Publications

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a new measure of the sensitivity of electron spin resonance spectrometers. 20154300153
[pleurisy and cancer of the uterine cervix (apropos of 2 cases)]. 20154640220
effects of adaptation of in vitro rumen culture to garlic oil, nitrate, and saponin and their combinations on methanogenesis, fermentation, and abundances and diversity of microbial populations.this study investigated the effects of garlic oil (0.25 g/l), nitrate (5 mm), and quillaja saponin (0.6 g/l), alone and in binary or ternary combinations, on methanogenesis, rumen fermentation, and abundances of select microbial populations using in vitro rumen cultures. potential adaptation to these compounds was also examined by repeated transfers of the cultures on alternate days until day 18. all treatments except saponin alone significantly decreased methanogenesis. ternary combinations of ...201526733975
evaluating models of cellulose degradation by fibrobacter succinogenes s85.fibrobacter succinogenes s85 is an anaerobic non-cellulosome utilizing cellulolytic bacterium originally isolated from the cow rumen microbial community. efforts to elucidate its cellulolytic machinery have resulted in the proposal of numerous models which involve cell-surface attachment via a combination of cellulose-binding fibro-slime proteins and pili, the production of cellulolytic vesicles, and the entry of cellulose fibers into the periplasmic space. here, we used a combination of rna-seq ...201526629814
abundance of ruminal bacteria, epithelial gene expression, and systemic biomarkers of metabolism and inflammation are altered during the peripartal period in dairy cows.seven multiparous holstein cows with a ruminal fistula were used to investigate the changes in rumen microbiota, gene expression of the ruminal epithelium, and blood biomarkers of metabolism and inflammation during the transition period. samples of ruminal digesta, biopsies of ruminal epithelium, and blood were obtained during -14 through 28d in milk (dim). a total of 35 genes associated with metabolism, transport, inflammation, and signaling were evaluated by quantitative reverse transcription- ...201526409956
microbial populations and fermentation profiles in rumen liquid and solids of holstein cows respond differently to dietary barley processing.to evaluate the effects of treating barley grain with lactic acid (la) and heat on postprandial dynamics of 19 microbial taxa and fermentation in the rumen of dairy cows.201526399366
diversity of diazotrophic gut inhabitants of pikas (ochotonidae) revealed by pcr-dgge analysis.diazotrophic gut symbionts are considered to act as nitrogen providers for their hosts, as was shown for various termite species. although the diet of lagomorphs, like pikas or rabbits, is very poor in nitrogen and energy, their fecal matter contains 30-40% of protein. since our hypothesis was that pikas maintained a diazotrophic consortium in their gastrointestinal tract, we conducted the first investigation of microbial diversity in pika guts. we obtained gut samples from animals of several oc ...201525842897
biogas production from brewery spent grain enhanced by bioaugmentation with hydrolytic anaerobic bacteria.lignocellulosic substrates are widely available but not easily applied in biogas production due to their poor anaerobic degradation. the effect of bioaugmentation by anaerobic hydrolytic bacteria on biogas production was determined by the biochemical methane potential assay. microbial biomass from full scale upflow anaerobic sludge blanket reactor treating brewery wastewater was a source of active microorganisms and brewery spent grain a model lignocellulosic substrate. ruminococcus flavefaciens ...201525836034
effect of tannins and saponins in samanea saman on rumen environment, milk yield and milk composition in lactating dairy cows.the objective of this study was to investigate the effects of tannins and saponins in samanea saman on rumen fermentation, milk yield and milk composition in lactating dairy cows. four multiparous early-lactating dairy cows (holstein-friesian cross-bred, 75%) with an initial body weight (bw) of 405 ± 40 kg and 36 ± 8 day in milk were randomly assigned to receive dietary treatments according to a 4 × 4 latin square design. the four dietary treatments were unsupplemented (control), supplemented wi ...201524814291
temperature impacts differentially on the methanogenic food web of cellulose-supplemented peatland soil.the impact of temperature on the largely unresolved intermediary ecosystem metabolism and associated unknown microbiota that link cellulose degradation and methane production in soils of a moderately acidic (ph 4.5) fen was investigated. supplemental [(13) c]cellulose stimulated the accumulation of propionate, acetate and carbon dioxide as well as initial methane production in anoxic peat soil slurries at 15°c and 5°c. accumulation of organic acids at 15°c was twice as fast as that at 5°c. 16s r ...201524813682
monitoring of gene expression in fibrobacter succinogenes s85 under the co-culture with non-fibrolytic ruminal bacteria.fibrobacter succinogenes is one of the most pivotal fibrolytic bacterial species in the rumen. in a previous study, we confirmed enhancement of fiber digestion in a co-culture of f. succinogenes s85 with non-fibrolytic ruminal strains r-25 and/or selenomonas ruminantium s137. in the present study, mrna expression level of selected functional genes in the genome of f. succinogenes s85 was monitored by real-time rt-pcr. growth profile of f. succinogenes s85 was similar in both the monoculture and ...201525354721
a phylogenomic analysis of the bacterial phylum fibrobacteres.the fibrobacteres has been recognized as a bacterial phylum for over a decade, but little is known about the group beyond its environmental distribution, and characterization of its sole cultured representative genus, fibrobacter, after which the phylum was named. based on these incomplete data, it is thought that cellulose hydrolysis, anaerobic metabolism, and lack of motility are unifying features of the phylum. there are also contradicting views as to whether an uncultured sister lineage, can ...201526779135
effects of garlic oil, nitrate, saponin and their combinations supplemented to different substrates on in vitro fermentation, ruminal methanogenesis, and abundance and diversity of microbial populations.to investigate the effect of garlic oil (g), nitrate (n), saponin (s) and their combinations supplemented to different forage to concentrate substrates on methanogenesis, fermentation, diversity and abundances of bacteria and archaea in vitro.201525846054
effects of flavonoid-rich plant extracts on in vitro ruminal methanogenesis, microbial populations and fermentation characteristics.the objective of this study was to evaluate the in vitro effects of flavonoid-rich plant extracts (pe) on ruminal fermentation characteristics and methane emission by studying their effectiveness for methanogenesis in the rumen. a fistulated holstein cow was used as a donor of rumen fluid. the pe (punica granatum, betula schmidtii, ginkgo biloba, camellia japonica, and cudrania tricuspidata) known to have high concentrations of flavonoid were added to an in vitro fermentation incubated with rume ...201525656200
potential of tannin-rich plants for modulating ruminal microbes and ruminal fermentation in sheep.the objective of this work was to study nutritional strategies for decreasing methane production by ruminants fed tropical diets, combining in vitro and in vivo methods. the in vitro approach was used to evaluate the dose effect of condensed tannins (ct) contained in leaves of gliricidia sepium, leucaena leucocephala, and manihot esculenta (39, 75, and 92 g ct/kg dm, respectively) on methane production and ruminal fermentation characteristics. tannin-rich plants (trp) were incubated for 24 h alo ...201525568379
characterization of the cellulolytic bacteria communities along the gastrointestinal tract of chinese mongolian sheep by using pcr-dgge and real-time pcr analysis.a balanced gastrointestinal microbial ecosystem is crucial for the health and growth of animals. in the gastrointestinal tract (git) of ruminants, cellulolytic bacteria aid in the digestion and absorption of nutrients. rumen contents and feces in ruminants are often used to assess gastrointestinal microbial communities; however, these sites do not guarantee to represent the diversity of microbes found in the entire git. in this study, we investigated the microbiota along the git of five chinese ...201525931374
rapid changes in key ruminal microbial populations during the induction of and recovery from diet-induced milk fat depression in dairy cows.the ruminant provides a powerful model for understanding the temporal dynamics of gastrointestinal microbial communities. diet-induced milk fat depression (mfd) in the dairy cow is caused by rumen-derived bioactive fatty acids, and is commonly attributed to the changes in the microbial population. the aim of the present study was to determine the changes occurring in nine ruminal bacterial taxa with well-characterised functions, and abundance of total fungi, ciliate protozoa and bacteria during ...201526123320
effect of dietary supplementation with resveratrol on nutrient digestibility, methanogenesis and ruminal microbial flora in sheep.two experiments were conducted to evaluate the effect of resveratrol on methanogenesis and microbial flora in dorper × thin-tailed han cross-bred ewes. in experiment 1, ten ewes (67.2 ± 2.24 kg bw) were assigned to two dietary treatments, a basal diet and a basal diet supplemented with resveratrol (0.25 g/head·day), to investigate the effect of resveratrol on nutrient digestibility and nitrogen balance. in experiment 2, six ewes (64.0 ± 1.85 kg bw) with ruminal cannulae were assigned to the iden ...201525319536
effects of isobutyrate supplementation on ruminal microflora, rumen enzyme activities and methane emissions in simmental steers.the objective of this study was to evaluate the effects of isobutyrate supplementation on rumen microflora, enzyme activities and methane emissions in simmental steers consuming a corn stover-based diet. eight ruminally cannulated simmental steers were used in a replicated 4 × 4 latin square experiment. the treatments were control (without isobutyrate), low isobutyrate (lib), moderate isobutyrate (mib) and high isobutyrate (hib) with 8.4, 16.8 and 25.2 g isobutyrate per steer per day respectivel ...201524702602
[use of mycostatic drugs in dermatology]. 20154696265
[hospital mortality due to myocardial infarct]. 20154686681
[psychodiagnostic methods for early diagnosis of cerebrovascular insufficiency]. 20154668043
a policy perspective on the united states rehabilitation system. 20155042035
a policy perspective on the united states rehabilitation system. 20155042035
in vitro-in vivo correlation of drug metabolism--deamination of 1- -d-arabinofuranosylcytosine. 20154500983
[proceedings: effect of glutamate on retinal horizontal cells]. 20154478363
[primary noxious psychological influences in childhood]. 20154392294
[radiotherapy following synchronization and its theoretical prerequisites]. 20154341102
surgical treatment of organic hyperinsulinism with a report of 11 cases. 20154336330
effects of nitrate addition on rumen fermentation, bacterial biodiversity and abundance.this study examined changes of rumen fermentation, ruminal bacteria biodiversity and abundance caused by nitrate addition with ion torrent sequencing and real-time polymerase chain reaction. three rumen-fistulated steers were fed diets supplemented with 0%, 1%, and 2% nitrate (dry matter %) in succession. nitrate supplementation linearly increased total volatile fatty acids and acetate concentration obviously (p = 0.02; p = 0.02; p<0.01), butyrate and isovalerate concentration numerically (p = 0 ...201526194220
influence of substrates on the surface characteristics and membrane proteome of fibrobacter succinogenes s85.although fibrobacter succinogenes s85 is one of the most proficient cellulose degrading bacteria among all mesophilic organisms in the rumen of herbivores, the molecular mechanism behind cellulose degradation by this bacterium is not fully elucidated. previous studies have indicated that cell surface proteins might play a role in adhesion to and subsequent degradation of cellulose in this bacterium. it has also been suggested that cellulose degradation machinery on the surface may be selectively ...201526492413
effects of momordica charantia saponins on in vitro ruminal fermentation and microbial population.this study was conducted to investigate the effects of momordica charantia saponin (mcs) on ruminal fermentation of maize stover and abundance of selected microbial populations in vitro. five levels of mcs supplements (0, 0.01, 0.06, 0.30, 0.60 mg/ml) were tested. the ph, nh3-n, and volatile fatty acid were measured at 6, 24, 48 h of in vitro mixed incubation fluids, whilst the selected microbial populations were determined at 6 and 24 h. the high dose of mcs increased the initial fractional rat ...201626949950
isolation and characterization of a novel glycosyl hydrolase family 74 (gh74) cellulase from the black goat rumen metagenomic library.this study aimed to isolate and characterize a novel cellulolytic enzyme from black goat rumen by using a culture-independent approach. a metagenomic fosmid library was constructed from black goat rumen contents and screened for a novel cellulase. the kg37 gene encoding a protein of 858 amino acid residues (92.7 kda) was isolated. the deduced protein contained a glycosyl hydrolase family 74 (gh74) domain and showed 77% sequence identity to two endo-1,4-β-glucanases from fibrobacter succinogenes. ...201627866354
biochemical structural components of cloned n-nitrosomethylurea-induced astrocytoma cells grown subcutaneously. 20165061848
effect of hydroxyurea on the development of macronuclear anlagen in the exconjugants of blepharisma seshachari bhandary. 20165004155
[use of the indirect methemoglobin reduction test in the estimation of glucose-6-phosphate dehydrogenase in the blood of pregnant women]. 20164965012
[autopolymerizable acrylic molds for castings]. 20164949212
immunity to intracellular bacteria. 20164870271
phytochemical investigation of xanthium spinosum l. 20164853645
letter: the humanist physician. 20164847366
letter: drug-induced acanthosis nigricans. 20164847361
[radiotherapy in prostatic carcinoma]. 20164814513
[complications of surgery of the colon]. 20164782084
[case of objectively perceived tinnitus during palatal myoclonus]. 20164763015
speech defects in a national sample of children aged seven years. 20164759956
some x-ray units of interest. 20164731075
an unusual complication of pharyngolaryngooesophagectomy gastric transplant. 20164704652
effects of different sources of physically effective fiber on rumen microbial populations.physically effective fiber is needed by dairy cattle to prevent ruminal acidosis. this study aimed to examine the effects of different sources of physically effective fiber on the populations of fibrolytic bacteria and methanogens. five ruminally cannulated holstein cows were each fed five diets differing in physically effective fiber sources over 15 weeks (21 days/period) in a latin square design: (1) 44.1% corn silage, (2) 34.0% corn silage plus 11.5% alfalfa hay, (3) 34.0% corn silage plus 5. ...201626365790
uptake of milk with and without solid feed during the monogastric phase: effect on fibrolytic and methanogenic microorganisms in the gastrointestinal tract of calves.microbial communities are affected by diet and play a role in the successful transition from milk to a solid diet. the response of microorganisms in the gastrointestinal tract of holstein bull calves to the uptake of milk with solid feed (control treatment; ct), or milk without solid feed (milk-only treatment; mt) during the first 3 weeks of life was investigated. samples were collected from the rumen (fluid and tissue), abomasum (fluid), cecum (fluid and tissue) and feces at 7, 14 and 20 days o ...201626249227
synergism of cattle and bison inoculum on ruminal fermentation and select bacterial communities in an artificial rumen (rusitec) fed a barley straw based diet.this study evaluated the effect of increasing the proportion of bison relative to cattle inoculum on fermentation and microbial populations within an artificial rumen (rusitec). the experiment was a completely randomized design with a factorial treatment structure (proportion cattle:bison inoculum; 0:100, 33:67, 67:33, and 100:0) replicated in two rusitec apparatuses (n = 8 fermenters). the experiment was 15 d with 8 d of adaptation and 7 d of sampling. fermenters were fed a diet of 70:30 barley ...201628018336
effects of dietary supplementation of rumen-protected folic acid on rumen fermentation, degradability and excretion of urinary purine derivatives in growing steers.the present experiment was undertaken to determine the effects of dietary addition of rumen-protected folic acid (rpfa) on ruminal fermentation, nutrient degradability, enzyme activity and the relative quantity of ruminal cellulolytic bacteria in growing beef steers. eight rumen-cannulated jinnan beef steers averaging 2.5 years of age and 419 ± 1.9 kg body weight were used in a replicated 4 × 4 latin square design. the four treatments comprised supplementation levels of 0 (control), 70, 140 and ...201627666679
the in vitro effects of nano-encapsulated conjugated linoleic acid on stability of conjugated linoleic acid and fermentation profiles in the rumen.this study was aimed to evaluate the stability of conjugated linoleic acids (clas) by nano-encapsulation against in vitro ruminal biohydrogenation by microbial enzymatic conversion. clas (free fatty acid form of cla [cla-ffa], nano-encapsulated cla-ffa, triglyceride form of cla [cla-tg], and nano-encapsulated cla-tg) were used in the in vitro fermentation experiments. when butyrivibrio fibrisolvens (b. fibrisolvens) was incubated with cla-ffas, the concentrations of cis-9, trans-11 cla and vacce ...201626950867
live yeasts enhance fibre degradation in the cow rumen through an increase in plant substrate colonization by fibrolytic bacteria and fungi.to monitor the effect of a live yeast additive on feedstuff colonization by targeted fibrolytic micro-organisms and fibre degradation in the cow rumen.201626600313
ground transport stress affects bacteria in the rumen of beef cattle: a real-time pcr analysis.transport stress syndrome often appears in beef cattle during ground transportation, leading to changes in their capacity to digest food due to changes in rumen microbiota. the present study aimed to analyze bacteria before and after cattle transport. eight xianan beef cattle were transported over 1000 km. rumen fluid and blood were sampled before and after transport. real-time pcr was used to quantify rumen bacteria. cortisol and adrenocorticotrophic hormone (acth) were measured. cortisol and a ...201627696632
shifts in rumen fermentation and microbiota are associated with dissolved ruminal hydrogen concentrations in lactating dairy cows fed different types of carbohydrates.different carbohydrates ingested greatly influence rumen fermentation and microbiota and gaseous methane emissions. dissolved hydrogen concentration is related to rumen fermentation and methane production.201627511925
parameters of fermentation and rumen microbiota of nellore steers fed with different proportions of concentrate in fresh sugarcane containing diets.the objective of this study was to evaluate the effect of a fresh sugarcane-based diet and different roughage-to-concentrate ratios (70:30, 60:40, 40:60 and 20:80) on the rumen microbiota associated with rumen fermentation parameters and the intake and apparent digestibility of nutrients in nellore steers. eight rumen-cannulated nellore steers (331 ± 8 kg bw) were distributed in a double 4 × 4 latin square design balanced for the control of the residual effect. the ruminal ph decreased (p < 0.01 ...201627415825
dietary supplementation of rosmarinus officinalis l. leaves in sheep affects the abundance of rumen methanogens and other microbial populations.rumen microbiome has a great influence on ruminant health and productivity. different plant extracts have been tested for their ability to modulate the rumen microbiome to improve feed digestion and fermentation. among the evaluated plant extracts, essential oils, tannins, and saponins appeared to have positive effects on rumen protein metabolism, volatile fatty acids production, and methane and ammonia production.201627123239
rumen microbial abundance and fermentation profile during severe subacute ruminal acidosis and its modulation by plant derived alkaloids in vitro.rumen microbiota have important metabolic functions for the host animal. this study aimed at characterizing changes in rumen microbial abundances and fermentation profiles using a severe subacute ruminal acidosis (sara) in vitro model, and to evaluate a potential modulatory role of plant derived alkaloids (pda), containing quaternary benzophenanthridine and protopine alkaloids, of which sanguinarine and chelerythrine were the major bioactive compounds. induction of severe sara strongly affected ...201626868619
rumen adaptation of swamp buffaloes (bubalus bubalis) by high level of urea supplementation when fed on rice straw-based diet.four rumen-fistulated swamp buffaloes (bubalus bubalis) were randomly allocated to investigate rumen adaptation of urea on feed intake, nutrient digestibility, fermentation efficiency, and microbial protein synthesis. buffaloes were fed with rice straw ad libitum for a period of 2 weeks and then were shifted to a step-up diet regimen by supplementation of concentrate containing 20 and 40 g/kg urea at 5 g/kg bw for a period of 2 and 4 weeks, respectively. the results revealed that feed intake and ...201627107749
effect of dietary fiber on the methanogen community in the hindgut of lantang gilts.the primary objective of this study was to investigate the effect of dietary fiber on methanogenic diversity and community composition in the hindgut of indigenous chinese lantang gilts to explain the unexpected findings reported earlier that lantang gilts fed low-fiber diet (lfd) produced more methane than those fed high-fiber diet (hfd). in total, 12 lantang gilts (58.7±0.37 kg) were randomly divided into two dietary groups (six replicates (pigs) per group) and fed either lfd (ndf=201.46 g/kg) ...201627052363
changes in rumen microbial community composition during adaption to an in vitro system and the impact of different forages.this study examined ruminal microbial community composition alterations during initial adaption to and following incubation in a rumen simulation system (rusitec) using grass or corn silage as substrates. samples were collected from fermenter liquids at 0, 2, 4, 12, 24, and 48 h and from feed residues at 0, 24, and 48 h after initiation of incubation (period 1) and on day 13 (period 2). microbial dna was extracted and real-time qpcr was used to quantify differences in the abundance of protozoa, ...201626928330
modulatory effects of condensed tannin fractions of different molecular weights from a leucaena leucocephala hybrid on the bovine rumen bacterial community in vitro.condensed tannin (ct) fractions of different molecular weights (mws) may affect rumen microbial metabolism by altering bacterial diversity. in this study the effects of unfractionated cts (f0) and five ct fractions (f1-f5) of different mws (f1, 1265.8 da; f2, 1028.6 da; f3, 652.2 da; f4, 562.2 da; f5, 469.6 da) from leucaena leucocephala hybrid-rendang (llr) on the structure and diversity of the rumen bacterial community were investigated in vitro.201626910767
effects of corn silage and grass silage in ruminant rations on diurnal changes of microbial populations in the rumen of dairy cows.here, we examined diurnal changes in the ruminal microbial community and fermentation characteristics of dairy cows fed total mixed rations containing either corn silage (cs) or grass silage (gs) as forage. the rations, which consisted of 52% concentrate and 48% gs or cs, were offered for ad libitum intake over 20 days to three ruminal-fistulated lactating jersey cows during three consecutive feeding periods. feed intake, ruminal ph, concentrations of short chain fatty acids and ammonia in rumen ...201627451293
diet-dependent modular dynamic interactions of the equine cecal microbiota.knowledge on dynamic interactions in microbiota is pivotal for understanding the role of bacteria in the gut. we herein present comprehensive dynamic models of the horse cecal microbiota, which include short-chained fatty acids, carbohydrate metabolic networks, and taxonomy. dynamic models were derived from time-series data in a crossover experiment in which four cecum-cannulated horses were fed a starch-rich diet of hay supplemented with barley (starch intake 2 g kg(-1) body weight per day) and ...201627773914
comprehensive detection of bacterial carbohydrate-active enzyme coding genes expressed in cow rumen.to find the abundant and characteristic fibrolytic enzyme-coding gene expressed in fiber-associating microbiota, a metatranscriptomic data set was obtained from fiber-associating microbiota, and it was compared with that of rumen fluid-floating microbiota and two metagenomic data sets. fibrolytic rumen bacteria associate with plant polysaccharide and hydrolyze it in the rumen. we obtained a metatranscriptomic assembly from fiber-associating microbiota in three ruminally fistulated holstein cows ...201626875748
quantitative analysis of ruminal bacterial populations involved in lipid metabolism in dairy cows fed different vegetable oils.vegetable oils are used to increase energy density of dairy cow diets, although they can provoke changes in rumen bacteria populations and have repercussions on the biohydrogenation process. the aim of this study was to evaluate the effect of two sources of dietary lipids: soybean oil (so, an unsaturated source) and hydrogenated palm oil (hpo, a saturated source) on bacterial populations and the fatty acid profile of ruminal digesta. three non-lactating holstein cows fitted with ruminal cannulae ...201627146195
dominantly inherited digito-talar dysmorphism. 20165066209
[tumors and related processes of the reticuloendothelial system of the lymph nodes]. 20164954948
factors affecting cone-hysterectomy morbidity. a study of 200 patients. 20165088377
proceedings: increased faecal excretion of bile-acids in post-vagotomy diarrhoea. 20164752780
hazards to children in the home environment. 20164846661
model of the organization of dna in the chromosomes of higher organisms (phylo- and ontogenetic aspects). 20165005244
leishmaniasis in brazil. 8. observations on the phlebotomine fauna of an area highly endemic for cutaneous leishmaniasis, in the serra dos carajas, pará state. 20164784054
a serum substitute that can support the continuous growth of mammary tumor cells. 20164737673
genetic effects of radiation. 20164892645
[cleft lip and cleft palate. psychologial aspects. 8]. 20164771158
clinical and electroencephalographic effects of cinanserin in schizophrenic and manic patients. 20164929084
[in memoriam prof. behrend behrens, m.d., 23 may 1895--30 december 1969]. 20164923644
computation of transit time distributions using sampled data laplace transforms. 20164933900
identification of repeating units in dna concatemers. 20164852346
dietary supplementation with mulberry leaf flavonoids inhibits methanogenesis in sheep.the effects of flavonoids on methanogenesis and microbial flora in dorper × thin-tailed han crossbred ewes were evaluated in two experiments. to investigate the effects of flavonoids on nutrient digestibility and nitrogen balance, 18 ewes (60.0 ± 1.73 kg body weight (bw)) were allotted to two dietary treatments in experiment one, a control diet and the control diet supplemented with flavonoids (2 g/head/day). in experiment two, the effects of supplementary flavonoids on ruminal fermentation and ...201727112278
comparative fibre-degrading capacity in foals at immediate and late post-weaning periods.total tract apparent digestibility of dietary fibrous components and parameters of the faecal digestive ecosystem were compared at immediate and late post-weaning periods using five foals. the foals were abruptly weaned (day 0) from their mares at 6 months of age. immediately (day 0 to day 14) and later (day 131 to day 194) after weaning, foals received the same basal diet consisting of hay and pellets. pellets were fed at 0.36% bw. hay was offered ad libitum and corresponded to 120% of the aver ...201728219467
effects of the dose and viability of saccharomyces cerevisiae. 1. diversity of ruminal microbes as analyzed by illumina miseq sequencing and quantitative pcr.this study was conducted to examine effects of the dose and viability of supplemental saccharomyces cerevisiae on the ruminal fermentation and bacteria population and the performance of lactating dairy cows. four ruminally cannulated lactating cows averaging 284±18d in milk were assigned to 4 treatments arranged in a 4×4 latin square design with four 21-d periods. cows were fed a total mixed ration containing 41.7% corn silage, 12.1% brewer's grains, and 46.2% concentrate on a dry matter basis. ...201727837973
effect of sugarcane fiber digestibility, conservation method and concentrate level on the ruminal ecosystem of beef cattle.the aim of this study was to investigate the effects of sugarcane neutral detergent fiber digestibility (ndfd), conservation method, and concentrate level on the ruminal microbial population of steers. eight ruminal-cannulated nellore steers were distributed in two contemporary 4 × 4 latin square design with a 2 × 2 factorial arrangement of treatments. experiment 1: diets were formulated with 60% of concentrate level, and two sugarcane genotypes (high or low ndfd) either freshly cut or as silage ...201728265975
effects of addition of aspergillus oryzae culture and 2-hydroxyl-4-(methylthio) butanoic acid on milk performance and rumen fermentation of dairy cows.to investigate effects of aspergillus oryzae culture (aoc) and 2-hydroxy-4-(methylthio) butanoic acid (hmb) on milk performance and rumen fermentation of dairy cows. sixty-four multiparous chinese holstein cows were randomly allocated into four experimental diets: (i) control diet; (ii) aoc diet: 5 g aoc/day per head; (iii) hmb diet: 25 g hmb/day; and (iv) ah diet: 5 g aoc plus 25 g hmb/day. added hmb tended to increase the yield of milk protein (p = 0.06) and 3.5% fat-corrected milk (p = 0.08) ...201727506446
factors influencing ruminal bacterial community diversity and composition and microbial fibrolytic enzyme abundance in lactating dairy cows with a focus on the role of active dry yeast.the objective of the current study was to employ a dna-based sequencing technology to study the effect of active dry yeast (ady) supplementation, diet type, and sample location within the rumen on rumen bacterial community diversity and composition, and to use an rna-based method to study the effect of ady supplementation on rumen microbial metabolism during high-grain feeding (hg). our previous report demonstrated that the supplementation of lactating dairy cows with ady attenuated the effect o ...201728390722
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