Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| effects of mutation at position 285 of ralstonia pickettii t1 poly[(r)-3-hydroxybutyrate] depolymerase on its activities. | asn at position 285 (n285) in the catalytic domain of poly[(r)-3-hydroxybutyrate] (phb) depolymerase from ralstonia pickettii t1 most likely participates in the cleavage of ester bonds as revealed by our previous evolutionary engineering study using the error-prone polymerase chain reaction (pcr) method. to exhaustively examine the effects of mutations at that position, we conducted site-directed saturation mutagenesis at that position and the resultant mutant enzymes (n285x) were evaluated in p ... | 2014 | 24676749 |
| [outbreak with ralstonia pickettii caused by contaminated magnesium vials]. | in february 2013, 5 patients in an intensive care unit (icu) were found to have positive blood cultures with ralstonia pickettii within one week. because all patients got intravenous therapy, improper work of a staff member was suspected. some days later, a 6th patient was found with a positive blood culture of ralstonia pickettii in another department of the hospital. | 2014 | 24496893 |
| transcriptional repression of the poly(3-hydroxybutyrate) depolymerase in ralstonia pickettii t1 by a tetr-like gene. | ralstonia pickettii t1 secretes a poly(3-hydroxybutyrate) (phb) depolymerase (phaz) and a 3-hydroxybutyrate (3hb)-oligomer hydrolase, and extracellularly degrades phb to produce 3hb. however, it is not clear how the expression of phaz is regulated. in this study, the mechanism by which phaz expression is controlled in r. pickettii t1 was examined using a mutant made by the random insertion of a transposon, tn5. the mutant produced a larger amount of phaz than the wild type in nutrient broth or a ... | 2014 | 24146107 |
| ralstonia spp.: emerging global opportunistic pathogens. | the bacterial genus ralstonia (gram-negative non-fermenters) is becoming more prevalent in cases of infection with three bacterial species, ralstonia pickettii, ralstonia insidiosa and ralstonia mannitolilytica, making up all cases reported (in the literature) to date. these organisms are prevalent in many different types of water supplies (including hospital water supplies), being well adapted to survive in low-nutrient conditions. they have been shown to cause infections, sometimes serious, su ... | 2014 | 24057141 |
| real-time comparative evaluation of biomerieux vitek ms versus bruker microflex ms, two matrix-assisted laser desorption-ionization time-of-flight mass spectrometry systems, for identification of clinically significant bacteria. | matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (maldi-tof ms) recently became available for the identification of bacteria in routine diagnostic laboratories. it is rapid and cost-effective and likely to replace phenotypic identification. this study was undertaken to compare two maldi-tof ms-based, bruker microflex ms (bms) and vitek ms (vms) systems, for identification (id) of clinically significant bacterial isolates. clinically relevant broad diversity of bacteri ... | 2014 | 25433488 |
| the crystal structure of pyrimidine/thiamin biosynthesis precursor-like domain-containing protein cae31940 from proteobacterium bordetella bronchiseptica rb50, and evolutionary insight into the nmt1/thi5 family. | we report a 2.0 å structure of the cae31940 protein, a proteobacterial nmt1/thi5-like domain-containing protein. we also discuss the primary and tertiary structure similarity with its homologs. the highly conserved fggxmp motif was identified in cae31940, which corresponds to the gcccx motif located in the vicinity of the active center characteristic for thi5-like proteins found in yeast. this suggests that the fggxmp motif may be a unique hallmark of proteobacterial nmt1/thi5-like proteins. | 2014 | 24908050 |
| a brief review: the z-curve theory and its application in genome analysis. | in theoretical physics, there exist two basic mathematical approaches, algebraic and geometrical methods, which, in most cases, are complementary. in the area of genome sequence analysis, however, algebraic approaches have been widely used, while geometrical approaches have been less explored for a long time. the z-curve theory is a geometrical approach to genome analysis. the z-curve is a three-dimensional curve that represents a given dna sequence in the sense that each can be uniquely reconst ... | 2014 | 24822026 |
| f-/cl- selectivity in clcf-type f-/h+ antiporters. | many bacterial species protect themselves against environmental f(-) toxicity by exporting this anion from the cytoplasm via clc(f) f(-)/h(+) antiporters, a subclass of clc superfamily anion transporters. strong f(-) over cl(-) selectivity is biologically essential for these membrane proteins because cl(-) is orders of magnitude more abundant in the biosphere than f(-). sequence comparisons reveal differences between clc(f)s and canonical cl(-)-transporting clcs within regions that, in the canon ... | 2014 | 25070431 |
| isolation, identification and characterization of burkholderia pseudomallei from soil of coastal region of india. | melioidosis is an emerging infectious disease caused by a free living soil dwelling gram-negative bacterium burkholderia pseudomallei. the disease is endemic to most parts of southeast asia and northern australia and the organism has been isolated from moist soil and water. in india clinical cases are recently reported from the states of tamilnadu, kerala, karnataka, maharashtra, orissa, assam, west bengal, pondicherry and tripura. this study is aimed to confirm the prevalence of this important ... | 2014 | 25187882 |
| persistence of microbial communities including pseudomonas aeruginosa in a hospital environment: a potential health hazard. | the persistence of microbial communities and how they change in indoor environments is of immense interest to public health. moreover, hospital acquired infections are significant contributors to morbidity and mortality. evidence suggests that, in hospital environments agent transfer between surfaces causes healthcare associated infections in humans, and that surfaces are an important transmission route and may act as a reservoir for some of the pathogens.this study aimed to evaluate the diversi ... | 2014 | 24885173 |
| performance of mass spectrometric identification of bacteria and yeasts routinely isolated in a clinical microbiology laboratory using maldi-tof ms. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is an emerging technology newly applied to identifying bacterial and yeast strains. the aim of this study was to evaluate the clinical performance of the vitek(®) ms system in the identification of bacteria and yeast strains routinely isolated from clinical samples. | 2014 | 24822114 |
| microbial redox processes in deep subsurface environments and the potential application of (per)chlorate in oil reservoirs. | the ability of microorganisms to thrive under oxygen-free conditions in subsurface environments relies on the enzymatic reduction of oxidized elements, such as sulfate, ferric iron, or co2, coupled to the oxidation of inorganic or organic compounds. a broad phylogenetic and functional diversity of microorganisms from subsurface environments has been described using isolation-based and advanced molecular ecological techniques. the physiological groups reviewed here comprise iron-, manganese-, and ... | 2014 | 25225493 |
| evaluation of molecular methods to improve the detection of burkholderia pseudomallei in soil and water samples from laos. | burkholderia pseudomallei is the cause of melioidosis, a severe and potentially fatal disease of humans and animals. it is endemic in northern australia and southeast asia and is found in soil and surface water. the environmental distribution of b. pseudomallei worldwide and within countries where it is endemic, such as the lao people's democratic republic (laos), remains unclear. however, this knowledge is important to our understanding of the ecology and epidemiology of b. pseudomallei and to ... | 2015 | 25819969 |
| a duplex pcr assay for the detection of ralstonia solanacearum phylotype ii strains in musa spp. | banana wilt outbreaks that are attributable to moko disease-causing strains of the pathogen ralstonia solanacearum (rs) remain a social and economic burden for both multinational corporations and subsistence farmers. all known moko strains belong to the phylotype ii lineage, which has been previously recognized for its broad genetic basis. moko strains are paraphyletic and are distributed among seven related but distinct phylogenetic clusters (sequevars) that are potentially major threats to mus ... | 2015 | 25811378 |
| bacteria that travel: the quality of aircraft water. | the travelling population is increasing globally year on year. international tourist arrival figures reached 1087 million in 2013 and 1133 million in 2014; of which 53% and 54% respectively accounted for air transport. the water on board aircraft is sourced from surface or ground water; piped to a central filling point and distributed to each aircraft by water service vehicles at the home base or at the destination airport. the purpose of this study was to ascertain the microbial, chemical (ph; ... | 2015 | 26529000 |
| the influence of smoking on the peri-implant microbiome. | smokers are at high risk for 2 bacterially driven oral diseases: peri-implant mucositis and peri-implantitis. therefore, the purpose of this investigation was to use a deep-sequencing approach to identify the effect of smoking on the peri-implant microbiome in states of health and disease. peri-implant biofilm samples were collected from 80 partially edentulous subjects with peri-implant health, peri-implant mucositis, and peri-implantitis. bacterial dna was isolated and 16s ribsomal rna gene li ... | 2015 | 26124222 |
| the characterization of novel tissue microbiota using an optimized 16s metagenomic sequencing pipeline. | substantial progress in high-throughput metagenomic sequencing methodologies has enabled the characterisation of bacteria from various origins (for example gut and skin). however, the recently-discovered bacterial microbiota present within animal internal tissues has remained unexplored due to technical difficulties associated with these challenging samples. | 2015 | 26544955 |
| the legionella pneumophila siderophore legiobactin is a polycarboxylate that is identical in structure to rhizoferrin. | legionella pneumophila, the agent of legionnaires' disease, secretes a siderophore (legiobactin) that promotes bacterial infection of the lung. in past work, we determined that cytoplasmic lbta (from legiobactin gene a) promotes synthesis of legiobactin, inner membrane lbtb aids in export of the siderophore, and outer membrane lbtu and inner membrane lbtc help mediate ferrilegiobactin uptake and assimilation. however, the past studies examined legiobactin contained within bacterial culture super ... | 2015 | 26195554 |
| infectious causes of cholesteatoma and treatment of infected ossicles prior to reimplantation by hydrostatic high-pressure inactivation. | chronic inflammation, which is caused by recurrent infections, is one of the factors contributing to the pathogenesis of cholesteatoma. if reimplantation of autologous ossicles after a surgical intervention is intended, inactivation of planktonic bacteria and biofilms is desirable. high hydrostatic pressure treatment is a procedure, which has been used to inactivate cholesteatoma cells on ossicles. here we discuss the potential inactivating effect of high hydrostatic pressure on microbial pathog ... | 2015 | 25705686 |
| paenibacillus larvae-directed bacteriophage hb10c2 and its application in american foulbrood-affected honey bee larvae. | paenibacillus larvae is the causative agent of american foulbrood (afb), the most serious honey bee brood bacterial disease. we isolated and characterized p. larvae-directed bacteriophages and developed criteria for safe phage therapy. whole-genome analysis of a highly lytic virus of the family siphoviridae (hb10c2) provided a detailed safety profile and uncovered its lysogenic nature and a putative beta-lactamase-like protein. to rate its antagonistic activity against the pathogens targeted and ... | 2015 | 26048941 |
| microbial toluene removal in hypoxic model constructed wetlands occurs predominantly via the ring monooxygenation pathway. | in the present study, microbial toluene degradation in controlled constructed wetland model systems, planted fixed-bed reactors (pfrs), was queried with dna-based methods in combination with stable isotope fractionation analysis and characterization of toluene-degrading microbial isolates. two pfr replicates were operated with toluene as the sole external carbon and electron source for 2 years. the bulk redox conditions in these systems were hypoxic to anoxic. the autochthonous bacterial communi ... | 2015 | 26150458 |
| draft genome sequence of ralstonia sp. md27, a poly(3-hydroxybutyrate)-degrading bacterium, isolated from compost. | ralstonia sp. strain md27, a novel biopolymer-degrading betaproteobacterium, was isolated from compost samples. this organism has been shown to utilize the biopolymer poly(3-hydroxybutyrate) [p(3hb)] as a carbon source for growth. we report the draft genome sequence of md27 with an estimated total sequence length of 5.9 mb. | 2015 | 26450738 |
| validation and application of a real-time pcr protocol for the specific detection and quantification of clavibacter michiganensis subsp. sepedonicus in potato. | clavibacter michiganensis subsp. sepedonicus (cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. although several pcr assays have been developed for detection, they cannot afford specific detection of cms. therefore, in this study, a computational genome analysis was performed to compare the sequenced gen ... | 2015 | 26060431 |
| an sos regulon under control of a noncanonical lexa-binding motif in the betaproteobacteria. | the sos response is a transcriptional regulatory network governed by the lexa repressor that activates in response to dna damage. in the betaproteobacteria, lexa is known to target a palindromic sequence with the consensus sequence ctgt-n8-acag. we report the characterization of a lexa regulon in the iron-oxidizing betaproteobacterium sideroxydans lithotrophicus. in silico and in vitro analyses show that lexa targets six genes by recognizing a binding motif with the consensus sequence gaacgaacgt ... | 2015 | 25986903 |
| opportunistic pathogens and elements of the resistome that are common in bottled mineral water support the need for continuous surveillance. | several differences concerning bacterial species, opportunistic pathogens, elements of the resistome as well as variations concerning the cfu/ml counts were identified in some of the five most marketed bottled mineral water from araraquara city, são paulo, brazil. two out of five brands tested were confirmed as potential source of opportunistic pathogens, including mycobacterium gordonae, ralstonia picketti and burkholderia cepacia complex (bcc). a total of one hundred and six isolates were reco ... | 2015 | 25803794 |
| contribution of the bact/alert mb mycobacterium bottle to bloodstream infection surveillance in thailand: added yield for burkholderia pseudomallei. | community-acquired bloodstream infections cause substantial morbidity and mortality worldwide, but microbiology capacity and surveillance limitations have challenged good descriptions of pathogen distribution in many regions, including southeast asia. active surveillance for bloodstream infections has been conducted in two rural thailand provinces for >7 years. blood specimens were divided into two culture bottles, one optimized for aerobic growth (f bottle) and a second for enhanced growth of m ... | 2015 | 25588650 |
| sph3 is a glycoside hydrolase required for the biosynthesis of galactosaminogalactan in aspergillus fumigatus. | aspergillus fumigatus is the most virulent species within the aspergillus genus and causes invasive infections with high mortality rates. the exopolysaccharide galactosaminogalactan (gag) contributes to the virulence of a. fumigatus. a co-regulated five-gene cluster has been identified and proposed to encode the proteins required for gag biosynthesis. one of these genes, sph3, is predicted to encode a protein belonging to the spherulin 4 family, a protein family with no known function. construct ... | 2015 | 26342082 |
| the variability of the order burkholderiales representatives in the healthcare units. | the order burkholderiales became more abundant in the healthcare units since the late 1970s; it is especially dangerous for intensive care unit patients and patients with chronic lung diseases. the goal of this investigation was to reveal the real variability of the order burkholderiales representatives and to estimate their phylogenetic relationships. | 2015 | 26114111 |
| comparative metagenomics reveals impact of contaminants on groundwater microbiomes. | to understand patterns of geochemical cycling in pristine versus contaminated groundwater ecosystems, pristine shallow groundwater (fw301) and contaminated groundwater (fw106) samples from the oak ridge integrated field research center (or-ifrc) were sequenced and compared to each other to determine phylogenetic and metabolic difference between the communities. proteobacteria (e.g., burkholderia, pseudomonas) are the most abundant lineages in the pristine community, though a significant proporti ... | 2015 | 26583008 |
| identification of the mcpa and mcpm genes, encoding methyl-accepting proteins involved in amino acid and l-malate chemotaxis, and involvement of mcpm-mediated chemotaxis in plant infection by ralstonia pseudosolanacearum (formerly ralstonia solanacearum phylotypes i and iii). | sequence analysis has revealed the presence of 22 putative methyl-accepting chemotaxis protein (mcp) genes in the ralstonia pseudosolanacearum gmi1000 genome. pcr analysis and dna sequencing showed that the highly motile r. pseudosolanacearum strain ps29 possesses homologs of all 22 r. pseudosolanacearum gmi1000 mcp genes. we constructed a complete collection of single mcp gene deletion mutants of r. pseudosolanacearum ps29 by unmarked gene deletion. screening of the mutant collection revealed t ... | 2015 | 26276117 |
| new metabolic pathway for degradation of 2-nitrobenzoate by arthrobacter sp. spg. | arthrobacter sp. spg utilized 2-nitrobenzoate as its sole source of carbon and energy and degraded it with accumulation of stoichiometric amounts of nitrite ions. salicylate and catechol were detected as metabolites of the 2-nitrobenzoate degradation using high performance liquid chromatography and gas chromatography-mass spectrometry. enzyme activities for 2-nitrobenzoate-2-monooxygenase, salicylate hydroxylase, and catechol-1,2-dioxygenase were detected in the crude extracts of the 2-nitrobenz ... | 2015 | 26082768 |
| recent trends in control methods for bacterial wilt diseases caused by ralstonia solanacearum. | previous studies have described the development of control methods against bacterial wilt diseases caused by ralstonia solanacearum. this review focused on recent advances in control measures, such as biological, physical, chemical, cultural, and integral measures, as well as biocontrol efficacy and suppression mechanisms. biological control agents (bcas) have been dominated by bacteria (90%) and fungi (10%). avirulent strains of r. solanacearum, pseudomonas spp., bacillus spp., and streptomyces ... | 2015 | 25762345 |
| extracellular lipase and protease production from a model drinking water bacterial community is functionally robust to absence of individual members. | bacteria secrete enzymes into the extracellular space to hydrolyze macromolecules into constituents that can be imported for microbial nutrition. in bacterial communities, these enzymes and their resultant products can be modeled as community property. our goal was to investigate the impact of individual community member absence on the resulting community production of exoenzymes (extracellular enzymes) involved in lipid and protein hydrolysis. our model community contained nine bacteria isolate ... | 2015 | 26599415 |
| discrepancy in maldi-tof ms identification of uncommon gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis. | early identification of microbial organisms from respiratory secretions of patients with cystic fibrosis (cf) is important to guide therapeutic decisions. the objective was to compare the accuracy of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) relative to the conventional phenotypic method in identifying common bacterial isolates, including nonfermenting gram-negative bacteria, in a cohort of patients with cf. | 2015 | 25995646 |
| phenotypic and phylogenetic identification of coliform bacteria obtained using 12 coliform methods approved by the u.s. environmental protection agency. | the current definition of coliform bacteria is method dependent, and when different culture-based methods are used, discrepancies in results can occur and affect the accuracy of identification of true coliforms. this study used an alternative approach to the identification of true coliforms by combining the phenotypic traits of the coliform isolates and the phylogenetic affiliation of 16s rrna gene sequences with the use of lacz and uida genes. a collection of 1,404 isolates detected by 12 u.s. ... | 2015 | 26116679 |
| genome sequence of a carbapenem-resistant strain of ralstonia mannitolilytica. | ralstonia mannitolilytica, a gram-negative aerobic bacterium, is an opportunistic human pathogen that is becoming more common in cases of nosocomial infections. we report for the first time the whole-genome sequence analysis of r. mannitolilytica strain mry14-0246, which carries the intrinsic oxa-443/oxa-22-like and oxa-444/oxa-60-like β-lactamase genes and is resistant to meropenem. | 2015 | 25953190 |
| the effects of silver nanoparticles on intact wastewater biofilms. | silver nanoparticles (ag-nps) have strong antibacterial properties, which may adversely affect biological wastewater treatment processes. to determine the overall effect, intact biofilm samples were collected from the rotating biological contactor at the local wastewater treatment plant and treated with 200 mg ag/l ag-nps for 24 h. the biofilm uptake of ag-nps was monitored with transmission electron microscopy. forty-five minutes after ag-np application, ag-nps were seen in the biofilm extracel ... | 2015 | 26217316 |
| genomic characterization of burkholderia pseudomallei isolates selected for medical countermeasures testing: comparative genomics associated with differential virulence. | burkholderia pseudomallei is the causative agent of melioidosis and a potential bioterrorism agent. in the development of medical countermeasures against b. pseudomallei infection, the us food and drug administration (fda) animal rule recommends using well-characterized strains in animal challenge studies. in this study, whole genome sequence data were generated for 6 b. pseudomallei isolates previously identified as candidates for animal challenge studies; an additional 5 isolates were sequence ... | 2015 | 25803742 |
| evaluation of antioxidant, free radical scavenging, and antimicrobial activity of quercus incana roxb. | considering the indigenous utilization of quercus incana roxb., the present study deals with the investigation of antioxidant, free radical scavenging activity, total phenolic content, and antimicrobial activity of q. incana roxb. in vitro antioxidant activity of the plant fractions were determined by 1,1-diphenyl-2-picrylhydrazyl and nitric oxide scavenging method. total phenolic contents were determined by gallic acid equivalent and antimicrobial activities were determined by agar well diffusi ... | 2015 | 26635607 |
| bacteria may have multiple replication origins. | 2015 | 25941523 | |
| effect of exclusive enteral nutrition on the microbiota of children with newly diagnosed crohn's disease. | exclusive enteral nutrition (een) is commonly used to treat pediatric crohn's disease (cd). meta-analysis of pediatric studies that have compared the effect of een with other treatments have shown that een induces remission in up to 80-85% of patients. we aimed to gain a comprehensive understanding of the effect of een on the microbiota of cd patients. | 2015 | 25588524 |
| native valve endocarditis due to ralstonia pickettii: a case report and literature review. | ralstonia pickettii is a rare pathogen and even more rare in healthy individuals. here we report a case of r. pickettii bacteremia leading to aortic valve abscess and complete heart block. to our knowledge this is the first case report of ralstonia species causing infective endocarditis with perivalvular abscess. | 2015 | 25648998 |
| genome sequence analysis of the naphthenic acid degrading and metal resistant bacterium cupriavidus gilardii cr3. | cupriavidus sp. are generally heavy metal tolerant bacteria with the ability to degrade a variety of aromatic hydrocarbon compounds, although the degradation pathways and substrate versatilities remain largely unknown. here we studied the bacterium cupriavidus gilardii strain cr3, which was isolated from a natural asphalt deposit, and which was shown to utilize naphthenic acids as a sole carbon source. genome sequencing of c. gilardii cr3 was carried out to elucidate possible mechanisms for the ... | 2015 | 26301592 |
| an outbreak of ralstonia pickettii endophthalmitis following intravitreal methotrexate injection. | to report an outbreak of endophthalmitis in three eyes of two patients following intravitreal methotrexate, caused by ralstonia pickettii. | 2015 | 26150690 |
| the genome and methylome of a beetle with complex social behavior, nicrophorus vespilloides (coleoptera: silphidae). | testing for conserved and novel mechanisms underlying phenotypic evolution requires a diversity of genomes available for comparison spanning multiple independent lineages. for example, complex social behavior in insects has been investigated primarily with eusocial lineages, nearly all of which are hymenoptera. if conserved genomic influences on sociality do exist, we need data from a wider range of taxa that also vary in their levels of sociality. here, we present the assembled and annotated ge ... | 2015 | 26454014 |
| high-flow nasal cannula therapy for obstructive sleep apnea in children. | over the last decade, high-flow nasal cannula (hfnc) therapy has become an increasingly important and popular mode of noninvasive respiratory support. hfnc facilitates delivery of humidified and heated oxygen at a high flow rate and generates positive airway pressure. | 2015 | 26094930 |
| genetic determinants of heat resistance in escherichia coli. | escherichia coli aw1.7 is a heat resistant food isolate and the occurrence of pathogenic strains with comparable heat resistance may pose a risk to food safety. to identify the genetic determinants of heat resistance, 29 strains of e. coli that differed in their of heat resistance were analyzed by comparative genomics. strains were classified as highly heat resistant strains, exhibiting a d60-value of more than 6 min; moderately heat resistant strains, exhibiting a d60-value of more than 1 min; ... | 2015 | 26441869 |
| fatal suppurative inflammation in daurian pikas (ochotona dauurica) naturally infected with castellaniella ginsengisoli-like bacteria. | fifteen daurian pika (ochotona dauurica) were introduced to a zoological collection in december 2011 as founders for a planned breeding colony. despite breeding success, the colony shrunk over 37 mo to 11 animals. mortality in 11 of 46 deceased animals, including wild-caught "founders" and captive-born offspring, was associated with a suppurative inflammation and abscess formation of the spleen, skin, peripheral and internal lymph nodes, liver, lungs, kidney, or a combination of organs. gram-neg ... | 2015 | 26352961 |
| molecular weaponry: diverse effectors delivered by the type vi secretion system. | the type vi secretion system is a widespread bacterial nanomachine, used to deliver toxins directly into eukaryotic or prokaryotic target cells. these secreted toxins, or effectors, act on diverse cellular targets, and their action provides the attacking bacterial cell with a significant fitness advantage, either against rival bacteria or eukaryotic host organisms. in this review, we discuss the delivery of diverse effectors by the type vi secretion system, the modes of action of the so-called ' ... | 2015 | 26432982 |
| 8th annual symposium on self-monitoring of blood glucose (smbg): april 16-18, 2015, republic of malta. | international experts in the fields of diabetes, diabetes technology, endocrinology, mobile health, sport science, and regulatory issues gathered for the 8(th) annual symposium on self-monitoring of blood glucose (smbg) with a focus on personalized diabetes management. the aim of this meeting was to facilitate new collaborations and research projects to improve the lives of people with diabetes. the 2015 meeting comprised a comprehensive scientific program, parallel interactive workshops, and tw ... | 2015 | 26496678 |
| intrinsic carbapenem-hydrolyzing oxacillinases from members of the genus pandoraea. | we analyzed the oxacillinases of isolates of six different species of pandoraea, a genus that colonizes the respiratory tract of cystic fibrosis patients. the isolates produced carbapenem-hydrolyzing enzymes causing elevated mics for amoxicillin, piperacillin, meropenem, and imipenem when expressed in an escherichia coli host strain. sequencing revealed nine new oxacillinases (oxa-151 to oxa-159) with a high degree of identity among isolates of the same species; however, they had much lower inte ... | 2015 | 26349828 |
| rpa, an extracellular protease similar to the metalloprotease of serralysin family, is required for pathogenicity of ralstonia pickettii. | to investigate the biochemical and functional properties of an extracellular protease, rpa, in ralstonia pickettii wp1 isolated from water supply systems. | 2015 | 26184602 |
| assessment of copper and zinc salts as selectors of antibiotic resistance in gram-negative bacteria. | some metals are nowadays considered environmental pollutants. although some, like cu and zn, are essential for microorganisms, at high concentrations they can be toxic or exert selective pressures on bacteria. this study aimed to assess the potential of cu or zn as selectors of specific bacterial populations thriving in wastewater. populations of escherichia coli recovered on metal-free and metal-supplemented culture medium were compared based on antibiotic resistance phenotype and other traits. ... | 2015 | 26057541 |
| outbreak of ralstonia pickettii bacteremia caused by contaminated saline solution in taiwan. | 2016 | 27424301 | |
| pcr-based assay for the rapid and precise distinction of pseudomonas aeruginosa from other pseudomonas species recovered from burns patients. | pseudomonas aeruginosa is an important lifethreatening nosocomial pathogen which plays a prominent role in wound infections in burns patients. we designed this study to identify the isolates of p. aeruginosa recovered from burns patients at the genus and species levels by means of primers targeting opri and oprl genes. | 2016 | 27582633 |
| [fulminant sepsis due to ralstonia pickettii]. | 2016 | 27262571 | |
| ralstonia pickettii bacteremia in hemodialysis patients: a report of two cases. | ralstonia pickettii is a low-virulence gram-negative bacillus that may be associated with infections related to health care and may cause bacteremia. ralstonia pickettii bacteremia is uncommon but is related to the contamination of medical products, mainly in immunodepressed patients. we present two cases of patients on chronic hemodialysis with ralstonia pickettii bacteremia linked to contamination of the dialysis water. similar cases have been published with links to intravenous fluid administ ... | 2016 | 27410414 |
| a novel ammonia-oxidizing archaeon from wastewater treatment plant: its enrichment, physiological and genomic characteristics. | ammonia-oxidizing archaea (aoa) are recently found to participate in the ammonia removal processes in wastewater treatment plants (wwtps), similar to their bacterial counterparts. however, due to lack of cultivated aoa strains from wwtps, their functions and contributions in these systems remain unclear. here we report a novel aoa strain sat1 enriched from activated sludge, with its physiological and genomic characteristics investigated. the maximal 16s rrna gene similarity between sat1 and othe ... | 2016 | 27030530 |
| draft genome sequences of two ralstonia pickettii strains with different aminoglycoside resistance phenotypes. | the genomes of two ralstonia pickettii strains (h2cu2 and h2cu5), isolated from hospital effluent in a selective medium containing cuso4, were sequenced. they presented mics of >256 and 6 µg/ml for the aminoglycoside gentamicin, respectively. the 5.2-mb draft genomes have 40 contigs for strain h2cu2 and 113 for h2cu5. | 2016 | 27834709 |
| genome sequences of ralstonia insidiosa type strain atcc 49129 and strain fc1138, a strong biofilm producer isolated from a fresh-cut produce-processing plant. | ralstonia insidiosa is an opportunistic pathogen and a strong biofilm producer. here, we present the complete genome sequences of r. insidiosa fc1138 and atcc 49129. both strains have two circular chromosomes of approximately 3.9 and 1.9 mb and a 50-kb plasmid. atcc 49129 also possesses a megaplasmid of approximately 318 kb. | 2016 | 27540070 |
| aerobic toluene degraders in the rhizosphere of a constructed wetland model show diurnal polyhydroxyalkanoate metabolism. | constructed wetlands (cws) are successfully applied for the treatment of waters contaminated with aromatic compounds. in these systems, plants provide oxygen and root exudates to the rhizosphere and thereby stimulate microbial degradation processes. root exudation of oxygen and organic compounds depends on photosynthetic activity and thus may show day-night fluctuations. while diurnal changes in cw effluent composition have been observed, information on respective fluctuations of bacterial activ ... | 2016 | 27129963 |
| a wide spectrum of fastidious and ampicillin-susceptible bacteria dominate in animal-caused wounds. | the main purpose of this study was to assess the actual occurrence of gram-negative oxidase-positive bacteria (gnop) in human wounds caused by animals, mostly cat and dog bites and scratches, and with signs of infection. we report a prospective series of 92 wound samples. routine culturing was combined with a procedure optimised for fastidious gnop. all gnop isolates were identified by 16s rdna sequencing to the species level. we observed a more prominent role of gnop, including at least 30 spec ... | 2016 | 27197725 |
| substrate and cofactor range differences of two cysteine dioxygenases from ralstonia eutropha h16. | cysteine dioxygenases (cdos), which catalyze the sulfoxidation of cysteine to cysteine sulfinic acid (csa), have been extensively studied in eukaryotes because of their roles in several diseases. in contrast, only a few prokaryotic enzymes of this type have been investigated. in ralstonia eutropha h16, two cdo homologues (cdoa and cdob) have been identified previously. in vivo studies showed that escherichia coli cells expressing cdoa could convert 3-mercaptopropionate (3mp) to 3-sulfinopropiona ... | 2016 | 26590284 |
| to: ralstonia pickettii bacteremia in hemodialysis patients: a report of two cases. | 2016 | 28099646 | |
| data from computational analysis of the peptide linkers in the mocr bacterial transcriptional regulators. | detailed data from statistical analyses of the structural properties of the inter-domain linker peptides of the bacterial regulators of the family mocr are herein reported. mocr regulators are a recently discovered subfamily of bacterial regulators possessing an n-terminal domain, 60 residue long on average, folded as the winged-helix-turn-helix architecture responsible for dna recognition and binding, and a large c-terminal domain (350 residue on average) that belongs to the fold type-i pyridox ... | 2016 | 27668276 |
| the complete sequences and ecological roles of two incp-1β plasmids, phb44 and pbs64, isolated from the mycosphere of laccaria proxima. | two novel plasmids, coined phb44 and pbs64, were recently found in variovorax paradoxus strains hb44 and bs64 isolated from the mycosphere of laccaria proxima, on two different sampling occasions. we here describe the full sequences of phb44 and pbs64 and establish their evolutionary placement and ecological function. both plasmids, unique for mycospheric v. paradoxus, were around 58 kb in size. they possessed, in a very similar fashion, three main plasmid backbone regions, which were predicted ... | 2016 | 27445994 |
| metagenomic analysis of medicinal cannabis samples; pathogenic bacteria, toxigenic fungi, and beneficial microbes grow in culture-based yeast and mold tests. | background: the presence of bacteria and fungi in medicinal or recreational cannabis poses a potential threat to consumers if those microbes include pathogenic or toxigenic species. this study evaluated two widely used culture-based platforms for total yeast and mold (tym) testing marketed by 3m corporation and biomérieux, in comparison with a quantitative pcr (qpcr) approach marketed by medicinal genomics corporation. methods: a set of 15 medicinal cannabis samples were analyzed using 3m and bi ... | 2016 | 27853518 |
| degradation pathways of 2- and 4-nitrobenzoates in cupriavidus sp. strain st-14 and construction of a recombinant strain, st-14::3nba, capable of degrading 3-nitrobenzoate. | strain st-14, characterized as a member of the genus cupriavidus, was capable of utilizing 2- and 4-nitrobenzoates individually as sole sources of carbon and energy. biochemical studies revealed the assimilation of 2- and 4-nitrobenzoates via 3-hydroxyanthranilate and protocatechuate, respectively. screening of a genomic fosmid library of strain st-14 constructed in escherichia coli identified two gene clusters, onb and pob-pca, to be responsible for the complete degradation of 2-nitrobenzoate a ... | 2016 | 27208126 |
| comparison of cultures and 16s rrna sequencing for identification of bacteria in two-stage revision arthroplasties: preliminary report. | the use of a prefabricated spacer in two-stage revision arthroplasty remains one of the few surgery strategies for infected-joint arthroplasty treatment, despite the many unidentified microorganisms in the infected joint replacements reported in some recent studies. the aim of this prospective survey was to investigate if the sonication followed by polymerase chain reaction (pcr) can improve bacterial identification on the surfaces of prefabricated spacers and if the systemic laboratory mediator ... | 2016 | 27015812 |
| bacterial communities associated with atherosclerotic plaques from russian individuals with atherosclerosis. | atherosclerosis is considered a chronic disease of the arterial wall and is the major cause of severe disease and death among individuals all over the world. some recent studies have established the presence of bacteria in atherosclerotic plaque samples and suggested their possible contribution to the development of cardiovascular disease. the main objective of this preliminary pilot study was to better understand the bacterial diversity and abundance in human atherosclerotic plaques derived fro ... | 2016 | 27736997 |
| complete genome sequence of the potato pathogen ralstonia solanacearum uy031. | ralstonia solanacearum is the causative agent of bacterial wilt of potato. ralstonia solanacearum strain uy031 belongs to the american phylotype iib, sequevar 1, also classified as race 3 biovar 2. here we report the completely sequenced genome of this strain, the first complete genome for phylotype iib, sequevar 1, and the fourth for the r. solanacearum species complex. in addition to standard genome annotation, we have carried out a curated annotation of type iii effector genes, an important p ... | 2016 | 26779304 |
| a comparison of tissue versus swab culturing of infected diabetic foot wounds. | objective. to compare the efficacy of swabbing versus tissue biopsy for microbiological diagnosis of diabetic foot infection. methods. this was a prospective trial. fifty-six patients with diabetic foot infection were divided into the following 3 groups according to the pedis grading system: grade 2 (n = 10), grade 3 (n = 29), and grade 4 (n = 17). two specimens were collected from each wound for microbial culturing after debridement, including a superficial swab and a deep tissue punch biopsy s ... | 2016 | 27123004 |
| site-directed mutagenesis from arg195 to his of a microalgal putatively chloroplastidial glycerol-3-phosphate acyltransferase causes an increase in phospholipid levels in yeast. | to analyze the contribution of glycerol-3-phosphate acyltransferase (gpat) to the first acylation of glycerol-3-phosphate (g-3-p), the present study focused on a functional analysis of the gpat gene from lobosphaera incisa (designated as ligpat). a full-length cdna of ligpat consisting of a 1,305-bp orf, a 1,652-bp 5'-utr, and a 354-bp 3'-utr, was cloned. the orf encoded a 434-amino acid peptide, of which 63 residues at the n-terminus defined a chloroplast transit peptide. multiple sequence alig ... | 2016 | 27014309 |
| prophylactically decontaminating human islet product for safe clinical application: effective and potent method. | transplanting pancreatic islets into recipients must be safe and effective to treat type 1 diabetes. islet quality and quantity are important, however, the final product must also be free from microbial contamination and low endotoxin levels. | 2016 | 26894230 |
| analysis of bacterial communities associated with potting media. | potting media are commonly used by growers in different parts of the world for potted plants, raising seedlings and for improving soil characteristics. this study was conducted to characterize bacterial communities occurring in 13 commercial potting media products originating from seven countries. | 2016 | 26844021 |
| in situ protein-sip highlights burkholderiaceae as key players degrading toluene by para ring hydroxylation in a constructed wetland model. | in constructed wetlands, organic pollutants are mainly degraded via microbial processes. helophytes, plants that are commonly used in these systems, provide oxygen and root exudates to the rhizosphere, stimulating microbial degradation. while the treatment performance of constructed wetlands can be remarkable, a mechanistic understanding of microbial degradation processes in the rhizosphere is still limited. we investigated microbial toluene removal in a constructed wetland model system combinin ... | 2016 | 26616584 |
| bioaugmentation: an emerging strategy of industrial wastewater treatment for reuse and discharge. | a promising long-term and sustainable solution to the growing scarcity of water worldwide is to recycle and reuse wastewater. in wastewater treatment plants, the biodegradation of contaminants or pollutants by harnessing microorganisms present in activated sludge is one of the most important strategies to remove organic contaminants from wastewater. however, this approach has limitations because many pollutants are not efficiently eliminated. to counterbalance the limitations, bioaugmentation ha ... | 2016 | 27571089 |
| marine and giant viruses as indicators of a marine microbial community in a riverine system. | viral communities are important for ecosystem function as they are involved in critical biogeochemical cycles and controlling host abundance. this study investigates riverine viral communities around a small rural town that influences local water inputs. myoviridae, siphoviridae, phycodnaviridae, mimiviridae, herpesviridae, and podoviridae were the most abundant families. viral species upstream and downstream of the town were similar, with synechoccocus phage, salinus, prochlorococcus phage, mim ... | 2016 | 27506856 |
| optimized use of the maldi biotyper system and the filmarray bcid panel for direct identification of microbial pathogens from positive blood cultures. | despite the current reliance on blood cultures (bcs), the diagnosis of bloodstream infections (bsis) can be sped up using new technologies performed directly on positive bc bottles. two methods (the maldi biotyper system and filmarray blood culture identification [bcid] panel) are potentially applicable. in this study, we performed a large-scale clinical evaluation (1,585 microorganisms from 1,394 bsi episodes) on the combined use of the maldi biotyper and filmarray bcid panel compared to a refe ... | 2016 | 26677254 |
| complete genome sequence analysis of pandoraea pnomenusa type strain dsm 16536(t) isolated from a cystic fibrosis patient. | 2016 | 26903988 | |
| clinical relevance of molecular identification of microorganisms and detection of antimicrobial resistance genes in bloodstream infections of paediatric cancer patients. | bloodstream infections (bsis) are the major cause of mortality in cancer patients. molecular techniques are used for rapid diagnosis of bsi, allowing early therapy and improving survival. we aimed to establish whether real-time quantitative polymerase chain reaction (qpcr) could improve early diagnosis and therapy in paediatric cancer patients, and describe the predominant pathogens of bsi and their antimicrobial susceptibility. | 2016 | 27585633 |
| members of the genus burkholderia: good and bad guys. | in the 1990s several biocontrol agents on that contained burkholderia strains were registered by the united states environmental protection agency (epa). after risk assessment these products were withdrawn from the market and a moratorium was placed on the registration of burkholderia-containing products, as these strains may pose a risk to human health. however, over the past few years the number of novel burkholderia species that exhibit plant-beneficial properties and are normally not isolate ... | 2016 | 27303639 |
| comprehensive laboratory evaluation of a highly specific lateral flow assay for the presumptive identification of bacillus anthracis spores in suspicious white powders and environmental samples. | we conducted a comprehensive, multiphase laboratory evaluation of the anthrax biothreat alert(®) test strip, a lateral flow immunoassay (lfa) for the rapid detection of bacillus anthracis spores. the study, conducted at 2 sites, evaluated this assay for the detection of spores from the ames and sterne strains of b. anthracis, as well as those from an additional 22 strains. phylogenetic near neighbors, environmental background organisms, white powders, and environmental samples were also tested. ... | 2016 | 27661796 |
| redox-coupled structural changes in nitrite reductase revealed by serial femtosecond and microfocus crystallography. | serial femtosecond crystallography (sfx) has enabled the damage-free structural determination of metalloenzymes and filled the gaps of our knowledge between crystallographic and spectroscopic data. crystallographers, however, scarcely know whether the rising technique provides truly new structural insights into mechanisms of metalloenzymes partly because of limited resolutions. copper nitrite reductase (cunir), which converts nitrite to nitric oxide in denitrification, has been extensively studi ... | 2016 | 26769972 |
| kinetic mechanism of the dechlorinating flavin-dependent monooxygenase hada. | the accumulation of chlorophenols (cps) in the environment, due to their wide use as agrochemicals, has become a serious environmental problem. these organic halides can be degraded by aerobic microorganisms, where the initial steps of various biodegradation pathways include an oxidative dechlorinating process in which chloride is replaced by a hydroxyl substituent. harnessing these dechlorinating processes could provide an opportunity for environmental remediation, but detailed catalytic mechan ... | 2017 | 28159841 |
| an outbreak of ralstonia pickettii bloodstream infection associated with an intrinsically contaminated normal saline solution. | objective ralstonia pickettii has caused contamination of pharmaceutical solutions in many countries, resulting in healthcare infections or outbreak events. we determined the source of the outbreak of r. pickettii bloodstream infection (bsi). methods this study was conducted in a 3,000-bed tertiary referral medical center in taiwan with >8,500 admissions during may 2015. patients had been treated in the injection room or chemotherapy room at outpatient departments, emergency department, or hospi ... | 2017 | 28115025 |
| reply to: ralstonia pickettii bacteremia in hemodialysis patients: a report of two cases. | 2017 | 28099647 |