Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| the isolation of cdna clones from cucumber (cucumis sativus l.) floral buds coming from plants differing in sex. | in this study, we found flower cdna clones which may be connected with the development of flower sex in cucumber. two pairs of nearly-isogenic lines: gynoecious gy3 (ffmmgg) versus hermaphrodite hgy3 (ffmmgg) and monoecious b10 (ffmmgg) versus gynoecious 2gg (ffmmgg) were used for clone isolation. to obtain differentially-expressed clones, we applied the differential screening method. 454 clones from gy3 and 478 from b10 cdna libraries were isolated. the results of rflp analysis with 56 cdna clo ... | 2003 | 12813576 |
| aflp marker polymorphism in cucumber (cucumis sativus l.) near isogenic lines differing in sex expression. | the aflp technique was used to evaluate the level of polymorphism between two pairs of isogenic cucumber (cucumis sativus l.) lines (nil) differing in flower sex expression. the bsa techniques were also applied to find molecular markers linked to sex determination genes (dominant alleles) in those cucumber lines. sex determination in cucumber is controlled by three main loci f, m and gy. the interaction of these loci is responsible for the formation of the various phenotypes of flowers in respec ... | 2003 | 12813572 |
| nitrate transport across the tonoplast of cucumis sativus l. root cells. | nitrate transport across the tonoplast has been studied using vacuole membranes isolated from cucumber roots grown in nitrate. the addition of no3- ions into the tonoplast with atp-generated transmembrane proton gradient caused the dissipation of delta ph, indicating the no3(-)-induced proton efflux from vesicles. no3(-)-dependent h+ efflux was almost insensitive to the transmembrane electrical potential difference, suggesting the presence of an electroneutral no3-/h+ antiporter in the tonoplast ... | 2003 | 12806781 |
| [use of fish oil enriched with carotenoids isolated from sea cucumbers in the diet therapy of patients with hypertension and ischemic heart disease]. | usage of antisclerotic diet with fish oil and carotinoides in patients with ind and high blood pressure resulted in positive dynamic of clinic manifestation, blood lipid, coagulogramms, antioxygen and immune status. | 2003 | 14870585 |
| dynamic mechanical properties of body-wall dermis in various mechanical states and their implications for the behavior of sea cucumbers. | the dermis of the sea cucumber body wall is a typical catch connective tissue that rapidly changes its mechanical properties in response to various stimuli. dynamic mechanical properties were measured in stiff, standard, and soft states of the sea cucumber actinopyga mauritiana. sinusoidal deformations were applied, either at a constant frequency of 0.1 hz with varying maximum strain of 2%-20% or at a fixed maximum strain of 1.8% with varying frequency of 0.0005-50 hz. the dermis showed viscoela ... | 2003 | 14672981 |
| [effects of sunlight and temperature on ultrastructure and functions of chloroplast of cucumber in solar greenhouse]. | studies on the effects of sunlight and temperature on the ultrastructure and functions of leaf chloroplasts of cucumber (cucumis sativus l.) in solar greenhouse showed that the size of cells, chloroplasts, and starch grains and the number of chloroplast, grana, grana lamella and grana thickness of leaf chloroplast of cucumber increased with lowering leaf position. the microstructure of leaves and the ultrastructure of chloroplast of cucumber leaves were different because of the difference of tem ... | 2003 | 14655360 |
| effectiveness of gf-120 fruit fly bait spray applied to border area plants for control of melon flies (diptera: tephritidae). | in a field study in hawaii, color-marked protein-deprived and protein-fed female melon flies, bactrocera cucurbitae coquillett, were released within canopies of unsprayed sorghum plants (a nonhost of melon flies) outside of a border area of unsprayed or bait-sprayed sorghum plants or open space that surrounded cucumbers, a favored host of melon flies. application of bait spray to sorghum or sugarcane surrounding host plants of melon flies is a common practice for melon fly control in hawaii. gf- ... | 2003 | 14650522 |
| ethylene stimulates endoreduplication but inhibits cytokinesis in cucumber hypocotyl epidermis. | the effects of ethylene on cell division are generally considered inhibitory. in this study, we demonstrate that transient ethylene exposure, while suppressing cytokinesis, stimulates dna synthesis. we monitored dna synthesis and cytokinesis in the epidermis of cucumber (cucumis sativus) hypocotyls, an organ whose post-germination development involves strictly limited cell division. during exposure to ethylene, dna synthesis, assessed by the incorporation of the thymidine homolog 5-bromo-2'-deox ... | 2003 | 14645725 |
| possible involvement of leaf gibberellins in the clock-controlled expression of xsp30, a gene encoding a xylem sap lectin, in cucumber roots. | root-produced organic compounds in xylem sap, such as hormones and amino acids, are known to be important in plant development. recently, biochemical approaches have revealed the identities of several xylem sap proteins, but the biological functions and the regulation of the production of these proteins are not fully understood. xylem sap protein 30 kd (xsp30), which is specifically expressed in the roots of cucumber (cucumis sativus), encodes a lectin and is hypothesized as affecting the develo ... | 2003 | 14605217 |
| a gene for a class ii dna photolyase from oryza sativa: cloning of the cdna by dilution-amplification. | ultraviolet radiation induces the formation of two classes of photoproducts in dna-the cyclobutane pyrimidine dimer (cpd) and the pyrimidine [6-4] pyrimidone photoproduct (6-4 product). many organisms produce enzymes, termed photolyases, which specifically bind to these lesions and split them via a uv-a/blue light-dependent mechanism, thereby reversing the damage. these photolyases are specific for either cpds or 6-4 products. two classes of photolyases (class i and class ii) repair cpds. a gene ... | 2003 | 12764611 |
| subcellular localization of two types of ferrochelatase in cucumber. | it is widely believed that ferrochelatase (protoheme ferrolyase, ec 4.99.1.1), which catalyzes the insertion of ferrous ion into protoporphyrin ix to form protoheme, exists in both plastids and mitochondria of higher plants. by in vitro import assay with isolated pea (pisum sativum l.) organelles, it has been proposed that one of two isoforms of ferrochelatase (type 1) is dual-targeted into both plastids and mitochondria, and functions for heme biosynthesis in the both organelles. recently, howe ... | 2003 | 12905021 |
| csagp1, a gibberellin-responsive gene from cucumber hypocotyls, encodes a classical arabinogalactan protein and is involved in stem elongation. | fluorescence differential display was used to isolate the gibberellin (ga)-responsive gene, csagp1, from cucumber (cucumis sativus) hypocotyls. a sequence analysis of csagp1 indicated that the gene putatively encodes a "classical" arabinogalactan protein (agp) in cucumber. transgenic tobacco (nicotiana tabacum) plants overexpressing csagp1 under the control of the cauliflower mosaic virus 35s promoter produced a y(betaglc)(3)-reactive proteoglycan in addition to agps present in wild-type tobacco ... | 2003 | 12644694 |
| the hemolytic and physiological activities of mixtures of some phenoxy and organophosphorous herbicides. | experiments were performed investigating the potential to improve the biological activity of some phenoxy and organophosphorous compounds by using them in binary mixtures. the compounds were: 2,4-dichlorophenoxyacetic acid (1) and its sodium salt (2), dibutyl 1-butylamino-1-cyclohexanephosphonate (3) and diethyl 9-butylamino-9-fluorenephosphonate (4), all widely used as herbicides. there were two test methods: the inhibition of cucumber (cucumis sativus) growth induced by one single herbicide or ... | 2003 | 12655357 |
| "chitin-specific" peroxidases in plants. | the activity of various plant peroxidases and the ability of their individual isoforms to bind chitin was studied. some increase in peroxidase activity was observed in crude extracts in the presence of chitin. activated peroxidases of some species fell in the fraction not sorbed on chitin and those of other species can bind chitin. only anionic isoperoxidases from oat (avena sativa), rice (oryza sativa), horseradish (armoracia rusticana), garden radish (raphanus sativus var. radicula), peanut (a ... | 2003 | 12693984 |
| wound-responsive cis-element in the 5'-upstream region of cucumber ascorbate oxidase gene. | the cucumber (cucumis sativas) aao1 gene (former name, aso1) encodes an ascorbate oxidase that catalyzes the oxidation by molecular oxygen of ascorbic acid to dehydroascorbate. csaao1 mrna concentrations rose rapidly after mechanical wounding of cucumbers. to study the wound-responsive expression of csaao1 in detail, we examined transgenic tobacco plants harboring a csaao1 promoter-beta-glucuronidase fusion gene. csaao1 promoter activity in leaves of the tobacco was induced by wounding. analysis ... | 2003 | 12728985 |
| studies on structure-function relationships of indolepyruvate decarboxylase from enterobacter cloacae, a key enzyme of the indole acetic acid pathway. | enterobacter cloacae, isolated from the rhizosphere of cucumbers, produces large amounts of indole-3-acetic acid. indolepyruvate decarboxylase, the key enzyme in the biosynthetic pathway of indole-3-acetic acid, catalyses the formation of indole-3-acetaldehyde and carbon dioxide from indole-3-pyruvic acid. the enzyme requires the cofactors thiamine diphosphate and magnesium ions for catalytic activity. recombinant indolepyruvate decarboxylase was purified from the host escherichia coli strain jm ... | 2003 | 12752452 |
| investigation of an outbreak of e. coli o157 infections associated with a trip to france of schoolchildren from somerset, england. | a retrospective cohort study was conducted after 2 confirmed and 8 probable cases of vtec 0157 occurred in a schoolgroup from somerset following a trip to france. the strain of the 2 confirmed cases was vero-cytotoxin type 2, phage type 34. despite its low statistical power due to small numbers, this study suggested the most likely vehicle of infection was cucumber salad (crude rr=4.3, 95%ci [0.6-29], adjusted or=107, 95%ci indeterminate, p=0.08). the cucumbers were purchased from belgium but it ... | 2003 | 12764237 |
| detection of segmental internal fat by bioelectrical impedance analysis in a biological phantom. | quantification of internal adipose tissue such as visceral adipose tissue currently relies on expensive, cross-sectional imaging modalities. the purpose of this study was to test the hypothesis that surface impedance, determined by bioimpedance analysis, might be used to predict regional internal fat content change in a phantom model. | 2003 | 12781856 |
| [effect of extractive substances and total glycoside fraction from panax ginseng c. a. meyer on root growth of cucumis sativus l. seedlings]. | we studied the effect of extractive substances (es) and total glycoside fraction (tgf) from panax ginseng on plant cell growth. the seeds of cucumber cucumis sativus l., kit variety, were used for the biological assay. the tested substances inhibited the primary root growth in cucumis sativus seedlings. actively growing seedlings were most sensitive to this effect. | 2003 | 12816069 |
| the development and evaluation of consensus chloroplast primer pairs that possess highly variable sequence regions in a diverse array of plant taxa. | although universal or consensus chloroplast primers are available, they are limited by their number and genomic distribution. therefore, a set of consensus chloroplast primer pairs for simple sequence repeats (ccssrs) analysis was constructed from tobacco (nicotiana tabacum l.) chloroplast sequences. these were then tested for their general utility in the genetic analysis of a diverse array of plant taxa. in order to increase the number of ccssrs beyond that previously reported, the target seque ... | 2003 | 12827249 |
| identification and biochemical characterization of plant acylamino acid-releasing enzyme. | plant acylamino acid-releasing enzyme (aare) catalyzing the n-terminal hydrolysis of n(alpha)-acylpeptides to release n(alpha)-acylated amino acids, was biochemically characterized using recombinant and native aares. a cdna encoding a deduced arabidopsis thaliana aare (ataare) was cloned and sequenced. the deduced amino acid sequence encoded a 764 amino acid protein of 83.9 kda, which was 31.8% identical with that of rat aare. in particular, the proposed catalytic residues (ser, asp, and his) of ... | 2003 | 12966075 |
| joint effects of elevated levels of ultraviolet-b radiation, carbon dioxide and ozone on plants. | there is growing interest regarding the joint effects of elevated levels of surface ultraviolet b (uv-b) radiation, carbon dioxide (co2) and ozone (o3) on plants. our current knowledge of this subject is too limited to draw any specific conclusions, although one might state that such effects are likely to be highly species dependent and may be more than additive, additive or less than additive. there are a number of uncertainties associated with the experimental protocols used and the conclusion ... | 2003 | 14743861 |
| melon resistance to cucurbit yellow stunting disorder virus is characterized by reduced virus accumulation. | abstract the pattern of accumulation of cucurbit yellow stunting disorder virus (cysdv; genus crinivirus, family closteroviridae) rna has been analyzed in several cucurbit accessions. in susceptible accessions of melon (cucumis melo), cucumber (cucumis sativus), marrow (cucurbita maxima), and squash (cucurbita pepo), cysdv rna accumulation peaked during the first to second week postinoculation in the first to third leaf above the inoculated one; younger leaves showed very low or undetectable lev ... | 2003 | 18943165 |
| linkage map of cucumis melo including phenotypic traits and sequence-characterized genes. | a new linkage map of cucumis melo, derived from the f2 progeny of a cross between pi 414723 and c. melo 'topmark' is presented. the map spans a total of 1421 cm and includes 179 points consisting of random amplified polymorphic dna (rapd), amplified fragment length polymorphism (aflp), inter-simple sequence repeats (issrs), simple sequence repeats (ssrs), and restriction fragment length polymorphism (rflp) markers. the map also includes an aphid resistance trait (vat) and the sex type gene, andr ... | 2003 | 14608393 |
| development of microsatellite markers from an enriched genomic library for genetic analysis of melon (cucumis melo l.). | despite the great advances in genomic technology observed in several crop species, the availability of molecular tools such as microsatellite markers has been limited in melon (cucumis melo l.) and cucurbit species. the development of microsatellite markers will have a major impact on genetic analysis and breeding of melon, especially on the generation of marker saturated genetic maps and implementation of marker assisted breeding programs. genomic microsatellite enriched libraries can be an eff ... | 2004 | 15149552 |
| a pisum sativum glyoxysomal malate dehydrogenase induced by cadmium exposure. | the glyoxysomal malate dehydrogenase (gmdh) catalyses the formation of oxaloacetate from malate during beta-oxidation of fatty acids in the glyoxysome. a partial pisum sativum l. (cv. greenfeast) cdna was first isolated from a suppression subtractive hybridisation cdna library obtained from heavy metal stressed plants. the full length cdna was then isolated by rapid amplification of cdna ends. the translated sequence showed strong similarity to cucumis sativus and citrullus lanatus gmdh includin ... | 2004 | 15497444 |
| screening of lactic acid bacteria from fermented vegetables by carbohydrate profiling and pcr-elisa. | the aim of this study was to identify potential souring agents, isolated from fermented plant material, by api 50 chl assay and a molecular method based on polymerase chain reaction and colorimetric hybridization (pcr-elisa). | 2004 | 15482435 |
| degradation of acyl-homoserine lactone molecules by acinetobacter sp. strain c1010. | a bacterium c1010, isolated from the rhizospheres of cucumbers in fields in korea, degraded the microbial quorum-sensing molecules, hexanoyl homoserine lactone (hhsl), and octadecanoyl homoserine lactone (ohsl). morphological characteristics and 16s rrna sequence analysis identified c1010 as acinetobacter sp. strain c1010. this strain was able to degrade the acyl-homoserine lactones (ahls) produced by the biocontrol bacterium, pseudomonas chlororaphis o6, and a phytopathogenic bacterium, burkhol ... | 2004 | 15644910 |
| reactions of some cucurbitaceous species tozucchini yellow mosaic virus (zymv). | zucchini yellow mosaic virus (zymv) is a widespread serious pathogen of cucurbitaceous plants. zymv was first detected in hungary in 1995. since then it has become one of the most dangerous viruses of the cucurbitaceae family causing serious epidemics. the virus has many hosts, which - particularly perennial ones - may play important role as virus reservoirs and infection sources in virus epidemiology. on the other hand wild weed species maybe sources of resistance to viruses. our research was c ... | 2004 | 15756830 |
| the composting potential of different organic solid wastes: experience from the island of crete. | for the past 20 years, the national foundation for agricultural research in crete and the school of agricultural technology of the technological and educational institute of crete have been involved in a number of research and development activities, related to the production and evaluation of compost derived from a variety of local solid, mainly agricultural organic wastes. materials such as olive press cake, olive tree leaves (otl) and branches, vine branches (vb), pressed grape skins (pgs), p ... | 2004 | 14680891 |
| cloning, expression, and purification of glyoxysomal 3-oxoacyl-coa thiolase from sunflower cotyledons. | the glyoxysomal beta-oxidation system in sunflower (helianthus annuus l.) cotyledons is distinguished by the coexistence of two different thiolase isoforms, thiolase i and ii. so far, this phenomenon has only been described for glyoxysomes from sunflower cotyledons. thiolase i (acetoacetyl-coa thiolase, ec 2.3.1.9) recognizes acetoacetyl-coa only, while thiolase ii (3-oxoacyl-coa thiolase, ec 2.3.1.16) exhibits a more broad substrate specificity towards 3-oxoacyl-coa esters of different chain le ... | 2004 | 14680958 |
| ntzip antisense plants show reduced chlorophyll levels. | we have isolated and characterized a new photosynthetic tissue-specific gene ntzip (nicotiana tabacum leucine zipper) from tobacco (n. tabacum). its deduced amino acid sequence has two highly conserved regions, leucine zipper and [ex(n)dexrh](2) motifs, which are related to the gene's biochemical functions. ntzip was expressed in leaves and stems, but was not detected in roots or flowers, suggesting that its physiological functions might be associated with photosynthesis. northern blot analysis ... | 2004 | 15120117 |
| acquisition of an agrobacterium ri plasmid and pathogenicity by other alpha-proteobacteria in cucumber and tomato crops affected by root mat. | root mat of cucumbers and tomatoes has previously been shown to be caused by agrobacterium radiobacter strains harboring a root-inducing ri plasmid (pri). nine other pri-harboring alpha-proteobacteria have subsequently been isolated from root mat-infected crops. fatty acid profiling and partial 16s rrna sequence analysis identified three of these strains as being in the genus ochrobactrum, five as being in the genus rhizobium, and one as being in the genus sinorhizobium: an in vitro pathogenicit ... | 2004 | 15128532 |
| preharvest evaluation of coliforms, escherichia coli, salmonella, and escherichia coli o157:h7 in organic and conventional produce grown by minnesota farmers. | microbiological analyses of fresh fruits and vegetables produced by organic and conventional farmers in minnesota were conducted to determine the coliform count and the prevalence of escherichia coli, salmonella, and e. coli o157:h7. a total of 476 and 129 produce samples were collected from 32 organic and 8 conventional farms, respectively. the samples included tomatoes, leafy greens, lettuce, green peppers, cabbage, cucumbers, broccoli, strawberries, apples, and seven other types of produce. t ... | 2004 | 15151224 |
| identification and characterization of proteins that interact with the carboxy terminus of poly(a)-binding protein and inhibit translation in vitro. | poly(a)-binding proteins (pabps) are multifunctional proteins that play important roles in mrna stability and protein translation. two cucumber ( cucumis sativus l.) proteins, pci6 (pabp-ct-interacting) and pci243 were identified based on ability to interact with the carboxy terminus (ct) of pabp in yeast two-hybrid and in vitro binding assays. pci6 and pci243 share a conserved amino acid domain (sxlnpnapxfxp) in common with human pabp-ct interactors, and with arabidopsis erd15 (early-responsive ... | 2004 | 15159636 |
| tobacco pr-2d promoter is induced in transgenic cucumber in response to biotic and abiotic stimuli. | the pr-2d promoter/uida (gus) gene construct was introduced into the cucumber (cucumis sativus l.) genome and several transgenic lines were produced. activation of the pr-2d promoter was investigated in these plants in response to inoculation with fungal pathogens and after salicylic acid (sa) or cold treatments. treatment with exogenous sa increased gus activity 2 to 11 fold over that of the control. endogenous sa and its conjugate salicylic acid glucoside (sag) rose in parallel after inoculati ... | 2004 | 15202719 |
| application of toxkit microbiotests for toxicity assessment in soil and compost. | the potential of toxkit microbiotests to detect and analyze pollution in agricultural soil and the quality of compost was studied. the toxicity tests used included seed germination biotests using cress salad (lepidum sativum l.), tomato (lycopersicum esculentum l.), and cucumber (cucumis sativus l.), and the toxkit microbiotests included those with microalgae (selenastrum capricornutum), protozoa (tetrahymena thermophila), crustaceans (daphnia magna, thamnocephalus platyurus, and heterocypris in ... | 2004 | 15269896 |
| comparative mapping of zymv resistances in cucumber (cucumis sativus l.) and melon (cucumis melo l.). | zucchini yellow mosaic virus (zymv) routinely causes significant losses in cucumber ( cucumis sativus l.) and melon ( cucumis melo l.). zymv resistances from the cucumber population 'tmg1' and the melon plant introduction (pi) 414723 show different modes of inheritance and their genetic relationships are unknown. we used molecular markers tightly linked to zymv resistances from cucumber and melon for comparative mapping. a 5-kb genomic region (ycz-5) cosegregating with the zym locus of cucumber ... | 2004 | 15340688 |
| [spread of bacteria of the genus acinetobacter in the hydrobios of the bay of peter the great, the sea of japan]. | the analysis of the spread of bacteria of the genus acinetobacter among the invertebrate animals of the bay of peter the great (the sea of japan): in bivalved mollusks, sea cucumbers and sea urchins acinetobacter bacteria were detected more often than in cushion stars and crustaceous animals. the biological properties of 45 isolated bacteria were studied with the use of a wide variety of tests. the study revealed that 9% of acinetobacter cultures isolated from marine animals had pronounced beta- ... | 2004 | 15346960 |
| mosaic (msc) cucumbers regenerated from independent cell cultures possess different mitochondrial rearrangements. | passage of the highly inbred cucumber ( cucumis sativus l.) line b through cell culture produces progenies with paternally transmitted, mosaic (msc) phenotypes. because the mitochondrial genome of cucumber shows paternal transmission, we evaluated for structural polymorphisms by hybridizing cosmids spanning the entire mitochondrial genome of arabidopsis thaliana l. to dna-gel blots of four independently generated msc and four wild-type cucumbers. polymorphisms were identified by cosmids carrying ... | 2004 | 14586555 |
| characterization of the solution reactivity of a basic heme peroxidase from cucumis sativus. | a basic heme peroxidase has been isolated from cucumber (cucumis sativus) peelings and characterized through electronic and (1)h nmr spectra from ph 3 to 11. the protein, as isolated, contains a high-spin ferriheme which in the low ph region is sensitive to two acid-base equilibria with apparent pk(a) values of approximately 5 and 3.6, assigned to the distal histidine and to a heme propionate, respectively. at high ph, a new low-spin species develops with an apparent pk(a) of 11, likely due to t ... | 2004 | 15001396 |
| xylem sap protein composition is conserved among different plant species. | xylem sap from broccoli (brassica oleracea l. cv. calabrais), rape (brassica napus l. cv. drakkar), pumpkin (cucurbita maxima duch. cv. gelber zentner) and cucumber (cucumis sativus l. cv. hoffmanns giganta) was collected by root pressure exudation from the surface of cut stems of healthy, adult plants. total protein concentrations were in the range of 100 microg ml(-1). one-dimensional gel electrophoresis (sds-page) resulted in 10-20 visible protein bands in a molecular mass range from 10 to 10 ... | 2004 | 15064951 |
| phytotoxins from the leaves of ruta graveolens. | bioassay-guided fractionation of the ethyl acetate extract of ruta graveolens (common rue) leaves led to the isolation of the furanocoumarins 5-methoxypsoralen (5-mop), 8-methoxypsoralen (8-mop), and the quinolone alkaloid graveoline as phytotoxic constituents. graveoline and 8-mop substantially inhibited growth of lactuca sativa (lettuce) seedlings and reduced chlorophyll content at 100 microm; this effect was not due to a direct effect on chlorophyll synthesis. radical growth of l. sativa was ... | 2004 | 15161195 |
| soil ecotoxicity assessment using cadmium sensitive plants. | four crop plant species (sweet corn, zea may; wheat, triticum aestivum; cucumber, cucumis sativus; and sorghum, sorghum bicolor) were tested to assess an ecotoxicity in cadmium-amended soils. the measurement endpoints used were seed germination and seedling growth (shoot and root). the presence of cadmium decreased the seedling growth. the medium effective concentration values (ec50) for shoot or root growth were calculated by the trimmed spearman-karber method. due to the greater accumulation o ... | 2004 | 14553991 |
| cucumis monosomic alien addition lines: morphological, cytological, and genotypic analyses. | cucumis hystrix chakr. (hh, 2n=24), a wild relative of the cultivated cucumber, possesses several potentially valuable disease-resistance and abiotic stress-tolerance traits for cucumber ( c. sativus l., cc, 2n=14) improvement. numerous attempts have been made to transfer desirable traits since the successful interspecific hybridization between c. hystrix and c. sativus, one of which resulted in the production of an allotriploid (hcc, 2n=26: one genome of c. hystrix and two of c. sativus). when ... | 2004 | 14666371 |
| enhanced rates of p700(+) dark-reduction in leaves of cucumis sativus l photoinhibited at chilling temperature. | the changes in electron transport within photosystem i (psi) were studied in detached leaves of cucumis sativus l. during the course of irradiation with moderate white light (300 micromol photons m(-2) s(-1)) at 4 degrees c. when intact leaves were exposed to the combination of moderate light and low temperature, the amplitude of far-red light-induced p700 absorbance changes at 820 nm (deltaa(820)), a relative measure of psi, progressively decreased as the light treatment time increased. almost ... | 2004 | 14685857 |
| nectar production and transportation in the nectaries of the female cucumis sativus l. flower during anthesis. | in an effort to gain a greater understanding of nectar production, we studied the dynamic mechanisms of starch accumulation and transformation and nectar transportation in the cucumis sativus l. female flower. starch, which is the main precursor of nectar, accumulates in the epidermis and underlying parenchyma, with the most active accumulation occurring in the parenchyma cells within 3 days prior to anthesis. thereafter, the starch was successively hydrolyzed and the hydrolyte was transported f ... | 2004 | 15726811 |
| phosphoenolpyruvate carboxykinase in cucumber plants is increased both by ammonium and by acidification, and is present in the phloem. | in cucumber ( cucumis sativus l.), phosphoenolpyruvate carboxykinase (pepck) was shown by activity measurements and immunoblots to be present in leaves, stems, roots, flowers, fruit and seed. however, immunolocalisation showed that it was present only in certain cell types. pepck was present in the companion cells of the adaxial phloem of minor veins, the adaxial and abaxial phloem of larger veins, the internal and external phloem of vascular bundles in petioles and stems, the phloem in roots an ... | 2004 | 14991407 |
| plant uptake and translocation of highly weathered, soil-bound technical chlordane residues: data from field and rhizotron studies. | it has been observed that plants are susceptible to uptake from soil and in planta transport of technical chlordane, in spite of its hydrophobicity and sequestration within the soil matrix due to weathering. field and rhizotron studies were conducted with cucurbitaceae planted in highly weathered, chlordane-contaminated soil to investigate details of soil-to-plant contaminant uptake. in the field-work, cucurbita pepo l. (zucchini) was grown in soil at four levels of chlordane contamination: clea ... | 2004 | 15559292 |
| molecular cloning, characterization, tissue-specific and phytohormone-induced expression of calcium-dependent protein kinase gene in cucumber (cucumis sativus l.). | a cucumber cdna designated cscpk5 and encoding a calcium-dependent protein kinase (cscdpk5) was isolated and characterized. an open reading frame of 1542 bp was detected that could encode a protein of 514 amino acid residues with a calculated molecular mass of 56.5kda. comparison of the deduced amino acid sequence of cscdpk5 with sequences of other cdpks revealed the highest similarity (85%) to atcdpk6. as described for other cdpks, cscdpk5 has a long variable domain preceding a catalytic domain ... | 2004 | 15499908 |
| molecular characterization and isolation of the f/f gene for femaleness in cucumber ( cucumis sativus l.). | the biological processes leading to sex expression in plants are of tremendous practical significance for fruit production of many agricultural and horticultural crops. sex-expression studies in cucumber showed that the different sex types are determined by three major genes: m/m, f/f and a/a. the m/m gene in the dominant condition suppresses stamina development and thus leads to female flowers. the f/f gene in the dominant condition shifts the monoecious sex pattern downwards and promotes femal ... | 2004 | 15490106 |
| herbivory differentially affects male and female reproductive traits of cucumis sativus. | herbivory is an important selection pressure in the life history of plants. most studies use seed or fruit production as an indication of plant fitness, but the impact of herbivory on male reproductive success is usually ignored. it is possible that plants compensate for resources lost to herbivory by shifting the allocation from seed production to pollen production and export, or vice versa. this study examined the impact of herbivory by helix aspersa on both male and female reproductive traits ... | 2004 | 15375734 |
| application of micellar electrokinetic capillary chromatography to the analysis of uncharged pesticides of environmental impact. | a test mixture of five pesticides and metabolites (naphthalene acetamide, carbaryl, 1-naphthol, thiabendazole, and carbendazime) has been investigated by capillary electrophoresis with an ultraviolet diode array detector. these compounds were separated in <10 min by micellar electrokinetic capillary chromatography (mekc). mekc was performed in 30 mm ammonium chloride/ammonia buffer (ph 9.0) containing 15 mm sodium dodecyl sulfate. the lowest detection limit was obtained for the insecticide carba ... | 2004 | 15366822 |
| [ultrastructure of root cup meristematic cells of cucumis sativus l. seedlings under inhibition of root growth by synthetic cyclopentene beta,beta'-triketone]. | a study was made of the ultrastructure of root cap meristematic cells in the seedlings of cucumis sativus l., whose growth was inhibited by the action of 2-acetyl-4-metylcyclopent-4-en-1,3-dione (amcd). it is shown that the treatment of seeds by this substance at a concentration of 100 microg/ml leads to the main root inhibition by 73% and, as compared to the control, causes the highest changes in mitochondria that do not reach normal dimensions and undergo degeneration. amcd caused significant ... | 2004 | 15341126 |
| combined transcript and metabolite analysis reveals genes involved in spider mite induced volatile formation in cucumber plants. | many plants have an indirect defense against herbivores by emitting volatiles that attract carnivorous enemies of the herbivores. in cucumber (cucumis sativus) the production of carnivore attractants can be induced by herbivory or jasmonic acid spraying. from the leaves of cucumber plants with and without spider mite infestation, two subtractive cdna libraries were made that were enriched in cdna fragments up- or down-regulated by spider mite infestation. a total of 713 randomly selected clones ... | 2004 | 15310834 |
| differences in the inheritance stability of kanamycin resistance between transgenic cucumbers (cucumis sativus l.) containing two constructs. | a phenotypic segregation of kanamycin resistance conferred by nosnptii was investigated in two groups of transgenic cucumber lines one containing the pr-2duida-nosnptii construct and the other with the thaumatinii-nosnptii construct to the third and fifth generation, respectively. expression of the nptii gene was more stable and predictable in the progeny of the pr-2d lines than that of thaumatin lines. in t2 and t3 generations representing seven pr-2d primary transformants, 78% of the hemizygou ... | 2004 | 15306721 |
| developmental analyses reveal early arrests of the spore-bearing parts of reproductive organs in unisexual flowers of cucumber (cucumis sativus l.). | to understand the regulatory mechanisms governing unisexual flower development in cucumber, we conducted a systematic morphogenetic analysis of male and female flower development, examined the dynamic changes in expression of the c-class floral organ identity gene cum1, and assessed the extent of dna damage in inappropriate carpels of male flowers. accordingly, based on the occurrence of distinct morphological events, we divided the floral development into 12 stages ranging from floral meristem ... | 2004 | 15290297 |
| ontogenic and ecological control of metamorphosis onset in a carapid fish, carapus homei: experimental evidence from vertebra and otolith comparisons. | in carapus homei, reef colonisation is associated with a penetration inside a sea cucumber followed by heavy transformations during which the length of the fish is reduced by 60%. by comparing vertebral axis to otolith ontogenetic changes, this study aimed (i) to specify the events linked to metamorphosis, and (ii) to establish to what extent these fish have the ability to delay it. different larvae of c. homei were caught when settling on the reef and kept in different experimental conditions f ... | 2004 | 15286941 |
| proteomics of curcurbit phloem exudate reveals a network of defence proteins. | many different proteins can be separated from the sap of mature sieve tubes of different plant species. to date, only a limited number of those have been identified and functionally characterised. due to sieve tubes inability of transcription and translation, the proteins are most probably synthesised in the intimately connected companion cells and transported into the sieve elements through plasmodesmata. the specific protein composition of phloem sap suggests an important role of these protein ... | 2004 | 15276438 |
| toxicity of benzene, toluene, ethylbenzene, and xylene (btex) mixtures to sorghum bicolor and cucumis sativus. | 2004 | 15266698 | |
| a systemic small rna signaling system in plants. | systemic translocation of rna exerts non-cell-autonomous control over plant development and defense. long-distance delivery of mrna has been proven, but transport of small interfering rna and microrna remains to be demonstrated. analyses performed on phloem sap collected from a range of plants identified populations of small rna species. the dynamic nature of this population was reflected in its response to growth conditions and viral infection. the authenticity of these phloem small rna molecul ... | 2004 | 15258266 |
| molecular cloning and characterization of a cdna encoding a kinase in cucumis sativus and its expression by abiotic stress treatments. | as a part of an integrated study of stress-related gene expression, a cdna clone coding for a protein kinase in the root of cucumis sativus was isolated and characterized with respect to its sequence and the expression patterns upon various abiotic stress treatments. the predicted polypeptide of 352 amino acid residues contains characteristic features of both the serine/threonine and tyrosine kinase families. in vitro kinase assay confirmed that the isolated protein kinase has autophosphorylatio ... | 2004 | 15245919 |
| efficacy of acidic electrolyzed water for microbial decontamination of cucumbers and strawberries. | an examination was made of the efficacy of acidic electrolyzed water (acew, 30 ppm free available chlorine), ozonated water (5 ppm ozone), and a sodium hypochlorite solution (naocl, 150 ppm free available chlorine) for use as potential sanitizers of cucumbers and strawberries. acew and naocl reduced the aerobic mesophiles naturally present on cucumbers within 10 min by 1.4 and 1.2 log cfu per cucumber, respectively. the reduction by ozonated water (0.7 log cfu per cucumber) was significantly les ... | 2004 | 15222559 |
| rapid accumulation of hydrogen peroxide in cucumber roots due to exposure to low temperature appears to mediate decreases in water transport. | water transport across root systems of young cucumber (cucumis sativus l.) seedlings was measured following exposure to low temperature (lt, 8-13 degrees c) for varying periods of time. in addition, the amount of water transported through the stems was evaluated using a heat-balance sap-flow gauge. following lt treatment, hydrogen peroxide was localized cytochemically in root tissue by the oxidation of cerium (iii) chloride. the effects of hydrogen peroxide on the hydraulic conductivity of singl ... | 2004 | 15208334 |
| photosystem ii inhibition by moderate light under low temperature in intact leaves of chilling-sensitive and -tolerant plants. | photosystem ii (psii) activity was examsined in leaves of chilling-sensitive cucumber (cucumis sativus l.), tomato (lycopersicum esculentum l.), and maize (zea mays l.), and in chilling-tolerant barley (hordeum vulgare l.) illuminated with moderate white light (300 micro mol m(-2) s(-1)) at 4 degrees c using chlorophyll a fluorescence measurements. psii activity was inhibited in leaves of all the four plants as suggested by the decline in f(v)/f(m), 1/f(o) - 1/f(m), and f(v)/f(o) values. the cha ... | 2004 | 15153200 |
| combined effect of copper, cadmium, and lead upon cucumis sativus growth and bioaccumulation. | cucumis sativus (cucumber) was tested to assess an ecotoxicity in soils contaminated by the heavy metals copper (cu), cadmium (cd), and lead (pb) separately and in combinations. the toxicity endpoint was plant growth, which was measured as shoot and root lengths after 5 day exposure. sum of toxic unit (tu) at 50% inhibition for the mixture (ec50mix) was calculated from the dose (tu-based)-response relationships by the trimmed spearman-karber method. binary metal combinations of cu+cd, cu+pb, and ... | 2004 | 15142768 |
| [carpel development in male flowers of cucumber (cucumis sativus. l)]. | carpel development in cucumber male flowers was studied by morphological, histochemical and isoenzyme electrophoretic analyses. the results showed that: (1) cell number of the carpel in male flowers increased continuously during the development of male flowers, and the carpel in male flowers was abundant in rna content; (2) the carpel in male flowers at latter developmental stage was differentiated, and a placenta-like structure was formed in the carpel of male flowers during anthesis, while the ... | 2004 | 15133901 |
| an amperometric inhibitor biosensor for the determination of reduced glutathione (gsh) without any derivatization in some plants. | in this study, an amperometric biosensor based on cucumber tissue homogenate was developed for the determination of glutathione. cucumber (cucumis sativus l.) tissue homogenate was used as the biological material. the cucumber tissue homogenate was cross-linked with gelatine using glutaraldehyde and fixed on a pretreated teflon membrane. the principle of the measurements was based on the determination of the decrease in the differentiation of oxygen level which had been caused by the inhibition ... | 2004 | 15128102 |
| nitric oxide mediates the indole acetic acid induction activation of a mitogen-activated protein kinase cascade involved in adventitious root development. | recently, it was demonstrated that nitric oxide (no) and cgmp are involved in the auxin response during the adventitious rooting process in cucumber (cucumis sativus; pagnussat et al., 2002, 2003). however, not much is known about the complex molecular network operating during the cell proliferation and morphogenesis triggered by auxins and no in that process. anatomical studies showed that formation of adventitious root primordia was clearly detected in indole acetic acid (iaa)- and no-treated ... | 2004 | 15122018 |
| a role for brassinosteroids in the regulation of photosynthesis in cucumis sativus. | the effects of 24-epibrassinolide (ebr) spray application on gas-exchange, chlorophyll fluorescence characteristics, rubisco activity, and carbohydrate metabolism were investigated in cucumber (cucumis sativus l. cv. jinchun no. 3) plants grown in a greenhouse. ebr significantly increased the light-saturated net co(2) assimilation rate (a(sat)) from 3 h to 7d after spraying, with 0.1 mg l(-1) ebr proving most effective. increased a(sat) in ebr-treated leaves was accompanied by increases in the m ... | 2004 | 15107450 |
| uptake of perchlorate in terrestrial plants. | cucumber (cucumis sativus l.), lettuce (lactuca sativa l.), and soybean (glycine max) were used to determine uptake of the perchlorate anion (100 ppb) from sand. plants were watered with different ratios of hydrosol (a diluted solution of peters all-purpose plant food) to milli-q water (18 momega) to determine if the presence of other nutrients (such as nitrate) influenced perchlorate uptake. perchlorate concentrations in sand and plant tissues were determined weekly. perchlorate uptake was obse ... | 2004 | 15087162 |
| modulation by cytosolic components of proton pump activities in plasma membrane and tonoplast from cucumis sativus roots during salt stress. | the effect of nacl on the plasma membrane and tonoplast atpases measured as the hydrolytic and h(+)-pumping activity was studied. treatment of cucumber seedlings with salt increased the membrane-bound atpases of the plasma membrane as well as the tonoplast. in both types of membranes the stimulation of atp-hydrolysis was much higher than the stimulation of h(+)-transport suggesting that the salt- treatment of plants partially uncoupled the membrane proton pumps. it was shown that the soluble fra ... | 2004 | 15086821 |
| interactive effects of genotype, environment, and ontogeny on resistance of cucumber (cucumis sativus) to the generalist herbivore, spodoptera exigua. | host-plant genotype, environment, and ontogeny all play a role in determining plant resistance to herbivory, yet little is known about the nature of the interactions among these factors. we investigated resistance of cucumber plants cucumis sativus to the generalist herbivore spodoptera exigua in a manipulative experiment involving three factors. in particular, we tested the effects of bitter (cucurbitacins present) vs. sweet (cucurbitacins absent) plants (genotype), with or without previous her ... | 2004 | 15074656 |
| transient exposure to ethylene stimulates cell division and alters the fate and polarity of hypocotyl epidermal cells. | after transient exposure to the gaseous hormone ethylene, dark-grown cucumber (cucumis sativus) hypocotyls developed unusual features. upon ethylene's removal, the developing epidermis showed significant increases in cell division rates, producing an abundance of guard cells and trichomes. these responses to ethylene depended on the stage of development at the time of ethylene exposure. in the upper region of the hypocotyl, where cells were least differentiated at the onset of ethylene treatment ... | 2004 | 15047904 |
| growth and morphological responses to different uv wavebands in cucumber (cucumis sativum) and other dicotyledonous seedlings. | we examined the influence of short-term exposure of different uv wavebands on the fine-scale kinetics of hypocotyl growth of dim red light-grown cucumbers (cucumis sativus l.) and other selected dicotyledonous seedlings to evaluate: (1) whether responses induced by uv-b radiation (280-320 nm) are qualitatively different from those induced by uv-a (320-400 nm) radiation, and (2) whether different wavebands within the uv-b elicit different responses. responses to brief (30 min) irradiations with 3 ... | 2004 | 15032858 |
| exposure of roots of cucumber (cucumis sativus) to low temperature severely reduces root pressure, hydraulic conductivity and active transport of nutrients. | when root temperature dropped below 25 degrees c, there was a sharp drop in the root pressure (p(r)) and hydraulic conductivity of excised roots (lp(r)) of young cucumber (cucumis sativus l.) seedlings as measured with the root pressure probe. a detailed analysis of root hydraulics provided evidence for a larger reduction in the osmotic component of lp(r) (77%) in comparison with the hydrostatic component (34%) in response to the exposure of the root system to 13 degrees c. the activity of the p ... | 2004 | 15032838 |
| xyloglucan endotransglucosylase/hydrolase genes in cucumber (cucumis sativus) - differential expression during somatic embryogenesis. | defined changes in the cell wall directed by many proteins accompany every morphogenetic process in plants. xyloglucan endotransglucosylase/hydrolase proteins (xth; ec 2.4.1.207) have the potential to modify the hemicellulose matrix within the cell wall. cs-xth1 and cs-xth3 genes, which encode xth proteins, were found among numerous genes that are differentially expressed after the induction of cucumber somatic embryogenesis. the expression of these genes increased during somatic embryogenesis. ... | 2004 | 15032830 |
| phytoextraction of weathered p,p'-dde by zucchini (cucurbita pepo) and cucumber (cucumis sativus) under different cultivation conditions. | previous studies have shown that zucchini (cucurbita pepo) and cucumber (cucumis sativus) under field conditions are good and poor accumulators, respectively, of persistent organic pollutants from soil. here, each species was grown under three cultivation regimes: dense (five plants in 5 kg soil): nondense (one plant in 80 kg soil): and field conditions (two to three plants in approximately 789 kg soil). p,p'-dde and inorganic element content in roots, stems, leaves, and fruit were determined. i ... | 2004 | 15696707 |
| polymorphom of sexually different cucumber (cucumis sativus l.) nil lines. | isolations of polymorphic sequences of two pairs of the nil lines of cucumber (cucumis sativus l.), which differ with respect to sex, were carried out using the subtraction hybridization methods of dsc (differential subtraction chain) and gddsc (genetically directed dsc). 266 dsc tags were isolated from the entire genome region, and 38 gddsc tags were isolated from the region containing the sex genes. based on the obtained results, the methods used may be considered highly effective. the attaine ... | 2004 | 15647807 |
| transcriptional expression of a solanum sogarandium pgt::dhn10 gene fusion in cucumber, and its correlation with chilling tolerance in transgenic seedlings. | the expression pattern of a solanum sogarandinum pgt::dhn10 gene fusion encoding a dehydrin dhn10 protein and the potential role of that protein in cold tolerance in cucumber were analysed in three t1transgenic lines. an accumulation of dhn10 mrna was detected in the leaves, cotyledons, hypocotyls and roots of the transgenic seedlings both under the control conditions and after a cold treatment at 6 degrees c for 24 h. this was confirmed by rt-pcr. however, no dhn10 protein was detected by the a ... | 2004 | 15647805 |
| the cloning of sequences differentially transcribed during the induction of somatic embryogenesis in cucumber (cucumis sativus l.). | somatic embryogenesis in cucumber cell suspension culture is a convenient tool to study differential gene expression, particularly during the early stages of this process. in this study, we used the cucumber somatic embryogenesis system to detect genes that were differentially transcribed during the induction of embryo development. we identified and cloned 120 candidate cdna fragments from differential display gels. the selected cdnas were confirmed by reverse northern, and 83 were sequenced. th ... | 2004 | 15647797 |
| [differences in plant hormone level of cucumber seedlings grown under sulfur or xenon lamp]. | the primary mechanism of growth difference of cucumber (cucumis sativus l.) seedlings cultured under sulfur lamp and xenon lamp in a phytotron was investigated. compared with cucumber seedlings grown under xenon lamp, those under sulfur lamp were shorter, and the cell number in the middle hypocotyls epidermis and cortex of them were more (fig. 1, table 1). endogenous hormone analysis indicates that the content of iaa and ga(3) of seedlings under sulfur lamp were 17% and 24% lower, while aba cont ... | 2004 | 15643091 |
| [expression of cfl gene during differentiation of floral and vegetative buds in cucumber cotyledonary nodes cultured in vitro]. | cfl gene, a lfy homologue, was cloned from cucumber (cucumis sativus l.). in this paper, in situ hybridization was performed to analyze the expression pattern of cfl gene at the stage of floral and vegetative buds differentiation in cucumber cotyledonary nodes cultured in vitro. the results showed that at the stage of floral differentiation, cfl gene was strongly expressed in primordia, floral organ primordia, and each whirl of floral organs at the early stage of their formation, but weakly expr ... | 2004 | 15643084 |
| isolation and synthesis of shark-repelling saponins. | saponins are complex compounds that are composed of a saccharide attached to a steroid or triterpene. they are natural surfactants, or detergents. several important biological effects have been ascribed to saponins. they have been isolated from a great number of terrestrial plants. in the animal kingdom they are found in most sea cucumbers and starfish, whereas they are found only rarely in alcyonarians, gorgonians, sponges, and as shark-repelling compounds in fish. the present review deals with ... | 2004 | 15638249 |
| possible involvement of cs-acs1 and ethylene in auxin-induced peg formation of cucumber seedlings. | cucumber (cucumis sativus) seedlings develop a peg on the concave side of the gravitropically bending transition zone between the hypocotyl and the root after seed germination. peg initiation occurs in response to auxin when its levels in the concave side of the transition zone exceed a particular threshold through the graviresponse. ethylene also plays an important role in peg formation, but its relationship to auxin in this event is not understood. here, the role ethylene plays in auxin-induce ... | 2005 | 15585540 |
| a comparison of concentration ratios for technetium and nutrient uptake by three plant species. | technetium (tc) is a non-essential element for which accumulation mechanisms in plants have recently been discussed, but only from the viewpoint of existence of anion transport proteins in plant cells. in this study, using three kinds of plants (cucumis sativus l., raphanus sativus l., and brassica chinensis l.), uptake of tc and re (a chemical analogue of tc) were observed. the results showed that tc and re uptake occurred not only with water mass flow or active nutrient uptake, but also with u ... | 2005 | 15893799 |
| impact of twospotted spider mite (acari: tetranychidae) on growth and productivity of glasshouse cucumbers. | experiments were conducted to assess the damage of the glasshouse cucumber by twospotted spider mite, tetranychus urticae koch, and to investigate when the economic yield begins to decrease after t. urticae infestations. to assess the damage, dry matter partitioning in the cucumber plant was quantified and plant growth analyses were conducted at four different t. urticae infestation levels. t. urticae infestations decreased leaf productivity by reducing the total number of leaves per plant. appr ... | 2005 | 15889738 |
| exposure to pesticides and heavy work in greenhouses during pregnancy: does it effect birth weight? | work in greenhouses is performed in warm microclimate during the most time of the year, involves usually moderately intense or heavy work. the working conditions in greenhouses might involve also indirect exposure to pesticides resulting from contact with pesticide-treated flowers and vegetables. the aim of the study was to investigate whether the work in greenhouse during pregnancy adversely influenced infant birth weight and, if so, which of the two main potential hazards typical for such envi ... | 2005 | 15883817 |
| construction of two bac libraries from cucumber (cucumis sativus l.) and identification of clones linked to yield component quantitative trait loci. | two bacterial artificial chromosome (bac) libraries were constructed from an inbred line derived from a cultivar of cucumber (cucumis sativus l.). intact nuclei were isolated and embedded in agarose plugs, and high-molecular-weight dna was subsequently partially digested with bamhi or ecori. ligation of double size-selected dna fragments with the pecbac1 vector yielded two libraries containing 23,040 bamhi and 18,432 ecori clones. the average bamhi and ecori insert sizes were estimated to be 107 ... | 2005 | 15864523 |
| two plasma membrane h(+)-atpase genes are differentially expressed in iron-deficient cucumber plants. | aim of the present work was to investigate the involvement of plasma membrane (pm) h(+)-atpase (e.c. 3.6.3.6) isoforms of cucumber (cucumis sativus l.) in the response to fe deficiency. two pm h(+)-atpase cdnas (csha1 and csha2) were isolated from cucumber and their expression analysed as a function of fe nutritional status. semi-quantitative reverse transcriptase (rt)-pcr and quantitative real-time rt-pcr revealed in fe-deficient roots an enhanced accumulation of csha1 gene transcripts, which w ... | 2005 | 15854837 |
| holothurins b(2), b(3), and b(4), new triterpene glycosides from mediterranean sea cucumbers of the genus holothuria. | triterpene glycosides of three species of the mediterranean sea cucumbers holothuria polii, holothuria tubulosa, and holothuria sp. were studied. three new monosulfated biosides, holothurins b(2) (1), b(3) (2), and b(4) (3), along with the previously known holothurins a (4) and b (5) were isolated from the sea cucumber h. polii. triterpene glycosides belonging to holothurin a and b groups were found in h. tubulosa, while only one individual glycoside, holothurin a (4), was isolated from holothur ... | 2005 | 15844949 |
| influence of nutrient amendments on the phytoextraction of weathered 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene by cucurbits. | field experiments were conducted to determine the impact of nutrient amendments on the phytoextraction of weathered 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (p,p '-dde) by eight cultivars of cucurbits over a single growing season. four cultivars of cucurbita pepo ssp pepo are accumulators and extract percent level quantities of persistent organic pollutants (pops), whereas c. pepo ssp ovifera and cucumis sativus are nonaccumulators. the nonamended accumulators phytoextracted 1.0% of the p,p' ... | 2005 | 15839575 |
| gating of aquaporins by low temperature in roots of chilling-sensitive cucumber and chilling-tolerant figleaf gourd. | effects of low temperature (8 degrees c) on the hydraulic conductivity of young roots of a chilling-sensitive (cucumber, cucumis sativus l.) and a chilling-resistant (figleaf gourd, cucurbita ficifolia bouche) crop have been measured at the levels of whole root systems (root hydraulic conductivity, lp(r)) and of individual cortical cells (cell hydraulic conductivity, lp). exposure of roots to low temperature (lrt) for up to 6 d caused a stronger suberization of the endodermis in cucumber compare ... | 2005 | 15734792 |
| relationships between phenolic acid concentrations, transpiration, water utilization, leaf area expansion, and uptake of phenolic acids: nutrient culture studies. | phenolic acid treatments of cucumber seedlings (cucumis sativus cv "early green cluster") inhibited transpiration, water utilization, leaf area, and absolute and relative rates of leaf expansion. the cinnamic acids, ferulic and p-coumaric acids, were two to five times more inhibitory than the benzoic acids, p-hydroxybenzoic acid and vanillic acid. when phenolic acid concentrations were maintained at inhibitory concentrations through multiple successive treatments, percent inhibition of water uti ... | 2005 | 16222815 |
| accumulation of organotin compounds in the deep-sea environment of nankai trough, japan. | the concentration of butyltin (bt) and phenyltin (pt) compounds was measured in sediment, gastropods (colliloconcha nankaiensis), sea cucumbers (psychropotes verrucosa), galatheid crabs (munidopsis albatrossae and munidopsis subsquamosa), and bivalves (clyptogena tsubasa and clyptogena nautilei) collected from the nankai trough (water depth about 3000 m). sediment at depths of up to 1 cm was taken by a core sampler and at depths of up to 15 cm was taken by rake. the concentration of bts in sedim ... | 2005 | 16205985 |
| isolation of a partial sequence of a putative nucleotide sugar epimerase, which may involve in stamen development in cucumber (cucumis sativus l.). | sex determination is the most widely studied subject in cucumber. the sex of cucumber plants can be monoecious, hermaphrodite, gynoecious, androecious, or andromonoecious. besides environmental factors, three major genes, f/f, m/m, and a/a mainly govern the sex types in cucumber. regardless of their sex all floral buds are bisexual at the early bud stage. a stage specific arrest of either stamen or carpel leads to unisexual flower development. the possible downstream product of the interaction o ... | 2005 | 16133308 |
| a high-resolution karyotype of cucumber (cucumis sativus l. 'winter long') revealed by c-banding, pachytene analysis, and rapd-aided fluorescence in situ hybridization. | using molecular cytogenetic dna markers, c-banding, pachytene analysis, and fluorescence in situ hybridization (fish), a high-resolution karyotype was established in the cucumber. c-banding showed distinct hetero chromatic bands on the pericentromeric, telomeric, and intercalary regions of the chromosomes. the c-banding patterns were also consistent with the morphology of 4'-6-diamino-2-phenylindole dihydrochloride (dapi)-stained pachytene chromosomes. two repetitive dna fragments, csrp1 and csr ... | 2005 | 16121249 |
| [identification of expanding-related genes from young fruit of cucumber after pollination]. | differential expression of genes in young fruit of cucumber (cucumis sativus l.) before/after pollination was investigated via cdna amplified fragment length polymorphism (cdna-aflp). there were 64 transcript-derived fragments (tdfs) displayed specially in young fruits after pollination of 6-72 h (table 1), five of which were verified to be mainly expressed in young fruits by reverse northern dot blotting (fig.2). sequence data showed one tdf was a new gene encoding expansin and was named as cs- ... | 2005 | 16121012 |
| diving behaviour and decompression sickness among galapagos underwater harvesters. | diving conditions, dive profiles, vascular bubbles, and symptoms of decompression sickness (dcs) in a group of galapagos commercial divers are described. they harvest sea cucumbers from small boats with surface supplied air (hookah). dive profiles for 12 divers were recorded using dive loggers, and bubble formation was measured in the pulmonary artery. dcs symptoms were assessed by interview. a total of 380 immersions were recorded over a nine day period. the divers did on average 6.3 immersions ... | 2005 | 16119309 |
| [effects of cinnamic acid on physiological characteristics of cucumis sativus seedling]. | with substrate culture, this paper studied the effects of different concentrations of cinnamic acid on the physiological characteristics of cucumis sativus seedling. the results showed that 25 micromol.l(-1) of cinnamic acid had an inhibition effect on carotenoids, but a promotion effect on chlorophyll a and b. 50 micromol.l(-1) of this compound could significantly inhibit the photosynthetic rate, transpiration rate and root activity (p < 0.05), and this effect was getting stronger when the conc ... | 2005 | 16110671 |