Publications

nhaa, an na(+)/h(+) antiporter involved in environmental survival of vibrio cholerae.	vibrio cholerae, the agent of cholera, is a normal inhabitant of aquatic environments, in which it survives under a wide range of conditions of ph and salinity. in this work, we identified the nhaa gene in a wild-type epidemic strain of v. cholerae o1. nhaa encodes a protein of 382 amino acids that is very similar to the proteins nhaa of vibrio parahaemolyticus, vibrio alginolyticus ( approximately 87% identity), and escherichia coli (56% identity). v. cholerae nhaa complements an e. coli nhaa m ...	2000	10781565
distribution of vibrio cholerae virulence genes among different vibrio species isolated in sardinia, italy.	the members of the genus vibrio include harmless aquatic strains as well as strains capable of causing epidemics of cholera. diarrhoea caused by vibrio cholerae is attributed to cholerae enterotoxin (ct) codified by the ctx operon and regulated by a number of virulence genes such as toxt, toxr and toxs. fifty-two vibrio strains were isolated from different aquatic environments in and around sardinia and searched by pcr for the presence of ctxa, zot, ace, toxr, toxs, toxt, tcpa and vpi virulence ...	2000	10747228
virulence factors in vibrio alginolyticus strains isolated from aquatic environments.	in a microbiological monitoring carried out in various aquatic environment of sardinia island (italy) vibrio alginolyticus with different virulence phenotypes appeared widely spread. hemolysis, hemoagglutination and protease production might be together particularly in strains isolated from polluted environments. adherence capacities to two epithelial cells (hep-2 and caco-2) available in laboratory were widely spread in the examined bacterial strains. the adhesion degree was influenced by the u ...	2000	11235505
metal content and biochemical analyses of a recombinant collagenase prtv from vibrio parahaemolyticus.	prtv is an extracellular metalloprotease of vibrio parahaemolyticus and regarded as a collagenase. inductively coupled plasma-optical emission spectrometry analysis indicated that the recombinant prtv contains 1 mol of zinc per mol of the native enzyme. on the basis of a kinetic study using 2-furanacryloyl-leu-gly-pro-ala (falgpa, the specific substrate for bacterial collagenase) as a substrate, it was suggested that metal ions may play a significant role in the binding and catalytic steps of th ...	2000	11128063
lethal attribute of serine protease secreted by vibrio alginolyticus strains in kuruma prawn penaeus japonicus.	toxicity of the extracellular products (ecp) and the lethal attribute of serine protease secreted by five pathogenic vibrio alginolyticus strains from various sources in kuruma prawn penaeus japonicus were studied. the ecps of organisms originally isolated from diseased kuruma prawn or small abalone haliotis diversicolor supertexta were more lethal (ld50 value of 0.48 or 0.41 microg protein/g prawn) than those from diseased tiger prawn p. monodon, yellowfin porgy acanthopagrus latus or horse mac ...	2000	10739107
detection of the na(+)-translocating nadh-quinone reductase in marine bacteria using a pcr technique.	to examine the distribution of the na(+)-translocating nadh-quinone reductase (na(+)-nqr) among marine bacteria, we developed a simple screening method for the detection of this enzyme. by reference to the homologous sequences of the na(+)-nqr operons from vibrio alginolyticus and haemophilus influenzae, a pair of primers was designed for amplification of a part of the sixth orf (nqr6) of the na(+)-nqr operon. when pcr was performed using genomic dna from 13 marine bacteria, a 0.9-kbp fragment c ...	2000	10779868
characterization of a flagellar sheath component, pf60, and its structural gene in marine vibrio.	the polar flagella (pof) of vibrio alginolyticus are surrounded by a membrane structure called a sheath. five major proteins, whose molecular masses are 60, 47, 45, 44, and 18 kda (named pf60, pf47, pf45, pf44, and pf18, respectively), have been detected in polar flagella. pf47 and pf45 have been identified as flagellins while the other proteins are thought to be sheath-associated ones. in this study, we isolated and partially characterized a major sheath protein, pf60. we found that pf60 can be ...	2000	10731663
functional reconstitution of the na(+)-driven polar flagellar motor component of vibrio alginolyticus.	the bacterial flagellar motor is a molecular machine that couples the influx of specific ions to the generation of the force necessary to drive rotation of the flagellar filament. four integral membrane proteins, poma, pomb, motx, and moty, have been suggested to be directly involved in torque generation of the na(+)-driven polar flagellar motor of vibrio alginolyticus. in the present study, we report the isolation of the functional component of the torque-generating unit. the purified protein c ...	2000	10681557
[bioenergetics of marine bacteria--respiration-coupled na+ pump].	marine bacteria are unique in the requirement for na+ for optimal growth. using a marine bacterium vibrio alginolyticus, it was confirmed that na+ is essential for the active uptake of all amino acids. furthermore, the respiratory chain of v. alginolyticus was found to require na+ for the maximum activity. the site of na(+)-dependent activation is localized in the nadh-quinone reductase segment, where na+ is extruded from the cells as a direct result of redox reaction. thus, marine bacteria are ...	2000	10655779
removal of a high load of ammonia gas by a marine bacterium, vibrio alginolyticus.	a newly isolated marine bacterium, vibrio alginolyticus was used to remove a high load of ammonia gas. by a stepwise increase in ammonia supply over the concentration range of 120-2000 ppm (v/v), complete removal of ammonia was observed from the start of the experiment in a suspended culture of the bacterium in basal medium containing 3% nacl. when cells were inoculated onto an inorganic packing material in a biofilter, and a high load of ammonia was introduced continuously under nonsterile cond ...	2000	16232881
cloning and sequencing of a chitinase gene from vibrio alginolyticus h-8.	a gene from vibrio alginolyticus h-8, encoding chitinase, designated as chitinase b, was cloned by the shot-gun method using puc118 and sequenced. the open reading frame consisted of 846 amino acids including a signal peptide. the molecular mass of the enzyme estimated based on the amino acid sequence data was 90 kda. the n-terminal amino acid sequence of the enzyme was different from that of chitinase c1 which we had previously reported. this cloned chitinase b was considered one out of four ch ...	2000	16232787
cloning and sequencing of the deacetylase gene from vibrio alginolyticus h-8.	a gene encoding deacetylase da1 that is specific for n, n'-diacetylchitobiose was cloned using the shot-gun method with puc118 and sequenced. the open reading frame encoded a protein of 427 amino acids including the signal peptide. the molecular mass of the mature enzyme estimated from the amino acid sequence data was 44.7 kda, which is approximately similar to that, estimated by sds-page (48.0 kda), of the purified enzyme reported previously. the n-terminal amino acid sequence deduced from the ...	2000	16232910
multimeric structure of poma, a component of the na+-driven polar flagellar motor of vibrio alginolyticus.	four integral membrane proteins, poma, pomb, motx, and moty, are thought to be directly involved in torque generation of the na(+)-driven polar flagellar motor of vibrio alginolyticus. our previous study showed that poma and pomb form a complex, which catalyzes sodium influx in response to a potassium diffusion potential. poma forms a stable dimer when expressed in a pomb null mutant. to explore the possible functional dependence of poma domains in adjacent subunits, we prepared a series of poma ...	2000	10783392
a slow-motility phenotype caused by substitutions at residue asp31 in the poma channel component of a sodium-driven flagellar motor.	poma is thought to be a component of the ion channel in the sodium-driven polar-flagellar motor of vibrio alginolyticus. we have found that some cysteine substitutions in the periplasmic region of poma result in a slow-motility phenotype, in which swarming and swimming speeds are reduced even in the presence of high concentrations of nacl. most of the mutants showed a sodium ion dependence similar to that of the wild type but with significantly reduced motility at all sodium ion concentrations. ...	2000	10809720
covalently bound flavin in the nqrb and nqrc subunits of na(+)-translocating nadh-quinone reductase from vibrio alginolyticus.	na(+)-translocating nadh-quinone reductase (nqr) from the marine bacterium vibrio alginolyticus is composed of six subunits (nqra to nqrf). on sds-page of the purified complex, nqrb and nqrc subunits were found to give yellow-green fluorescent bands under uv illumination. both the nqrb and nqrc, electroeluted from the gel, had an absorption maximum at 448 nm, and the fluorescence excitation maxima at 365 and 448 nm and the emission maximum at 514 nm. the electroeluted nqrb and nqrc, respectively ...	2000	10838078
an unusual source of vibrio alginolyticus-associated otitis: prolonged colonization or freshwater exposure?	otitis externa and otitis media are commonly encountered in clinical practice. we report an unusual case of otitis externa, which was caused by vibrio alginolyticus, several months after saltwater exposure. clinicians need to be aware of this unusual pathogen, especially in refractory cases of ear infections.	2000	10864119
coupling ion specificity of chimeras between h(+)- and na(+)-driven motor proteins, motb and pomb, in vibrio polar flagella.	we have shown that a hybrid motor consisting of proton-type rhodobacter sphaeroides mota and sodium-type vibrio: alginolyticus pomb, motx and moty, can work as a sodium-driven motor in vibrio: cells. in this study, we tried to substitute the b subunits, which contain a putative ion-binding site in the transmembrane region. rhodobacter sphaeroides motb did not work with either mota or poma in vibrio cells. therefore, we constructed chimeric proteins (momb), which had n-terminal motb and c-termina ...	2000	10899118
intermolecular cross-linking between the periplasmic loop3-4 regions of poma, a component of the na+-driven flagellar motor of vibrio alginolyticus.	poma and pomb form a complex that conducts sodium ions and generates the torque for the na(+)-driven polar flagellar motor of vibrio alginolyticus. poma has four transmembrane segments. one periplasmic loop (loop(1-2)) connects segments 1 and 2, and another (loop(3-4)), in which cysteine-scanning mutagenesis had been carried out, connects segments 3 and 4. when poma with an introduced cys residue (cys-poma) in the c-terminal periplasmic loop (loop(3-4)) was examined without exposure to a reducin ...	2000	10900193
hepatopancreatic alterations in litopenaeus vannamei (boone, 1939) (crustacea: decapoda: penaeidae) experimentally infected with a vibrio alginolyticus strain.	as in other countries, the presence of disease has affected and limited the development of shrimp culture in venezuela. vibriosis is one of the most prevalent, causing high mortality not only in larval cultures but also in shrimp production. the present work shows the histological changes in hepatopancreas of litopenaeus vannamei experimentally induced by a strain of vibrio alginolyticus. the shrimps were infected by the immersion technique, being exposed to a concentration of 5.2 x 10(7) bacter ...	2000	10963396
epidermal lesions and mortality caused by vibriosis in deep-sea bahamian echinoids: a laboratory study.	when significant mortality of the bathyal spatangoid echinoid paleopneustes cristatus occurred under laboratory conditions, we investigated the cause and course of the disease by culturing and identifying internal pathogens, then experimentally infecting healthy urchins with isolates of the suspected disease organism. the pathogen was determined to be the gram-negative halophilic bacterium vibrio alginolyticus. this species was also recovered from frozen post-challenge specimens of p. cristatus ...	2000	10768287
isolation of vibrio alginolyticus from seawater aquaria.	the seawater bacterium vibrio alginolyticus was detected in 5 of 20 water samples from seawater aquaria (from 3 of 5 units) and also from the surface of diseased stony corals. a total of 45 isolates were differentiated biochemically, of which 13 isolates (29%) proved to be v. alginolyticus. all those strains produced the virulence factors caseinase and lipase, 11 strains amylase and gelatinase. 7 strains showed lecithinase activity and 2 strains produced hemolysins. all examined strains showed a ...	2000	11109571
frontiers of quantitative cytochemistry: a review of recent developments and potentials.	this article attempts to describe the current state of quantitative cytochemical methods and to point out areas where, in the author's opinion, the greatest progress is either being made or can be expected. nearly 300 references to the current literature are cited.	2000	94776
[electro- and phonocardiogram indices in newborn infants with hemolytic disease].		2000	101960
[adjuvant and palliative treatments for colo-rectal cancer].		2000	94441
mesonephric duct remnants (gartner's duct).	in a review of 820 hysterosalpingograms, unilateral opacification of the mesonephric duct (gartner's duct) was observed in five individuals. no associated anomalies of the genitourinary tract or definite evidence of pelvic inflammatory disease was found in any of these patients. the embryology of the genitourinary tract is discussed in some detail, and histologic studies of the uterine cervix are reviewed to explain the radiographic observations.	2000	98997
interaction of alcohol and reward in an achievement situation.	effects of psychological stress on acute alcohol intoxication following intake of 2 ml whisky/kg body weight were examined in 12 healthy subjects. each subject performed a 45-min psychomotor task under four conditions, two of which increased achievement stress by promising the subject a monetary reward for rapid and accurate performance. the conditions were (a) task only, (b) task + reward, (c) task + alcohol, and (d) task + reward + alcohol. in condition (b) reward improved performance and heig ...	2000	111286
steroids in asthma.		2000	91910
drug-induced lung disease.	since there are no diagnostic studies to confirm the presence of a drug-induced lung reaction the physician will make a correct diagnosis only if he is aware of the drugs which have been identified to cause pulmonary reactions and their specific manifestations. failure to recognize a drug-induced lung disease can lead to significant morbidity and in some cases mortality. the major drug-induced lung diseases are reviewed, the drugs being presented in the context of their clinical use and the reac ...	2000	102005
can the family physician avoid conflict of interest in the gatekeeper role? an affirmative view.		2000	2723596
letter: reflex sympathetic dystrophy syndrome.		2000	59068
clinical trial of topical disodium chromoglycate in vernal keratoconjunctivitis.	the clinical picture of spring catarrh was studied in 96 cases. the duration of the disease was found to be 3.5 +/- 2.6 years. it exists all the year round with a higher frequency between april and august. male children were affected about three times more frequently than females. the effect of dscg was studied in 20 cases. it had a significant therapeutic effect. we advise a combined dscg and corticosteroid treatment in the period of exacerbation.	2000	101923
lack of uniformity in colour matching.	1. the fraction of red in a red--green mixture matched to yellow increased as the intensities of the match constituents were increased sufficiently to bleach appreciable chlorolabe and erythrolabe. 2. all changes in matching found for a given normal trichromat, (i) with increase in the intensities of the matching components, (ii) as a function of time after the onset of very intense components, (iii) with change in the pupil region through which light enters the eye, and (iv) with change in the ...	2000	313984
[diverticula of the duodenal orifice].	the authors present a synthesis of data from the literature, as well as of particularities that have confronted them in connection with 16 observations of mucous diverticuli of duodenal windows (of which 7 were interposed and 9 were juxtaposed). after a definition of the window diverticuli, of their types and of their repercussions, clinical data are commented upon, the possibilities offered by paraclinical investigations and by the intra-operatory methods, as well as the different therapeutical ...	2000	111314
[clinical application of beta 2-microglobulin measurement in malignant disease (author's transl)].		2000	92044
the rubella immunity gap.		2001	87852
transmission of hepatitis b by immune serum globulin.		2001	93197
antimalaria studies on qinghaosu.		2001	117984
preparation of liposomes of defined size distribution by extrusion through polycarbonate membranes.	liposomes of defined size and homogeneity have been prepared by sequential extrusion of the usual multilamellar vesicles through polycarbonate membranes. the process is easy, reproducible, produces no detectable degradation of the phospholipids, and can double the encapsulation efficiency of the liposome preparation. multilamellar vesicles extruded by this technique are shown by both negative stain and freeze-fracture electron microscopy to have mean diameters approaching the pore diameter of th ...	2001	95096
quality assurance of gram-stained direct smears.	clinical laboratories do not always prepare and examine direct smears. in part, this may be explained by lack of control over the precision and accuracy of the procedure, which has diminished confidence in direct smears among both laboratory scientists and clinicians. objective criteria were established for enumeration of cells and placement of bacteria in identification categories. two methods for monitoring the accuracy and reproducibility of their application were studied: (1) examination of ...	2001	92891
[combination radio and chemotherapy in plasmocytoma. roentgen diagnostic and protein chemistry development prognosis].		2001	95496
affinity sensors for individual metabolites.	specific metabolite sensors may be constructed for species when a specific binding agent and optically detectable competing assay ligands are available.	2001	94999
severe skin pain after puva treatment.	severe skin pain lasting one or two months occurred in 8 of 210 patients treated with puva. the pain started 4--8 weeks after the initial dose, mostly about one week after discontinuation of the treatment. it was a prickling, burning pain, usually coming in bouts and confined to limited areas "deep under the skin". in some respects the pain was related to itching, but the patients could easily distinguish between the two sensations. a variety of drugs was tried, but none had any noteworthy effec ...	2001	93376
[changes in physicochemical parameters and coagulation factors in hemodilution with dextran of molecular weight 60,000].	in 18 subjects undergoing aorto-bifemoral by-pass, pre-operative isovalaemic hemodilution was carried out (using a dextran of molecular weight 60,000) at the same time as venous section of a mean value of 1087 ml reducing the hematocrit to 0.30. the following were studied before, immediately after and 24 hours after hemodilution: blood viscosity (at variable shearing rates), oncotic pressure, sedimentation rate, clotting factors, plasma electrolytes. these results showed that at the end of hemod ...	2001	95471
[psychological diagnosis problems in children between 0 and 3 years of age].		2001	95256
determination of hbcag and anti-hbc by immune adherence hemagglutination and association of hbcag with e antigen.		2001	61024
isotachophoresis of csf proteins in gel tubes especially gammaglobulins. an analytical and preparative technique for high-separation of csf proteins.	an isotachophoretic method using polyacrylamide gel (pag-itp) in a simple disc electrophoretic equipment with plastic tubes containing the gels, was elaborated and especially designed for studying the gammaglobulins in csf and serum from control subjects and patients with neurological disorders, especially known or probable ms. the device and the itp system used, including leading and terminating electrolytes and spacer substances, dividing the gammaglobulins in a reproducible way, are described ...	2001	92548
perinatal mortality.		2001	93220
determination of small differences in buoyant density of macromolecules by analytical density gradient equilibrium centrifugation.	a sensitive method is proposed for the determination of small differences between the buoyant densities of different species of monodisperse macromolecules by analytical density gradient equilibrium centrifugation. the procedure involves the measurement at sedimentation equilibrium of the bandwidths of the concentration distribution of the separate macromolecules and of a mixture of the different species. the difference in buoyant densities can then be estimated from the difference between the b ...	2001	95253
long-term outpatient vasodilator therapy of congestive heart failure. consideration of agents at rest and during exercise.	increased left ventricular filling pressure and reduced cardiac output are two major hemodynamic deficits in pump failure. in patients with chronic heart failure, consequences of these hemodynamic deficits and diminished cardiac reserve are manifested initially during stress and eventually at rest. the purpose of therapeutic interventions include reduction of ventricular filling pressure increase in cardiac output and improvement in cardiac reserve. to achieve these goals, the hemodynamic effect ...	2001	99031
[current status of kidney transplantation and results].		2001	2860545
cloning and characterization of motx, a vibrio alginolyticus sodium-driven flagellar motor gene.	four motor proteins, motx, moty, poma, and pomb, have been identified as constituents of the na(+)-driven flagellum of vibrio species. in this study, the complete motx gene was cloned from vibrio alginolyticus and shown to complement three mot mutations, motx94, motx115, and motx119, as well as a v. parahaemolyticus motx mutant. the motx94 mutant contains a frameshift at val86 of motx, while the motx115 and motx119 mutations comprise substitutions of ala146 to val and gln 194 to amber, respectiv ...	2001	11726290
chemical defense in the egg masses of benthic invertebrates: an assessment of antibacterial activity in 39 mollusks and 4 polychaetes.	many marine invertebrates deposit benthic egg masses that are potentially vulnerable to microbial infection. to help counter this threat these species may have evolved some form of chemical protection for their encapsulated embryos. in this study the egg masses from 7 marine mollusks were tested for antibacterial activity against 4 marine pathogens: enterococcus sericolicida, vibrio anguillarum, vibrio alginolyticus, and vibrio harveyi. extracts from all of these egg masses were found to inhibit ...	2001	11812113
identification of the abc protein sapd as the subunit that confers atp dependence to the k+-uptake systems trk(h) and trk(g) from escherichia coli k-12.	the activity of the two almost identical k+-uptake systems, trk(h) and trk(g), from escherichia coli k-12 depends completely and partially on the presence of the trke gene, respectively. trke maps inside the sapabcdf operon, which encodes an atp-binding cassette (abc) transporter of unknown function from the subgroup of peptide-uptake systems. this study was carried out to clarify the role of sapabcdf gene products in the atp dependence of the e. coli trk systems. for this purpose deltasapabcdf ...	2001	11700350
pathogenicity of vibrio alginolyticus isolated from diseased small abalone haliotis diversicolor supertexta.	outbreaks of mass mortality among cultured small abalone haliotis diversicolor supertexta with abscess/ulcers in the mantle occurred in 1998 at kao-hsiung, taiwan. a swarming bacterium, strain h-11 was isolated from the haemolymph of the moribund small abalone using tryptic soy agar supplemented with 3% nacl and/or thiosulphate citrate bile salt sucrose agar. this strain was characterized and identified as vibrio alginolyticus on the basis of various biochemical tests. the h-11 strain and its ex ...	2001	11297013
fmn is covalently attached to a threonine residue in the nqrb and nqrc subunits of na(+)-translocating nadh-quinone reductase from vibrio alginolyticus.	the na(+)-translocating nadh-quinone reductase (nqr) from vibrio alginolyticus is composed of six subunits (nqra to nqrf). we previously demonstrated that both nqrb and nqrc subunits contain a flavin cofactor covalently attached to a threonine residue. fluorescent peptide fragments derived from the nqrb and nqrc subunits were applied to a matrix-assisted laser desorption ionization time-of-flight mass spectrometer, and covalently attached flavin was identified as fmn in both subunits. from post- ...	2001	11163785
recent progress in the na(+)-translocating nadh-quinone reductase from the marine vibrio alginolyticus.	the respiratory chain of gram-negative marine and halophilic bacteria has a na(+)-dependent nadh-quinone reductase that functions as a primary na(+) pump. the na(+)-translocating nadh-quinone reductase (nqr) from the marine vibrio alginolyticus is composed of six structural genes (nqra to nqrf). the nqrf subunit has non-covalently bound fad. there are conflicting results on the existence of other flavin cofactors. recent studies revealed that the nqrb and nqrc subunits have a covalently bound fl ...	2001	11248187
na(+) translocation by bacterial nadh:quinone oxidoreductases: an extension to the complex-i family of primary redox pumps.	the current knowledge on the na(+)-translocating nadh:ubiquinone oxidoreductase of the na(+)-nqr type from vibrio alginolyticus, and on na(+) transport by the electrogenic nadh:q oxidoreductases from escherichia coli and klebsiella pneumoniae (complex i, or ndh-i) is summarized. a general mode of redox-linked na(+) transport by nadh:q oxidoreductases is proposed that is based on the electrostatic attraction of a positively charged na(+) towards a negatively charged, enzyme-bound ubisemiquinone a ...	2001	11248188
difference between forward and backward swimming speeds of the single polar-flagellated bacterium, vibrio alginolyticus.	the forward and backward swimming speeds and periods of a vibrio alginolyticus strain that has a single polar flagellum were measured. the backward swimming speeds were 1.5 times greater than the forward ones on average and the average period of backward swimming was shorter than forward swimming. however, the swimming speed and period were not correlated. similar results were obtained for a mutant that has a 1.6 times longer flagellum on average.	2001	11750825
vibrio alginolyticus acute gastroenteritis: report of two cases.		2001	11298156
in vitro susceptibility of vibrio spp. isolated from the environment.	bacteria of the genus vibrio include harmless aquatic strains as well as strains capable of causing epidemics of cholera and human intestinal diseases. some of these species may show resistance to different antibiotics including cefotaxime, tetracycline and chloramphenicol. the susceptibility to different antibiotics was tested using 40 vibrio alginolyticus, eight v. parahaemolyticus and six v. vulnificus strains isolated in the coastal waters of northern sardinia (italy). the frequency of resis ...	2001	11337229
structures of ribonuclease p rnas of vibrio core species.	the structures of an rna component of ribonuclease p (rnase p rna) were examined for vibrio parahaemolyticus, vibrio alginolyticus, vibrio carchariae, vibrio natriegens, vibrio campbellii, vibrio proteolyticus, vibrio pelagius and vibrio harveyi to clearly determine their genetic differences. the rnase p rnas ranged from 382 to 454 nucleotides (nt) in size, and were remarkably different from each other in the structure of two helices, p3 and p12. the p3 helices were comprised of tandem repeats o ...	2001	11430405
residue aspartate-147 from the third transmembrane region of na(+)/h(+) antiporter nhab of vibrio alginolyticus plays a role in its activity.	nhab is a bacterial na(+)/h(+) antiporter with unique topology. the ph dependence of nhab from vibrio alginolyticus differs from that of the escherichia coli nhab homolog. replacement of asp-147 with glu made high h(+) concentrations a requirement for the nhab activity. replacement of asp-147 with neutral amino acids inactivated nhab.	2001	11544242
identification of bacteria crucial to histamine accumulation in pacific mackerel during storage.	bacterial growth and histamine formation in pacific mackerel during storage at 0, 4, 15, and 25 degrees c were monitored. to identify bacterial species contributing to histamine formation, several groups of bacteria were isolated by using selective media under temperatures corresponding to the various storage conditions. initially, low counts of bacteria were found in the gill, skin, and intestine of fresh fish, and only weak histamine formers were found in the gill. histamine was found in the m ...	2001	11601705
[immunotropic properties of exoglycan obtained from the marine micro-organism vibrio alginolyticus].	immune system modulating activity of exoglycane isolated from culture media of vibrio alginolyticus (strain 945-80) was investigated. the substance demonstrated stimulating activity on the humoral and cell immune system, potentiated phagocyte activity of macrophage and neutrophils, increased survival index of the animals infected by gram-positive and gram-negative bacteria.	2002	12077940
inactivation of the na+-translocating nadh:ubiquinone oxidoreductase from vibrio alginolyticus by reactive oxygen species.	the na+-translocating nadh:quinone oxidoreductase (na+-nqr) from vibrio alginolyticus was inactivated by reactive oxygen species. highest na+-nqr activity was observed in anaerobically prepared membranes that exhibited 1:1 coupling of nadh oxidation and q reduction activities (1.6 u x mg(-1)). optical and epr spectroscopy documented the presence of b-type cytochromes, a [2fe-2s] cluster and an organic radical signal in anaerobically prepared membranes from v. alginolyticus. it is shown that the ...	2002	11856363
cloning and sequence analysis of a novel metalloprotease gene from vibrio parahaemolyticus 04.	the metalloprotease gene (vppc) from vibrio parahaemolyticus 04 has been cloned and sequenced. the vppc gene contains an open reading frame of 2442 nucleotides encoding a polypeptide of 814 amino acids with a calculated molecular mass of 89,833 da. the predicted amino acid sequence of vppc containing a zinc metalloprotease hexxh consensus motif displays extensive homology to the collagenase from vibrio alginolyticus. the activity of the recombinant protease produced in escherichia coli was exami ...	2002	11867235
novel substituted quinoxaline 1,4-dioxides with in vitro antimycobacterial and anticandida activity.	thirty-six 6(7)-substituted-3-methyl- or 3-halogenomethyl-2-phenylthio-phenylsulphonyl-chloro-quinoxaline 1,4-dioxides belonging to series 3-6 were synthesised and submitted to a preliminary in vitro evaluation for antimycobacterial, anticandida and antibacterial activities. antitubercular screening showed a generally good activity of 3-methyl-2-phenylthioquinoxaline 1,4-dioxides (3d,e,h-j) against mycobacterium tuberculosis, and exhibited mic between 0.39 and 0.78 microg ml(-1) (rifampicin mic= ...	2002	12008050
usefulness of r72h pcr assay for differentiation between vibrio parahaemolyticus and vibrio alginolyticus species: validation by dna-dna hybridization.	we compared the efficiencies of biochemical methods and polymerase chain reaction (pcr) for the identification of vibrio parahaemolyticus strains. the 122 isolates studied, identified by biochemical tests as v. parahaemolyticus or vibrio alginolyticus, were tested by r72h pcr assay. the results obtained with the two methods were consistent for 90% of the strains studied. pcr amplification of the r72h fragment generated two unique amplicons, 387 bp and 320 bp in length. for 11% of the strains fro ...	2002	12393193
phylogenetic study and identification of human pathogenic vibrio species based on partial hsp60 gene sequences.	the use of hsp60 gene sequences for phylogenetic study and identification of pathogenic marine vibrios was investigated. a 600-bp partial hsp60 gene was amplified by pcr and sequenced from 29 strains representing 15 vibrio species within the family vibrionaceae. sequence comparison of the amplified partial hsp60 gene revealed 71-82% sequence identity among different vibrio species and 96-100% sequence identity among epidemiologically distinct strains with the same species designation. this degre ...	2002	12489780
korormicin insensitivity in vibrio alginolyticus is correlated with a single point mutation of gly-140 in the nqrb subunit of the na(+)-translocating nadh-quinone reductase.	na(+)-translocating nadh-quinone reductase (nqr) from the marine vibrio alginolyticus is strongly inhibited by a new antibiotic korormicin. korormicin specifically inhibits the na(+)-dependent reaction of the nqr complex and acts as a purely non-competitive inhibitor for q-1 with the inhibitor constant of 82 pm. korormicin-resistant mutants were isolated from v. alginolyticus and the nqr complex was purified from a mutant kr2. similar to 2-n-heptyl-4-hydroxyquinoline n-oxide (hqno), korormicin a ...	2002	12054467
presence of vibrios in seawater and mytilus galloprovincialis (lam.) from the mar piccolo of taranto (ionian sea).	during the spring-summer period, vibrios were detected in water and mussels (mytilus galloprovincialis) collected in 30 sampling sites located in the mar piccolo of taranto (ionian sea, italy). in order to evaluate the degree of microbial pollution of the investigated area, fecal coliforms and escherichia coli densities were also determined. vibrio alginolyticus constituted the predominant component of the total culturable vibrios. some vibrio species such as v. mediterranei, v. parahaemolyticus ...	2002	12369519
the systematic substitutions around the conserved charged residues of the cytoplasmic loop of na+-driven flagellar motor component poma.	poma, a homolog of mota in the h+-driven flagellar motor, is an essential component for torque generation in the na+-driven flagellar motor. previous studies suggested that two charged residues, r90 and e98, which are in the single cytoplasmic loop of mota, are directly involved in this process. these residues are conserved in poma of vibrio alginolyticus as r88 and e96, respectively. to explore the role of these charged residues in the na+-driven motor, we replaced them with other amino acids. ...	2002	12079395
comparative susceptibility of veliger larvae of four bivalve mollusks to a vibrio alginolyticus strain.	the susceptibility of 7 d old veliger larvae of the scallops argopecten ventricosus and nodipecten subnodosus, the penshell atrina maura, and the pacific oyster crassostrea gigas to a pathogenic strain of vibrio alginolyticus was investigated by challenging the larvae with different bacterial concentrations in a semi-static assay. the results indicate that the larvae of the 2 scallop species are more susceptible to the v. alginolyticus strain than those of the oyster and the penshell. signs of t ...	2002	12113308
motx and moty, specific components of the sodium-driven flagellar motor, colocalize to the outer membrane in vibrio alginolyticus.	rotation of the sodium-driven polar flagella of vibrio alginolyticus requires four motor proteins: poma, pomb, motx and moty. motx and moty, which are unique components of the sodium-driven motor of vibrio, have been believed to be localized in the inner (cytoplasmic) membrane via their n-terminal hydrophobic segments. here we show that motx and moty colocalize to the outer membrane. both proteins, when expressed together, were detected in the outer membrane fraction separated by sucrose density ...	2002	12366836
ilizarov technique in the treatment of chronic osteomyelitis caused by vibrio alginolyticus.	we present a case of 26-year-old woman with posttraumatic chronic osteomyelitis caused by vibrio alginolyticus, contracted after contamination of a tibial fracture with seawater. the patient underwent bone resection and bifocal osteosynthesis according to ilizarov and was treated with a combination of ciprofloxacin and tetracycline. the patient responded in the same way to distraction osteogenesis as any other patient with chronic osteomyelitis with large defects.	2002	12035144
vibrio alginolyticus as the cause of pleural empyema and bacteremia in an immunocompromised patient.		2002	12072928
comments on references to the american board of psychiatry and neurology in an article.		2002	3369584
[peculiarities in the development of 2 cases of acute otomastoiditis].		2002	135309
[multiple liver abscesses with spontaneous perforation into the free abdominal cavity in biliary tract obstruction. demonstration using percutaneous transhepatic cholangiography].		2002	122965
atpase activity and light scattering of acto-heavy meromyosin: dependence on atp concentration and on ionic strength.	1. the dependence on atp concentration of atpase activity and light scattering decrease of acto-hmm could be described at very low ionic strength by one hyperbolic adsorption isotherm with a dissociation constant of 3 x 10(-6)m. hence the increase of atp ase activity was paralleled by a decrease in light scattering. at higher values of ionic strength atpase activity stopped rising before hmm was completely saturated with atp. higher ionic strength prevented atpase activity from further increasin ...	2002	126582
renewed voluntary spirit needed, says blue cross;.		2002	139559
[an invariant of plasma viscosity].	determination of "m" invariant cannot lead to an absolute measurement of plasmatic viscosity, because "m" increases with temperature. it allows however to compare data determined in various conditions.	2002	126722
[salt fluoridation in 4 colombian communities. vii. relationship between various nutrients and dental caries].		2002	127591
editorial: medicine-1975.		2002	123904
[incidence and nature of postextraction bacteremia].		2002	127333
[treatment of hemorrhages from esophageal varices].		2003	309021
[study of left ventricular function with quantitative angiocardiographic methods].		2003	161533
the independent living centre.		2003	161503
androblastoma and germ cell tumor of the same testis: a case report.	the rare case of a testicular androblastoma associated with a complex germ cell tumor of the same testis is reported. gynecomastia preceded the clinical appearace of testicular swelling by 3 months, suggesting that the 2 tumors developed contemporaneously and that, in theory, the sertoli cell tumor is probably dependent on the germ cell tumor.	2003	165324
mouth-controlled devices to assist the handicapped.		2003	161985
dental services in commerce.		2003	298676
the structural basis of crystal-induced membranolysis.		2003	181020
[effectiveness of the comprehensive treatment by rhonidase electrophoresis and tbilisi sulfide baths of elderly patients suffering from osteoarthrosis deformans].		2003	154769
serum vitamin a and carotene levels in down's syndrome and other retarded subjects showing enamel abnormalities of the permanent dentition.		2003	151744
[gastroschisis and omphalocele. treatment and results].		2003	150087
pelvic displacement of a cardiac pacemaker following trauma. a case report.	the rate migration of an implanted cardiac pacemaker from the subrectus area anterior to the peritoneum to the pelvis following a motor vehicle accident is reported. discovery of dislodgement was made by observing a marked increase of pacemaker impulse amplitude in the three standard limb leads as measured on an oscilloscope.	2003	152472
[state of the pituitary-adrenal system in burns].		2003	201039
[fate of a lavsan strip used for stenosing the small intestine].		2003	142861
an enzyme histochemical study of non-hodgkin's lymphoma and allied disease.	the enzyme activity for alpha-naphthyl-acetate esterase, naphthol-as-acetate esterase, naphthol-as-d-chloroacetate esterase, acid phosphatase, l(+)-tartrate-resistant acid phosphatase, adenosine triphosphatase, and 5'-nucleotidase was examined on the neoplastic cells of giant follicular lymphoblastoma, the so-called reticulum cell sarcoma and sézary syndrome. the neoplastic cells of giant follicular lymphoblastoma showed distinct activity for adenosine triphosphatase and 5'-nucleotidase, and tho ...	2003	145325
[occupational exogenous siderosis of the skin (author's transl)].		2003	151604