Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| androgenesis in solanaceae. | the solanaceae is one of the most important families for global agriculture. among the different solanaceous species, tobacco (nicotiana tabacum), potato (solanum tuberosum), tomato (solanum lycopersicum), eggplant (solanum melongena), and pepper (capsicum annuum) are five crops of outstanding importance worldwide. in these crops, maximum yields are produced by hybrid plants created by crossing pure (homozygous) lines with the desired traits. pure lines may be produced by conventional breeding m ... | 2016 | 26619864 |
| sequence-dependent but not sequence-specific pirna adhesion traps mrnas to the germ plasm. | the conserved piwi family of proteins and piwi-interacting rnas (pirnas) have a central role in genomic stability, which is inextricably linked to germ-cell formation, by forming piwi ribonucleoproteins (pirnps) that silence transposable elements. in drosophila melanogaster and other animals, primordial germ-cell specification in the developing embryo is driven by maternal messenger rnas and proteins that assemble into specialized messenger ribonucleoproteins (mrnps) localized in the germ (pole) ... | 2016 | 26950602 |
| entomopathogenic potential of trichoderma longibrachiatum and its comparative evaluation with malathion against the insect pest leucinodes orbonalis. | the widespread use of chemical pesticides in agriculture has led to the pollution of environmental systems and has caused various health disorders in animals and humans. biological pest control is one of the most environmentally friendly methods in modern agriscience. such methods protect crops from pests and do not pollute the environment. a strain of trichoderma longibrachiatum was isolated and identified from the soil environment of the north 24 parganas district, eastern india. a spore suspe ... | 2016 | 26676413 |
| molecular diversity of turncurtoviruses in iran. | turnip curly top virus (tctv) is the only member of the newly established genus turncurtovirus (family geminiviridae). as part of an ongoing study to identify additional plant hosts and the diversity of turncurtoviruses, between 2012 and 2014, we sampled symptomatic turnip plants and other crops in the provinces fars and khorasan razavi (southern and northeastern iran, respectively). infection by turncurtoviruses was tested by pcr and/or rolling-circle amplification (rca) coupled with restrictio ... | 2016 | 26611911 |
| traditional chinese medicine, food therapy, and hypertension control: a narrative review of chinese literature. | despite the lack of english literature about traditional chinese medicine (tcm) food therapy, there is abundant chinese literature about the application of food therapy for hypertension control. this paper summarizes basic concepts of tcm, the principles of food therapy and its application for hypertension control according to chinese literature. in tcm, food is conceptualized according to both nutritional and functional aspects, and can be used to treat illnesses. four principles of tcm food th ... | 2016 | 27852126 |
| leaf morphological characters can be a factor for intra-varietal preference of whitefly bemisia tabaci (hemiptera: aleyrodidae) among eggplant varieties. | the sweetpotato whitefly, bemisia tabaci (hemiptera: aleyrodidae) meam1, is considered a serious pest of horticultural and many other crops. while eggplant (solanum melongena) is one of the most favored host plants, the whiteflies exhibit preferences among different varieties. we hypothesized that certain morphological leaf characteristics of different varieties, like leaf trichome density, trichome length, leaf lamina thickness and leaf color, may affect whitefly landing, feeding and ovipositio ... | 2016 | 27081849 |
| a primary sequence analysis of the argonaute protein family in plants. | small rna (srna)-mediated gene silencing represents a conserved regulatory mechanism controlling a wide diversity of developmental processes through interactions of srnas with proteins of the argonaute (ago) family. on the basis of a large phylogenetic analysis that includes 206 ago genes belonging to 23 plant species, ago genes group into four clades corresponding to the phylogenetic distribution proposed for the ten family members of arabidopsis thaliana. a primary analysis of the correspondin ... | 2016 | 27635128 |
| novel insight into the mechanism underlying light-controlled anthocyanin accumulation in eggplant (solanum melongena l.). | eggplant is rich in anthocyanins, which are the major secondary metabolites and beneficial to human health. we discovered that the anthocyanin biosynthesis of eggplant cultivar 'lanshan hexian' was regulated by light. in this study, we isolated two blue light receptor genes, smcry1 and smcry2, and negative/positive anthocyanin regulatory factors smcop1 and smhy5 from eggplant. in terms of transcript levels, smcry1, smcry2 and smhy5 were up-regulated by light, while smcop1 was down-regulated. sub ... | 2016 | 27297989 |
| identification and characterization of cbl and cipk gene families in eggplant (solanum melongena l.). | eggplant (solanum melongena l.) is an edible vegetable cultivated and consumed worldwide. but the production of eggplant is significantly limited by the soil salinization in greenhouse cultivation system. the main ions are na(+), ca(2+), mg(2+), k(+), cl(-), and so4 (2-) in the salty soils. calcineurin b-like proteins (cbls) are calcium sensors and control the affinities and activities of numerous ion transporters with cbl-interacting protein kinases (cipks). in this study, a total of 5 cbl and ... | 2016 | 27287616 |
| expression of a cystatin transgene in eggplant provides resistance to root-knot nematode, meloidogyne incognita. | root-knot nematodes (rkn) cause substantial yield decline in eggplant and sustainable management options to minimize crop damage due to nematodes are still limited. a number of genetic engineering strategies have been developed to disrupt the successful plant-nematode interactions. among them, delivery of proteinase inhibitors from the plant to perturb nematode development and reproduction is arguably the most effective strategy. in the present study, transgenic eggplant expressing a modified ri ... | 2016 | 27516765 |
| sequence variation of block iii segment identifies three distinct lineages within eggplant mottled dwarf virus isolates from italy, spain and greece. | partial polymerase (l) gene sequences of 919 nts, including the conserved segments pre-motif a and motif a of block iii, of 20 eggplant mottled dwarf virus (emdv) isolates were generated, and trimmed sequences of 889 nts, based on the length of available sequences of other isolates, were used to determine phylogenetic relationships. phylogenetic reconstructions revealed two divergent lineages, designated as genetic group a (italian isolates) and group b, with the latter further divided into subg ... | 2016 | 26982474 |
| pittosporum cryptic virus 1: genome sequence completion using next-generation sequencing. | next-generation sequencing (ngs) was applied to dsrnas extracted from an italian pittosporum plant infected with pittosporum cryptic virus 1 (picv1). ngs allowed assembly of the full genome sequence of picv1, comprising dsrna1 (1.9 kbp) and dsrna2 (1.5 kbp), which encode the rna-dependent rna polymerase and capsid protein genes, respectively. phylogenetic and sequence analyses confirmed that picv1 is a new member of the genus deltapartitivirus, family partiviridae. from the same plant, nsg also ... | 2016 | 27087112 |
| efficacy of various application methods of fluensulfone for managing root-knot nematodes in vegetables. | fluensulfone is a new nematicide in the flouroalkenyl chemical group. a field experiment was conducted in 2012 and 2013 to evaluate the efficacy of various application methods of fluensulfone for control of meloidogyne spp. in cucumber (cucumis sativus). treatments of fluensulfone (3.0 kg a.i./ha) were applied either as preplant incorporation (ppi) or via different drip irrigation methods: drip without pulse irrigation (drip np), pulse irrigation 1 hr after treatment (drip +1p), and treatment at ... | 2016 | 27418698 |
| systemic acquired resistance activation in solanaceous crops as a management strategy against root-knot nematodes. | activators of systemic acquired resistance (sar), such as salicylic acid (sa) and its synthetic functional analogues benzo(1,2,3)thiadiazole-7-carbothionic acid-s-methyl ester (bth) and 2,6-dichloroisonicotinic acid (ina), were tested on tomato, eggplant and pepper for the control of the root-knot nematode meloidogyne incognita. effects on plant fitness, nematode reproduction and root galling were screened in relation to different methods of application, to different applied dosages of chemicals ... | 2016 | 26085141 |
| multifaceted effects of host plants on entomopathogenic nematodes. | the success of parasites can be impacted by multi-trophic interactions. tritrophic interactions have been observed in parasite-herbivore-host plant systems. here we investigate aspects of multi-trophic interactions in a system involving an entomopathogenic nematode (epn), its insect host, and host plant. novel issues investigated include the impact of tritrophic interactions on nematode foraging behavior, the ability of epns to overcome negative tritrophic effects through genetic selection, and ... | 2016 | 26896698 |
| genetic and mechanistic diversity of pirna 3'-end formation. | small regulatory rnas guide argonaute (ago) proteins in a sequence-specific manner to their targets and therefore have important roles in eukaryotic gene silencing. of the three small rna classes, micrornas and short interfering rnas are processed from double-stranded precursors into defined 21- to 23-mers by dicer, an endoribonuclease with intrinsic ruler function. piwi-interacting rnas (pirnas)-the 22-30-nt-long guides for piwi-clade ago proteins that silence transposons in animal gonads-are g ... | 2016 | 27851737 |
| determination of diphenylamine in agricultural products by hplc-fl. | a method for the determination of diphenylamine in agricultural products was developed. diphenylamine was extracted with acetonitrile from a sample under an acidic condition, passed through a c18 cartridge column, re-extracted with n-hexane, cleaned up on a psa cartridge column, determined by hplc with fluorescence detector and confirmed by liquid chromatography with tandem mass spectrometry. average recoveries (n=5) from brown rice, corn, soybeans, potato, cabbage, eggplant, spinach, orange, ap ... | 2016 | 28025454 |
| in vivo mapping of a dynamic ribonucleoprotein granule interactome in early drosophila embryos. | macromolecular complexes and organelles play crucial roles within cells, but their native architectures are often unknown. here, we use an evolutionarily conserved germline organelle, the germ granule, as a paradigm. in drosophila embryos, we map one of its interactomes using a novel in vivo crosslinking approach that employs two interacting granule proteins and determines their common neighbor molecules. we identified an in vivo granule assembly of tudor, aubergine, motor and metabolic proteins ... | 2016 | 28203524 |
| evaluation of suitable reference genes for normalization of qpcr gene expression studies in brinjal (solanum melongena l.) during fruit developmental stages. | brinjal/eggplant/aubergine is one of the major solanaceous vegetable crops. recent availability of genome information greatly facilitates the fundamental research on brinjal. gene expression patterns during different stages of fruit development can provide clues towards the understanding of its biological functions. quantitative real-time pcr (qpcr) has become one of the most widely used methods for rapid and accurate quantification of gene expression. however, its success depends on the use of ... | 2016 | 26472671 |
| cooking influence on physico-chemical fruit characteristics of eggplant (solanum melongena l.). | physico-chemical traits of three eggplant genotypes ("tunisina", "buia" and "l 305") were evaluated before and after two cooking treatments (grilling and boiling). different genotypes revealed different changes after cooking, with "tunisina" showing a better retention of phytochemicals with respect to other two genotypes. the main physical phenomena were water loss during grilling, and dry matter loss after boiling. chlorogenic acid, the main phenolic in eggplant, resulted higher in grilled samp ... | 2016 | 26471625 |
| phylogenetic origin and diversification of rnai pathway genes in insects. | rna interference (rnai) refers to the set of molecular processes found in eukaryotic organisms in which small rna molecules mediate the silencing or down-regulation of target genes. in insects, rnai serves a number of functions, including regulation of endogenous genes, anti-viral defense, and defense against transposable elements. despite being well studied in model organisms, such as drosophila, the distribution of core rnai pathway genes and their evolution in insects is not well understood. ... | 2016 | 28062756 |
| rna interference in the colorado potato beetle, leptinotarsa decemlineata: identification of key contributors. | rna interference (rnai) is a useful reverse genetics tool for investigation of gene function as well as for practical applications in many fields including medicine and agriculture. rnai works very well in coleopteran insects including the colorado potato beetle (cpb), leptinotarsa decemlineata. we used a cell line (lepd-sl1) developed from cpb to identify genes that play key roles in rnai. we screened 50 genes with potential functions in rnai by exposing lepd-sl1 cells to dsrna targeting one of ... | 2016 | 27687845 |
| violet led light enhances the recruitment of a thrip predator in open fields. | the predatory bug orius sauteri is an indigenous natural enemy of thrips and whiteflies in asian countries. to put these bugs to practical use in pest management, methods to attract and retain the bugs in agricultural fields are needed. we previously showed that violet light (405 nm) attracts o. sauteri selectively. many thrips and whiteflies are attracted to uv or green light. in this study, we examined the effect of violet-led illumination on o. sauteri in pesticide-free eggplant (solanum melo ... | 2016 | 27604315 |
| rna helicase spn-e is required to maintain aub and ago3 protein levels for pirna silencing in the germline of drosophila. | germline-specific rna helicase spindle-e (spn-e) is known to be essential for pirna silencing in drosophila that takes place mainly in the perinuclear nuage granules. loss-of-function spn-e mutations lead to tandem stellate genes derepression in the testes and retrotransposon mobilization in the ovaries. however, spn-e functions in the pirna pathway are still obscure. analysis of total library of short rnas from the testes of spn-e heterozygous flies revealed the presence of abundant pirna ping- ... | 2016 | 27320195 |
| phenotyping of eggplant wild relatives and interspecific hybrids with conventional and phenomics descriptors provides insight for their potential utilization in breeding. | eggplant (solanum melongena) is related to a large number of wild species that are a source of variation for breeding programmes, in particular for traits related to adaptation to climate change. however, wild species remain largely unexploited for eggplant breeding. detailed phenotypic characterization of wild species and their hybrids with eggplant may allow identifying promising wild species and information on the genetic control and heterosis of relevant traits. we characterizated six eggpla ... | 2016 | 27242876 |
| short-term water deficit changes cuticular sterol profile in the eggplant (solanum melongena). | crop irrigation uses a majority of a total world water supply, at the same time displaying low efficiency. as the expected, future water requirements are higher than the current ones; there is a risk of a growing deficit of water for the agricultural use. hence, there is an arising need for better understanding the effects of water deprivation on the crop plants. eggplant (solanum melongena l.) is a vegetable crop cultivated in arid and semi-arid parts of the world. because of its high water dem ... | 2016 | 27127890 |
| spindle-e cycling between nuage and cytoplasm is controlled by qin and piwi proteins. | transposable elements (tes) are silenced in germ cells by a mechanism in which piwi proteins generate and use piwi-interacting ribonucleic acid (pirna) to repress expression of te genes. pirna biogenesis occurs by an amplification cycle in microscopic organelles called nuage granules, which are localized to the outer face of the nuclear envelope. one cofactor required for amplification is the helicase spindle-e (spn-e). we found that the spn-e protein physically associates with the tudor domain ... | 2016 | 27091448 |
| novel mutants of the aubergine gene. | aubergine is an rna-binding protein of the piwi clade, functioning in germline in the pirna pathway that silences transposons and repetitive sequences. several mutations of this gene exist, but they mostly result in truncated proteins or correspond to mutations that also affect neighboring genes. we have generated complete aubergine knock-out mutants that do not disrupt the neighboring genes. these novel mutants are characterized by pcr and sequencing. their nature is confirmed by female sterili ... | 2016 | 27064345 |
| mrnas hit a sticky wicket. | drosophila germ cell specification depends on localization of mrnas required for patterning to the posterior of the oocyte during oogenesis. in a recent issue of nature, vourekas et al. (2016) suggest that aubergine in complex with pirnas may provide a low-specificity anchoring mechanism for posterior mrnas. | 2016 | 27046827 |
| crop wild relatives of the brinjal eggplant (solanum melongena): poorly represented in genebanks and many species at risk of extinction. | crop wild relatives (cwr) provide important traits for plant breeding, including pest, pathogen, and abiotic stress resistance. therefore, their conservation and future availability are essential for food security. despite this need, the world's genebanks are currently thought to conserve only a small fraction of the total diversity of cwr. | 2016 | 27026215 |
| cobalt oxide nanoparticles aggravate dna damage and cell death in eggplant via mitochondrial swelling and no signaling pathway. | despite manifold benefits of nanoparticles (nps), less information on the risks of nps to human health and environment has been studied. cobalt oxide nanoparticles (co3o4-nps) have been reported to cause toxicity in several organisms. in this study, we have investigated the role of co3o4-nps in inducing phytotoxicity, cellular dna damage and apoptosis in eggplant (solanum melongena l. cv. violetta lunga 2). to the best of our knowledge, this is the first report on co3o4-nps showing phytotoxicity ... | 2016 | 26988690 |
| mapping quantitative trait loci affecting biochemical and morphological fruit properties in eggplant (solanum melongena l.). | eggplant berries are a source of health-promoting metabolites including antioxidant and nutraceutical compounds, mainly anthocyanins and chlorogenic acid; however, they also contain some anti-nutritional compounds such as steroidal glycoalkaloids (sga) and saponins, which are responsible for the bitter taste of the flesh and with potential toxic effects on humans. up to now, quantitative trait loci (qtl) for the metabolic content are far from being characterized in eggplant, thus hampering the a ... | 2016 | 26973692 |
| solanum melongena polyphenol oxidase biosensor for the electrochemical analysis of paracetamol. | a new strategy for the construction of a polyphenol oxidase carbon paste biosensor for paracetamol detection is reported. the eggplant (solanum melongena) was processed to collect the polyphenol oxidase as an enzyme that was incorporated in the carbon paste sensor construction. the constructed sensor displayed high sensitivity and good selection for paracetamol detection and recognition. optimized conditions included ph 6.0 (highest activity), ph 7.0 (highest stability), pulse amplitude of 50 mv ... | 2016 | 26930128 |
| solanidine and tomatidine trigger scar pruritus. | scar pruritus is frequently encountered in clinical practice (particularly in burn patients) owing to its poorly known pathogenesis and difficult treatment. in previous work, we demonstrated the usefulness of a diet excluding edible solanaceae (viz., potatoes, tomatoes, peppers and aubergines) in patients with antihistamine-resistant scar pruritus. we hypothesized that alkaloids in solanaceae (particularly their secondary metabolites or aglycones) might be the actual pruritogens. in order to tes ... | 2016 | 26777454 |
| optimization of ultrasound-assisted extraction of total monomeric anthocyanin (tma) and total phenolic content (tpc) from eggplant (solanum melongena l.) peel. | the present study describes the extraction of total monomeric anthocyanin (tma) and total phenolic content (tpc) from eggplant peel using ultrasonic treatments and methanol and 2-propanol as extraction solvents. the extraction yields were optimized by varying the solvent concentration, ultrasonic frequency, temperature and time of ultrasonic treatment. box-behnken design was used to investigate the effect of process variables on the ultrasound-assisted extraction. the results showed that for tpc ... | 2016 | 26701808 |
| the effect of organic fertilizers and flowering plants on sheet-web and wolf spider populations (araneae: lycosidae and linyphiidae) and its importance for pest control. | the main goal of this study was to identify the treatment that increases the populations of spiders, which are effective predators in agroecosystems. in 2013 and 2014 the experimental eggplant (solanum melongena l.) field was two different treatments, organic fertilizers and chemical fertilizer treatment, and in 2014 we surrounded organic fertilizer plots with the flowering plants mealy cup sage (salvia farinacea benth.), spearmint (mentha spicata l.), and basil (ocimum basilicum l.). analysis u ... | 2016 | 28076280 |
| anther culture in eggplant (solanum melongena l.). | the technique of in vitro anther culture is the most favorite to incite the production of plants from microspore through direct embryogenesis or regeneration from callus. anther culture has been employed since 1980s in eggplant to obtain double-haploid plants from microspore derived embryos. from that time it has been refined and widely applied both at commercial level for a fast generation double-haploid parental lines of f1 hybrids, as well as for experimental studies as the complete homozygos ... | 2016 | 26619880 |
| transcriptome analysis of solanum melongena l. (eggplant) fruit to identify putative allergens and their epitopes. | eggplant is the third most important solanaceae crop after tomato and potato, particularly in india and china. a transcriptome analysis of eggplant's fruit was performed to study genes involved in medicinal importance and allergies. illumina hiseq 2000 system generated 89,763,638 raw reads (~18 gb) from eggplant. high quality reads (59,039,694) obtained after trimming process, were assembled into a total of 149,224 non redundant set of transcripts. out of 80,482 annotated sequences of eggplant f ... | 2016 | 26424595 |
| green nanoparticles engineering on root-knot nematode infecting eggplants and their effect on plant dna modification. | root-knot nematodes are known to cause significant damage to eggplants. new approaches by green silver nanoparticles (gsn) are used to control plant-parasitic nematode to avoid chemical nematicide hazards. | 2016 | 28959343 |
| an in vivo proteomic analysis of the me31b interactome in drosophila germ granules. | drosophila me31b is a conserved protein of germ granules, ribonucleoprotein complexes essential for germ cell development. me31b post-transcriptionally regulates mrnas by interacting with other germ granule proteins. however, a me31b interactome is lacking. here, we use an in vivo proteomics approach to show that the me31b interactome contains polypeptides from four functional groups: rna regulatory proteins, glycolytic enzymes, cytoskeleton/motor proteins, and germ plasm components. we further ... | 2017 | 28945271 |
| in vitro culture responses, callus growth and organogenetic potential of brinjal (solanum melongena l.) to he-ne laser irradiation. | the present investigation was designed to analyze the influence of helium-neon (he-ne 632.8nm) laser irradiation on defense enzymes, proline content and in vitro responses of callus induction, shoot initiation and on plantlet regeneration potential of brinjal. the seeds of mattu gulla (solanum melongena l.) were irradiated with 20, 25 and 30j/cm2 of he-ne laser followed by surface sterilization and sprouted on murashige and skoog medium without plant growth regulators. the activity of defense en ... | 2017 | 28818779 |
| profiling and quantification of regioisomeric caffeoyl glucoses in solanaceae vegetables. | based on the recently developed tandem ms based hierarchical scheme for the identification of regioisomeric caffeoyl glucoses, selected vegetables were profiled with respect to their caffeoyl glucose content. the dietary plants profiled were tomato, pepper, chilli and aubergine, all members of the solanaceae family. 6-o-caffeoyl glucose was found to be the predominant isomer. in processed food such as tomato puree and ketchup a larger number of caffeoyl-glucose isomers formed through acyl migrat ... | 2017 | 28764050 |
| aubergine and pirnas promote germline stem cell self-renewal by repressing the proto-oncogene cbl. | piwi proteins play essential roles in germ cells and stem cell lineages. in drosophila, piwi is required in somatic niche cells and germline stem cells (gscs) to support gsc self-renewal and differentiation. whether and how other piwi proteins are involved in gsc biology remains unknown. here, we show that aubergine (aub), another piwi protein, is intrinsically required in gscs for their self-renewal and differentiation. aub needs to be loaded with pirnas to control gsc self-renewal and acts thr ... | 2017 | 29030484 |
| demographic comparison of henosepilachna vigintioctopunctata (f.) (coleoptera: coccinellidae) reared on three cultivars of solanum melongena l. and a wild hostplant solanum nigrum l. | henosepilachna vigintioctopunctata (f.) is a widespread pest found on many solanaceous vegetables. the development and fecundity of h. vigintioctopunctata were studied on three cultivars ('zihong', 'eyou', and 'baijiao') of eggplant, solanum melongena l., using the two-sex life table theory. these results were then compared with similar parameters from a wild alternate host, solanum nigrum. our results showed that there were no significant differences between the developmental times of the larva ... | 2017 | 28961786 |
| pirnas and aubergine cooperate with wispy poly(a) polymerase to stabilize mrnas in the germ plasm. | piwi-interacting rnas (pirnas) and piwi proteins play a crucial role in germ cells by repressing transposable elements and regulating gene expression. in drosophila, maternal pirnas are loaded into the embryo mostly bound to the piwi protein aubergine (aub). aub targets maternal mrnas through incomplete base-pairing with pirnas and can induce their destabilization in the somatic part of the embryo. paradoxically, these aub-dependent unstable mrnas encode germ cell determinants that are selective ... | 2017 | 29101389 |
| world vegetable center eggplant collection: origin, composition, seed dissemination and utilization in breeding. | eggplant is the fifth most economically important solanaceous crop after potato, tomato, pepper, and tobacco. apart from the well-known brinjal eggplant (solanum melongena l.), two other under-utilized eggplant species, the scarlet eggplant (s. aethiopicum l.) and the gboma eggplant (s. macrocarpon l.) are also cultivated. the taxonomy and identification of eggplant wild relatives is challenging for breeders due to the large number of related species, but recent phenotypic and genetic data and c ... | 2017 | 28970840 |
| influence of root exudates and soil on attachment of pasteuria penetrans to meloidogyne arenaria. | the bacterium pasteuria penetrans is a parasite of root-knot nematodes (meloidogyne spp.). endospores of p. penetrans attach to the cuticle of second-stage juveniles (j2) and subsequently sterilize infected females. when encumbered by large numbers of spores, juveniles are less mobile and their ability to infect roots is reduced. this study looked at different factors that influence spore attachment of p. penetrans to the root-knot nematode meloidogyne arenaria. pretreatment of j2 with root exud ... | 2017 | 29062153 |
| distinct fitness costs associated with the knockdown of rnai pathway genes in western corn rootworm adults. | rna interference (rnai) based approaches can potentially be used to control insect pests. these approaches may depend on the usage of microrna (mirna) or double stranded rna (dsrna) mediated gene knockdown, which likely involves proteins that regulate these pathways, such as argonaute 1 (ago1), argonaute 2 (ago2), dicer 1 (dcr1), dicer 2 (dcr2), and drosha in insects. we previously performed functional characterization of ago2 and dcr2 of western corn rootworm (wcr), diabrotica virgifera virgife ... | 2017 | 29267401 |
| comparative proteomic analysis of eggplant (solanum melongena l.) heterostylous pistil development. | heterostyly is a common floral polymorphism, but the proteomic basis of this trait is still largely unexplored. in this study, self- and cross-pollination of l-morph and s-morph flowers and comparison of embryo sac development in eggplant (solanum melongena l.) suggested that lower fruit set from s-morph flowers results from stigma-pollen incompatibility. to explore the molecular mechanism underlying heterostyly development, we conducted isobaric tags for relative and absolute quantification (it ... | 2017 | 28586360 |
| oxylipin mediated stress response of a miraculin-like protease inhibitor in hexanoic acid primed eggplant plants infested by colorado potato beetle. | insect-plant interactions are governed by a complex equilibrium between the mechanisms through which plant recognize insect attack and orchestrate downstream signaling events that trigger plant defense responses, and the mechanisms by which insects overcome plant defenses. due to this tight and dynamic interplay, insight into the nature of the plant defense response can be gained by analyzing changes in the insect herbivores digestive system upon plant feeding. in this work we have identified a ... | 2017 | 28578135 |
| emerging food allergens: identification of polyphenol oxidase as an important allergen in eggplant (solanum melongena l.). | although many allergens have been detected in eggplant (solanum melongena l.), their identity have not been elucidated. the aim of this study was to investigate whether polyphenol oxidase (ppo), an important eggplant enzyme, acts as an allergen. the proteins of eggplant peel extract were separated on phenyl-sepharose (ps), and analyzed by skin prick test (spt), elisa and ige-immunoblotting; the components were analyzed for ppo activity, presence of protein-bound copper, and recognition by rabbit ... | 2017 | 28164808 |
| phenolics content, fruit flesh colour and browning in cultivated eggplant, wild relatives and interspecific hybrids and implications for fruit quality breeding. | increasing the content in bioactive phenolics in the eggplant (solanum melongena) fruit is of interest, but may result in enhanced browning. we evaluated six varieties of s. melongena, 22 accessions of wild related species and 42 interspecific hybrids between cultivated eggplant and wild relatives for phenolics content, fruit flesh colour, polyphenol oxidase (ppo) activity, and fruit flesh browning. wild relatives generally had a higher content in phenolics and a broader range of variation than ... | 2017 | 29195964 |
| leaf chemical compositions of different eggplant varieties affect performance of bemisia tabaci (hemiptera: aleyrodidae) nymphs and adults. | the sweetpotato whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae) meam1 is a serious pest of many crops worldwide, and its control mostly depends on insecticides. one of the most preferred host plants of b. tabaci is eggplant, solanum melongena, although preferences among different cultivars of the whiteflies vary. we hypothesized that certain nutritional and defensive chemicals of plant leaves, such as nitrogen, glucose, fructose, sucrose, amino acids, total phenolic components, and ... | 2017 | 29272524 |
| comparative transcriptome analysis provides insights into molecular mechanisms for parthenocarpic fruit development in eggplant (solanum melongena l.). | genetic control of parthenocarpy, a desirable trait in edible fruit with hard seeds, has been extensively studied. however, the molecular mechanism of parthenocarpic fruit development in eggplant (solanum melongena l.) is still unclear. to provide insights into eggplant parthenocarpy, the transcriptomic profiles of a natural parthenocarpic (pp05) and two non-parthenocarpic (pnp05 and gnp05) eggplant lines were analyzed using rna-sequencing (rna-seq) technology. these sequences were assembled int ... | 2017 | 28604820 |
| the pax protein eyegone (eyg) interacts with the pi-rna component aubergine (aub) and controls egg chamber development in drosophila. | the eyegone (eyg) gene encodes eyg, a transcription factor of the pax family with multiple roles during drosophila development. eyg has been shown to be nuclear in the cells where it functions. in this report we describe a new functional cytoplasmic distribution of eyg during egg chamber development in the female ovarioles. the protein is present from the germarium until stage 10 of cyst development. the majority of egg chambers that develop in the absence of eyg arrest their development before ... | 2017 | 29278721 |
| coding snps analysis highlights genetic relationships and evolution pattern in eggplant complexes. | brinjal (solanum melongena), scarlet (s. aethiopicum) and gboma (s. macrocarpon) eggplants are three old world domesticates. the genomic dna of a collection of accessions belonging to the three cultivated species, along with a representation of various wild relatives, was characterized for the presence of single nucleotide polymorphisms (snps) using a genotype-by-sequencing approach. a total of 210 million useful reads were produced and were successfully aligned to the reference eggplant genome ... | 2017 | 28686642 |
| combined transcriptomic and proteomic analysis constructs a new model for light-induced anthocyanin biosynthesis in eggplant (solanum melongena l.). | light is a key environmental factor affecting anthocyanin biosynthesis. our previous study demonstrated that "lanshan hexian" is a light-sensitive eggplant cultivar, but its regulatory mechanism is unknown. here, delphinidin-3-[4-(cis-p-coumaroyl)-rhamnosyl-glucopyranoside]-5-glucopyranoside and delphinidin-3-[4-(trans-p-coumaroyl)-rhamnosyl-glucopyranoside]-5-glucopyranoside were identified as the main anthocyanin components in lanshan hexian by ultra-performance liquid chromatography-tandem ma ... | 2017 | 28940206 |
| development and genetic characterization of advanced backcross materials and an introgression line population of solanum incanum in a s. melongena background. | advanced backcrosses (abs) and introgression lines (ils) of eggplant (solanum melongena) can speed up genetics and genomics studies and breeding in this crop. we have developed the first full set of abs and ils in eggplant using solanum incanum, a wild eggplant that has a relatively high tolerance to drought, as a donor parent. the development of these abs and il eggplant populations had a low efficiency in the early stages, because of the lack of molecular markers and genomic tools. however, th ... | 2017 | 28912788 |
| characterization and synergistic effect of antifungal volatile organic compounds emitted by the geotrichum candidum pf005, an endophytic fungus from the eggplant. | plant-associated endophytes are recognized as sources of novel bioactive molecules having diverse applications. in this study, an endophytic yeast-like fungal strain was isolated from the fruit of eggplant (solanum melongena) and identified as geotrichum candidum through phenotypic and genotypic characterizations. this endophytic g. candidum isolate pf005 was found to emit fruity scented volatiles. the compositional profiling of volatile organic compounds (vocs) revealed the presence of 3-methyl ... | 2017 | 28894891 |
| crystal structure of nonspecific lipid transfer protein from solanum melongena. | lipids are considered to protect protein allergens from proteolysis and are generally seen to exist in a bound form. one of the well-known plant protein families with bound lipids is non-specific lipid transfer proteins (nsltps). structure-function relationships in the case of the members of non-specific lipid transfer protein family are not clearly understood. as part of exploring the seed proteome, we have analyzed the proteome of a member of solanaceae family, solanum melongena (eggplant) and ... | 2017 | 28612368 |
| evaluation of solasonine content and expression patterns of sgt1 gene in different tissues of two iranian eggplant (solanum melongena l.) genotypes. | eggplant (solanum melongena l.) is one of the most consumed vegetables in the world. the eggplant glycoalkaloids (gas) are toxic secondary metabolites that may have detrimental effects on human health, particularly if the magnitudes of gas are higher than the recommended food safety level (200 mg per kg of fresh mass). in this study, the content of solasonine compound and the expression patterns of solasodine galactosyltransferase (sgt1) gene were assessed in different tissues (mature leaves, fl ... | 2017 | 28867954 |
| zucchini-dependent pirna processing is triggered by recruitment to the cytoplasmic processing machinery. | the pirna pathway represses transposable elements in the gonads and thereby plays a vital role in protecting the integrity of germline genomes of animals. mature pirnas are processed from longer transcripts, pirna precursors (pre-pirnas). in drosophila, processing of pre-pirnas is initiated by pirna-guided slicer cleavage or the endonuclease zucchini (zuc). as zuc does not have any sequence or structure preferences in vitro, it is not known how pirna precursors are selected and channeled into th ... | 2017 | 29021243 |
| effects of grilling on total polyphenol content and antioxidant capacity of eggplant (solanum melongena l.). | cooking can change the polyphenol contents of eggplant. this study elucidated the effects of grilling on total polyphenol content (tpc), antioxidant capacity, and the inner structures of eggplant. after identical hollowing, cylindrical eggplant samples were prepared and were then grilled until their center temperatures (ct) respectively reached 50, 65, 75, 85, and 95 °c. chemical assays and observations of the inner structures clarified that tpc and 1, 1-diphenyl-2-picrylhydrazyl (dpph) radical ... | 2017 | 27973738 |
| acizzia solanicola (hemiptera: psyllidae) probing behaviour on two solanum spp. and implications for possible pathogen spread. | piercing-sucking insects are vectors of plant pathogens, and an understanding of their feeding behaviour is crucial for studies on insect population dynamics and pathogen spread. this study examines probing behaviour of the eggplant psyllid, acizzia solanicola (hemiptera: psyllidae), using the electrical penetration graph (epg) technique, on two widespread and common hosts: eggplant (solanum melongena) and tobacco bush (s. mauritianum). six epg waveforms were observed: waveform np (non-probing p ... | 2017 | 28575085 |
| aubergine controls germline stem cell self-renewal and progeny differentiation via distinct mechanisms. | piwi family protein aubergine (aub) maintains genome integrity in late germ cells of the drosophila ovary through piwi-associated rna-mediated repression of transposon activities. although it is highly expressed in germline stem cells (gscs) and early progeny, it remains unclear whether it plays any roles in early gsc lineage development. here we report that aub promotes gsc self-renewal and gsc progeny differentiation. rna-iclip results show that aub binds the mrnas encoding self-renewal and di ... | 2017 | 28441530 |
| a spiking strategy facilitates housekeeping selection for rt-qpcr analysis under different biotic stresses in eggplant. | endogenous housekeeping genes are traditionally employed to normalize the expression of target genes in rt-qpcr studies. assuming that a perfect housekeeping suitable for every condition does not exist, expression stability of the chosen reference gene should be evaluated at every new experiment. the housekeeping selection process reveals furthermore complicated and time-consuming when different conditions have to be compared in the same experimental dataset. as an alternative strategy, we spike ... | 2017 | 28429149 |
| solasodine inhibits invasion of human lung cancer cell through downregulation of mir-21 and mmps expression. | solasodine, a naturally occurring aglycone of glycoalkaloid in eggplant (solanum melongena), was found to inhibit proliferation in various tumor cells. however, the effect of solasodine on cancer cell metastasis remains unclear. this study investigates the suppression mechanism of solasodine on motility of human lung cancer cell a549 in vitro. results show that solasodine reduces viability of a549 cells. treatment with non-toxic doses of solasodine suppresses markedly cell invasion. solasodine r ... | 2017 | 28283413 |
| investigation of orthologous pathogen recognition gene-rich regions in solanaceous species. | pathogen receptor proteins such as receptor-like protein (rlp), receptor-like kinase (rlk), and nucleotide-binding leucine-rich repeat (nlr) play a leading role in plant immunity activation. the genome architecture of such genes has been extensively investigated in several plant species. however, we still know little about their elaborate reorganization that arose during the plant speciation process. using recently released pepper and eggplant genome sequences, we were able to identify 1097 path ... | 2017 | 28742982 |
| transcriptional polymorphism of pirna regulatory genes underlies the mariner activity in d. simulans testes. | during colonization of new areas, natural populations have to deal with changing environments, and transposable elements (tes) can be useful "tools" in the adaptation process since they are major contributor to the structural and functional evolution of genomes. in this general context, the activity (copy number, transcriptional and excision rate) of the mariner mos1 element was estimated in 19 natural populations of d. simulans. it is shown (1) that mos1 expression is always higher and more var ... | 2017 | 28401606 |
| salicylic acid seed priming instigates defense mechanism by inducing pr-proteins in solanum melongena l. upon infection with verticillium dahliae kleb. | salicylic acid (sa) is a hormone connected with various cellular functions including the fight against invading pathogens. priming of seeds pre-sowing is a very simple method to the farmers' to produce better growth, yield and manage the pathogens. the present study was aimed to determine the growth and disease resistance ability in brinjal seeds primed with different concentrations (0.25, 0.5, 0.75 and 1.0 mm) of sa under greenhouse conditions. priming of seeds with sa significantly increased s ... | 2017 | 28578205 |
| eggplant resistance to the ralstonia solanacearum species complex involves both broad-spectrum and strain-specific quantitative trait loci. | bacterial wilt (bw) is a major disease of solanaceous crops caused by the ralstonia solanacearum species complex (rssc). strains are grouped into five phylotypes (i, iia, iib, iii, and iv). varietal resistance is the most sustainable strategy for managing bw. nevertheless, breeding to improve cultivar resistance has been limited by the pathogen's extensive genetic diversity. identifying the genetic bases of specific and non-specific resistance is a prerequisite to breed improvement. a major gene ... | 2017 | 28580001 |
| a putative r3 myb repressor is the candidate gene underlying atroviolacium, a locus for anthocyanin pigmentation in tomato fruit. | anthocyanins are potential health-promoting compounds in the human diet. the atv (atroviolacium) locus, derived from the wild tomato species solanum cheesmaniae, has been shown to enhance anthocyanin pigmentation in tomato fruit when it co-exists with either the aft (anthocyanin fruit) or the abg (aubergine) locus. in the present study, the atv locus was fine-mapped to an approximately 5.0-kb interval on chromosome 7. a putative r3 myb repressor was identified in this interval and is hereby desi ... | 2017 | 29186488 |
| multivariate analysis as a key tool in chemotaxonomy of brinjal eggplant, african eggplants and wild related species. | the brinjal eggplant (solanum melongena l.) is an important vegetable species worldwide, while african eggplants (s. aethiopicum l., s. macrocarpon l.) are indigenous vegetable species of local significance. taxonomy of eggplants and their wild relatives is complicated and still unclear. hence, the objective of the study was to clarify taxonomic position of cultivars and landraces of brinjal, its wild relatives and african eggplant species and their wild ancestors using chemotaxonomic markers an ... | 2017 | 28910606 |
| performance of pseudapanteles dignus (hymenoptera: braconidae), a natural enemy of tuta absoluta (lepidoptera: gelechiidae) in eggplant. | pseudapanteles dignus (muesebeck; hymenoptera: braconidae) is an american endoparasitoid that attacks the south american tomato leafminer tuta absoluta (meyrick; lepidoptera: gelechiidae). the interaction between p. dignus and t. absoluta in tomato (solanum lycopersicum l.; solanales: solanaceae) crops has demonstrated that this enemy exhibits some desirable ecological traits as an effective biological control agent of this pest. with the aim of extending the use of p. dignus to other solanaceou ... | 2017 | 29294007 |
| volatile-mediated attraction of greenhouse whitefly trialeurodes vaporariorum to tomato and eggplant. | the behavior of the greenhouse whitefly, trialeurodes vaporariorum westwood (hemiptera: aleyrodidae), is known to be affected by plant volatile cues, but its attraction or repellent to specific volatile cues has not been deeply studied yet. therefore, the aim of our study was to identify the most attractive plant among cultivars of tomato (solanum lycopersicum) and eggplant (solanum melongena) to evaluate the volatiles of plants to identify the chemical compound(s) that attract t. vaporariorum. ... | 2017 | 28775733 |
| sulphur alters chromium (vi) toxicity in solanum melongena seedlings: role of sulphur assimilation and sulphur-containing antioxidants. | the present study investigates modulation in hexavalent chromium [cr(vi) 25 μm] toxicity by sulphur (s; 0.5, 1.0 and 1.5 mm s as low (ls), medium (ms) and high sulphur (hs), respectively) in solanum melongena (eggplant) seedlings. biomass accumulation (fresh and dry weights), photosynthetic pigments, photosynthetic oxygen evolution and s content were declined by cr(vi) toxicity. furthermore, fluorescence characteristics (jip-test) were also affected by cr(vi), but cr(vi) toxicity on photosystem ... | 2017 | 28088020 |
| an "aubergine" in the heart: huge native mitral valve endocarditis caused by streptococcus agalactiae. | 2017 | 28815399 | |
| detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in saudi arabia. | a total of 1368 symptomatic plant samples showing different virus-like symptoms such as mottling, chlorosis, mosaic, yellow mosaic, vein clearing and stunting were collected from alfalfa, weed and cultivated plant species growing in vicinity of alfalfa fields in five principal regions of alfalfa production in saudi arabia. das-elisa test indicated occurrence of 11 different viruses in these samples, 10 of which were detected for the first time in saudi arabia. eighty percent of the alfalfa sampl ... | 2017 | 28855829 |
| in silico analysis reveals widespread presence of three gene families, mapk, mapkk and mapkkk, of the mapk cascade from crop plants of solanaceae in comparison to the distantly-related syntenic species from rubiaceae, coffee. | mitogen-activated protein kinases (mapks) are an important family of genes which play roles in vital plant processes, and they also help in coping against various kinds of environmental stresses including abiotic as well as biotic factors. the advancement of genomics calls for the annotation, identification, and detailed processing of the essential gene families in plants in order to provide insights into the importance of their central roles as well as for providing the basis for making their g ... | 2017 | 28603666 |
| assessing the pathogenic ability of ralstonia pseudosolanacearum (ralstonia solanacearum phylotype i) from ornamental rosa spp. plants. | ralstonia pseudosolanacearum (ralstonia solanacearum phylotype i) isolates found in stunted, yellowing, and wilted ornamental rose (rosa spp.) were assessed for their pathogenic ability in two rose cultivars (cv. "armando" and cv. "red naomi") and in four solanaceous crops: tomato (solanum lycopersicum cv. "money maker"), tobacco (nicotiana tabacum cv. "white burley"), eggplant (solanum melongena cv. "black beauty") and sweet pepper (capsicum annum cv. "yolo wonder"). significant differences wer ... | 2017 | 29163615 |
| is root catalase a bifunctional catalase-peroxidase? | plant catalases exhibit spatial and temporal distribution of their activity. moreover, except from the typical monofunctional catalase, a bifunctional catalase-peroxidase has been reported. the aim of this study was to investigate whether the leaf and root catalases from six different plant species (lactuca sativa, cichorium endivia, apium graveolens, petroselinum crispum, lycopersicon esculentum, and solanum melongena) correspond to the monofunctional or the bifunctional type based on their sen ... | 2017 | 28587060 |
| dynamics of calcium during in vitro microspore embryogenesis and in vivo microspore development in brassica napus and solanum melongena. | calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program. however, there are no direct clues about calcium levels during microspore embryogenesis, an experimental process that combines a developmental switch toward embryogenesis and the simultaneous application of different stressing factors. in this work, we used fluoforte, a calcium-specific fluorescent vital dye, to track by confocal microsc ... | 2017 | 28736567 |
| evaluation of the biocontrol potential of purpureocillium lilacinum qlp12 against verticillium dahliae in eggplant. | a fungus with broad spectrum antifungal activity was isolated from the soil in qinling mountain, shaanxi province, in china. the fungus was identified as purpureocillium lilacinum based on its rdna gene analysis. the strain, coded as qlp12, showed high inhibition activity on fungal mycelium growth in vitro, especially to mucor piriformis, trichothecium roseum, rhizoctonia solani, and verticillium dahliae, and its potential for biocontrol efficacy of eggplant. verticillium wilt disease caused by ... | 2017 | 28303252 |
| draft genome sequence of an isolate of fusarium oxysporum f. sp. melongenae, the causal agent of fusarium wilt of eggplant. | here, we present the genome sequence of an isolate (14004) of fusarium oxysporum f. sp. melongenae, an eggplant pathogen. the final assembly consists of 1,631 scaffolds with 53,986,354 bp (g+c content, 46.4%) and 16,485 predicted genes. | 2017 | 28209821 |
| synthesis and fungicidal activity of tryptophan analogues - the unexpected calycanthaceous alkaloid derivatives. | a series of 21 n-protected tryptophan derivatives were synthesised from tryptophan in good yields. their structures were characterised by ir, (1)h nmr, (13)c nmr, dept (90° and 135°) and ms analysis. the synthesised compounds were evaluated against a wide variety of plant pathogen fungi. compounds a19 and a21 displayed activity against fusarium oxysporum (f. oxysporum), and compound a21 showed high activity against f. oxysporum and eggplant verticillium, with ec50 values of 58.27 and 77.39 μg ml ... | 2017 | 27653454 |
| nutrient-rich versus nutrient-poor foods for depressed patients based on iranian traditional medicine resources. | considering the positive effects of certain nutrients on depression, increasingly prevalent in the contemporary societies, we investigated the nutritional content of prescribed and prohibited foodstuffs for depressed patients in iranian traditional medicine resources. | 2017 | 26445711 |
| suppressive properties of extracts from japanese edible plants regarding nitric oxide generation. | acetone extracts from a total of 30 species (197 samples) of plants commonly eaten in japan were tested for their in vitro inhibitory properties against nitric oxide (no) generation in a murine macrophage cell line, raw 264.7, that had been stimulated with lipopolysaccharide in combination with interferon-g. evaluation of the effects of treatment with 100 mg/ml revealed that 6 extracts (3.1%) exerted a strong inhibitory effect (inhibition rate (ir) > or = 70%) with strong cell viability (cv> or ... | 2017 | 19537895 |
| changes in soil bacterial community structure as a result of incorporation of brassica plants compared with continuous planting eggplant and chemical disinfection in greenhouses. | greenhouse eggplant monocropping in china has contributed to the aggravation of soil-borne diseases, reductions in crop quality and yield, and the degradation of physical and chemical soil properties. crop rotation is one effective way of alleviating the problems of continuous cropping worldwide; however, few studies have reported changes in soil bacterial community structures and physical and chemical soil properties after brassica vegetables had been rotated with eggplant in greenhouses. in th ... | 2017 | 28346463 |
| kc167, a widely used drosophila cell line, contains an active primary pirna pathway. | piwi family proteins bind to small rnas known as piwi-interacting rnas (pirnas) and play essential roles in the germline by silencing transposons and by promoting germ cell specification and function. here we report that the widely used kc167 cell line, derived from drosophila melanogaster embryos, expresses pirnas that are loaded to aub and piwi. kc167 pirnas are produced by a canonical, primary pirna biogenesis pathway, from phased processing of precursor transcripts by the zuc endonuclease, a ... | 2017 | 27789612 |
| virus surveys of capsicum spp. in the republic of benin reveal the prevalence of pepper vein yellows virus and the identification of a previously uncharacterised polerovirus species. | surveys were conducted in 2014 and 2015 in southern and northern benin, respectively, to identify the viruses infecting peppers (capsicum spp.). the samples were screened by elisa for cucumber mosaic virus (cmv), pepper veinal mottle virus (pvmv), potato virus y (pvy) and tomato yellow leaf curl virus (tylcv). a generic reverse transcription pcr (rt-pcr) was used to test for the presence of poleroviruses. elisa tests confirmed the prevalence of all viruses, while the rt-pcr detected pepper vein ... | 2017 | 28220324 |
| one-step multiplex rt-qpcr detects three citrus viroids from different genera in a wide range of hosts. | a one-step multiplex reverse transcription real-time quantitative polymerase chain reaction (rt-qpcr) based on species-specific minor groove binding (mgb) probes, was developed for the simultaneous detection, identification, and quantification of three citrus viroids belonging to different genera. citrus exocortis viroid (pospiviroid), hop stunt viroid (hostuviroid), and citrus bark cracking viroid (cocadviroid) cause a variety of maladies in agriculturally significant crops. therefore, reliable ... | 2017 | 28300606 |
| overexpression of the ascorbate peroxidase gene from eggplant and sponge gourd enhances flood tolerance in transgenic arabidopsis. | previously, we found that the flood resistance of eggplant (solanum melongena) and sponge gourd (luffa cylindrica) enhanced ascorbate peroxidase (apx) activity under flooding, and consequently, both the smapx and lcapx genes were cloned. in this study, the smapx and lcapx genes were transferred under a ubiquitin promoter to arabidopsis (at) via agrobacterium tumefaciens. the expression and amount of apx and apx activities of the smapx and lcapx transgenic lines were significantly higher than tho ... | 2017 | 28150172 |
| isolation of new polar granule components in drosophila reveals p body and er associated proteins. | germ plasm, a specialized cytoplasm present at the posterior of the early drosophila embryo, is necessary and sufficient for germ cell formation. germ plasm is rich in mitochondria and contains electron dense structures called polar granules. to identify novel polar granule components we isolated proteins that associate in early embryos with vasa (vas) and tudor (tud), two known polar granule associated molecules. we identified maternal expression at 31b (me31b), eif4a, aubergine (aub) and trans ... | 2017 | 18590813 |
| rna-seq-derived identification of differential transcription in the eggplant (solanum melongena) following inoculation with bacterial wilt. | eggplant (solanum melongena) is a major vegetable crop worldwide. however, it is susceptible to bacterial wilt (bw) caused by ralstonia solanacearum, which has become an important factor limiting eggplant yield and quality. the underlying mechanism of bw remains unknown. here, rna-sequencing was used to characterize the transcriptomes of resistant (r) and susceptible (s) strains before (r0, s0) and after (r1, s1) r. solanacearum inoculation. after the removal of low-quality sequences and assembl ... | 2018 | 29104166 |
| genotyping by sequencing highlights a polygenic resistance to ralstonia pseudosolanacearum in eggplant (solanum melongena l.). | eggplant cultivation is limited by numerous diseases, including the devastating bacterial wilt (bw) caused by the ralstonia solanacearum species complex (rssc). within the rssc, ralstonia pseudosolanacearum (including phylotypes i and iii) causes severe damage to all solanaceous crops, including eggplant. therefore, the creation of cultivars resistant to r. pseudosolanacearum strains is a major goal for breeders. an intraspecific eggplant population, segregating for resistance, was created from ... | 2018 | 29370090 |
| iclip of the piwi protein aubergine in drosophila embryos. | piwi-interacting rnas (pirnas) are a class of small noncoding rnas bound to specific argonaute proteins, the piwi proteins. pirnas target mrnas by complementarity to silence them; they play an important role in the repression of transposable elements in the germ line of many species. pirnas and piwi proteins are also involved in diverse biological processes through their role in the regulation of cellular mrnas. in the drosophila embryo, they contribute to the maternal mrna decay occurring durin ... | 2018 | 29236253 |
| cclip-seq: retrieval of chimeric reads from hits-clip (clip-seq) libraries. | hits-clip (high-throughput sequencing after in vivo crosslinking and immunoprecipitation, clip-seq) libraries contain fragments of the rna sequences bound in vivo by an rna binding protein (rbp). such fragments, especially if they represent rna duplexes bound in vivo by the rbp, can occasionally be ligated together to form chimeric clip tags. chimeric clip tags from argonaute clip libraries can provide the exact base pairing profiles of small rnas with their target rna sequences, thus solving a ... | 2018 | 29030843 |
| assessment of edibility and effect of arbuscular mycorrhizal fungi on solanum melongena l. grown under heavy metal(loid) contaminated soil. | arbuscular mycorrhizal fungi (amf) aids in plant establishment at heavy metal(loid) (hm) contaminated soils, strengthening plant defense system along with promoting growth. a pot experiment was carried out to evaluate the effect of amf on eggplants grown under hm stress. further, the potential health risks of hm exposure to the humans via dietary intake of eggplant were also estimated. results showed that amf application improved growth, biomass and antioxidative defense response of plants again ... | 2018 | 29091834 |
| potential of delphinidin-3-rutinoside extracted from solanum melongena l. as promoter of osteoblastic mc3t3-e1 function and antagonist of oxidative damage. | increasing evidence suggests the potential use of natural antioxidant compounds in the prevention/treatment of osteoporosis. this study was undertaken to investigate the effects of purified delphinidin-3-rutinoside (d3r), isolated from solanum melongena l., on osteoblast viability and differentiation in basal conditions and its ability to protect mc3t3-e1 cells against oxidative damage induced by tert-butyl hydroperoxide (t-bhp). | 2018 | 29372310 |
| disentangling pectic homogalacturonan and rhamnogalacturonan-i polysaccharides: evidence for sub-populations in fruit parenchyma systems. | the matrix polysaccharides of plant cell walls are diverse and variable sets of polymers influencing cell wall, tissue and organ properties. focusing on the relatively simple parenchyma tissues of four fruits - tomato, aubergine, strawberry and apple - we have dissected cell wall matrix polysaccharide contents using sequential solubilisation and antibody-based approaches with a focus on pectic homogalacturonan (hg) and rhamnogalacturonan-i (rg-i). epitope detection in association with anion-exch ... | 2018 | 29291850 |