Publications

structure of a lectin with antitumoral properties in king bolete (boletus edulis) mushrooms.	a novel lectin has been isolated from the fruiting bodies of the common edible mushroom boletus edulis (king bolete, penny bun, porcino or cep) by affinity chromatography on a chitin column. we propose for the lectin the name bel (b. edulis lectin). bel inhibits selectively the proliferation of several malignant cell lines and binds the neoplastic cell-specific t-antigen disaccharide, galß1-3galnac. the lectin was structurally characterized: the molecule is a homotetramer and the 142-amino acid ...	2011	21303815
effect of ultrahigh hydrostatic pressure on the activity and structure of mushroom (agaricus bisporus) polyphenoloxidase.	high hydrostatic pressure (hhp, treatment pressure ≤700 mpa) is approved to be the most successful commercial nonthermal processing due to its minimal modifications in nutritional and sensory quality. however, for some pressure stable enzymes such as ppo, this unique technology can hardly inactivate them at treatment pressure below of 700 mpa. this study investigated the effects of ultrahigh hydrostatic pressure (uhhp, treatment pressure >700 mpa) on the activity of agaricus bisporus mushroom po ...	2011	22136649
Study of enzymatic properties of phenol oxidase from nitrogen-fixing Azotobacter chroococcum.	ABSTRACT: Azotobacter chroococcum is a widespread free-living soil bacterium within the genus of Azotobacter known for assimilation of atmospheric nitrogen and subsequent conversion into nitrogenous compounds, which henceforth enrich the nitrogen content of soils. A. chroococcum SBUG 1484, isolated from composted earth, exhibits phenol oxidase (PO) activity when growing under nitrogen-fixing conditions. In the present study we provide incipient analysis of the crude PO activity expressed by A. c ...	2011	21906365
mixed-type inhibition of tyrosinase from agaricus bisporus by terephthalic acid: computational simulations and kinetics.	tyrosinase inhibition studies are needed due to the agricultural and medicinal applications. for probing effective inhibitors of tyrosinase, a combination of computational prediction and enzymatic assay via kinetics were important. we predicted the 3d structure of tyrosinase from agaricus bisporus, used a docking algorithm to simulate binding between tyrosinase and terephthalic acid (tpa) and studied the reversible inhibition of tyrosinase by tpa. simulation was successful (binding energies for ...	2011	21562848
crystal structure of agaricus bisporus mushroom tyrosinase: identity of the tetramer subunits and interaction with tropolone.	tyrosinase catalyzes the conversion of phenolic compounds into their quinone derivatives, which are precursors for the formation of melanin, a ubiquitous pigment in living organisms. because of its importance for browning reactions in the food industry, the tyrosinase from the mushroom agaricus bisporus has been investigated in depth. in previous studies the tyrosinase enzyme complex was shown to be a h(2)l(2) tetramer, but no clues were obtained of the identities of the subunits, their mode of ...	2011	21598903
sulfite determination by an inhibitor biosensor-based mushroom (agaricus bisporus) tissue homogenate.	abstract: the aim of the study presented here is to develop a biosensor based on mushroom (agaricus bisporus) tissue homogenate for sensitive and economical determination of sulfite in foods. the working principle of the biosensor is based on an inhibition effect of sulfite on polyphenol oxidases in mushroom. mushroom tissue homogenate was immobilized by gelatin and glutaraldehyde on a clark-type oxygen electrode. some optimization studies related to the bioactive layer components and working co ...	2011	21714707
quantitative genetics to dissect the fungal-fungal interaction between lecanicillium verticillium and the white button mushroom agaricus bisporus.	lecanicillium fungicola (formerly verticillium fungicola) is responsible for dry bubble disease in the white button mushroom agaricus bisporus. selection for resistance to this pathogen raises an important challenge for mushroom breeders. we have investigated the inheritance of resistance to dry bubble under artificial inoculation in three independent experiments, using a progeny of 89 hybrids derived from an intervarietal a. bisporus var. bisporus×a. bisporus var. burnettii cross. overall, phen ...	2011	21530924
inhibitory effect of a glycoprotein isolated from golden oyster mushroom (pleurotus citrinopileatus) on the lipopolysaccharide-induced inflammatory reaction in raw 264.7 macrophage.	mushrooms have become an important source of natural antitumor, antiviral, anti-bacterial, immunomodulatory, and anti-inflammatioy agents. golden oyster mushroom, pleurotus citrinopileatus, is a common mushroom in oriental countries for human consumption. the present study was to investigate the anti-inflammatory reaction and immunomodulatory of the bioactive non-lectin glycoprotein (pcp-3a) isolated from the fresh fruiting body of this mushroom. western blot analysis on lps-induced inos, cox-2, ...	2011	21627087
vitamin d and sterol composition of 10 types of mushrooms from retail suppliers in the united states.	vitamin d(2) (ergocalciferol) and sterols were analyzed in mushrooms sampled nationwide in the united states to update the usda nutrient database for standard reference. vitamin d(2) was assayed using hplc with [(3)h]-vitamin d(3) internal standard and sterols by gc-fid mass spectrometric (ms) confirmation. vitamin d(2) was low (0.1-0.3 ╬╝g/100 g) in agaricus bisporus (white button, crimini, portabella) and enoki, moderate in shiitake and oyster (0.4-0.7 ╬╝g/100 g), and high in morel, chanterell ...	2011	21663327
nutritional characteristics of proteins from the volva and pileus in cultivated mushroom dictyophora rubrovolvata.	dictyophora rubrovolvata is one of the commercially important mushroom species in china. however, its volva and pileus account for about 65% of the whole mushroom (by fresh weight), and most of them are dumped without utilization. to effectively utilize the waste, the present study determined their proximate composition and investigated the nutritional characteristics of their proteins via the analysis of amino acid composition and protein fractionation. the results showed that the volva and pil ...	2011	21284496
structural basis for ligand recognition in a mushroom lectin: solvent structure as specificity predictor.	lectins are able to recognize specific carbohydrate structures through their carbohydrate recognition domain (crd). the lectin from the mushroom agaricus bisporus (abl) has the remarkable ability of selectively recognizing the tf-antigen, composed of galß1-3galnac, ser/thr linked to proteins, specifically exposed in neoplastic tissues. strikingly, the recently solved crystal structure of tetrameric abl in the presence of tf-antigen and other carbohydrates showed that each monomer has two crds, e ...	2011	21453906
Antioxidant properties of extracts of wild medicinal mushroom species from Croatia.	Antioxidant activity and total phenol (TP) content of methanol and water extracts of three wild Croatian mushroom species Auricularia auricula-judae (Bull.) Quél., Sarcoscypha austriaca (Sacc.) Boud., and Strobilurus esculentus (Wulfen) Singer were determined and compared with the values obtained for extracts of four cultivated mushrooms Agaricus bisporus (J.E. Lange) Imbach (brown and white strains), Pleurotus ostreatus (Jacq.) P. Kumm., and Lentinus edodes (Berk.) Singer. Spectrophotometric de ...	2011	22135877
chemical composition and nutrition value of dried cultivated culinary-medicinal mushrooms from turkey.	dietary fiber, raw fiber, fructose, glucose, sucrose, maltose, vitamin a (retinol), b1 (thiamine), b2 (riboflavin), b6 (pyridoxine), and niacin contents of dried cultivated mushroom species agaricus bisporus (white and brown), lentinus edodes, and pleurotus ostreatus were determined and evaluated for nutrient and chemical composition. assays of dried mushroom samples were carried out after the drying process. dried shiitake samples showed the highest dietary fiber and raw fiber content (23.23 +/ ...	2011	22164765
mutual influence of soil basidiomycetes and white mustard plants on their enzymatic and catabolic activities.	liquid and volatile emanations in interactions of soil basidiomycetes with herbs affect fungal oxidoreductases and stress-related plant peroxidases (po). in this study, gnotobiotic co-cultures between 6 non-pathogenic saprobes and 2 ectomycorrhizal basidiomycetes with the non-host plant white mustard were established on glucose-salt medium with the respective controls. determined were oxidoreductase activities for culture fluids and plant tissues at initial fungal idiophase and degradation rates ...	2011	20806256
Chemical composition and nutritional value of the most widely appreciated cultivated mushrooms: An inter-species comparative study.	Herein, it was reported and compared the chemical composition and nutritional value of the most consumed species as fresh cultivated mushrooms: Agaricus bisporus (white and brown mushrooms), Pleurotus ostreatus (oyster mushroom), Pleurotus eryngii (King oyster mushroom), Lentinula edodes (Shiitake) and Flammulina velutipes (Golden needle mushroom). Shiitake revealed the highest levels of macronutrients, unless proteins, as also the highest sugars, tocopherols and PUFA levels, and the lowest SFA ...	2011	22056333
evaluation of indigenous potent mushroom growth promoting bacteria (mgpb) on agaricus bisporus production.	mushrooms such as agaricus bisporus, are cultivated for food worldwide. fruit body initiation in agaricus bisporus is a phase change from the vegetative to the reproductive stage which depends on the presence of a casing layer with particular physical, chemical and microbiological properties. the phase change is achieved practically by environmental manipulation and the presence of naturally occurring bacteria such as pseuodomonas putida. in this study, 274 individual bacterial isolates were col ...	2011	22806784
storage and preservation of temperate mushroom cultures, agaricus bisporus and pleurotus florida.	two temperate mushroom cultures namely agaricus bisporus (u-3) and pleurotus florida (pau-5) were evaluated for their physiological (linear growth and biomass production), biochemical (β-1,4 endoglucanase production) and fruiting behaviour after preservation in 10% (v/v) glycerol and storage at room temperature (25-35°c), -20°c and -196°c for 6 months with the objective of establishing the recovery/changes in these fungi after storage. studies indicated that the viability and recovery of a. bisp ...	2011	22654171
the cultivation of the mushroom agaricus bisporus (champignon): micro-organisms and preservability.	cultivation of agaricus bisporus mushrooms requires the use of substrates that are potentially dangerous from the microbiological point of view, such as chicken and horse manure. microorganisms can pose risks to consumers and workers, and generate lower profits. packaging of fresh mushrooms with impermeable films is used to extend their shelf life but creates anaerobic and humidity conditions that could favour the growth of microorganisms such as listeria monocytogenes and clostridium botulinum. ...	2011	22508614
bioremediation of fungicides by spent mushroom substrate and its associated microflora.	experiments were conducted both under in vitro and in situ conditions to determine the biodegradation potential of button mushroom spent substrate (sms) and its dominating microbes (fungi and bacteria) for carbendazim and mancozeb, the commonly used agricultural fungicides. during 6 days of incubation at 30 ± 2°c under broth culture conditions, highest degradation of carbendazim (17.45%) was recorded with b-1 bacterial isolate, while highest degradation of mancozeb (18.05%) was recorded with tri ...	2011	22282605
extraction optimization and bioactivity of exopolysaccharides from agaricus bisporus.	response surface methodology was used to optimize the extraction parameters of eps produced by agaricus bisporus mj-0811 in submerged culture. the optimal levels for ethanol concentration (85%, v/v), ph (8) and precipitation time (22 h) were determined, and eps production was estimated at 2.71 g/l. the actual yield of eps under these conditions was 2.69 g/l. in addition, the antioxidant activity of eps was investigated by measuring its scavenging ability on superoxide radicals and hydroxyl radic ...	2012	23399195
capacity for colonization and degradation of horse manure and wheat-straw-based compost by different strains of agaricus subrufescens during the first two weeks of cultivation.	the potential of agaricus subrufescens strains to colonize and transform horse manure and wheat-straw-based mushroom compost under the physico-chemical conditions typically used for agaricus bisporus was assessed. lignocellulolytic activities, h2o2 production and substrate transformation (assessed via cp/mas nmr of (13)c) for certain a. subrufescens strains were similar or even greater than those obtained for an a. bisporus strain used as control. moreover, the functional diversity of the microb ...	2012	23357087
the first report on mushroom green mould disease in croatia.	green mould disease, caused by trichoderma species, is a severe problem for mushroom growers worldwide, including croatia. trichoderma strains were isolated from green mould-affected agaricus bisporus (button or common mushroom) compost and pleurotus ostreatus (oyster mushroom) substrate samples collected from croatian mushroom farms. the causal agents of green mould disease in the oyster mushroom were t. pleurotum and t. pleuroticola, similar to other countries. at the same time, the pathogen o ...	2012	23334043
direct electrochemistry of dopamine on gold-agaricus bisporus laccase enzyme electrode: characterization and quantitative detection.	direct electrochemistry of a new laccase enzyme immobilized on gold and its application as a biosensor for dopamine (da) are investigated by voltammetry and electrochemical impedance spectroscopy. the sensor demonstrated a redox adsorption behavior with e(0')=+180mv vs. ag/agcl for immobilized agaricus bisporus laccase (lacab) enzyme. the mpa platform was assembled on au with and without utilization of ultrasounds. excellent results were obtained by using the enzyme electrode fabricated based on ...	2012	22137204
Homology models of four Agaricus bisporus tyrosinases.	Partially purified tyrosinase from the white button mushroom Agaricus bisporus is available commercially and is a widely used experimental model for the study of tyrosinase. The structure of an H(2)L(2) tetrameric form of the mushroom enzyme was recently determined by X-ray crystallography. In this structure the two H subunits originate from the PPO3 gene, and the two L subunits are formed by a protein of unknown function with a lectin-like fold. However, the X-ray structures and oligomeric stat ...	2012	22119959
ultrasonic-assisted extraction and in vitro antioxidant activity of polysaccharides from agaricus bisporus.	to optimize ultrasonic-assisted extraction parameters of polysaccharides from agaricus bisporus and evaluate antioxidant activities of a. bisporus polysaccharides.	2012	23320369