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induction and inhibition of novel sulfotransferase in a human intestinal bacterium, eubacterium sp. a-44.induction and inhibition of a novel sulfotransferase produced by eubacterium sp. a-44 isolated from human feces have been studied. production of the enzyme was induced by phenylsulfate esters, sulfate donor substrates, but not by phenols, sulfate acceptor substrates, or inorganic sulfate. p-nitrophenylsulfate (pns), a good donor substrate, stimulated enzyme production more than 10-fold. sulfotransferase production was strongly inhibited by phenylphosphate esters. enzyme activity was competitivel ...19912070456
barbaloin stimulates growth of eubacterium sp. strain bar, a barbaloin-metabolizing bacterium from human feces.eubacterium sp. strain bar, isolated from human feces, transformed barbaloin to aloe-emodin anthrone in a basal medium lacking carbohydrate. barbaloin remarkably stimulated the growth of strain bar in the basal medium, the stimulative extent of the growth depending on the amount of barbaloin added. the addition of d-glucose, d-galactose, maltose, cellobiose, sucrose or d-amygdalin to the basal medium containing barbaloin caused a decrease of the growth stimulated by barbaloin to the growth level ...19912070461
induction of arthritis in rats by soluble peptidoglycan-polysaccharide complexes produced by human intestinal flora.peptidoglycan-polysaccharide complexes were isolated from feces of a healthy subject and from ileostomy fluid from 5 patients. peptidoglycan-polysaccharide complexes were tested for arthritogenicity in a rat model, by subcutaneous injection in freund's incomplete adjuvant. complexes from the healthy subject did not induce arthritis, but those from ileostomy fluid of 1 of the patients induced severe, chronic joint inflammation. we concluded that peptidoglycan-polysaccharide complexes from intesti ...19911747146
the microflora of periodontal sites showing active destructive progression.20 adult periodontitis (ap) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. by lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or betwee ...19911752997
primary structures of ribosomal proteins from the archaebacterium halobacterium marismortui and the eubacterium bacillus stearothermophilus.approximately 40 ribosomal proteins from each halobacterium marismortui and bacillus stearothermophilus have been sequenced either by direct protein sequence analysis or by dna sequence analysis of the appropriate genes. the comparison of the amino acid sequences from the archaebacterium h marismortui with the available ribosomal proteins from the eubacterial and eukaryotic kingdoms revealed four different groups of proteins: 24 proteins are related to both eubacterial as well as eukaryotic prot ...19911764513
[bacteriological examination of infections in the field of oral surgery].etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ...19911791332
effect of enzymatic sulfation on biochemical and pharmacological properties of catecholamines and tyrosine-containing peptides.substrate specificity of a novel sulfotransferase produced by eubacterium a-44 isolated from human feces has been studied. phenolic drugs, catecholamines, were good acceptors of this bacterial enzyme. with regard to dopamine, sulfation mostly occurred at the 4-aromatic hydroxy group. we also investigated the effects of enzymatic sulfation on pharmacologically active phenolic compounds. sulfation of phenolic compounds generally led to inactivation (e.g. tyramine and leu-enkephalin), with the exce ...19911799945
characterization of unusual tetracycline-resistant gram-positive bacteria.tetracycline-resistant tet m-negative isolates of actinomyces viscosus, eubacterium lentum, mobiluncus curtisii, and mobiluncus mulieris were screened with the tet k, tet l, and tet o dna probes. ten (71%) of the resistant mobiluncus strains hybridized with the tet o probe, two of the three e. lentum strains hybridized with the tet k probe, and the a. viscosus isolate hybridized with the tet l probe.19911810204
creatinine deiminase (ec 3.5.4.21) from bacterium bn11: purification, properties and applicability in a serum/urine creatinine assay.creatinine deiminase (ec 3.5.4.21) from the anaerobic microorganism bn11 has been purified to homogeneity by ammonium sulfate fractionation, gel filtration on sephacryl-s-300 superfine and chromatography on deae-sepharose c1 6b. the final enzyme preparation had a specific activity of 78 units per mg protein. analysis of creatinine deiminase by polyacrylamide gradient gel electrophoresis and fast-flow-liquid-chromatography gave a relative molecular mass of 285 kda and 288 kda, respectively. by tr ...19911819465
predominant cultivable flora in pericoronitis.the predominant cultivable flora in pericoronitis was investigated by culturing pus from affected sites in 20 patients. twenty colonies were picked at random from non-selective plates and identified using conventional biochemical and physiological tests, analysis of metabolic end-products by gas chromatography and protein profile analysis. the most frequently isolated organisms were prevotella (bacteroides) intermedia, peptostreptococcus micros, veillonella species, fusobacterium nucleatum and s ...19911820571
rapid method for altering bacterial ribosome-binding sequences for overexpression of proteins in escherichia coli.in an escherichia coli expression system, two genes, one from an anaerobic intestinal bacterium and one from e. coli, were overexpressed following the alteration of ribosome-binding (shine-dalgarno) sequences. for both genes, the polymerase chain reaction (pcr) was used to modify the ribosome-binding sequence and, at the same time, provide restriction endonuclease sequences at each end of the gene. these restriction endonuclease sequences were used for inserting the dna into the e. coli plasmid ...19911821779
the delta subunit of the chloroplast atpase is plastid-encoded in the diatom odontella sinensis.a 5.2 kb psti restriction fragment containing the atpa gene cluster of the plastic genome of the centric diatom odontella sinensis was cloned. sequencing revealed a reading frame of 561 bp separating the genes atpf and atpa, which is preceded by a putative ribosome binding site. the third nucleotide of the codon for the last amino acid of atpf is the first nucleotide of the initiation codon of the 561 bp reading frame. the amino acid sequence deduced from the nucleotide sequence of this gene (at ...19911826484
production of trypsin and chymotrypsin inhibitors by oral bacterial isolates.using an agar-skim milk gel method, over 300 oral bacterial isolates were tested for their ability to produce trypsin or chymotrypsin inhibitor. eleven bacterial strains, including seven isolates identified as bacteroides intermedius, were found to inhibit both proteolytic activities. the inhibitory factors of the 11 bacterial strains could be divided into two categories: i) heat-stable and dialysable, and ii) heat-labile and non-dialysable. the protease inhibitor activity of b. intermedius stra ...19911832450
bacteriological study of juvenile periodontitis in china.the predominant cultivable bacteria associated with juvenile periodontitis (jp) in china were studied for the first time. subgingival plaque samples were taken on paper points from 23 diseased sites in 15 jp patients and from 7 healthy sites in 7 control subjects. serially diluted plaque samples were plated on nonselective blood agar and on mgb agar, a selective medium for the isolation of actinobacillus actinomycetemcomitans. fifteen or more isolated colonies from each sample (in sequence witho ...19911832453
halobacterial s9 operon. three ribosomal protein genes are cotranscribed with genes encoding a trna(leu), the enolase, and a putative membrane protein in the archaebacterium haloarcula (halobacterium) marismortui.in the eubacterium escherichia coli the genes for the ribosomal proteins l13 and s9 form an operon consisting of two genes. the corresponding operon of the archaebacterium haloarcula marismortui (halobacterium marismortui was recently reassigned to the genus haloarcula [oren, a., ginzburg, m., ginzburg, b. z., hochstein, l. i., and volcani, b. e. (1990) int. j. syst. bacteriol. 40, 209-210] and is now called haloarcula marismortui) which is presented here, is much larger encoding three ribosomal ...19911840597
establishment of eubacterium cellulosolvens in the digestive tract of axenic and meroxenic mice: influence of feed cellulose content.the cellulolytic bacterial species present in the caecum and colon contents of conventionally reared mature mice did not become established in the digestive tract when inoculated to axenic mice, whatever the size of the inoculum or the cellulose content of the diet. the cellulolytic bacterium eubacterium cellulosolvens sc 10 isolated from mouse digestive flora was unable to become established in the digestive tract of axenic mice whatever the cellulose content of the diet; it requires a feed ric ...19911855647
cloning, sequencing, and expression of the gene coding for bile acid 7 alpha-hydroxysteroid dehydrogenase from eubacterium sp. strain vpi 12708.southern blot analysis indicated that the gene encoding the constitutive, nadp-linked bile acid 7 alpha-hydroxysteroid dehydrogenase of eubacterium sp. strain vpi 12708 was located on a 6.5-kb ecori fragment of the chromosomal dna. this fragment was cloned into bacteriophage lambda gt11, and a 2.9-kb piece of this insert was subcloned into puc19, yielding the recombinant plasmid pbh51. dna sequence analysis of the 7 alpha-hydroxysteroid dehydrogenase gene in pbh51 revealed a 798-bp open reading ...19911856160
nosocomial infections of ocular conjunctiva in newborns delivered by cesarian section.colonization of the ocular conjunctiva in newborns delivered by cesarian section occurs usually within the first day of life. we have studied the flora of the ocular conjunctiva at birth, from 19 newborns delivered by cesarian section, coming from two different maternity hospitals. ocular conjunctiva cultures yielded the main predominant flora in both maternity hospitals considered. the most common genus of this flora are: staphylococcus, corynebacterium and propionibacterium acnes. peptostrepto ...19911870845
thermostable cellobiohydrolase from the thermophilic eubacterium thermotoga sp. strain fjss3-b.1. purification and properties.exo-1,4-beta-cellobiohydrolase (ec 3.2.1.91) was isolated from the culture supernatant of thermotoga sp. strain fjss3-b.1, an extremely thermophilic eubacterium that grows optimally at 80 degrees c. the enzyme was purified to homogeneity as determined by sds/page and has an mr of 36,000. the enzyme is the most thermostable cellulase reported to date, with a half-life at 108 degrees c of 70 min in buffer. in a 40 min assay, maximal activity was recorded at 105 degrees c. cellobiohydrolase from st ...19911872819
[influence of cefodizime on intestinal bacterial flora].effects of cefodizime (cdzm), a new injectable cephem antibiotic, on the intestinal bacterial flora were studied in tetra-contaminated mice and in pediatric patients. cdzm was intramuscularly administered at a dose of 100 mg/kg once a day for 5 consecutive days to mice contaminated with 4 different species of organisms: escherichia coli, enterococcus faecalis, bacteroides fragilis and bifidobacterium breve. for 3 species except e. faecalis, bacterial populations in feces were markedly reduced af ...19911880920
dental bacterial plaques. nature and role in periodontal disease.antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ...19911890226
topographical and enzymatic characterization of amylases from the extremely thermophilic eubacterium thermotoga maritima.the hyperthermophilic eubacterium thermotoga maritima uses starch as a substrate, without releasing amylase activity into the culture medium. the enzyme is associated with the 'toga'. its expression level is too low to allow the isolation of the pure enzyme. using cycloheptaamylose and acarbose affinity chromatography and common chromatographic procedures, two enzyme fractions are obtained. they differ in specificity, ph-optimum, temperature dependence and stability. substrate specificity and ca ...19911709115
phylogeny of the whipple's-disease-associated bacterium.efforts to culture and identify the intracellular bacteria associated with whipple's disease have been unsuccessful. nucleotide sequencing and amplification by the polymerase chain reaction was done on the bacterial 16 s ribosomal dna present in a small-bowel biopsy specimen taken from a patient with whipple's disease. a search by computer for similar rrna sequences filed in databases showed the whipple's-associated organism to be most similar to bacteria of the rhodococcus, streptomyces, and ar ...19911714530
analysis of the gene encoding the rna subunit of ribonuclease p from t. thermophilus hb8.the gene for the rna subunit of ribonuclease p from the extreme thermophilic eubacterium t. thermophilus hb8 was cloned using oligonucleotide probes complementary to conserved regions of rnase p rna subunits from proteobacteria. the monocistronic gene and its flanking regions were sequenced. the gene is enclosed by a promoter and a rho-independent terminator. nuclease s1 protection analyses showed that the primary transcript is identical with the mature rna, i.e. no processing events are involve ...19911719485
posttranscriptional modification of trna in thermophilic archaea (archaebacteria).nucleoside modification has been studied in unfractionated trna from 11 thermophilic archaea (archaebacteria), including phylogenetically diverse representatives of thermophilic methanogens and sulfur-metabolizing hyperthermophiles which grow optimally in the temperature range of 56 (thermoplasma acidophilum) to 105 degrees c (pyrodictium occultum), and for comparison from the most thermophilic bacterium (eubacterium) known, thermotoga maritima (80 degrees c). nine nucleosides are found to be un ...19911708763
an extremely thermostable xylanase from the thermophilic eubacterium thermotoga.endo-1,4-beta-xylanase (ec 3.2.1.8) was isolated from the culture supernatant of thermotoga sp. strain fjss3-b.1, an extremely thermophilic anaerobic eubacterium which grows optimally at 80 degrees c. activity was purified 165-fold by anion-exchange and hydroxyapatite chromatography. the enzyme has an mr of 31,000 as determined by sds/page and 35,000 by analytical gel filtration. the optima for activity and stability for purified xylanase were between ph 5.0 and 5.5. at ph 5.5, which is the opti ...19911650183
[strict anaerobic bacteria: comparative study of various beta-lactam antibiotics in combination with tazobactam or sulbactam].the minimal inhibitory concentrations of piperacillin (pip) or cefotaxime (ctx) alone or in combination with tazobactam (taz) were determined against 168 anaerobes. all the strains were inhibited by pip + taz, but certain strains resistant to ctx + taz were found among b. fragilis, eubacterium and peptostreptococcus. the second investigations included 30 strains of bacteroides fragilis. concentrations of 2, 4 and 8 mg/l of taz and sulbactam (sul) were combined with piperacillin or cefotaxime. th ...19911652729
dgtp triphosphohydrolase, a unique enzyme confined to members of the family enterobacteriaceae.the enzyme dgtp triphosphohydrolase (dgtpase; ec 3.1.5.1) was assayed in partially purified extracts of several genera of bacteria, and it was found to be strictly confined to members of the family enterobacteriaceae. whereas 11 of 12 enteric bacteria had comparable activity for this enzyme, 8 of 8 nonenteric bacteria, including species in the very closely related genera vibrio and aeromonas, did not assay positively for this enzyme. when challenged with escherichia coli anti-dgtpase antiserum, ...19911657865
degradation of cellulose and forage fiber fractions by ruminal cellulolytic bacteria alone and in coculture with phenolic monomer-degrading bacteria.we hypothesized that bacterial species capable of metabolizing phenolic monomers may act as catalysts for forage fiber breakdown by increasing microbial access to cell wall polysaccharides. ruminal cellulolytic bacteria alone and in combination with phenolic-degrading bacteria were examined for differences in their ability to degrade fiber fractions of alfalfa or bromegrass. electron micrographs of fibrobacter succinogenes s85 cultured in combination with the ruminal phenolic-degrading organisms ...19911667013
identification and properties of an alpha-amylase from a strain of eubacterium sp. isolated from the rat intestinal tract.1. a bacterial amylase was isolated from the intestinal content of monoxenic rats inoculated with eubacterium sp. b86. 2. affinity chromatography on cross-linked starch allowed its separation from rat endogenous amylases. 3. the bacterial enzyme was characterized by its pi, molecular weight and action pattern. it behaves as a typical endo-amylase (alpha-amylase).19911673900
[bacteriological study on juvenile periodontitis].the predominant cultivable microflora of 23 pockets in 15 juvenile periodontitis (jp) patients was studied for the first time in china using the current anaerobic methodology. samples were taken with sterile paper points and dispersed on a vortex mixer. then the diluted samples were plated on the non-selective blood agar plates and selective mgb medium which favors the growth of actinobacillus actimycetemcomitans (aa) and incubated in anaerobic chamber for 5 days. from each sample 15 or more iso ...19911676335
percutaneous drainage of lung abscess in a malnourished child. 19911676504
fulminant eubacterium plautii infection following dog bite in asplenic man. 19911677721
phylogenetic depth of thermotoga maritima inferred from analysis of the fus gene: amino acid sequence of elongation factor g and organization of the thermotoga str operon.the gene (fus) coding for elongation factor g (ef-g) of the extremely thermophilic eubacterium thermotoga maritima was identified and sequenced. the ef-g coding sequence (2046 bp) was found to lie in an operon-like structure between the ribosomal protein s7 gene (rpsg) and the elongation factor tu (ef-tu) gene (tuf). the rpsg, fus, and tuf genes follow each other immediately in that order, which corresponds to the order of the homologous genes in the str operon of escherichia coli. the derived a ...19911920450
cos degradation by selected co-utilizing bacteria. scientific note. 19911929384
purification and characterization of a monomeric isocitrate dehydrogenase with dual coenzyme specificity from the photosynthetic bacterium rhodomicrobium vannielii.an isocitrate dehydrogenase able to function with either nadp or nad as coenzyme was purified to homogeneity from cell-free extracts of the purple photosynthetic eubacterium rhodomicrobium vannielii using a rapid two-step procedure involving dye-ligand affinity chromatography. the enzyme was obtained in 60% yield with specific activities of 23 u.mg protein-1 (nadp-linked reaction) and 18.5 u.mg protein-1 (nad-linked reaction). the purified enzyme was monomeric and migrated with an approximate mr ...19911935983
use of 23na nuclear magnetic resonance spectroscopy to determine the true intracellular concentration of free sodium in a halophilic eubacterium.we present new data obtained by 23na nuclear magnetic resonance spectroscopy, which can distinguish free intracellular sodium from cell-bound sodium, showing that the intracellular concentration of na+ the halophilic eubacterium vibrio costicola is only 5 to 20% of that in the extracellular medium. previous methods could not distinguish free intracellular na+ from that bound to cell structures, and it was believed that in halophilic eubacteria the total monovalent cation concentration inside mat ...19911938904
reactivity of igg from explant cultures of periapical lesions with implicated microorganisms.the presence of igg in periapical inflammatory lesions suggests that immune responses participate in the disease process. the purpose of this investigation was to study the reactivity of igg from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. a dot-enzyme-linked immunosorbent assay (dot-eli ...19911940739
comparison of the structure of archaebacterial ribosomal proteins equivalent to proteins l11 and l1 from escherichia coli ribosomes.the sequences of two ribosomal proteins from two widely divergent species of archaebacteria, halobacterium cutirubrum and sulfolobus solfataricus, have been deduced from the structure of their respective genes. these two proteins were found to be equivalent to the l11 and l1 ribosomal proteins of the eubacterium escherichia coli. sequence comparison revealed that the archaebacterial l11e (equivalent to e. coli l11) proteins are longer than the eubacterial protein due to a c-terminal extension of ...19911946333
the extremely thermophilic eubacterium, thermotoga maritima, contains a novel iron-hydrogenase whose cellular activity is dependent upon tungsten.thermotoga maritima is the most thermophilic eubacterium currently known and grows up to 90 degrees c by a fermentative metabolism in which h2, co2, and organic acids are end products. it was shown that the production of h2 is catalyzed by a single hydrogenase located in the cytoplasm. the addition of tungsten to the growth medium was found to increase both the cellular concentration of the hydrogenase and its in vitro catalytic activity by up to 10-fold, but the purified enzyme did not contain ...19911649830
folding intermediates of hyperthermophilic d-glyceraldehyde-3-phosphate dehydrogenase from thermotoga maritima are trapped at low temperature.d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic eubacterium, thermotoga maritima, is extremely thermostable showing a thermal transition beyond 105 degrees c. at low temperature, 'cold denaturation' becomes detectable only in the presence of destabilizing agents. reconstitution after preceding denaturation depends on temperature. at 0 degree c, no significant recovery of activity is detectable, whereas between 30 and 100 degrees c reactivation reaches up to 85%. shifting th ...19911915883
evidence for the establishment of aphid-eubacterium endosymbiosis in an ancestor of four aphid families.aphids (superfamily aphidoidea) contain eubacterial endosymbionts localized within specialized cells (mycetocytes). the endosymbionts are essential for the survival of the aphid hosts. sequence analyses of the 16s rrnas from endosymbionts of 11 aphid species from seven tribes and four families have indicated that the endosymbionts are monophyletic. furthermore, phylogenetic relationships within the symbiont clade parallel the relationships of the corresponding aphid hosts. our findings suggest t ...19911917864
microbial aspects of failing osseointegrated dental implants in humans.the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ...19911906537
properties of a 4-ene-3-ketosteroid-5 alpha-reductase in cell extracts of the intestinal anaerobe, eubacterium sp. strain 144.when eubacterium sp. 144 was grown in the presence of progesterone, extracts of these cells contained a 4-ene-3-ketosteroid-5 alpha-reductase (5 alpha-reductase). no evidence for the presence of a 5 beta-steroid-reductase or a 5 alpha to 5 beta-steroid-isomerase was found. 5 alpha-reductase activity was dependent on reduced methyl viologen as the electron donor and this could be generated biologically by adding pyruvate or h2 to cell extracts or chemically by adding sodium dithionite. nadh or na ...19911911427
kinetic studies on a novel sulfotransferase from eubacterium a-44, a human intestinal bacterium.a novel sulfotransferase purified from a human intestinal bacterium stoichiometrically catalyzed the transfer of a sulfate group of phenylsulfate esters to phenolic compounds. vmax values of the enzyme reaction were measured with various concentrations of a sulfate donor substrate, p-nitrophenylsulfate, and of a sulfate acceptor substrate, tyramine. double reciprocal plots of the acceptor concentration and vmax showed a linear correlation. one of the reaction products, tyramine o-sulfate, compet ...19911901853
molecular cloning of genes encoding major two subunits of a eubacterial v-type atpase from thermus thermophilus.the atpab genes which encode the alpha and beta subunits of membrane atpase from a thermophilic eubacterium, thermus thermophilus hb8, were cloned. the deduced amino-acid sequences of the alpha subunit (583 amino acids) and the beta subunit (478 amino acids) are only moderately similar to the alpha beta subunits of the f0f1-atpases, while they are highly similar to the major two subunits of the v-type atpases, a family of atpases which have been so far found in eukaryotic endomembrane vacuolar v ...19911836357
enzymatic sulfation of polyphenols related to tannins by arylsulfotransferase.this report discusses a novel type of arylsulfotransferase (ast) which was derived from human intestinal bacterium sulfated polyphenolic compounds when p-nitrophenyl sulfate (pns) was taken as a donor substrate. (+)-catechin, (+/-)-catechin, (-)-epicatechin and (-)-epicatechin gallate were better substrates than tyramine. (-)-epigallocatechin and (-)-epigallocatechin gallate were slightly worse substrates than tyramine. although gallic acid was a bad substrate, alkyl gallate esters were better s ...19911806284
histology of joint inflammation induced in rats by cell wall fragments of the anaerobic intestinal bacterium eubacterium aerofaciens.to study the arthropathic properties of human intestinal bacteria, cell wall fragments (cwf) of the anaerobic bowel bacterium eubacterium aerofaciens were injected intraperitoneally (i.p.) in arthritis-susceptible lewis rats. rat paw joints were subsequently studied for histopathological changes. a persisting synovitis accompanied by marginal erosions of cartilage and bone and a marked periosteal apposition of new bone tissue were the main features of the polyarthritis induced. these results are ...19911784889
surface layers of eubacterium yurii subsp. yurii and their possible role in test-tube brush formation and iron acquisition.eubacterium yurii subsp. yurii is an anaerobic, gram-positive rod. on isolation e. yurii forms cellular arrangements resembling test-tube brushes (ttb). although ttb decreased in size and number on repeated laboratory subculture in enriched media, media poor in available iron enhanced formation of these. plasmids were not demonstrated, even after chloramphenicol enhancement. to characterise the nature and possible physiological roles of the structures of the ttb, they were examined by transmissi ...19911753391
reverse transcription and dna amplification by a thermus thermophilus dna polymerase.a recombinant dna polymerase derived from the thermophilic eubacterium thermus thermophilus (tth pol) was found to possess very efficient reverse transcriptase (rt) activity in the presence of mncl2. many of the problems typically associated with the high degree of secondary structure present in rna are minimized by using a thermostable dna polymerase for reverse transcription, and predominantly full-length products can be obtained. the cdna can also be amplified in the polymerase chain reaction ...19911714296
interaction of selenoprotein pa and the thioredoxin system, components of the nadph-dependent reduction of glycine in eubacterium acidaminophilum and clostridium litorale [corrected].purification of protein pa of the glycine reductase complex from eubacterium acidaminophilum and clostridium litorale [corrected] was monitored by a new spectrophotometric assay. the procedure depended on a specific two- to threefold stimulation of a dihydrolipoamide dehydrogenase activity that is elicited by the interaction of a thioredoxin reductase-like flavoprotein and thioredoxin from both organisms. protein pa isolated from e. acidaminophilum by 75se labeling and monitoring of the dithioer ...19911917832
membrane-bounded nucleoid in the eubacterium gemmatata obscuriglobus.the freshwater budding eubacterium gemmata obscuriglobus possesses a dna-containing nuclear region that is bounded by two nuclear membranes. the membrane-bounded nature of the nucleoid in this bacterium was shown by thin sectioning of chemically fixed cells, thin sectioning of freeze-substituted cells, and freeze-fracture/freeze-etch. the fibrillar nucleoid was surrounded by electron-dense granules that were in turn enveloped by two nuclear membranes separated by an electron-transparent space. i ...199111607213
the origin and early evolution of nucleic acid polymerases.the hypothesis that vestiges of the ancestral rna-dependent rna polymerase involved in the replication of rna genomes of archean cells are present in the eubacterial rna polymerase beta' subunit and its homologues is discussed. we show that in the dna-dependent rna polymerases from the three cellular lineages a very conserved sequence of eight amino acids also found in a small rna-binding site previously described for the e. coli polynucleotide phosphorylase and the s1 ribosomal protein is pres ...199211538140
x-ray crystal structures of the oxidized and reduced forms of the rubredoxin from the marine hyperthermophilic archaebacterium pyrococcus furiosus.the structures of the oxidized and reduced forms of the rubredoxin from the archaebacterium, pyrococcus furiosus, an organism that grows optimally at 100 degrees c, have been determined by x-ray crystallography to a resolution of 1.8 a. crystals of this rubredoxin grow in space group p2(1)2(1)2(1) with room temperature cell dimensions a = 34.6 a, b = 35.5 a, and c = 44.4 a. initial phases were determined by the method of molecular replacement using the oxidized form of the rubredoxin from the me ...19921303768
13c-nmr study of autotrophic co2 fixation pathways in the sulfur-reducing archaebacterium thermoproteus neutrophilus and in the phototrophic eubacterium chloroflexus aurantiacus.the unresolved autotrophic co2 fixation pathways in the sulfur-reducing archaebacterium thermoproteus neutrophilus and in the phototrophic eubacterium chloroflexus aurantiacus have been investigated. autotrophically growing cultures were labelled with [1,4-13c1]succinate, and the 13c pattern in cell constituents was determined by 1h- and 13c-nmr spectroscopy of purified amino acids and other cell constituents. in both organisms succinate contributed to less than 10% of cell carbon, the major par ...19921572376
influence of decontamination on induction of arthritis in lewis rats by cell wall fragments of eubacterium aerofaciens. arthropathic properties of indigenous anaerobic bacteria.although the cause (or causes) of rheumatoid arthritis is unknown, many workers have suggested that microorganisms play a part. the intestinal flora in particular has been related to the development of joint inflammation. it has been shown previously that cell wall fragments of several anaerobic gram positive intestinal bacteria of human origin are arthritogenic after a single intraperitoneal injection in lewis rats. the part played by indigenous microflora in this model has now been studied by ...19921586251
biosynthesis of vitamin b12 in anaerobic bacteria. experiments with eubacterium limosum on the incorporation of d-[1-13c]erythrose and [13c]formate into the 5,6-dimethylbenzimidazole moiety.experiments on the incorporation of erythrose and formate into the 5,6-dimethylbenzimidazole moiety of vitamin b12 are described. in one experiment, a 1:1 mixture of d-[1-13c]erythrose and d-[1-13c]threose was added to a eubacterium limosum fermentation. the vitamin b12 formed was methylated at n3 of its 5,6-dimethylbenzimidazole part and degraded to 1,5,6-trimethylbenzimidazole. the 13c-nmr spectrum of this compound exhibited a single prominent signal at 109.5 ppm due to 13c labeling in c7. thi ...19921531794
immunohistology of joint inflammation induced in rats by cell wall fragments of eubacterium aerofaciens.after a single intraperitoneal injection of cell wall fragments of eubacterium aerofaciens, a main resident from the human intestinal flora, an acute arthritis develops within 2 days which is followed by a chronic arthritis that lasts at least 90 days. in an earlier report the histological appearance of the joint inflammation during this period has been described. in this study we investigated in more detail the cell types that are involved in the development of arthritis by using cell-type-spec ...19921519039
[serine proteinase from the archaebacterium halobacterium mediterranei--an analog of eubacterium subtilisin].a homogeneous serine proteinase was isolated from cultural filtrates of the extreme halophilic bacteria halobacterium mediterranei 1538 using affinity chromatography on bacitracin-sepharose, ultrafiltration and gel filtration on sephadex g-75, with a 48% yield and 260-fold purification. the enzyme was completely inactivated by specific inhibitors of serine proteinases, pmsf and dfp, as well as by hg2+ and pcmb. the enzyme activity was strongly dependent of nacl concentration, the enzyme being in ...19921391225
stability and reconstitution of d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic eubacterium thermotoga maritima.the molecular basis of thermal stability of globular proteins is a highly significant yet unsolved problem. the most promising approach to its solution is the investigation of the structure-function relationship of homologous enzymes from mesophilic and thermophilic sources. in this context, d-glyceraldehyde-3-phosphate dehydrogenase has been the most extensively studied model system. in the present study, the most thermostable homolog isolated so far is described with special emphasis on the st ...19921366231
the rieske fes center from the gram-positive bacterium ps3 and its interaction with the menaquinone pool studied by epr.the rieske 2fe2s center from bacillus ps3, a gram-positive thermophilic eubacterium, has been studied by epr spectroscopy. its redox midpoint potential at ph 7.0 was determined to be +165 +/- 10 mv and was found to decrease with an apparent slope of -80 mv/ph unit above ph 7.9. the qo-site inhibitor stigmatellin induced spectral changes analogous to those reported for rieske centers from mitochondria and chloroplasts. the redox midpoint potential of the ps3 rieske cluster was not affected by sti ...19921321134
structure of the glycan chain from the surface layer glycoprotein of clostridium thermohydrosulfuricum l77-66.the thermophilic eubacterium clostridium thermohydrosulfuricum l77-66 is covered by a crystalline surface layer composed of identical glycoprotein subunits which are arranged in a hexagonal lattice with centre-to-centre spacings of approx. 14.3 nm. sodium dodecyl sulphate-polyacrylamide gel electrophoresis of cell wall preparations showed the presence of several broadened, carbohydrate-containing bands in a molecular mass range of 90 to 200 kda. a total carbohydrate content of approx. 14% was de ...19921320936
arthritis by autoreactive t cell lines obtained from rats after injection of intestinal bacterial cell wall fragments.t cell lines (b13, b19) were isolated from the lymph nodes of lewis rats 12 days after an arthritogenic injection of cell wall fragments of eubacterium aerofaciens (ecw), a major resident of the human intestinal flora. these cell wall fragments consist of peptidoglycan polysaccharide complexes (ppc). the cell lines that bear the helper phenotype were arthritogenic in knee or ankle joints upon intravenous injection into irradiated lewis recipients. b13 was, however, not arthritogenic in irradiate ...19921733514
arthritogenicity of eubacterium species in the adjuvant arthritis model. 19921404109
cleavage efficiencies of model substrates for ribonuclease p from escherichia coli and thermus thermophilus.we compared cleavage efficiencies of mono-molecular and bipartite model rnas as substrates for rnase p rnas (m1 rnas) and holoenzymes from e. coli and thermus thermophilus, an extreme thermophilic eubacterium. acceptor stem and t arm of pre-trna substrates are essential recognition elements for both enzymes. impairing coaxial stacking of acceptor and t stems and omitting the t loop led to reduced cleavage efficiencies. small model substrates were less efficiently cleaved by m1 rna and rnase p fr ...19921281315
resistance of anaerobic bacteria to antimicrobial agents in spain.as a consequence of antibiotic consumption, the entire microbial ecosystem attached to man is evolving towards resistance. in spain, penicillin resistance (mic > 0.5 mg/l) is found in about 10% of peptostreptococcus, clostridium perfringens and eubacterium, and in 50% of veillonella. cefoxitin resistance ( > 2 mg/l) is present in 10 to 20% of peptostreptococcus and clostridium, and in 50% of eubacterium; 21% of bacteroides (fragilis group) strains are resistant to 16 mg/l. a very low rate of imi ...19921295754
the relationship between clinical symptoms and anaerobic bacteria from infected root canals.the purpose of this study was to investigate the correlation between the composition of bacterial flora from infected root canals and clinical symptoms. the materials evaluated consisted of 28 teeth from 25 patients with apical periodontitis. eubacterium were found to be significantly related to acute or chronic clinical symptoms and peptococcus, peptostreptococcus, and porphyromonas gingivalis to subacute clinical symptoms. we suggested that peptococcus, peptostreptococcus, eubacterium, porphyr ...19921298793
isolation and cloning of omp alpha, a coiled-coil protein spanning the periplasmic space of the ancestral eubacterium thermotoga maritima.we have discovered a new oligomeric protein component associated with the outer membrane of the ancestral eubacterium thermotoga maritima. in electron micrographs, the protein, omp alpha, appears as a rod-shaped spacer that spans the periplasm, connecting the outer membrane to the inner cell body. purification, biochemical characterization and sequencing of omp alpha suggest that it is a homodimer composed of two subunits of 380 amino acids with a calculated m(r) of 43,000 and a pi of 4.54. the ...19921330536
the nature of the last universal ancestor and the root of the tree of life, still open questions.the nature of the last universal ancestor to all extent cellular organisms and the rooting of the universal tree of life are fundamental questions which can now be addressed by molecular evolutionists. several scenarios have been proposed during the last years, based on the phylogenies of ribosomal rna and of duplicated proteins, which suggest that the last universal ancestor was either an rna progenote or an hyperthermophilic prokaryote. we discuss these hypotheses in the light of new data on t ...19921337989
the glna gene of the extremely thermophilic eubacterium thermotoga maritima: cloning, primary structure, and expression in escherichia coli.the structural gene (glna) encoding the glutamine synthetase (gs) of the extremely thermophilic eubacterium thermotoga maritima has been cloned on a 6.0 kb hindiii dna fragment. sequencing of the region containing the glna gene (1444 bp) showed an orf encoding a polypeptide (439 residues) with an estimated mass of 50,088 da, which shared significant homology with the gsi sequences of other bacteria (escherichia coli, bacillus subtilis) and archaea (pyrococcus woesei, sulfolobus solfataricus). th ...19921348781
immunoglobulins in milk from cows immunized with oral strains of actinomyces, prevotella, porphyromonas, and fusobacterium.immunization of pregnant cows with bacteria leads to the presence of high concentrations of specific antibodies in colostrum and milk. a total of 14 cows was immunized with single strains of heat-killed oral bacteria or pools of strains of actinomyces, porphyromonas, prevotella, and fusobacterium. two cows were treated with adjuvant alone. the mean percentages of igg1, igg2, igm, and iga in all of the milks were 83.8, 3.8, 9.3, and 3.1, respectively. elisa and whole cell agglutination assays dem ...19921354674
sequence analysis of an aphid endosymbiont dna fragment containing rpob (beta-subunit of rna polymerase) and portions of rpll and rpoc.the aphid schizaphis graminum is dependent on an association with a prokaryotic endosymbiont (buchnera aphidicola). the nucleotide (nt) sequence of a 5040 base pair (bp) dna fragment of b. aphidicola, homologous to the rpll-rpob-rpoc portion of the escherichia coli beta operon, was determined. the dna coded for the terminal 35 amino acids of rpll (large ribosomal subunit protein l7/l12), the complete rpob (beta-subunit of rna polymerase), and the first 209 amino acids of rpoc (beta'-subunit of r ...19921369199
phylogenetic evidence for the transfer of eubacterium suis to the genus actinomyces as actinomyces suis comb. nov.the 16s rrna primary structures of eubacterium suis dsm 20639t (t = type strain) and bifidobacterium bifidum dsm 20456t were determined by sequencing in vitro amplified rdna. sequence comparisons indicated that b. bifidum is moderately related to representatives of the genera actinomyces and mobiluncus. the closest relative of e. suis is actinomyces pyogenes. e. suis and a. pyogenes are more closely related phylogenetically to one another than to the other actinomyces species that have been inve ...19921371060
isolation and identification of intestinal bacteria from japanese tree frog (hlya japonica) with the special reference to anaerobic bacteria.the bacteria in the large intestines of eight japanese tree frogs (hlya japonica) were enumerated by using an anaerobic culture system. the microorganisms at approximately 3.1 x 10(9) bacteria per g (wet weight) of intestinal contents were present in the intestine of all the frogs tested. no difference of the total bacteria in the frog intestine was observed between two different incubation-temperatures (room temperature and 37 degrees c). eleven genera and 16 species were isolated from the frog ...19921391180
bacteriology of periodontal disease.the microbial flora associated with the periodontal tissues in health and disease is extremely complex, and much research is being directed toward identifying those species that may be etiologic agents or that can be used as prognostic indicators. recent work has resulted in changes in the taxonomic position of several periodontal species and the recognition that several others, particularly species of eubacterium and peptostreptococcus, as well as a novel oral spirochete, may be important in di ...19921392003
bacterial invasion of non-exposed dental pulp.anaerobic procedures were adopted to demonstrate the early bacterial invasion of non-exposed dental pulps, and to isolate and identify the bacteria. of 19 freshly extracted teeth which originally exhibited deep dentinal lesions, clinical examination and electric pulp testing showed that nine of them had no pulpal exposure. thus the pulps of these teeth were covered by clinically sound dentine beneath the carious lesion. bacteria were found to have invaded the pulps of six of these nine teeth. th ...19921399049
antibodies in normal and diseased pulps reactive with microorganisms isolated from deep caries.immunoglobulin molecules in the supernatant fluids (sf) from pulpal explant cultures have been observed to react with microorganisms implicated in infections of root canals. in this study, the reactivity of immunoglobulin molecules in the sf from normal and irreversible pulpitis pulps to six strains of predominant microorganisms isolated from the immediate layer of carious lesions above the pulps used for explant cultures was investigated using an enzyme-linked immunosorbent assay. two atcc stra ...19921402561
immunochemical and structural characterization of the antigenic polysaccharide from eubacterium saburreum t18.an antigenic surface polysaccharide produced by eubacterium saburreum strain t18, isolated from human dental plaque, was purified from formamide extract of whole cells. methylation analysis, smith degradation, optical rotation data and nuclear magnetic resonance spectra demonstrated that the purified antigen was a homopolysaccharide composed of d-glycero-d-galacto-heptose (hep.) residues. the structure of the repeating unit in the polysaccharide was: -[----6)-[alpha-hep.furanosyl-(1----4)]-beta- ...19921408355
anaerobic microflora associated with the pars oesophagea of the pig.in healthy pigs aged one to 35 days significant populations of microorganisms (5.5 to 6.9 log10 viable count cm-2 of the tissue) were found, by strict anaerobic techniques, to adhere to the pars oesophagea. the genera of anaerobes which were isolated included actinomyces, bifidobacterium, clostridium, lactobacillus, peptostreptococcus, streptococcus and veillonella and facultative anaerobes included escherichia, klebsiella, staphylococcus, streptococcus and yeasts. the microbial population adher ...19921410807
the role of tungstate and/or molybdate in the formation of aldehyde oxidoreductase in clostridium thermoaceticum and other acetogens; immunological distances of such enzymes.besides clostridium thermoaceticum and c. formicoaceticum other resting acetogenic clostridia such as c. aceticum and c. thermoautotrophicum and to a lesser extent non-clostridial acetogens such as butyribacterium methylotrophicum and eubacterium limosum were able to reduce propionate to propanol at the expense of carbon monoxide or formate. methylviologen usually increased the reduction rate. ten microm molybdate in the growth medium decreased this capability for c. thermoaceticum but increased ...19921417415
the organization and expression of essential transcription translation component genes in the extremely thermophilic eubacterium thermotoga maritima.a 5789-nucleotide-long ecori fragment from the genome of thermotoga maritima, identified by cross-hybridization to l11, l1, l10, and l12 ribosomal protein gene sequences from escherichia coli, was cloned and sequenced. the fragment encodes five trnas (trna(met1), anticodon complementary to aug; trna(met2), aug; trna(thr), aca; trna(tyr), uac; trna(trp), ugg), the transcription termination-antitermination factor nusg, the four 50 s subunit ribosomal proteins l11, l1, l10, and l12, and the amino-t ...19921429627
interactive effects of salt concentration and temperature on growth and lipid composition in the moderately halophilic bacterium vibrio costicola.the interactive effects of nacl concentration and growth temperature on the growth and lipid composition of the moderately halophilic eubacterium vibrio costicola have been investigated. vibrio costicola was shown to be capable of growth over the temperature range 4-37 degrees c. maximum growth yields were obtained at 30 degrees c when the optimum nacl concentration was 1.0 m nacl. in contrast with some previous studies, at higher or lower growth temperatures both the optimum and lower limit of ...19921458373
analysis of genomic dna from chlamydia trachomatis for dam and dcm methylation.chlamydia trachomatis is a gram-negative eubacterium with a dimorphic developmental cycle and obligate intracellular growth in the eucaryotic host. the dam transmethylase of escherichia coli methylates at the n6 position of adenine in the sequence 5'-gatc-3' and the dcm transmethylase adds methyl groups to the c5 position of the internal cytosines in the sequences 5'-ccwgg-3'. in contrast to e. coli, c. trachomatis dna appears to have unmethylated dam sites and only low level dcm methylation.19921459404
isolation of cdc group m-5 and staphylococcus intermedius from infected dog bites. 19921474272
[effect of meropenem on fecal flora in children].meropenem (mepm, sm-7338), a novel parenteral carbapenem antibiotic, was examined for its effect on intestinal flora in children. seven children with infectious diseases (3 male and 4 female children of age's ranging from 4 months to 8 years and 9 months weighing from 7.3 to 23.0 kg) were treated with mepm at doses ranging 10.3 to 40.5 mg/kg 3 or 4 times a day for 6 to 12 days. before, during and after the treatment, identities and numbers of various bacteria contained in 1 g of feces were deter ...19921479688
the endodontic microflora revisited.the microbial flora of 35 dental root canals were examined, taking care to maintain the viability of obligate anaerobes which accounted for 45% of total isolations, while streptococcal species accounted for 24% of the total species isolated. individual root canals yielded a maximum of eight bacterial species. a total of 40 different species was isolated of which the most prevalent were the facultative anaerobe streptococcus sanguis and the obligate anaerobe, peptostreptococcus micros (both in 23 ...19921479923
susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase.the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ...19921481764
relationship between substrate activity and pka value of phenols on sulfotransferase from eubacterium a-44.the relationship between the kinetics of the enzyme activity and the structural features of phenolic donor and of acceptor substrates was investigated with a sulfotransferase from eubacterium a-44, a human intestinal bacterium. the enzyme catalyzed the transfer of the sulfate group from the sulfate esters of phenol having a lower pka to phenols having a higher pka. when the km values for acceptor substrates were measured at their optimal ph, a linear plot for log10km versus the pka with a slope ...19921482408
associations between microbial species in dental root canal infections.the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ...19921494447
coaggregation studies of the eubacterium species.eubacterium species are gram-positive anaerobic rods that are frequently isolated from subgingival plaque of periodontal pockets. five eubacterium species were tested for their ability to coaggregate with 33 oral bacterial strains. using visual and turbidimetric assays, coaggregation was observed among eubacterium brachy, eubacterium nodatum, eubacterium alactolyticum and eubacterium limosum strains only when tested with fusobacterium nucleatum strains; eubacterium saburreum displayed only weak ...19921494452
sequence of the tufa gene encoding elongation factor ef-tu from thermus aquaticus and overproduction of the protein in escherichia coli.the sequence of the tufa gene from the extreme thermophilic eubacterium thermus aquaticus ep 00276 was determined. the gc content in third positions of codons is 89.5%, with an unusual predominance of guanosine (60.7%). the derived protein sequence differs from tufa- and tufb-encoded sequences for elongation factor tu (ef-tu) of thermus thermophilus hb8, another member of the genus thermus, in 10 of the 405 amino acid residues. three exchanges are located in the additional loop of ten amino acid ...19921499561
colonisation with digoxin-reducing strains of eubacterium lentum and clostridium difficile infection in nursing home patients.stool specimens obtained from 77 residents of a nursing home were analysed to determine the relationship between colonisation with digoxin-reducing strains of eubacterium lentum and infection with clostridium difficile. patients were categorised according to previous antibiotic treatment, prescription of enteral feedings, and pattern of bowel habits. colonisation with digoxin-reducing e. lentum was less common in subjects infected with c. difficile, in those treated with antibiotics previously, ...19921500644
the role of intestinal bacteria in the transformation of sodium picosulfate.sodium picosulfate, a laxative, was biotransformed to 4,4'-dihydroxydiphenyl-(2 pyridyl)-methane by intestinal flora that produced a novel sulfotransferase (not sulfatase). the biotransformation was activated by adding phenolic compounds such as phenol, acetaminophen and flavonoids. the enzyme activity related to this biotransformation was the highest in the contents of the caecum region of the intestine. the enzyme activity was 3.0 mumole/hr/g wet feces in humans and 0.75 in rats (ph 8.0). the ...19921507649
[immunoelectron-microscopic determination of the localization of tryptophanyl-trna synthetase in eubacterial cells of escherichia coli and methanococcus halophilus archaebacteria].localization of tryptophanyl-trna-synthetase (trs) was studied in halophilic archaebacterium methanococcus halophilus and eubacterium e. coli. ultrathin sections of the cells, fixed with glutaraldehyde and embedded in "lowicryl k4m" at -35 degrees c, were treated with colloidal gold complexes containing monoclonal antibodies aml against trs. the latter bind specifically to trs isolated both from eucaryotes, archae- and eubacteria. according to the label distribution three zones in m. halophilus ...19921508173
immunological homology among azoreductases from clostridium and eubacterium strains isolated from human intestinal microflora.azoreductases from several anaerobic intestinal bacteria have been shown to reduce azo dyes to carcinogenic aromatic amines. to evaluate the structural similarities of azoreductases from four species of clostridium and one species of eubacterium, a polyclonal antibody against purified clostridium perfringens azoreductase was generated in rabbits. this antibody inhibited the azoreductase activity of all five bacteria tested. elisa showed different degrees of binding of the antibody to various spe ...19921512704
sulfation of phenolic antibiotics by sulfotransferase obtained from a human intestinal bacterium.novel sulfotransferase which was isolated from eubacterium a-44, a human intestinal bacterium, sulfated phenolic antibiotics, such as amoxicillin, ceaodroxil and cefoperazone. the km values of sulfotransferase for these antibiotics were 6.9, 4.3 and 22.2 mm, respectively. the vmax values were 8.3, 3.3 and 1.6 mumol/min/mg protein. the optimal ph of the enzyme was 9.0, a weakly alkaline region. the antibacterial activity of amoxicillin was not altered by enzymic sulfation of the phenolic hydroxyl ...19921525935
microbiological evaluation of periapical infections in egypt.this study identifies and correlates proportions of bacteria in periapically involved anterior teeth of 85 adult egyptian patients. affected sites were free from caries and periodontal disease but had a history of trauma. the mean number of component bacterial species per specimen was 3.1. anaerobic bacteria were the dominant flora present in specimen cultures, comprising 73% (190/259) of cultivable bacteria. the most frequently isolated organisms were eubacterium species (68%), black-pigmented ...19921528629
serum igg reactive with oral anaerobic microorganisms associated with infections of endodontic origin.numerous species of bacteria have been implicated with infections of endodontic origin. the purpose of this study was to compare the levels of serum igg antibodies reactive with a panel of 10 oral anaerobic microorganisms implicated in infections of endodontic origin. serum samples were collected from 4 patient groups that included healthy patients without endodontic or periodontal disease, patients with chronic adult periodontal disease, patients with endodontic disease and patients with combin ...19921528630
predominant obligate anaerobes in human periodontal pockets.this study was carried out to investigate the predominant anaerobic bacteria of periodontal pockets in patients with advanced periodontitis, who had no previous treatment other than supragingival scaling, no history of recent or chronic systemic illness, nor any intake of antibiotics within 6 weeks prior to bacteriological sampling. care was taken not to ignore tiny-colony-forming anaerobes, by means of a stereoscope and an anaerobic glove box system. out of 422 (100%) isolates, 380 (90%) were o ...19921531505
in vitro activities of three semisynthetic amide derivatives of teicoplanin, mdl 62208, mdl 62211, and mdl 62873.mdl 62208, mdl 62211, and mdl 62873 are three semisynthetic amide derivatives of teicoplanin (mdl 62208 is an amide of teicoplanin aglycone, mdl 62211 is an amide of the teicoplanin a2 complex, and mdl 62873 is the corresponding derivative of peak a2-2 of the complex). the three semisynthetic glycopeptides were evaluated for in vitro antibacterial activity in comparison with the parent drug (teicoplanin) and vancomycin. a variety of gram-positive bacteria of clinical origin, whose species were c ...19921534977
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