Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| lactate dehydrogenase from streptococcus mutans: purification, characterization, and crossed antigenicity with lactate dehydrogenases from lactobacillus casei, actinomyces viscosus, and streptococcus sanguis. | a cytoplasmic fructose-1,6-diphosphate-dependent lactate dehydrogenase (ldh; ec 1.1.1.27) from streptococcus mutans omz175 was purified to homogeneity as judged by sodium dodecyl sulfate-gel electrophoresis. the purification consisted of ammonium sulfate precipitation of the cytoplasmic fraction, deae-sephacel and blue-sepharose cl.6b chromatography, and sephacryl s200 gel permeation. the catalytic activity of the purified enzyme required the presence of fructose-1,6-diphosphate with a broad opt ... | 1985 | 3917978 |
| transformation and fusion of streptococcus faecalis protoplasts. | nonconjugative plasmids were transferred by protoplast fusion among streptococcus faecalis strains and from streptococcus sanguis to s. faecalis. s. faecalis protoplasts were also transformed with several different plasmids, including the tn917 delivery vehicle ptv1. transformation was reproducible, but low in frequency (10(-6) transformants per viable protoplast). a new shuttle vector (pam610), able to replicate in escherichia coli and s. faecalis, was constructed and transformed into s. faecal ... | 1985 | 3920209 |
| leukocyte migration inhibition in recurrent aphthous ulceration. | leukocyte inhibitory factor (lif) production in response to streptococcal antigens and oral mucosa was examined as a possible pathogenic mechanism in recurrent aphthous ulceration (rau). mononuclear cells from rau patients and controls were stimulated with antigens prepared from two species of streptococci and from oral mucosa. candida albicans and foreskin were tested as control antigens. there were some statistically significant differences in leukocyte migration inhibition between patients an ... | 1985 | 3920363 |
| in-vitro inhibition of mycobacteria by viridans streptococci. | eleven strains of mycobacterium tuberculosis and 13 other species of mycobacteria were examined for susceptibility to 26 strains of viridans streptococci. susceptibility was detected by the simultaneous antagonism technique, but not by tests of deferred antagonism. it is concluded that in-vitro inhibition of mycobacterial growth by actively growing streptococci is due to a peroxide-mediated antagonism which is too variable to be of value for identification. however, a standard bactericidal test ... | 1985 | 3920400 |
| bactericidal activity of human lysozyme, muramidase-inactive lysozyme, and cationic polypeptides against streptococcus sanguis and streptococcus faecalis: inhibition by chitin oligosaccharides. | the basis of the bactericidal activity of human lysozyme against streptococcus sanguis was studied. experiments were designed to evaluate the role of lysozyme muramidase activity in its bactericidal potency. inactivation of the muramidase activity of lysozyme was achieved by reduction of essential disulfides with dithiothreitol (dtt) or by incubation with the chitin oligosaccharides chitotriose and chitobiose. muramidase-inactive lysozyme, prepared by reduction with dtt, was equal in bactericida ... | 1985 | 3922894 |
| characterization and molecular cloning of cryptic plasmids isolated from lactobacillus casei. | four small cryptic plasmids were isolated from lactobacillus casei strains, and restriction endonuclease maps of these plasmids were constructed. three of the small plasmids (plz18c, plz19e, and plz19f1; 6.4, 4.9, and 4.8 kilobase pairs, respectively) were cloned into escherichia coli k-12 by using pbr322, pacyc184, and puc8 as vectors. two of the plasmids, plz18c and plz19e, were also cloned into streptococcus sanguis by using pva1 as the vector. hybridization by using nick-translated cloned 32 ... | 1985 | 3923929 |
| comparison of single doses of amoxicillin or of amoxicillin-gentamicin for the prevention of endocarditis caused by streptococcus faecalis and by viridans streptococci. | recent recommendations for the prophylaxis of endocarditis in humans have advocated single doses or short courses of antibiotic combinations (beta-lactam plus aminoglycoside) for susceptible patients in whom enterococcal bacteremia might develop or for patients at especially high risk of developing endocarditis (e.g., patients with prosthetic cardiac valves). we tested the prophylactic efficacy (in rats with catheter-induced aortic vegetations) of single doses of amoxicillin plus gentamicin agai ... | 1985 | 3925031 |
| antistreptococcal activity of sch 34343, a new penem antibiotic. | the in-vitro activity of sch 34343 was compared with that of penicillin and cefotaxime against clinical isolates of streptococci. sch 34343 was highly active against streptococcus pneumoniae, viridans streptococci, streptococci of groups a, b, c and g, and str. bovis. ninety per cent of these strains were inhibited by less than or equal to 0.25, less than or equal to 0.12 and less than or equal to 0.06 mg/l of sch 34343, penicillin and cefotaxime respectively. activity of sch 34343 against str. ... | 1985 | 3928578 |
| lymphocyte function in recurrent aphthous ulceration. | lymphocyte blast transformation responses to mitogens and to streptococcal, adenovirus type 1, and oral mucosal antigens were studied in 32 patients with recurrent aphthous ulceration (rau) and matched control subjects. rau patients showed significantly depressed (p less than 0.05) responses to mitogens in comparison with matched controls, but there was no significant difference between patients and controls in response to any of the antigens tested. these results indicate that neither cell-medi ... | 1985 | 3930669 |
| genetic transformation in streptococcus sanguis. identification, surface spreading and competence of invasive strains of streptococcus sanguis lancefield groups h and w and other invasive viridans streptococci. | of 46 viridans streptococci isolated from cases of probably true bacteremia, 26 strains or 56% could be grouped serologically in the lancefield system. eight strains (17%) were identified as serogroup h (characteristic of streptococcus sanguis), ten strains (22%) as serogroup w (a new group antigen found in s. sanguis) and eight strains (17%) as serogroup k (related to s. mitis (mitior)). the high number of serogroupable viridans streptococci was specially due to the introduction of serogroup w ... | 1985 | 3931412 |
| chemical and immunological characterization of a novel amphipathic antigen from biotype b streptococcus sanguis. | a new type of amphipathic antigen was extracted from whole cells of streptococcus sanguis atcc 10557 (biotype b, serotype ii) by the phenol/water method. the extract was treated with nuclease p1, and was applied to a column of sepharose 6b. each fraction was checked by passive haemagglutination (pha) and immunodiffusion tests against anti-10557 serum which was obtained by immunizing rabbits with whole cells of strain atcc 10557. strong pha activity was demonstrated in the first hexose-containing ... | 1985 | 3932593 |
| n-methyl transferase of streptomyces erythraeus that confers resistance to the macrolide-lincosamide-streptogramin b antibiotics: amino acid sequence and its homology to cognate r-factor enzymes from pathogenic bacilli and cocci. | the nucleotide sequence of a structural gene erme for ribosomal rna (rrna) n6-amino adenine n-methyl transferase (nmt) of streptomyces erythraeus, cloned by thompson et al. [gene 20 (1982) 51-62], has been determined. the nmt amino acid (aa) sequence deduced from the nucleotide sequence contains extensive homology to aa sequences of cognate nmts specified by: (1) plasmid pe194 from staphylococcus aureus, 30% g + c, ermc; (2) plasmid pam77 from streptococcus sanguis, 43% g + c; as well as to (3) ... | 1985 | 3934045 |
| effects of oxygen on pyruvate formate-lyase in situ and sugar metabolism of streptococcus mutans and streptococcus sanguis. | the strictly anaerobic metabolism of sugar in strains of streptococcus mutans and streptococcus sanguis was studied because deep layers of dental plaque are strictly anaerobic. galactose-grown cells of these streptococcal strains had higher pyruvate formate-lyase activity than did glucose-grown cells. among these strains, two strains of s. mutans had a significantly higher pyruvate formate-lyase activity than did the others. this enzyme is extremely sensitive to oxygen, and even in situ the enzy ... | 1985 | 3965391 |
| surface structures (peritrichous fibrils and tufts of fibrils) found on streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera. | we screened 36 strains of streptococcus sanguis biotype i and 8 strains of s. sanguis biotype ii for the presence of surface structures and for their ability to coaggregate with actinomyces viscosus, actinomyces naeslundii, and fusobacterium nucleatum. negative staining under an electron microscope revealed detectable surface structures on all s. sanguis strains. the majority of strains (38 of 44) carried peritrichous fibrils, which have an irregular profile and no distinct width. they usually a ... | 1985 | 3965396 |
| effects of exopolysaccharide production by viridans streptococci on penicillin therapy of experimental endocarditis. | the influence of exopolysaccharide production by viridans streptococci on penicillin therapy of endocarditis was studied in rabbits with experimental aortic-valve endocarditis. animals were first infected with penicillin-susceptible streptococci known to produce varying amounts of exopolysaccharide in cardiac vegetations. treatment with procaine penicillin, 300,000 u every 12 hr, was begun one to two days after infection. after five days of therapy, animals infected with streptococcus sanguis ii ... | 1985 | 3965587 |
| recombination-deficient streptococcus sanguis. | a uv-sensitive derivative was obtained from streptococcus sanguis challis. the organism could be transformed with a number of small streptococcal plasmids at frequencies equal to, or 1 logarithm below, the transformation frequencies for the parent organism. however, transformation with chromosomal dna was greatly impaired in the uv-sensitive derivative. | 1985 | 3988349 |
| serological identification of streptococcus sanguis and str mitior. | a total of 165 strains of streptococcus sanguis and str mitior were selected on the basis of their biochemical reactions using established identification procedures. these strains were also classified using api database and were then screened against five candidate grouping sera. biochemical tests and serological identification were in general complementary, but no regular associations between biotype and serological reaction were observed. | 1985 | 3988957 |
| phosphatidyl glycerolphosphate serves as glycerolphosphate donor in polymer synthesis. | phosphatidyl glycerolphosphate was found to serve as the glycerolphosphate donor for polymer synthesis. when cdp-diglyceride and radiolabeled glycerolphosphate were incubated with the membrane enzyme prepared from streptococcus sanguis, active syntheses of radiolabeled lipids and polymers were observed. the synthesis of polymer was not inhibited by low concentration of unlabeled phosphatidylglycerol. when [3h, 32p]glycerolphosphate was used, the polymer synthesized contained both 3h and 32p. the ... | 1985 | 3994726 |
| 'hot' and 'cold' lesions detected by gallium-67 scintigraphy in a pyogenic liver abscess. | most pyogenic liver abscesses appear to be 'not' in a 67ga-citrate study; rarely, a 'warm' or 'cold' area may also be seen. we present a patient with a pyogenic liver abscess that had both 'cold' and 'hot' areas in the 67ga-citrate study. this striking finding is probably related to the different stages of evolution of the abscess. | 1985 | 3996439 |
| cloned gtfa gene of streptococcus mutans lm7 alters glucan synthesis in streptococcus sanguis. | streptococcus mutans lm7 (bratthall serotype e) chromosomal dna was partially digested with ecori and ligated into the positive-selection plasmid vector pop203(a2+). the ligation mixture was transformed into escherichia coli, and transformants were selected for tetracycline resistance. recombinant-bearing clones were screened for their ability to ferment raffinose, using the procedure of robeson et al. (j. bacteriol. 153:211-221, 1983). one raffinose-fermenting clone was isolated and found to co ... | 1985 | 3997243 |
| adhesion of streptococcus sanguis ch3 to polymers with different surface free energies. | the adhesion of the oral bacterium streptococcus sanguis ch3 to various polymeric surfaces with surface free energies (gamma s) ranging from 22 to 141 erg cm-2 was investigated. suspensions containing nine different bacterial concentrations (2.5 x 10(7) to 2.5 x 10(9) cells per ml) were used. after adhesion for 1 h at 21 degrees c and a standardized rinsing procedure, the number of attached bacteria per square centimeter (nb) was determined by scanning electron microscopy. the highest number of ... | 1985 | 4004241 |
| isolation of actinomyces bacteriophage from human dental plaque. | human dental plaque samples were screened for the presence of bacteriophage for actinomyces viscosus and streptococcus sanguis. none of the 336 samples yielded phage for s. sanguis, but 10 contained virulent actinomyces phage. a high host cell specificity was observed in that one phage isolate infected only a. viscosus t14v, eight phage isolates infected only a. viscosus mg-1, and one infected both strains. none was capable of productively infecting various other actinomyces strains that represe ... | 1985 | 4008044 |
| resistance in oral streptococci after repeated three-dose erythromycin prophylaxis. | ten healthy volunteers, from whom no erythromycin-resistant oral streptococci could be isolated initially, received three doses of erythromycin stearate (1.0, 0.5 and 0.5 g) on two separate occasions with a one-week interval between them. after the second administration all volunteers yielded moderately resistant strains (mic 1-4 mg/l) and four harboured highly resistant streptococci (mic 16- greater than 256 mg; mbc 128- greater than 256 mg/l). erythromycin-resistant strains persisted in eight ... | 1985 | 4008379 |
| extracellular matrix proteins (fibronectin, laminin, and type iv collagen) bind and aggregate bacteria. | the normal microbial colonization of sites in the body's tissues by certain bacteria requires that the bacteria first bind to extracellular secreted constituents, cell-surface membranes, or cell matrixes. this study examines two interactions of a variety of bacteria with the cell matrix noncollagenous proteins fibronectin and laminin and with basement membrane (type iv) collagen. adherence of bacteria to matrix proteins coated on tissue culture wells was examined with the use of radiolabeled bac ... | 1985 | 4014440 |
| [spondylodiscitis after embolization of an extramedullary intraspinal arteriovenous fistula]. | the authors report a case of spondylitis th11-th12 occurred 1 month after embolization of an intraspinal extramedullary arteriovenous fistulae; this fistulae was fed by 11th left intercostal artery. the infecting organism isolated from the affected intervertebral disc was streptococcus sanguis a common agent of dental abscess. | 1985 | 4020738 |
| indifferent streptococci in normal and purulent eyes of neonates. | the incidence of indifferent streptococci in the eyes of neonates less than 6 days old was investigated. the isolation of indifferent streptococci was significantly higher in infants with sticky eyes. speciation using a shortened identification scheme was carried out on isolates; streptococcus mitior and streptococcus sanguis were the most common species in both purulent and non-infected eyes. streptococcus mutans, a species not normally found in edentulous infants, comprised 14% of indifferent ... | 1985 | 4031105 |
| enhanced killing of penicillin-treated gram-positive cocci by human granulocytes: role of bacterial autolysins, catalase, and granulocyte oxidative pathways. | staphylococci pretreated with subminimal inhibitory concentrations (submic) of cell-wall active antibiotics exhibit increased susceptibility to killing by human polymorphonuclear leukocytes (pmns), even when phagosome information is impaired by the mold metabolite, cytochalasin b. to investigate the role of specific bacterial factors in the process, studies were carried out with organisms lacking catalase (streptococci) or cell-wall autolytic enzymes and compared to findings with staphylococcus ... | 1985 | 4036175 |
| cell surface components of streptococcus sanguis: relationship to aggregation, adherence, and hydrophobicity. | cell surfaces of aggregation, adherence, and hydrophilic variants of streptococcus sanguis were compared with cell surfaces of the parent strain with regard to their protein and antigenic constituents. cell surface molecules were released by digestion with mutanolysin. extraction with sodium dodecyl sulfate (sds) urea, lithium diiodosalicylate, and boiling water did not solubilize any material which stained with agno3 in an sds-polyacrylamide gel electrophoresis gel. the parent organism s. sangu ... | 1985 | 4044521 |
| genetic transformation in streptococcus sanguis. effects on genetic transformation by culture filtrates of streptococcus sanguis (serogroups h and w) and streptococcus mitis (mitior) with reference to identification. | streptococcus sanguis (serogroups h and w) strains are frequently competent in genetic transformation. in a collection of 18 strains of s. sanguis isolated from blood cultures, 12 strains were spontaneously competent in transformation by streptomycin-resistant dna, and three strains were induced to competence by the use of culture filtrates containing competence factor (cf). culture filtrates from spontaneously competent s. sanguis strains only induced competence in transformation of strains of ... | 1985 | 4050439 |
| regulation of glycolytic rate in streptococcus sanguis grown under glucose-limited and glucose-excess conditions in a chemostat. | the biochemical mechanisms of the acidogenic potential of streptococcus sanguis atcc 10556 grown in glucose-excess and glucose-limited continuous culture were studied. the rate of acid production during the glucose metabolism by the cells grown under glucose limitation (glucose-limited cells) was 2.1 to 2.6 times that by the cells grown in an excess of glucose (glucose-excess cells). when the glucose-limited cells were metabolizing glucose, intracellular concentrations of glucose 6-phosphate, fr ... | 1985 | 4055023 |
| comparative studies on the effect of growth conditions on adhesion, hydrophobicity, and extracellular protein profile of streptococcus sanguis g9b. | streptococcus sanguis g9b was grown in continuous culture at different generation times and ph values in media containing either glucose or fructose and differing in the concentrations of na+ and k+. the growth ph, carbohydrate, and cation concentration each affected the yield of organisms, their ability to adhere to saliva-coated hydroxyapatite beads, and their hydrophobicity, as measured by adhesion to hexadecane. there was no correlation between adhesion to saliva-coated hydroxyapatite beads ... | 1985 | 4055033 |
| albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles. | a procedure involving blocking with bovine albumin was useful for differentiating between streptococcal interactions with components of experimental salivary pellicles similar to those which form on teeth and potential interactions with uncoated areas of mineral. | 1985 | 4055036 |
| characterization of nutritionally variant streptococci by biochemical tests and penicillin-binding proteins. | a comparative study of 60 strains of nutritionally variant streptococci (nvs) with 34 strains of streptococcus mitis and 37 strains of streptococcus sanguis ii showed the presence of a red chromophore which was absent in the other streptococcal species. by using the conventional microbiological tests, only small differences were found between the nvs and the two other related species. in contrast a clear-cut delineation was found by the api 20 strep system of identification. all nvs contained py ... | 1985 | 4066913 |
| bacterial meningitis following pantopaque myelography. a case report and literature review. | a case of acute bacterial meningitis following pantopaque myelography is reported, and the literature reviewed pertaining to this uncommon but potentially fatal complication. a positive gram's stain is most helpful in differentiating bacterial from chemical meningitis. treatment of the meningitis before and after determination of its cause is described. preventive steps include removal of pantopaque from the subarachnoid space immediately at the conclusion of fluoroscopy, and observance of stric ... | 1985 | 4094954 |
| myocardial depression and nephrotic syndrome in streptococcus sanguis endocarditis. | nephrotic syndrome is a most unusual complication of infective endocarditis. we report a case of streptococcus sanguis endocarditis of the tricuspid valve in a patient with a small congenital ventricular septal defect who developed this complication. severe myocardial depression also occurred and the clinical behaviour and subsequent recovery of this closely paralleled that of the nephrotic syndrome. these unusual associations, their possible interactions and pathogeneses are discussed. | 1985 | 4095256 |
| competence-specific autolysis in streptococcus sanguis. | streptococcus sanguis strain wicky activated to competence for genetic transformation is known to undergo a rapid decrease in optical density upon transfer to an alkaline buffer containing reducing agents. we studied the mechanism of this autolysis-like process and made the following observations. the process was specific because preincubation of the competence inducing factor with a specific inactivating protein prevented both cellular lysis and acquisition of competence for genetic transformat ... | 1985 | 2410542 |
| the phylogenetic position of streptococcus and enterococcus. | streptococcus pyogenes, s. equinus, s. bovis, s. salivarius, s. sanguis, s. mutans, s. rattus, s. cricetus, s. lactis, s. raffinolactis and enterococcus faecalis have been characterized by oligonucleotide cataloguing of their 16s ribosomal rna. all the organisms form a loose but coherent group that is phylogenetically equivalent to those of lactobacilli, bacilli, the brochothrix and listeria group, and related taxa that constitute one of several sublines within the 'clostridium' branch of gram-p ... | 1985 | 2410543 |
| reduction of acidurance of streptococcal growth and glycolysis by fluoride and gramicidin. | the acidurance of glycolysis by intact cells of streptococcus mutans gs-5, streptococcus salivarius atcc 25925, and streptococcus sanguis nctc 10904 was found to be highly dependent on membrane functions affected by gramicidin, which increases the proton permeability of cell membranes. plots of % glucose utilized during two hours against suspension ph values for cells suspended in 100 mm phosphate buffer plus 1 mm mgcl2 plus 13.9 mm glucose indicated, for 50% glucose utilization, ph values of 5. ... | 1985 | 2579114 |
| the effects of basic and acidic synthetic polypeptides on the adherence of the oral bacteria, streptococcus mutans and streptococcus sanguis, to hydroxyapatite. | two basic and two acidic synthetic polypeptides that bind strongly to hydroxyapatite at neutral ph were tested to determine their influence on adsorption of two streptococcus mutans and two streptococcus sanguis strains to hydroxyapatite. the adsorption of the strains was significantly enhanced or reduced by the basic and acidic agents, respectively. study of acidic polypeptides provided evidence of competition between the polypeptides and the bacterial cells for hydroxyapatite adsorption sites. | 1985 | 2581534 |
| surface properties of streptococcus sanguis fw213 mutants nonadherent to saliva-coated hydroxyapatite. | seventeen mutants of streptococcus sanguis fw213 nonadherent to saliva-coated spheroidal hydroxyapatite were isolated after mutagenesis with ethyl methanesulfonate, nitrosoguanidine, nitrous acid, hydroxylamine, or 2-aminopurine. enrichment for nonadherent mutants was accomplished by successive adsorptions of the adherent strains to saliva-coated hydroxyapatite. after enrichment, variant colonial morphology on tryptic agar was used as a screening technique for selection of nonadherent mutants, w ... | 1985 | 2857684 |
| antibodies that bind to fimbriae block adhesion of streptococcus sanguis to saliva-coated hydroxyapatite. | antibodies raised against a fimbriated, adhesive strain of streptococcus sanguis (fw213) were found to block the adhesion of this organism to saliva-coated hydroxyapatite. antibodies were made specific for adhesion antigens by adsorption with isogenic, nonadhesive mutants (for rabbit polyclonal adsorbed antibody) or selection based on nonreactivity with two nonadhesive mutants (for monoclonal antibody). rabbit antibody raised against isogenic, nonfimbriated nonadhesive mutants served as a contro ... | 1985 | 2860066 |
| genetic transformation in streptococcus sanguis. | 1985 | 2864407 | |
| salivary-iga antibody to glucosyltransferase of oral microbial origin in children. | salivary iga levels were measured in whole saliva of 143 children, 2-48 months of age. the median salivary iga concentration of children less than 12 months old was 30 micrograms/ml. a significant increase in salivary iga concentration occurred in children 12 to 17 months old. more than 50 per cent of the population, 36 months of age and older, had streptococcus mutans (88 per cent biotype i) recoverable from their teeth. salivary iga antibody levels were measured by an enzyme-linked immunosorbe ... | 1985 | 2933016 |
| interaction of deoxyhalosucrose derivatives with dextransucrase. | members of a series of deoxyhalosucrose analogs substituted at one, two, or three primary carbon atoms with bromine or chlorine were prepared. dextransucrase isolated from streptococcus sanguis was separately treated with 6-bromo-6-deoxysucrose, 6,6'-dibromo-6,6'-dideoxysucrose, 6,1',6'-tribromotrideoxysucrose, and 6,6'-dichlorodideoxysucrose, in order to determine if they were inactivators. variation in time of exposure, and in the concentration of the sucrose analogs, did not yield significant ... | 1985 | 2934130 |
| purification, and comparison, of two forms of dextransucrase from streptococcus sanguis. | a procedure has been developed whereby native and proteolyzed forms of dextransucrase have been purified; it involves gel filtration, and hydroxylapatite chromatography in the presence of 0.10% sodium dodecyl sulfate. this procedure is highly reproducible, and permits approximately 30% recovery of high purity (94% homogeneous) enzyme as an inactive, sds complex that can be reactivated by the addition of triton x-100. the purified enzymes have been compared with regard to amino acid compositions, ... | 1985 | 2934131 |
| a conjugative transposon (tn919) in streptococcus sanguis. | streptococcus sanguis fc1, originally isolated from dental plaque, was found to be naturally resistant to tetracycline. although no plasmid dna could be detected, tetracycline resistance was transferable in filter matings to streptococcus faecalis fa2-2. again, no plasmid dna was detectable in transconjugants, and the latter could donate tetracycline resistance to s. faecalis, s. sanguis, and streptococcus lactis. the tetracycline resistance element was able to transpose to several sites on the ... | 1985 | 2981772 |
| contribution of stereochemical interactions in the adhesion of streptococcus sanguis c5 to experimental pellicles. | parameters for the adsorption of streptococcus sanguis c5 to experimental salivary pellicles were determined to assess the relative contributions of stereochemical and less specific hydrophobic-electrostatic interactions in this process. s. sanguis c5 cells possess hydrophobic surfaces and also an adhesin which exhibits stereochemical binding to sialic acid-containing salivary receptors. the adsorption of s. sanguis c5 to untreated pellicles was significantly better described by a two-site adsor ... | 1985 | 2982936 |
| nucleotide sequence of erma, a macrolide-lincosamide-streptogramin b determinant in staphylococcus aureus. | the complete nucleotide sequence of erma, the prototype macrolide-lincosamide-streptogramin b resistance gene from staphylococcus aureus, has been determined. the sequence predicts a 243-amino-acid protein that is homologous to those specified by ermc, ermam, and ermd, resistance determinants from staphylococcus aureus, streptococcus sanguis, and bacillus licheniformis, respectively. the erma transcript, identified by northern analysis and s1 mapping, contains a 5' leader sequence of 211 bases w ... | 1985 | 2985541 |
| molecular cloning of a pip501 derivative yields a model replicon for the study of streptococcal conjugation. | we have previously constructed a derivative of the broad host range streptococcal plasmid pip501, a conjugative plasmid designated pva797, that confers chloramphenicol resistance and contains a unique ecori site in a non-essential region of the plasmid molecule. pva797 (30.7 kb) when cloned in toto as an ecori fragment into the positive selection vector pop203(a2+) gave a recombinant, pva904 (37.7 kb), which was able to replicate in escherichia coli and in streptococcal species. it can be phenot ... | 1985 | 2985739 |
| streptococcus faecalis sex pheromone (cam373) also produced by staphylococcus aureus and identification of a conjugative transposon (tn918). | streptococcus faecalis rc73 was found to harbor a conjugative plasmid (pam373) which confers a mating response to a sex pheromone (cam373) excreted by plasmid-free members of the same species. the pheromone was also detected in culture filtrates of all of 23 staphylococcus aureus strains but in only 2 of 22 coagulase negative staphylococcus strains. streptococcus sanguis challis and g9b also produced the activity, but 10 other streptococcus sanguis strains did not. the activity was also produced ... | 1985 | 2987186 |
| streptococcus faecalis r plasmid pjh1 contains a pam alpha 1 delta 1-like replicon. | streptococcus faecalis r plasmid pjh1 did not transform competent strains of streptococcus sanguis. a hybrid plasmid, pdl310, consisting of virtually all of the s. faecalis hemolysin-bacteriocin plasmid pjh2 and a segment of pjh1 dna that included the tetracycline resistance determinant, yielded tetracycline-resistant transformants at a frequency of less than 10(-8) transformants per cfu, when it was added to a competent culture of s. sanguis wicky. four of the transformants contained a 4.7-kilo ... | 1985 | 2997123 |
| complete nucleotide sequence of macrolide-lincosamide-streptogramin b-resistance transposon tn917 in streptococcus faecalis. | streptococcus faecalis transposon tn917 was cloned in escherichia coli on plasmid vector pbr325. the erythromycin resistance determinant of tn917 was not expressed in the e. coli background. the nucleotide sequence of tn917 was determined and found to be 5,257 base pairs in length. six open reading frames (orfs) were identified and designated 1 through 6 (5' to 3'); all were on the same dna strand. a region exhibiting strong homology with known promoters was identified upstream from orf1. orfs 1 ... | 1985 | 2997130 |
| environmental regulation of carbohydrate metabolism by streptococcus sanguis nctc 7865 grown in a chemostat. | carbohydrate metabolism by the oral bacterium streptococcus sanguis nctc 7865 was studied using cells grown in a chemostat at ph 7.0 under glucose or amino acid limitation (glucose excess) over a range of growth rates (d = 0.05 h-1-0.4 h-1). a mixed pattern of fermentation products was always produced although higher concentrations of lactate were formed under amino acid limitation. analysis of culture filtrates showed that arginine was depleted from the medium under all conditions of growth; a ... | 1985 | 2999295 |
| ph change in artificial dental plaque formed by glucosyltransferase and some oral bacteria during batch and continuous culture. | streptococcus mutans alone or cell-free glucosyltransferase (g-tase)-together with either streptococcus sanguis, streptococcus salivarius, actinomyces viscosus, or lactobacillus casei cells-formed artificial dental plaque that firmly adhered to glass electrodes in a continuous culture system containing sucrose. the ph in these artificial plaque samples decreased more than did that of the surrounding medium. in the absence of gtase, the bacteria other than s. mutans did not form firmly-adhering p ... | 1985 | 3156164 |
| immune complex hyperlipidemia induced by an apolipoprotein-reactive immunoglobulin a paraprotein from a patient with multiple myeloma. characterization of this immunoglobulin. | an antibodylike paraprotein has been isolated from a patient with multiple myeloma and autoimmune hyperlipoproteinemia. the paraprotein bound to apolipoprotein b (apo b)-containing lipoproteins that formed macromolecular aggregates, and globules thought to be aggregated complexes of lipoproteins and reactive immunoglobulins were observed circulating within the retinal blood vessels of this patient. this binding specificity permitted purification of the paraprotein from both the agglutinated immu ... | 1985 | 3160725 |
| ion extrusion systems in bacteria. | 1985 | 2418727 | |
| adherence of streptococcus sanguis to hydroxyapatite coated with lysozyme and lysozyme-supplemented saliva. | the adherence of [3h]thymidine-labeled streptococcus sanguis strains to bare hydroxyapatite and to hydroxyapatite coated with a range of concentrations of lysozyme, poly-l-lysine, poly-l-glutamic acid, whole saliva supernatant, and combinations of some of the above was studied. adherence of several strains of s. sanguis to bare hydroxyapatite and saliva-coated hydroxyapatite was compared. saliva present as a pellicle on the hydroxyapatite inhibited adherence of some strains (903, m-5, 73x11) and ... | 1986 | 2419251 |
| strains of streptococcus mutans and streptococcus sobrinus attach to different pellicle receptors. | we compared the levels of adsorption of streptococcus mutans jbp and streptococcus sobrinus 6715 to experimental pellicles formed from unsupplemented and glucosyltransferase (gtf)-supplemented saliva. pellicles formed on hydroxyapatite beads from gtf or from saliva-gtf mixtures possessed detectable gtf activity. low levels of gtf activity were also detected in clarified whole human saliva, but not in samples of submandibular saliva. the adsorptive behavior of s. mutans jbp to pellicles formed fr ... | 1986 | 2422126 |
| comparative adhesion of seven species of streptococci isolated from the blood of patients with sub-acute bacterial endocarditis to fibrin-platelet clots in vitro. | the adhesion to fibrin-platelet clots in vitro of 21 strains of streptococci isolated from the blood of patients with sub-acute bacterial endocarditis (sabe) was measured. the species, in order of greatest adhesion, were streptococcus faecalis, streptococcus mutans, streptococcus milleri, streptococcus sanguis, dextran-positive streptococcus mitior, dextran-negative streptococcus mitior and streptococcus salivarius. individual strains within species, however, cannot be assumed to be representati ... | 1986 | 2422150 |
| monoclonal antibodies against isotypic and isoallotypic determinants of human iga1 and iga2: fine specificities and binding properties. | we have analysed and compared the fine specificity and behavior in various immunoassays of 10 mouse monoclonal antibodies, from three independent laboratories, directed against iga1, iga2 or non-iga2m(2). the following observations were made. (1) although all of the monoclonal antibodies were specific for a particular iga subclass or isoallotype in a radioimmunoassay, three of them were not specific when tested in indirect immunofluorescence on plasma cells derived from pokeweed-activated periph ... | 1986 | 2425248 |
| cell-surface proteins of streptococcus sanguis associated with cell hydrophobicity and coaggregation properties. | incubating cells of streptococcus sanguis with sodium lauroyl sarcosinate, under conditions that did not cause lysis, solubilized material comprising 5-8% of the cell dry weight. the treatment reduced cell hydrophobicity, and reduced the ability of the cells to coaggregate with actinomyces spp. the extract contained about 20 polypeptides and these were identified as being cell-surface components on the basis of one or more of the following criteria: being degraded when cells were incubated with ... | 1986 | 2433382 |
| isolation and characterization of a 60-kilodalton salivary glycoprotein with agglutinating activity against strains of streptococcus mutans. | a bacterial agglutinin specific for strains of streptococcus mutans was isolated from human saliva. physiochemical analyses showed the agglutinin to be a glycoprotein with a molecular weight of 60,000. the agglutinin aggregated four of the eight strains of streptococcus mutans tested but did not aggregate the strains of streptococcus salivarius, streptococcus sanguis, and streptococcus mitis tested. chemical modification of carbohydrate moieties of the agglutinin with sodium metaperiodate had no ... | 1986 | 3002983 |
| highly efficient protoplast transformation system for streptococcus faecalis and a new escherichia coli-s. faecalis shuttle vector. | a highly efficient protoplast transformation system for streptococcus faecalis has been developed by systematically optimizing different parameters. up to 10(6) transformants per micrograms of dna were consistently obtained within 3 days, and cell wall regeneration of protoplasts was virtually 100%. a systematic search for useful vectors showed that the broad-host-range plasmid pip501 could transform s. faecalis at a high frequency (6.3 x 10(4) transformants per microgram). by combining a high-c ... | 1986 | 3005240 |
| infective endocarditis presenting with back pain and stroke. | 1986 | 2939151 | |
| some remarks about laser-induced mass spectrometry of bacteria. | in order to investigate the availability of laser-induced mass spectrometry for the determination of bacteria, ten strains of the genera escherichia and streptococcus were chosen. gram-negative as well as gram-positive lysogenic and non-lysogenic, pathogenic and non-pathogenic strains were examined. giant pulses of a nd: yag laser (frequency quadrupled, wave-length 265 nm) were used to vaporize and partly ionize the bacterial material. the time-of-flight mass spectra obtained could be discrimina ... | 1986 | 2939649 |
| dynamic reactivities of dextransucrase. | dextransucrase, from streptococcus sanguis atcc 10558, was immobilized on hydroxylapatite and was "charged" in short pulses with labeled sucrose, as previously described [v. k. parnaik, g. a. luzio, d. a. grahame, s. l. ditson, and r. m. mayer (1983) carbohydr. res. 121, 257-268]. the "charged" enzyme has been shown to contain both bound glucose and gluco-oligosaccharides. the reactivity of this form of the enzyme has been studied, and shown to have unexpected behavior. earlier pulse-chase exper ... | 1986 | 2943225 |
| teicoplanin-induced ototoxicity in down's syndrome. | 1986 | 2869320 | |
| immunological study of lactate dehydrogenase from streptococcus mutans and evidence of common antigenic domains with lactate dehydrogenases from lactic bacteria. | rabbit polyclonal antibodies directed against purified streptococcus mutans l-(+)-lactate dehydrogenase reacted with the purified enzyme, giving a marked deviation of its kinetic parameters. the enzyme affinity for pyruvate or nadh decreased in the presence of antibody, the affinity for fructose 1,6-diphosphate (fdp) appeared to be slightly affected, and the cooperativity of the ligand binding was lowered. a partial protective effect was observed when the enzyme was preincubated with fdp prior t ... | 1986 | 2416689 |
| variable colonization by oral streptococci in molar fissures of monoinfected gnotobiotic rats. | germfree sprague-dawley rats, fed a high-sucrose diet, were monoinfected with strains of streptococcus mitis, streptococcus sanguis, and streptococcus mutans. viable cell recoveries from six molar teeth, considered to reflect mainly bacterial colonization of intact fissures, were in the order of 10(6), 10(7), and 10(8) cfu, respectively. some of the implications of the variation of bacterial plaque-forming ability in the rat model are discussed. | 1986 | 3699897 |
| use of the photoaffinity cross-linking agent n-hydroxysuccinimidyl-4-azidosalicylic acid to characterize salivary-glycoprotein-bacterial interactions. | the present study has utilized the iodinatable cross-linking agent n-hydroxysuccinimidyl-4-azidosalicylic acid (asa) to examine the specific interaction between the proline-rich glycoprotein (prg) of human parotid saliva and streptococcus sanguis g9b. the binding of 125i-asa-prg to streptococcus sanguis g9b displayed saturation kinetics, reversibility and was inhibited by unlabelled prg. inhibition studies with other glycoproteins and saccharides indicated that binding was mediated by a bacteria ... | 1986 | 3707547 |
| high-performance liquid chromatographic analysis of bacterial fatty acid composition for chemotaxonomic characterization of oral streptococci. | a high-performance liquid chromatographic method was developed to analyze the fatty acid composition of bacterial lipids. after saponification of lipids extracted from bacteria, the liberated fatty acids were labeled with a fluorescence reagent, 4-bromomethyl-7-acetoxycoumarin, followed by reversed-phase high-performance liquid chromatographic separation and fluorescence detection. all bacterial fatty acids were simultaneously separated within 30 min and sensitively determined. this method was a ... | 1986 | 3722368 |
| comparative capacity of orally administered amoxicillin and parenterally administered penicillin-streptomycin to protect rabbits against experimentally induced streptococcal endocarditis. | a single-intramuscular-dose immunization regimen with a penicillin g-streptomycin combination was compared with three oral-dose amoxicillin regimens for the capacity to prevent streptococcus sanguis infections of experimentally induced valvular heart lesions in rabbits. challenge doses of 10(4), 10(6), and 10(8) cfu of a strain of s. sanguis equally susceptible to penicillin and amoxicillin were used in this study. measured by recovery of test organisms from endocardial lesions, the lowest conce ... | 1986 | 3729348 |
| kinetics of adhesion of the oral bacterium streptococcus sanguis ch3 to polymers with different surface free energies. | the kinetics of adhesion of streptococcus sanguis ch3 from suspension to polymers with different surface free energies were studied by using three bacterial concentrations (2.5 x 10(7), 2.5 x 10(8), and 2.5 x 10(9) cells per ml-1). substratum surface free energies (gamma s) ranged from 18 to 120 erg cm-2. the kinetics of bacterial adhesion to these surfaces showed a typical two-step adhesion process, indicating an equilibrium in both steps. in the initial adhesion step (step 1), low equilibrium ... | 1986 | 3729392 |
| preparation of a sialic acid-binding protein from streptococcus mitis ks32ar. | a recent report has identified a lectin on the surfaces of several strains of streptococcus mitis and streptococcus sanguis with specificity for an n-acetylneuraminic acid alpha 2,3-galactose-beta 1,3-n-acetylgalactosamine sequence (p.a. murray, m.j. levine, l.a. tabak, and m.s. reddy, biochem. biophys. res. commun. 106:390-396, 1982). in the present study, purification and characterization of this sialic acid-binding protein (sabp) was begun. a clinical isolate of s. mitis was grown to mid stat ... | 1986 | 3733221 |
| graft infection and bacteremia with a tolerant l-form of streptococcus sanguis in a patient receiving hemodialysis. | i report a case of a tolerant l-form streptococcus sanguis infection involving an artificial vascular access site that was probably acquired from a nonhuman source; this is the first report of such an infection in a human. gram stains from the infected access site and blood cultures revealed an aberrant form which could only be recovered by passage through hypertonic sucrose media before being reisolated and subsequently identified as s. sanguis. the same organism was isolated from a pet dog of ... | 1986 | 3745426 |
| viridans streptococcal endocarditis: clinical, microbiological, and echocardiographic correlations. | infections caused by species within the viridans streptococci have been associated with different clinical characteristics. we studied 36 patients with viridans streptococcal endocarditis. complications were seen in 10 (32%) of 31 patients with native valve endocarditis and four (80%) of five with prosthetic valve endocarditis and included death in two, valve replacement in six, persistent infection in three, emboli in two, and congestive heart failure in nine. two-dimensional echocardiograms de ... | 1986 | 3745973 |
| xylitol metabolism in xylitol-sensitive and xylitol-resistant strains of streptococci. | the metabolism of xylitol in xylitol-sensitive strains (strains whose growth is inhibited by xylitol) and xylitol-resistant strains (growth not inhibited) of oral streptococci was compared. both xylitol-sensitive and xylitol-resistant strains took up xylitol. in the sensitive cells, the xylitol was probably transported via a phosphotransferase system. this resulted in intracellular accumulation of xylitol-5-phosphate and xylulose-5-phosphate. these metabolites were not detected in the xylitol-re ... | 1986 | 3751578 |
| possible pathogenic implications of right-sided polymicrobial endocarditis in a heroin abuser. | 1986 | 3758055 | |
| characterization of monoclonal antibodies specific for adhesion: isolation of an adhesin of streptococcus sanguis fw213. | monoclonal antibodies reactive to an adhesive strain of streptococcus sanguis (fw213) and nonreactive to a nonadhesive mutant (jl7) were derived from the fusion of myeloma line x63ag8.653 and spleen cells from balb/c mice immunized with live s. sanguis cells. five cell lines, belonging to subclasses of immunoglobulin g, produced monoclonal antibodies specifically directed against the adhesive strain. all five antibodies also failed to react with five additional, independently isolated, nonadhesi ... | 1986 | 3770949 |
| multigeneric aggregations among oral bacteria: a network of independent cell-to-cell interactions. | a radioactivity-based assay was developed to define the participation of radioactively labeled cell types within the milieu of unlabeled partners in multigeneric aggregates. the cell types in these multigeneric aggregations consisted of various combinations of 21 strains representing five genera of human oral bacteria. the coaggregation properties of each cell type, when paired individually with various strains, were delineated and were unchanged when the microbes took part in the more complex m ... | 1986 | 3782029 |
| salivary immunoglobulin a and serum antibodies to streptococcus mutans ribosomal preparations in dental caries-free and caries-susceptible human subjects. | caries-free subjects or individuals with low caries susceptibility exhibited significantly higher (p less than 0.001) levels of naturally occurring salivary immunoglobulin a (iga) and serum igg, iga, and igm antibodies to a streptococcus mutans ribosomal preparation than subjects with high caries susceptibility. absorption of saliva and serum samples with s. mutans ribosomal preparations, but not with other s. mutans antigens or with escherichia coli and neisseria gonorrhoeae ribosomal preparati ... | 1986 | 3941001 |
| enzyme-linked immunosorbent assay for the detection of antibodies to nutritionally variant streptococci in patients with endocarditis. | the viridans streptococci are responsible for 50%-55% of microbial endocarditis. among these varied species, the nutritionally variant streptococci (nvs) have recently been associated with culture-negative endocarditis and are responsible for 5%-10% of all streptococcal endocarditis. the isolation and identification of these bacteria has been hampered by the extremely fastidious nature of their growth requirements as well as by their slow growth rate. in addition, their antibiotic sensitivity va ... | 1986 | 3941275 |
| streptococcal endocarditis: single vs. combination antibiotic therapy and role of various species. | forty-eight patients with 51 episodes of infective endocarditis caused by streptococci were studied in a comparison of the efficacy of single antibiotic therapy versus that of combination therapy. there was no statistical difference in terms of clinical course, toxicity, and mortality between the group given a single drug and that given combination therapy. however, one patient experienced two relapses, in both instances after high-dose penicillin therapy. the species and antibiotic susceptibili ... | 1986 | 3952425 |
| active streptokinase from the cloned gene in streptococcus sanguis is without the carboxyl-terminal 32 residues. | the streptokinase expressed by the cloned gene in streptococcus sanguis has a molecular weight of about 44 000 [malke, h., gerlach, d., kohler, w., & ferretti, j.j. (1984) mgg, mol. gen. genet. 196, 360-365] while the molecular weight of the native streptokinase is 47 000. the structural and activity differences of the cloned streptokinase (csk) as expressed by s. sanguis and the native streptokinase (nsk) were investigated. from a partially purified csk, two active fractions were obtained by re ... | 1986 | 3954985 |
| alterations due to ampicillin and rifampicin in s. sanguis and s. aureus isolated from dental plaque. an electron microscopic study. | in this work we analysed the ultrastructural changes in s. aureus and s. sanguis after exposure to rifampicin and ampicillin respectively. s. aureus grown in the presence of rifampicin showed ultrastructural changes that can be summarized as follows: the peripheral cell-wall increased to three to five times the normal thickness; the cross-walls were considerably (two to ten times) thicker than normal; after 8 h of exposure a complete lysis occurred. s. sanguis grown in the presence of ampicillin ... | 1986 | 3955780 |
| characterization of genetic transformation in streptococcus mutans by using a novel high-efficiency plasmid marker rescue system. | we developed a marker rescue system for study of competence development and genetic transformation in streptococcus mutans. the system involved the recombinational rescue of a tetracycline resistance (tcr) determinant by a homologous, inactive locus (tcs because of a small deletion). streptococcal cells harboring this in vitro-prepared tcs construct (pva1208) were restored to tcr when plasmid (pva981) dna was used as donor material. pva981 contained the intact streptococcal tcr locus and was una ... | 1986 | 3009419 |
| broad geographical distribution of homologous erythromycin, kanamycin, and streptomycin resistance determinants among group d streptococci of human and animal origin. | the emr, kmr, and smr determinants of the streptococcus faecalis r plasmid pjh1 were cloned in streptococcus sanguis with a streptococcal plasmid vector, pva380-1. each cloned determinant was used as a probe in hybridization reactions with dot blots containing plasmid-enriched dna from 91 group d streptococcal isolates resistant to erythromycin, kanamycin, and streptomycin; the isolates were obtained from animal and human sources in a variety of geographical locations. nearly 70% of the strains ... | 1986 | 3010845 |
| acid tolerance, proton permeabilities, and membrane atpases of oral streptococci. | differences in acid tolerance among representative oral streptococci were found to be related more closely to the dynamic permeabilities of the bacteria to protons than to differences in the sensitivities of cell membranes to gross damage caused by environmental acidification. for streptococcus mutans gs-5, streptococcus sanguis nctc 10904, and streptococcus salivarius atcc 13419, gross membrane damage, indicated by the release of magnesium from whole cells, occurred at ph values below about 4 a ... | 1986 | 3015800 |
| coagglutination reactions between candida albicans and oral bacteria. | an agglutination assay for detecting intermicrobial adherence between the cells of candida albicans and various oral bacteria is described. strains of streptococcus sanguis, s. salivarius, s. mutans, s. mitis, fusobacterium nucleatum and actinomyces viscosus all coagglutinated with c. albicans. no interaction could be demonstrated between the cells of bacteroides melaninogenicus and those of c. albicans. preliminary investigations of these interactions suggest that binding of f. nucleatum and a. ... | 1986 | 3018251 |
| kinetic analysis of streptococcus sanguis adhesion to artificial pellicle. | studies of equilibria between streptococcus sanguis and artificial pellicle have suggested that there are multiple binding sites for the organism. in the present study, adhesion of s. sanguis to saliva-coated hydroxylapatite was examined by means of kinetic methods. cell-pellicle complex formation was measured from initiation of binding to equilibrium. rate constants were calculated for forward reactions (adsorption) and reverse reactions (desorption). initial binding obeyed reversible, first-or ... | 1986 | 3020104 |
| effects of highly concentrated stannous fluoride and chlorhexidine regimes on human dental plaque flora. | the aim of this study was to determine the effect of an intensive antimicrobial treatment on the number of streptococcus mutans, streptococcus sanguis, actinomyces viscosus/actinomyces naeslundii, and the total colony-forming units (cfu) in plaque. the dentition of human volunteers was treated in a dental office with either chlorhexidine (5%) or stannous fluoride (8%). following the office treatment with chlorhexidine, selected volunteers rinsed daily at home for seven or 49 days with chlorhexid ... | 1986 | 3455699 |
| characterization of human dental plaque formed on hydrogen-ion-sensitive field-effect transistor electrodes. | the purpose of this study was to evaluate (with scanning electron microscopy and microbiological characterization) the bacterial deposits which accumulate on hydrogen-ion-sensitive field-effect transistor electrodes (ph-isfet) under conditions normally employed for telemetric monitoring of changes in human dental plaque ph. electrodes were mounted in a carrier appliance which was worn for two, four, and six days. the plaque ph response to a sucrose solution increased with the age of the plaque, ... | 1986 | 3457051 |
| persistence and spread of the orally-implanted bacterium streptococcus sanguis between persons. | transformant strains of streptomycin-resistant streptococcus sanguis persisted 2.5 years after oral implantation in 6 out of 11 subjects. natural transmission of these bacteria to spouses had occurred in 5 of the 6 infected subjects. at follow-up examinations, the median value of streptomycin-resistant strep. sanguis per ml saliva was 226,000 c.f.u. in the infected subjects and 352,000 c.f.u. in their spouses. the median values of streptomycin-resistant strep. sanguis as a percentage of the tota ... | 1986 | 3458434 |
| enamel demineralization by acid produced from endogenous substrate in oral streptococci. | bovine enamel blocks were covered by a bacterial cell mass and held in the mouths of human volunteers by a palatal appliance, to study the effects of acid production from endogenous bacterial substrate on the ph of the mass and on enamel demineralization. variables were cells with and without endogenous substrate, rinsing with 10 per cent glucose or sucrose solution, or no rinsing and the type of organism. acid production from endogenous substrate by streptococcus mutans strain ib-1600, due, at ... | 1986 | 3459413 |
| secretory and serum antibody responses to oral bacterial antigens associated with recurrent aphthous ulceration. | 1986 | 3459829 | |
| the influence of diet on the growth of streptococcal bacteria on the molar teeth of monkeys (macaca fascicularis). | the rates of regrowth of bacteria in developmental grooves on these teeth were determined. plaque was removed from palatal grooves up to 96 h after cleaning and the number of bacteria and of individual streptococcal species were determined; streptococcus sanguis, streptococcus mutans and streptococcus mitior were the major ones. in monkeys fed one of three different diets, the numbers of each species reached stable levels 18-24 h after tooth cleaning. the median doubling times of the streptococc ... | 1986 | 3467667 |
| effect of monoclonal antibodies against lipoteichoic acid from the oral bacterium streptococcus mutans on its adhesion and plaque-accumulation in vitro. | five monoclonal antibodies directed against streptococcus mutans strain jbp lipoteichoic acid (lta) were characterized. they were all similarly reactive with the immunizing lta-containing extract, with intact strep. mutans jbp cells and with lta purified from lactobacillus casei. immobilized anti-lta antibodies removes lta from lta-containing extracts. the binding of antibodies to lta was inhibited by the aqueous extract but not by the organic extract of de-acylated lta, indicating reactivity wi ... | 1986 | 3467668 |
| safety and antibacterial properties of controlled release snf2. | two clinical studies were performed to evaluate the safety and efficacy of an intracoronal device for the controlled release of snf2. snf2-polycarboxylate cement, containing approximately 72 mg f-, was placed in subjects' molars requiring two-surface restorations. in the trial for safety, eight subjects had the restoration in place for 34 days. the salivary fluoride levels were elevated to a mean of 0.3/10(6) over the experimental period. urinary fluoride levels were only above baseline levels d ... | 1986 | 3511199 |
| effect of antimicrobial agents on root surface caries, alveolar bone loss, and microflora in rice rats. | two antimicrobial agents, 9-aminoacridine (0.2%) and minocycline (0.2%), were evaluated for their efficacy in inhibiting root surface caries, bone loss, and microflora in rice rats. a solution of 5000 ppm fluoride was used as a positive control for the inhibition of root surface caries, and double-distilled water was used as a negative control group. each rat was treated by having its molar teeth swabbed 2 x per day with the prescribed agent in its group for nine weeks. root caries reduction in ... | 1986 | 3517096 |
| differentiation of streptococcus sanguis and s. mitior by whole-cell rhamnose content and possession of arginine dihydrolase. | whole-cell rhamnose concentrations were measured in 48 strains of streptococci resembling streptococcus sanguis and s. mitior. physiological characteristics were tested by the api-20/strep system, and it was found that "typical" s. sanguis (arginine positive, aesculin positive) contained significant amounts of rhamnose, while "typical" s. mitior (arginine negative, aesculin negative) contained very low or undetectable amounts of rhamnose. both groups contained dextran-positive and dextran-negati ... | 1986 | 3517336 |