Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| human cytomegalovirus capsid assembly protein precursor (pul80.5) interacts with itself and with the major capsid protein (pul86) through two different domains. | we have used the yeast gal4 two-hybrid system to examine interactions between the human cytomegalovirus (hcmv) major capsid protein (mcp, encoded by ul86) and the precursor assembly protein (pap, encoded by ul80.5 and cleaved at its carboxyl end to yield ap) and found that (i) the pap interacts with the mcp through residues located within the carboxy-terminal 21 amino acids of the pap, called the carboxyl conserved domain (ccd); (ii) the pap interacts with itself through a separate region, calle ... | 1997 | 8985337 |
| detection and quantification of human cytomegalovirus (cmv) as a marker for development of cmv disease and survival in patients with aids. | 1997 | 11327439 | |
| three t-cell epitopes within the c-terminal 265 amino acids of the matrix protein pp65 of human cytomegalovirus recognized by human lymphocytes. | although a t-cell response in human cytomegalovirus (hcmv)-immune individuals exists against the most abundantly expressed protein pp65 of the virus matrix, less is known about the determinants that evoke this response. the aim of the study was to identify regions within hcmv pp65 (ppul83) that contain sequences for the cellular immune response by the use of three recombinant overlapping beta-galactosidase pp65 fusion proteins (c74, c35, and c47), covering the c-terminal 265 amino acids of the e ... | 1997 | 9131460 |
| use of polymerase chain reaction to provide prognostic information on human cytomegalovirus disease after liver transplantation. | sixty-four consecutive liver transplant patients receiving 76 organs have been monitored for human cytomegalovirus (hcmv) in blood and urine posttransplantation using a polymerase chain reaction (pcr) assay that amplifies a 149 base pair fragment of the glycoprotein b gene. six hundred and twenty-six blood and 310 urine samples were analysed during surveillance. thirty-two patients had cmv infection (50%), 12 of whim progressed to hcmv disease. detection of hcmv in either blood or urine was sign ... | 1997 | 9139077 |
| the human cytomegalovirus ul97 protein is phosphorylated and a component of virions. | the expression of the human cytomegalovirus (hcmv) ul97 open reading frame in infected or transfected cells in the presence of the antiherpes compound ganciclovir (gcv) results in the intracellular phosphorylation of gcv. there are conventional kinase domains within the ul97-encoded protein (pul97). however, the role of pul97 in the hcmv replication cycle, and the mechanism by which it causes phosphorylation of gcv, are currently unknown. herein, the biosynthesis and biogenesis of pul97 was stud ... | 1997 | 9143304 |
| cytomegalovirus immediate early genes upregulate interleukin-6 gene expression. | the immediate early genes (ie) of human cytomegalovirus (cmv) can be expressed in monocytic cells and are known to regulate viral and cellular genes. interleukin-6 (il-6) plays a central role in numerous inflammatory and immune processes. interleukin-6 levels are increased in lung transplant patients clinically diagnosed with cmv pneumonitis. the regulation of il-6 is dependent on various stimuli that include lipopolysaccharide (lps), viruses, and other cytokines. these studies examined the abil ... | 1997 | 9154298 |
| differential effects of hsv-1 and hcmv infection on adhesion molecule expression on human corneal keratocytes. | previous studies have shown that keratoplasty buttons obtained at surgery from patients with herpes simplex virus-1 (hsv-1) keratitis have elevated localized expression of the adhesion molecule icam-1, which plays a critical role in the initiation and amplification of an immune response. we performed studies to determine whether changes in expression of icam-1 and hla class i are direct effects of productive infection of human corneal fibroblasts with hsv-1. | 1997 | 9154389 |
| alteration in the expression of endothelial cell integrin receptors alpha 5 beta 1 and alpha 2 beta 1 and alpha 6 beta 1 after in vitro infection with a clinical isolate of human cytomegalovirus. | human cytomegalovirus infection of human umbilical vein endothelial cells reduces the ability of these cells to bind to fibronectin, collagen type iv and laminin. this suppression requires active virus, since uv-inactivated virus did not alter the binding ability of these cells to adhere to fibronectin, collagen type iv, and laminin. in an attempt to elucidate the molecular mechanism of this altered interaction, the surface expression of alpha 5 beta 1, alpha 2 beta 1, alpha 3 beta 1, and alpha ... | 1997 | 9155877 |
| human cytomegalovirus does not induce human leukocyte antigen class ii expression on arterial endothelial cells. | human cytomegalovirus (cmv) has been associated with allograft rejection and, in particular, with transplant-associated arteriosclerosis. however, the role cmv plays in the development of transplant-associated arteriosclerosis remains unclear. cmv can infect the endothelium, the interface between allograft tissue and the host immune cells, but the direct induction of endothelial human leukocyte antigen (hla) class ii by cmv remains controversial. our previous studies with venous endothelial cell ... | 1997 | 9158037 |
| human t-cell lymphotropic virus tax and epstein-barr virus dna in peripheral blood of multiple sclerosis patients during acute attack. | a study was performed to determine whether persistent or latent viruses are reactivated during the acute attack in relapsing remitting multiple sclerosis (ms). | 1997 | 9174643 |
| evaluation of retinal toxicity and efficacy of anti-cytomegalovirus and anti-herpes simplex virus antiviral phosphorothioate oligonucleotides isis 2922 and isis 4015. | retinal toxicity of isis 2922 and isis 4015, phosphorothioate oligonucleotides complementary to human cytomegalovirus (cmv) and herpes simplex virus (hsv) rna, were evaluated. the intravitreal concentration of isis 2922 found not to cause permanent toxic changes in the rabbit retina was 10 microm and in the pig retina, 5 microm. the 3 microm concentration was associated with a transient inflammatory response, and 1 microm caused no retinal toxicity or inflammation. isis 4015 showed very mild tox ... | 1997 | 9180168 |
| the human cytomegalovirus ul55 (gb) and ul75 (gh) glycoprotein ligands initiate the rapid activation of sp1 and nf-kappab during infection. | the cellular transcription factors sp1 and nf-kappab were upregulated shortly after the binding of purified live or uv-inactivated human cytomegalovirus (hcmv) to the cell surface. the rapid time frame of transcription factor induction is similar to that seen in other systems in which cellular factors are induced following receptor-ligand engagement. this similarity suggested that a cellular receptor-viral ligand interaction might be involved in sp1 and nf-kappab activation during the earliest s ... | 1997 | 9188570 |
| probing for cerebrospinal fluid antibody specificities by a panel of random peptide libraries. | in a state of health the central nervous system is nearly devoid of macrophages, t and b cells. however, such cells are required to initiate the inflammation associated with multiple sclerosis (ms). an important issue in understanding the pathogenesis of such conditions is to fully define the t and b cell specificities. using random peptide libraries, we have analysed the cerebrospinal fluid antibody specificities within one oligoclonal "band" from a patient with ms. the selected peptides from a ... | 1997 | 9189009 |
| transgene expression of plasmid dnas directed by viral or neural promoters in the rat brain. | the use of circular plasmid dna may be an alternative method for the transfer of genes into the brain and is presumably easier to use than other vectors, such as viruses or genetically engineered cells. the effectiveness and time course of the expression of a reporter gene (lacz), directed by appropriate promoters, was studied after stereotaxic injection of naked plasmid dnas into the rat thalamus, cortex or cerebellum. the efficiencies of three different promoters, the human cytomegalovirus (hc ... | 1997 | 9191082 |
| human cytomegalovirus immediate early interaction with host nuclear structures: definition of an immediate transcript environment. | the development of an induced transcript environment was investigated at the supramolecular level through comparative localization of the human cytomegalovirus immediate early (ie) transcripts and specific nuclear domains shortly after infection. compact aggregates of ie transcripts form only adjacent to nuclear domain 10 (nd10), and the viral protein ie86 accumulates exclusively juxtaposed to the subpopulation of nd10 with transcripts. the stream of transcripts is funneled from nd10 into the sp ... | 1997 | 9214377 |
| the role of atf in regulating the human cytomegalovirus dna polymerase (ul54) promoter during viral infection. | previous analysis of the human cytomegalovirus (hcmv) dna polymerase (ul54) early gene promoter demonstrated that transcriptional activation of this gene is dependent upon the interaction of cellular transcription factors with viral transactivators (j. a. kerry, m. a. priddy, t. y. jervey, c. p. kohler, t. l. staley, c. d. vanson, t. r. jones, a. c. iskenderian, d. g. anders, and r. m. stenberg, j. virol. 70:373-382, 1996). a sequence element, ir1, was shown to be the primary regulatory element ... | 1997 | 9032345 |
| comparison of human cytomegalovirus (hcmv) protease sequences among laboratory strains and seven clinical isolates. | the nucleotide sequence of the human cytomegalovirus (hcmv) protease gene from two laboratory strains and seven clinical isolates, both ganciclovir-sensitive and -resistant, was examined to determine the genetic variability of the hcmv protease catalytic domain and to identify changes that may alter the efficacy of designed protease inhibitors. the towne strain varied from ad169 at 12 nucleotides and led to one amino acid change at position 12 (ala to thr). the clinical isolates had amino acid s ... | 1997 | 9037377 |
| polyanion inhibitors of human immunodeficiency virus and other viruses. 5. telomerized anionic surfactants derived from amino acids. | omega-acryloyl anionic surfactants, whose polar heads are derived from amino acids, have been telomerized to prepare polyanions of a predetermined molecular weight. the main goal of this study was to verify whether the antiviral activity is influenced by the degree of polymerization of the polyanions. the oligomeric polyanions were evaluated for their activity against human immunodeficiency virus (hiv-1 or hiv-2) and various other rna and dna viruses. with regard to their anti-hiv activity, a mi ... | 1997 | 9022800 |
| a novel western blot test containing both viral and recombinant proteins for anticytomegalovirus immunoglobulin m detection. | we devised a novel western blot (wb) test for anti-human cytomegalovirus (hcmv) immunoglobulin m (igm) detection which contains viral structural polypeptides, significant portions of recombinant p150 (ppul32), and a significant portion of the most immunogenic nonstructural protein p52 (ppul44). this new test was evaluated in latently infected blood donors, pregnant women, and transplant recipients with ongoing hcmv infection and shown to be more sensitive and specific than traditional wb and con ... | 1997 | 9003603 |
| evaluation of a novel lipid prodrug for intraocular drug delivery: effect of acyclovir diphosphate dimyristoylglycerol in a rabbit model with herpes simplex virus-1 retinitis. | acyclovir diphosphate dimyristoylglycerol is a lipid prodrug of acyclovir that forms liposomes and provides substantial activity against herpes simplex virus, acyclovir-resistant strains of herpes simplex virus, and human cytomegalovirus. we therefore tested this promising new drug in a rabbit model of herpes simplex retinitis. | 1997 | 9051844 |
| design, synthesis, and antiviral evaluation of 2-chloro-5,6-dihalo-1-beta-d-ribofuranosylbenzimidazoles as potential agents for human cytomegalovirus infections. | 2-chloro-5,6-difluorobenzimidazole (8) was prepared from 4,5-difluoro-2-nitroaniline (5) via successive reduction, cyclization, and diazotization reactions. 2-chloro-5,6-dibromobenzimidazole (10) was obtained by a direct bromination of 2-chlorobenzimidazole (9) with bromine-water. 2-chloro-5,6-diiodobenzimidazole (15) was synthesized by a stepwise transformation of the nitro functions of 2-chloro-5,6-dinitrobenzimidazole (11) into iodo groups via diazotization reactions. ribosylation of 8, 10, a ... | 1997 | 9057868 |
| human cytomegalovirus us2 destabilizes major histocompatibility complex class i heavy chains. | human cytomegalovirus (hcmv) infection causes down-regulation of major histocompatibility complex class i heavy chains. we determined previously that there are two hcmv loci which encode functions responsible for that phenotype and that us11 is one of these loci (t. r. jones, l. a. hanson, l. sun, j. s. slater, r. m. stenberg, and a. e. campbell, j. virol. 69:4830-4841, 1995). through the construction and analysis of defined viral mutants and stably transfected cell lines, we identify us2 as the ... | 1997 | 9060656 |
| the immediate early gene of human cytomegalovirus stimulates vascular smooth muscle cell proliferation in vitro and in vivo. | cytomegalovirus (cmv) infection has been carefully studied regarding the relationship with the vascular smooth muscle cell (vsmc) proliferation in either atherosclerosis or post-angioplasty restenosis, but its role in the vessel wall has yet to be elucidated. to clarify the pathogenic ability of cmv in the vessel wall, we transduced the immediate early (ie) gene of cmv into the vsmcs and endothelial cells (ecs) by hemagglutinating virus of japan-liposome method. the in vitro ie gene transfer dem ... | 1997 | 9070298 |
| ganciclovir-loaded polymer microspheres in rabbit eyes inoculated with human cytomegalovirus. | to test the antiviral effect of ganciclovir released from biodegradable polymer microspheres in rabbit eyes inoculated with human cytomegalovirus (hcmv). | 1997 | 9071221 |
| interleukin-10 immunoadhesin production by a replication-defective adenovirus. | the present study examined the use of replication-defective adenovirus for in vitro production of an immunoadhesin. a recombinant adenovirus, rendered replication defective by deletion of the e1 gene, was constructed to contain the murine interleukin-10 gene fused in frame with the hinge, ch2, and ch3 domains of the murine immunoglobulin gamma 1 heavy chain constant region gene under the control of the human cytomegalovirus promoter. the resultant recombinant virus, ad5.hcmv.mil-10:hfc, was used ... | 1997 | 9075770 |
| successful quantification of cytomegalovirus dna by competitive pcr and detection with capillary electrophoresis. | human cytomegalovirus (hcmv) is responsible for severe infections in immunocompromised patients. viral load has recently been identified as one of the major risk factors for subsequent development of hcmv disease. in this context, we developed a protocol allowing rapid, sensitive and precise quantification of hcmv dna using competitive pcr run to saturation. long primers were used for amplification, and internal dna standard was constructed by pcr, with a primer inducing formation of a loop on t ... | 1997 | 9076710 |
| the class i mhc homologue of human cytomegalovirus inhibits attack by natural killer cells. | recognition and destruction of virus-infected cells by class i major histocompatibility complex (mhc) restricted cytotoxic t lymphocytes (ctl) is a central part of the immune system's attempts to control and eliminate virus infection. it is therefore not surprising that many viruses have evolved strategies to interfere with the processing and presentation of peptide antigen on class i mhc molecules (reviewed in ref. 1). these mechanisms act to prevent or reduce expression of mhc molecules at the ... | 1997 | 9087413 |
| alterations in the expression of elam-1, icam-1 and vcam-1 after in vitro infection of endothelial cells with a clinical isolate of human cytomegalovirus. | human cytomegalovirus (hcmv) infection of endothelial cells resulted in increased adhesion of the cells to peripheral blood leukocytes. it was demonstrated by flow cytometry that increased adhesiveness parallels the increased expression of cell surface adhesion molecules (elam-1, icam-1, vcam-1). the increased adhesion of pmn and t-lymphocytes was due to upregulation in the expression of elam-1 and icam-1. the upregulation of vcam-1 resulted in the increased adhesiveness of monocytes and t-lymph ... | 1997 | 9087954 |
| a model system for human cytomegalovirus-mediated modulation of human immunodeficiency virus type 1 long terminal repeat activity in brain cells. | previously, our laboratory showed that human cytomegalovirus (hcmv) activates human immunodeficiency virus type 1 (hiv-1) in brain-derived cells with limited hiv-1 gene expression but inhibits hiv-1 in cells fully permissive for replication of both viruses (f. m. jault, s. a. spector, and d. h. spector, j. virol. 68:959-973, 1994). to investigate these effects further, we developed a model system that uncouples hiv-1 gene expression from long terminal repeat (ltr) activity. two monoclonal u373-m ... | 1997 | 9094643 |
| human cytomagalovirus ie1 and ie2 proteins are mutagenic and mediate "hit-and-run" oncogenic transformation in cooperation with the adenovirus e1a proteins. | some epidemiological studies have suggested a possible link between human cytomegalovirus (hcmv) infection and various malignancies, and hcmv has been shown to transform cultured cells. however, viral dna is not detected in most transformants, and the mechanism by which hcmv might contribute to oncogenesis has remained obscure. here we show that the hcmv immediate early 1 and 2 genes can cooperate with the adenovirus e1a gene to generate transformed foci of primary baby rat kidney cells. hcmv ge ... | 1997 | 9096395 |
| quantitative systemic and local evaluation of the antiviral effect of ganciclovir and foscarnet induction treatment on human cytomegalovirus gastrointestinal disease of patients with aids. italian foscarnet gid study group. | in a group of 29 aids patients with biopsy-proven human cytomegalovirus (hcmv) gastrointestinal disease (gid), hcmv gid was shown to correlate mostly with systemic hcmv infection. the antiviral induction treatment (it) with either ganciclovir (gcv) or foscarnet (pfa) caused a significant reduction in the level of hcmv antigenemia, viremia and leukodnaemia, and a complete virus clearance or a sharp drop of viral load in the blood of 13/13 patients and in the gastrointestinal (gi) mucosa of 12/13 ... | 1997 | 9107384 |
| genetic analysis of a clinical isolate of human cytomegalovirus exhibiting resistance against both ganciclovir and cidofovir. | both ganciclovir-sensitive and -resistant human cytomegaloviruses (hcmv) were isolated from a patient with aplastic anemia complicated with cmv retinitis and encephalitis. ganciclovir-resistant clinical isolate, 93-1r, also showed cross-resistance against (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl) cytosine (cidofovir). molecular analysis of plaque-cloned strains revealed that a single nucleotide substitution at 2160 (c to t) resulted in amino acid substitution at codon 501 from leucine to pheny ... | 1997 | 9125039 |
| measurement of specific protease activity utilizing fluorescence polarization. | a fluorescence polarization assay was designed to measure proteolytic cleavage of a specific peptide substrate for human cytomegalovirus protease. the peptide substrate was derivatized by biotinylation of a gamma-aminobutyric acid-modified amino-terminus and labeled with 5-(4,6-dichlorotriazinyl)aminofluorescein at the carboxy-terminus. incubation of this substrate with recombinant human cytomegalovirus protease and subsequent addition of egg white avidin produced a polarization signal that was ... | 1997 | 9126375 |
| comparison of the human versus murine cytomegalovirus immediate early gene promoters for transgene expression by adenoviral vectors. | we have developed a number of replication defective adenoviral (ad) vectors which express transgenes under the control of the human cytomegalovirus (hcmv) immediate early (ie) gene promoter. the orientation of the expression cassette replacing e1 in the vector backbone had a significant effect on the level of transgene expression, with vectors containing expression cassettes directed towards the right end of the ad genome expressing 7-fold higher levels of beta-galactosidase (beta-gal) than thos ... | 1997 | 9225042 |
| use of recombinant human antibody fragments for detection of cytomegalovirus antigenemia. | the determination and quantitation of peripheral blood leukocytes (pbls) expressing human cytomegalovirus (hcmv) antigens is widely employed in clinical virology for rapid diagnosis of hcmv-related infections. we describe how cmv antigenemia may be accurately detected by means of human recombinant monoclonal fab fragments rescued from a combinatorial phage display library prepared from an hcmv-infected donor. fourteen recombinant fabs were tested against hcmv-positive pbls from a patient with on ... | 1997 | 9230379 |
| clinical significance of qualitative human cytomegalovirus (hcmv) detection in cell-free serum samples in hiv-infected patients at risk for hcmv disease. | 1997 | 9233475 | |
| treatment of ganciclovir-resistant human cytomegalovirus infection. | human cytomegalovirus (hcmv) is the most frequent cause of infectious complications after whole organ transplantation. ganciclovir is a nucleoside analogue of guanosine, and the first antiviral drug to be effective in the treatment of cytomegalovirus disease in humans. however, prolonged or repeated courses of ganciclovir predispose to the development of viral drug resistance. the isolation of a ganciclovir-resistant virus strain was first reported in 1989. mutations in ul97 and dna polymerase g ... | 1997 | 9238622 |
| focus luminescence assay: macroscopically visualized foci of human cytomegalovirus and varicella zoster virus infection. | the plaques or foci of certain viruses due to their small size have to be counted microscopically, e.g., human cytomegalovirus (hcmv). the focus luminescence assay (fla) described below generates macroscopic images as a result of the magnification due to scattered emitted light, and provides a hard copy using autoradiography or video imaging. foci are detected according to an immunohistochemical protocol with horseradish peroxidase or alkaline phosphatase antibody conjugates which convert substr ... | 1997 | 9255742 |
| preclinical testing of recombinant adenoviral herpes simplex virus-thymidine kinase gene therapy for central nervous system malignancies. | adenoviral gene transfer and killing efficiency using the thymidine kinase (tk)/ganciclovir (gcv) mechanism was evaluated in human cancer cells occurring as central nervous system tumors. the effectiveness of this approach was tested in vitro and in experimental models for brain tumor and leptomeningeal metastases in rats in vivo. recombinant adenoviruses with different promoters were compared. | 1997 | 9257313 |
| modulation of rantes production by human cytomegalovirus infection of fibroblasts. | chemokines play a major role in inflammatory responses and affect hematopoiesis both negatively and positively. we show that fresh isolates and laboratory strains (towne and ad-169) of human cytomegalovirus (hcmv) induce production of the cc chemokine rantes in fibroblasts. induction of extracellular rantes production occurred as early as 8 h after infection, peaked around 24 h after infection, and was almost undetectable by 48 and 72 h. upregulation occurred in the absence of viral dna synthesi ... | 1997 | 9261368 |
| binding of sp1 to the immediate-early protein-responsive element of the human cytomegalovirus dna polymerase promoter. | human cytomegalovirus (hcmv), a member of the herpesvirus family of dna viruses, encodes two major immediate-early (ie) transcription factors, ie72 and ie86, that are important for regulated expression of the viral genome. the purpose of this study was to identify the host cellular components required for regulation of the hcmv dna polymerase promoter (ul54) by hcmv ie proteins. extensive mutagenesis defined a dna element located between -54 and -43 relative to the transcription start site that ... | 1997 | 9261391 |
| an in vivo model of human cytomegalovirus retinal infection. | to develop an animal model system in which human retina implanted in the anterior chamber of the eyes of rats would support human cytomegalovirus replication. cytomegalovirus retinitis currently represents the most common cause of posterior uveitis in many urban areas in north america. despite the tremendous interest in cytomegalovirus retinitis as a result of the acquired immunodeficiency syndrome (aids) epidemic, human cytomegalovirus infection has been difficult to model in vivo because of it ... | 1997 | 9262541 |
| identification of the gene coding for rhesus cytomegalovirus glycoprotein b and immunological analysis of the protein. | the nucleotide sequence of the gene encoding glycoprotein b (gb) of rhesus cytomegalovirus (rhcmv) was determined and the protein characterized. the open reading frame of gb encoded a protein of 854 amino acids with 60% identity and 75% similarity at the amino acid level to human cytomegalovirus (hcmv) gb. cysteine residues in the extraluminal part of the protein are perfectly conserved. out of the 16 potential n-linked glycosylation sites present in hcmv gb, 15 are conserved in rhcmv gb. immuno ... | 1997 | 9267000 |
| identification of envelope glycoproteins h and b homologues of human herpesvirus 7. | the genes encoding the envelope glycoprotein h (gh) and gb homologues were identified by sequencing genomic clones of human herpesvirus-7 (hhv-7), strain ji. a gb cdna clone from hhv-7 strain al was also identified. the deduced primary translation products of the gh and gb genes are a protein of 690 amino acids, with a predicted mass of 80.4 kd, and a protein of 822 amino acids, with a predicted mass of 93.3 kd, respectively. both the predicted proteins have the characteristics of transmembrane ... | 1997 | 9268767 |
| pharmacokinetics of a potential human cytomegalovirus therapeutic, a phosphorothioate oligonucleotide, after intravitreal injection in the rabbit. | the disposition of isis 2922, a phosphorothioate oligonucleotide for treatment of cytomegalovirus associated retinitis, was evaluated in rabbits. vitreous humor and retina samples were collected from rabbits that received a single intravitreal injection of 66 microg [14c]-labeled isis 2922 and were analyzed using anion exchange hplc. four hr postdosing, the concentration of isis 2922 in vitreous humor was 3.3 microm. the elimination of isis 2922 from the vitreous humor exhibited first-order kine ... | 1997 | 9280399 |
| a macrophage-like cell model for testing anti-cmv drugs. | monocyte/macrophage cells can be infected with human cytomegalovirus (hcmv) and could be a reservoir and a vehicle for virus spread in infected patients. the monocytic-like cell line thp-1 differentiated along the monocyte pathway with a phorbol ester (phorbol 12-myristate 13-acetate, pma) for 24h was infected with cell free-supernatant of towne cmv-strain infected fibroblasts mrc-5. we designed experiments to study the replication of hcmv in pma treated thp-1 cells, exposed to dhpg (cymevan, ga ... | 1997 | 9296091 |
| value of a ligase chain reaction assay for detection of ganciclovir resistance-related mutation 594 in ul97 gene of human cytomegalovirus. | human cytomegalovirus (hcmv) isolates resistant to ganciclovir were found in patients undergoing therapy. therefore, we have developed a new specific and sensitive method--a ligase chain reaction (lcr) assay--for detection of frequently encountered 594 mutated codon in ganciclovir (gcv) resistant virus. previous studies characterized an alanine to valine change on codon 594 in resistant strains. a novel substitution in 594, alanine to glycine, is described which is also capable of conferring gan ... | 1997 | 9300382 |
| taf-like functions of human cytomegalovirus immediate-early proteins. | the human cytomegalovirus (hcmv) major immediate-early (ie) proteins iep86 (ie2(579aa)) and iep72 (ie1(491aa)) can transcriptionally activate a variety of simple promoters containing a tata element and one upstream transcription factor binding site. in our previous studies, transcriptional activation was shown to correlate with iep86 binding to both the tata-box binding protein (tbp) and the transcription factor bound upstream. iep72 often synergistically affects the activation by iep86, althoug ... | 1997 | 9311796 |
| differential effects of cytomegalovirus infection on complement synthesis by human mesangial cells. | viruses may be eliminated by the host immune system via complement-mediated lysis of infected cells. previously, we have demonstrated that the synthesis of complement factor b by renal mesangial cells (mc) is enhanced by interferon-alpha (ifn-alpha), -beta and -gamma. in the present study we investigate the effect of human cytomegalovirus (hcmv) infection on the production of complement factors by mc. the production of factor b, c2, c4 and factor h by mock-infected mc was 0.2 +/- 0.4, 3.9 +/- 6. ... | 1997 | 9328131 |
| hierarchical affinity maturation of a phage library derived antibody for the selective removal of cytomegalovirus from plasma. | recombinant antibody fragments can be produced in large quantities using bacterial expression systems and could potentially be useful for the generation of biofilters for the selective removal of viral particles from fluids. a human single chain-fv antibody library, derived from synthetic repertoires of germ line vh-gene segments rearranged in vitro and paired to a single light chain (nissim et al., 1994, embo j., 13, 692-698), has recently been used to isolate hundreds of different binding spec ... | 1997 | 9328580 |
| formation of cytomegalovirus dna replication compartments defined by localization of viral proteins and dna synthesis. | to characterize the formation of replication compartments in human cytomegalovirus-infected cells, and to determine the fate of newly synthesized dna, we localized viral replication proteins and dna synthesis at early and late times during infection. as expected, ppul57 (single-stranded dna binding protein) and ppul44 (dna polymerase processivity factor) both localized to replication compartments beginning at 48 hpi. brdu was incorporated into viral dna in these compartments that was found to ma ... | 1997 | 9426445 |
| reassessing the organization of the ul42-ul43 region of the human cytomegalovirus strain ad169 genome. | a polymorphism in the ul42-ul43 region of the human cytomegalovirus genome has been characterized by nucleotide sequence analysis, revealing a 929-bp insertion following nt 54,612 relative to the published strain ad169-uk genome sequence (m.s. chee et al., 1990, curr. top. microbiol immunol. 154, 125-170). although ad169-uk exhibited polymorphism in this genomic region, other cmv strains (towne, toledo, and ad169-atcc) carried only the newly characterized longer form. the additional sequence alt ... | 1997 | 9426456 |
| expression, purification, and characterization of a soluble form of human cytomegalovirus glycoprotein b. | the human cytomegalovirus glycoprotein b gene (gb; gpul55) was truncated at amino acid 692 and recombined into autographa californica nuclear polyhedrosis virus (baculovirus). infection of insect cells with the recombinant baculovirus resulted in high-level expression and secretion of the truncated gb protein (gb-s) into the culture medium. purification of gb-s by monoclonal antibody affinity chromatography yielded a protein of ca. 200 kda. characterization of the 200-kda purification product in ... | 1997 | 9426459 |
| gene expression by atypical recombinant ovine adenovirus vectors during abortive infection of human and animal cells in vitro. | an bovine adenovirus, which is phylogenetically distinct from the mastadeno- and aviadenoviruses, was used to construct recombinants in which reporter genes were expressed from the oav major late, or human cytomegalovirus promoters. it was demonstrated by transgene expression that oav could infect bovine nasal turbinate and rabbit kidney cells as well as a range of human cell types, including lung and foreskin fibroblasts as well as liver, prostate, breast, colon, and retinal lines. some human l ... | 1997 | 9426462 |
| synthesis and antiviral activity of 1-o-octadecyl-2-o-alkyl-sn-glycero-3-foscarnet conjugates in human cytomegalovirus-infected cells. | a series of new lipid prodrugs with the general structure, 1-o-octadecyl-2-x-sn-glycero-3-pfa were synthesized and evaluated for antiviral activity in hcmv-infected human lung fibroblasts (x is -h, -oh or an o-alkyl group of increasing chain length) in order to study structure-activity relationships of pfa lipid prodrugs. the ec50 values for the 2-o-octyl, 2-o-butyl, 2-h, 2-oh, 2-o-methyl and 2-o-ethyl substituted analogs were 1.96, 0.36, 1.0, 0.7, 0.53 and 0.18 microm respectively versus 40 mic ... | 1997 | 9443668 |
| the human cytomegalovirus ul98 gene encodes the conserved herpesvirus alkaline nuclease. | the human cytomegalovirus (hcmv) ul98 gene is predicted to encode a homologue of the conserved herpesvirus alkaline nuclease. to determine if the hcmv ul98 gene product is functionally homologous to other herpesvirus alkaline nucleases, the hcmv ul98 protein was purified and its activity characterized in vitro. extracts of hcmv-infected cells were fractionated using q sepharose, phosphocellulose and native dna cellulose chromatography. ul98 immunoreactivity copurified with alkaline ph-dependent ... | 1997 | 9367383 |
| expression of the catalytic subunit (ul54) and the accessory protein (ul44) of human cytomegalovirus dna polymerase in a coupled in vitro transcription/translation system. | the catalytic subunit (ul54) and accessory protein (ul44) of human cytomegalovirus (hcmv) dna polymerase have been cloned and expressed in an in vitro-coupled transcription/translation reticulocyte lysate system. the influence of the 5'-untranslated region (5'-utr) on the efficiency of expression from the circular plasmids has been investigated. for expression of both ul54 and ul44, a truncated form of the alfalfa mosaic virus (amv) rna4 5'-utr was found to be superior to the full-length amv 5'- ... | 1997 | 9367818 |
| interrelationships among quantity of human cytomegalovirus (hcmv) dna in blood, donor-recipient serostatus, and administration of methylprednisolone as risk factors for hcmv disease following liver transplantation. | longitudinal analysis of 162 liver transplant recipients identified 51 patients who were viremic. virus load was determined in 47 of these patients using quantitative-competitive polymerase chain reaction. peak virus load was significantly higher in 20 symptomatic patients than 27 asymptomatic patients (p < .0001). elevated virus load, donor seropositivity, and total methylprednisolone dosage were risk factors for human cytomegalovirus (hcmv) disease (odds ratio [or], 2.22/0.25 log10 increase in ... | 1997 | 9395358 |
| efficacy of replication-defective adenovirus-vectored vaccines: protection following intramuscular injection is linked to promoter efficiency in muscle representative cells. | to investigate the respective role of transduced cells in the induction of immune response following intramuscular inoculation of adenovirus-based vaccines, we generated several replication-defective adenoviruses expressing the glycoprotein d gene of pseudorabies virus under the control of four different promoters: major late promoter of adenovirus type 2, human cytomegalovirus immediate-early promoter/enhancer (cmv), rous sarcoma virus-long terminal repeat promoter, and human desmin gene 5' reg ... | 1997 | 9400605 |
| immune response to neutralizing epitope on human cytomegalovirus gylcoprotein b in japanese: correlation of serologic response with hla-type. | antigenic domain 1 (ad-1), located between amino acids 608 and 625 of human cytomegalovirus (cmv) gb protein, is the major domain recognized by neutralizing antibodies. amino acids 552 to 630 are essential for the binding of neutralizing antibodies. we developed an enzyme-linked immunosorbent assay (elisa) to detect antibodies against a fusion protein containing amino acid residues 549 to 644 of the gb polypeptide and maltose binding protein (mbp). of 180 seropositive samples, 106 (58.9%) showed ... | 1997 | 9403513 |
| [relationship between human cytomegalovirus infection and soluble interleukin-2 receptor level]. | in order to study the relationship between human cytomegalovirus infection and soluble interleukin-2 receptor (sil-2r) level, 104 serum samples were selected from women of childbearing age in the city of wuhan and examined with elisa for anti-hcmv antibody igg and igm. then we detected the sil-2r level with double antibody elisa in these sera with different conditions of hcmv infection. the results of different groups were compared with those women without cytomegalovirus infection and also comp ... | 1997 | 15617342 |
| [the carcinogenesis of human cytomegalovirus to mouse cervix]. | we plugged the gelfoam impregnated with virus stock solution into mouse cervix with ophthalmic forceps. mice of kun-ming strain were infected with uv-inactivated hcmv (human cytomegalovirus). after 31 weeks, cervical carcinomas were induced in 15% of the infected mice. in another group, infected with uv-inactivated hcmv adding croton oil, the carcinogenesis was 18.8%, but incontrol group, it was 0%. hcmv ie (immediate early) antigen in 12 cancer tissues of uv-inactivated hcmv treated group were ... | 1997 | 15619897 |
| molecular basis for the antiviral and anticancer activities of unnatural l-beta-nucleosides. | as a general rule, enzymes act on only one enantiomer of a chiral substrate and only one of the enantiomeric forms of a chiral molecule may bind effectively at the catalytic site, displaying biological activity. in recent years, some exceptions have been found among viral and cellular enzymes involved in the synthesis of deoxynucleoside triphosphates and in their polymerisation into dna. examples are: herpes virus thymidine kinases, cellular deoxycytidine kinase and deoxynucleotide kinases, huma ... | 1998 | 15992031 |
| new adenovirus vectors for protein production and gene transfer. | based on two new adenovirus expression cassettes, we have constructed a series of ad transfer vectors for the overexpression of one or two genes either in a dicistronic configuration or with separate expression cassettes. inclusion of the green or blue fluorescent protein in the vectors accelerates the generation of adenovirus recombinants and facilitates the functional characterization of genes both in vitro and in vivo by allowing easy quantification of gene transfer and expression. with our o ... | 1998 | 19003407 |
| characterization of a murine cytomegalovirus class i major histocompatibility complex (mhc) homolog: comparison to mhc molecules and to the human cytomegalovirus mhc homolog. | both human and murine cytomegaloviruses (hcmv and mcmv) down-regulate expression of conventional class i major histocompatibility complex (mhc) molecules at the surfaces of infected cells. this allows the infected cells to evade recognition by cytotoxic t cells but leaves them susceptible to natural killer cells, which lyse cells that lack class i molecules. both hcmv and mcmv encode class i mhc heavy-chain homologs that may function in immune response evasion. we previously showed that a solubl ... | 1998 | 9420246 |
| prokaryotic expression of human cytomegalovirus pus22 and its reactivity with human antibody. | this work demonstrates that antibodies to the product of the recombinant pus22 of human cytomegalovirus (hcmv) are present in human sera during natural infection. us22 gene product has been identified as a member of the us22 family which may be secreted from infected cells. it is an early protein of 593 amino acids, 76 kd in molecular weight. us22 seems to be an antigen which stimulates a good igg response. in fact specific iggs were found in approximately 40% of the cmv positive sera irrespecti ... | 1998 | 9930196 |
| structure of the human cytomegalovirus b capsid by electron cryomicroscopy and image reconstruction. | the three-dimensional structure of b capsids of the beta-herpesvirus human cytomegalovirus (hcmv) was investigated at a resolution of 3.5 nm from electron cryomicrographs by image processing and compared with the structure obtained for the alpha-herpesvirus herpes simplex virus type 1 (hsv-1). the main architectural features of the hsv-1 and hcmv capsids are similar: the t = 16 icosahedral lattice consists of 162 capsomers, composed of two distinct morphological units, 12 pentamers and 150 hexam ... | 1998 | 9931275 |
| human herpesvirus-6 (hhv-6) infection in multiple sclerosis: a preliminary report. | we examined cerebral spinal fluid (csf) from multiple sclerosis (ms) patients and patients with other neurological diseases (ond) for antibody specific for human herpesvirus-6 (hhv-6) and for hhv-6 dna detectable by pcr. csf from ms patients had a higher frequency of igg antibody to hhv-6 late antigens (39.4%) compared with csf from ond (7.4%). in contrast, the frequency of detectable igg antibody in csf from ms patients specific for epstein-barr virus (ebv) (12.1%) and human cytomegalovirus (hc ... | 1998 | 9987758 |
| [human cytomegalovirus infection and congenital malformation]. | to study the relationship between intrauterine cytomegalovirus (hcmv) infection and congenital malformation, and to determine the distribution of tissues infected. | 1998 | 10682476 |
| [detection of human cytomegalovirus infection in pregnant women and their fetuses by nested polymerase chain reaction]. | to evaluate the applicability of nested polymerase chain reaction (pcr) and restriction endonucleases analyses (rea) for the diagnosis of human cytomegalovirus (hcmv) in pregnant women and their fetuses. | 1998 | 10682477 |
| human papillomavirus and human cytomegalovirus dnas presence in patients suspected of condylomatosis or papillomatosis. | 17 samples of total cell dna isolated from cervical smears from women suspected of condyloma or papilloma were analysed by pcr with appropriate primers, in order to establish the presence of viral dnas (hpv and/or hcmv). hpv dna was found in seven cases, and so was for hcmv dna. only in three cases a coinfection was present. the rflp allowed to specify the involvement of hpv6 in 3 cases suspected of condyloma and in one suspected of papilloma; the other three hpv positive samples had another gen ... | 1998 | 10892429 |
| foamy virus particle formation. | subgenomic expression plasmids for the so-called human foamy virus (hfv) structural gag, gag/pol, and env genes were constructed and used to analyze foamy virus particle formation by electron microscopy. expression of an r-u5-gag-pol construct under control of the human cytomegalovirus immediate-early enhancer-promoter resulted in the formation of viral cores with a homogeneous size of approximately 50 nm located in the cytoplasm. upon coexpression of an envelope construct, particles were observ ... | 1998 | 9445065 |
| sharp drop in the prevalence of human cytomegalovirus leuko-dnaemia in hiv-infected patients following highly active antiretroviral therapy. | 1998 | 9456267 | |
| [some important clinical aspects of cytomegalovirus infection]. | some important aspects of human cytomegalovirus (cmv) infection were discussed from a clinical point of view. congenital infection: the mass-screening of pregnant women is not justified at present, mainly because of limited value of diagnostic methods for detecting fetal infection and unavailability of effective measure for prevention. extensive diagnostic approach and intrauterine therapy may be applicable in the case of intrauterine growth retardation or abnormal ultrasonographic findings. dia ... | 1998 | 9465658 |
| [the function of human cytomegalovirus genes]. | the genome of human cytomegalovirus(hcmv) has been completely sequenced and encodes more than 200 genes. approximately 40 genes have been identified as "core genes" which are conserved in other members of the herpesvirus family. these include a set of genes required for viral dna replication and virion morphogenesis. the hcmv genome also encodes homologs of cellular gene products such as mhc class i heavy chain, t cell gamma chain receptor and c-c chemokine receptor. several genes in the us regi ... | 1998 | 9465661 |
| [the receptor and pathways for human cytomegalovirus entry]. | the cellular receptor for human cytomegalovirus(hcmv) entry has not been identified. to date, heparan sulfate proteoglycan, annexin ii, and cd13(aminopeptidase n) are reported to be associated with hcmv entry into human fibroblast cells. a recombinant hcmv that can express a marker protein on the infected cell was generated and used to assess relationship between hcmv entry and expression of heparan sulfate and cd13. the recombinant virus could infect to two human neuroblastoma-derived cell line ... | 1998 | 9465663 |
| [pathology of human cytomegalovirus infection in immunocompromised hosts]. | we reviewed the histopathological diagnosis of human cytomegalovirus (hcmv) and the pathology of hcmv infection in immunocompromised hosts. for histological diagnosis, routine staining of paraffin sections of formalin fixed tissue obtained at biopsy and autopsy is important to detect cytomegalic inclusion (owl's eye). for confirmation of hcmv diagnosis, immunohistological detection of immediate early and structural proteins of hcmv is valuable. cytomegalic inclusion contains a cowdry a inclusion ... | 1998 | 9465675 |
| [utility of quantitation of human cytomegalovirus(hcmv) dna in plasma and cerebrospinal fluid by quantitative competitive pcr in aids patients]. | persons with advanced hiv infection may have active hcmv infection for months before clinically recognized hcmv disease. the qualitative detection of hcmv-dna in plasma by pcr is more useful than either urine or blood culture in identifying over time those persons with advanced hiv disease who will develop hcmv disease. quantitation of hcmv dna in plasma has identified an association between levels of hcmv dna in plasma and risk for development of hcmv disease. thus, quantitative competitive pcr ... | 1998 | 9465678 |
| [analysis of ganciclovir-resistant cytomegalovirus]. | the ganciclovir(gcv)-resistant human cytomegaloviruses(hcmv) result from the mutation(s) in either the ul97 phosphotransferase gene, or ul54 dna polymerase gene, or both. majority of clinical isolates of gcv-resistant mutant had mutations in the ul97 gene. because the mutations in the ul97 gene cluster in two regions, sudomain vi and ix, it is almost possible to identify a gcv-resistant mutant by pcr-based dna analysis. on the contrary, only a few mutants which had mutation(s) in the ul54 dna po ... | 1998 | 9465681 |
| human cytomegalovirus inhibits major histocompatibility complex class ii expression by disruption of the jak/stat pathway. | human cytomegalovirus (hcmv) is a ubiquitous herpesvirus that is able to persist for decades in its host. hcmv has evolved protean countermeasures for anti-hcmv cellular immunity that facilitate establishment of persistence. recently it has been shown that hcmv inhibits interferon gamma (ifn-gamma)-stimulated mhc class ii expression, but the mechanism for this effect is unknown. ifn-gamma signal transduction (jak/stat pathway) and class ii transactivator (ciita) are required components for ifn-g ... | 1998 | 9480977 |
| false positive igm antibody tests for human cytomegalovirus (hcmv) in patients with sle. | 1998 | 9493154 | |
| interpreting human cytomegalovirus antiviral drug susceptibility testing: the role of mixed virus populations. | 1998 | 9498475 | |
| distinct pathways for tumor necrosis factor alpha and ceramides in human cytomegalovirus infection. | human cytomegalovirus (hcmv) infection can be fatal to immunocompromised individuals. we have previously reported that gamma interferon and tumor necrosis factor alpha (tnf-alpha) synergistically inhibit hcmv replication in vitro. ceramides have been described as second messengers induced by tnf-alpha. to investigate the mechanisms involved in the inhibition of hcmv by tnf-alpha, in the present study we have analyzed ceramide production by u373 mg astrocytoma cells and the effects of tnf-alpha v ... | 1998 | 9499092 |
| evaluation and mapping of the dna binding and oligomerization domains of the ie2 regulatory protein of human cytomegalovirus using yeast one and two hybrid interaction assays. | the 86-kda ie2 nuclear phosphoprotein encoded by the human cytomegalovirus (hcmv) major immediate-early (mie) gene behaves as both a non-specific transactivator of viral and cellular gene expression and as a specific dna-binding protein targeted to the cis-repression sequence (crs) at the cap site of its own promoter/enhancer region. although the ie2 protein produced in bacteria has been shown to bind to the 14-bp palindromic crs motif and ie2 synthesized in vitro forms stable dimers in solution ... | 1998 | 9524210 |
| rising levels of human cytomegalovirus (hcmv) antigenemia during initial antiviral treatment of solid-organ transplant recipients with primary hcmv infection. | in 7 of 18 solid-organ transplant recipients with primary human cytomegalovirus (hcmv) infection, hcmv antigenemia levels were unexpectedly found to rise significantly (p = 0.018) during a mean time of 7.3 +/- 3.2 days after initiation of specific antiviral treatment, whereas corresponding levels of viremia dropped significantly (p = 0.043). thus, shifting to an alternative antiviral drug based solely on increasing antigenemia levels is not justified in this group of patients. | 1998 | 9542949 |
| design, synthesis, and antiviral evaluations of 1-(substituted benzyl)-2-substituted-5,6-dichlorobenzimidazoles as nonnucleoside analogues of 2,5,6-trichloro-1-(beta-d-ribofuranosyl)benzimidazole. | we have recently reported that certain ribosylated polyhalogenated benzimidazoles are potent and selective inhibitors of hcmv replication at noncytotoxic concentrations. to extend the structure-activity relationship beyond these first-generation compounds, we alkylated 5,6-dichloro-2-substituted-benzimidazoles with either a series of substituted benzyl halides or (2-bromoethyl)benzene to obtain five series of nonnucleoside analogues. evaluation of these compounds for activity against herpes viru ... | 1998 | 9548815 |
| site-directed mutagenesis probing the catalytic role of arginines 165 and 166 of human cytomegalovirus protease. | human cytomegalovirus (cmv) is a member of the herpesviridae family of viruses that also includes herpes simplex viruses (hsv-1 and hsv-2), varicella-zoster virus (vzv), human herpes virus-6, 7, and 8 (hhv-6, hhv-7, and hhv-8), and epstein-barr virus (ebv). each member of this family encodes a serine protease that is a potential target for antiviral therapeutic intervention. we recently reported the crystal structure of cmv proteases [qiu, x., culp, j. s., dilella, a. g., hellmig, b., hoog, s. s ... | 1998 | 9558326 |
| a dna vaccine protects mice against the rickettsial agent cowdria ruminantium. | a dna vaccine (vcl1010/map1) containing the major antigenic protein 1 (map1) gene of cowdria ruminantium, driven by the human cytomegalovirus (hcmv) enhancer-promoter, was injected intramuscularly into 8-10 week-old female dba/2 mice after treating them with 50 microliters/muscle of 0.5% bupivacaine three days previously. up to 75% of the immunized mice seroconverted and reacted with c. ruminantium antigen blots. splenocytes from immunized mice, but not from control mice, proliferated in respons ... | 1998 | 9568614 |
| intradermal immunization with a bovine herpesvirus-1 dna vaccine induces protective immunity in cattle. | although intramuscular (i.m.) injection of dna encoding glycoprotein d (gd) of bovine herpesvirus-1 (bhv-1) induces immune responses in cattle, this route of delivery is inefficient. here we assessed three parameters that may enhance the efficacy of a gd dna vaccine in cattle. first, the immune response generated by i.m. injected plasmid expressing a secreted form of gd (tgd) was determined and found to be very similar in magnitude to the response induced by gd-expressing plasmid. secondly, gd- ... | 1998 | 9568979 |
| human cytomegalovirus glycoprotein h/glycoprotein l complex modulates fusion-from-without. | glycoprotein h/glycoprotein l (gh/gl) complexes of herpesviruses are required for fusion of infecting virions with host cell membranes. in human cytomegalovirus (hcmv), neutralizing monoclonal antibodies (mab) specific for gh inhibit the transfer of a fluorescent probe to the host cell from labelled virus particles. in similar fashion, in the present study, neutralizing gh-specific mab inhibited hcmv-induced fusion-from-without in monolayers of both human embryonic fibroblasts and continuous ast ... | 1998 | 9568982 |
| ribosomal release without peptidyl trna hydrolysis at translation termination in a eukaryotic system. | a 22-codon upstream open reading frame (uorf2) in the human cytomegalovirus ul4 transcript leader inhibits downstream translation in cis. previous studies revealed that the peptide product of uorf2 mediates this inhibitory effect by interfering with translation termination at its own stop codon. the block at termination results both in accumulation of the nascent uorf2 peptide linked to trna(pro), the trna that decodes the final codon of uorf2, and in stalling of ribosomes at the end of uorf2. t ... | 1998 | 9570317 |
| resistance of human cytomegalovirus to benzimidazole ribonucleosides maps to two open reading frames: ul89 and ul56. | 2,5,6-trichloro-1-beta-d-ribofuranosyl benzimidazole (tcrb) is a potent and selective inhibitor of human cytomegalovirus (hcmv) replication. tcrb acts via a novel mechanism involving inhibition of viral dna processing and packaging. resistance to the 2-bromo analog (bdcrb) has been mapped to the ul89 open reading frame (orf), and this gene product was proposed as the viral target of the benzimidazole nucleosides. in this study, we report the independent isolation of virus that is 20- to 30-fold ... | 1998 | 9573236 |
| human cytomegalovirus orilyt sequence requirements. | the mechanisms of action and regulation of the human cytomegalovirus (hcmv) lytic-phase dna replicator, orilyt, which spans more than 2 kbp in a structurally complex region near the middle of the unique long region (ul), are not understood. because orilyt is thought to be essential for promoting initiation of lytic dna synthesis and may participate in regulating the switch between lytic and latent phases, we undertook a mutational study to better define its sequence requirements. kanr gene casse ... | 1998 | 9573268 |
| circulating cytomegalic endothelial cells are associated with high human cytomegalovirus (hcmv) load in aids patients with late-stage disseminated hcmv disease. | the prevalence of circulating cytomegalic endothelial cells, detected currently by the pp65-antigenemia assay and described previously in blood of transplanted and aids patients with disseminated human cytomegalovirus (hcmv) infection, was found to be 2.9% in the aids population and 6.5% in the fraction of the aids population with hcmv in blood. cytomegalic endothelial cells increased to 39.7% and 48.4%, respectively, in aids patients with very high levels of antigenemia and viremia, while an en ... | 1998 | 9580888 |
| human cytomegalovirus in blood of immunocompetent persons during primary infection: prognostic implications for pregnancy. | human cytomegalovirus (hcmv) was investigated in peripheral blood leukocytes (pbl) of 52 immunocompetent patients (40 pregnant women) with primary hcmv infection by quantitative determination of pp65 antigenemia, viremia, and leukodnaemia. pp65 antigenemia was detected in 12 (57.1%) of 21, 4 (25%) of 16, and 0 of 10 patients examined 1, 2, and 3 months after onset, respectively. viremia was detected in 5 (26.3%) of 19 patients during the first month only. leukodnaemia was detected in 20 of 20, 1 ... | 1998 | 9592999 |
| the human cytomegalovirus ie2 86 kda protein elevates p53 levels and transactivates the p53 promoter in human fibroblasts. | human cytomegalovirus (hcmv), a herpesvirus, has previously been reported to elevate cellular p53 levels within infected human fibroblasts. since this elevation was reported to be due to the expression of hcmv immediate early (ie) gene products within the fibroblasts, and the hcmv immediate early region encodes several predominant proteins, the identity of the protein responsible for this elevation was investigated. we report that the hcmv ie2 86 kda protein was clearly found to be the major imm ... | 1998 | 9593583 |
| prenatal detection of human cytomegalovirus dna in fetal ascites by the polymerase chain reaction. | 1998 | 9598962 | |
| a fifth human cytomegalovirus glycoprotein b genotype. | genetic variation in glycoprotein b (gb) may play a role in human cytomegalovirus (hcmv) pathogenesis. using restriction endonuclease digestion and dna sequencing, a unique gb genotype was identified in eight hcmv strains isolated from five patients with the acquired immune deficiency syndrome. nucleic acid homology to the four previously described gb genotypes ranged from 79 to 91% for the two major variable regions of gb. studies of the role of gb in hcmv pathogenesis should recognize the exis ... | 1998 | 9602505 |
| clinical and microbiological evaluation of a bioabsorbable and a nonresorbable barrier membrane in the treatment of periodontal intraosseous lesions. | clinical and microbiological features of periodontal healing in barrier membrane-treated sites were determined in a randomized clinical trial. the study included 10 patients with advanced adult periodontitis and a minimum of one set of similar 2 to 3 wall intraosseous periodontal lesions with no furcation involvement. in each patient, one periodontal lesion was treated with a biodegradable membrane and a contralateral lesion with a nonresorbable barrier membrane. within the preceding 3 months of ... | 1998 | 9609375 |
| fasl induces fas/apo1-mediated apoptosis in human embryonic kidney 293 cells routinely used to generate e1-deleted adenoviral vectors. | human embryonic kidney 293 cells contain the e1 region of adenovirus type 5, and thus sustain, through transcomplementation, the production of recombinant e1-deleted adenovirus vectors. during attempts to produce recombinant adenovirus expressing the apoptosis-inducing molecule fas ligand (fasl) under the control of a very strong truncated major immediate-early human cytomegalovirus (miehcmv) promoter, we discovered that 293 cells were not surviving the initial cotransfection with a shuttle plas ... | 1998 | 9614583 |