Publications

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qualitative effects of fresh and dried plum ingredients on vacuum-packaged, sliced hams.boneless ham muscles (semimembranosus+adductor) were injected (20% w/w) with a curing brine containing no plum ingredient (control), fresh plum juice concentrate (fp), dried plum juice concentrate (dp), or spray dried plum powder (pp) at 2.5% or 5%. hams were cooked, vacuum-packaged, stored at<4 degrees c and evaluated at 2-week intervals over 10 week. evaluations were performed on sliced product to determine cook loss, vacuum-package purge, allo-kramer shear force, 2-thiobarbituric acid-reactiv ...200920416628
cultivated grapevines represent a symptomless reservoir for the transmission of hop stunt viroid to hop crops: 15 years of evolutionary analysis.hop stunt was a mysterious disorder that first emerged in the 1940s in commercial hops in japan. to investigate the origin of this disorder, we infected hops with natural hop stunt viroid (hpsvd) isolates derived from four host species (hop, grapevine, plum and citrus), which except for hop represent possible sources of the ancestral viroid. these plants were maintained for 15 years, then analyzed the hpsvd variants present. here we show that the variant originally found in cultivated grapevines ...200920041179
application of feces extracts and synthetic analogues of the host marking pheromone of anastrepha ludens significantly reduces fruit infestation by a. obliqua in tropical plum and mango backyard orchards.we determined the efficacy of three potential oviposition deterrents in reducing fruit infestation by anastrepha obliqua in tropical plum and mango orchards. these were: (1) extracts of feces of mexican fruit fly, anastrepha ludens, known to contain the a. ludens host marking pheromone (hmp) and (2) two fully synthetic simplified analogues of the naturally occurring compound, which we have named desmethyl a. ludens hmp (dm-hmp) and anastrephamide. two applications of feces extracts 2 or 3 wk bef ...200920069857
siadenovirus infection in two psittacine bird species.consensus polymerase chain reaction was used to identify a novel adenovirus from two psittacine birds: a plum-headed parakeet (psittacula cyanocephala) with lethargy, weight loss, and marked leukocytosis; and an umbrella cockatoo (cacatua alba) with lethargy, weight loss, and feather abnormalities. phylogenetic and comparative sequence analysis suggested that this virus is a member of the genus siadenovirus, and is here termed psittacine adenovirus 2. this extends the characterized adenoviruses ...200919937528
[observation on therapeutic effect of electroacupuncture at jiaji (ex-b 2) combined with blood-letting and cupping on herpes zoster].to compare the therapeutic effect differences between electroacupuncture at jiaji (ex-b 2) combined with blood-letting plus cupping and western medicine therapy.200919994687
redesigning a large-scale clinical trial in response to negative external trial results: the camus study of phytotherapy for benign prostatic hyperplasia.benign prostatic hyperplasia (bph), a common condition among older men, confers its morbidity through potentially bothersome lower urinary tract symptoms. treatments for bph include drugs such as alpha-adrenergic receptor blockers and 5-alpha reductase inhibitors, minimally invasive therapies that use heat to damage or destroy prostate tissue, and surgery including transurethral resection of the prostate. complementary and alternative medicines are gaining popularity in the us. two phytotherapie ...200920007408
attraction of anoplophora glabripennis to male-produced pheromone and plant volatiles.the male-produced pheromone of anoplophora glabripennis (motschulsky) (coleoptera: cerambycidae), which is an equal blend of 4-(n-heptyloxy)butan-1-ol and 4-(n-heptyloxy)butanal, was used in laboratory bioassays and in the greenhouse to determine its potential for attracting a. glabripennis adults. in modified "walking wind tunnels," virgin females were most attracted to the alcohol component, and virgin males were repelled by the pheromone blend at the lowest and highest amounts offered. y-tube ...200920021771
[a meta analysis on randomized controlled trials of acupuncture treatment of chronic fatigue syndrome].to assess the effectiveness of acupuncture treatment of chronic fatigue syndrome (cfs).200920209981
monitoring of the entomological diversity in a pesticide free orchard: investigation of the gembloux agricultural university conservatory.in orchards, the fruit production is currently oriented to integrated pest management. in order to develop efficient pest control strategy by using entomophagous beneficial and lowering the application of pesticides, sufficient knowledge of entomological populations is needed. here, a systematic monitoring of insect diversity and abundance in a conservatory orchard of gembloux was performed. combining yellow traps and visual observations, 19 pest and 34 beneficial families were identified among ...200920222593
international multidimensional authenticity specification (imas) algorithm for detection of commercial pomegranate juice adulteration.the pomegranate fruit ( punica granatum ) has become an international high-value crop for the production of commercial pomegranate juice (pj). the perceived consumer value of pj is due in large part to its potential health benefits based on a significant body of medical research conducted with authentic pj. to establish criteria for authenticating pj, a new international multidimensional authenticity specifications (imas) algorithm was developed through consideration of existing databases and co ...200919249817
phenolic compounds in rosaceae fruits from ecuador.rp-hplc-dad was used to study the content of phenolic compounds in four ecuadorian fruits (strawberry, andean blackberry, plum, and capuli cherry). compounds were identified using spectral characteristics of representative standards and reference samples. further, lc-ms with ms/ms was used to confirm molecular assignments in previously unstudied capuli cherry. gallic acid was detected in andean blackberry, and galloyl esters were detected in strawberries. both these berries contained ellagic aci ...200919143537
development and testing of the gait assessment and intervention tool (g.a.i.t.): a measure of coordinated gait components.recent neuroscience methods have provided the basis upon which to develop effective gait training methods for recovery of the coordinated components of gait after neural injury. we determined that there was not an existing observational measure that was, at once, adequately comprehensive, scored in an objectively-based manner, and capable of assessing incremental improvements in the coordinated components of gait. therefore, the purpose of this work was to use content valid procedures in order t ...200919146879
viewpoint: dried plum, an emerging functional food that may effectively improve bone health.osteoporosis is a debilitating disorder that affects both female and male, albeit to a greater extent in women than men. as the demographic shift to a more aged population continues, a growing number of men and women will be afflicted with osteoporosis and a search for potential non-pharmacological alternative therapies for osteoporosis is of prime interest. aside from existing drug therapies, certain lifestyle and nutritional factors are known to reduce the risk of osteoporosis. among nutrition ...200919274852
differentiation of plum pox virus isolates by single-strand conformation polymorphism and low-stringency single specific primer pcr analysis of hc-pro genome region.single-strand conformation polymorphism (sscp) and low-stringency single specific primer (lssp)-pcr were assessed for suitability and reliability in genotyping of plum pox virus (ppv) isolates. examined ppv isolates included 16 ppv-d, 12 ppv-m, and 14 ppv-rec isolates collected in czech republic. the analysis was performed on the helper component protease (hc-pro) region of the ppv genome. sscp and lssp-pcr allowed the differentiation of ppv strain, but sscp was not able to distinguish isolates ...200919301952
partial molecular characterization of an unusual, recombinant plum pox virus isolate from bulgaria. 200919301955
characterization of a natural plum pox virus isolate bearing a truncated coat protein.plum pox virus (ppv) isolates were collected in hungary from plum varieties. pcr targeting the 3' genomic region resulted in a shorter pcr product in the case of the b1298 isolate bearing a 135-nucleotide deletion in frame in the n-terminal part of the coat protein (cp). the isolate was aphid-transmissible and the virion diameter was reduced compared to ppv-sk68. detectability of this isolate by western blot varied according to the antibody used. integration of the deleted cp gene into an infect ...200919082685
plum juice, but not dried plum powder, is effective in mitigating cognitive deficits in aged rats.normal aging in animals and humans is accompanied by a decline in cognitive performance that is thought to be due to the long-term effects of oxidative stress and inflammation on neurologic processes. previous findings have suggested that protection against age-related cognitive declines may be achieved by increasing the dietary intake of fruits and vegetables, especially those that are high in antioxidant activity, such as blueberries and strawberries. the objective of this study was to investi ...200919097856
is a plum a memory problem? longitudinal study of the reversal of concreteness effect in a patient with semantic dementia.the concreteness effect, which refers to the typically superior performance for concrete concepts compared to abstract ones, is a robust phenomenon that has been observed in normal and brain-damaged subjects in a number of cognitive domains. reversal of this effect was also reported in a few neuropsychological studies, mainly in patients with semantic dementia (sd). in this article, we report the first longitudinal single-case study of a patient with sd, sc, who performed better for abstract tha ...200918973766
effect of different formulations on tebuconazole residues in stone fruits.the correlation between pesticide residue levels and formulation of an active substance is often not considered, even if it is reasonable to expect some differences arising from behaviour during dilution and spraying, from adhesion to plant and from degradation. an experimental study to investigate the magnitude of tebuconazole residues as a function of different tebuconazole formulated products was carried out in italy. the fungicide was applied as wettable powder (wp) and water-dispersible gra ...200919165745
synteny of prunus and other model plant species.fragmentary conservation of synteny has been reported between map-anchored prunus sequences and arabidopsis. with the availability of genome sequence for fellow rosid i members populus and medicago, we analyzed the synteny between prunus and the three model genomes. eight prunus bac sequences and map-anchored prunus sequences were used in the comparison.200919208249
molecular characterization of seven genes encoding ethylene-responsive transcriptional factors during plum fruit development and ripening.seven erf cdnas were cloned from two japanese plum (prunus salicina l.) cultivars, 'early golden' (eg) and 'shiro' (sh). based on the sequence characterization, these ps-erfs could be classified into three of the four known erf families. their predicted amino acid sequences exhibited similarities to erfs from other plant species. functional nuclear localization signal analyses of two ps-erf proteins (ps-erf1a and -1b) were carried out using confocal microscopy. expression analyses of ps-erf mrna ...200919213809
new facility to advance study of foreign animal disease. kansas site recommended for national bio and agro-defense facility to replace plum island. 200919226691
clinical, anamnestic and serological features of peach allergy in portugal.peach is a common food allergen source throughout europe. the aim of this study was to characterize peach allergy in a portuguese patient population.200919033734
compromised virus-induced gene silencing in rdr6-deficient plants.rna silencing in plants serves as a potent antiviral defense mechanism through the action of small interfering rnas (sirnas), which direct rna degradation. sirnas can be derived directly from the viral genome or via the action of host-encoded rna-dependent rna polymerases (rdrs). plant genomes encode multiple rdrs, and it has been demonstrated that plants defective for rdr6 hyperaccumulate several classes of virus. in this study, we compared the effectiveness of virus-induced gene silencing (vig ...200919129420
[anaphylaxis due to peach with negative immunocap result to peach allergens, including rpru p 1, rpru p 3, and rpru p 4: a report of two cases].we report two cases of anaphylactic reactions to peach with negative result of immunocap to peach. case 1 is a 35-year-old man, who felt an itch in his oral cavity immediately after ingesting a whole fresh peach. he rapidly developed generalized urticaria, dyspnea, vomiting, and loss of consciousness. he recovered after treatment at a local hospital, thereafter he was referred to our hospital because immunocap conducted for screening allergens revealed a negative test result to peach and the cau ...200919329877
native electrophoresis and western blot analysis: method and applications.native electrophoresis and western blot analysis (neweb) has been developed for the study of plant virus characteristics, among others, virus particle-protein interactions, electrophorotype formation, and strain separation. the method is based on the property of electrophoretic mobility of virus particles (vp) and proteins and combines the analytical capacity of electrophoresis with the specificity of western blot. one of its advantages is that it deals with entire vp that can be studied in caus ...200919378066
cell-to-cell transport through plasmodesmata in tree callus cultures.one factor that contributes to a successful fruit tree grafting is the establishment of symplasmic contacts in the graft interface to facilitate the transfer of compounds between scion and stock. using novel experimental and theoretical approaches we investigated whether the localized incompatibility, experienced in some prunus grafts, could be related to insufficient plasmodesmal coupling at an early stage of development within one of the partners. dye-coupling analysis using fluorescent tracer ...200919398772
quantitative trait loci meta-analysis of plum pox virus resistance in apricot (prunus armeniaca l.): new insights on the organization and the identification of genomic resistance factors.plum pox virus (ppv) is responsible for sharka disease, one of the most detrimental stone fruit diseases affecting prunus trees worldwide. only a few apricot cultivars have been described as resistant, most originating from north american breeding programmes. several ppv resistance quantitative trait loci (qtls) have been mapped in various progenies, consistently highlighting the contribution to the resistance of the upper part of linkage group 1 (lg1). however, to date, no consensus has been re ...200919400838
specific detection and quantification of the phytopathogenic agent 'candidatus phytoplasma prunorum'.'candidatus phytoplasma prunorum' is a wall-less bacterium associated with european stone fruit yellows (esfy), a severe disease of prunus spp. (mainly apricot and japanese plum trees). it can be spread by one insect vector, cacopsylla pruni, and by the trade of infected material. the availability of pcr-based methods allowing a sensitive and specific detection of 'ca. p. prunorum' is crucial for this phytoplasma because, at present, it is uncultured and cannot be detected serologically. we deve ...200919401232
sensitive and rapid detection of peach latent mosaic viroid by the reverse transcription loop-mediated isothermal amplification.a reverse transcription loop-mediated isothermal amplification (rt-lamp) method for the detection of peach latent mosaic viroid (plmvd) was developed. four primer sets (old, old1, new, and fukuta's) were designed originally. based on initial experiments the set old1 was selected for further evaluation. simple and accelerated rt-lamp was preformed using degenerate and no degenerate forward-loop (f-loop) and backward-loop (b-loop) primers. degenerate primers were selected, and after determination ...200919409419
inhibitory effect of immature plum on pma-induced mmp-9 expression in human hepatocellular carcinoma.the goal of this study was to evaluate the anticancer effect of prunus salicina lindl. cv. soldam at three maturity stages (immature, midmature and mature). in search for anticancer effects of immature plum extract (ipe), we have found its antimigrative property in (phorbol 12-myristate 13-acetate) pma-induced hepg2 cells, and this effect is associated with inhibition of mmp-9 activity. ipe appeared to have a strong inhibitory effect on the pma-induced mmp-9 secretion through suppression of the ...200919418353
identification of the constituents of balsam of peru in tomatoes.studies show that balsam-restricted diets result in significant improvement of systemic contact dermatitis in patients with contact allergy to balsam of peru (bop). while tomatoes have been implicated as a frequent cause of bop-related dermatitis, the presence of bop in tomatoes has never been confirmed.200919426616
further characterization of a new recombinant group of plum pox virus isolates, ppv-t, found in orchards in the ankara province of turkey.sixteen plum pox virus (ppv) isolates collected in the ankara region of turkey were analyzed using available serological and molecular typing assays. surprisingly, despite the fact that all isolates except one, which was a mix infection, were typed as belonging to the ppv-m strain in four independent molecular assays, nine of them (60%) reacted with both ppv-m specific and ppv-d specific monoclonal antibodies. partial 5' and 3' genomic sequence analysis on four isolates demonstrated that irrespe ...200919428744
direct sample preparation methods for the detection of plum pox virus by real-time rt-pcr.direct systems to process plant materials allowed high-throughput testing of plum pox virus (ppv) by real-time reverse transcription (rt)-pcr without nucleic acids purification. crude plant extracts were diluted in buffer or spotted on membranes to be used as templates. alternatively, immobilized ppv targets were amplified from fresh sections of plant tissues printed or squashed onto the same supports, without extract preparation. spot real-time rt-pcr was validated as a ppv diagnostic method in ...200919440977
bone marrow-derived endothelial progenitor cells contribute to the angiogenic switch in tumor growth and metastatic progression.emerging evidence indicates that bone marrow (bm)-derived endothelial progenitor cells (epcs) contribute to angiogenesis-mediated growth of certain tumors in mice and human. epcs regulate the angiogenic switch via paracrine secretion of proangiogenic growth factors and by direct luminal incorporation into sprouting nascent vessels. while the contributions of epcs to neovessel formation in spontaneous and transplanted tumors and to the metastatic transition have been reported to be relatively low ...200919460418
'kwanzan stunting' syndrome: detection and molecular characterization of an italian isolate of little cherry virus 1.evident stunting was observed for the first time on prunus serrulata 'kwanzan' indicator trees in southern italy during the indexing of two sour cherry accessions from cultivars 'marasca di verona' and 'spanska'. bud break and shooting were delayed and the developing leaves remained small. during the third year many kwanzan plants died, regardless of the indexed cultivar. electrophoretic analysis showed the presence of dsrna pattern in extracts of stunted kwanzan with a similar size to that of v ...200919463722
electroantennogram technique for conotrachelus nenuphar (coleoptera: curculionidae).reliable electroantennogram (eag) responses were obtained from the plum curculio, conotrachelus nenuphar (herbst), by using a whole body mount with glass electrodes filled with kcl-saturated agcl solution that penetrated the antennal club (recording electrode) and the membrane attached to the cervical sclerite between the thorax and the head (indifferent electrode). known attractive odor sources including extracts of headspace collections of 'stanley' plum and 'empire' apple tissues and a synthe ...200919508798
identifying peach and plum polyphenols with chemopreventive potential against estrogen-independent breast cancer cells.our objective was to evaluate the cancer suppression activity of extracts from a commercial variety of yellow-fleshed peach 'rich lady' (rl) and a red-fleshed plum 'black splendor' (bs) and identify the phenolic fractions that may possess potential as chemopreventive and/or chemotherapeutic natural compounds. the peach rl extract effectively inhibited the proliferation of the estrogen-independent mda-mb-435 breast cancer cell line. the concentration to inhibit 50% of cell proliferation (ic(50)) ...200919530711
[pricking blood therapy combined with ultraviolet irradiation for treatment of acute herpes zoster].to evaluate clinical therapeutic effect and the safety of pricking blood therapy combined with ultraviolet irradiation for treatment of acute herpes zoster.200919565736
observations of atmospheric nitrogen and phosphorus deposition during the period of algal bloom formation in northern lake taihu, china.cyanobacterial blooms in lake taihu occurred at the end of april 2007 and had crucial impacts on the livelihood of millions of people living there. excessive nutrients may promote bloom formation. atmospheric nitrogen (n) and phosphorus (p) deposition appears to play an important role in algal bloom formation. bulk deposition and rain water samples were collected respectively from may 1 to november 30, 2007, the period of optimal algal growth, to measure the bulk atmospheric deposition rate, wet ...200919597869
quantitative estimation of plum pox virus targets acquired and transmitted by a single myzus persicae.the viral charge acquired and inoculated by single aphids in a non-circulative transmission is estimated using plum pox virus (ppv). a combination of electrical penetration graph and taqman real-time rt-pcr techniques was used to establish the average number of ppv rna targets inoculated by an aphid in a single probe (26,750), approximately half of the acquired ones. this number of ppv targets is responsible for a systemic infection of 20% on the inoculated receptor plants. no significant differ ...200919597934
[parasitoids (hymenoptera: braconidae) of fruit flies (diptera: tephritidae) in the state of são paulo, brazil: associated plants and parasitism].a total of 3,008 braconid parasitoids in 26 species of fruit fly host plants were collected in 33 localities of são paulo state. the majority of the braconids (96.2%) belonged to four opiinae species, doryctobracon areolatus (szépligeti), doryctobracon brasiliensis (szépligeti), opius bellus (gahan) and utetes anastrephae (viereck), and the remaining 3.8%, a single species of alysiinae, asobara anastrephae (muesebeck). doryctobracon areolatus was collected on anastrepha fraterculus (wied), a. ob ...200919618046
lower limb deformity due to failed trauma treatment corrected with the ilizarov technique: factors affecting the complication rate in 52 patients.failed treatment of fractures may be corrected by the ilizarov technique but complications are common. in 52 patients with compromised healing of femoral and tibial fractures, the results of secondary reconstruction with ilizarov treatment were investigated retrospectively in order to identify the factors that contribute to the risk of complications.200919626469
induction of apoptosis by immature plum in human hepatocellular carcinoma.in the present study, the effect of an extract of immature prunus salicina lindl. cv. soldam fruit on the viability and induction of apoptosis in human hepatocellular carcinoma hepg2 cells was investigated. the results showed that in comparison with other cancer cells, the growth inhibition exerted by immature plum extracts was greatest in hepg2. apoptosis in hepg2 cells mediated by immature plums was associated with "death receptor signaling." immature plum extracts significantly increased the ...200919627199
analysis of pesticide residues by fast gas chromatography in combination with negative chemical ionization mass spectrometry.a combination of fast gc with narrow-bore column and bench top quadrupole mass spectrometer (ms) detector in negative chemical ionization (nci) mode (with methane as reagent gas) is set up and utilized for the ultratrace analysis of 25 selected pesticides. the observed pesticides, belonging to the endocrine disrupting chemicals (edcs), were from different chemical classes. a comparative study with electron impact (ei) ionization was also carried out (both techniques in selected ion monitoring (s ...200919643423
quality of life in simultaneous pancreas-kidney transplant recipients.simultaneous pancreas-kidney transplantation (spk tx) allows dialysis and insulin therapy to be discontinued and improves the complications of diabetes mellitus type 1 (dm1). this study measure quality of life (qol) in spk transplant recipients and determine if there are differences in qol between these patients and those with dm1 in renal replacement therapy (rrt).200919674015
field efficacy of entomopathogenic fungi and nematodes targeting caged last-instar plum curculio (coleoptera: curculionidae) in michigan cherry and apple orchards.the plum curculio (conotrachelus nenuphar herbst) is a key pest of pome and stone fruit in eastern north america. we tested the efficacy of five pathogens over the course of three seasons in 10 michigan apple and cherry orchards, with introductions of larvae to caged pots containing pathogen-treated soil. the nematode steinernema riobrave was the most effective pathogen in the 2 yr it was tested, but only in soils with the highest sand content (81-88%) and when it was applied 1 h or 5 d after la ...200919689891
towards the routine use of brain imaging to aid the clinical diagnosis of disorders of consciousness.clinical audits have highlighted the many challenges and dilemmas faced by clinicians assessing persons with disorders of consciousness (vegetative state and minimally conscious state). the diagnostic decision-making process is highly subjective, dependent upon the skills of the examiner and invariably dictated by the patients' ability to move or speak. whilst a considerable amount has been learnt since jennett and plum coined the term 'vegetative state', the assessment process remains largely u ...200919710182
the determinant of potyvirus ability to overcome the rtm resistance of arabidopsis thaliana maps to the n-terminal region of the coat protein.in arabidopsis thaliana columbia (col-0) plants, the restriction of tobacco etch virus (tev) long-distance movement involves at least three dominant rtm (restricted tev movement) genes named rtm1, rtm2, and rtm3. previous work has established that, while the rtm-mediated resistance is also effective against other potyviruses, such as plum pox virus (ppv) and lettuce mosaic virus (lmv), some isolates of these viruses are able to overcome the rtm mechanism. in order to identify the viral determina ...200919737103
a new selective medium for the recovery and enumeration of monilinia fructicola, m. fructigena, and m. laxa from stone fruits.isolation of monilinia spp. from stone and pome fruit surfaces is difficult due to the presence of several fast-growing fungal species such as rhizopus, alternaria, and penicillium spp. therefore, a new selective medium (acidified potato dextrose agar [ph 3.6] amended with fosetyl-aluminum [fosetyl-al] at 500 microg/ml) (apda-f500) was developed for the recovery of monilinia propagules. the antifungal agents fosetyl-al, dichloran, ammonium molybdate, and 2-deoxy-d-glucose (2-dd-glucose) were tes ...200919740034
geographical location has greater impact on carotenoid content and bioaccessibility from tomatoes than variety.the suggested health benefits of consuming tomatoes and tomato-based products have been attributed, in part, to the carotenoids present in these foods. therefore, the objectives of the present study were to (i) analyse carotenoid content and bioaccessibility from different tomato (lycopersicon esculentum l.) types namely cherry, plum, round, and certain tomatoes-on-the-vine; and (ii) determine if geographical location (ireland vs spain) influenced the content and bioaccessibility of carotenoids ...200919757067
pesticide extraction from table grapes and plums using ionic liquid based dispersive liquid-liquid microextraction.room temperature ionic liquids (rtils) have been used as extraction solvents in dispersive liquid-liquid microextraction (dllme) for the determination of eight multi-class pesticides (i.e. thiophanate-methyl, carbofuran, carbaryl, tebuconazole, iprodione, oxyfluorfen, hexythiazox, and fenazaquin) in table grapes and plums. the developed method involves the combination of dllme and high-performance liquid chromatography with diode array detection. samples were first homogenized and extracted with ...200919779926
effectiveness of glues used for harmonic radar tag attachment and impact on survival and behavior of three insect pests.the ability of three cyanoacrylate glues to ensure a durable bond between the colorado potato beetle, leptinotarsa decemlineata (say), the plum curculio, conotrachelus nenuphar (herbst), or the corn rootworms (western corn rootworm, diabrotica virgifera virgifera leconte and northern corn rootworm, diabrotica longicornis smith and lawrence) and the harmonic radar tag without impact on behavior and survival was assessed as part of a study on the use of harmonic radar technology to track these ins ...200919791611
measurement of greenhouse gas emissions from agricultural sites using open-path optical remote sensing method.improved characterization of distributed emission sources of greenhouse gases such as methane from concentrated animal feeding operations require more accurate methods. one promising method is recently used by the usepa. it employs a vertical radial plume mapping (vrpm) algorithm using optical remote sensing techniques. we evaluated this method to estimate emission rates from simulated distributed methane sources. a scanning open-path tunable diode laser was used to collect path-integrated conce ...200919827493
a tale of two carboxypeptidases.proopiomelanocortin (pomc) neurons play a central role in energy homeostasis. despite the complexity of pomc posttranslational processing, regulation of pomc gene expression often takes center stage. complementary papers that zero in on distinct carboxypeptidases (plum et al., 2009; wallingford et al., 2009) now refocus the spotlight on regulated peptide cleavage.200919883612
curative activity of insecticides against plum curculio (coleoptera: curculionidae) in tart cherries.tart cherry, prunus cerasus l. variety montmorency, fruit were infested with plum curculio, conotrachelus nenuphar (herbst) (coleoptera: curculionidae), and treated with insecticides to target late instars, neonates, and eggs. the organophosphates azinphos-methyl and phosmet and the neonicotinoid thiamethoxam reduced larval emergence rates by >90% for all life stage targets; after >30 d, few surviving larvae were found inside fruit. acetamiprid and thiacloprid also had curative activity and yiel ...200919886451
analysis of phenolic compounds in six norwegian plum cultivars (prunus domestica l.).six european plum cultivars ( prunus domestica l.) grown in norway have been studied with respect to phenolic composition. neochlorogenic acid was found to be the most important phenolic acid in all cultivars. together with other phenolic acids, this compound varied significantly in amount among the cultivars. cyanidin 3-rutinoside was found to account for >60% of the total anthocyanin content. minor amounts of flavonols (rutin and quercetin 3-glucoside) were detected in all cultivars. total ant ...200919888727
non-carcinogenic carbon sorbents for respiratory protection.activated carbons impregnated with compounds of chromium, copper and silver (cr-cu-ag) are widely used for removing toxic substances from air, mainly in respiratory protection for industrial and military applications. toxicological and epidemiological studies have shown chromium (especially hexavalent cr) to have carcinogenic properties. hence, research has been launched to develop a new adsorption-catalytic complex without chromium, which is intended to replace the activated carbon-catalyst (cr ...200918752891
dried plums (prunes) reduce atherosclerosis lesion area in apolipoprotein e-deficient mice.dried plums are a fruit high in pectin with substantial antioxidant activity. previous studies in rats and man indicate that dried plums or plum fibre lower liver and plasma cholesterol, respectively. the apoe-deficient mouse, which develops atherosclerotic lesions rapidly when fed cholesterol, was used to determine the ability of dried plums to reduce atherosclerosis. diets containing 0.15% cholesterol and either 0 (b+c), 4.75% (lo dp) or 9.5% (hi dp) dried plum powder were fed for 5 months. an ...200918761779
dried plum polyphenols attenuate the detrimental effects of tnf-alpha on osteoblast function coincident with up-regulation of runx2, osterix and igf-i.previous studies have demonstrated that dried plums which contain high amounts of polyphenols can restore bone mass and structure, and significantly increase indices of bone formation. the purpose of this study was to determine how dried plum polyphenols influence osteoblast activity and mineralized nodule formation under normal and inflammatory conditions. mc3t3-e1 cells were plated and pretreated with dried plum polyphenols (0, 2.5, 5, 10 and 20 microg/ml) and 24 h later stimulated with tnf-al ...200918495459
molecular markers reveal strong geographic, but not host associated, genetic differentiation in aphidius transcaspicus, a parasitoid of the aphid genus hyalopterus.host plant associated genetic differentiation is a common phenomenon in phytophagous insects, but the degree to which such associations sequentially drive diversification at higher trophic levels is not as well understood. a recent study examining neutral molecular markers in hyalopterus aphids revealed that genetic structure in this genus is strongly determined by primary host plant use (prunus spp.). in this paper, we take a similar approach to determine whether this host plant specificity has ...200918662432
aperitif effects on gastric emptying: a crossover study using continuous real-time 13c breath test (breathid system).the aim of this study was to determine whether there is a correlation between aperitif and gastric emptying. ten healthy male volunteers participated in this randomized, two-way crossover study. under two conditions (after drinking an aperitif versus not), the (13)c breath test was performed for 4 h with a liquid meal (200 kcal/200 ml) containing 100 mg (13)c acetate. we used 50 ml of umeshu as the aperitif. this is a traditional japanese plum liqueur, and contains 7 ml alcohol (14%). in the ape ...200918688714
a temperature-controlled amplicon system derived from plum pox potyvirus.the control of replication can facilitate a viral amplicon to reach high expression levels by enabling the virus to escape host defence mechanisms and reducing the deleterious effects of viral infection. we have developed a novel system to regulate amplicon expression by controlling the temperature of plant growth. nicotiana benthamiana plants were transformed at two different temperatures with a cdna copy of the plum pox potyvirus genome harbouring the open reading frame 2 of porcine circovirus ...200918801011
expression analysis of a plum pathogenesis related 10 (pr10) protein during brown rot infection.plant pr10 is one of the pathogenesis related proteins, induced upon exposure to different stress conditions including fungal infection. pr10 proteins have been implicated in fungal disease resistance in some species; however its transcriptional regulation is not well understood. in the present work we cloned a pr10 gene from european plums (prunus domestica l.) and monitored the quantitative changes in its transcript levels as a result of fungal infection in two varieties. we also studied the p ...200918815787
immunostimulatory effects of oriental plum (prunus salicina lindl.).the fruit of the plum tree (prunus salicina lindl.) has been used as a traditional medicinal food in humans to enhance immunity against infectious agents and to treat cancers. however, limited information exists on the mechanisms responsible for its immune enhancing properties. in this study, the immunostimulatory effects of a methanol extract of plum fruit following methanol evaporation and dissolving in pbs were assessed by in vitro lymphocyte proliferation, tumor cell cytotoxicity, and nitric ...200918262269
analyzing a liquid-solid phase countercurrent two- and three-stage adsorption process with the freundlich equation.adsorbent consumption advantages of a countercurrent two- and three-stage process are described. the freundlich equation and equilibrium-stage were used to deduce these adsorption processes; it was proved that large adsorbent savings were obtained from operations of these systems in most cases. microporous activated carbon was prepared from plum kernels with koh chemical activation. isotherm equilibrium adsorptions of three dyes (bb1, mb, and ab74) and three phenols (phenol, 4-cp, and 2,4-dcp) w ...200918556116
to address accuracy and precision using methods from analytical chemistry and computational physics.in this work the pesticides were determined by liquid chromatography-mass spectrometry (lc-ms). in present study the occurrence of imidacloprid in 343 samples of oranges, tangerines, date plum, and watermelons from valencian community (spain) has been investigated. the nine additional pesticides were chosen as they have been recommended for orchard treatment together with imidacloprid. the mulliken population analysis has been applied to present the charge distribution in imidacloprid. partition ...200918369729
changes in blood pesticide levels in booted eagle (hieraaetus pennatus) associated with agricultural land practices.we estimated the degree of exposure to lindane and endosulfan in the blood of booted eagle nestlings (hieraaetus pennatus) (1999-2003), in order to assess the usefulness of these samples as a unit for monitoring changes in exposure as a result of shifts in agricultural practices and the implementation of legal measures. the highest blood lindane concentrations were obtained 1 year prior to its prohibition by the european union. subsequent to that year, the drop in blood concentrations was dramat ...200918417218
structural determination of prunusins a and b, new c-alkylated flavonoids from prunus domestica, by 1d and 2d nmr spectroscopy.prunusins a (1) and b (2), the new c-alkylated flavonoids, have been isolated from the seed kernels of prunus domestica. their structures were assigned from (1)h and (13)c nuclear magnetic resonating spectra, dept and by correlation spectroscopy, hmqc and hmbc experiments. 3, 5, 7, 4'-tetrahydroxyflavone (3) and 3, 5, 7-trihydroxy-8, 4'-dimethoxyflavone (4) have also been reported from this species. both compounds (1) and (2) showed significant antifungal activity against pathogenic fungus trich ...201019918802
influence of indigenous yeasts on the fermentation and volatile profile of plum brandies.the aim of this study was to determine the influence of different yeasts isolated from fresh blue plum fruits (aureobasidium sp.) and spontaneously fermenting plum musts (kloeckera apiculata and saccharomyces cerevisiae), as well as commercial wine and distillery strains, on the fermentation and chemical composition of plum brandies. gas chromatography methods were used to detect major volatile components. the most rapid fermentation occurred in musts inoculated with s. cerevisiae. however, the ...201020227608
domain-growth kinetic origin of nonhorizontal phase coexistence plateaux in langmuir monolayers: compression rigidity of a raft-like lipid distribution.the present work addresses the question of a nonhorizontal coexistence plateau found in the liquid-expanded (le) to liquid-condensed (lc) transition of langmuir monolayers of lipid amphiphiles, which is apparently incongruent with the first-order character of this main le/lc phase transition. this pathology is understood in a mechanical context as a resistance of the monolayer against compression giving rise to a nonzero rigidity in the coexistence region. surface rheology has allowed for a quan ...201020235509
harmonic radar: assessing the impact of tag weight on walking activity of colorado potato beetle, plum curculio, and western corn rootworm.the impact of electronic dipole tags on the walking activity of three insects was determined using video tracking software. results varied within and between the three species studied. the heaviest tag mounted on the pronotum of the colorado potato beetle, leptinotarsa decemlineata (say), reduced its mean horizontal speed slightly but significantly. the mean horizontal speed of plum curculio, conotrachelus nenuphar (herbst), was significantly reduced by a light tag but not by heavier tags. the m ...201020214369
putative recombination events and evolutionary history of five economically important viruses of fruit trees based on coat protein-encoding gene sequence analysis.to enhance the knowledge of recombination as an evolutionary process, 267 accessions retrieved from genbank were investigated, all belonging to five economically important viruses infecting fruit crops (plum pox, apple chlorotic leaf spot, apple mosaic, prune dwarf, and prunus necrotic ringspot viruses). putative recombinational events were detected in the coat protein (cp)-encoding gene using recco and rdp version 3.31beta algorithms. based on recco results, all five viruses were shown to conta ...201020035376
compositional changes in cell wall polysaccharides from japanese plum ( prunus salicina lindl.) during growth and on-tree ripening.climacteric japanese plums were harvested at six developmental stages with no intermediate storage period, and cell wall compositional changes were analyzed. arabinose proved to be the principal neutral monosaccharide constituent in cell walls during growth and the most dynamic neutral sugar in pectic fractions. arabinose loss from tightly bound pectins was found to be a relatively early feature in the sequence of cell wall biochemical modifications, thus suggesting a softening-related role duri ...201020099830
total folate: diversity within fruit varieties commonly consumed in india.folate concentrations in selected fruits were measured using the trienzyme extraction and microbiological assay with lactobacillus casei (subsp. rhamnosus) as an assay organism. fruits were purchased from different retail outlets at coimbatore, tamilnadu, india and were analyzed for total folate content. the folate content in all fruits varied considerably on a fresh weight basis from 10 to 328 microg/100 g, with tropical fruits ranging between 10 and 211 microg/100 g, temperate fruits from 11 t ...201020128636
combining fructooligosaccharide and dried plum has the greatest effect on restoring bone mineral density among select functional foods and bioactive compounds.functional foods and/or their bioactive compounds playing a role in improving skeletal health have received considerable attention. the objective of the present study was to determine the extent to which certain functional foods as (1) whole, e.g., dried plum (dp), figs, dates, raisin, and blueberry, (2) fractionated, e.g., dp puree, dp juice, and dp pulp/skin, or (3) isolated, e.g., dp polyphenols, fructooligosaccharides (fos), and beta-hydroxy-beta-methylbutyrate, forms reverse bone loss in an ...201020132045
purunusides a-c, alpha-glucosidase inhibitory homoisoflavone glucosides from prunus domestica.purunusides a-c (1-3), new homoisoflavone glucosides together with the known compounds beta-sitosterol (4) and 6,7-methylenedioxy-8-methoxycoumarin (5) have been isolated from n-butanol and ethyl acetate soluble fractions of prunus domestica. their structures were assigned on the basis of spectral studies. the compounds 1-3 showed potent inhibitory activity against the enzyme alpha-glucosidase.201020162398
prebiotic effect of fruit and vegetable shots containing jerusalem artichoke inulin: a human intervention study.the present study aimed to determine the prebiotic effect of fruit and vegetable shots containing inulin derived from jerusalem artichoke (ja). a three-arm parallel, placebo-controlled, double-blind study was carried out with sixty-six healthy human volunteers (thirty-three men and thirty-three women, age range: 18-50 years). subjects were randomised into three groups (n 22) assigned to consume either the test shots, pear-carrot-sea buckthorn (pcs) or plum-pear-beetroot (ppb), containing ja inul ...201020187995
reduction of campylobacter jejuni and campylobacter coli in poultry skin by fruit extracts.campylobacter spp. are a major cause of foodborne bacterial gastroenteritis in humans, and current methods to control campylobacter contamination in foods are not completely successful. plants are a promising source of antimicrobial agents, particularly given the growing interest in "all natural" foods. in this study, the antimicrobial activity of extracts from 28 edible plants against campylobacter jejuni and campylobacter coli was evaluated in vitro and in a poultry skin model. nine of 28 extr ...201020202332
regulation of two germin-like protein genes during plum fruit development.germin-like proteins (glps) have several proposed roles in plant development and defence. two novel genes (ps-glp1 and 2) encoding germin-like protein were isolated from plum (prunus salicina). their regulation was studied throughout fruit development and during ripening of early and late cultivars. these two genes exhibited similar expression patterns throughout the various stages of fruit development excluding two important stages, pit hardening (s2) and fruit ripening (s4). during fruit devel ...201020202999
type of snack influences satiety responses in adult women.the effect of different snack foods on satiety and plasma glucose and hormone responses was assessed. nineteen fasted adult women (mean age: 39.2 + or - 0.7 years, mean bmi: 26.1 + or - 0.8 kg/m(2)) consumed test foods including dried plums, low-fat cookies, white bread and water only on separate days. the test foods (with the exception of water) provided 238 kcal and were similar in total carbohydrate, fat and protein content but differed in fiber and sugar content. subjects rated their feeling ...201020206217
a single amino acid mutation alters the capsid protein electrophoretic double-band phenotype of the plum pox virus strain ppv-rec.plum pox virus (ppv) isolates differ by their capsid protein (cp) mobility in sds-page. these electrophoretic phenotypes are likely to result from post-translational modifications of the cp. we demonstrated that the cp mobility was solely determined by the cp n-terminal region. sequence comparison pinpointed a possible role of mutations at position 66 in determining the cp phenotype of ppv-rec isolates. site-directed mutagenesis of a chimeric clone demonstrated that gly(66) in the cp resulted in ...201020422234
identification and molecular characterization of a new potyvirus from panax notoginseng.a potyvirus causing distortion and mosaic symptoms in the herbal plant sanqi (panax notoginseng) was isolated from yunnan province, china, and the complete nucleotide sequence of one isolate and the partial sequences of two other isolates were determined. the viral rna genome comprised 9,750 nt excluding the 3'-terminal poly(a) tail, with the capacity to encode a single polyprotein of 3,089 amino acids. phylogenetic analysis with other completely sequenced potyviruses revealed that the virus in ...201020446003
the need for culture collections to support plant pathogen diagnostic networks.plant-pathogenic microorganisms, by virtue of their size, similarity in disease symptoms and closely related morphologies, are notoriously difficult to diagnose and detect. diagnosis gives proof as to the causal agent of disease and is important for developing appropriate control measures. detection shows the presence of a microorganism and is of importance for safeguarding national and international trade. live reference collections are required to characterize the taxonomy and function of micr ...201020457251
adsorption of copper onto agriculture waste materials.mining activities generate large amounts of wastewater, which contain heavy metals of elevated concentration according to legislative threshold values. therefore, treatment is necessary, and there has been a recent focus on more environmentally friendly methods. one of these is biosorption, where heavy metals are adsorbed from the wastewater via materials of biological origin. in this work copper sorption capacity, kinetics and isotherms of different low-cost residual agricultural materials was ...201020462688
correlation between the composition of multivalent antibody conjugates with colloidal gold nanoparticles and their affinity.interactions between multivalent preparations of antibodies (conjugated with colloidal gold nanoparticles (gnp) as a carrier system) and a multivalent ligand were investigated. the aim of the present study was to reveal the relationship between the affinity of the conjugate and its composition (i.e., the valency). surface plasmon resonance was applied to study the affinity and the kinetics of the interaction of multivalent conjugates and multivalent virus (on the example of the plum pox virus (p ...201020347832
sensory, chemical, and electronic tongue assessment of micro-oxygenated wines and oak chip maceration: assessing the commonality of analytical techniques.micro-oxygenation (mox) was conducted in the presence and absence of oak chips at rates to mimic oxygen ingress during barrel maturation of red wine. following mox, wines were analyzed for chemical attributes pertaining to phenolic composition and assessed by a trained sensory panel. an electronic tongue (et) was also used to assess the wines. variations in chemical attributes were found to be mostly influenced by vintage, followed by oak chip maceration accounting for 48% and 16% of variation w ...201020356080
development and validation of an algorithm to establish a total antioxidant capacity database of the us diet.estimation of total antioxidant intake is the first step to investigate the protective effects of antioxidants on oxidative stress-mediated disease. the present study was designed to develop an algorithm to estimate total antioxidant capacity (tac) of the us diet. tac of individual antioxidants and 50 popular antioxidant-rich food items in the us diet were determined by 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (abts) assay and the 1,1-diphenyl-2-picrylhydrazyl (dpph) assay. theoreti ...201020377495
analysis of wolbachia strains associated with conotrachelus nenuphar (coleoptera: curculionidae) in the eastern united states.we studied the distribution patterns of wolbachia infection associated with plum curculio strains in eight states of the eastern united states. the presence of the wolbachia-specific gene wsp identified infections of this endosymbiont in 97.8% of the 93 samples tested. three distinct wolbachia strains were identified. the strains wcne1 (593 bp) and wcne2 (593 bp) were 97% identical, and their sequences were both 84% identical with wcne3 (590 bp). blastn searches through genbank showed strong sim ...201020388268
rna-dependent rna polymerase 1 from nicotiana tabacum suppresses rna silencing and enhances viral infection in nicotiana benthamiana.endogenous eukaryotic rna-dependent rna polymerases (rdrs) produce double-stranded rna intermediates in diverse processes of small rna synthesis in rna silencing pathways. rdr6 is required in plants for posttranscriptional gene silencing induced by sense transgenes (s-ptgs) and has an important role in amplification of antiviral silencing. whereas rdr1 is also involved in antiviral defense in plants, this does not necessarily proceed through triggering silencing. in this study, we show that nico ...201020400679
active carbons prepared by chemical activation of plum stones and their application in removal of no2.a technology of obtaining active carbon from plum stones by chemical activation with koh is described. the effect of carbonisation temperature as well as activation procedure on the textural parameters, acid-base character of the surface and sorption properties of active carbons has been checked. the sorption properties of the activated carbons obtained were characterised by determination of nitrogen dioxide adsorption in dry and wet conditions. the final products were microporous activated carb ...201020576355
polarographic assay based on hydrogen peroxide scavenging in determination of antioxidant activity of strong alcohol beverages.total antioxidant (ao) activity of strong alcohol beverages such as wine and plum brandies, whiskeys, herbal and sweet fruit liqueurs have been assessed using a polarographic assay based on hydrogen peroxide scavenging (hps). rank of order of total ao activity, expressed as percentage of decrease of anodic oxidation current of hydrogen peroxide, was found analogous with total phenolic content estimated by folin-ciocalteau (fc) assay and radical scavenging capacity against the stable free radical ...201020604507
fruit and vegetable consumption among older adults by tooth loss and socio-economic status.the aim of this study was to examine consumption of fruit and vegetables in relation to tooth loss and income.201020604755
constraints to virus infection in nicotiana benthamiana plants transformed with a potyvirus amplicon.plant genomes have been transformed with full-length cdna copies of viral genomes, giving rise to what has been called 'amplicon' systems, trying to combine the genetic stability of transgenic plants with the elevated replication rate of plant viruses. however, amplicons' performance has been very variable regardless of the virus on which they are based. this has boosted further interest in understanding the underlying mechanisms that cause this behavior differences, and in developing strategies ...201020604920
analysis of expressed sequence tags from prunus mume flower and fruit and development of simple sequence repeat markers.expressed sequence tag (est) has been a cost-effective tool in molecular biology and represents an abundant valuable resource for genome annotation, gene expression, and comparative genomics in plants.201020626882
o-antigen diversity and lateral transfer of the wbe region among vibrio splendidus isolates.the o-antigen is a highly diverse structure expressed on the outer surface of gram-negative bacteria. the products responsible for o-antigen synthesis are encoded in the wbe region, which exhibits extensive genetic diversity. while heterogeneous o-antigens are observed within vibrio species, characterization of these structures has been devoted almost exclusively to pathogens. here, we investigate o-antigen diversity among coastal marine vibrio splendidus-like isolates. the wbe region was first ...201020629700
plum-branch-like carbon nanofibers decorated with sno2 nanocrystals.novel plum-branch-like carbon nanofibers (cnfs) decorated with sno2 nanocrystals have been synthesized by electrospinning and subsequent thermal treatment in an ar/h2o atmosphere. the morphologies of the as-synthesized sno2/cnf composites and the contents of carbon and sno2 can be controlled by adjusting the heat treatment temperature. it is proposed that the growth of sno2/cnf composites follows the outward diffusion of tin composites from the as-spun tin composite/polyacrylonitrile (pan) nanof ...201020648300
benzothiadiazole and l-2-oxothiazolidine-4-carboxylic acid reduce the severity of sharka symptoms in pea leaves: effect on antioxidative metabolism at the subcellular level.the effect of treatment with benzothiadiazole (bth) or l-2-oxothiazolidine-4-carboxylic acid (otc), and their interaction with plum pox virus (ppv) infection, on antioxidative metabolism of pea plants was studied at the subcellular level. ppv infection produced a 20% reduction in plant growth. pre-treatment of pea plants with otc or bth afforded partial protection against ppv infection, measured as the percentage of leaves showing symptoms, but neither bth nor otc significantly reduced the virus ...201020653891
coniochaeta (lecythophora), collophora gen. nov. and phaeomoniella species associated with wood necroses of prunus trees.species of the genus coniochaeta (anamorph: lecythophora) are known as pathogens of woody hosts, but can also cause opportunistic human infections. several fungi with conidial stages resembling lecythophora were isolated from necrotic wood samples of prunus trees in south africa. in order to reveal their phylogenetic relationships, these fungi were studied on a morphological and molecular (5.8s nrdna, its-1, its-2, gapdh, ef-1alpha, 28s nrdna, 18s nrdna) basis. some of the isolates were identifi ...201020664761
[relationship between ige antibodies to recombinant allergens rbet v 1 and rbet v 2 and food causing oral allergy syndrome in cases of birch-pollen allergy].persons allergic to birch pollen often report oral and pharyngeal hypersensitivity to fruit and vegetables, such as apples and peaches due to immunological cross-reactivity, or oral allergy syndrome (oas) sometimes accompanied by systemic reaction. such cross-reactive antigen reactions involve bet v 1, the main birch-pollen allergen, and bet v 2, birch-pollen profilin. we evaluated the food/antigen relationship.201020845708
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