Publications

upper electron-excited states in bioluminescence: experimental indication.	the involvement of upper electron-excited states in bacterial bioluminescence process was studied with excitation energy-accepting molecules. the fluorescent aromatic compounds, anthracene and 1.4-bis(5-phenyloxazol-2-yl)benzene, were chosen. energies of their lowest excited singlet states are higher than the energy of the analogous state of the bioluminescence emitter; their absorption spectra and bioluminescence do not overlap. hence, the excitation of these molecules by singlet-singlet energy ...	2001	11400262
dynamic features of the subunit interface of cu,zn superoxide dismutase as probed by tryptophan phosphorescence.	as part of the more general inquiry on the molecular basis of specific recognition between macromolecules, the subunit-subunit interface structure of dimeric superoxide dismutase from photobacterium leiognathi has been probed selectively by the phosphorescence emission of trp-73, located at the subunit contact region. copper at the catalytic site was found to quench completely the delayed emission and therefore all studies were conducted with the copper-free or cd(2+)-substituted protein. the sp ...	2001	11414691
kinetics of juvenile sea bass (dicentrarchus labrax, l.) systemic and mucosal antibody secreting cell response to different antigens (photobacterium damselae spp. piscicida, vibrio anguillarum and dnp).	the elispot assay was used to measure the number of specific antibody secreting cells (asc) induced during the primary and secondary immune responses in the spleen, head kidney and gut of juvenile (5 g) sea bass (dicentrarchus labrax) to bacterial (vibrio anguillarum and photobacterium damselae ssp. piscicida) and hapten dinitrophenyl-conjugated to keyhole limpet haemocyanin (dnp-klh) antigens administered intraperitoneally. high variability among individuals was observed at each sampling day. a ...	2001	11417719
characterisation of volatile compounds produced by bacteria isolated from the spoilage flora of cold-smoked salmon.	this study investigated the volatile compounds produced by bacteria belonging to nine different bacterial groups: lactobacillus sake, l. farciminis, l. alimentarius, carnobacterium piscicola, aeromonas sp., shewanella putrefaciens, brochothrix thermosphacta, photobacterium phosphoreum and enterobacteriaceae isolated from cold-smoked salmon. each bacterial group was represented by several strains. in addition, combinations of the groups were examined as well. sterile blocks of cold-smoked salmon ...	2001	11428576
[modification of the photobacterium phosphoreum toxicity test method].	in order to reduce the variation of photobacterium phosphoreum luminous intensity during testing, the photobacterium phosphoreum toxicity test was modified by a correction factor, piacetone, in this paper. the effects of magnetic stirring time, culture time, culture generation and culture temperature on the measured results were discussed. the modified method had good laboratory repeatability and the relative standard deviation (rsd) was between 2.1% and 13.1%. reliable data could be obtained us ...	2001	11432056
the acute toxicity of alpha-branched phenylsulfonyl acetates in photobacterium phosphoreum test.	the acute toxicity (15-min ec(50)) of 20 alpha-substituted phenylsulfonyl acetates was measured using the microtox test with photobacterium phosphoreum. on the basis of tlser, quantitative structure-activity relationships (qsars) were calculated for the inhibition of bioluminescence. with the parameters molecular volume (v(mc)), dipolarity/polarizability (pi*), and the covalent contribution to lewis basicity (epsilon(b)), a squared correlation coefficient (r(2)(adj)) of 0.868 and standard error ...	2001	11440477
biodegradation of aliphatic-aromatic copolyesters: evaluation of the final biodegradability and ecotoxicological impact of degradation intermediates.	the biological degradation behaviour of the aliphatic-aromatic copolyester ecoflex was investigated with regard to the degree of degradation and the intermediates formed during the degradation process. the individual thermophilic strain thermomonospora fusca, isolated from compost material, was used for the degradation experiments in a defined synthetic medium at 55 degrees c. after 22 days of degradation more than 99.9% of the polymer had depolymerized and with regard to the degradation of the ...	2001	11444312
characterization of alanine and malate dehydrogenases from a marine psychrophile strain pa-43.	alanine dehydrogenase (aladh: ec 1.4.1.1), malate dehydrogenase (mdh: ec 1.1.1.37), and glutamate dehydrogenase (ec 1.4.1.2), all nad+ dependent, were detected in extracts from a psychrophilic bacterium, strain pa-43, isolated from a sea urchin off the icelandic coast. characterization tests suggested that the strain had a close relationship to vibrio, but sequencing of part of the 16s rdna gene placed the bacterium among shewanella species in a constructed phylogenetic tree. the bacterium had a ...	2001	11453464
vibrio lentus sp. nov., isolated from mediterranean oysters.	twelve phenotypically similar marine bacteria have been studied by means of ribotyping, dna-dna hybridization and cultural and physiological characterization. phylogenetic analysis has been performed of the 16s and 23s rrna genes of two representative strains. phylogenetically, they belong to the vibrio/photobacterium branch of the gamma-proteobacteria and they share all of the properties that define the genus vibrio. the strains represent a new vibrio species that is phenotypically similar to v ...	2001	11491345
kinetic analysis of bacterial bioluminescence in water-organic media.	the interaction of luciferases from two types of luminous bacteria, photobacterium leiognathi and vibrio harveyi, with their substrates [the photorecovered fmnh2 and long-chain aldehydes--decanal (c10), dodecanal (c12) and tetradecanal (c14)] in water-organic media was analysed using kinetic graphical methods. moderate concentrations of organic solvents have been demonstrated to activate the bioluminescence, while higher concentrations inhibit it. the interactions of these effectors with lucifer ...	2001	11512142
fate and effect of monoalkyl quaternary ammonium surfactants in the aquatic environment.	the effect of the alkyl chain of quaternary ammonium-based surfactants on their aquatic toxicity and aerobic biodegradability has been studied. two families of monoalkylquats surfactants were selected: alkyl trimethyl ammonium and alkyl benzyl dimethyl ammonium halides. acute toxicity tests on daphnia magna and photobacterium phosphoreum were carried out and ec50 values in the range of 0.1-1 mg/l were obtained for the two series of cationic surfactants. although the substitution of a benzyl grou ...	2001	11202711
vibrio fischeri outer membrane protein ompu plays a role in normal symbiotic colonization.	the nascent light-emitting organ of newly hatched juveniles of the hawaiian sepiolid squid euprymna scolopes is specifically colonized by cells of vibrio fischeri that are obtained from the ambient seawater. the mechanisms that promote this specific, cooperative colonization are likely to require a number of bacterial and host-derived factors and activities, only some of which have been described to date. a characteristic of many host-pathogen associations is the presence of bacterial mechanisms ...	2001	11673429
expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems.	modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. introduction of the e. coli z905/pphl7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. a low concentration of nutrients has been shown to fav ...	2001	11695439
kinetics of light emission and oxygen consumption by bioluminescent bacteria.	oxygen plays a key role in bacterial bioluminescence. the simultaneous and continuous kinetics of oxygen consumption and light emission during a complete exhaustion of the exogenous oxygen present in a closed system has been investigated. the kinetics are performed with vibrio fischeri, v. harveyi, and photobacterium phosphoreum incubated on respiratory substrates chosen for their different reducing power. the general patterns of the luminescence time courses are different among species but not ...	2001	11710810
development of quantitative structure activity relationships in toxicity prediction of complex mixtures.	to predict the toxicity of mixtures of halogenated benzenes (narcotics).	2001	11730561
characterization of the binding of photobacterium phosphoreum p-flavin by vibrio harveyi luciferase.	the isolated photobacterium phosphoreum luciferase is associated with a bound flavin designated p-flavin and tentatively identified as 6-(3"-myristic acid)-fmn. since fmn and myristic acid are products of the normal luciferase reaction, we explored the possibility that p-flavin can also be bound by luciferase from other luminous bacteria and serve as an active site probe. p-flavin has never been detected in vibrio harveyi cells. we found that the v. harveyi luciferase binds p. phosphoreum p-flav ...	2001	11747297
[photobacterium phosphoreum assay on the toxicity of soil contaminated by heavy metals].	the toxicity of soil artificially contaminated by heavy metals was assessed with photobacterium phosphoreum. the results showed that the duration of optimum balance was 24 hours, and that of optimum extraction was 2 hours. two leaching methods were compared, and data showed that optimum leaching solvent was hydrocarbon chloride with a concentration of 0.1 mol.l-1 hcl. dose-response curve indicated that the content of heavy metals was positively related with the toxicity of soil. the general dete ...	2001	11758433
rna arbitrarily primed pcr survey of genes regulated by toxr in the deep-sea bacterium photobacterium profundum strain ss9.	we are currently investigating the role of toxr-mediated gene regulation in photobacterium profundum strain ss9. ss9 is a moderately piezophilic ("pressure loving") psychrotolerant marine bacterium belonging to the family vibrionaceae. in vibrio cholerae, toxr is a transmembrane dna binding protein involved in mediating virulence gene expression in response to various environmental signals. a homolog to v. cholerae toxr that is necessary for pressure-responsive gene expression of two outer membr ...	2001	11160100
assessing chemical toxicity with the bioluminescent photobacterium (vibrio fischeri): a comparison of three commercial systems.	the inhibition of light emitted by the bioluminescent bacterium, vibrio fischeri, is the basis for several toxicity bioassays. the inhibitory effects of 81 chemicals, after 5 min contact time, were studied at eight concentrations using reagents from three commercial assay systems (toxalert 10, microtox and lumistox). solubility in water was the limiting factor in determining the selection of chemicals for study. the effective nominal concentrations (ec) resulting in 20, 50 and 80% inhibition wer ...	2001	11547867
[effect of anaerobic acidification treatment on nitrogen removal and toxicity reduction of coke plant wastewater].	coke plant wastewater was treated by anaerobic acidification-anoxic-aerobic (a1-a2-o) biofilm process and anoxic-aerobic (a/o) biofilm process respectively. toxicity reduction effects of the two processes were compared. the toxicity of coke plant wastewater was equal to that of mercury chloride with concentration of 0.19 mg/l. its toxicity reduction was related to the removal of organic nitrogen. anaerobic acidification unit acted an important role on removal of both organic nitrogen and reducti ...	2001	11569122
toxicity and hazardous properties of solvent base adhesive wastes.	in this work, the hazardous properties of solvent base adhesive wastes generated in the footwear manufacturing process have been studied. the characterisation procedures and criteria used are those contained in the legal documents european union council decision 94/904/ce and october 13th spanish ministerial order. the properties studied were the following: flash point, reactivity (gas generation), ecotoxicity, main contaminants extracted by the leaching process and main harmful substances conta ...	2001	11954730
characterization of psychrotrophic bacteria in the surface and deep-sea waters from the northwestern pacific ocean based on 16s ribosomal dna analysis.	seventy-eight 4 degrees c-culturable bacteria were isolated using zobell 2216e medium from surface (0-200 m) and deep-sea (1000-9671 m) waters in the northwestern pacific ocean. growth studies indicated that all 4 degrees c-culturable bacteria were psychrotrophs. six phylotypes were observed in the surface water samples and 8 phylotypes in the deep-sea waters. phylogenetic characterization based on 16s ribosomal dna sequence analysis of the representative phylotypes revealed that some bacterial ...	2001	14961338
statistical optimization of bioluminescence of photobacterium phosphoreum kctc2852.	in this study, media optimization by statistically designed experiments stimulated an increase in the intensity and stability of bioluminescence of photobacterium phosphoreum kctc 2852 during continuous cultivation. the plackett-burman design method selected two components (nacl, yeast extract) among nine components of the seawater complete (sc) medium. the response-surface design method calculated the optimum concentration of nacl (31.1 g/l) and yeast extract (5.1 g/l). in the optimized medium, ...	2001	16233061
[study of the binding between bacterial lipopolysaccharides and polymyxin by a bioluminescent method].	for rating the interaction of lipopolysaccharides (lps) with polymixin b (pmb) a bacterial bioluminescence is offered to be used. bioluminescence level of bacteria decreases under free antibiotic amounts action. it is shown, that as a result of interaction with lps antibiotic properties of pmb are reduced, and the intensity of bacterial bioluminescence is restored. the bioluminescence level in such system characterizes the lps quantity. kinetic properties of bacterial light emission at the prese ...	2002	12924022
effects of potassium halides on bacterial bioluminescence.	the effects of potassium halides kcl, kbr and ki on nadh:fmn-oxidoreductase-luciferase bioluminescent coupled enzyme system were studied. the influence of salt additions on bioluminescence intensity and bioluminescence light yield was investigated. the inhibition and activation parameters of the salts were calculated using their dependencies on concentration of the salts. the correlation between the inhibition of bioluminescence intensity and the halide mass was demonstrated: the inhibiting abil ...	2002	11960733
[characteristics and utilities of luminescent bacteria from the black sea].	luminescent bacteria, isolated from summer specimens of water of the black sea, have been identified as strains of photobacterium phosphoreum and vibrio fischeri (two of each). morphological, physiological, and biochemical properties of the four strains have been characterized, and the kinetic behavior of luciferases isolated therefrom has been studied. the sensitivity of the luminescence of the strains to certain toxic agents has been compared to that of the test strain ph. phosphoreum (cohn) f ...	2002	11962223
microbial spoilage and formation of biogenic amines in fresh and thawed modified atmosphere-packed salmon (salmo salar) at 2 degrees c.	to evaluate the microbial spoilage, formation of biogenic amines and shelf life of chilled fresh and frozen/thawed salmon packed in a modified atmosphere and stored at 2 degrees c.	2002	11966922
sensory, chemical and bacteriological changes during storage of iced squid (todaropsis eblanae).	to relate sensory shelf-life of iced whole and gutted squid to bacterial growth and chemical changes.	2002	11972700
binding of haemin by the fish pathogen photobacterium damselae subsp. piscicida.	whole cells of virulent (di 21 and b 51) and avirulent (atcc 29690 and epoy 8803-ii) strains of photobacterium damselae subsp. piscicida, grown under iron-supplemented or iron-restricted conditions, were able to bind haemin. iron limitation resulted in an increased binding of haemin by di 21, b 51 and atcc 29690 cells but did not affect the haemin-binding ability of the epoy 8803-ii cells. proteinase k treatment of whole cells markedly reduced the binding of haemin, indicating that protein recep ...	2002	12005232
host-symbiont recognition in the environmentally transmitted sepiolid squid-vibrio mutualism.	associations between environmentally transmitted symbionts and their hosts provide a unique opportunity to study the evolution of specificity and subsequent radiation of tightly coupled host-symbiont assemblages [3, 8, 24]. the evidence provided here from the environmentally transmitted bacterial symbiont vibrio fischeri and its sepiolid squid host (sepiolidae: euprymna) demonstrates how host-symbiont specificity can still evolve without vertical transmission of the symbiont [1]. infection by in ...	2002	12019463
photobacterium damselae ssp. piscicida: detection by direct amplification of 16s rrna gene sequences and genotypic variation as determined by amplified fragment length polymorphism (aflp).	a pcr protocol for the rapid diagnosis of fish 'pasteurellosis' based on 16s rrna gene sequences was developed. the procedure combines low annealing temperature that detects low titers of photobacterium damselae but also related species, and high annealing temperature for the specific identification of p. damselae directly from infected fish. the pcr protocol was validated on 19 piscine isolates of p. damselae ssp. piscicida from different geographic regions (japan, italy, spain, greece and isra ...	2002	12033705
structure and regulation of the omega-3 polyunsaturated fatty acid synthase genes from the deep-sea bacterium photobacterium profundum strain ss9.	omega-3 polyunsaturated fatty acids (pufas) such as eicosapentaenoic acid (20:5n-3; epa) and docosahexaenoic acid (22:6n-3; dha) have been shown to be of major importance in the promotion of cardiovascular health, proper human development and the prevention of some cancers. a high proportion of bacterial isolates from low-temperature and high-pressure marine environments produce epa or dha. this paper presents the sequence of a 33 kbp locus from the deep-sea bacterium photobacterium profundum st ...	2002	12055309
a case study of logistic qsar modeling methods and robustness tests.	acute toxicity (15-min ec(50)) determination of 16 substituted naphthalene compounds to photobacterium phosphoreum was undertaken according to the standard procedures, while the effects of molecular structures of selected compounds on their toxicity to test microorganisms were logistically conducted using the quantitative structure-activity relationship (qsar) technique. the relationship was developed as -log ec(50)=5.5916 (+/-0.1189)-7.4893(+/-0.4900)qh(+)+0.7771(+/-0.0619e(lumo)+0.0088(+/-0.00 ...	2002	12061832
biological properties of arginine-based gemini cationic surfactants.	biological properties of novel gemini (double-chain/double-head) cationic surfactants, nalpha,nomega-bis(nalpha-acylarginine)alpha,omega-alkylendiamides, so-called bis(args), are reported. the effect of both the alkyl (10 and 12 carbon atoms) and the spacer chain (from 2-10 methylene groups) of bis(args) on their antimicrobial activity, acute toxicity on daphnia magna and photobacterium phosphoreum, and aerobic biodegradability is studied. these surfactants constitute a novel class of chemicals ...	2002	12069315
photobacterium damselae subsp. piscicida: an integrated view of a bacterial fish pathogen.	pasteurellosis, or pseudotuberculosis, is a bacterial septicaemia caused by the halophilic bacterium photobacterium damselae subsp. piscicida (formerly pasteurella piscicida). although this disease was first described in wild populations of white perch and striped bass, currently the natural hosts of the pathogen are a wide variety of marine fish. the disease has great economic impact both in japan, where it affects mainly yellowtail cultures, and in the mediterranean area, due to the losses it ...	2002	12102234
a novel and sensitive test for rapid determination of water toxicity.	the performance of a novel, rapid, and sensitive test for detecting chemical toxicants in water is described in this article. the bioassay utilizes a highly sensitive variant of the luminescent bacterium photobacterium leiognathi that allows the detection in water at levels below milligrams per liter of diverse groups of toxicants, including heavy metals, pesticides, pcbs, polycyclic aromatic hydrocarbons, and fuel traces. for most toxic agents reported in this study, the new assay was markedly ...	2002	12112638
implications of the reactive thiol and the proximal non-proline cis-peptide bond in the structure and function of vibrio harveyi luciferase.	the role of a highly reactive cysteine residue, cys106, in vibrio harveyi luciferase in modulating the substrate-enzyme interactions and in turn affecting the enzyme activity has been extensively investigated over the past three decades. replacing cys106 with valine dramatically hinders the ability of luciferase to stabilize the c4a-hydroperoxyflavin intermediate [abu-soud, h. m., clark, a. c., francisco, w. a., baldwin, t. o., and raushel, f. m. (1993) j. biol. chem. 268, 7699-7706] and consume ...	2002	12146958
bacterial flora from the gut of the wild and cultured banana prawn, penaeus merguiensis.	there is growing awareness of the influence of the bacterial composition of the gut on the health and growth of the host. this study compared the bacterial flora from the digestive system of the wild and cultured prawn, penaeus merguiensis.	2002	12147069
cloning and expression of the haloalkane dehalogenase gene dhma from mycobacterium avium n85 and preliminary characterization of dhma.	haloalkane dehalogenases are microbial enzymes that catalyze cleavage of the carbon-halogen bond by a hydrolytic mechanism. until recently, these enzymes have been isolated only from bacteria living in contaminated environments. in this report we describe cloning of the dehalogenase gene dhma from mycobacterium avium subsp. avium n85 isolated from swine mesenteric lymph nodes. the dhma gene has a g+c content of 68.21% and codes for a polypeptide that is 301 amino acids long and has a calculated ...	2002	12147465
bioremediation of soil contaminated with alkyllead compounds.	sandy soil, which was highly contaminated with alkyllead compounds, was taken from bore cores from a site of a former tetraalkyllead producing company. it was analyzed for its capacity to chemically and/or biologically degrade alkyllead contaminants. for this purpose, soil samples were supplied with oxygen or oxygen + minerals at different water saturation. for long-term elution, contaminated soil was packed into glass columns of 1.5m length and 10cm diameter. oxygen-saturated water was recircul ...	2002	12171412
induction of light emission by luminescent bacteria treated with uv light and chemical mutagens.	intensity of light emission by luminescent bacteria in response to uv irradiation and chemical mutagens was tested. we demonstrated that luminescence of six strains of marine bacteria (belonging to four species: photobacterium leiognathi, p. phosphoreum, vibrio fischeri and v. harveyi) is significantly increased by uv irradiation relatively shortly after dilution of cultures. such a stimulation of luminescence was abolished in cells treated with chloramphenicol 15 min before uv irradiation, indi ...	2002	12177528
ecotoxicological assessment of leachates from mswi bottom ashes.	in this paper, chemical and ecotoxicological data of leachates from bottom ashes collected in different municipal solid waste incinerators (mswi) are shown. the bottom ashes were collected in belgium (three incinerators--samples b1 to b3), france, germany, italy and united kingdom (one incinerator in each country--samples f1, d1, i1 and uk1, respectively). both chemical and ecotoxicological characterizations of leachates were done on the framework of the european directive 91/689/eec and the eur ...	2002	12214969
[toxicity effect of substituted benzenes in oilfield wastewater by molecular orbital method].	the quantum parameters of energy of highest occupied molecular orbital (ehomo), energy of lowest unoccupied molecular orbital (elumo), energy of next highest occupied molecular orbital (enhomo), energy of next lowest unoccupied molecular orbital (enlumo), heat of molecular formation(delta h0f) and dipole moment (mu) of 55 substituted benzenes are calculated based on mopac software of molecular orbital am1 method. the qsar model of multiple descriptors was established by the above parameters and ...	2002	12222057
amplified fragment length polymorphism (aflp) and biochemical typing of photobacterium damselae subsp. damselae.	the aim of the present study was to characterize subspecifically photobacterium damselae subsp. damselae strains isolated from cultured sparus aurata and dicentrarchus labrax by means of phenotypic and molecular typing techniques (amplified fragment length polymorphism, aflp).	2002	12234352
the professional phagocytes of sea bass (dicentrarchus labrax l.): cytochemical characterisation of neutrophils and macrophages in the normal and inflamed peritoneal cavity.	in order to identify the phagocytic cells of sea bass, the peritoneal leucocyte population of fish injected intraperitoneally with photobacterium damselae subspecies piscicida was studied by light microscopy using cytocentrifuge preparations stained by the antonow technique for peroxidase detection. among the leucocytes present in the peritoneal exudate of the infected fish (macrophages, neutrophils, eosinophilic granular cells, lymphocytes and thrombocytes), macrophages and neutrophils were the ...	2002	12365730
role of hsp70 (dnak-dnaj-grpe) and hsp100 (clpa and clpb) chaperones in refolding and increased thermal stability of bacterial luciferases in escherichia coli cells.	the role of chaperones hsp70 (dnak-dnaj-grpe) and hsp100 (clpa-clpb-clpx) in refolding of thermoinactivated luciferase from the marine bacterium photobacterium fischeri and the terrestrial bacterium photorhabdus luminescens has been studied. these luciferases are homologous, but differ greatly in the rate of thermal inactivation and the rate constant for the luminescence reaction. it was shown that refolding of thermoinactivated luciferases is completely determined by the dnak-dnaj-grpe system. ...	2002	12387711
diffusion of beta-lactam antibiotics through oligomeric or monomeric porin channels of some gram-negative bacteria.	the penetration of anionic beta-lactam antibiotics through porins was evaluated as a mechanism of drug resistance. the major proteins with porin activity were purified from the outer membranes of six bacteria. three of the six porins were oligomeric porins. the molecular weights of their monomers were 37 kda from photobacterium damsela, 42 kda from serratia liquefaciens, and 36 kda from e. coli b. the other three porins were heat-modifiable monomeric porins with molecular weights of 43 kda from ...	2002	12402087
hydrogen production by cyanobacteria in an automated outdoor photobioreactor under aerobic conditions.	the possibility of hydrogen production by a hydrogenase impaired mutant strain of anabaena variabilis in outdoor culture was studied. a computer-controlled rooftop (outdoor) tubular photobioreactor (4.35 l) was assembled. h(2) production rates by a. variabilis pk84 grown in co(2) + air in the photobioreactor were measured together with other parameters such as temperature, irradiance, ph, dry biomass weight, and po(2), and chl a concentrations during summer months of 1998 and 1999. efficiencies ...	2002	12402323
seasonality of chesapeake bay bacterioplankton species.	bacteria, gamma-subclass of proteobacteria, vibrio-photobacterium, vibrio vulnificus, vibrio cholerae-vibrio mimicus, and vibrio cincinnatiensis in water samples collected from the choptank river in chesapeake bay from 15 april to 16 december 1996 were enumerated using a fluorescent oligonucleotide direct-counting (fodc) procedure. fodc results obtained using a bacteria taxon-specific probe ranged from one-third the number of to the same number as that obtained by the acridine orange direct coun ...	2002	12406742
bacteria of the gamma-subclass proteobacteria associated with zooplankton in chesapeake bay.	the seasonal abundance of gamma-subclass proteobacteria, vibrio-photobacterium, vibrio cholerae-vibrio mimicus, vibrio cincinnatiensis, and vibrio vulnificus in the choptank river of chesapeake bay associated with zooplankton was monitored from april to december 1996. large (>202- microm) and small (64- to 202- microm) size classes of zooplankton were collected, and the bacteria associated with each of the zooplankton size classes were enumerated by fluorescent oligonucleotide direct count. larg ...	2002	12406743
[interaction of cationic surface-active antiseptics and serum albumin by the bacterial bioluminescence method].	human serum albumin binding of cationic surface-active antiseptics was studied using a photobacterium phosphoreum k3 strain isolated by the authors in the black sea. binding of cationic surface-active proteins to serum albumin led to disappearance of toxic and antibacterial characteristics of miramistine, ethonium, and decametoxin, but not chlorohexidine. the activity of chlorohexidine was reduced by 45-48% in the presence of albumin. albumin binding capacity towards miramistine was 7.0-8.7%, to ...	2002	12412506
stimulated biosynthesis of flavins in photobacterium phosphoreum ifo 13896 and the presence of complete rib operons in two species of luminous bacteria.	photobacterium phosphoreum ifo 13896 emits light strongly when cultured in medium containing 3% nacl, but only weakly in medium containing 1% nacl. it is known that dim or dark mutants appear frequently and spontaneously from this parent strain. to confirm that riboflavin biosynthesis is stimulated when the lux operon is active, the amount of light emitted and flavins synthesized under strongly or weakly light emitting conditions was determined. in comparison with the parent strain cultured in 3 ...	2002	12444973
qsar of ecotoxicological data on the basis of data-driven if-then-rules.	a rather small data matrix of seven chemicals and 17 different ecotoxicological end points is examined by methods of discrete mathematics. especially, the lattice theory and its variant, the formal concept analysis may be an attractive tool to analyze quantitative structure activity relationships, when a numerical functional approach is not at hand. the central item is the so called concept, which is a pair of subsets: a subset of molecules and a subset of properties which correspond to each oth ...	2002	12463679
solute accumulation in the deep-sea bacterium photobacterium profundum.	the identity and amounts of intracellular solutes in the deep-sea bacterium photobacterium profundum strain ss9 were studied using nuclear magnetic resonance techniques. p. profundum strain ss9, a moderate piezophile which grows optimally at 20-30 mpa primarily accumulated glutamate and betaine, with lesser amounts of alanine, beta-hydroxybutyrate (beta-hb) and oligomers composed of the beta-hb units when grown at 0.1 mpa to early stationary phase. when grown at the optimal pressure, the cells p ...	2002	12486460
comparative study of four qsar models of aromatic compounds to aquatic organisms.	quantitative structure-activity relationships (qsars) were developed for 43 aromatic compounds toxicity to photobacterium phosphoreum and daphnia magna based on four methods: octanol/water partition coefficient, linear solvation energy relationship, molecular connectivity index and group contribution. through the evaluation of four qsar methods, lser was proved to be the best. and it applied to the widest range of chemicals with the greatest accuracy.	2002	12491732
bioluminescence assays: effects of quinones and phenols.	the influence of a series of quinones and phenols on bacteria bioluminescence systems was investigated. three bioluminescence systems used in ecological monitoring were compared: (1) water-soluble; (2) immobilized in starch gel coupled enzyme systems: nadh:fmn-oxidoreductase-luciferase; (3) luminescent bacteria. bioluminescence inhibition constants of quinones and phenols and bioluminescence induction periods were compared. these kinetic parameters are proportional to quinone concentrations and ...	2002	12568457
reversion mutation in dark variants of luminous bacteria and its application in gene toxicant monitoring.	the luminous intensity of dark variant (s1) separated from photobacterium phosphoreum (a2) was 1/10,000 less than that of wild-type. ethidium bromide (eb) (0.6 mg/l), mytomycin c (mc, 0.05 mg/l), 2-amino fluorene (2-af, 1.0 mg/l) all could strongly induce reversion mutation for s1 within 24 h and increase reversion ratio significantly. the results of experiments indicated that these revertants had stable genetic characteristic and the mutation may take place at gene levels. the mutagenesis to s1 ...	2002	12658797
the cytotoxic and genotoxic potential of surface water and wastewater effluents as determined by bioluminescence, umu-assays and selected biomarkers.	two bacterial tests employing photobacterieum phosphoreum (microtox bioluminescence test) and salmonella typhimurium ta 1535 psk1002 (umu-assay) were evaluated to estimate the cytotoxic and genotoxic potential of water samples from the selected rivers in germany as well as the primary and secondary effluents of some sewage treatment plants. rainbow trout (onchorynchus mykiss) were exposed to different concentrations (20-40%) of secondary effluent in the model online aquatic monitoring plant wabo ...	2002	11827279
variation of toxicity during the ozonation of monochlorophenolic solutions.	this study investigates the variation of toxicity during ozonation of 2-chlorophenol (2-cp), 3-chlorophenol (3-cp) and 4-chlorophenol (4-cp) in neutral condition. acute toxicity of pure chlorophenols (cps) and their ozonated intermediates was evaluated by microtox assay. the results revealed that the intermediates of oxidized cps induced new toxicity during the early stage of ozonation, and the ozonated 2-cp showed higher degree of toxicity increase than 3-cp and 4-cp. the maximum toxicity norma ...	2002	11846283
antimicrobial effect of essential oils on the seafood spoilage micro-organism photobacterium phosphoreum in liquid media and fish products.	to evaluate the antimicrobial effect of nine essential oils (eo) on p. phosphoreum and determine the effect of oregano oil on the shelf-life of modified atmosphere-packed (map) cod fillets.	2002	11849488
nad(p)h:flavin mononucleotide oxidoreductase inactivation during 2,4,6-trinitrotoluene reduction.	bacteria readily transform 2,4,6-trinitrotoluene (tnt), a contaminant frequently found at military bases and munitions production facilities, by reduction of the nitro group substituents. in this work, the kinetics of nitroreduction were investigated by using a model nitroreductase, nad(p)h:flavin mononucleotide (fmn) oxidoreductase. under mediation by nad(p)h:fmn oxidoreductase, tnt rapidly reacted with nadh to form 2-hydroxylamino-4,6-dinitrotoluene and 4-hydroxylamino-2,6-dinitrotoluene, wher ...	2002	11916686
emission of polycyclic aromatic hydrocarbons, toxicity, and mutagenicity from domestic cooking using sawdust briquettes, wood, and kerosene.	smoke samples, in both gas and particulate matter (pm) phases, of the three domestic stoves were collected using u.s. epa modified method 5 and were analyzed for 17 pah (hplc-uv), acute toxicity (microtox test), and mutagenicity (amestest). the gas phase of smoke contributed > or = 95% of 17 pah, > or = 96% of toxicity, and > or = 60% of mutagenicity. the highest emission factor of 17 pah was from sawdust briquettes (260 mg/kg), but the highest emission of 11 genotoxic pah was from kerosene (28 ...	2002	11918004
toxicity and color formation during ozonation of mono-substituted aromatic compounds.	this research investigates the aqueous toxicity occurring during color formation due to ozonation in a semi-batch reactor. the tested mono-substituted aromatic compounds were aniline, phenol (-nh2' -oh, electron-donating group), nitrobenzene and benzoic acid (-no2' -cooh, electron-withdrawing group). the results revealed that the aqueous toxicity of the microtox assay from oxidized samples during the early stage of ozonation increased when the color formation was observed, except in the case of ...	2002	11918401
sensory, chemical and bacteriological changes in vacuum-packed pressurised squid mantle (todaropsis eblanae) stored at 4 degrees c.	sensory, chemical and bacteriological changes were studied in vacuum-packed squid mantles (todaropsis eblanae) that were pressurised at 150, 200, 300 and 400 megapascal (mpa) for 15 min at ambient temperature and stored at 4 degrees c. sensory analysis showed that the higher the pressurisation the longer the shelf-life. thus, the lot pressurised at 400 mpa was rejected after 28 days' storage compared with 7 days for the untreated lot. the chemical results generally corresponded with the sensory ...	2002	11929163
an ecotoxic risk assessment of residue materials produced by the plasma pyrolysis/vitrification (pp/v) process.	plasma is the fourth state of matter, following the three states of solid, liquid and gas. experience has amply demonstrated that solids exposed to the oxygen-deficient plasma flame are converted to liquid, and liquid exposed to the same flame is converted to gas. a low amount of vitrified solid residue material usually remains at the end of this process. plasma pyrolysis/vitrification (pp/v) has been demonstrated as a safe, efficient, cost-effective technology for the treatment of wastes, inclu ...	2002	11952180
use of partition coefficients to predict mixture toxicity.	by using the c(18)-empore disks/water partition coefficient (k(md)) to describe the toxicity of 50 mixed halogenated benzenes to photobacterium phosphoreum, an approach is proposed in this study. application of the approach to the 15 other related mixtures prove the predictive capability of this k(md)-based approach, due to the consistency between the predicted toxicity and the observed ones with r(2)=0.929, se=0.104, f=169.513 at p<0.001. further analysis of this approach finds that, for the mi ...	2003	12691908
interaction of aromatic compounds with photobacterium leiognathi luciferase: fluorescence anisotropy study.	the time-resolved and steady-state fluorescence techniques were employed to elucidate possible interactions of four aromatic compounds (anthracene, popop, msb and 1,4-naphthalendiol) with bacterial luciferase. fluorescence spectra and fluorescence anisotropy decays of these compounds were studied in ethanol, water-ethanol solutions and in the presence of bacterial luciferase. shifts of fluorescent spectra and differences in rotational correlation times are interpreted in terms of weak (hydrophob ...	2003	12701091
the acute toxicity of gluconic acid, beta-alaninediacetic acid, diethylenetriaminepentakismethylenephosphonic acid, and nitrilotriacetic acid determined by daphnia magna, raphidocelis subcapitata, and photobacterium phosphoreum.	acute toxicity of four relatively new chelating agents and their equimolar manganese and cadmium complexes was studied. the chelating agents studied were gluconic acid (ga), beta-alaninediacetic acid (ada), diethylenetriaminepentakismethylenephosphonic acid (dtpmp), and nitrilotriacetic acid (nta). three common bioassays, namely daphnia magna, raphidocelis subcapitata, and photobacterium phosphoreum (microtox bioassay) were applied. r. subcapitata proved the most sensitive to these compounds. wi ...	2003	12712292
pharmacokinetics of flumequine and in vitro activity against bacterial pathogens of gilthead sea bream sparus aurata.	the present study investigated the kinetic profile of flumequine (flu) in gilthead sea bream sparus aurata (170 g) held at 19 degrees c and evaluated its in vitro efficacy against important bacterial diseases in mediterranean mariculture. following a single intravascular injection (10 mg kg(-1) fish), the distribution half-life (t1/2alpha) and the half-life of the terminal phase of elimination (t1/2gamma) of the drug were 0.2 and 30 h respectively. tissue penetration of flu was low, since both t ...	2003	12718468
standardized system for quantifying residual dirt on medical appliances cleansed in hospital washers--disinfectors: dirt detection by different methods.	an easy-to construct, easy-to-operate standardized system was developed for determining the residual biological contamination of surgical instruments, endoscopes and other medical appliances subjected to hospital cleansing and/or disinfection. it consists of standard-sized pieces of glass, metal or endoscope plastic--dirt carriers--either bare or enclosed in truncated eppendorf caps to simulate hard-to-access conditions. the surface of the carriers is covered with model dirt simulating biologica ...	2003	12744084
carriage of potentially fish-pathogenic bacteria in sparus aurata cultured in mediterranean fish farms.	a bacteriological survey of gilthead sea bream sparus aurata from different fish farms and culture systems on the spanish mediterranean coast was conducted. three different studies were performed. study a included hatchery-reared larvae; study b, periodic examination of randomly sampled growing fish; and study c, growing fish sampled only during mortality/morbidity events. in studies b and c, sea cages, earth ponds and indoor tanks were surveyed, and in both cases diseased (showing clinical sign ...	2003	12747637
acute and chronic toxicity of dimethylsulfoxide to daphnia magna.		2003	12756470
static and dynamic water molecules in cu,zn superoxide dismutase.	understanding protein hydration is a crucial, and often underestimated issue, in unraveling protein function. molecular dynamics (md) computer simulation can provide a microscopic description of the water behavior. we have applied such a simulative approach to dimeric photobacterium leiognathi cu,zn superoxide dismutase, comparing the water molecule sites determined using 1.0 ns md simulation with those detected by x-ray crystallography. of the water molecules detected by the two techniques, 20% ...	2003	12784219
biodegradation of plasticizers by rhodotorula rubra.	the degradation of plasticizers by the yeast rhodotorula rubra j-96-1 (american type culture collection 9449) in the presence of glucose was studied. the plasticizers included the commonly used bis-2-ethylhexyl adipate (b(eh)a), dioctyl phthalate (dop), and dioctyl terephthalate (dotp), and the less commonly used dipropylene glycol dibenzoate (d(pg)db) and diethylene glycol dibenzoate (d(eg)db). the proposal had been made that the latter two plasticizers be used as alternatives to the first thre ...	2003	12785580
[the inhibitory effect of extracts from galla chinensis on marine fouling bacteria].	to observe the activity of extracts from galla chinensis with different solvents against marine fouling bacteria in vitro.	2003	12795221
quantification of joint effect for hydrogen bond and development of qsars for predicting mixture toxicity.	a qsar model is successfully proposed to predict the toxicity effect on photobacterium phosphoreum by nonpolar-narcotic-chemical mixtures and/or polar-narcotic-chemical mixtures. for nonpolar-narcotic-chemical mixtures and polar-narcotic-chemical mixtures, their corresponding hydrophobicity-based qsar models are derived from regression analysis. comparison of these two qsar models make us believe that it is the joint effect of hydrogen bond in polar-narcotic-chemical mixture that leads to the di ...	2003	12821001
apoptosis of sea bass (dicentrarchus labrax l.) neutrophils and macrophages induced by experimental infection with photobacterium damselae subsp. piscicida.	the infection of sea bass (dicentrarchus labrax l.) by intraperitoneal (i.p.) injection of the agent of fish pasteurellosis photobacterium damselae subsp. piscicida resulted in the apoptosis of peritoneal neutrophils and macrophages. all the eight virulent and none of the two non-virulent strains tested exhibited apoptogenic activity. a secreted bacterial protein(s) is a likely candidate as the factor(s) responsible for this activity, since no apoptosis was induced by i.p. injected uv-killed vir ...	2003	12834617
[detection of heterotrophic bacteria and vibrios in shoal of bullacta exarata].	the number of heterotrophic bacteria and vibrios in shoal for bullacta exarata rearing fluctuated mainly within 1.25 x 10(4)-7.21 x 10(4) cfu.g-1 and 0-1.08 x 10(4) cfu.g-1, respectively. 173 strains of bacteria, mainly belong to 13 genera were isolated. the predominant genera were composed of bacillus, photobacterium, pseudomonas, vibrio and some genera of enterobacteriaceae. the number of bacteria changed in line with the alteration of the temperature, and was especially affected by the organi ...	2003	12836568
dominance of vibrio fischeri in secreted mucus outside the light organ of euprymna scolopes: the first site of symbiont specificity.	previous studies of the euprymna scolopes-vibrio fischeri symbiosis have demonstrated that, during colonization, the hatchling host secretes mucus in which gram-negative environmental bacteria amass in dense aggregations outside the sites of infection. in this study, experiments with green fluorescent protein-labeled symbiotic and nonsymbiotic species of gram-negative bacteria were used to characterize the behavior of cells in the aggregates. when hatchling animals were exposed to 10(3) to 10(6) ...	2003	12839763
toxicity of nitric oxide and peroxynitrite to photobacterium damselae subsp. piscicida.	virulent strains of photobacterium damselae subsp. piscicida (pdp) were grown in media with or without glucose supplementation (to enhance polysaccharide capsule formation) and the bactericidal action of nitric oxide (no) and peroxynitrites was evaluated in a cell-free assay. treatment with the no-donor s-nitroso-acetyl-penicillamine (snap) induced a dose-and time-dependent decrease in pdp survival. this effect was greater for strains grown without glucose supplementation (c forms) than for thei ...	2003	12892745
active-site copper and zinc ions modulate the quaternary structure of prokaryotic cu,zn superoxide dismutase.	the influence of the constitutive metal ions on the equilibrium properties of dimeric photobacterium leiognathi cu,zn superoxide dismutase has been studied for the wild-type and for two mutant protein forms bearing a negative charge in the amino acid clusters at the dimer association interface. depletion of copper and zinc dissociates the two mutant proteins into monomers, which reassemble toward the dimeric state upon addition of stoichiometric amounts of zinc. pressure-dependent dissociation i ...	2003	12595249
patch-clamp experiments with porins extracted from a marine bacterium (photobacterium profundum strain ss9) and reconstituted in liposomes.	the reconstitution of bacterial porins in liposome bilayers for patch-clamp recording is well established. however, the solutions used in the dehydration, rehydration, and osmotic swelling of the liposomes have been developed for porins from enteric bacteria. porins from marine bacteria normally function in contact with seawater whose ionic composition and osmotic pressure would appear to be incompatible with the established methods. here, we show that, contrary to expectation, an established re ...	2003	12625625
multiple computer-automated structure evaluation study of aquatic toxicity. iii. vibrio fischeri.	an acute toxicity model was constructed on the basis of 901 chemicals tested for toxicity against the luminescent bacteria vibrio fischeri (formerly photobacterium phosphoreum, the microtox test). the model was created using the multiple computer-automated structure evaluation (m-case) program. the model can correctly predict acute toxicity for 92% of the compounds with an error averaging 0.55 log units per median effect concentration (ec50). the main toxicophores, corresponding to polar and non ...	2003	12627631
cloning and nucleotide sequence analysis of the chloramphenicol resistance gene on conjugative r plasmids from the fish pathogen photobacterium damselae subsp. piscicida.	transferable resistance to various drugs was investigated in photobacterium damselae subsp. piscicida from japan. drug resistances were transferred via plasmids of 100, 50, and 40 kb. resistance to chloramphenicol (cmr) was transferred on plasmids of all 3 sizes. the cmr gene (cat) was cloned from the 50 kb plasmids ppdp8511 and ppdp9106 transferred from p. damselae subsp. piscicida strains isolated in different years and places in japan. subcloning localized the cat to within 1.5 kb hindiii-hin ...	2003	12650243
concerted simulations reveal how peroxidase compound iii formation results in cellular oscillations.	a major problem in mathematical modeling of the dynamics of complex biological systems is the frequent lack of knowledge of kinetic parameters. here, we apply brownian dynamics simulations, based on protein three-dimensional structures, to estimate a previously undetermined kinetic parameter, which is then used in biochemical network simulations. the peroxidase-oxidase reaction involves many elementary steps and displays oscillatory dynamics important for immune response. brownian dynamics simul ...	2003	12944259
similarity of tetracycline resistance genes isolated from fish farm bacteria to those from clinical isolates.	tetracycline-resistant (tet(r)) bacteria were isolated from fishes collected at three different fish farms in the southern part of japan in august and september 2000. of the 66 tet(r) gram-negative strains, 29 were identified as carrying tetb only. four carried tety, and another four carried tetd. three strains carried tetc, two strains carried tetb and tety, and one strain carried tetc and tetg. sequence analyses indicated the identity in tet(r) genes between the fish farm bacteria and clinical ...	2003	12957921
investigation of media formulations promoting differential antigen expression by photobacterium damsela ssp. piscicida and recognition by sea bass, dicentrarchus labrax (l.), immune sera.	photobacterium damsela ssp. piscicida (phdp) isolates were grown in various bacteriological media, in eukaryotic cell culture media and in the presence of fish cells (resembling some aspects of in vivo growth environments). bacterial cells, extracellular products (ecps) and crude capsular polysaccharide were isolated and analysed by electrophoresis and western blot using sea bass sera. growth in bacteriological media conserved the synthesis of cell and extracellular components when these were co ...	2003	12962207
vaccination trials of sea bass, dicentrarchus labrax (l.), against photobacterium damsela subsp. piscicida, using novel vaccine mixtures.	bacterial cells of the marine fish pathogen photobacterium damsela subsp. piscicida were grown in novel culture media. a mixture of whole cells and extracellular components was inactivated and used in bath, intraperitoneal (i.p.) and oral vaccination of sea bass, dicentrarchus labrax, employing two sizes of fish. a commercial vaccine was used for comparative purposes. control and immunized fish were either bath or intraperitoneally challenged 6 and 12 weeks post-vaccination. small fish had signi ...	2003	12962216
survival against exogenous hydrogen peroxide of photobacterium damselae subsp. piscicida under different culture conditions.		2003	12962239
the sos-lux-lac-fluoro-toxicity-test on the international space station (iss).	in the 21st century, an increasing number of astronauts will visit the international space station (iss) for prolonged times. therefore it is of utmost importance to provide necessary basic knowledge concerning risks to their health and their ability to work on the station and during extravehicular activities (eva) in free space. it is the aim of one experiment of the german project triple-lux (to be flown on the iss) to provide an estimation of health risk resulting from exposure of the astrona ...	2003	12971406
oxidative stress monitoring in biological samples by bioluminescent method.	the integral bioluminescent biotest with lyophilized fluorescent bacteria was used for monitoring of lpo processes in tissue extracts and serum of rats exposed to stress. a relationship between the content of mda (lpo indicator) and fluorescence of bacteria was observed in all biological samples.	2003	14631513
simple and rapid detection of photobacterium damselae ssp. piscicida by a pcr technique and plating method.	to detect photobacterium damselae ssp. piscicida using the pcr technique and plating method.	2003	14633013
accuracy of six commercially available systems for identification of members of the family vibrionaceae.	six commercially available bacterial identification products were tested with vibrio alginolyticus (12 strains), v. cholerae (30 strains), photobacterium (vibrio) damselae (10 strains), v. fluvialis (10 strains), v. furnissii (4 strains), v. hollisae (10 strains), v. metschnikovii (9 strains), v. mimicus (10 strains), v. parahaemolyticus (30 strains), and v. vulnificus (10 strains) to determine the accuracy of each system for identification. the products included api 20e, crystal e/nf, microscan ...	2003	14662957
three-dimensional quantitative structure-activity relationship study for phenylsulfonyl carboxylates using comfa and comsia.	from both the comparative molecular field analysis (comfa) and the comparative molecular similarity indices analysis (comsia), the paper describes two three-dimensional quantitative structure-activity relationship (3d-qsar) models for the acute toxicity logec50 (15 min-ec50 in micromoll(-1)) of 56 phenylsulfonyl carboxylates on photobacterium phosphoreum. two models yield the leave-one-out cross-validated correlation coefficient q2 values of 0.823 and 0.713, and the conventional correlation coef ...	2003	14505717
purification and characterization of an antibacterial protein in the skin secretion of rockfish sebastes schlegeli.	an antibacterial protein in the skin secretion of rockfish (sebastes schlegeli) was purified by lectin affinity chromatography on con a-sepharose and gel filtration on tskgel g3000sw. the antibacterial protein featured the high molecular mass and selective action against gram-negative bacteria. the molecular mass of the protein was estimated to be approximately 150 kda in gel filtration and approximately 75 kda by sds-page, suggesting that it is dimeric. the antibacterial principle was an acidic ...	2003	14522599
[comparative study of microbial communities from cultured and natural population of the mussel mytilus trossulus in peter the great bay].	the 525 strains of heterotrophic bacteria isolated from natural and cultured populations of the mussel mytilus trossulus and the surrounding seawater were identified to a genus level on the basis of phenotypic analysis and the fatty acid composition of cell lipids. gram-negative isolates were dominated by six genera of the family enterobacteriaceae and by the genera pseudoalteromonas, vibrio, photobacterium, cytophaga/flavobacterium/bacteroides, pseudomonas, and moraxella, gram-positive isolates ...	2003	14526545
development of qsars for predicting the joint effects between cyanogenic toxicants and aldehydes.	quantitative structure-activity relationship (qsar) approaches are proposed in this study to predict the joint effects of mixture toxicity. the initial investigation studies the joint effects between cyanogenic toxicants and aldehydes to photobacterium phosphoreum. joint effects are found to result from the formation of a carbanion intermediate produced through the chemical interactions between cyanogenic toxicants and aldehydes. further research indicates that the formation of carbanion interme ...	2003	14565777
[effect of mycotoxin t-2 on bioluminescence intensity of bacteria].	the acute and chronic toxicity of t-2 was studied by bioluminescent method with the use of two strains of luminous bacteria--p. phosphorum sq3 u v. fischeri f1 as biological objects. it was shown that in acute experiments after 10 min incubation of bacteria in the presence of t-2 the bioluminescence inhibition on the 50% level was observed at the toxin concentration equal to 12 mg/ml. in chronic experiments such a level of bioluminescence inhibition was registered after 16 hours incubation at th ...	2003	14577159
bacterial influences on atlantic halibut hippoglossus hippoglossus yolk-sac larval survival and start-feed response.	a bacteria-free halibut larval rearing system was used to test 20 bacterial isolates, from british halibut hatcheries, for their toxicity towards halibut yolk-sac larvae under microbially controlled conditions. the isolates tested spanned a range of genera and species (pseudoalteromonas, halomonas marina, vibrio salmonicida-like, photobacterium phosphoreum and v. splendidus species). a pathogen of turbot, scophthalmus maximus, v. anguillarum 91079, and 2 isolates from adult halibut were also inc ...	2003	14598986
isolation and identification of photobacterium phosphoreum from an unexpected niche: migrating salmon.	six luminous bacteria were isolated from migrating salmon in the yukon river, alaska. all isolates were identified as photobacterium phosphoreum. previous studies suggest that p. phosphoreum is an exclusively marine bacterium, while our alaskan isolates are from salmon which migrated up to 1,228 km from the marine environment.	2003	14602659