Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| characterization of octyl glucoside-solubilized cell membrane receptors for binding measles virus. | optimum conditions were determined for solubilizing cell membrane receptors for binding measles virus (mv). evidence for specific receptors was shown from the saturability of mv binding to intact vero cells or african green monkey erythrocytes (mrbcs). receptors, solubilized from vero cells and mrbc with 1.5% octyl glucoside, inhibited mv attachment, infectivity, and hemagglutination activities. extracts from chicken erythrocytes, which did not bind mv, were inactive in all assays. mv binding ac ... | 1989 | 2672566 |
| specific detection of circulating surface/secreted glycoproteins of viable cysticerci in taenia saginata cysticercosis. | a mouse monoclonal antibody (moab), reactive with a repetitive carbohydrate epitope on lentil-lectin adherent glycoproteins present on the surface and in the secretions of taenia saginata cysticerci, was used in the construction of a diagnostic elisa assay to detect these glycoproteins in the serum of t. saginata infected cattle. the moab was used as the trapping layer and subsequently bound glycoprotein was revealed using the same moab and biotinylated peroxidase-streptavidin complex as the dev ... | 1989 | 2674862 |
| purification and properties of a protease from developing porcine dental enamel. | a protease of molecular weight 29,000 was isolated and purified using ammonium sulphate precipitation, lentil lectin-sepharose affinity chromatography and deae-5pw ion-exchange chromatography. the protease had an unusual amino acid composition including 5% serine, 6% proline and 20% tyrosine. it was a glycoprotein containing 12-15% carbohydrate by weight. activity was optimal at 40-45 degrees c using [3h]-acetyl casein substrate and at 40-55 degrees c using [3h]-acetyl enamel protein substrate. ... | 1989 | 2684109 |
| mitogenic lectins bind to the antigen receptor on human lymphocytes. | the specificity of interactions between mitogenic and non-mitogenic lectins and disulfide-linked cell surface receptors on human lymphocytes was explored. lysates (nonidet-p40) of surface-radioiodinated tonsil lymphocytes and t lymphoblastoid cells (hpb-all) were absorbed with lectin-agarose derivatives (or bovine serum albumin, bsa-agarose) or immunoprecipitated with appropriate monoclonal antibodies (mab). lectin eluates and solubilized immunoprecipitates were analyzed by two-dimensional (nonr ... | 1989 | 2703017 |
| lectin histochemistry of mammalian brunner's glands. | lectin histochemical study was performed on twenty-eight specimens of formalin-fixed paraffin embedded tissues of proximal duodenum from human, cat, dog and rhesus (macaque) monkey to demonstrate the pattern of carbohydrate residues in submucosal glands of brunner as compared to that of the duodenal absorptive and goblet cells. ten different biotinylated lectins were used as probes, and avidin-biotin-peroxidase (abc) or avidin-gold-silver (ags) complexes were used as "visualants". brunner's glan ... | 1989 | 2715049 |
| wheat germ agglutinin, concanavalin a, and lens culinalis agglutinin block the inhibitory effect of nerve growth factor on cell-free phosphorylation of nsp100 in pc12h cells. | it has been shown that in pc12 and its subclone pc12h treatment of the cells with nerve growth factor (ngf) induces a selective decrease in the incorporation of radioactive phosphate into a 100,000-dalton protein, designated in an earlier study as nsp100, in the subsequent phosphorylation of soluble extracts from cells with (gamma-32p)atp. in the present study, we show that plant lectins, wheat germ agglutinin (wga), concanavalin a (con a), and lens culinaris agglutinin (lca), inhibit the action ... | 1989 | 2720800 |
| macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor. | human macrophage differentiation inducing factor (dif) can induce differentiation of human myeloid leukemic cells into macrophage-like cells in vitro. a procedure is described for purification of dif from serum-free human monocytic leukemia thp-1 cell-conditioned medium. the procedure included concentration of a conditioned medium by ultrafiltration, lentil lectin-sepharose affinity chromatography, and fast protein liquid chromatography using mono s and mono q. dif-a of pi 9.0 and dif-b of pi 8. ... | 1989 | 2722807 |
| characterization and solubilization of cyclo(his-pro) binding from rat liver membranes. | we have found cyclo(his-pro) binding in rat liver plasma membranes. this study focused on the characterization of solubilized binding for cyclo(his-pro) in rat liver membranes. the cyclo(his-pro) binding of liver membranes was solubilized by digitonin and octyl-glucopyranoside. the efficiency of solubilization with digitonin was greater. however, cyclo(his-pro) binding was not solubilized by triton x-100, chaps, or lubrol. digitonin-solubilized membranes showed cyclo(his-pro) binding with a high ... | 1989 | 2726776 |
| [identification of a human sperm antigen to infertile women's serum and follicular fluid with high sperm immobilizing activity]. | in our previous paper, we reported a sperm coating antigen with a molecular weight of approximately 12,000 daltons from sperm membrane fraction by using rabbit anti-seminal plasma antiserum. in this paper, we isolated a human sperm antigen with the same sperm preparation by using sera of infertile women with sperm immobilizing antibody. the isolated antigen showed the following characteristics: (1) it is a glycoprotein with an affinity with lentil lectin and its molecular weight is approximately ... | 1989 | 2746071 |
| glycosylation reduces the bioactivity of calcitonin. | the biological significance of peptide hormone glycosylation is uncertain. to examine the effect of asn-linked glycosylation on calcitonin's bioactivity we purified glycosylated calcitonin from a transplantable rat medullary thyroid carcinoma. glycosylated calcitonin constituted 2.3% of the total extracted immunoreactive calcitonin. the structure of this peptide differed from nonglycosylated calcitonin only by the oligosaccharide modification of asparagine 3. affinity of glycosylated calcitonin ... | 1989 | 2748417 |
| glycoconjugates in exfoliation syndrome. a lectin histochemical study of the ciliary body and lens. | the binding of ten biotin-coupled lectins with different carbohydrate specificities to exfoliative material and neighbouring tissues was studied in 16 formalin-fixed and paraffin-embedded human eyes. eight of the eyes were exfoliation positive while the rest were exfoliation negative. exfoliative material reacted intensely with lens culinaris (lca), canavalia ensiformis (cona) and ricinus communis (rca-i) agglutinins. positive reaction was also seen with wheat germ (wga), peanut (pna) and soybea ... | 1989 | 2763817 |
| glycoconjugates of the intestinal epithelium of the domestic fowl (gallus domesticus): a lectin histochemistry study. | a lectin histochemical study was performed on formalin-fixed paraffin-embedded tissues of duodenum, jejunum, ileum, caecum and colon from six fasted and six non-fasted 8-week-old chickens (gallus domesticus). the purpose of this study was to identify in situ the pattern of carbohydrate residues present on the luminal surface of the intestinal epithelium. ten biotinylated lectins with different sugar specificities were used as probes, and avidin-biotin-peroxidase complex (abc) was used as a 'visu ... | 1989 | 2777634 |
| induction of ige synthesis in anti-igm-activated nonatopic human b cells by recombinant interleukin-3. | the signals required to induce purified normal human b cell subpopulations into ige production were studied. pokeweed mitogen (pwm)-stimulated t cell supernatant induced ige synthesis in low-density but not high-density percoll-gradient-separated resting b cells. the pwm supernatant also enhanced (greater than 2-fold) ige synthesis by anti-igm (but not staphylococcus a cowan i)-activated high-density b cells (but not low-density b cells) and had affinity for lentil lectin. subsequent studies wer ... | 1989 | 2786506 |
| concanavalin-a, lentil, and ricin lectin affinity binding characteristics of human thyrotropin: differences in the sialylation of thyrotropin in sera of euthyroid, primary, and central hypothyroid patients. | tsh from human serum was separated into classes by serial lectin affinity chromatography using concanavalin-a (cona), lentil, and ricin lectins. tsh from 10 euthyroid subjects, 40 patients with primary hypothyroidism, and 1 patient with central hypothyroidism was studied. the patterns of cona and lentil affinity binding were similar for diverse patients; forms of tsh that bound firmly to cona also tended to bind firmly to lentil. differences in tsh-ricin binding suggested that there were differe ... | 1989 | 2793998 |
| immunochemical characterization of the suppressor factor from early human decidual cells. | an immunosuppressive factor was obtained from culture supernatants of early human decidual cells. the suppressor factor was concentrated by gel filtration in a fraction with a molecular weight between 43,000 and 67,000 daltons. it was further purified by biochemical methods. four peaks were obtained in the fraction with molecular weight between 43,000 and 67,000 daltons by anion exchange chromatography. only the second peak had immunosuppressive activity in mlr. lentil-lectin affinity chromatogr ... | 1989 | 2799918 |
| analysis of wheat germ agglutinin (wga), phaseolus vulgaris leucoagglutinin (pha-l), and lens culinaris agglutinin (lca) binding to isolated rat neocortical membrane glycoproteins and to brain tissue sections. | different lectin transport directions--either anterograde or retrograde in tracing neuronal pathways--have been attributed to different sugar specificities of the lectins. to test this hypothesis, transmembrane neocortical glycoproteins were isolated to analyze their lectin-binding properties. the lectins tested exhibited a broad binding pattern to these glycoproteins. arguing against the above-mentioned hypothesis, sugar residues of transmembranal glycoproteins probably are not exclusively resp ... | 1989 | 2819434 |
| an enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (taenia solium). | an enzyme-linked immunoelectrotransfer blot (eitb) assay was developed for immunodiagnosing human cysticercosis. the assay uses lentil-lectin, affinity-purified glycoprotein antigens. a battery of 532 serum and 46 cerebrospinal fluid (csf) samples (148 cases of parasitologically confirmed cysticercosis, 54 healthy controls, and 18 types of heterologous infections [376 cases]) were used to ascertain the assay's efficacy. all but three of the samples from cases of confirmed cysticercosis were posi ... | 1989 | 2909643 |
| [nutritional quality of the protein of the false lentil (vicia sativa ssp. abovata (ser) gaudin]. | the common vetch (vicia sativa) is a legume that grows spontaneously in the central zone of chile. it is consumed by the rural population, as lentil, and frequently it is utilized in animal feeding. the objective of the present work was to study the biological quality of its protein and protein digestibility, as well as its dietary fiber pattern. the samples studied were provided by the instituto de investigaciones agropecuarias (inia), where in its chemical composition, dietary fiber, biologica ... | 1990 | 1967029 |
| characterization of gamma-glutamyltranspeptidase from human pancreatic cancer. | to elucidate the specific changes of pancreatic gamma-glutamyl-transpeptidase (gamma-gtp) associated with malignant transformation, some properties of gamma-gtp purified from pancreatic cancer were compared with those of gamma-gtps from normal pancreas and other tissues. four of five pancreatic cancer gamma-gtps showed distinctly slower electrophoretic mobility than normal pancreatic enzymes. isoelectric points of pancreatic cancer gamma-gtps varied in each case, but they were all higher than th ... | 1990 | 1967152 |
| [structural characterization of the somatostatin receptors on rat cerebrocortical membranes]. | we characterized structurally the receptors for somatostatin in rat cerebral cortex by affinity labeling with [125i-tyr1] somatostatin. [125i-tyr1] somatostatin was cross-linked to cerebrocortical membranes using photoreactive cross-linker: n-5-azido-2-nitrobenzoyloxy-succinimide. analysis by autoradiography revealed a broad band centered at mr = 72,000 in the presence or absence of dithiothreitol. affinity labeling of and specific [125i-tyr1] somatostatin binding to cerebrocortical membranes we ... | 1990 | 1980894 |
| [lysosomes in embryonic cell differentiation]. | lysosomes as a modeling factor of embryonal morphogenesis occupy an important place in the systemic structural and ultrastructural studies on prenatal development of man. human embryos at the age of 6 to 10 weeks of embryonal development, obtained after interruption of normal pregnancy by the method vacuum excochleation according to andreev. lysosomes were investigated in cells of groups, chosen in advance, from organs with various origin and degree of cellular differentiation: neurones of the s ... | 1990 | 2098255 |
| precocious loss of cortical granules during mouse oocyte meiotic maturation and correlation with an egg-induced modification of the zona pellucida. | fertilization results in cortical granule exocytosis, which is thought to be involved in modifications of the zona pellucida that constitute the zona pellucida block to polyspermy. a previous report demonstrated that a decrease in the number of lens culinaris agglutinin-staining granules, which are likely to be cortical granules, occurred during in vivo mouse oocyte maturation with arrest at metaphase ii, as well as the formation of a cortical granule-free domain in the area of the metaphase ii ... | 1990 | 2104813 |
| purification, calcium binding properties, and ultrastructural localization of the 53,000- and 160,000 (sarcalumenin)-dalton glycoproteins of the sarcoplasmic reticulum. | the 53-kda glycoprotein and sarcalumenin (160-kda glycoprotein) were extracted from rabbit skeletal muscle sarcoplasmic reticulum with egta and purified by fractionation on deae-sephadex a-25 and lentil lectin-sepharose 4b. sarcalumenin was shown to bind up to 400 nmol of ca2+/mg of protein at ph 7.5, which is equivalent to binding of approximately 35 mol of ca2+/mol of protein. the apparent dissociation constant was 300 microm in the presence of 20 mm kcl and 600 microm in 150 mm kcl. the 53-kd ... | 1990 | 2112542 |
| heterogeneity of latent transforming growth factor-beta isolated from bone matrix proteins. | transforming growth factor-beta (tgf beta) is a family of 25-kda peptides that appear to be important regulators of cell proliferation, differentiation, and differentiated function in many tissues. tgf beta is present in platelets and serum and is released by cultured cells as several distinct large mol wt complexes of tgf beta with other proteins. these complexes are biologically inactive and are generically called latent tgf beta (l-tgf beta). large quantities of tgf beta are present in bone m ... | 1990 | 2137079 |
| a cerebellar purkinje cell marker p400 protein is an inositol 1,4,5-trisphosphate (insp3) receptor protein. purification and characterization of insp3 receptor complex. | p400 protein is a 250 kd glycoprotein, characteristic of the cerebellum, which is accumulated at the endoplasmic reticulum, at the plasma membrane and at the post-synaptic density of purkinje cells. in this study, we purified inositol 1,4,5-trisphosphate (insp3) receptor from mouse cerebellum and examined the possibility that p400 protein is identical with cerebellar insp3 receptor protein. insp3 receptor was solubilized with triton x-100 from a post-nuclear fraction of ddy mouse cerebellum and ... | 1990 | 2153079 |
| increased in-vivo insulin sensitivity but normal liver insulin receptor kinase activity in dwarf chickens. | the effects of the recessive and sex-linked dw gene on insulin sensitivity and liver insulin receptors were compared in normal (dw-dw) and dwarf (dw-dw) brother or half-brother chickens. at 3.5 weeks of age, following an overnight fast, exogenous insulin (0-6.9 nmol/kg body weight) was slightly but significantly more hypoglycaemic in dwarf chickens. at 4 weeks of age, following an oral glucose load (2 g/kg), glucose tolerance was the same in both genotypes, whereas plasma insulin levels were gre ... | 1990 | 2166126 |
| adsorptive and fluid phase endocytosis by cultured rabbit corneal endothelium. | in addition to maintaining corneal transparency via a pump-leak mechanism, the corneal endothelium plays an active role in the transport of certain proteins to supply nutrients for the stroma and to remove metabolites. in order to investigate transcellular transport mechanisms across the endothelium, we exposed cultured rabbit corneal endothelial cells to tracers that are commonly used to describe various forms of endocytosis. the cells were incubated on their apical surfaces in solutions contai ... | 1990 | 2189689 |
| clear cell carcinoma of the human ovary synthesizes and secretes a transferrin with microheterogeneity of lectin affinity. | human ovarian clear cell carcinoma cell line (transferrin (tf)-non-producer), hac 2, cells were adapted to grow in chemically defined synthetic medium when the cells were cultured with medium containing 10 micrograms/ml of insulin at least for 6 months. they synthesized and secreted constantly the 80 kda protein immunologically similar to human serum tf (15 +/- 12 ng/ml/10(7) cells/3 days). by sensitive lectin-affinity electrophoresis followed by antibody-affinity blotting technique, a concanava ... | 1990 | 2226780 |
| rat brain n-acetylated alpha-linked acidic dipeptidase activity. purification and immunologic characterization. | n-acetylated alpha-linked acidic dipeptidase (naala dipeptidase) is a membrane-bound metallopeptidase that cleaves glutamate from the endogenous neuropeptide n-acetyl-l-aspartyl-l-glutamate. in this report, we have solubilized naala dipeptidase activity from synaptosomal membranes with triton x-100 and purified it to apparent homogeneity by sequential column chromatography on deae-sepharose, cm-sepharose, and lentil lectin-sepharose. this procedure resulted in a 720-fold purification with 1.6% y ... | 1990 | 2250024 |
| ontogenesis of the secretory epithelium of the bovine subcommissural organ. a histofluorescence study using lectins and monoclonal antibodies. | a spatio-temporal analysis of the differentiation of a group of specialized (secretory) ependymal cells in the subcommissural organ (sco) of the brain was undertaken in the bovine using a monoclonal antibody (c1b8a8) which is specific of the secretory process in this organ. in addition, lectins (concanavalin agglutinin (con a), lens culinaris agglutinin (lca), wheat germ agglutinin (wga), and phaseolus vulgaris agglutinin (pha] were used to analyse the maturation of the carbohydrate moieties of ... | 1990 | 2253320 |
| high molecular weight soluble neutral maltase-glucoamylases in the intestine of the suckling rat. | previous work from our laboratory has shown that the intestine of the suckling rat, unlike adult rat intestine, contains abundant quantities of at least two soluble neutral maltase-glucoamylases. these enzymes are related antigenically to membrane-bound maltase-glucoamylase, which predominates in adult intestine, but are either more easily solubilized or occupy a different cellular locus. to study the soluble enzymes further, we attempted their isolation from the intestine of 11-day-old suckling ... | 1990 | 2257117 |
| identification of the surface polypeptide antigens of the infective larvae of onchocerca volvulus. | the surface polypeptide antigens of third-stage infective larvae (l3) and adult males of onchocerca volvulus have been compared after iodogen-catalysed radioiodination, separation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in beta-mercaptoethanol followed by autoradiography. l3 surfaces contained polypeptides with apparent molecular weights of 14, 18.5, 26, 34, 51, 68 and 130 kda, whereas the adults contained 14, 19, 26, 34, 37.5 and 68 kda components. by immunoprecipitation w ... | 1990 | 2278076 |
| recombinant carboxyl-terminal fibrin-binding domain of human fibronectin expressed in mouse l cells. | the carboxyl-terminal fibrin-binding domain, fib2, of human fibronectin was expressed in mouse l cells as a fusion protein with the signal sequence of human protein c inhibitor. the recombinant fib2 (rfib2) protein synthesized by transfected cells retained the ability to form dimers with each other or with mouse fibronectin subunits and was secreted to the medium after extensive glycosylation. only a small fraction of the secreted protein was incorporated into the pericellular matrix. interestin ... | 1990 | 2294110 |
| secretory glycoconjugates of a mucin-synthesizing human colonic adenocarcinoma cell line. analysis using double labeling with lectins. | lectins were used to characterize mucin glycoproteins and other secretory glycoconjugates synthesized by a human colon adenocarcinoma-derived cell line which expresses a goblet cell phenotype. despite being clonally derived, ht29-18n2 (n2) cells, like normal goblet cells in situ were heterogeneous in their glycosylation of mucin. only wheat-germ agglutinin, which recognizes n-acetylglucosamine and sialic acid residues, and succinylated wheatgerm agglutinin, which binds n-acetylglucosamine, stain ... | 1990 | 2312359 |
| membrane-bound and water-soluble nonclassical class i mhc antigens on rat placenta. | we have used mouse monoclonal antibodies to different determinants on rat class i major histocompatibility complex (mhc) antigens in order to identify water-soluble and membrane-bound nonclassical (i. e., non-rt1.a) class i mhc antigens on the spongiotrophoblast and labyrinthine trophoblast of rat placenta. initial immunohistological studies with monoclonal antibodies reacting with a determinant restricted to classical (rt1.a) rat class i antigens confirmed the presence of these antigens on spon ... | 1990 | 2318515 |
| duck lymphocytes--iii. transformation responses to some common mitogens. | the lymphocyte transformation (lt) test was performed using duck blood lymphocytes stimulated with phytohaemagglutinin (pha), concanavalin a (con a), lentil lectin (lc), roman snail lectin (hp), peanut agglutinin (pna), bandeiraea simplicifolia seed lectin (bss), wheat germ agglutinin (wga), horseshoe crab lectin (hsc), pokeweed mitogen (pwm) and e. coli lipopolysaccharide (lps). cells were cultured in microtitre trays, at 41.6 degrees c, 8 x 10(5) cells in 200 microliters medium (= 4 x 10(6) ce ... | 1990 | 2323180 |
| neurothelin: an inducible cell surface glycoprotein of blood-brain barrier-specific endothelial cells and distinct neurons. | the blood-brain barrier is characterized by still poorly understood barrier and transport functions performed by specialized endothelial cells. hybridoma technology has been used to identify a protein termed neurothelin that is specific for these endothelial cells. neurothelin is defined by the species-specific mouse mab 1w5 raised against lentil-lectin-binding proteins of neural tissue from embryonic chick. in the posthatch chick, neurothelin expression is found on endothelial cells within the ... | 1990 | 2324198 |
| lectin-resistant variants of mouse lewis lung carcinoma cells. ii. altered glycosylation of membrane glycoproteins. | lectin-resistant variants of mouse lewis lung carcinoma ll2 cell line, selected with wheat germ agglutinin (wgar), ricinus communis agglutinin ii (rca iir) and aleuria aurantia agglutinin (aaar) were studied. total cellular glycopeptides of the parent ll2 line and of the five lectin-resistant variants were analyzed by gel filtration and affinity chromatography on immobilized concanavalin a and lens culinaris agglutinin. the results revealed that low-metastatic wgar and rca iir variants possessed ... | 1990 | 2328549 |
| lectin staining of saccharides in the normal and hypothyroid developing organ of corti. | lectin staining has been used to detect mono- and oligosaccharides in normal and hypothyroid developing organs of corti in the rat. eight developmental stages were studied (1, 5, 8, 10, 15, 20, 50 and 60 days after birth). congenital hypothyroidism was induced by oral administration of propylthyouracil to pregnant rats. labelling of the tectorial membrane with 3 lectins, ulex europaeus agglutinin-i (uea-i), lens culinaris agglutinin (lca) and ricinus communis agglutinin-i (rca-i) showed no signi ... | 1990 | 2331782 |
| structure of asparagine-linked oligosaccharides on human and rabbit testosterone-binding globulin. | we have previously demonstrated, using two-dimensional polyacrylamide gel electrophoresis, that much of the microheterogeneity of human (h) and rabbit (rb) testosterone-binding globulin (tebg) is due to differential glycosylation of a single protomer. since glycosylation has been shown to be a physiologically important modification of proteins, we have examined the structure of the oligosaccharide chains attached to htebg and rbtebg to facilitate future studies on the mechanisms of action of the ... | 1990 | 2340332 |
| stimulation by concanavalin a of cartilage-matrix proteoglycan synthesis in chondrocyte cultures. | the effect of concanavalin a on proteoglycan synthesis by rabbit costal and articular chondrocytes was examined. chondrocytes were seeded at low density and grown to confluency in medium supplemented with 10% fetal bovine serum, and then the serum concentration was reduced to 0.3%. at the low serum concentration, chondrocytes adopted a fibroblastic morphology. addition of concanavalin a to the culture medium induced a morphologic alteration of the fibroblastic cells to spherical chondrocytes and ... | 1990 | 2351653 |
| effect of pea and lentil lectins on in vitro absorption of nutrients. | in vitro absorption of nutrients like glucose, leucine, protein hydrolysate and ca2+ by ligated loops of small intestine was significantly affected in presence of lectins from peas and lentils. except for sucrose, all other nutrients showed significant decrease in their absorption in presence of lectins. lentil lectins had a greater inhibitory effect than pea lectins. | 1990 | 2365412 |
| in vitro effect of lectins from peas (pisum sativum) and lentils (lens culinaris) on lipid peroxidation and superoxide dismutase activity in normal and vitamin b6 deficient albino rats. | 1990 | 2365414 | |
| analysis of the oligosaccharides on rat androgen-binding protein using serial lectin chromatography. | the microheterogeneity seen when rat androgen-binding protein (rabp) is analyzed by two-dimensional polyacrylamide gel electrophoresis is attributable, at least in part, to the differential glycosylation of a single promoter. further insight into the chemical nature of the oligosaccharide units on rabp was obtained by serial lectin chromatography. when rabp was chromatographed on immobilized concanavalin a (con-a), it was fractionated into three classes: (1) one that did not bind to the lectin ( ... | 1990 | 2378933 |
| inhibition of fibrin(ogen) binding to stimulated platelets by a monoclonal antibody specific for a conformational determinant of gpiiia. | we have purified the integrin gpiib:iiia from the membrane fraction of human blood platelets by lentil lectin affinity chromatography followed by gel filtration chromatography. with purified gpiib:iiia as an antigen, we have produced monoclonal antibody cs-1, which immunoblotting demonstrates to be specific for native gpiiia; disulfide bond reduction of gpiiia resulted in loss of immunoreactivity. radiolabelled ligand binding studies revealed that cs-1 recognized approximately 55,000 sites per p ... | 1990 | 2385828 |
| heterogeneity in lectin-binding characteristics of human lymphokine-activated killer cells. | the binding of various lectins to human lymphokine-activated killer (lak) cells was investigated. human peripheral blood lymphocytes were cultured for 3 days with recombinant interleukin-2 (ril-2), and then stained with 20 kinds of lectins. there were four types of lectin-binding patterns: (a) negative staining; (b) weakly positive staining; (c) strongly positive staining; and (d) two populations, one weakly and one strongly positive staining. among the 20 kinds of lectins, both lens culinaris a ... | 1990 | 2395003 |
| carbohydrate composition of the alpha-subunit of human choriogonadotropin (hcg alpha) and the free alpha molecules produced in pregnancy: most free alpha and some combined hcg alpha molecules are fucosylated. | the carbohydrate compositions of pregnancy-derived hcg alpha (dissociated from intact hcg) and free alpha-subunit were analyzed using a combination of chemical analysis, lectin affinity chromatography, and glycosidase sensitivity. for direct compositional analysis, parallel samples were hydrolyzed in trifluoroacetic acid and analyzed for sialic acid and neutral sugars without prior derivatization. separation of the monosaccharides was achieved by hplc on a dionex carbopac column eluted at high p ... | 1990 | 1693562 |
| novel and sensitive enzyme immunoassay (immune-complex-transfer enzyme immunoassay) for alpha-fetoprotein from hepatocellular carcinoma. | alpha-fetoprotein in sera from patients with hepatocellular carcinoma was fractionated into three peaks by affinity chromatography on a column of lens culinaris agglutinin-sepharose 4b. one peak (the first peak), which passed through the column without adsorption, was found in both healthy subjects and patients with liver cirrhosis and hepatocellular carcinoma. the second and third peaks were reactive with l. culinaris agglutinin and found only in patients with hepatocellular carcinoma. for alph ... | 1990 | 1693671 |
| lectin binding ability of b-chronic lymphocytic leukaemia cells. | the binding of five fluorescein-labelled lectins: peanut agglutinin (pna), lentil agglutinin (len), soybean agglutinin (sba), wheat germ agglutinin (wga) and asparagus pea agglutinin (asp) to human b-cell chronic lymphocytic leukaemia (b-cll) and b lymphocytes of normal donors was studied. the specificity of the fluorescence was demonstrated by inhibition with appropriate saccharides. the proportion of b cells was estimated using anti-b cell monoclonal antibody. both leukaemic and normal b cells ... | 1990 | 1695180 |
| lipophosphoglycan and secreted acid phosphatase of leishmania tropica share species-specific epitopes. | several species-specific monoclonal antibodies (t11, t13-t15) which only react with leishmania tropica, recognize phosphorlated carbohydrate epitopes on lipophosphoglycan and the structurally related molecule, phosphoglycan, which is shed by promastigotes into spent culture medium. during immunoaffinity isolation of [32p]orthophosphate-labeled phosphoglycan on monoclonal antibody t15 conjugated to sepharose 4b, a high-mr component (approx. 200,000) was co-purified. the latter material is metabol ... | 1990 | 1697935 |
| organochlorine pesticide residues in different indian cereals, pulses, spices, vegetables, fruits, milk, butter, deshi ghee, and edible oils. | a total of 244 samples of cereals (wheat flour, rice, and maize), pulses (arhar, moong, gram, lentil, and black gram), spices (turmeric, chili, coriander, and black pepper), vegetables (potato, onion, spinach, cabbage, brinjal, and tomato), fruits (mango, guava, apple, and grape), milk, butter, deshi ghee, and edible oils (vegetable, mustard, groundnut, and sesame) collected from different cities of northern province (utter pradesh) were analyzed by gas liquid chromatography for the presence of ... | 1990 | 1698760 |
| lectin histochemical characteristics of the canine female mammary gland. | twelve biotinylated lectins and an avidin-biotin-peroxidase method were used to detect and localize specific carbohydrate residues on formalin-fixed, paraffin-embedded female canine mammary gland sections. histologic sections from 3 lactating and 7 nonlactating mixed-breed dogs (age 5.6 +/- 0.35 years) were incubated with arachis hypogea agglutinin (peanut agglutinin; pna), concanavalia ensiformis agglutinin (cona), dolichos biflorus agglutinin (dba), glycine max agglutinin (sba), griffonia simp ... | 1990 | 1700652 |
| interactions between lectins and acetylcholinesterase from the sarcotubular system of skeletal muscle. | the oligosaccharide chains attached to the multiple forms of acetylcholinesterase (ache) in a fraction enriched in membranes of the sarcoplasmic reticulum and t-tubules of rabbit skeletal muscle were studied using lectins. by sequential extraction of the membranes with triton x-100, two preparations of soluble enzyme were obtained, s(1) and s(2). monomeric, tetrameric and asymmetric forms of ache are present, and all of these bind to concanavalin a, yielding heavy aggregates with no loss of enzy ... | 1990 | 20504600 |
| glycoprotein storage in gaucher disease: lectin histochemistry and biochemical studies. | lectin histochemical studies were performed on formalin-fixed, frozen, and paraffin-embedded tissue sections from 19 patients with glucosylceramide lipidosis (i.e., gaucher disease). eleven different lectins were used to identify the specific carbohydrate residues in the undegraded stored compounds in the cytoplasm of gaucher cells. in all cases studied, gaucher cells stained with concanavalia ensiformis agglutinin, datura stramonium agglutinin, lens culinaris, ricinus communis agglutinin-i, and ... | 1990 | 2395332 |
| characterization of a cdna encoding lens culinaris glycolate oxidase and developmental expression of glycolate oxidase mrna in cotyledons and leaves. | mrna obtained from green leaves of lentil (lens culinaris) was used to construct a cdna library in phage lambdagt11. the cdna library was screened with antibodies raised against lentil glycolate oxidase and catalase. clone cl 1 containing the full-length sequence complementary to glycolate oxidase mrna was characterized and sequenced. in addition, a 800-base pair catalase cdna clone was sequenced. to prove the correlation of cdna insert in cl 1 with glycolate oxidase, the cdna was transcribed in ... | 1990 | 16667816 |
| n-linked oligosaccharides of the murine transferrin receptor from a plasmacytoma cell line. comparison with total cellular n-glycans. | the n-linked oligosaccharides synthesised by the murine plasmacytoma cell line ns-1 have been analysed by lectin affinity chromatography on columns of immobilised concanavalin a (con a), lens culinaris (lentil), ricinus communis agglutinin (rca) and leuko-phytohemagglutinin (l-pha). the majority of complex n-glycans in this transformed cell line were branched structures with only a low level of biantennary complex chains detected. the analysis showed the major complex n-glycan fraction consisted ... | 1990 | 2223825 |
| characterization of responses to temperature and photoperiod for time to flowering in a world lentil collection. | the times from sowing to first flowering (f) of 231 accessions of lentil (lens culinaris medik.), comprising germ plasm from eight countries and breeding lines from icarda in syria, were recorded in four glasshouse environments; two photoperiods (16 and 13 h/day) combined with warmer (24°/13°c) and cooler (18°/9°c) day/night temperatures. the linear model 1/f=a+bt + cp (where t is mean diurnal temperature and p is photoperiod) provided an average fit over the 231 accessions of r (2)=0.852. since ... | 1990 | 24220895 |
| salt-soluble lectins of corn grain. | lectins extracted from corn (zea mays l.) kernel with tris-hcl buffer ph 7.5 were isolated from the crude extract by affinity chromatography on sepharose 6b-n-acetyl-d-galactosamine and sepharose 6b-methylalpha-d-mannoside, and also by lectin affinity chromatography using concanavalin a and lens culinaris lectin as ligands. according to preferential monosaccharide specificity, salt-soluble lectins of corn seed comprise at least two distinct types: n-acetyl-d-galactosamine-interactive and mannose ... | 1990 | 16667671 |
| development of cortical vesicles in sicyonia ingentis ova: their heterogeneity and role in elaboration of the hatching envelope. | in the marine shrimp sicyonia ingentis, ova lack cortical vesicles at spawning. previous ultrastructural studies suggested that two different populations of cortical vesicles (dense vesicles and the ring vesicles) appear within 30 min post-spawning. these vesicles undergo sequential exocytosis (exocytosis of the dense vesicles followed by exocytosis of the ring vesicles) that leads to the formation of a hatching envelope around the ovum (see pillai and clark: tissue & cell 20:941-52, 1988). in t ... | 1990 | 2346649 |
| distribution of cell surface glycoconjugates during secondary neurulation in the chick embryo. | lectin histochemistry was used to examine the expression of cell surface glycoconjugates during secondary neurulation in chick embryos. fourteen lectins were applied to serial sections of the caudal region of embryos at the various stages of tail bud development. the lectins bandeiraea simplicifolia, dolichos biflorus agglutinin, phaseolus vulgaris leukoagglutinin, soybean agglutinin, sophora japonica agglutinin, ulex europaeus agglutinin and succinylated wheat germ agglutinin (swga) showed very ... | 1990 | 2297085 |
| specific enzyme immunoassay for alpha-fetoprotein from hepatocellular carcinoma. | alpha-fetoprotein in sera from healthy subjects and patients with liver cirrhosis and hepatocellular carcinoma was fractionated into three peaks by affinity chromatography on a column of lens culinaris agglutinin-sepharose 4b. one peak (the first peak) was found in all the serum samples, but the other two peaks (the second and third peaks) were found only in patients with hepatocellular carcinoma. for alpha-fetoprotein in the second and third peaks, a specific enzyme immunoassay was developed. l ... | 1990 | 1690279 |
| time course and pattern of cortical granule breakdown in hamster eggs after sperm fusion. | we have determined the temporal relationship between sperm fusion and cortical granule breakdown in the hamster egg. sperm fusion was determined by the hoechst-transfer method (stewart-savage and bavister: dev biol 128:150-157, 1988), and cortical granules were visualized with fluorescein isothiocynate-conjugated lens culinaris agglutinin (cherr et al. j exp zool 246:81-93, 1988). by 55 min after insemination, there was an 85% reduction in the density of cortical granules (fewer than four granul ... | 1991 | 1751044 |
| allozyme and morphological variability, outcrossing rate and core collection formation in lentil germplasm. | a survey of qualitative genetic variation at 3 morphological trait loci, 17 isozyme loci and a putative isozyme locus (amylase) was made for 105 lentil (lens culinaris medikus) germplasm accessions from chile, greece and turkey. new alleles were found for lap-1, me-2, pgm-c, pgm-p and 6-pgd-c. the average proportion of polymorphic loci per population was 0.19, with a range of 0 to 0.42 over populations. germplasm from chile was equally variable to that from greece and turkey on the basis of indi ... | 1991 | 24202265 |
| glycosylation at the fab portion of myeloma immunoglobulin g and increased fucosylated biantennary sugar chains: structural analysis by high-performance liquid chromatography and antibody-lectin enzyme immunoassay using lens culinaris agglutinin. | an antibody-lectin enzyme immunoassay technique which had been developed for the analysis of sugar chains of alpha-fetoprotein (n. kinoshita et al., clin. chim. acta, 179: 143-152, 1989) was used for analysis of sugar chains of myeloma immunoglobulin g (igg). the igg sugar chains of four of nine patients with myeloma were found to be highly reactive to lens culinaris agglutinin as compared with those of six normal controls and 177 patients without myeloma. this reflected a high l. culinaris aggl ... | 1991 | 1933856 |
| changes in glycoconjugate expression during early chick embryo development: a lectin-binding study. | a selection of lectins was used to investigate developmentally regulated changes in the distribution of cell surface oligosaccharides during the gastrulation and neurulation stages of early chick embryo development. lectins from three specificity classes were used: glucose/mannose specificity (concanavalin a [con a], lens culinaris agglutinin [lca], pisum sativum agglutinin [psa]); n-acetylglucosamine specificity (lycopersicon esculentum agglutinin [lea], wheat germ agglutinin [wga], succinylate ... | 1991 | 1746724 |
| lichen sclerosus et atrophicus, morphea, and coexistence of both diseases. histological studies using lectins. | histological studies using three lectins, lens culinaris agglutinin, soybean agglutinin, and ulex europaeus agglutinin-i, were carried out in a case of coexistent lichen sclerosus et atrophicus and morphea, five cases of morphea, and two cases of lichen sclerosus et atrophicus. the lectin staining patterns of the formaldehyde-fixed epidermis of patients with morphea were not different from those of normal epidermis, but epidermis of patients with lichen sclerosus et atrophicus showed different s ... | 1991 | 1716433 |
| [molecular heterogeneity of alpha-fetoprotein in ovarian or extragonadal yolk sac tumor]. | the molecular heterogeneity of alpha-fetoprotein (afp) in yolk sac tumor (yst) was investigated. the study included 14 sera from yst, 78 sera from primary hepatocellular carcinoma (phc), 5 fetal yolk sac (ys) culture fluids and 4 fetal liver culture fluids. the microheterogeneity of afp was assessed by differences in reaction with afp carbohydrate chain and lectins, and concanavalin a (con a), lens culinaris hemagglutinin (lch) and phytohemagglutinin e (pha-e) affinity crossed-line immunoelectro ... | 1991 | 1712373 |
| oxidation of spermine by an amine oxidase from lentil seedlings. | spermine is a substrate of lentil (lens culinaris) seedling amine oxidase and the oxidation products are reversible inactivators of the enzyme. the spermine is oxidized at the terminal amino groups to a dialdehyde: 2 moles of hydrogen peroxide and 2 moles of ammonia per mole of spermine are formed. the ph optimum of the enzyme with spermine is 7.9 in ti-hci buffer; the k(m) value is 4.4.10(-4) molar, similar to that found with other substrates (putrescine and spermidine). | 1991 | 16668008 |
| strategies for the purification of p-glycoprotein from multidrug-resistant chinese hamster ovary cells. | p-glycoprotein, a hydrophobic 170-kda integral protein overexpressed in the plasma membrane of multidrug-resistant cells, is proposed to function as an atp-dependent drug efflux pump. plasma membrane preparations highly enriched in p-glycoprotein were isolated from multidrug-resistant cells by discontinuous sucrose gradient and ca2+ precipitation methods. several strategies were used for p-glycoprotein purification, with the goal being to achieve both good yields and purity, while keeping experi ... | 1991 | 1687982 |
| studies on the oligosaccharide heterogeneity of the isoelectric forms of the lower molecular weight acid phosphatase of frog liver. | 1. the lower molecular weight, heterogeneous acid phosphatase (acpase) from the frog liver (rana esculenta) containing acpase i, ii, iii and iv was separated into enzymatically active components by isoelectric focusing in an immobilized ph gradient. 2. the blotted enzyme bands were characterized by their different binding patterns obtained with the lectins concanavalin a, wheat germ agglutinin (wga), lens culinaris hemagglutinin (lch) and peanut agglutinin (pna). 3. in situ neuraminidase treatme ... | 1991 | 2015951 |
| an investigation on the quinoprotein nature of some fungal and plant oxidoreductases. | the presence of pyrroloquinoline quinone (pqq) as the organic cofactor of dactylium dendroides galactose oxidase and lentil (lens culinaris) seedling amine oxidase, purported pqq-containing oxidoreductases (van der meer, r. a., jongejan, j. a., and duine, j. a. (1989) j. biol. chem. 264, 7792-7794; citro, g., verdina, a., galati, r., floris, g., sabatini, s., and finazzi-argo', a. (1989) febs lett. 247, 201-204), was reinvestigated using the nitro blue tetrazolium redoxcycling method (paz, m. a. ... | 1991 | 1657943 |
| crown gall transformation of lentil (lens culinaris medik.) with virulent strains of agrobacterium tumefaciens. | four diverse strains of agrobacterium tumefaciens (c58, ach5, gv3111, and a281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (lens culinaris medik. cultivar laird) in vivo, and on excised shoot apices in vitro. gv3111 and ach5 produced the largest and heaviest tumors in vivo, while a281 produced the heaviest tumors in vitro. tumor formation and opine production are indicative of plant cell transformation and tumors produced appropriate opines: nopaline (c58), ... | 1991 | 24221280 |
| effect of processing on some chemical and nutritional characteristics of pre-cooked and dehydrated legumes. | the effect of processing on chemical composition and protein quality of three legumes was studied. the species analyzed were beans (phaseolus vulgaris), tórtola and coscorrón varieties; lentils (lens esculenta), laird variety; and chick peas (cicer arietinum), california-inia variety. the legumes were soaked in a solution of na2 edta at 0.03% for 16 h and cooked for a predetermined period for each species. they were dried in a tray drier with cross currents of air flowing at a speed of 10 m/min ... | 1991 | 1656424 |
| [microheterogeneity of alpha-fetoprotein in the amniotic fluid--developmental changes in the molecular structure of carbohydrate chain]. | by means of lectin affinity crossed-line immunoelectrophoresis, microheterogeneity of alpha-fetoprotein (afp) was studied in 41 amniotic fluid samples between 41 to 287 days of gestation. microheterogeneity was assessed by the structural differences in the carbohydrate chain identified by the specific binding between oligosaccharide molecule(s) and certain lectins such as concanavalin a, lens culinaris hemagglutinin, phytohemagglutinin e or ricinus communis agglutinin i. it was found that four c ... | 1991 | 1705276 |
| characterization of the carbohydrate chain on the substance p receptor in the rat brain cortex: effect of lectins on [3h]substance p binding. | the characteristics of the carbohydrate chain on the rat cerebral cortical substance p (sp) receptor were studied. we examined the effects of pretreatment with three lectins (concanavalin a, wheat germ agglutinin, lens culinaris agglutinin) on the [3h]sp binding activities. each lectin can bind to the specific carbohydrate chain. among these lectins, only concanavalin a inhibited specific [3h]sp binding by reducing the affinity of the binding sites. the inhibitory action of concanavalin a was do ... | 1991 | 1705959 |
| purification and characterisation of a breast-cancer-associated glycoprotein not expressed in normal breast and identified by monoclonal antibody 83d4. | monoclonal antibody (mab) 83d4 was generated using formol-fixed paraffin-embedded human breast carcinoma tissue as the immunogen. previous studies demonstrated that it was reactive with breast carcinoma tissues, but not with normal breast. the antigen identified by mab 83d4 was detected, using elisa, in mcf7 breast carcinoma cell line membrane extracts, in primary breast and colon carcinoma tissue extracts and in pleural effusion fluid from patients with metastatic breast cancer. no reactivity w ... | 1991 | 1706194 |
| carbohydrate-binding specificity of tetracarpidium conophorum lectin. | the carbohydrate-binding specificity of a novel plant lectin isolated from the seeds of tetracarpidium conophorum (nigerian walnut) has been studied by quantitative hapten inhibition assays and by determining the behavior of a number of oligosaccharides and glycopeptides on lectin-sepharose affinity columns. the tetracarpidium lectin shows preference for simple, unbranched oligosaccharides containing a terminal gal beta 1----4glnac sequence over a gal beta 1----3glcnac sequence and substitution ... | 1991 | 1711034 |
| autoantibodies to glycoprotein antigens mediate subacute demyelinating encephalomyelitis in the lewis rat. | serum autoantibodies were induced in lewis rats by immunization with a mixture of lentil lectin-binding glycoproteins isolated from bovine brain myelin. intraperitoneal administration of 2-10 million syngeneic myelin basic protein-activated spleen cells to these rats led within 4-5 days to paralysis which, in most cases, persisted for several weeks. the major neuropathological features of the disease were numerous macrophages in both brain and spinal cord and large areas of demyelination, genera ... | 1991 | 1712364 |
| glycoprotein changes in the rat sperm plasma membrane during maturation in the epididymis. | to investigate surface glycoprotein changes during post-testicular maturation, plasma membranes were isolated from proximal caput, distal caput, and cauda epididymal rat spermatozoa. membrane glycoproteins were identified on western blots of sds-page fractionated samples using biotinylated lectins and vecta-stain reagents; these were compared to glycoproteins present in cauda epididymal luminal fluid. lens culinaris agglutinin, pisum sativum agglutinin, peanut agglutinin, wheat germ agglutinin, ... | 1991 | 1716114 |
| counterflow affinity isotachophoresis on cellulose acetate membranes. | counterflow isotachophoresis on cellulose acetate membranes of human alpha-fetoprotein (afp) was performed with concanavalin a, lentil lectin, and castor bean lectin driven by electroendosmotic counterflow. this counterflow caused a uniform stream of lectin to migrate towards the cathode against afp with carrier ampholytes in steady-state position. retardation of microheterogeneity forms bound to lectins was observed, giving results comparable to standard crossed affinity immunoelectrophoresis. ... | 1991 | 1716198 |
| lectin binding in skeletal muscle. evaluation of alkaline phosphatase conjugated avidin staining procedures. | cryostat sections from rat gracilis muscles were incubated with different biotinylated lectins: con a (concanavilin a), wga (wheat germ agglutinin), sba (soybean agglutinin), gs i and gs ii (griffonia simplicifolia agglutinin), lca (lens culinaris agglutinin), pna (peanut agglutinin) and psa (pisum sativum agglutinin). the sections were subsequently treated with alkaline phosphatase conjugated avidin. the lectin binding sites were visualized after incubation in substrate media containing: (1) 5- ... | 1991 | 1717410 |
| autosomal recessive polycystic kidney in rats. | we evaluated the characteristics of renal lesions in rat autosomal recessive polycystic kidney (arpk). in rat arpk, small cysts appeared primarily in the medulla 2 months after birth and gradually extended to the cortex, forming large cysts involving the entire layer after 8 months. by immunofluorescence microscopy, type iv collagen was more strongly stained in the epithelial basement membrane of the rat arpk than in the normal rat tubular basement membrane (tbm). electron microscopy demonstrate ... | 1991 | 1722566 |
| [value of anti-human heterogeneous lens culinaris agglutinin reactive afp monoclonal antibody in the diagnosis of primary hepatocellular carcinoma (phc)]. | in this paper, we report on the preparation and application of anti-human heterogeneous afp-r-lca monoclonal antibodies (vg5, vd12, vb5, va8, vd1). these mcabs were more sensitive and specific for afp-r-lca than the anti-afp polyclonal antibody routinely used. the twin site (a and b) sandwich elisa method so established was used to test the serum samples of 69 phc patients, 67 patients with benign liver diseases, 30 pregnant women and 30 normal controls. the results showed that this twin site sa ... | 1991 | 1723677 |
| cross-neutralizing antibodies in rabbits immunized with hiv-1 gp160 purified from simian cells infected with a recombinant vaccinia virus. | a recombinant vaccinia virus in which the transcription of the human immunodeficiency virus type 1 (bru isolate) env gene is driven by the 11k late vaccinia promoter yields about 10-fold higher amounts of gp160 env protein upon infection of monkey cells than does a recombinant in which gp160 is expressed using the 7.5k early-late promoter. the gp160 was purified from detergent lysates of infected cells by lentil lectin affinity chromatography followed by immunoaffinity chromatography, and was ob ... | 1991 | 1742074 |
| duck lymphocytes. iv. collective effects of mitogens. | duck (anas platyrhynchos) blood lymphocytes were cultured in the presence of phytohaemagglutinin (pha), concanavalin a (con a), pokeweed mitogen (pwm), lentil lectin (ll), roman snail lectin (hp), wheat germ agglutinin (wga), bandeiraea simplicifolia seed lectin (bss), peanut agglutinin (pna), horseshoe crab lectin (hsc) and bacterial lipopolysaccharide (lps). mitogens were used individually, in pairs, and in multiple combinations. some pairs resulted in synergism; transformation, assessed by up ... | 1991 | 1773860 |
| [comparative digestive and metabolic utilization of beans, lentils and chick peas in the rat]. | faba bean, lentil or chick-pea raw seeds were used as the only protein food in the rat. the balanced diet was designed to suit growth requirements. nitrogen apparent digestibility coefficient (cuda) was 72% in the faba bean, 75% in the lentil, 76% in the chick-pea groups respectively, but the cuda of some essential amino acids were much lower: 52-51-71% for methionine, 60-39-78% for cystine, 73-71-75% for valine, while arginine cuda values (87-87-82) were higher than all other aa cuda values. gr ... | 1991 | 1777055 |
| efficacy of seed-based media for the mould-yeast conversion of blastomyces dermatitidis. | the efficacy of 20 seed-based media is reported for the in vitro mould-yeast conversion of blastomyces dermatitidis, employing pharmamedia agar, peptone glucose agar, glucose agar and water agar as controls. the mould-yeast conversion varied significantly according to the culture medium, fungal strains and incubation period (p less than 0.01). garden-pea, chick-pea, cow-pea, soybean, peanut, green gram, french bean, lentil, okra and cottonseed converted all of the 7 b. dermatitidis test strains ... | 1991 | 1780002 |
| changes in cell-surface carbohydrates of trypanosoma cruzi during metacyclogenesis under chemically defined conditions. | highly purified lectins with specificities for receptor molecules containing sialic acid, n-acetylglucosamine (d-glcnac), n-acetylgalactosamine (d-galnac), galactose (d-gal), mannose-like residues (d-man) or l-fucose (l-fuc), were used to determine changes in cell-surface carbohydrates of the protozoal parasite trypanosoma cruzi during metacyclogenesis under chemically defined conditions. of the d-galnac-binding lectins, bs-i selectively agglutinated metacyclic trypomastigotes, mpl was selective ... | 1991 | 1791438 |
| [synthesis of sorbent from cross-linked starch for affinity purification of glucose(mannose)-specific lectins]. | cross-linked starch gel for the affinity chromatography of d-glucose (d-mannose)-specific lectins is suggested. in order to optimize hydrodynamic properties of gel 30% starch has been hydrolysed by hci at 70 degrees c during 60 min and then cross-linked by epichlorohydrin under alkaline conditions. every 100 g of starch require 18 ml of epichlorohydrin and 36 ml of 8 n koh. the gel obtained has been successfully used for the purification of lectins from pisum sativum l., lens culinaris l., vicia ... | 1991 | 1816693 |
| purification of beta-glucuronidase and structural assessment of the carbohydrate chains by lectin affinity immunoelectrophoresis. | the purification of rat liver beta-glucuronidase from a lysosomal fraction by methods including affinity chromatography, chromatofocusing and preparative page steps is described. molecular weights of 300,000 and 150,000 were estimated by two dimensional gradient page/immunoelectrophoresis of the lysosomal extract. isoelectrofocusing in agarose gel followed by immunoelectrophoresis in the second dimension revealed the presence of at least five maxima in the range ph 4.3-7.4. the structural assess ... | 1991 | 1841676 |
| normal number and kinase activity of insulin receptors in liver of genetically fat chickens. | genetically fat (fl) chickens exhibit an increase in liver lipogenesis and, in the fasting state, insulin sensitivity compared with genetically lean (ll) chickens. liver insulin receptor number and insulin receptor kinase (irk) activity were studied at 5 wk. in the fasting (16 h) and fed state, insulin binding to liver membranes and insulin receptor affinity (50% inhibition of tracer binding, range: 5-7 ng/ml at 4 degrees c) were very similar in fat and lean chickens. insulin degrading activity ... | 1991 | 1848286 |
| isolation and partial characterization of a soluble oncofetal antigen from murine and human amniotic fluids. | a soluble (cell-free) oncofetal antigen (ofa) was detected in murine and human amniotic fluids by immunostaining with the murine monoclonal antibody (mab 115) produced by syngeneic immunization with mid-gestational mouse fetal cells. ofa was purified from the amniotic fluids by ammonium sulfate precipitation at 30-70% saturation, followed by successive gel chromatography of the ofa-containing fraction on sephacryl-s300 hr, q- and s-sepharoses and lentil lectin agarose. the fraction eluted from t ... | 1991 | 1850386 |
| isolation and characterization of a thermolabile beta-2 macroglycoprotein ('thermolabile substance' or 'hakata antigen') detected by precipitating (auto) antibody in sera of patients with systemic lupus erythematosus. | a novel thermolabile beta-2 macroglycoprotein ('thermolabile substance' (tls) or 'hakata antigen' (ha], which was detected by the precipitating (auto) antibodies of patients with systemic lupus erythematosus, was isolated and characterized. the purification procedure entailed the following steps: isoelectric precipitation in the range between ph 5.2-6.1, hydroxyapatite absorption chromatography, 35% saturated ammonium sulfate precipitation, sephadex g-200 gel filtration, pevikon block electropho ... | 1991 | 1859827 |
| characterization and partial purification of aim: a plasma protein that induces rat cerebral type 2 astroglia from bipotential glial progenitors. | studies on glial cultures have demonstrated that fetal bovine serum contains a factor that induces bipotential glial precursors known as oligodendrocyte-type 2 astrocyte (o-2a) progenitors to become type 2 astroglia rather than oligodendroglia. the goal of this research project was to characterize and purify this factor, which we refer to as the astroglia-inducing molecule (aim). using cultures enriched in o-2a progenitors, we determined that aim is present in human and bovine sera and that feta ... | 1991 | 1861150 |
| intraperitoneal toxicity of pea and lentil lectins in albino rats--effect on growth and osmotic fragility of erythrocytes. | lectins from peas and lentils when injected to rats apparently appeared to be non toxic but they caused growth depression. the organ weights were not affected except spleen enlargement. the lectins also caused increased osmotic fragility of erythrocytes without affecting other hematological parameters such as haemoglobin, packed cell volume, and rbc count. | 1991 | 1874544 |
| efficacy of siv/deltab670 glycoprotein-enriched and glycoprotein-depleted subunit vaccines in protecting against infection and disease in rhesus monkeys. | immunization with an inactivated whole-virus vaccine is highly effective in preventing lentivirus infection. the viral protein(s) essential to the induction of protective responses, however, have not been identified. to define the role of virion components in the induction of protective immunity, we evaluated the efficacy of glycoprotein-enriched and glycoprotein-depleted simian immunodeficiency virus (siv) subunit vaccines prepared by lentil-lectin affinity chromatography of gradient-purified v ... | 1991 | 1883540 |
| improvement of gelatin particle agglutination test for detection of anti-htlv-i antibody. | partial modifications of antigen components were made to improve the gelatin particle agglutination (pa) test for the detection of antibodies against human t cell leukemia virus type-i. envelope glycoproteins prepared by lentil lectin affinity chromatography were further added to the purified viral antigens to be coated on the gelatin particles. comparative studies with a conventional pa test kit (serodia atla) and indirect immunofluorescence assay showed that the specificity and sensitivity of ... | 1991 | 1904415 |
| further studies of lectin binding by villous and extravillous trophoblast in normal and pathological pregnancy. | examination of human pregnancy tissues with a panel of lectins provides the opportunity to probe different aspects of carbohydrate structure. nine biotinylated lectins [concanavalin a (con a), wheat germ agglutin (wga), lens culinaris a (lch-a), pisum sativum (psa), phaseolus vulgaris (pha-e and pha-l), ulex europaeus 1 (uea1), griffonia simplicifolia (gsi and gsii)] were used to investigate the lectin binding of human trophoblast in normal, tubal, and molar pregnancy. all lectins except uea1 bo ... | 1991 | 1917274 |
| rapid production of interferon-gamma in uninduced human leukocyte suspensions. | human leukocytes produced interferon-gamma (ifn-gamma) rapidly without induction during incubation at 37 degrees c. the intracellular level peaked at 3 h; the extracellular ifn-gamma reached its maximum at 7 h. leukocytes purified by different methods, and whether from single donors or pooled from numerous donors, yielded similar amounts of ifn. the best yields were approximately 3 iu/10(6) cells. the highest titers were obtained at cell concentrations ranging between 30 and 100 x 10(6)/ml, and ... | 1991 | 1919078 |
| glycoconjugates of the lateral prostate of the guinea pig: a lectin histochemical study. | the present study examined the glycoconjugates of the lateral prostate using a battery of lectins. the results indicated that the secretory epithelium was rich in mannose (man), n-acetylglucosamine (glcnac), galactose (gal), n-acetylgalactosamine (galnac), and complex oligosaccharides. con a (concanavalin a), lca (lens culinaris agglutinin), psa (pisum sativum agglutinin), wga (wheat germ agglutinin), pwm (pokeweed mitogen), rca-i (racinus communis isolectin i), and pha-p (phaseolus vulgaris agg ... | 1991 | 1923963 |