex and the re-identification of commercial biocontrol strains. | trichoderma harzianum is known as a cosmopolitan, ubiquitous species associated with a wide variety of substrates. it is possibly the most commonly used name in agricultural applications involving trichoderma, including biological control of plant diseases. while various studies have suggested that t. harzianum is a species complex, only a few cryptic species are named. in the present study the taxonomy of the t. harzianum species complex is revised to include at least 14 species. previously nam ... | 2017 | 25661720 |
| enhanced production of curdlan by coupled fermentation system of agrobacterium sp. atcc 31749 and trichoderma harzianum gim 3.442. | a coupled fermentation system of agrobacterium sp. atcc 31749 and trichoderma harzianum gim 3.442 (at-cfs) with wheat bran as the optimal nitrogen source was established for producing low-molecular-weight curdlan with high production, which can potentially reduce the cost of low-molecular-weight curdlan biosynthesis. the initial inoculate ratio, ph and the fermentation time were optimized. compared with the curdlan from the single fermentation system of agrobacterium sp. atcc 31749 (a-sfs), the ... | 2017 | 27987884 |
| evaluation of dye compounds' decolorization capacity of selected h. haematococca and t. harzianum strains by principal component analysis (pca). | the selected strains of microscopic fungi, haematonectria haematococca (bwiii43, k37) and trichoderma harzianum (bsiii33), decolorized the following monoathraquinone dyes with different efficiency: 0.03 % alizarin blue black b, 0.01 % carminic acid, 0.01 % poly r-478, and 0.2 % post-industrial lignin. the most effective was the removal of 0.03 % alizarin blue black b (50-60 %) and 0.01 % carminic acid (55-85 %). the principal component analysis (pca) method was applied to determine the main enzy ... | 2017 | 26160991 |