Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| isolation and genotypic characterization of trueperella (arcanobacterium) pyogenes recovered from active cranial abscess infections of male white-tailed deer (odocoileus virginianus). | trueperella (arcanobacterium) pyogenes is a causative agent of suppurative infections in domestic and wild animals. in some populations of captive or free-ranging white-tailed deer (odocoileus virginianus), cranial abscess disease is an important source of mortality in adult males. although the pathogenesis of this disease is poorly understood, t. pyogenes has been isolated from active infections with other opportunistic bacteria. in this study, bacteria associated with cranial abscess infection ... | 2015 | 25831577 |
| influence of pathogenic bacteria species present in the postpartum bovine uterus on proteome profiles. | in the first 2-3 weeks after parturition >90% of dairy cows will have some form of uterine infection. uterine contamination with pathogens, such as trueperella (formerly arcanobacterium) pyogenes increases the risk of developing more severe endometritis, which can reduce conception rates. in this study, we compared the uterine proteome of cows infected with trueperella pyogenes with that of uninfected cows, using 2d gel electrophoresis, and identified annexins a1 and a2 (anxa1 and anxa2), apolip ... | 2015 | 24331367 |
| a retrospective study on clinical findings of 7300 cases (2007-2014) of barren female dromedaries. | the objective of this study was to investigate the clinical findings in barren female dromedaries examined for different complaints. female camels were examined for repeat breeding with regular heat interval (rb-r, n = 5444), refused mating (rm, n = 1299), repeat breeding with long heat interval (rb-l, n = 489), difficulties or bleeding during mating (dm, n = 53), and for manifestation of male-like behavior (mb, n = 15). the genital tracts of all females were evaluated using transrectal palpatio ... | 2015 | 25935372 |
| diagnosis and medical and surgical management of chronic infectious fibrinous pleuritis in an okapi (okapia johnstoni). | a 10-yr-old female okapi (okapia johnstoni) at the san diego zoo safari park was evaluated for intermittent malaise, inappetence, occasional cough, abdominal splinting, and licking at both flanks. physical examination revealed tachypnea, tachycardia, and fluid sounds on thoracic auscultation. transthoracic ultrasound showed multiple uniform, anechoic filled structures in the right and left pleural space. surgical exploration of the thoracic cavity revealed bilateral, mature, fibrous, compartment ... | 2015 | 26056911 |
| infectious lameness among migratory sheep and goats in north-west india, with particular focus on anaerobes. | various bacterial species, particularly dichelobacter nodosus, fusobacterium necrophorum, trueperella pyogenes (previously arcanobacterium pyogenes) and treponema spp., have been implicated in infectious conditions manifesting in lameness in sheep and goat populations. the current study reports the causes of infectious lameness in the north-western himalayan region, particularly himachal pradesh (hp), where no such study has been conducted in the past. among a total surveyed population of 27,586 ... | 2015 | 27044157 |
| molecular characterisation of mycoplasma species isolated from the genital tract of dorper sheep in south africa. | biochemical and molecular analysis were conducted on 34 strains of mycoplasma species isolated between 2003 and 2009 from the genital tract of clinically healthy dorper sheep and sheep with ulcerative vulvitis and balanitis. earlier publications identified the causative agent as mycoplasma mycoides mycoides large colony (mmmlc) and arcanobacterium pyogenes. the aims of the study were to characterise mycoplasma species isolated from the genital tract of dorper sheep with polymerase chain reaction ... | 2015 | 26244581 |
| molecular characterisation of <i>mycoplasma</i> species isolated from the genital tract of dorper sheep in south africa. | biochemical and molecular analysis were conducted on 34 strains of mycoplasma species isolated between 2003 and 2009 from the genital tract of clinically healthy dorper sheep and sheep with ulcerative vulvitis and balanitis. earlier publications identified the causative agent as mycoplasma mycoides mycoides large colony (mmmlc) and arcanobacterium pyogenes. the aims of the study were to characterise mycoplasma species isolated from the genital tract of dorper sheep with polymerase chain reaction ... | 2015 | 28235293 |
| [noma/cancrum oris: a neglected disease]. | noma is an aggressive orofacial gangrenous pathology that damages hard and soft tissues of the mouth and the face. throughout the centuries it has been present around the globe, but nowadays it has practically disappeared from developed countries and mainly affects children from the most disadvantaged places, especially in africa. noma disease is a multifactorial process; malnutrition, debilitating diseases (bacterial or viral systemic diseases, hiv-associated immunosuppression, etc.) and intrao ... | 2015 | 26437752 |
| virulence factor genes detected in the complete genome sequence of corynebacterium uterequi dsm 45634, isolated from the uterus of a maiden mare. | the complete genome sequence of the type strain corynebacterium uterequi dsm 45634 from an equine urogenital tract specimen comprises 2,419,437 bp and 2,163 protein-coding genes. candidate virulence factors are homologs of dip0733, dip1281, and dip1621 from corynebacterium diphtheriae and of sialidase precursors from trueperella pyogenes and chlamydia trachomatis. | 2015 | 26227590 |
| diversity and health status specific fluctuations of intrauterine microbial communities in postpartum dairy cows. | for the interpretation of clinical findings of endometritis and the development of disease prevention and intervention strategies a better understanding of the dynamics and interactions within intrauterine bacterial communities in healthy and diseased cows is required. to gain deeper insights into fluctuations within the uterine microbiota, intrauterine samples were collected from 122 cows at the day of calving, days 3, 9, 15, 21 and 28 postpartum. a total of 2052 bacterial isolates were identif ... | 2015 | 25497238 |
| minimum inhibitory concentrations of selected antimicrobials against escherichia coli and trueperella pyogenes of bovine uterine origin. | minimum inhibitory concentrations (mic) of 9 antimicrobials for isolates of 2 common bovine intrauterine bacterial pathogens, escherichia coli (n=209) and trueperella pyogenes (n=35), were determined using broth microdilution methodology. the isolates were recovered from dairy cows from 7 herds postpartum using the cytobrush technique. the pathogens were initially identified using phenotypic techniques. additionally, pcr was used to confirm the identity of t. pyogenes isolates and to categorize ... | 2015 | 25935246 |
| associations between intrauterine bacterial infection, reproductive tract inflammation, and reproductive performance in pasture-based dairy cows. | reproductive tract bacterial infections, particularly those caused by escherichia coli and trueperella pyogenes, can have a negative impact on reproductive performance. it has been hypothesized that the presence of e coli early postpartum may increase the risk of isolation of t pyogenes later postpartum. the objective of the present study was to examine associations between intrauterine bacterial infections with e coli and t pyogenes and any bacterial growth (irrespective of bacterial species), ... | 2015 | 25801340 |
| reduction of lactobacillus in the milks of cows with subclinical mastitis. | clinical and subclinical bovine mastitis are the most frequent diseases encountered on dairy farms worldwide, which cause significant economic loss and veterinary cost. the mastitic disease status is associated with increases in both milk bacterial pathogens and somatic cell count (scc). although it is well established that the mastitic pathogens generally correlate with the milk scc, to our knowledge, the correlation between the probiotic genus, lactobacillus, and the mastitic causative bacteri ... | 2015 | 25711409 |
| the prevalence of subclinical endometritis and intrauterine infections in repeat breeder cows. | the objectives of this study were to assess the prevalence of subclinical endometritis and the presence of common uterine pathogens in repeat breeder cows. a total of 121 cows with three or more consecutive artificial inseminations without conception and no clinical signs of disease were defined as repeat breeder cows and were enrolled in this trial. intrauterine samples were collected with the cytobrush technique to determine the prevalence of subclinical endometritis and bacteriologic infectio ... | 2015 | 25670153 |
| revisiting bovine pyometra--new insights into the disease using a culture-independent deep sequencing approach. | the bacteria present in the uterus during pyometra have previously been studied using bacteriological culturing. these studies identified fusobacterium necrophorum and trueperella pyogenes as the major contributors to the pathogenesis of pyometra. however, an increasing number of culture-independent studies have demonstrated that the bacterial diversity in most environments is underestimated in culture-based studies. consequently, fastidious pyometra-associated pathogens may have been overlooked ... | 2015 | 25550285 |
| an outbreak of dermatophilosis and caseous lymphadenitis mixed infection in camels (camelus dromedaries) in jordan. | this study describes and reports, for the first time, an outbreak of dermatophilosis that occurred concurrently with caseous lymphadenitis involving two camel herds (camelus dromedaries) in north jordan. | 2016 | 27249526 |
| mechanisms linking bacterial infections of the bovine endometrium to disease and infertility. | bacterial infections of the endometrium after parturition commonly cause metritis and endometritis in dairy cattle, and these diseases are important because they compromise animal welfare and incur economic costs, as well as delaying or preventing conception. here we highlight that uterine infections cause infertility, discuss which bacteria cause uterine disease, and review the evidence for mechanisms of inflammation and tissue damage in the endometrium. bacteria cultured from the uterus of dis ... | 2016 | 26952747 |
| identification of fimbrial subunits in the genome of trueperella pyogenes and association between serum antibodies against fimbrial proteins and uterine conditions in dairy cows. | understanding the role of fimbrial subunits during bacterial adherence and the host's immunological response against anchorage proteins is critical for the development of strategies to prevent pathogens from thriving. the objectives of the present study were to locate fimbria-related proteins in the genome of trueperella pyogenes (cp007519), define their importance for bacterial adherence, and evaluate the association between serum antibodies against fimbrial subunits and uterine health in dairy ... | 2016 | 26947291 |
| dynamics of postpartum endometrial cytology and bacteriology and their relationship to fertility in dairy cows. | endometrial samples were obtained from 56 consecutively calving dairy cows examined for endometrial cytology and for aerobic and anaerobic bacterial growth. changes over time, correlations between different cell types and between cell and bacterial populations and with fertility measures were calculated. the proportion of neutrophils in cytologic preparations decreased with time postpartum. other cell types did not change significantly with time. the proportion of neutrophils early (day 0 and 7) ... | 2016 | 26944540 |
| evidence of no protection for a recurrent case of pathogen specific clinical mastitis from a previous case. | the objective of this study was to determine whether the occurrence of a previous case of pathogen-specific clinical mastitis (cm) protects holstein dairy cows against a recurrent case. pathogens studied were escherichia coli, staphylococcus aureus, staphylococcus spp., streptococcus spp., klebsiella spp., and trueperella pyogenes. a total of 40 864 lactations (17,265 primiparous and 23,599 multiparous) from 19 835 cows from 5 large, high milk producing new york state dairy herds were analysed. ... | 2016 | 26568557 |
| detection of genes encoding multidrug resistance and biofilm virulence factor in uterine pathogenic bacteria in postpartum dairy cows. | reckless use of antibiotics and/or development of biofilm are the rationale for the development of multidrug resistance (mdr) of pathogenic bacteria. the objective of the present study was to detect mdr genes in trueperella pyogenes and to detect biofilm virulence factor (vf) genes in escherichia coli isolated from the uterus of postpartum dairy cows. uterine secretions from different parity postpartum holstein cows (n = 40) were collected using cytobrush technique after a sterile procedure from ... | 2016 | 26534827 |
| prevalence of bovine mastitis pathogens in bulk tank milk in china. | the objectives of this study were to estimate the herd prevalence of major mastitis pathogens in bulk tank milk (btm) in china dairy herds, to determine the relationship between the presence of mastitis pathogens and bulk tank milk somatic cell counts (btscc), and to investigate the impact of different dairy cattle farming modes and region on bacterial species. btm samples collected from 894 dairy herds in china were examined for the presence of mastitis pathogens. the flinders technology associ ... | 2016 | 27187065 |
| histophilosis as a natural disease. | histophilus somni is responsible for sporadic disease worldwide in cattle and, to a lesser extent, in small ruminants, bighorn sheep (ovis canadensis), and north american bison (bison bison). the importance of h. somni diseases can be attributed to improved clinical and laboratory recognition, combined with the growth in intensive management practices for cattle. although outbreaks of bovine histophilosis can occur year-round, in northern and southern hemispheres, it is most frequent in late fal ... | 2016 | 26847357 |
| isolation of trueperella pyogenes in a case of thoracic and abdominal abscess in a galago (otolemur garnettii). | an adult male galago (otolemur garnettii) presented for fight wounds following pairing for breeding. treatment was symptomatic with recovery. following resolution, the animal re-presented and died, despite additional treatment. | 2016 | 27338233 |
| application of a loop-mediated isothermal amplification (lamp) assay for molecular identification of trueperella pyogenes isolated from various origins. | in the present study 28 trueperella pyogenes strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (lamp) assay based on gene cpn60 encoding chaperonin. no cross reaction could be observed with control strains representing four species of genus trueperella and seven species of closely related genus arcanobacterium. the present cpn60 lamp assay might allow a reliable and low cost identification of t. pyogenes also in la ... | 2016 | 27242007 |
| a chimeric protein composed of the binding domains of clostridium perfringens phospholipase c and trueperella pyogenes pyolysin induces partial immunoprotection in a mouse model. | trueperella pyogenes and clostridium perfringens are two kinds of conditional pathogens frequently associated with wound infections and succeeding lethal complications in various economic livestock. pyolysin (plo) and phospholipase c (plc) are the key virulence factors of these two pathogens, respectively. in our study, a chimeric protein called rpc-pd4, which is composed of the binding regions of plo and plc, was synthesized. the toxicity of rpc-pd4 was evaluated. results revealed that rpc-pd4 ... | 2016 | 27473983 |
| prevalence of bovine milk pathogens in azorean pastures: mobile versus fixed milking machines. | the aims of the present study were (1) to evaluate the influence of using mobile (n=47) or fixed (n=45) milking machines in azorean herds on the apparent prevalence of several milk pathogens in bulk tank milk (btm) and (2) to determine whether separated subclinical mastitic cows can serve, in real time, as predictors of milk pathogen prevalence for the remaining animals at the herd level. the use of a mobile or fixed milking machine influenced (p≤0.05) the prevalence of staphylococcus aureus (72 ... | 2016 | 27843558 |
| complete genome sequence of vb_ecom-ufv13, a new bacteriophage able to disrupt trueperella pyogenes biofilm. | vb_ecom-ufv13, a member of the t4virus genus, shows lytic activity against escherichia coli and effectiveness in controlling the biofilm formed by trueperella pyogenes, which qualifies it as a promising component of phage cocktails for mastitis and metritis control. | 2016 | 27932642 |
| use of rumination and activity monitoring for the identification of dairy cows with health disorders: part ii. mastitis. | the objectives of this study were to evaluate (1) the performance of an automated health-monitoring system (ahms) to identify cows with mastitis based on an alert system (health index score, his) that combines rumination time and physical activity; (2) the number of days between the first his alert and clinical diagnosis (cd) of mastitis by farm personnel; and (3) the daily rumination time, physical activity, and his patterns around cd. holstein cows (n=1,121; 451 nulliparous and 670 multiparous ... | 2016 | 27372584 |
| relationship between escherichia coli virulence factors and postpartum metritis in dairy cows. | the objectives of this study were to report the prevalence of escherichia coli and trueperella pyogenes in the uterus of postpartum dairy cows before the onset of postpartum metritis (ppm) and to quantify their association with subsequent occurrence of ppm, to quantify the association between the presence of genes encoding e. coli virulence factors (vf) and ppm, and to determine the accuracy of using early postpartum uterine bacteriology results (bacteria and vf) to identify cows at risk of ppm. ... | 2016 | 27016836 |
| a longitudinal cohort study of acute puerperal metritis cases in swedish dairy cows. | acute puerperal metritis affects cows during the early postpartum period and causes fever, fetid vaginal discharge and general depression. the disease is severe and treatment with antimicrobials is often required. this study followed 79 swedish dairy cows with acute puerperal metritis with registered treatment and outcome in terms of recovery. bacteria isolated from the uterus and their susceptibility to penicillin were studied. clinical cases were assigned by participating practitioners who exa ... | 2016 | 27832812 |
| relationships between antimicrobial resistance, distribution of virulence factor genes and the origin of trueperella pyogenes isolated from domestic animals and european bison (bison bonasus). | trueperella pyogenes is an opportunistic pathogen causing suppurative infections in livestock and wild animals. although this bacterium is known for a long time, our knowledge about its pathogenicity is still insufficient. in this study the relationships between antimicrobial resistance profiles, distribution of virulence factor genes and the origin of t. pyogenes isolates were investigated. isolates (n = 97) from various infections in domestic animals and european bison were studied. minimal in ... | 2016 | 27154538 |
| dna vaccination based on pyolysin co-immunized with il-1β enhances host antibacterial immunity against trueperella pyogenes infection. | trueperella pyogenes is a commensal and opportunistic pathogen normally causes mastitis, liver abscesses and pneumonia of economically important livestock. to date, no specific control measure was reported to prevent t. pyogenes infections. in this study, we first constructed a recombinant plasmid pvax1-plo based on the main virulent factor pyolysin gene as dna vaccine against t. pyogenes infection. subsequently, transient expression of pvax1-plo and pcdna3.1/v5-fil-1β were identified in human e ... | 2016 | 27091688 |
| isoleucine 61 is important for the hemolytic activity of pyolysin of trueperella pyogenes. | pyolysin (plo) is a hemolysin secreted by trueperella pyogenes (t. pyogenes) and is important for the pathogenicity of t. pyogenes. oligomerization of plo monomers is a critical step in the process of hemolysis. however, the mechanisms of intermolecular interaction of plo monomers are still not clearly illuminated. in this study, two monoclonal antibodies (mabs) against plo, named ap-1a3 and ap-4f12, respectively, were generated firstly, of which ap-1a3 showed no or undetectable hemolysis inhibi ... | 2016 | 26711048 |
| associations among trueperella pyogenes, endometritis diagnosis, and pregnancy outcomes in dairy cows. | the objective of this study was to evaluate three commonly used methods for endometritis diagnosis by comparing each one's association with the presence of intrauterine trueperella pyogenes (tp) and reproductive performance. lactating holstein cows (n = 452) were evaluated a single time at 35 ± 3 days postpartum to diagnose endometritis on the basis of three criteria: presence of purulent vaginal discharge (pvd) detected by a metricheck device, presence of purulent uterine lavage fluid (pul), pr ... | 2016 | 26483313 |
| erythema multiforme associated with trueperella pyogenes bacteremia. | 2016 | 27988940 | |
| complete genome sequence of trueperella pyogenes, isolated from infected farmland goats. | trueperella pyogenes is a significant pathogen of livestock, causing diverse diseases, such as mastitis, liver abscessation, and pneumonia. in this study, we have reported the genome sequence of trueperella pyogenes 2012cq-zsh. moreover, several genes coding for virulence factors were found, such as pyolysin (pyo), nanh, nanp, cbpa, fimc, and fime. | 2016 | 27979957 |
| corrigendum to "isoleucine 61 in plo is important for the hemolytic activity of plo of trueperella pyogenes" [vet. microbiol. 182 (2016) 196-201]. | 2016 | 27771053 | |
| correlation of blood metabolite concentrations and body condition scores with persistent postpartum uterine bacterial infection in dairy cows. | to analyze the relationship of blood metabolite concentrations and body condition score (bcs) with persistent bacterial uterine infection, specifically that caused by trueperella pyogenes and anaerobic bacteria, uterine bacteriological swabs (n = 128) were collected from 64 holstein cows at 5 (w5) and 7 (w7) weeks postpartum, and the percentage of neutrophils in the endometrium was evaluated. blood glucose, total cholesterol (t-cho), blood urea nitrogen (bun), non-esterified fatty acid (nefa), a ... | 2016 | 27349443 |
| properties and antimicrobial susceptibility of trueperella pyogenes isolated from bovine mastitis in china. | trueperella (t.) pyogenes is an opportunistic pathogen that causes suppurative diseases in domestic animals. in this work, the properties, pathogenesis and phenotypic diversity of t. pyogenes isolates from bovine mastitis were studied. both pyolysin (plo) and collagen-binding protein (cbp) virulence factor genes were detected by pcr in all t. pyogenes isolates (n = 50). using the tissue culture plate method, 90% of t. pyogenes isolates were able to form biofilms. the minimum inhibitory concentra ... | 2016 | 26919137 |
| clinical and echocardiographic findings in an 8 year old brown swiss cow with myocardial abscess. | intramyocardial abscesses are rare in cattle and may lead to unspecific clinical signs. this case report describes the clinical and echocardiographic findings in an 8 year old brown swiss cow presented with an intramural myocardial abscess. the main clinical findings were anorexia, drop in milk yield, fever, tachycardia, and hyperfibrinogenemia. neither heart murmurs nor cardiac arrhythmias were present on auscultation. transthoracic echocardiographic examination revealed a prominent intramural ... | 2016 | 26900053 |
| bovine vaginal strain kocuria kristinae and its characterization. | kocuria spp. are widely distributed in nature. they are gram-positive, coagulase-negative, coccoid bacteria belonging to the family micrococcaceae, suborder micrococcineae, order actinomycetales, class actinobacteria. in general, limited knowledge exists concerning the properties associated with the representants of the genus kocuria, kocuria kristinae as well. following our previous results, k. kristinae kk2014 biocenol(™) (ccm 8628) was isolated from vagina of a healthy cow. its taxonomical al ... | 2016 | 26494240 |
| tethered bilayer membranes as a complementary tool for functional and structural studies: the pyolysin case. | we demonstrate the use of tethered bilayer lipid membranes (tblms) as an experimental platform for functional and structural studies of membrane associated proteins by electrochemical techniques. the reconstitution of the cholesterol-dependent cytolysin (cdc) pyolysin (plo) from trueperella pyogenes into tblms was followed in real-time by electrochemical impedance spectroscopy (eis). changes of the eis parameters of the tblms upon exposure to plo solutions were consistent with the dielectric bar ... | 2016 | 27211243 |
| identification of escherichia coli and trueperella pyogenes isolated from the uterus of dairy cows using routine bacteriological testing and fourier transform infrared spectroscopy. | uterine disorders are common postpartum diseases in dairy cows. in practice, uterine treatment is often based on systemic or locally applied antimicrobials with no previous identification of pathogens. accurate on-farm diagnostics are not available, and routine testing is time-consuming and cost intensive. an accurate method that could simplify the identification of uterine pathogenic bacteria and improve pathogen-specific treatments could be an important advance to practitioners. the objective ... | 2016 | 27894322 |
| umbilical infections in foals: microbiological investigation and management. | this study aims to investigate the bacteria involved in equine omphalitis and their susceptibility to antimicrobial drugs, and consequently to provide guidelines concerning the most suitable treatment protocol in accordance with the clinical, ultrasound and laboratory findings. forty foals aged between one and 30 days were evaluated in the course of this investigation. an ultrasound examination of all umbilical remnants was carried out carefully in all foals; umbilical swabs were collected for b ... | 2017 | 28314783 |
| survival of streptococcus suis, streptococcus dysgalactiae and trueperella pyogenes in dry-cured iberian pork shoulders and loins. | dry-cured hams, shoulders and loins of iberian pigs are highly appreciated in national and international markets. salting, additive addition and dehydration are the main strategies to produce these ready-to-eat products. although the dry curing process is known to reduce the load of well-known food borne pathogens, studies evaluating the viability of other microorganisms in contaminated pork have not been performed. in this work, the efficacy of the dry curing process to eliminate three swine pa ... | 2017 | 27697171 |
| trueperella pyogenes isolated from dairy cows with endometritis in inner mongolia, china: tetracycline susceptibility and tetracycline-resistance gene distribution. | trueperella pyogenes plays a crucial role in endometritis pathogenesis and is also associated with many infections, including metritis, mastitis, arthritis and liver abscessation, in many domestic animals. in this study, we investigated the prevalence of tetracycline resistance in t. pyogenes isolated from dairy cows with endometritis in inner mongolia, china, and we assessed tetracycline-resistance gene distribution among the isolates. our results indicated that 68.7% and 62.5% of the isolates ... | 2017 | 28188901 |
| factors affecting the incidence and outcome of trueperella pyogenes mastitis in cows. | the main factors affecting the outcome of trueperella pyogenes (t. pyogenes) mastitis were examined through a survey of diagnostic data and interviews relating to the occurrence of t. pyogenes mastitis in 83 quarters from 82 holstein cows between august 2012 and april 2014. ultimately, one cow was sold during the examination, and 82 quarters from 81 cows were used for analysis on prognosis. t. pyogenes mastitis occurred year round in both lactating and dry cows. the incidence of t. pyogenes mast ... | 2017 | 28163273 |
| presence of bacteria in the endometrium and placentomes of pregnant cows. | bacterial invasion of the bovine uterus during the postpartum period occurs in most cows, but the general consensus is that these bacteria are eliminated before the next pregnancy. the pregnant uterus has therefore hitherto been considered a sterile environment, but this assumption has now been challenged by recent studies in humans, which indicate that bacteria can be present in the placenta of term pregnancies without causing abortion. the aim of the present study was therefore to investigate ... | 2017 | 28708498 |
| evaluation of a multiplex real-time pcr for detection of four bacterial agents commonly associated with bovine respiratory disease in bronchoalveolar lavage fluid. | pasteurella multocida, mannheimia haemolytica, histophilus somni and trueperella pyogenes are four bacterial agents commonly associated with bovine respiratory disease (brd). in this study a bacterial multiplex real-time pcr (the respocheck pcr) was evaluated for the detection in bronchoalveolar lavage fluid (balf) of these four bacterial agents. | 2017 | 28705198 |
| metritis vaccination in holstein dairy heifers using a herd-specific multivalent vaccine - effects on uterine health and fertility in first lactation. | in cattle, acute puerperal metritis (apm) compromises animal welfare and has an economic impact to the farmer because of the detrimental effects on reproductive performance, milk production, and survivability. the aim of this randomized clinical study was to investigate the effects of a prepartum immunization using a herd-specific multivalent vaccine on incidence of apm, prevalence of clinical endometritis (ce), and selected measures of reproductive performance in primiparous holstein cows. preg ... | 2017 | 28760665 |
| traumatic pericarditis caused by a bamboo twig in captive waterbuck (kobus ellipsiprymnus). | a 19-year-old captive male waterbuck (kobus ellipsiprymnus) exhibited traumatic pericarditis at necropsy. the animal weighed 182 kg at necropsy and revealed no remarkable findings in external observation. severe pericardial adhesions with fibrosis, hepato-diaphragmatic adhesions, straw-colored ascites and hepatosplenomegaly were observed upon examining the internal organs. perforations made by a 12-cm-long sharp-ended bamboo twig were detected in the reticulum, diaphragm, pericardium, lung and l ... | 2017 | 28757525 |
| the complete genome sequence of trueperella pyogenes ufv1 reveals a processing system involved in the quorum-sensing signal response. | we present here the complete genome sequence of trueperella pyogenes ufv1. the 2.3-mbp genome contains an extremely interesting ai-2 transporter and processing system related to the quorum-sensing signal response. this specific feature is described in this species for the first time and might be responsible for a new pathogenic behavior. | 2017 | 28729265 |
| bovine endometrial epithelial cells scale their pro-inflammatory response in vitro to pathogenic trueperella pyogenes isolated from the bovine uterus in a strain-specific manner. | among different bacteria colonizing the bovine uterus, trueperella pyogenes is found to be associated with clinical endometritis (ce). the ability of cows to defend against t. pyogenes infections depends on the virulence of invading bacteria and on the host's innate immunity. therefore, to gain insights into bacterial factors contributing to the interplay of this host pathogen, two strains of t. pyogenes were included in this study: one strain (tp2) was isolated from the uterus of a postpartum d ... | 2017 | 28680854 |
| virulence markers associated with trueperella pyogenes infections in livestock and companion animals. | trueperella pyogenes is an opportunistic pathogen that causes diverse pyogenic infections in livestock. the genes that encode the exotoxin pyolysin (plo) and other putative factors that promote adhesion of pathogen to host cells (fimbriae fima, fimc, fime, fimg, neuraminidases nanh, nanp, and collagen-binding protein cbpa) have been associated with virulence, particularly in mastitis and uterus infections of dairy cows. however, the role of these virulence markers in the pathogenicity of the age ... | 2017 | 28561264 |
| trueperella pyogenes isolated from a brain abscess of an adult roebuck (capreolus capreolus). | the present study was designed to characterize phenotypically and genotypically a trueperella pyogenes strain isolated from a brain abscess of an adult roebuck (capreolus capreolus). the species identity could be confirmed by phenotypical investigations, by maldi-tof ms analysis, and by sequencing the 16s ribosomal rna (rrna) gene, the 16s-23s rrna intergenic spacer region (isr); by sequencing the target genes rpob, gap, and tuf; and by detection of t. pyogenes chaperonin-encoding gene cpn60 wit ... | 2017 | 28534230 |
| characterization of porcine trueperella pyogenes by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms), molecular typing and antimicrobial susceptibility profiling in sao paulo state. | trueperella pyogenes can be found as a commensal or pathogenic bacterium among animals causing a variety of pyogenic infections in several species. the agent appears to act primarily as an opportunistic pathogen but may disseminate and produce metastatic abscesses accompanied or not by mastitis, metritis or pneumonia. in this study, 30 porcine t. pyogenes strains were identified by maldi-tof ms and 16s rrna sequencing and further evaluated in relation to their resistance and genetic profiles thr ... | 2017 | 28504095 |
| validity of somatic cell count as indicator of pathogen-specific intramammary infections. | the objective of this study was to determine whether somatic cell count (scc) was an effective test, with a sensitivity exceeding 85%, to determine species-specific bacterial infections. in addition, the relation between the scc and various udder pathogen groups was investigated. scc thresholds of greater than 200 000 cells/ml were used in quarter and greater than 150 000 cells/ml in composite milk samples. a retrospective study was conducted on a data set for 89 635 quarter and 345 467 composit ... | 2017 | 28470079 |
| a combined clostridium perfringens/trueperella pyogenes inactivated vaccine induces complete immunoprotection in a mouse model. | clostridium perfringens (c. perfringens) and trueperella pyogenes (t. pyogenes) are two bacterial pathogens frequently associated with wound infections and following lethal complications in livestock. however, prudent use of antimicrobial agents is highly required given the emergence of multidrug-resistant strains of both bacteria and need for food safety. in the current study, a combined vaccine, composed of inactivated c. perfringens and t. pyogenes, was prepared. the amount of formaldehyde be ... | 2017 | 28427828 |
| a review of the ongoing discussion about definition, diagnosis and pathomechanism of subclinical endometritis in dairy cows. | in the last decade, several new aspects of the inflammation of the bovine endometrium have been investigated and described, including a new definition of subclinical endometritis. this review summarizes the recent discussion about the definition, diagnosis and pathomechanism of subclinical endometritis. subclinical endometritis also referred to as cytological endometritis is defined by findings of endometrial cytology, which is usually performed with the cytobrush-technique or by low-volume flus ... | 2017 | 28407857 |
| fatal infection in three grey slender lorises (loris lydekkerianus nordicus) caused by clonally related trueperella pyogenes. | trueperella pyogenes is a worldwide known bacterium causing mastitis, abortion and various other pyogenic infections in domestic animals like ruminants and pigs. in this study we represent the first case report of three unusual fatal infections of grey slender lorises caused by trueperella pyogenes. meanwhile, this study represents the first in-depth description of the multilocus sequence analysis (mlsa) on t. pyogenes species. | 2017 | 28851356 |
| presence and localization of bacteria in the bovine endometrium postpartum using fluorescence in situ hybridization. | the aim of this study was to investigate bacterial invasiveness of the bovine endometrium during the postpartum period. fluorescence in situ hybridization was applied to endometrial biopsies using probes for fusobacterium necrophorum, porphyromonas levii, trueperella pyogenes, escherichia coli and a probe for bacteria in general (the overall domain bacteria) to determine their tissue localization. holstein cows were sampled at three time points postpartum (t1: 4-12 days postpartum, t2: 24-32 day ... | 2017 | 28237333 |
| presence of coxiella burnetii dna in inflamed bovine cardiac valves. | bacterial endocarditis is a recognised disease in humans and animals. in humans, infection with coxiella burnetii can cause endocarditis, but this has not been investigated thoroughly in animals. endocarditis in cattle is a common post-mortem finding in abattoirs and studies have identified trueperella pyogenes as a major cause. despite exposure of cattle to c. burnetii, the significance of this particular bacterium for development and progression of endocarditis has not been studied in detail. ... | 2017 | 28274243 |
| disease complexity in a declining alaskan muskox (ovibos moschatus) population. | the muskox ( ovibos moschatus ) population inhabiting the eastern north slope (ens) of alaska, us declined dramatically during 1999-2006, whereas populations in western alaska (wa) were stable or increasing. to understand morbidity and mortality factors contributing to the decline, alaska department of fish and game conducted pathologic investigations of carcasses from 2005 until 2008. additionally, archived sera from both ens and wa muskoxen collected during 1984-92, before the documented begin ... | 2017 | 28099077 |
| development of a one-run real-time pcr detection system for pathogens associated with bovine respiratory disease complex. | bovine respiratory disease complex (brdc) is frequently found in cattle worldwide. the etiology of brdc is complicated by infections with multiple pathogens, making identification of the causal pathogen difficult. here, we developed a detection system by applying taqman real-time pcr (dembo respiratory-pcr) to screen a broad range of microbes associated with brdc in a single run. we selected 16 bovine respiratory pathogens (bovine viral diarrhea virus, bovine coronavirus, bovine parainfluenza vi ... | 2017 | 28070089 |
| uterine infection with bovine herpesvirus type 4 in dairy cows. | diseases of the reproductive tract are a frequent problem in dairy herds. herpesviruses are uterine pathogens also involved in other clinical diseases; for example, bovine herpesvirus type 4 bohv-4 induces abortion, enteritis, metritis, pneumonia and vaginitis, but it can also be detected in healthy cows. the role of bohv-4 in the development of clinical endometritis (ce) or subclinical endometritis (se) has not clearly been described. therefore, the objective of this study was to describe the p ... | 2017 | 27859701 |
| prospective case-control study of toe tip necrosis syndrome (ttns) in western canadian feedlot cattle. | a case-control study was conducted to investigate potential risk factors for toe tip necrosis syndrome (ttns) in western canadian feedlot cattle. feedlot veterinarians provided hooves from 222 animals that died of either ttns ("cases") or from all other causes ("controls"). the claws were sectioned by researchers to confirm the diagnoses; there was very good agreement between the practitioners' field diagnosis and that of the researchers (cohen's kappa = 0.81; p < 0.001). the sole thickness of t ... | 2017 | 28246411 |
| comparative transcriptome analysis of trueperella pyogenes reveals a novel antimicrobial strategy. | trueperella pyogenes is a prevalent opportunistic bacterium that normally causes diverse suppurative lesions, endometritis and pneumonia in various economically important animals. although the genomic information of this species has been announced, little is known about its functional profiles. in this study, by performing a comparative transcriptome analysis between the highly and moderately virulent t. pyogenes isolates, we found the expression of a luxr-type dna-binding response regulator, pl ... | 2017 | 28144921 |
| bacterial invasion of the uterus and oviducts in bovine pyometra. | pyometra is a common disease of cattle that causes infertility and thereby financial losses to the cattle industry. bacteria involved in the development and progression of pyometra have been investigated by microbial culture but their tissue invading abilities, which is an important aspect of bacterial pathogenicity and development of lesions, have not been investigated. bacterial invasion of the uterus and oviducts was studied in 21 cows diagnosed with pyometra at the time of slaughter by apply ... | 2017 | 28257873 |