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a participatory assessment of ecosystem services and human wellbeing in rural costa rica using photo-voice.human well-being is intricately connected to ecosystem services. a keystone contribution to the ecosystem service literature has been the millennium ecosystem assessment, ma, (ecosystems and human well-being: a framework for assessment, island press, washington, dc; 2003, 2005). much of the work on ecosystem services to date has focused on the assessment and classification of environmental functions. the need for inclusion of community perspectives in ecosystem assessments has been widely recogn ...201222402931
adsorption and kinetic behavior of recombinant multifunctional xylanase in hydrolysis of pineapple stem and bagasse and their hemicellulose for xylo-oligosaccharide production.a recombinant multifunctional xylanase (rmfxase) was utilized to hydrolyze xylan in pineapple stems and bagasse. the feasibility of using rmfxase and the two agricultural by-products for producing xylo-oligosaccharides was demonstrated. adsorption dynamics showed that lignin and cellulose delayed the adsorption equilibrium. the adsorption data fit the langmuir-type adsorption isotherm. the extent of hydrolysis was quantified by measuring the reducing and total sugars in substrate-rmfxase complex ...201222342034
determination of the insecticide imidacloprid in fruit juices using micellar high-performance liquid chromatography.a simple and reliable hplc method was developed to determine imidacloprid, a chloronicotinyl insecticide that has a highly specific affinity to the nicotinic acetylcholine receptor of insects, above its permissible limit of consumption in fruit juices (orange, apple, and a mixture of pineapple and pear). samples were injected directly into a kromasil c18 column, without any pretreatment step, using the micellar mobile phase 0.10 m sodium dodecyl sulfate and 2.5% (v/v) propanol buffered at ph 7 a ...201219916392
quantification of spiroether isomers and herniarin of different parts of matricaria matricarioides and flowers of chamaemelum nobile.a simple hplc-pad-ms method was established to quantitatively analyse two spiroether isomers (cis-en-yn-dicycloether and trans-en-yn-dicycloether) and the main coumarin, herniarin, in chamomile herbs, simultaneously. by using this method, the contents of these three compounds in the flowers of two chamomile species, roman chamomile (chamaemelum nobile) and pineapple weed (matricaria matricarioides), as well as in different parts of pineapple weed, were investigated. it was found that the flowers ...201217260697
determination of ochratoxin a in currants, raisins, sultanas, mixed dried fruit, and dried figs by immunoaffinity column cleanup with liquid chromatography: interlaboratory study.an interlaboratory study was performed on behalf of the food standards agency to evaluate the effectiveness of an affinity column cleanup liquid chromatographic (lc) method for the determination of ochratoxin a in a variety of dried fruit at european regulatory limits. to ensure homogeneity before analysis, laboratory samples are normally slurried with water in the ratio of 5 parts fruit to 4 parts water, and test materials in this form were used in the study. the test portion was extracted with ...201214979698
production and characterization of violacein by locally isolated chromobacterium violaceum grown in agricultural wastes.the present work highlighted the production of violacein by the locally isolated chromobacterium violaceum (genbank accession no. hm132057) in various agricultural waste materials (sugarcane bagasse, solid pineapple waste, molasses, brown sugar), as an alternative to the conventional rich medium. the highest yield for pigment production (0.82 g l⁻¹) was obtained using free cells when grown in 3 g of sugarcane bagasse supplemented with 10% (v/v) of l-tryptophan. a much lower yield (0.15 g l⁻¹) wa ...201222278051
production of cellulases from aspergillus niger ns-2 in solid state fermentation on agricultural and kitchen waste residues.various agricultural and kitchen waste residues were assessed for their ability to support the production of a complete cellulase system by aspergillus niger ns-2 in solid state fermentation. untreated as well as acid and base-pretreated substrates including corn cobs, carrot peelings, composite, grass, leaves, orange peelings, pineapple peelings, potato peelings, rice husk, sugarcane bagasse, saw dust, wheat bran, wheat straw, simply moistened with water, were found to be well suited for the or ...201222503148
biodegradation of selected nigerian fruit peels by the use of a non-pathogenic rhizobium species cwp g34b.this study was carried out to determine the ability of rhizobium species cwp g34b to degrade the peels of selected nigerian fruits. the potential of the bacterium to digest some carbon sources (lactose, maltose, sucrose and mannitol) and peels of some nigerian fruits (pineapple, orange, plantain, banana, pawpaw and mango fruits) was investigated by growing the organism on the substances separately after which dnsa reagent method was used to quantify glucose released into the medium. the results ...201223166567
bacterial reduction of cr(vi) at technical scale--the malaysian experience.the bacterial reduction of cr(vi) from industrial wastewater was evaluated using a 2.0-m(3) bioreactor. liquid pineapple waste was used as a nutrient for the biofilm community formed inside the bioreactor. the use of rubber wood sawdust as packing material was able to immobilize more than 10(6) cfu ml(-1) of acinetobacter haemolyticus cells after 3 days of contact time. complete reduction of 15-240 mg l(-1) of cr(vi) was achieved even after 3 months of bioreactor operation. cr(vi) was not detect ...201222350941
inhibition or enhancement by 4 pacific island food plants against cancers induced by 2 amino-3-methylimidazo[4,5-f]quinoline in male fischer 344 rats.a 1-yr carcinogenicity bioassay was conducted in rats fed 2-amino-3-methylimidazo[4,5-f]quinoline (iq), simultaneously with ain-76/ high-fat (hf) diet alone, or with 10% starch replaced with kumara, pineapple, coconut, or taro, prepared as for a human diet. all of the non-iq treated control, kumara, pineapple, or taro but not coconut-fed rats survived to 1 yr. none of the iq-fed animals survived to 1 yr and although there were minor survival time differences among the groups, none was statistica ...201222211787
evaluation of the odour of aminoleban(®) en, taste-masked with flavoured powders, by human and electronic noses.aminoleban® en is a bitter total enteral nutrient product with an unpleasant odour. the purpose of this study was to evaluate the odour of aminoleban® en, taste-masked with various flavoured powders, by human testing and by using an electronic nose system (alpha m.o.s.).201223488778
color and odor of artificial fruit used to signal potential dispersers in the atlantic forest in brazil.fruit color and odor are the main features regulating the rate of fruit predation and dispersal. the aim of this study was to analyze the effect of odor and color on fruit predators and dispersers. the present study was conducted in a 30ha area of secondary forest in southeastern atlantic brazil. this area was divided into two transects, in which four points were marked with a 30m distance from each other. each sampling point contained a total of 30 artificial fruit which belong to six different ...201223894956
modulation of pulmonary metastasis (lewis lung carcinoma) by bromelain, an extract of the pineapple stem (ananas comosus) 20123378194
identification and validation of reference genes to study the gene expression in gluconacetobacter diazotrophicus grown in different carbon sources using rt-qpcr.gluconacetobacter diazotrophicus strain pal5 is a nitrogen-fixing endophytic bacterium originally isolated from sugarcane and later on was found to colonize other plants such as rice, elephant grass, sweet potato, coffee, and pineapple. currently, g. diazotrophicus has been considered a plant growth-promoting bacterium due to its characteristics of biological nitrogen fixation, phytohormone secretion, solubilization of mineral nutrients and antagonism to phytopathogens. reverse transcription fol ...201222814372
ethnopharmacological survey of medicinal plants used by indigenous and tribal people in rangamati, bangladesh.there is very limited information regarding plants used by traditional healers in rangamati, bangladesh, for treating general ailments. current study provides significant ethnopharmacological information, both qualitative and quantitative on medical plants in rangamati.201223064284
resonance assignments and secondary structure of a phytocystatin from ananas comosus.a cdna encoding a cysteine protease inhibitor, cystatin was cloned from pineapple (ananas comosus l.) stem. this clone was constructed into an expression vector and expressed in e. coli and purified to homogeneous. the recombinant pineapple cystatins (accys) showed effectively inhibitory activity toward cysteine proteases including papain, bromelain, and cathepsin b. in order to unravel its inhibitory action from structural point of view, multidimensional heteronuclear nmr techniques were used t ...201221814766
bromelain, a cysteine protease from pineapple (ananas comosus) stem, is an inhibitor of fungal plant pathogens.this study aimed to evaluate the effect of bromelain, a cysteine protease isolated from pineapple (ananas comosus), on growth of several agronomically important fungal pathogens.201222537505
effect of acid fruit juices combined with electric or sonic toothbrushing on root dentin permeability--an in vitro study.the purpose of this in vitro study was to quantify the alterations on human root dentin permeability after exposure to different acid fruit juices and to evaluate the effect of toothbrushing with electric or sonic toothbrush after acid exposure. the root dentin of 50 extracted third molars was exposed with a high speed bur. crowns were sectioned above the cementoenamel junction and root fragments were used to prepare dentin specimens. specimens were randomly assigned to 5 groups according to the ...201223338258
study of the antifungal potential of novel cellulose/copper composites as absorbent materials for fruit juices.cellulose/copper composites with antifungal properties have been synthesized by physical/chemical methods. physical treatments by heat or by a combination of heat and uv radiation provided composites with metallic copper and excellent interfacial adhesion; in contrast, chemical reduction with borohydride generated small although partially aggregated copper oxide nanoparticles. copper micro/nano-particles and copper ions (cu(2+)) were released from the cellulose matrix at an adequate rate to achi ...201222835229
a highly sensitive single-tube nested pcr assay for the detection of pineapple mealybug wilt associated virus-2 (pmwav-2).an assay was developed for the detection of pineapple mealybug wilt associated virus-2 (pmwav-2), an important factor in the etiology of mealybug wilt of pineapple. the assay combines reverse transcription of rna isolated from pineapple with a specific and very sensitive, single, closed-tube nested polymerase chain reaction (pcr) to amplify a segment of the coat protein gene of the pmwav-2. the outer primers were designed to anneal at higher temperatures than the nested primers to prevent primer ...201222484612
stem bromelain-induced macrophage apoptosis and activation curtail mycobacterium tuberculosis persistence.mycobacterium tuberculosis, the causative agent of tuberculosis, has a remarkable ability to usurp its host's innate immune response, killing millions of infected people annually. one approach to manage infection is prevention through the use of natural agents. in this regard, stem bromelain (sbm), a pharmacologically active member of the sulfhydryl proteolytic enzyme family, obtained from ananas comosus and possessing a remarkable ability to induce the innate and acquired immune systems, is imp ...201222615313
mycotoxins biosynthesized by plant-derived fusarium isolates.there is little information on secondary metabolites produced by fusaria infecting crop plants other than cereals. many members of fusarium genus have the ability to colonise perennial crops with only scarce infection or disease symptoms or with no symptoms at all while still being detectable. even in case of such asymptomatic infection, significant mycotoxin contamination of the plant tissues is possible. the aim of this study was to characterise the spectrum of fusarium species isolates obtain ...201223334038
[vitamin c in fruits and vegetables].strong opinion about reducing vitamin c content in traditional cultivars of fruits and vegetables as a result of intensive farming practices, on the one hand, and depletion of soil, waste of fertilizers, on the other hand, takes place. the aim of the study was to assess changes in vitamin c content in fresh vegetables, fruits and berries from the 40s of last century to the present. available national and foreign data from official tables of the chemical composition tables published in different ...201324006751
factors significantly increasing or inhibiting early stages of malignant melanoma (m.m.) and non-invasive evaluation of new treatment by ingestion and external application of optimal doses of the most effective anti-m.m. substances: haritaki, cilantro, vitamin d3, nori, epa with dha, & application of special (+) solar energy stored paper, which reduced the m.m. active area & asbestos rapidly.sterilizing the pre-cancer skin of malignant melanoma (m.m.) with 70% isopropyl alcohol intensified malignancy & the malignant response extended to surrounding normal looking skin, while sterilizing with 80% (vodka) or 12% (plum wine) ethyl alcohol completely inhibited m.m. in the area (both effects lasted for about 90 minutes initially). burnt food (bread, vegetables, meat, and fish), a variety of smoked & non-smoked fish-skin, many animal's skin, pepper, vitamin c over 75 mg, mango, pineapple, ...201323724697
optimized butyl butyrate synthesis catalyzed by thermomyces lanuginosus lipase.butyl butyrate is an ester present in pineapple flavor, which is very important for the food and beverages industries. in this work, the optimization of the reaction of butyl butyrate synthesis catalyzed by the immobilized lipase lipozyme tl-im was performed. n-hexane was selected as the most appropriate solvent. other reaction parameters such as temperature, substrate molar ratio, biocatalyst content and added water, and their responses measured as yield, were evaluated using a fractional facto ...201323946156
transgenic sweet orange plants expressing a dermaseptin coding sequence show reduced symptoms of citrus canker disease.citrus canker provoked by xanthomonas axonopodis pv. citri is a bacterial disease causing severe losses in all citrus-producing areas around the world. xanthomonas infection is considered as an endemic disease in northeast and northwest argentina, affecting as much as 10% of commercial citrus plantations. there is not known natural resistance neither in orange varieties nor in rootstocks used for grafting of commercial cultivars. to introduce resistance to this disease, plants of pineapple sweet ...201323896218
the use of near infrared spectroscopy and multivariate techniques to differentiate escherichia coli and salmonella enteritidis inoculated into pulp juice.this study shows the application and usefulness of near infrared (nir) transflectance spectra measurements in the identification and classification of escherichia coli and salmonella enteritidis from commercial fruit pulp (pineapple). principal component analysis (pca), soft independent modeling of class analogy (simca) analysis and partial least-squares discriminant analysis (pls-da) were used in the analysis. it was not possible to obtain total separation between the samples using pca and simc ...201323470963
phylogeny and classification of dickeya based on multilocus sequence analysis.bacterial heart rot of pineapple reported in hawaii in 2003 and reoccurring in 2006 was caused by an undetermined species of dickeya. classification of the bacterial strains isolated from infected pineapple to one of the recognized dickeya species and their phylogenetic relationships with dickeya were determined by a multilocus sequence analysis (mlsa), based on the partial gene sequences of dnaa, dnaj, dnax, gyrb and recn. individual and concatenated gene phylogenies revealed that the strains f ...201324003072
in vitro and in vivo evaluation of the activity of pineapple (ananas comosus) on haemonchus contortus in santa inês sheep.the development of resistance to anthelmintics has prompted research into alternative methods of controlling intestinal nematodes in ruminants. this study aimed to assess the activity of ananas comosus on haemonchus contortus in santa inês sheep. the aqueous extract of pineapple skin (aeps), bromelain from pineapple stems (b4882) and residue from pineapple processing was evaluated in in vitro and in vivo tests. the enzymatic activity of substances was analyzed by the azocasein method. the egg ha ...201323688638
in vitro anthelmintic activity of crude extracts of selected medicinal plants against haemonchus contortus from sheep.ethanol extracts of 25 plant species were screened for anthelmintic effects against haemonchus contortus. ethanol extracts of each plant were used at various concentrations (10, 20 and 30%) to treat 10-day faecal cultures, incubated at 27°c with control cultures which were treated with ethanol for 48 h. five plants with high efficacies (ananas comosus, aloe ferox, allium sativum, lespedeza cuneata and warburgia salutaris) were selected from the first screening for further investigation using eth ...201322449714
bromelain inhibits allergic sensitization and murine asthma via modulation of dendritic cells.the incidence of atopic conditions has increased in industrialized countries. persisting symptoms and concern for drug side-effects lead patients toward adjunctive treatments such as phytotherapy. previously, we have shown that bromelain (sbr), a mixture of cysteine proteases from pineapple, ananas comosus, inhibits ovalbumin (ova)-induced murine model of allergic airway disease (aad). however, sbr's effect on development of aad when treatment is administered throughout ova-alum sensitization wa ...201324381635
analysis of mineral and heavy metal content of some commercial fruit juices by inductively coupled plasma mass spectrometry.the presence of potentially toxic elements and compounds in foodstuffs is of intense public interest and thus requires rapid and accurate methods to determine the levels of these contaminants. inductively coupled plasma mass spectrometry is a powerful tool for the determination of metals and nonmetals in fruit juices. in this study, 21 commercial fruit juices (apple, peach, apricot, orange, kiwi, pear, pineapple, and multifruit) present on romanian market were investigated from the heavy metals ...201324453811
bromelain down-regulates myofibroblast differentiation in an in vitro wound healing assay.bromelain, a pineapple-derived enzyme mixture, is a widely used drug to improve tissue regeneration. clinical and experimental data indicate a better outcome of soft tissue healing under the influence of bromelain. proteolytic, anti-bacterial, anti-inflammatory, and anti-oedematogenic effects account for this improvement on the systemic level. it remains unknown, whether involved tissue cells are directly influenced by bromelain. in order to gain more insight into those mechanisms by which brome ...201323771413
incomplete stomach emptying as a complication of intragastric balloon treatment and a solution suggestion: pineapple juice drinking.during removal of intragastric balloon, there is a great deal of gastric undigested food even after an eight-hour starvation. bromelain, a proteolytic enzyme existing in the pineapple juice seems to be a good choice for the undigested food remnants in the stomach. we aimed to investigate the effect of drinking pineapple juice on dissolving food remnants in patients undergoing endoscopic procedure for removal of intragastric balloon.201324254264
errors and myths in feeding and nutrition: impact on the problems of obesity.the increase in obesity prevalence cannot be explained by a sudden and generalized change in human genome. it is certainly due to the modification of lifestyle habits and especially of the diet, as well as a lack of physical activity and sedentary living. changes in the feeding pattern and the subsequent unbalance in the caloric profile of the diet may have had great importance in the occurrence of obesity. the social pressure in relation to the body image, the desire to have a slim body, and th ...201324010747
identification of transcription factors potentially involved in the juvenile to adult phase transition in citrus.the juvenile to adult transition (jat) in higher plants is required for them to reach reproductive competence. however, it is a poorly understood process in woody plants, where only a few genes have been definitely identified as being involved in this transition. this work aims at increasing our understanding of the mechanisms regulating the jat in citrus.201324052558
physicochemical and antioxidant properties of malaysian honeys produced by apis cerana, apis dorsata and apis mellifera.the aim of the present study was to evaluate the physicochemical and antioxidant properties of malaysian monofloral honey samples-acacia, pineapple and borneo honey-and compare them with tualang honey. acacia and pineapple honey are produced by apis mellifera bees while borneo and tualang honey are produced by apis cerana and apis dorsata bees, respectively.201323433009
reverse zymography alone does not confirm presence of a protease inhibitor.reverse zymography is applied for identification and semi-quantification of protease inhibitors that are of protein in nature. however, a protein that shows band in reverse zymography against a protease used for digestion of the gel need not be an inhibitor; it might be resistant to degradation by the protease. we demonstrate that in reverse zymography, avidin, streptavidin and the leaf extract of catharanthus roseus behave like inhibitors of proteases like papain, ficin, bromelain extracts from ...201323417772
changes in vitamin c, phenolic, and carotenoid profiles throughout in vitro gastrointestinal digestion of a blended fruit juice.the aim of this research was to evaluate the influence of an in vitro gastrointestinal digestion on the stability and bioaccessibility of vitamin c, phenolic compounds, and carotenoids, as well as the antioxidant activity in a blended fruit juice (bfj) containing orange, pineapple, and kiwi. vitamin c and most of the analyzed phenolic compounds were quite stable under gastric conditions (recovery > 75%), whereas carotenoids diminished significantly (to 64%). the concentration of all the evaluate ...201323374081
development of pineapple microsatellite markers and germplasm genetic diversity analysis.two methods were used to develop pineapple microsatellite markers. genomic library-based ssr development: using selectively amplified microsatellite assay, 86 sequences were generated from pineapple genomic library. 91 (96.8%) of the 94 simple sequence repeat (ssr) loci were dinucleotide repeats (39 ac/gt repeats and 52 ga/tc repeats, accounting for 42.9% and 57.1%, resp.), and the other three were mononucleotide repeats. thirty-six pairs of ssr primers were designed; 24 of them generated clear ...201324024187
comparison of biofilm and attachment mechanisms of a phytopathological and clinical isolate of klebsiella pneumoniae subsp. pneumoniae.some bacterial species can colonize humans and plants. it is almost impossible to prevent the contact of clinically pathogenic bacteria with food crops, and if they can persist there, they can reenter the human food chain and cause disease. on the leaf surface, microorganisms are exposed to a number of stress factors. it is unclear how they survive in such different environments. by increasing adhesion to diverse substrates, minimizing environmental differences, and providing protection against ...201324222755
glass capillary gas chromatographic analysis of volatile components of canned philippine pineapple juice. 20133693491
antibacterial activity of selected malaysian honey.antibacterial activity of honey is mainly dependent on a combination of its peroxide activity and non-peroxide components. this study aims to investigate antibacterial activity of five varieties of malaysian honey (three monofloral; acacia, gelam and pineapple, and two polyfloral; kelulut and tualang) against staphylococcus aureus, bacillus cereus, escherichia coli, and pseudomonas aeruginosa.201323758747
sonicated pineapple juice as substrate for l. casei cultivation for probiotic beverage development: process optimisation and product stability.the aim of this study was to evaluate the use of sonicated pineapple juice as substrate for producing a probiotic beverage by lactobacillus casei nrrl b442. maximal microbial viability was found by cultivating l. casei at 31°c and ph 5.8 (optimised conditions). after fermentation, samples of sweetened and non-sweetened juice were stored. after 42 days of storage under refrigeration (4°c), the microbial viability was 6.03 log cfu/ml in the non-sweetened sample and 4.77 log cfu/ml in the sweetened ...201323561104
enhancement of ethylenethiourea recoveries in food analyses by addition of cysteine hydrochloride.the effectiveness of cysteine hydrochloride (cys-hcl) as a preservative of ethylenethiourea (etu) in product matrixes and during analysis was studied. etu recoveries were adversely affected by certain product matrixes when fortified directly into the product. recoveries in 8 selected food items were 0-92% when analyzed 30 min after fortification and 0-51% when analyzed after 24 h. when cys-hcl was added to product prior to fortification, recoveries increased to 71-95% even after frozen storage f ...20137580323
evaluation of crude sesquiterpenoid extract of phellinus fastuosus as a natural preservative.it has been revealed that the use of synthetic food preservatives has variety of various side effects and hence search of safe and effective natural preservative has become a need. with the afore said objective the present study to screen the possibility of using sesquiterpenoid extract of local folk mushroom phellinus fastuosus as food preservative was undertaken. microbial growth inhibition by sesquiterpenoid extract of phellinus was evaluated in the media with natural ingredient such as milk ...201318697727
pineapple bromelain induces autophagy, facilitating apoptotic response in mammary carcinoma cells.bromelain, from pineapple, possesses potent anticancer effects. we investigated autophagic phenomenon in mammary carcinoma cells (estrogen receptor positive and negative) under bromelain treatment and also illustrated the relationship between autophagy and apoptosis in mcf-7 cells. mcf-7 cells exposed to bromelain showed delayed growth inhibitory response and induction of autophagy, identified by monodansylcadaverine localization. it was succeeded by apoptotic cell death, evident by sub-g1 cell ...201320848558
chemical shift assignments of the canecystatin-1 from saccharum officinarum.cystatins are cysteine proteases inhibitors that are widely distributed among insects, mammalians and plants. here we report the complete resonance assignment of canecystatin-1 from saccharum officinarum obtained by heteronuclear multidimensional high-resolution nuclear magnetic resonance spectroscopy. the consensus chemical shift index was calculated and showed the presence of one α-helix (residues 27-43) and three β-strands (residues 48-74, 78-89 and 94-104), a secondary structure pattern that ...201322696137
an analysis of consumer demand for fruits in sri lanka. 1981-2010.micronutrient deficiency has become a serious health concern in many countries and sri lanka is no exception. inclusion of vegetables and fruits, which are rich in micronutrients, in the diet is considered as one of the most cost effective measures to alleviate such deficiencies. the purpose of this paper is to analyze consumer demand for fruits in sri lanka. the specific objectives are to: (i) describe the patterns of fruit consumption across various households groups (ii) document the changes ...201323036284
inhibitory effects of a kunitz-type inhibitor from pithecellobium dumosum (benth) seeds against insect-pests' digestive proteinases.pithecellobium dumosum is a tree belonging to the mimosoideae subfamily that presents various previously characterized kunitz-type inhibitors. the present study provides a novel kunitz-trypsin inhibitor isoform purified from p. dumosum seeds. purification procedure was performed by tca precipitation followed by a trypsin-sepharose chromatography and a further reversed-phase hplc. purified inhibitor (pdki-4) showed enhanced inhibitory activity against bovine trypsin and chymotrypsin. furthermore, ...201323238511
anti-inflammatory properties of fruit juices enriched with pine bark extract in an in vitro model of inflamed human intestinal epithelium: the effect of gastrointestinal digestion.enrichment of fruit juices with pine bark extract (pbe) could be a strategy to compensate for phenolic losses during the gastrointestinal digestion. a coculture system with caco-2 cells and raw 264.7 macrophages was established as an in vitro model of inflamed human intestinal epithelium for evaluating the anti-inflammatory capacity of fruit juices enriched with pbe (0.5 g l(-1)) before and after in vitro digestion. the digestion of both pbe-enriched pineapple and red fruit juice led to signific ...201323220608
synthesis, characterization and adsorptive properties of carbon with iron nanoparticles and iron carbide for the removal of as(v) from water.this manuscript presents the synthesis of carbon modified with iron nanoparticles (cfe) and iron carbide (carfe) from the pyrolyzed crown leaves of pineapple (ananas comosus) treated with iron salts. the materials that were obtained were used for the removal of as(v) from aqueous media. the carbonaceous materials were characterized by scanning electron microscopy (sem), transmission electron microscopy (tem), x-ray diffraction (xrd), x-ray photoelectron spectroscopy (xps) and mossbauer spectrosc ...201323201599
agrobacterium-mediated genetic transformation of pineapple (ananas comosus l., merr.).pineapple (ananas comosus l., merr.) is a commercially important crop, grown in the tropical and subtropical regions. however, the crop is faced with postharvest damage and poor varietal and nutritional improvement. being a vegetatively propagated crop, conventional breeding programs take longer time for genetic improvement, which may not necessarily successfully develop an improved cultivar. hence, the genetic modification of pineapple is an alternative handy approach to improve pineapple. we h ...201323179718
relevance of the mention of antioxidant properties in yogurt labels: in vitro evaluation and chromatographic analysis.the purpose of the inclusion of fruit (natural additives) in yogurt aims to increase its antioxidant activity and functionality. herein, a comparative study of the antioxidant potential of yogurts with pieces of various fruits was performed, including yogurts with mention of antioxidant properties in the label. free radicals scavenging activity, reducing power and inhibition of lipid peroxidation were evaluated by in vitro assays, as were the contents in antioxidants such as phenolics, flavonoid ...201326787624
pineapple translation factor sui1 and ribosomal protein l36 promoters drive constitutive transgene expression patterns in arabidopsis thaliana.the availability of a variety of promoter sequences is necessary for the genetic engineering of plants, in basic research studies and for the development of transgenic crops. in this study, the promoter and 5' untranslated regions of the evolutionally conserved protein translation factor sui1 gene and ribosomal protein l36 gene were isolated from pineapple and sequenced. each promoter was translationally fused to the gus reporter gene and transformed into the heterologous plant system arabidopsi ...201323263857
dietary intake of melatonin from tropical fruit altered urinary excretion of 6-sulfatoxymelatonin in healthy volunteers.this study assessed the melatonin content of six tropical fruits and examined whether human consumption could contribute to dietary melatonin as measured by 6-sulfatoxymelatonin (amt6-s, a marker of circulating melatonin in the body). melatonin was extracted using methanol and analyzed by high-performance liquid chromatography. in a clinical crossover study, 30 healthy volunteers consumed selected fruits one at a time, with a 1week wash-out period between fruits, until completing all six fruits. ...201323252791
cytotoxic effects of bromelain in human gastrointestinal carcinoma cell lines (mkn45, kato-iii, ht29-5f12, and ht29-5m21).bromelain is a pineapple stem extract with a variety of therapeutic benefits arising from interaction with a number of different biological processes. several preclinical studies and anecdotal clinical observations have reported the anticancer properties of bromelain. in the present study, we investigated the cytotoxic effects of bromelain in four human cancer cell lines of gastrointestinal origin and the mechanisms involved.201323620673
diversity of fusarium species and mycotoxins contaminating pineapple.pineapple (ananas comosus var. comosus) is an important perennial crop in tropical and subtropical areas. it may be infected by various fusarium species, contaminating the plant material with mycotoxins. the aim of this study was to evaluate fusarium species variability among the genotypes isolated from pineapple fruits displaying fungal infection symptoms and to evaluate their mycotoxigenic abilities. forty-four isolates of ten fusarium species were obtained from pineapple fruit samples: f. ana ...201323572446
structural characterization of a glucuronoarabinoxylan from pineapple (ananas comosus (l.) merrill) gum exudate.native polysaccharide from pineapple gum (panp) was obtained following alkaline extraction of gum and fractionation with cetylpyridinium chloride. it was characterized as a glucuronoarabinoxylan using nmr, methylation data, controlled smith degradation, carboxy-reduction, and esi-ms of oligosaccharides produced on mild acid hydrolysis of panp. hspec-malls-ri of carboxy-reduced fraction showed homogeneous profile (mw 1.943×10(5) g/mol). panp was composed of ara, xyl, gal, and glcpa (40:23:7:30 mo ...201323544593
in vitro activity of pineapple extracts (ananas comosus, bromeliaceae) on rhipicephalus (boophilus) microplus (acari: ixodidae).measures to control the cattle tick, rhipicephalus (boophilus) microplus, based only on chemical products are becoming unsustainable, mainly because of the development of resistance. the objective of this study was to test the effect of the aqueous extract of pineapple skin (aeps) and bromelain extracted from the stem (sigma-aldrich®, b4882) on engorged females and larvae of r. (b.) microplus in vitro. these substances were diluted in water and evaluated at eight concentrations. engorged females ...201323541882
the concept of the eudicot shoot apical meristem as it applies to four spiraea (rosaceae), one mentha (lamiaceae) and one euonymus (celastraceae) cultivars based on chimeric analysis.eversporting eudicots were sought to see if they behave like gymnosperms. behaviour of eversporting gymnosperm chimeras indicates a single apical cell is present in sam and it would be of interest to see if eudicot chimeras have the same behaviour.201323482330
anti-inflammatory activity of bromelia hieronymi: comparison with bromelain.some plant proteases (e. g., papain, bromelain, ficin) have been used as anti-inflammatory agents for some years, and especially bromelain is still being used as alternative and/or complementary therapy to glucocorticoids, nonsteroidal antirheumatics, and immunomodulators. bromelain is an extract rich in cysteine endopeptidases obtained from ananas comosus. in this study the anti-inflammatory action of a partially purified extract of bromelia hieronymi fruits, whose main components are cysteine ...201323364884
production of laccase and manganese peroxidase by pleurotus pulmonarius in solid-state cultures and application in dye decolorization.the production of ligninolytic enzymes (laccase and mn-dependent peroxidase) by the white-rot fungus pleurotus pulmonarius (fr.) quélet was studied in solid-state cultures using agricultural and food wastes as substrate. the highest activities of laccase were found in wheat bran (2,860 ± 250 u/l), pineapple peel (2,450 ± 230 u/l), and orange bagasse (2,100 ± 270 u/l) cultures, all of them at an initial moisture level of 85 %. the highest activities of mn peroxidase were obtained in pineapple pee ...201323645502
activation of words with phonological overlap.multiple lexical representations overlapping with the input (cohort neighbors) are temporarily activated in the listener's mental lexicon when speech unfolds in time. activation for cohort neighbors appears to rapidly decline as soon as there is mismatch with the input. however, it is a matter of debate whether or not they are completely excluded from further processing. we recorded behavioral data and event-related brain potentials (erps) in auditory-visual word onset priming during a lexical d ...201324009593
the physiology of ex vitro pineapple (ananas comosus l. merr. var md-2) as cam or c3 is regulated by the environmental conditions: proteomic and transcriptomic profiles.proteomic and transcriptomic profiles of key enzymes were monitored in pineapple plants propagated under c3 and cam-inducing metabolisms to obtain insight into the cam-facultative metabolism and the relationship of cam plants with oxidative stress.201323959598
adding pineapple juice to a polyethylene glycol-based bowel cleansing regime improved the quality of colon cleaning.an unsuccessful colonoscopy procedure is often related to inadequate bowel cleansing. it is difficult for patients to finish the whole 4 liters of polyethylene glycol-electrolyte lavage (peg-el) because of its salty taste and the large quantity. pineapple juice has been shown to be an effective agent in the dissolution of undigested food in the stomach. this study assessed the effectiveness of both 2 and 4 liters of peg-el in precolonoscopic bowel cleansing and the quality of colonoscopic cleani ...201323949576
serving a variety of vegetables and fruit as a snack increased intake in preschool children.although serving a greater variety of food increases intake, this effect has not been well studied as a strategy to encourage consumption of vegetables and fruit in preschool children.201323902783
pectin methylesterase of datura species, purification, and characterization from datura stramonium and its application.pectin methylesterases (pme; ec 3.1.1.11) involved in de-esterification of pectin and have applicability in food, textiles, wines, pulp, and paper industries. in the present study, we compared pme activity of different parts of 3 datura species and found that fruit coat showed maximum pme activity followed by leaf and seed. pme from leaves of d. stramonium (dspme) was purified and characterized. dspme showed optimum activity at 60 °c and ph 9 in the presence of 0.3 m nacl. dspme was stable at 70 ...201323887498
in vitro bacterial fermentation of tropical fruit fibres.tropical fruits such as mango, papaya, pineapple and banana are rich sources of dietary fibre. however, few studies have examined the potential physiological effects of fibre from these tropical fruits. the aim of this study was to characterise the fermentability of dietary fibre found in banana, papaya, pineapple and mango as an estimate of the physiological effects of consuming these fruits. freeze-dried fruit was subjected to in vitro digestion to remove digestible carbohydrates. digestion re ...201323887033
a novel aspartic acid protease gene from pineapple fruit (ananas comosus): cloning, characterization and relation to postharvest chilling stress resistance.a full-length cdna encoding a putative aspartic acid protease (acap1) was isolated for the first time from the flesh of pineapple (ananas comosus) fruit. the deduced sequence of acap1 showed all the common features of a typical plant aspartic protease phytepsin precursor. analysis of acap1 gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development revealed opposite trends. the resistant variety showed an up-regulation o ...201323838125
deconstructing a fruit serving: comparing the antioxidant density of select whole fruit and 100% fruit juices.research suggests phytonutrients, specifically phenolic compounds, within fruit may be responsible for the putatively positive antioxidant benefits derived from fruit. given the prominence of fruit juice in the american diet, the purpose of this research was to assess the antioxidant density of fresh fruit and 100% fruit juice for five commonly consumed fruits and juices and to compare the adequacy of 100% juice as a dietary equivalent to whole fruit in providing beneficial antioxidants. antioxi ...201323810279
using choice experiments to understand household tradeoffs regarding pineapple production and environmental management in costa rica.choices among environmental management alternatives involve tradeoffs where, for example, the benefits of environmental protection may be offset by economic costs or welfare losses to individual agents. understanding individual or household-level preferences regarding these tradeoffs is not always straightforward, and it often requires an analysis of choices under alternative scenarios. a household survey was used to gather data for a choice experiment, where respondents were asked to choose amo ...201323807434
the expression patterns of bromelain and accys1 correlate with blackheart resistance in pineapple fruits submitted to postharvest chilling stress.blackheart is a physiological disorder induced by postharvest chilling storage during pineapple fruit export shipping. the aim of this study was to check the involvement of bromelain, the cysteine protease protein family abundantly present in pineapple fruits, and accys1, an endogenous inhibitor of bromelain, in the development of blackheart. for this we checked the response to postharvest chilling treatment of two pineapple varieties (md2 and smooth cayenne) differing in their resistance to bla ...201323777839
the influence of total solids content and initial ph on batch biohydrogen production by solid substrate fermentation of agroindustrial wastes.hydrogen is a valuable clean energy source, and its production by biological processes is attractive and environmentally sound and friendly. in méxico 5 million tons/yr of agroindustrial wastes are generated; these residues are rich in fermentable organic matter that can be used for hydrogen production. on the other hand, batch, intermittently vented, solid substrate fermentation of organic waste has attracted interest in the last 10 years. thus the objective of our work was to determine the eff ...201323732191
is pomegranate juice a potential perpetrator of clinical drug-drug interactions? review of the in vitro, preclinical and clinical evidence.the area of fruit juice-drug interaction has received wide attention with numerous scientific and clinical investigations performed and reported for scores of drugs metabolized by cyp3a4/cyp2c9. while grapefruit juice has been extensively studied with respect to its drug-drug interaction potential, numerous other fruit juices such as cranberry juice, orange juice, grape juice, pineapple juice and pomegranate juice have also been investigated for its potential to show drug-drug interaction of any ...201323673492
pineapple juice ingestion for gastric discomfort in diabetic gastroparesis. 201323650790
serum melatonin levels and antioxidant capacities after consumption of pineapple, orange, or banana by healthy male volunteers.melatonin is a naturally occurring molecule biosynthesized by the pineal gland of vertebrates; it also has been identified in many plants. it is considered an important antioxidant and may retard the development of some neurodegenerative diseases and cancer. previous studies in humans have measured melatonin metabolites in urine and have indicated that melatonin-containing foods may provide dietary melatonin. this study tested whether the consumption of fruits or fruit juice containing melatonin ...201323137025
replacement levels of elephant grass by moist pineapple by-product silage in diets of santa inês crossbred sheep: performance and digestibility.the present study evaluated the effect of replacing elephant grass (eg) with moist pineapple by-product silage (ps) on the apparent digestibility, consumption of digestible nutrients and performance of 25 castrated male lambs santa ines crossbreds. the lambs had an initial body weight of 20.2 ± 3.5 kg and were housed in individual pens in a completely randomised design with five treatments (replacement of eg by ps at five proportions of 0, 25, 50, 75 and 100 %) and five replicates during 74 days ...201323001582
enzymatic, antimicrobial and toxicity studies of the aqueous extract of ananas comosus (pineapple) crown leaf.various parts of the plant pineapple (ananas comosus) are used in traditional medicine worldwide for treatment of a number of diseases and disorders. in folk medicine, pineapple leaf extract was used as an antimicrobial, vermicide, purgative, emmenagoogue, abortifacient, anti-oedema and anti-inflammatory agent. compared to the fruit and stem extracts of pineapple, information about its leaf extract is limited. the potential of pineapple crown leaf extract as an ethno-medicine has been evaluated ...201324076462
separation of a binary mixture of pesticides in fruits using a flow-through optosensor.a flow-through optosensor is here proposed for the determination of mixtures of two widely used pesticides, carbendazim and o-phenylphenol, in fruits. the pesticides are separated on-line using an additional amount of solid support, c18 silica gel, in the flow-through cell. the resolution is performed due to the different retention/desorption kinetics of the analytes when interacting with the c18 microbeads. therefore, both separation and determination are integrated in the same cell, considerab ...201324054619
bromelain purification through unconventional aqueous two-phase system (peg/ammonium sulphate).this paper focuses on the feasibility of unconventional aqueous two-phase systems for bromelain purification from pineapple processing waste. the main difference in comparison with conventional systems is the integration of the liquid-liquid extraction technique with fractional precipitation, which can decrease the protein content with no loss of biological activity by removing of unwanted molecules. the analysis of the results was based on the response surface methodology and revealed that the ...201322763776
development and optimisation by means of sensory analysis of new beverages based on different fruit juices and sherry wine vinegar.despite the long history of sherry wine vinegar, new alternatives of consumption are being developed, with the aim of diversifying its market. several new acetic-based fruit juices have been developed by optimising the amount of sherry wine vinegar added to different fruit juices: apple, peach, orange and pineapple. once the concentrations of wine vinegar were optimised by an expert panel, the aforementioned new acetic fruit juices were tasted by 86 consumers. three different aspects were taken ...201322806566
gradual surface degradation of restorative materials by acidic agents.the aim of this study was to investigate the effect of acidic agents on surface roughness and characteristics of four restorative materials. fifty-two discs were created from each restorative material: metal-reinforced glass ionomer cement (ketac-s), resin-modified glass ionomer cement (fuji ii lc), resin composite (filtek z250), and amalgam (valiant-phd); each disc was 12 mm in diameter and 2.5 mm thick. the specimens were divided into four subgroups (n=13) and immersed for 168 hours in four st ...201321903509
modification of pineapple peel fibre with succinic anhydride for cu2+, cd2+ and pb2+ removal from aqueous solutions.research on chemical modification of pineapple peel fibre with succinic anhydride was carried out to create a novel adsorbent for cu2+, cd2+ and pb2+ removal from aqueous solution. after pretreatment with iso-propyl alcohol and naoh, pineapple peel fibre was modified via reaction with succinic anhydride for introduction of carboxylic functional groups. the modified pineapple peel fibre was characterized with fourier transform infrared (ftir) spectroscopy and evaluated for its adsorptive ability ...201321879549
determination of total sulfite in shrimp, potatoes, dried pineapple, and white wine by flow injection analysis: collaborative study.a method for the determination of total sulfite in shrimp, potatoes, dried pineapple, and white wine by flow injection analysis (fia) was collaboratively studied by 8 laboratories. in the method, the sample solution is reacted with sodium hydroxide to liberate aldehyde-bound sulfite. the sample stream is acidified to produce so2 gas, which diffuses across a teflon membrane in the gas diffusion cell into a flowing stream of malachite green. the degree of discoloration of the malachite green is pr ...20132312511
coprecipitation of proteins with matrix ligands: scaleable protein isolation.matrix ligands are agents for isolating proteins out of dilute crudes by coprecipitating proteins. the ligands have a strong anion sulfonate head which initiates binding to proteins having a positive net charge, zh+ approximately 5-20. initial binding tightens protein conformation and starts to squeeze water from conformationally motile proteins. the tails are stackable hydrophobic organic groups, azoaromatic dyes which draw protein-ligand complexes together. proteins coprecipitate as guests, in ...20139174921
determination of selenium levels in dairy products and drinks by hydride generation atomic absorption spectrometry: correlation with daily dietary intake.the selenium concentrations in different dairy products and drinks in the mediterranean coastal area of southeastern spain have been determined by hydride generation atomic absorption spectrometry. mineralization was carried out with an hno3-hclo4 mixture (4:1) in a thermostatted sand bath. selenium determination was carried out by the standard addition method. mean concentrations obtained in diary products varied from 6.87 ng/g (rice pudding) to 178.90 ng/g (caramel custard), and those in drink ...20139102343
quantification of bioactive compounds in pulps and by-products of tropical fruits from brazil.this study aimed to quantify the levels of resveratrol, coumarin, and other bioactives in pulps and by-products of twelve tropical fruits from brazil obtained during pulp production process. pineapple, acerola, monbin, cashew apple, guava, soursop, papaya, mango, passion fruit, surinam cherry, sapodilla, and tamarind pulps were evaluated as well as their by-products (peel, pulp's leftovers, and seed). total phenolic, anthocyanins, yellow flavonoids, β-carotene and lycopene levels were also deter ...201424054258
fruit juice drinks prevent endogenous antioxidant response to high-fat meal ingestion.high-fat meals (hfm) induce metabolic stress, leading to the activation of protective mechanisms, including inflammation and endogenous antioxidant defences. in the present study, we investigated the effects of antioxidant-rich fruit juice drinks on the endogenous antioxidant response induced by hfm. in a double-blind, cross-over design (10 d washout), fourteen overweight volunteers were randomly assigned to one of the following interventions: hfm+500 ml placebo beverage (hfm-pb, free from fruit ...201423930843
an in vitro study reveals nutraceutical properties of ananas comosus (l.) merr. var. mauritius fruit residue beneficial to diabetes.rapid urbanisation and nutritional transition is fuelling the increased global incidence of type 2 diabetes. pineapple fruit residue was explored for its nutraceutical properties as an alternative or adjunct to currently available treatment regime. ethyl acetate and methanolic extracts of pineapple fruit residue were evaluated for anti-diabetic activity in cell free and cell based systems. specifically, we assessed: (1) antioxidant potential, (2) anti-glycation potential, (3) carbohydrate digest ...201423929507
zinc absorption in brazilian subjects fed a healthy meal.the high amounts of phytic acid present in diets from developing countries are considered as important inhibitors of zinc (zn) absorption. the present study aimed to assess the fractional absorption of zn from a meal containing common brazilian foods using the stable isotope technique.201423634993
food allergy in africa: myth or reality?food allergy has been traditionally perceived as being rare in africa. however, the prevalence of other allergic manifestations such as asthma and atopic dermatitis continue to rise in the higher-income african countries. since the food allergy epidemic in westernized countries has lagged behind that of allergic respiratory conditions, we hypothesize that food allergy is increasing in africa. this article systematically reviews the evidence for food allergy in africa, obtained through searching ...201423179518
postprandial glucose response to selected tropical fruits in normal glucose-tolerant nigerians.the glycemic response to commonly eaten fruits in nigeria has not been reported. therefore, this study assessed the plasma glucose response to selected fruits in nigeria.201421493998
fresh-cut pineapple as a new carrier of probiotic lactic acid bacteria.due to the increasing interest for healthy foods, the feasibility of using fresh-cut fruits to vehicle probiotic microorganisms is arising scientific interest. with this aim, the survival of probiotic lactic acid bacteria, belonging to lactobacillus plantarum and lactobacillus fermentum species, was monitored on artificially inoculated pineapple pieces throughout storage. the main nutritional, physicochemical, and sensorial parameters of minimally processed pineapples were monitored. finally, pr ...201425093163
functional properties of a cysteine proteinase from pineapple fruit with improved resistance to fungal pathogens in arabidopsis thaliana.in plant cells, many cysteine proteinases (cps) are synthesized as precursors in the endoplasmic reticulum, and then are subject to post-translational modifications to form the active mature proteinases. they participate in various cellular and physiological functions. here, accp2, a cp from pineapple fruit (ananas comosus l.) belonging to the c1a subfamily is analyzed based on the molecular modeling and homology alignment. transcripts of accp2 can be detected in the different parts of fruits (p ...201424566309
functional characterization of recombinant bromelain of ananas comosus expressed in a prokaryotic system.bromelain (brm) is a defense protein present in the fruit and stem of pineapple (ananas comosus) and it is grouped as a cysteine protease enzyme with diversified medicinal uses. based on its therapeutic applications, bromelain has got sufficient attention in pharmaceutical industries. in the present study, the full coding gene of bromelain in pineapple stem (1,093 bp) was amplified by rt-pcr. the pcr product was cloned, sequenced, and characterized. the sequence analysis of the gene revealed the ...201423921698
highly thermostable and ph-stable cellulases from aspergillus niger ns-2: properties and application for cellulose hydrolysis.optimization of cultural conditions for enhanced cellulase production by aspergillus niger ns-2 were studied under solid-state fermentation. significant increase in yields (cmcase 463.9 ± 20.1 u/g, fpase 101.1 ± 3.5 u/g and β-glucosidase 99 ± 4.0 u/g) were obtained under optimized conditions. effect of different nutritional parameters was studied to induce the maximum production of cellulase complex. scale-up studies for enzyme production process were carried out. characterization studies showed ...201424052336
in vitro evaluation of antibacterial efficacy of pineapple extract (bromelain) on periodontal pathogens.periodontitis is an inflammatory disease resulting in the destruction of periodontal tissues. various treatment modalities have been tried in the form of mechanical therapy and surgical therapy. antimicrobial agents have been used as a monotherapy and as an adjunct with mechanical debridement. various plant extracts have been used as antibacterial agents. pineapple extract (bromelian) is one such agent. hence this study was conducted to assess the antibacterial efficacy of bromelain on both aero ...201425395802
draft genome sequence of pantoea ananatis strain lmg 2665t, a bacterial pathogen of pineapple fruitlets.we report the draft genome sequence of pantoea ananatis lmg 2665(t), the bacterial causal agent of pineapple fruitlet rot.201424855311
the sugarcane defense protein sugarwin2 causes cell death in colletotrichum falcatum but not in non-pathogenic fungi.plants respond to pathogens and insect attacks by inducing and accumulating a large set of defense-related proteins. two homologues of a barley wound-inducible protein (barwin) have been characterized in sugarcane, sugarwin1 and sugarwin2 (sugarcane wound-inducible proteins). induction of sugarwins occurs in response to diatraea saccharalis damage but not to pathogen infection. in addition, the protein itself does not show any effect on insect development; instead, it has antimicrobial activitie ...201424608349
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