Publications

anatomical and physiological evidence for a cerebellar nucleo-cortical projection in the cat.	combined neuroanatomical and electrophysiological experiments were performed to test the hypothesis that axon collaterals of neurons in the cerebellar nuclei project to the cerebellar cortex in cats. the anatomical studies demonstrated that (a) following the injection of tritiated leucine into the deep cerebellar nuclei, labeled fibers could be traced into the granular layer of the cerebellar cortex, and (b) following the injection of horseradish peroxidase into the cerebellar cortex, retrograde ...	1976	11370232
morphological correlates of visual field transformation in the corpus callosum.	horseradish peroxidase (hrp) injections at the boundary of areas 17 and 18 in cat visual cortex resulted in retrograde labelling of neurones located in contralateral areas 17 and 18. the hrp-positive neurones were spread over a region located around the representation of the vertical meridian; they were most numerous in the locus for area centralis. layer iii pyramidal cells and stellate cells in upper layer iv were identified as the principal neurones of origin of the visual callosal pathway.	1976	19604766
substantia nigra projections to the reticular formation, superior colliculus and central gray in the rat, cat and monkey.	after horseradish peroxidase injections in different parts of the lower brain stem retrogradely labeled neurons were observed in the substantia nigra, mainly ipsilateral pars reticulata. these findings demonstrate that the substantia nigra projects not only to the striatum and thalamus but also to brain stem areas which give rise to descending spinal projections. these nigral projections to brain stem structures may play an important role in a variety of behaviors and may be involved in clinical ...	1976	19604767
projections to gyrus sigmoideus from the substantia nigra in the cat, as revealed by the horseradish peroxidase retrograde transport technique.	after injections of horseradish peroxidase in the gyrus sigmoideus of the cat frontal cortex, labelled neurones were found in the substantia nigra and the ventral tegmental area of tsai. neurones distributed bilaterally clearly predominated in the side of the injection in the rostral sections, but differences in number were less at caudal levels. the present findings confirm the existence of a direct mesencephalocortical projection from this ventral tegmental region, and provide a morphological ...	1976	19604816
combined light and electron microscopic tracing of neurons, including axons and synaptic terminals, after intracellular injection of horseradish peroxidase.	by using intracellular deposition of horseradish peroxidase (hrp), a method has been designed for combined light and electron microscopic tracing of single fibers and synaptic terminals, belonging to physiologically characterized neurons. this technique has been successfully tested on the axon collateral systems of cat alpha-motoneurons, but should also be applicable to other types of neurons.	1976	19604850
the absence of horseradish peroxidase uptake by cerebellar afferents to the red nucleus of the cat.	horseradish peroxidase (hrp) administered to the red nucleus (rn) failed to be transported retrogradely in the axons of the cerebellorubral projection system. this negative result was independent of the anaesthesia, fixation method, injection time or survival period. the absence of hrp uptake and transport in our work reflects some uncertainty in the general usefulness of the hrp technique.	1976	19604851
simultaneous visualization of chromatolytic neurons and neurons retrogradely labeled with horseradish peroxidase.	a method is described for visualization of neurons retrogradely labeled with horseradish peroxidase (hrp) in celloidin-embedded brain slabs. the preservation of cellular detail obtained with this technique facilitates (1) accurate delimination of nuclei or laminae in which hrp-positive neurons are found and (2) simultaneous identification of cells of origin of two central fiber systems, by combining the retrograde transport of hrp and the retrotrade degeneration technique. the latter approach ha ...	1976	19604881
spinocervical tract cells in cat and dog, labeled by the retrograde transport of horseradish peroxidase.	the location of cells of origin of the spinocervical tract has been determined in cat and dog by injecting horseradish peroxidase into the lateral cervical nucleus (lcn). retrogradely labeled cells were found predominantly in the ipsilateral nucleus proprius of the dorsal horn along the entire spinal cord. cells of clarke's column and contralateral ventral horn cells were not labeled, in agreement with previous electrophysiological indications that the spinocerebellar tracts do not maintain coll ...	1976	19604882
cells of origin of hypothalamo-spinal projections in the rat.	the retrograde transport of horseradish peroxidase (hrp) was used to determine the distribution of hypothalamic neurons which send axonal projections to the spinal cord. labeled cells were observed in the paraventricular nucleus (pv) and in the lateral hypothalamic area following hrp injections into the lower thoracic and upper lumbar spinal cords of rats. in pv, the hrp-labeled cells were located in the parvocellular portion of the nucleus. hypothalamo-spinal projections are predominantly uncro ...	1976	19604883
retrograde labeling of central nervous pathways with tritiated or evans blue-labeled bovine serum albumin.	in an attempt to develop a technique for the retrograde tracing of neuronal pathways which does not depend on enzyme activity of horseradish peroxidase (hrp), we have examined the retrograde transport of bovine serum albumin (bsa) by neurons of the central nervous system. following injections of either tritiated or evans blue-labeled bsa into the hippocampal formation of the rat, labeled neurons were found in the ipsilateral entorhinal area in a pattern which was highly reminiscent of that obtai ...	1976	19604885
golgi-like staining of neocortical neurons using retrogradely transported horseradish peroxidase.	neocortical pyramidal neurons stained solidly with horseradish peroxidase (hrp) two days after striatal, thalamic or cortical hrp injections are described in the rabbit, rat and marmoset monkey. the dendritic detail seen in these solid hrp-labeled neurons approaches that produced by the golgi method.	1976	19604892
cells of origin of cortical projections to dorsal column nuclei, spinal cord and bulbar medial reticular formation in the rhesus monkey.	cells of origin of cortical fibers to the dorsal column nuclei, spinal cord and bulbar medial reticular formation were identified in 8 rhesus monkeys by means of the horseradish peroxidase (hrp) retrograde transport technique. after dorsal column nuclei injections small pyramidal neurons were labeled in the contralateral hemisphere in layer v of areas 3, 1, 2 and 5 and in layer v of the posterior part of area 4. in contrast, after spinal cord injections many area 4 giant betz cells in the contra ...	1976	19604894
surface membrane carbohydrate alterations of a flagellated protozoan mediated by bacterial endosymbiotes.	crithidia oncopelti, a parasitic trypanosomatid protozoan of insects, normally contains intracellular symbiotic bacteria. as shown earlier, the protozoa can be rid of their endosymbiotes by chloramphenicol, producing a symbiote-free cell line. here surface-membrane carbohydrate ligands of the symbiote-containing and symbiote-free strains were compared by lectin-mediated agglutination, lectin-ultrastructure localization. [3h] lectin-binding, and fluorescent lectin staining. symbiote-free organism ...	1976	1062797
covalent structure of the glycoprotein horseradish peroxidase (ec 1.11.1.7).		1976	1001465
[detection of antinuclear factor with horseradish peroxidase-conjugated antibody].		1976	798712
centrifugal fibers to the eye in a nonavian vertebrate: source revealed by horseradish peroxidase studies.	a source of efferent fibers to the eye of snakes of the genus thamnophis has been identified by the use of the retrograde transport of horseradish peroxidase. cell bodies of the contralateral nucleus of the ventral supraoptic decussation accumulate horseradish peroxidase after intraocular but not intraorbital injections. intraocular injections also result in anterograde transport of horseradish peroxidase to retinofugal axon terminals. intraorbital injections result in accumulation of horseradis ...	1976	63147
rhombo-mesencephalic connections in the fast conducting electrosensory system of the mormyrid fish, gnathonemus petersii. an hrp study.	using retrograde horseradish peroxidase (hrp) axonal transport direct rhombo-mesencephalic connections were revealed in gnathonemus petersii. by hrp injection in the neuropil of the extrolateral mesencephalic ganglion the neurons of the lateral line lobe nucleus were labeled. the pathway is mainly composed of crossed fibers.	1976	19604893
receptive fields, geometry and conduction block of sensory neurones in the central nervous system of the leech.	1. in segmental ganglia of the leech, the cutaneous mechanosensory neurones responding to to touch innervated the skin of their own segment and of part of the anterior and posterior adjacent segments. each touch receptive field could be divided into three non-overlapping areas: a central part innervated by the branches of the cell which ran in the nerve roots of the ganglion containing the cell body, and anterior and posterior parts innervated by its branches which ran in the nerve roots of the ...	1976	1018277
physiological properties and receptive fields of mechanosensory neurones in the head ganglion of the leech: comparison with homologous cells in segmental ganglia.	a study of the head ganglion of the leech was made to compare the properties of specific sensory cells in this ganglion with those of homologous neurones in the segmental ganglia. 1. in the head ganglion, cells were identified that had electrical properties, sensory modalities and adaptation properties similar to those of touch (t), pressure (p) and nociceptive (n) cells in the segmental ganglia. the cell bodies of these neurones were situated in characteristics positions that could be correlate ...	1976	1018276
a horseradish peroxidase method for the identification of the efferents of acetyl cholinesterase-containing neurons.		1976	826585
[problems posed by the interpretation of peroxidase labelling of various neurons in cyprinidae].	the origin of a centrifugal visual pathway in cyprinids could not be demonstrated with the technique involving the labelling of cell bodies by retrograde transport of horseradish peroxidase. the hypothalamic labelling following intraocular injection of hrp is localized in neurosecretory structures which take up the enzyme that has passed into the circulatory system. identical results were obtained following direct intracardiac injection. thus extreme caution must be taken in attempting to interp ...	1976	827368
the shapes of sensory and motor neurones and the distribution of their synapses in ganglia of the leech: a study using intracellular injection of horseradish peroxidase.		1976	12513
effects of apomorphine and haloperidol on somatopetal transport of horseradish peroxidase in the nigro-striatal pathway.	labeling of cell bodies in pars compacta of substantia nigra (snc) following injections of horseradish peroxidase (hrp) in the neostriatum was not affected by repeated systemic injections of apomorphine or haloperidol during the survival time. these drugs decelerate and accelerate, respectively, the firing of snc neurons. provided the used standard method was sensitive enough, it could be concluded that at least in these neurons the somatopetal transport and accumulation of the tracer do not dep ...	1976	19604884
compound x. an intermediate in enzymatic halogenation.	previous studies have shown that chlorite serves as a halogenation substrate for horseradish peroxidase. in its substrate role, chlorite serves both as a halogen donor and as a source of oxidizing equivalents in the chlorination reaction. we now show that a new spectral intermediate, which we have termed compound x, can be detected as the initial product of the reaction of chlorite with horseradish peroxidase. the reaction of chlorite with horseradish peroxidase to form compound x is a relativel ...	1976	10296
permeability of the amphibian pars intermedia to peroxidase injected intravascularly.	to study the penetration of protein from the blood into and through the pars intermedia of anurans, the electron-dense tracer, horseradish peroxidase (hrp), was injected into the vascular system of the frog, rana pipiens. pinocytotic vesicles were seen along plasma membranes of the stellate cells abutting the pericapillary spaces between the pars intermedia and pars nervosa. the hrp was seen within both the pericapillary spaces and these pinocytotic vesicles. in addition, the hrp was very promin ...	1976	1086719
stoichiometry of the reaction between horseradish peroxidase and p-cresol.	over a wide range of ph horseradish peroxidase compound i can be reduced quantitatively via compound ii to the native enzyme by only 1 molar equivalent of p-cresol. since 2 molar equivalents of electrons are required for the single turnover of the enzymatic cycle, p-cresol behaves as a 2-electron reductant. with p-cresol and compound i in a 1:1 ratio compound ii and p-methylphenoxy radicals are obtained in the transient state. compound ii is then reduced to the native enzyme. a possible explanat ...	1976	9412
oxidation of p-cresol by horseradish peroxidase compound i.	rate constants for the reaction between horseradish peroxidase compound i and p-cresol have been determined at several values of ph between 2.98 and 10.81. these rate constants were used to construct a log (rate) versus ph profile from which it is readily seen that the most reactive form of the enzyme is its most basic form within this ph range so that base catalysis is occurring. at the maximum rate a second order rate constant of (5.1 +/- 0.3) x 10(-7) m-1 s-1 at 25 degrees is obtained. the ac ...	1976	9411
[stabilization of polyacrylamide gel immobilized horseradish peroxidase by its covalent coupling to albumin].	pretreatment of peroxidase by its covalent coupling to inert proteins and albumin by means of glutaraldehyde considerably increases the thermostability and specific activity of polyacrylamide gel (paag) immobilized peroxidase. the effects of paag composition on the catalytic properties of the immobilized oligomers: peroxidase-inert proteins-albumin, are studied. the oligomers immobilized in 40% paag (10% n,n'-methylenebisacrylamide) possess the maximal specific activity (4.5 nmol/g). the effects ...	1976	1024581
preparation and properties of horseradish peroxidase bound covalently to polystyrene beads. application in the semi-automatic determination of hydrogen peroxide with homovanillic acid as substrate.		1976	973687
ultrastructural localization of immunoglobulins in skin of patients with dermatitis herpetiformis.	a multistep immunocytochemical method utilizing horseradish peroxidase as an immunologically bound marker was used to detect and localize iga deposits in skin of patients with dermatitis herpetiformis at the ultrastructural level. iga was found in the upper papillary dermis forming irregular aggregates in seemingly haphazard distribution. these aggregates were associated with microfibrillar bundles and with the microfibrillar component of the elastic tissue. iga was also detected on anchoring fi ...	1976	972258
studies on intra-arterial cushions. ii. distribution of horseradish peroxidase in cushions at the origins of intercostal arteries in mice.	the distribution of horseradish peroxidase (hrp) in intraarterial cushions at the origins of intercostal arteries was studied in mice 30 min after intravenous injection of the tracer. hrp is found to be evenly distributed in the extracellular space of the entire arterial wall. basal lamina material is penetrated by the tracer protein. this is not the case with collagenic fibrils, elastic material and intercellular granular deposits which occur regularly in cushions of adult mice but not in those ...	1976	984469
characterization of the chromophores in horseradish peroxidase compounds i and ii using magnetic circular dichroism.		1976	985498
the oxidation of dithiothreitol by peroxidases and oxygen.	horseradish peroxidase (1.11.1.7), lactoperoxidase (1.11.1.7), and the fragment of cytochrome c known as microperoxidase have been shown to catalyze the oxidation of reduced dithiothreitol in an oxygen-consuming reaction. evidence for horseradish peroxidase intermediates compound iii and compound ii has been observed, although ferroperoxidase was not identified during the course of the reaction. the stoichiometry has been extablished as 1 : 1 for oxygen consumed to dithiothreitol oxidized. cyste ...	1976	953035
unit cell dimensions of crystalline horseradish peroxidase.		1976	972399
simultaneous demonstration of horseradish peroxidase and acetylcholinesterase.	simultaneous demonstration of acetylcholinesterase (ache) and horseradish peroxidase (hrp) has been achieved by staining frozen sections with a modified koelle-friedenwald thiocholine method for ache followed directly by the graham-karnovsky procedure for hrp. by using sodium sulfite instead of sodium sulfide in the ache procedure, the final reaction product appears as black ache granules that contrast sharply with the yellowish-brown hrp granules.	1976	19604859
anatomical analysis of ventrolateral thalamic input to primate motor cortex.	1. the origin and topographical organization of input to the arm area of the primate motor cortex from the ventrolateral thalamus were examined using the method of retrograde transport of horseradish peroxidase (hrp). 2. a thin, continuous slab of labeled neurons was found in the ventrolateral thalamus followingmultiple injections of hrp into the arm area of the motor cortex. the slab of labeled neurons was flanked, medially and laterally, by groups of unlabeled neurons. 3. the origin of ventrol ...	1976	62039
[catalytic properties and stability of horseradish peroxidase immobilized in polyacrylamide gel].	effect of polyacrylamide (paa) gel on properties of horseradish peroxidase, immobilized by means of the incorporation into paa gel is studied. catalytic properties of immobilized enzyme are studied. km value and ph-dependency of the enzyme activity are found to be close to those of soluble enzyme, kcat value is 3 times lower at ph 7.0. ph-stability of immobilized peroxidase at 20 degrees c and thermostability of soluble and immobilized peroxidases at ph 7.0 within the temperature range from 20 t ...	1976	10016
increased permeability of cerebral vessels to horseradish peroxidase induced by ischemia in mongolian gerbils.	cerebral ischemia was induced by occlusion of the left common carotic artery in adult mongolian gerbils. the period of occlusion was 3, 6, or 18 h. horseradish peroxidase (hrp) was intravenously injected in animals with clear neurological signs 1 h release of the clip. the hrp was allowed to circulate for 5 min. fixation was carried out by perfusion with aldehydes. tissue, incubated for peroxidatic activity, from the left side of the brain was treated for electron microscopy. during the postisch ...	1976	961382
resonance raman evidence for fe (iv) in compound ii of horseradish peroxidase.		1976	962956
concanavalin a-horseradish peroxidase bridge staining of alpha-1 glycoproteins separated by isoelectric focusing on polyacrylamide gel.	the coomassie blue protein stain and the periodic acid-schiff stain for glycoproteins are compared to a new method of staining glycoproteins resolved electrophoretically. the method utilizes a concanavalin a-horseradish peroxidase sequence to visualize selectively glycoproteins with terminal or internal mannose or terminal n-acetylglucosamine. the method applied to characterization of m and z allele products of alpha-l-antitrypsins separated by isoelectric focusing of polyacrylamide gels slabs h ...	1976	60439
substituent effect on the oxidation of phenols and aromatic amines by horseradish peroxidase compound i.	a stopped-flow kinetic study shows that the reduction rate of horseradish peroxidase compound i by phenols and aromatic amines is greatly dependent upon the substituent effect on the benzene ring. morever it has been possible to relate the reduction rate constants of monosubstituted substrates by a linear free-energy relationship (hammett equation). the correlation of log (rate constants) with sigma values (hammett equation) and the absence of correlation with sigma+ values (okamoto-brown equati ...	1976	954758
mechanisms of electron transfer from sulfite to horseradish peroxidase-hydroperoxide compounds.	using a rapid-scan spectrophotometer equipped with a stopped-flow apparatus, reactions of sulfite with compounds i and ii of two horseradish peroxidase isoenzymes a and c were investigated. the direct two-electron reduction of peroxidase compound i by sulfite occurred at acidic ph but the mechanism gradually changed to the two-step reduction with the intermediate formation of compound ii as the ph increased. the ph at which the one- and two-electron changes occurred at the same speed was 4.5 for ...	1976	8081
synaptic activity of frog retinal photoreceptors. a peroxidase uptake study.	the uptake of horseradish peroxidase (hrp) into membranous structures, detectable by light and electron microscopy, is used here to monitor the synaptic activity of photoreceptors of isolated frog retinas maintained in the dark or under various illumination conditions. the major findings are: (a) neurotransmission from photoreceptor terminals seems to involve the same types of endocytic membrane-retrieval processes that occur at other nerve terminals. presumably, the endocytic processes compensa ...	1976	1084350
peroxidase uptake by photoreceptor terminals of the skate retina.	the photoreceptors of dark-adapted skate retinas bathed in a ringer solution containing horseradish peroxidase (hrp) incorporate the tracer into membrane-bound compartments within the synaptic terminal of the cell; after 1 or 2 h of incubation, approx. 10-38% of the synaptic vesicles were labeled. the receptors appeared to be functioning normally throughout the incubation period, since electrical potentials of normal amplitude could be elicited in response to dimphotic stimuli. however, it was p ...	1976	932103
the kinetics of formation of horseradish peroxidase compound i by reaction with peroxobenzoic acids. ph and peroxo acid substituent effects.	1. the kinetics of formation of horseradish peroxidase compound i were studied by using peroxobenzoic acid and ten substituted peroxobenzoic acids as substrates. kinetic data for the formation of compound i with h2o2 and for the reaction of deuteroferrihaem with h2o2 and peroxobenzoic acids, to form a peroxidatically active intermediate, are included for comparison. 2. the observed second-order rate constants for the formation of compound i with peroxobenzoic acids decrease with increasing ph, i ...	1976	9067
immunocytochemical localization of nerve growth factor (ngf) in the submandibular gland of adult mice by light and electron microscopy.	nerve growth factor (ngf) was localized in the submandibular gland of adult male mice by a direct immunocytochemical method using highly purified antibodies against ngf coupled to horseradish peroxidase. in light microscopic sections the reaction product was entirely confined to the cells of the secretory tubules. the acinar part of the gland was free of reaction product. this finding was confirmed by electron microscopy. within the cells ngf was localized exclusively in the apical secretory gra ...	1976	949726
monosynaptic inputs to caudate neurons identified by intracellular injection of horseradish peroxidase.		1976	1276937
organization of the tectofugal visual pathway in the pigeon: a retrograde transport study.	in birds, superficial laminae of the optic tectum receive a massive retinal input; the tectum in turn projects upon the nucleus rotundus thalami, which then sends its efferents to the ectostriatal core of the telencephalon. to examine the detailed organization of this principal ascending visual pathway, small injections of the marker horseradish peroxidase (hrp) were placed in various sites throughout the ectostriatum (e) or nucleus rotundus (rt) in pigeons. analysis of the resulting patterns of ...	1976	1270632
descending pathways from the brain stem to the spinal cord in some reptiles. i. origin.	in the present study of the origin of the pathways descending from the brain stem to the spinal cord has been investigated in the reptiles testudo hermanni, pseudemys scripta elegans, tupinambis nigropunctatus and python reticulatus. these reptiles, using highly different types of progression, have been selected, because fundamental variations in the organization of the central motor apparatus are to be expected. the origin of the descending pathways has been demonstrated by recording the occurr ...	1976	1270628
choroid plexus absorption of horseradish peroxidase from the cerebral ventricles.		1976	933261
trans-glial channel-facilitated translocation of tracer protein across ventral nerve root sheaths of crayfish.	trans-glial channels, which traverse the multilamellate glial sheath of crayfish nerves, are easily recognized in freeze-fracture preparations. their structure and position in the glial layers of the sheath strongly supports the suggestion that they serve to facilitate rapid movement of molecules and fluids from outside the sheath to the surface of axons contained within. segments of ventral ganglion nerve roots, which were ligated at their free ends, were immersed in crayfish ringer solution co ...	1976	1276892
striatal afferent connections in the turtle (chrysemys picta) as revealed by retrograde axonal transport of horseradish peroxidase.	horseradish peroxidase (hrp, 30% solution, 0.1-0.3 mul, 72 h) was injected unilaterally into the basal striatum (str) and the dorsal ventricular ridge (dvr) of adult turtles (chrysemys picta) in order to demonstrate the cells of origin of some afferents to these telencephalic structures. after selective str injection, hrp-labeled cells were visualized in the dorsal thalamus and midbrain tegmentum, ipsilaterally. at thalamic level, hrp-positive neurons were located around nucleus rotundus, i.e., ...	1976	1276890
infrared spectroscopic studies of carbonyl horseradish peroxidases.	infrared difference spectra, feiiico vs. feiii of horseradish peroxidase isoenzymes a2 and c were recorded from 2000 to 1800 cm-1. under alkaline conditions, ph 9, both isoenzymes exhibit two co stretching bands, at 1938 and 1925 cm-1 for a2 and at 1933 and 1929 cm-1 for c. as the ph is lowered the low-frequency band for each isoenzyme decreases in intensity with a concommitant appearance and increase in intensity of a band at 1906 and 1905 cm-1 for the a2 and c isoenzymes, respectively. these c ...	1976	1276134
the purification of horseradish peroxidase by affinity chromatography on sepharose--bound concanavalin a1,2.		1976	942057
trans-glial channels in ventral nerve roots of crayfish.	the sheath around the roots of the sixth abdominal ganglion in the ventral nerve cord of the crayfish consists of concentric layers of thin glial processes alternating with wide clefts containing filamentous connective tissue. regions of each glial lamella are perforated by single, short, tubular channels: the trans-glial channels. in thin plastic sections examined in the electron microscope, the channels appear as slits that are 240 a wide and 450-550 a long which traverse glial lamellae less t ...	1976	1270620
binding of hydrogen donors to horseradish peroxidase: a spectroscopic study.		1976	938055
magnetic circular dichroism studies on horseradish peroxidase.	magnetic circular dichroism (mcd) spectra were observed for native (fe(iii)) horseradish peroxidase (peroxidase, ec 1.11.1.7), its alkaline form and fluoro- and cyano-derivatives, and also for reduced (fe(ii)) horseradish peroxidase and its carbonmonoxy-- and cyano- derivatives. mcd spectra were obtained for the cyano derivative of fe(iii) horseradish peroxidase, and reduced horseradish peroxidase and its carbonmonoxy- derivative nearly identical with those for the respective myoglobin derivativ ...	1976	1268223
the isolation and characterization of the glycopeptides from horseradish peroxidase isoenzyme c.	the purity of horseradish peroxidase isoenzyme c was demonstrated using isoelectric focusing, polyacrylamide gel electrophoresis at two ph values and cellulose acetate electrophoresis at two ph values. the glycopeptides obtained upon trypsin digestion were isolated using the plant lectin, concanavalin a, and were resolved using paper electrophoresis. the carbohydrate content of the native peroxidase was 86% accounted for by the carbohydrate content of the glycopeptides thus suggesting little los ...	1976	1268213
heme-linked proton dissociation of carbon monoxide complexes of myoglobin and peroxidase.	it was found from spectrophotometric titration and proton balance measurement that the pka value of a heme-linked protonation group of horseradish ferro-peroxidase c (donor:h2o2 oxidoreductase, ec 1.11.1.7) shifted from 7.25 to 8.25 upon combination with co. the spectrophotometric titration experiment with myoglobin also revealed the presence of a heme-linked protonation group, the pka value being 5.57 in myoglobin and 5.67 in the co-myoglobin complex. it was concluded that the distinct shift of ...	1976	5132
the extravascular channel system in the rostral pituitary of mugil cephalus (teleostei) as revealed by use of horseradish peroxidase.	the interlobular spaces, separating the neuro- and the adenohypophysis can be visualized in the pituitary of the grey mullet by using the horseradish peroxidase technique. on the one hand, these spaces communicate with the pericapillary spaces. on the other, they are linked with the pericellular spaces in the adenohypophysis and the periaxonal spaces in the neurohypophysis, thus providing a pathway for the exchange of metabolites and hormones between blood system, epithelial cells and the termin ...	1976	1268912
horseradish peroxidase/hydrogen peroxide-catalyzed oxidation of the carcinogen n-hydroxy-n-acetyl-2-aminofluorene as effected by cyanide and ascorbate.	horseradish peroxidase and h2o2 mediate n-hydroxy-n-acetyl-2-aminofluorene (n-oh-aaf) conversion into two more potent carcinogens, 2-nitrosofluorene and n-acetoxy-n-acetyl-2-aminofluorene. optical studies of this system indicate that horseradish peroxidase is operating as a peroxidase with n-oh-aaf as the electron donor. our studies confirm the earlier finding that 2-nitrosofluorene and n-acetoxy-n-acetyl-2-aminofluorene are the products of the type ii enzyme-mediated oxidation of n-oh-aaf, but ...	1976	1260768
leukocyte-platelet interaction. release of hydrogen peroxide by granulocytes as a modulator of platelet reactions.	because of the many potent biological capabilities of the blood granulocytes, and their contact with platelets in various physiologic and pathologic states, a possible interaction between granulocytes and platelets was investigated. platelets were purified by gel filtration and via a dialysis membrane were separated from suspensions of autologous granulocytes prepared by dextran sedimentation and resuspended in modified tyrode's buffer. after 20 min at 37 degrees c platelet aggregation was shown ...	1976	947961
uptake of horseradish peroxidase during the cell cycle of hepatoma cells in culture.		1976	71071
entry of peroxidase into neurons of the central and peripheral nervous systems from extracerebral and cerebral blood.	autonomic preganglionic, sensory, and lower motoneuron perikarya within the central nervous system, as well as cell bodies with axons projecting to the circumventricular organs, are retrogradely labeled with horseradish peroxidase (hrp) delivered to their axon terminals by cerebral and extracerebral blood. subsequent to vascular injection of hrp into mice, blood-borne peroxidase passes across permeable vessels in muscle, ganglia, and in all circumventricular organs except for the subcommissural ...	1976	57126
a somatotopic and functional representation of the respiratory muscles in the trigeminal and facial motor nuclei of the carp (cyprinus carpio l.).	the place of the cells innervating six most important respiratory muscles in the carp (cyprinus carpio l.) were determined by means of the horseradish peroxidase technique. it was found that these muscles are represented in the rostral and caudal subdivisions of the trigeminal and facial motor nuclei. all connections were ipsilateral. the distribution of the various cell populations over the different subnuclei has been related to the function of the respective muscles in the respiratory cycle. ...	1976	1262554
injection of horseradish peroxidase solution in calibrated volumes (nanolitre) into the spinal cord.	a method for the injection of horseradish peroxidase (hrp) into the spinal cord (sc) is described, based on the principle of fluid expansion upon application of heat. hrp solution in the tip of a glass micropipette (tip diameter 10-15 microm) was extruded when the sealed stem of the pipette, containing distilled water, was warmed. the pipette was calibrated (temperature/volume relation) before it was inserted into the sc and the injection of hrp was made according to the calibration curve. using ...	1976	19604813
effects of 2,4-substituents of deuteroheme upon the stability of the oxy-form and compound i of horseradish peoxidases.		1976	1259443
hydroxylation of p-coumaric acid by horseradish peroxidase. the role of superoxide and hydroxyl radicals.	1. in the presence of dihydroxyfumarate, horseradish peroxidase catalyses the conversion of p-coumaric acid into caffeic acid at ph 6. this hydroxylation is completely inhibited by superoxide dismutase. 2. dihydroxyfumarate cannot be replaced by ascorbate h2o2, nadh, cysteine or sulphite. peroxidase can be replaced by high (10 mm) concentrations of feso4, but this reaction is almost unaffected by superoxide dismutase. 3. hydroxylation by the peroxidase/dihydroxyfumarate system is completely inhi ...	1976	942369
gustatory pathways in the bullhead catfish. 1. connections of the anterior ganglion.	the central projections of the external gustatory system in bullhead catfish were examined using orthograde degeneration and retrograde transport of horseradish peroxidase (hrp) techniques. both large and small cells were observed in the anterior ganglion which contains a mixture of elements from the trigeminal, facial and anterior lateral line nerves. some of the large cells on the lateral margin of the ganglion were found to belong to the lateral line system. no separation of trigeminal and fa ...	1976	1262542
a study of the supposed hydroxylation of tyrosine catalysed by peroxidase.	the claim that peroxidase (rather than tyrosinase) is the enzyme responsible for the conversion of tyrosine into dopa (3,4-dihydroxyphenylalanine) in melanogenesis was investigated. the spectral changes that occurred during the action of horseradish peroxidase in the presence of h2o2 on dopa, tyrosine and mixtures of dopa with tyrosine or other phenolic compounds were studied. the effect of ascorbic acid or dihydroxyfumaric acid on some of these changes was also investigated. no evidence was fou ...	1976	1275895
the porphyrin-sensitized photooxidation of horseradish apoperoxidase.		1976	1259421
in vitro formation of gap junction vesicles.	a method is described that uses trypsin digestion combined with collagenase-hyaluronidase which produces a population of gap junction vesicles. the hexagonal lattice of subunits ("connexons") comprising the gapjunctions appears unaltered by various structural criteria and by buoyant density measurements. the gap junction vesciles are closed by either a single or a double profile of nonjunctional "membrane," which presents a smooth, particle-free fracture face. horseradish peroxidase and cytochro ...	1976	54358
cerebello-olivary fibers: their origin, course and distribution in the north american opossum.	although degeneration techniques suggest that cerebello-olivary fibers are limited in their origin and distribution, horseradish peroxidase and autoradiographic experiments make it clear that they arise within all cerebellar nuclei and project to most, if not all, areas of the contralateral inferior olive. autoradiographic preparations show that cerebello-olivary fibers are highly ordered and suggest that the dentate nucleus projects primarily to the principal olive, the interpositus anterior re ...	1976	1253856
formation of hydrogen peroxide by isolated cell walls from horseradish (armoracia lapathifolia gilib.).	isolated cell-wall suspensions from horseradish in the presence of 5×10(-4) m mncl2 catalyze the production of hydrogen peroxide at the expense of either nadph or nadh. this reaction is inhibited by scavengers of the superoxide free radical ion such as ascorbate or dihydroxyphenols or by superoxide dismutase, and stimulated by monophenols such as p-coumaric acid. on comparison with isolated (commercial) horseradish peroxidase it becomes evident that (a) cell-wall-bound peroxidase(s) is (are) res ...	1976	24424595
the sarcolemma of aplysia smooth muscle in freeze-fracture preparations.	smooth muscle cells in the sheath covering the visceral ganglion of aplysia californica were examined with the techniques of freeze-fracture and conventional electron microscopy. the sarcolemma of these muscle cell invaginates to form myriad caveolae that have an intrinsic marker within their membrane. this intrinsic structure of the caveolar membrane is revealed by freeze-fracture and consists of rows of large particles in the outer half and matching grooves on the complementary inner half of t ...	1976	18631588
electron microscopic study on gut epithelium of the tench (tinca tinca l.) with respect to its absorptive functions.	few morphological differences are seen along the intestinal tract of the adult tench (tinca tinca l.) a stomachless freshwater teleost. however, three segments can be distinguished, when function and structure of enterocytes are studied. the enterocytes of the proximal segment are found to be concerned with dietary lipids absorption. in the cell, absorbed fats are seen in two inclusion bodies: lipid particles and lipid droplets. only lipid particles are involved in direct transport of absorbed f ...	1976	982424
the sarcolemma of aplysia smooth muscle in freeze-fracture preparations.	smooth muscle cells in the sheath covering the visceral ganglion of aplysia californica were examined with the techniques of freeze-fracture and conventional electron microscopy. the sarcolemma of these muscle cell invaginates to form myriad caveolae that have an intrinsic marker within their membrane. this intrinsic structure of the caveolar membrane is revealed by freeze-fracture and consists of rows of large particles in the outer half and matching grooves on the complementary inner half of t ...	1976	941133
peroxidase-catalyzed halogenation.	peroxidase-catalyzed halogenation reactions have been established as being important in the biosynthesis of the hormone thyroxine and in biological defense mechanisms. recently these reactions have been recognized as valuable tools for the study of proteins as well as their arrangement in macromolecular structures. the pathways of peroxidase catalyses can be accommodated within the framework of the classical chance-george mechanism. this implies that the initial steps of the reaction invariably ...	1976	786162
production of the superoxide radical by horseradish peroxidase.		1976	187464
ultrastructural basis for alveolar-capillary permeability to protein.	the intravenous injection into mice of small volumes (less than 0.1 ml) of peroxidatic enzymes of molecular weight of 40 000 daltons or greater results in little if any penetration of these probe molecules into endothelial junctions. the injection of cytochrome c (12 000 daltons), on the other hand, results in the localization of this tracer in some but not all endothelial junctions. when horseradish peroxidase (ec 1.11.1.7) is injected in a large volume of saline (0.5 ml), reaction product is p ...	1976	181220
modification and regeneration of synaptic connections in cultured leech ganglia.	segmental ganglia of the central nervous system of the leech were maintained in culture medium outside the animal for several weeks in order to study the properties of synapses and regeneration by identified sensory and motor nerve cells. a variety of preparations were used, including single ganglia, chains of ganglia and ganglia connected to the areas of skin and muscle that they normally innervate in the animal. (1) for up to 10 weeks after removal from the animal, resting and action potential ...	1976	181202
absorption of free and antibody-conjugated horseradish peroxidase to fixed glial cells in tissue culture, a possible source of error in immunohistochemistry.	incubation of cellular preparations with antibody-conjugated peroxidase result in a very strong unspecific staining, for one electropolar binding of the basic enzymic protein with the cell structures. in immunohistochemical practice, it may represent an evil as import as when dealing with immunofluorescence techniques. this background can to a great extent be avoided by rinsing the preparations in buffer at ph = 6.2.	1976	12639
comparison of function of the distal base between myoglobin and peroxidase.	the heme-linked protonation of a ferrous horseradish peroxidase is assigned to a distal amino acid residue. the conclusion is drawn from analyses of reactions that involve the protonation. unfortunately it is difficult to apply it to the myoglobin case because no reaction has been found which is coupled with the protonation of the distal histidine. it is therefore of special interest to note that the pk value of 5.7 has been assigned to the distal histidine of metmyoglobin from binding kinetics ...	1976	8964
probing axial ligands in ferric haemoproteins: an esr study of myoglobin and horseradish peroxidase in h217o.		1977	202277
the visual cortex of the opossum: the retrograde transport of horseradish peroxidase to the lateral geniculate and lateral posterior nuclei.	the visual cortex of opossum was studied by injecting horseradish peroxidase into the cortex and identifying labeled neurons in the thalamus. the results show that the lateral geniculate nucleus projects to area 17 in a topographical manner: the rostral lateral geniculate is represented in caudal striate cortex, and the dorsal extremity of the lateral geniculate, which probably corresponds to the zero vertical meridian, is represented along the border of area 18. small injections in area 17 prod ...	1977	73403
membrane alterations during cornification of mammalian squamous epithelia: a freeze-fracture, tracer, and thin-section study.	tight junctions (zonulae occludentes) create a pericellular barrier to the diffusion of large molecules in non-keratinizing mammalian epithelia. however, in cornifying epithelia such as the epidermis, the importance of tight-junctional elements versus secreted intercellular lipid for barrier function is uncertain. in an attempt to resolve this question, we compared membrane structure in the stratum granulosum and stratum corneum of epidermis, esophagus, and vagina of newborn and adult humans and ...	1977	339780
circular dichroism studies on native and artificial horseradish peroxidases.		1977	914868
effect of indole-3-acetic acid on the kinetics of horseradish peroxidase catalyzed scopoletin oxidation.	the kinetics of horseradish peroxidase catalyzed of scopoletin oxidation were observed to be sigmoidal. the apparent kms for scopoletin is 0.9 mm. inhibition of scopoletin oxidation by indole-3-acetic acid (iaa) appears to be non competitive. non competitive inhibition by iaa suggests a more significant role of the peroxidase protein matrix in regulating the activity of the heme moiety.	1977	923704
[silymarin--an inhibitor of horseradish peroxidase (author's transl)].	silybin and silychristin non-competitively inhibit the horseradish peroxidase in vitro. silydianin on the other hand is a competitive inhibitor of the enzyme.	1977	923693
nuclear magnetic resonance studies of hemoproteins. acid-alkaline transition, ligand binding characteristics, and structure of the heme environments in horseradish peroxidase.		1977	20945
descending input from the pneumotaxic system to the lateral respiratory nucleus of the medulla. an anatomical study with the horseradish peroxidase technique.		1977	19605040
high resolution of peroxidase-indoleacetic acid oxidase isoenzymes from horseradish by isoelectric focusing.	several improved techniques for isoelectric focusing of isoenzymes in polyacrylamide gel slabs were developed. using these techniques, three commercial sources of horseradish peroxidase were each examined with three commercial sources of carrier ampholytes to determine their respective isoenzyme profiles.a much higher degree of isoenzyme resolution was obtained than reported previously. a composite of all of the various tests gave a total of 42 peroxidase isoenzymes. commercial sources of horser ...	1977	16660185
a sensitive histochemical method for light-and electron-microscopic demonstration of horseradish peroxidase.		1977	915248
a new specific, sensitive and non-carcinogenic reagent for the demonstration of horseradish peroxidase.		1977	72746
fine structural and ultracytochemical studies on the lymphocytes in three types of genetic mucopolysaccharidoses.	lymphocytes from 6 patients with 3 types of genetic mucopolysaccharidoses (hurler's syndrome, hunter's syndrome and morquio's syndrome) contained numberous vacuoles in their cytoplasm. the size of the vacuoles ranged from approximately 300 nm to 750 nm. the percentage of the lymphocytes with vacuoles varied from 10% to 38%. the vacuoles showed acid phosphatase activity, which indicated their lysosommal nature. staining with dialyzed iron solution usually localized acid mucosubstance in the perip ...	1977	412306
differential termination of large-diameter and small-diameter primary afferent fibers in the spinal dorsal gray matter as indicated by labeling with horseradish peroxidase.		1977	19605029
a simple apparatus for the injection of small (nanolitre) volumes of horseradish peroxidase.		1977	19605023
peroxidase and gold complexes of lectins for double labeling of surface-binding sites by electron microscopy.	double labeling experiments were performed for visualization of the binding sites of concanavalin a and anti-ahel (the lectin from helix pomatia). the anti-ahel was labeled with the colloidal gold whereas the membrane bound concanavalin a was demonstrated by an affinity technique using horseradish peroxidase. the two markers used could be clearly distinguished electron microscopically. the specificity of the cell surface double labeling was demonstrated in the control experiments. a topological ...	1977	915241
the nucleus corporis pontobulbaris of the north american opossum.	the nucleus of the pontobulbar body (pbu) in the north american opossum is located, for the most part, adjacent to the motor root of the trigeminal nerve. material prepared by degeneration and autoradiographic methods shows that the pbu receives projections from the facial motor-sensory cortex, red nucleus, spinal cord and cerebellum. the latter fibers probably take origin within the fastigial nucleus. each of the afferent connections ends in a restricuted part of the pbu, but there is considera ...	1977	903428
concanavalin a-binding by cells of the early chick embryo.	the surfaces of cells from the early embryo of the chick were examined using electron microscope techniques for the visualization of concanavalin a-binding sites. horseradish peroxidase and ferritin labelled concanavalin a were used to determine the distribution of the binding sites. all surfaces of the epiblast and hypoblast layers which were accessible to concanavalin a showed the presence of binding sites in stage 1 embryos. the ventral surface of the epiblast showed a high lectin affinity wh ...	1977	561795