Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
ribotyping and whole-cell protein analysis of spirochetes isolated from arthropods in the czech republic.in the presented work, six borrelia burgdorferi sensu lato isolates were recovered from ixodes ricinus ticks and one strain of spirochete with uncertain taxonomic position isolated from culex pipiens mosquito collected in the valtice area (south moravia, czech republic), as well as representative type strains, were further characterized by ribotyping and whole-cell protein fingerprinting. the ribotype patterns obtained with hindii and ecorv ribotype patterns showed good correlations with the rec ...200819061259
study on lyme borreliosis focus in the lublin region (eastern poland).a suburban focus of lyme borreliosis situated 11 km from the southern border of the city of lublin (eastern poland) was characterized. the focus covers an area of circa 100 km(2), surrounding 3 localities inhabited by circa 7,500 people engaged mostly in farming. it was demonstrated that on the area of focus the infection rate of ixodes ricinus ticks with borrelia burgdorferi, frequency of serological response of inhabitants to the antigen of borrelia burgdorferi, and incidence of lyme borrelios ...200819061271
[borrelial erythema of the face].borrelial infection is characterized by various skin manifestations that are usually classified into three main types: chronic migratory erythema, borrelial lymphocytoma and acrodermatitis chronica atrophicans. we report an unusual case of borrelial cutaneous infection presenting as a mediofacial erythema that cannot be included in any of these three categories.200819084697
a novel and simple method for laboratory diagnosis of relapsing fever borreliosis.relapsing fever caused by borrelia bacteria is often obscured by malaria and incorrectly treated. here a novel method for diagnosis is presented. the method is cheap, simple and requires minimal laboratory material. despite its simplicity, the method shows surprisingly high sensitivity, detecting concentrations less than 10 bacteria/ml blood.200819088905
borrelia antibodies in children evaluated for lyme neuroborreliosis.we wanted to elucidate the value of borrelia antibodies in serum and cerebrospinal fluid (csf) for the diagnosis of lyme neuroborreliosis (lnb).200818791841
production of outer surface protein a by borrelia burgdorferi during transmission from infected mammals to feeding ticks is insufficient to trigger ospa seroconversion.the lyme disease spirochete, borrelia burgdorferi, produces two outer surface lipoproteins, ospa and ospb, that are essential for colonization of tick vectors. both proteins are highly expressed during transmission from infected mammals to feeding ticks and during colonization of tick midguts, but are repressed when bacteria are transmitted from ticks to mammals. humans and other infected mammals generally do not produce antibodies against either protein, although some lyme disease patients do s ...200818793197
relapsing fever borreliosis in interleukin-10-deficient mice.relapsing fever (rf) is a spirochetal infection characterized by periods of sickness with fever at time of high bacteremia that alternate with afebrile periods of relative well being during low bacteremia. patients with epidemic rf who are doing relatively well have extraordinarily high levels of interleukin-10 (il-10) in the circulation. we investigated the possibility that il-10 plays an important protective role in this infection using wild-type and il-10-deficient mice inoculated with virule ...200818794280
vasculitis-like syndrome associated with borrelia lusitaniae infection.we report the isolation of borrelia lusitaniae from a 13-year-old female child presenting with a vasculitis syndrome. the patient was treated with doxycycline, 100 mg bid for 20 days, and is in remission after a follow-up of 2 years. these results should alert clinicians to the fact that b. lusitaniae may be pathogenic in humans, highlighting that patients may be seronegative or present with minimal positive antibody titres and clinical signs that are not specific for lyme borreliosis. in order ...200818795392
prevalence of avian influenza viruses, borrelia garinii, mycobacterium avium, and mycobacterium avium subsp. paratuberculosis in waterfowl and terrestrial birds in slovakia, 2006.the prevalence of borrelia, mycobacteria and avian influenza virus (aiv) infections, together with the distribution of different aiv subtypes, was studied in migratory waterfowl and terrestrial birds trapped in three localities in slovakia during 2006. samples obtained from waterfowl captured in the senianske ponds area of eastern slovakia showed the highest diversity of aiv isolates. a total of 13 different subtypes were detected in 19 samples from this location (h1n2, h2n2, h3n2, h6n6, h7n6, h ...200818798030
two-step synthesis of the immunogenic bacterial glycolipid bbgl1.chemical synthesis of a bacterial glycolipid bbgl1 is reported in two steps starting from per-o-tms d-galactose. the key features are glycosyl iodide mediated beta-stereoselective glycosylation in the absence of neighboring group participation and regioselective acylation.200818798644
retraction. localization of bmpa on the exposed outer membrane of borrelia burgdorferi by monospecific anti-recombinant bmpa rabbit antibodies. 200818799811
interleukin 10 protects the brain microcirculation from spirochetal injury.spirochetal infections are an important cause of neurological disease. in previous studies of the pathogenesis of spirochetal brain infection, mice inoculated with borrelia turicatae, an agent of tick-borne relapsing fever in north america, developed mild meningitis and parenchymal activation/infiltration by interleukin 10 (il-10)-producing microglia/macrophages. here, we investigated the neuroprotective effects of il-10 during spirochetal infection by comparing the outcomes of b. turicatae infe ...200818800010
clinical predictors of lyme disease among children with a peripheral facial palsy at an emergency department in a lyme disease-endemic area.although lyme disease can cause peripheral facial palsy in lyme disease-endemic areas, diagnostic predictors in children have not been described.200818931349
evaluation of the recombinant vlse-based liaison chemiluminescence immunoassay for detection of borrelia burgdorferi and diagnosis of lyme disease.recent efforts to improve the serologic diagnosis of lyme disease have included the use of a synthetic peptide (c6) that reproduces the sequence of invariable region 6 of vlse, the variable surface antigen of borrelia burgdorferi. in the present study, the diagnostic performance of diasorin's recombinant vlse-based chemiluminescence immunoassay in 1,947 human serum samples was evaluated. sensitivity was determined using two serum panels from the cdc. for panel i, we observed sensitivities of 68. ...200818945880
retrospective clinical and molecular analysis of conditioned laboratory dogs (canis familiaris) with serologic reactions to ehrlichia canis, borrelia burgdorferi, and rickettsia rickettsii.dogs are susceptible to different tickborne infections, including members of the anaplasmataceae (ehrlichia canis, e. ewingii, e. chaffeensis, anaplasma phagocytophilum, a. platys), borrelia burgdorferi, and rickettsia rickettsii. these diseases can manifest with clinical signs including fever, anorexia, malaise, lameness, rash, and bleeding episodes; however, these signs are nonpathognomonic, and infections can occur in the absence of clinical signs. hematologic abnormalities can include leukop ...200818947166
percent positive rate of lyme real-time polymerase chain reaction in blood, cerebrospinal fluid, synovial fluid, and tissue.we performed a retrospective review of 23,777 specimens tested by our lyme real-time polymerase chain reaction assay to determine the percent positive rates by specimen source. the percent positive rates were highest in synovial fluid (6.4%) and tissue (6.5%), and lowest in blood (0.1%) and cerebrospinal fluid (0.09%).200818947959
borrelia burgdorferi lacking dbpba exhibits an early survival defect during experimental infection.several borrelia burgdorferi genes induced under mammalian host conditions have been purported to be important in lyme disease pathogenesis based on their binding to host structures. these genes include the dbpba locus, whose products bind host decorin and glycosoaminoglycans. recently, the dbpba genes were reported to be involved in borrelial infectivity. here we extended the previous observations by using culture and quantitative pcr to evaluate low- and high-dose murine infection by a delta d ...200818809667
cerebral vasculitis and stroke in lyme neuroborreliosis. two case reports and review of current knowledge.we report on 2 patients with cerebral vasculitis and stroke due to lyme neuroborreliosis (lnb). both patients had a prodromal stage involving headaches, and showed meningeal enhancement in addition to ischemic infarctions on brain magnetic resonance imaging and diffuse vasculitis on vascular imaging. serological and cerebrospinal (csf) fluid studies confirmed the diagnosis of active lnb. ceftriaxone for 3 weeks led to an excellent recovery and improvements in the csf examination findings. stroke ...200818810231
borrelia burgdorferi sensu lato, the agent of lyme borreliosis: life in the wilds.in europe, borrelia burgdorferi sensu lato (sl) the agent of lyme borreliosis circulates in endemic areas between ixodes ricinus ticks and a large number of vertebrate hosts upon which ticks feed. currently, at least 12 different borrelia species belonging to the complex b. burgdorferi sl have been identified among which seven have been detected in i. ricinus: b. burgdorferi sensu stricto (ss), b. garinii, b. afzelii, b. valaisiana, b. spielmanii and b. bissettii. a few dozens of vertebrate host ...200818814688
persisting atypical and cystic forms of borrelia burgdorferi and local inflammation in lyme neuroborreliosis.the long latent stage seen in syphilis, followed by chronic central nervous system infection and inflammation, can be explained by the persistence of atypical cystic and granular forms of treponema pallidum. we investigated whether a similar situation may occur in lyme neuroborreliosis.200818817547
primary cutaneous marginal zone b-cell lymphoma: a molecular and clinicopathological study of cases from asia, germany, and the united states.primary cutaneous marginal zone b-cell lymphoma is considered the cutaneous counterpart of extranodal marginal zone b-cell lymphoma of mucosa-associated lymphoid tissue. although its molecular pathogenesis is currently unknown, an etiological link with borrelia burgdorferi infection has been identified in european, but not in american or asian cases. to better understand the pathogenesis and the geographical differences of cutaneous marginal zone b-cell lymphoma, 60 cases from the east asia, ger ...200818820662
frequency of antibodies to babesia bigemina, b. bovis, anaplasma marginale, trypanosoma vivax and borrelia burgdorferi in cattle from the northeastern region of the state of pará, brazil.babesiosis, anaplasmosis, and trypanosomosis are relevant diseases, potentially causing morbidity in cattle, leading to economic losses. borreliosis is import as a potential zoonosis. the objective of this study was to determine, by indirect enzyme-linked immunosorbent assay (elisa), the frequency of seropositive cattle to babesia bigemina, b. bovis, anaplasma marginale, trypanosoma vivax and borrelia burgdorferi in cattle from the northeastern region of pará, brazil. sera samples from 246 femal ...200818823579
adenoviral delivery of interleukin-10 fails to attenuate experimental lyme disease.production of interleukin-10 (il-10) by c57bl/6 mice following infection with borrelia burgdorferi has been proposed as a mechanism whereby resistance to the development of experimental lyme arthritis is maintained. in the current study, we sought to determine the role of il-10 during infection of arthritis- and carditis-susceptible c3h mice. infection of c3h il-10(-/-) mice led to increased joint swelling and arthritis severity scores over those of wild-type c3h mice. measurement of b. burgdorf ...200818824530
deciphering the ligand-binding sites in the borrelia burgdorferi complement regulator-acquiring surface protein 2 required for interactions with the human immune regulators factor h and factor h-like protein 1.borrelia burgdorferi, the etiologic agent of lyme disease, employs sophisticated means to evade killing by its mammalian hosts. one important immune escape mechanism is the inhibition of complement activation mediated by interactions of the host-derived immune regulators factor h (cfh) and factor h-like protein 1 (cfhl1) with borrelial complement regulator-acquiring surface proteins (bbcrasps). bbcrasp-2 is a distinctive cfh- and cfhl1-binding protein that is produced by serum-resistant b. burgd ...200818824548
[small risk of developing lyme borreliosis following a tick bite on ameland: research in a general practice].to investigate the percentage of ticks infected with borrelia burgdorferi on the dutch north sea island of ameland, and the risk of developing lyme disease following tick bite on the island.200818825891
[characteristic features of the ranks genus and subgenus, and an intercalary rank "species complex" in ixodid ticks (acari: ixodidae)].the work was carries our from the standpoint of the morphological conception of species. vast collections of the zoological institute of the russian academy of science testify to the existence of hiatuses in both genera and subgenera of palearctic ixodids at all active phases of their ontogenesis. the fact that the subgenera of palearctic genera have been well studies is notes, and composition of the subgenera is presented. the question of a taxonomic intercalary rank "species complex" is consid ...200818825916
internal core protein cleavage leaves the hepatitis b virus capsid intact and enhances its capacity for surface display of heterologous whole chain proteins.virus capsids find increasing use as nanoparticulate platforms for the surface display of heterologous ligands, including as multivalent vaccine carriers. presentation on the icosahedral hepatitis b virus capsid (hbcag) is known to strongly enhance immunogenicity of foreign sequences, most efficiently if they are inserted into the dominant c/e1 b cell epitope, a surface-exposed loop in the center of the constituent core protein primary sequence. even some complete proteins were successfully inse ...200818826949
then and now: use of 16s rdna gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories.in the last decade, as a result of the widespread use of pcr and dna sequencing, 16s rdna sequencing has played a pivotal role in the accurate identification of bacterial isolates and the discovery of novel bacteria in clinical microbiology laboratories. for bacterial identification, 16s rdna sequencing is particularly important in the case of bacteria with unusual phenotypic profiles, rare bacteria, slow-growing bacteria, uncultivable bacteria and culture-negative infections. not only has it pr ...200818828852
surveillance for lyme disease--united states, 1992-2006.lyme disease is a multisystem disease that occurs in north america, europe, and asia. in the united states, the etiologic agent is borrelia burgdorferi sensu stricto, a spirochete transmitted to humans by infected ixodes scapularis and i. pacificus ticks. the majority of patients with lyme disease develop a characteristic rash, erythema migrans (em), accompanied by symptoms of fever, malaise, fatigue, headache, myalgia, or arthralgia. other manifestations of infection can include arthritis, card ...200818830214
interaction of the lyme disease spirochete borrelia burgdorferi with brain parenchyma elicits inflammatory mediators from glial cells as well as glial and neuronal apoptosis.lyme neuroborreliosis, caused by the spirochete borrelia burgdorferi, often manifests by causing neurocognitive deficits. as a possible mechanism for lyme neuroborreliosis, we hypothesized that b. burgdorferi induces the production of inflammatory mediators in the central nervous system with concomitant neuronal and/or glial apoptosis. to test our hypothesis, we constructed an ex vivo model that consisted of freshly collected slices from brain cortex of a rhesus macaque and allowed live b. burgd ...200818832582
molecular mechanisms involved in vascular interactions of the lyme disease pathogen in a living host.hematogenous dissemination is important for infection by many bacterial pathogens, but is poorly understood because of the inability to directly observe this process in living hosts at the single cell level. all disseminating pathogens must tether to the host endothelium despite significant shear forces caused by blood flow. however, the molecules that mediate tethering interactions have not been identified for any bacterial pathogen except e. coli, which tethers to host cells via a specialized ...200818833295
common and unique contributions of decorin-binding proteins a and b to the overall virulence of borrelia burgdorferi.as an extracellular bacterium, the lyme disease spirochete borrelia burgdorferi resides primarily in the extracellular matrix and connective tissues and between host cells during mammalian infection, where decorin and glycosaminoglycans are abundantly found, so its interactions with these host ligands potentially affect various aspects of infection. decorin-binding proteins (dbps) a and b, encoded by a 2-gene operon, are outer surface lipoproteins with similar molecular weights and share approxi ...200818833332
variations in ixodes ricinus density and borrelia infections associated with cattle introduced into a woodland in the netherlands.the effect of introduced large herbivores on the abundance of ixodes ricinus ticks and their borrelia infections was studied in a natural woodland in the netherlands. oak and pine plots, either ungrazed or grazed by cattle, were selected. ticks were collected weekly by blanket dragging. borrelia infections were determined by pcr and restriction fragment length polymorphism. rodent densities were estimated using mark-release-recapture methods. on occasion, the cattle were inspected for tick infes ...200818836006
identifying the reservoir hosts of the lyme disease spirochete borrelia burgdorferi in california: the role of the western gray squirrel (sciurus griseus).we investigated the role of the western gray squirrel (sciurus griseus) as a reservoir host of the lyme disease spirochete borrelia burgdorferi. a survey of 222 western gray squirrels in california showed an overall prevalence of b. burgdorferi infection of 30%, although at a county level, prevalence of infection ranged from 0% to 50% by polymerase chain reaction. laboratory trials with wild-caught western gray squirrels indicated that squirrels were competent reservoir hosts of the lyme disease ...200818840740
the promiscuity of beta-strand pairing allows for rational design of beta-sheet face inversion.recent studies suggest the dominant role of main-chain h-bond formation in specifying beta-sheet topology. its essentially sequence-independent nature implies a large degree of freedom in designing beta-sheet-based nanomaterials. here we show rational design of beta-sheet face inversions by incremental deletions of beta-strands from the single-layer beta-sheet of borrelia outer surface protein a. we show that a beta-sheet structure can be maintained when a large number of native contacts are rem ...200818842042
cross-reaction between yersinia outer membrane proteins and anti-borrelia antibodies in sera of patients with lyme disease.diagnosis of yersinia infections accompanied by reactive arthritis could be complicated by cross-reaction with other arthritogenic bacteria. the possible cross-reaction between yersinia antigens and anti-borrelia antibodies in blood sera of patients with lyme disease was studied. the occurrence of specific iga, igg and igm antibodies was analyzed in serum samples from 30 patients with yersinia-triggered reactive arthritis, 30 patients with lyme disease and five samples from healthy blood donors. ...200818844689
borrelia burgdorferi expression of the bba64, bba65, bba66, and bba73 genes in tissues during persistent infection in mice.borrelia burgdorferi, the etiological agent of lyme disease in humans, is vectored between mammalian hosts in nature by ixodes ticks. the organism adapts to diverse environments encountered throughout the enzootic cycle by differentially expressing essential gene products to survive the specialized conditions, whether in ticks or warm-blooded hosts. however, little is known regarding the identity and/or function of b. burgdorferi genes expressed during colonization of tissues during mammalian in ...200818848981
in vitro cpg methylation increases the transformation efficiency of borrelia burgdorferi strains harboring the endogenous linear plasmid lp56.borrelia burgdorferi is the causative agent of lyme disease, the most common vector-borne illness in the northern hemisphere. low-passage-number infectious strains of b. burgdorferi exhibit extremely low transformation efficiencies-so low, in fact, as to hinder the genetic study of putative virulence factors. two putative restriction-modification (r-m) systems, bbe02 contained on linear plasmid 25 (lp25) and bbq67 contained on lp56, have been postulated to contribute to this poor transformabilit ...200818849429
borrelia burgdorferi genotype predicts the capacity for hematogenous dissemination during early lyme disease.lyme disease, the most common tickborne disease in the united states, is caused exclusively by borrelia burgdorferi sensu stricto in north america. the present study evaluated the genotypes of >400 clinical isolates of b. burgdorferi recovered from patients from suburban new york city with early lyme disease associated with erythema migrans; it is the largest number of borrelial strains from north america ever to be investigated.200818781866
the genome of borrelia recurrentis, the agent of deadly louse-borne relapsing fever, is a degraded subset of tick-borne borrelia duttonii.in an effort to understand how a tick-borne pathogen adapts to the body louse, we sequenced and compared the genomes of the recurrent fever agents borrelia recurrentis and b. duttonii. the 1,242,163-1,574,910-bp fragmented genomes of b. recurrentis and b. duttonii contain a unique 23-kb linear plasmid. this linear plasmid exhibits a large polyt track within the promoter region of an intact variable large protein gene and a telomere resolvase that is unique to borrelia. the genome content is char ...200818787695
spatial dynamics of lyme disease: a review.lyme disease (ld), the most frequently reported vector-borne disease in the united states, requires that humans, infected vector ticks, and infected hosts all occur in close spatial proximity. understanding the spatial dynamics of ld requires an understanding of the spatial determinants of each of these organisms. we review the literature on spatial patterns and environmental correlates of human cases of ld and the vector ticks, ixodes scapularis in the northeastern and midwestern united states ...200818787920
meta-analysis of coinfection and coexposure with borrelia burgdorferi and anaplasma phagocytophilum in humans, domestic animals, wildlife, and ixodes ricinus-complex ticks.anaplasma phagocytophilum, which causes granulocytic anaplasmosis, and borrelia burgdorferi, the agent of lyme borreliosis, are transmitted in multiple holarctic regions by the same ixodes sp. tick vectors and maintained in sylvatic cycles with the same rodent reservoirs. coinfection of humans, domestic animals, wildlife, and ticks with both b. burgdorferi and a. phagocytophilum appears to be common, yet the pathologic mechanisms and ecology remain poorly understood compared with single-agent in ...200818789001
protein binding has a large effect on radical mediated dna damage.oxidative dna damage is important in aging and a variety of diseases. significant advances have been made in our understanding of the chemistry of radical mediated dna damage. these studies have been carried out on dna in the absence of proteins. however, in cells dna is typically bound by proteins such as in chromatin and transiently by proteins that regulate biochemical processes. how and whether protein binding affects dna radical reactivity is not well understood. the effect of the dna bindi ...200818778053
outer surface protein a protects lyme disease spirochetes from acquired host immunity in the tick vector.the lyme disease spirochete borrelia burgdorferi alters the expression of outer surface protein (osp) genes as the bacterium cycles between ticks and mammals. ospa is produced as borreliae enter the tick vector and remains a major surface antigen during midgut colonization. to elucidate the role of ospa in the vector, we created an insertional deletion of ospa in strain b31-a3. the ospa mutant infects mice when it is injected intradermally and is acquired by larval ticks fed on these mice, where ...200818779341
deletion of bba64, bba65, and bba66 loci does not alter the infectivity of borrelia burgdorferi in the murine model of lyme disease.borrelia burgdorferi, the causative agent of lyme disease, alters its gene expression in response to highly disparate environmental signals encountered in its tick vector versus vertebrate hosts. whole-genome transcriptional profile analysis of b. burgdorferi, propagated in vitro under mammalian-host-specific conditions, revealed significant upregulation of several linear plasmid 54 (lp54)-encoded open reading frames (orfs). among these orfs, bba64, bba65, and bba66 have been shown to be upregul ...200818765733
reccurent erythema migrans as a persistent infection.effective antibiotic therapy administered early in the course of the clinical presentation of erythema migrans (em) prevents further progression of infection and dissemination of the agent to internal organs.200818767376
lack of evidence for borrelia burgdorferi seropositivity in alzheimer disease. 200818769174
selenium status and antibodies to selected pathogens in white-tailed deer (odocoileus virginianus) in southern minnesota.to determine exposure to a variety of infectious diseases potentially important for native ungulates, livestock, and humans, serum samples from 114 (94 adults, 20 fawns) female white-tailed deer (odocoileus virginianus) were collected during january 2000-03 from multiple locations in southeast (se) and southwest (sw) minnesota. antibody prevalence was determined for the following pathogens: mycobacterium avium subsp. paratuberculosis, leptospira interrogans (six serovars), anaplasma marginale, b ...200818263838
complement split products c3a and c4a are early markers of acute lyme disease in tick bite patients in the united states.current laboratory markers do not readily detect acute lyme disease. we assessed the utility of complement and its split products as markers of lyme disease in patients shortly after a tick bite.200818270493
tick infestation risk and borrelia burgdorferi s.l. infection-induced increase in host-finding efficacy of female ixodes ricinus under natural conditions.an investigation of the risk of human tick infestation, together with the prevalence of borrelia burgdorferi s.l. infection, was conducted in a sylvatic habitat in western germany to provide data needed for future risk-benefit evaluations of acaricides used for clothing impregnation. additionally, data were collected on behavioural changes in borrelia burgdorferi s.l.-infected adult female i. ricinus ticks and the possible impact of such changes on host-finding efficacy. the risk of i. ricinus-i ...200818273686
[seroprevalence of lyme borreliosis and tick-borne encephalitis in workers at risk, in eastern france].the aim of this article was to assess the seroprevalence of lyme borreliosis and tick-borne encephalitis (tbe) among occupationally exposed forest workers.200818722066
borrelia burgdorferi complement regulator-acquiring surface protein 2 does not contribute to complement resistance or host infectivity.borrelia burgdorferi, the pathogen of lyme disease, cycles in nature through ixodes ticks and mammalian hosts. at least five complement regulator-acquiring surface proteins (bbcrasps) are produced by b. burgdorferi, which are thought to assist spirochetes in host immune evasion. recent studies established that bbcrasp-2 is preferentially expressed in mammals, and elicits robust antibody response in infected hosts, including humans. we show that bbcrasp-2 is ubiquitously expressed in diverse muri ...200818714378
[lyme disease--case report].the authors presents a female case of lyme disease. there are presented types of onset, evolution and treatment in this case.200818714491
transmission dynamics of borrelia burgdorferi s.s. during the key third day of feeding by nymphal ixodes scapularis (acari: ixodidae).nymphal ixodes scapularis say are the principal vectors of lyme disease spirochetes (borrelia burgdorferi sensu stricto) in the eastern united states. physicians frequently face the decision of whether or not to administer prophylactic antibiotics to human tick bite victims in lyme disease endemic regions, based on the overall probability that such bites will result in infection with b. burgdorferi s.s. we evaluated the transmission dynamics of b. burgdorferi s.s. during the key third day of nym ...200818714875
dispelling the chronic lyme disease myth.lyme disease is a tick-borne illness endemic to minnesota that can have potentially severe complications. as the incidence of lyme disease continues to increase, it is important for physicians in minnesota to become familiar with its clinical aspects, including the concept of "chronic lyme disease." chronic lyme disease is a misnomer that is often applied to patients with nonspecific presentations who may or may not have a history of infection with borrelia burgdorferi, the agent that causes lym ...200818714930
impact of clinical variables on borrelia burgdorferi-specific antibody seropositivity in acute-phase sera from patients in north america with culture-confirmed early lyme disease.erythema migrans, the most common manifestation of lyme disease, has been associated with highly variable rates of seropositivity for antibodies to borrelia burgdorferi. differences in the sensitivities of serologic assays for the detection of these antibodies, however, may not be the only or even the primary explanation for this observation. we investigated the impacts of four clinical variables on seropositivity--the duration of erythema migrans, the presence of single versus multiple skin les ...200818716009
validation of cultivation and pcr methods for diagnosis of lyme neuroborreliosis.borrelial infection may manifest with a wide range of clinical signs, and in many cases, microbiological findings are essential for a proper diagnosis. this study included 48 patients with a working clinical diagnosis of lyme neuroborreliosis, 45 patients with a working clinical diagnosis of suspected lyme neuroborreliosis, and a control group comprising 42 patients with tick-borne encephalitis and 21 neurosurgical patients. the aim of the study was to analyze and compare findings of two pcr met ...200818716226
microbial pathogens in ticks, rodents and a shrew in northern gyeonggi-do near the dmz, korea.a total of 1,618 ticks [420 individual (adults) and pooled (larvae and nymphs) samples], 369 rodents (apodemus agrarius, rattus norvegicus, tscherskia triton, mus musculus, and myodes regulus), and 34 shrews (crocidura lasiura) that were collected in northern gyeonggi-do near the demilitarized zone (dmz) of korea during 2004-2005, were assayed by pcr for selected zoonotic pathogens. from a total of 420 individual and pooled tick dna samples, anaplasma (a.) phagocytophilum (16), a. platys (16), e ...200818716449
localized abdominal idiopathic lipodystrophy.localized loss of subcutaneous tissue can occur after panniculitis, injections of corticosteroids and other drugs, or associated with infectious, autoimmune or neurologic diseases. the "idiopathic lipoatrophies" are a group of poorly characterized diseases, with focal disappearance of subcutaneous fat, and usually the thighs, abdomen or the ankles are affected. three subtypes have been described based on clinical presentation: lipoatrophia semicircularis, annular lipoatrophy of the ankles and ce ...200818718199
neuroborreliosis recurrence: reinfection or relapse?we report the case of a 47-y-old female with documented neuroborreliosis, who had a complete recovery after 10 d of intravenous high-dose penicillin followed after 9 months by a new episode of documented neuroborreliosis. the case probably represents a rare case of true reinfection rather than relapse.200818720254
effect of borrelia burgdorferi genotype on the sensitivity of c6 and 2-tier testing in north american patients with culture-confirmed lyme disease.a potential concern with any serologic test to detect antibodies to borrelia burgdorferi is whether the epitopes incorporated in the test provide sufficient cross-reactivity to detect infection with all of the pathogenic strains of the species. this is a particular concern for the c6 test, which is based on reactivity to a single peptide.200818724824
borrelia burgdorferi lipoprotein bmpa activates pro-inflammatory responses in human synovial cells through a protein moiety.borrelia burgdorferi invasion of mammalian joints results in genesis of lyme arthritis. other than spirochete lipids, existence of protein antigens, which are abundant in joints and participate in b. burgdorferi-induced host inflammatory response, is unknown. here, we report that major products of the b. burgdorferi basic membrane protein (bmp) a/b operon that are induced in murine and human joints, possess inflammatory properties. compared to the wild type b. burgdorferi, an isogenic bmpa/b mut ...200818725314
serologic evidence of anaplasma phagocytophilum infections in patients with a history of tick bite in central slovakia.the aim of this study was to determine the risks of human anaplasmosis in an area of central slovakia endemic for lyme borreliosis. the circulation of anaplasma phagocytophilum in ticks and wild animals has been observed in natural foci in this area for several years. samples of human sera from patients with lyme borreliosis and persons with a history of recent tick bite and clinical symptoms indicating lyme borreliosis were collected in central slovakia. a total of 76 human sera were analyzed u ...200818726669
no association of seropositivity for anti-borrelia igg antibody with mental and physical complaints.undiagnosed chronic lyme disease caused by borrelia burgdorferi is considered a differential diagnoses in medically unexplained symptoms like arthralgias, distal paresthesias, depressive symptoms, lack of concentration and fatigue. the aims of the study were to assess the association of mental and physical complaints with seropositivity for anti-borrelia igg in a general population sample. seropositivity indicated an infection with borrelia in the past. the study of health in pomerania was condu ...200818752103
preferential protection of borrelia burgdorferi sensu stricto by a salp15 homologue in ixodes ricinus saliva.ixodes ticks are the main vectors for borrelia burgdorferi sensu lato. in the united states, b. burgdorferi is the sole causative agent of lyme borreliosis and is transmitted by ixodes scapularis. in europe, 3 borrelia species-b. burgdorferi, b. garinii, and b. afzelii-are prevalent, which are transmitted by ixodes ricinus. the i. scapularis salivary protein salp15 has been shown to bind to b. burgdorferi outer surface protein (osp) c, protecting the spirochete from antibody-mediated killing.200818752445
rapid detection and identification of a pathogen's dna using phi29 dna polymerase.zoonotic pathogens including those transmitted by insect vectors are some of the most deadly of all infectious diseases known to mankind. a number of these agents have been further weaponized and are widely recognized as being potentially significant biothreat agents. we describe a novel method based on multiply-primed rolling circle in vitro amplification for profiling genomic dnas to permit rapid, cultivation-free differential detection and identification of circular plasmids in infectious age ...200818755142
tick-borne relapsing fever.each year, many residents of and visitors to endemic regions of the western united states are exposed to the tick vectors of tick-borne relapsing fever (tbrf), ornithodoros hermsi, ornithodoros turicata, or ornithodoros parkeri. this disease is remarkable because the human host is unaware of the tick bite, usually becomes very ill, may experience an exacerbation of symptoms rather than improvement shortly after beginning appropriate treatment, and, despite often high numbers of the etiologic org ...200818755384
[combined method for testing microflora in oral specimens].the integrated procedure used in a clinical laboratory to test oral specimens for the microflora, which includes microscopic and rapid cultural studies, enhances the specificity and sensitivity of detection of fungi of the genus candida as the used chromogenic selective nutrient medium allows c. albicans, c. glabrata, and c. tropocalis to be identified in the primary culture, without increase the time of giving the results of a test. the results of a test for the fungi may be quantitatively pres ...200818756737
oms38 is the first identified pore-forming protein in the outer membrane of relapsing fever spirochetes.relapsing fever is a worldwide, endemic disease caused by several spirochetal species belonging to the genus borrelia. during the recurring fever peaks, borreliae proliferate remarkably quickly compared to the slow dissemination of lyme disease borrelia and therefore require efficient nutrient uptake from the blood of their hosts. this study describes the identification and characterization of the first relapsing fever porin, which is present in the outer membranes of b. duttonii, b. hermsii, b. ...200818757545
transcriptional interplay among the regulators rrp2, rpon and rpos in borrelia burgdorferi.the rpon-rpos alternative sigma factor pathway is essential for key adaptive responses by borrelia burgdorferi, particularly those involved in the infection of a mammalian host. a putative response regulator, rrp2, is ostensibly required for activation of the rpon-dependent transcription of rpos. however, questions remain regarding the extent to which the three major constituents of this pathway (rrp2, rpon and rpos) act interdependently. to assess the functional interplay between rrp2, rpon and ...200818757798
prevalence of tick-borne zoonotic bacteria in questing adult ticks from northern spain.a total of 691 questing adult ixodid ticks of the genera ixodes, haemaphysalis, dermacentor, and rhipicephalus were tested by polymerase chain reaction (pcr) and reverse line blot (rlb) for the presence of anaplasma phagocytophilum, coxiella burnetii, borrelia spp., and spotted fever group (sfg) rickettsiae. ticks were collected by blanket dragging during 2 sampling years (2003-2005) in 10 recreational areas in the basque country (northern spain). adult ticks were collected every month of the ye ...200818759563
[cross-reaction evaluation of pcr-anaplasma platys positive dogs tested to anaplasma phagocytophilum antibodies by commercial elisa].anaplasma platys, agent of canine cyclic thrombocytopenia parasites exclusively dogs platelets. its probable vector is the tick rhipicephalus sanguineus. among the existent diagnostic methods, the most used include: morulae identification in blood smears; antibody detection by indirect immunofluorescence; or dna amplification by polymerase chain reaction (pcr). recently a new commercial elisa (idexx®), capable of detecting a. phagocytophilum antibodies, has been developed. according to the manuf ...200820059806
a longitudinal study of the prevalence of borreliae in ticks in the urban locality of brno-pisárky, czech republic. 200819263859
[spatial and temporal variability of ixodes ricinus and ixodes persulcatus infection with the lyme disease agent in moscow region].analysis of long-term data on the infection rated of the taxonomic complex borrelia burgdorferi s. l. in ixodes persulcatus and i. ricinus from moscow region is carried out. more than 14 000 tick specimens were examined by dark-field microscopy and 704 specimens were investigated by pcr. two borrelia genospicies, b. afzelii (6.3%) and b. garinii (20%) proved the most prevalent. the genospecies could form a mixtinfection (11%). borrelia miyamotoi, b. valaisiana, and b. burgdorferi s. s. were also ...200819198169
microbial communities and interactions in the lone star tick, amblyomma americanum.to quantify microbial composition and interactions, we identified prokaryotic communities in the lone star tick (amblyomma americanum) based on 16s rrna gene sequences and direct probing. the lone star tick is the vector of emerging diseases and host to additional symbionts of unknown activity, and is representative of other blood-sucking arthropods. we evaluated the potential for vertical (transovarial) transmission by molecular analysis of microbial symbionts from egg and larval clutches. dire ...200819378409
[radiculalgia revealing lyme disease]. 200819213505
[results of etiologic diagnosis in clinical syndrome consistent with acute and chronic borreliosis].borreliosis is a multisystem infection, which in the absence of adequate diagnosis and clinical management, may develop towards various clinical forms of chronic pathology. due to the heterogeneity of clinical manifestations it is known under more names: erythema migrans, lyme disease, neuroborreliosis etc. material and method: taking into account the present interest and the weight in pathology of syndromes consistent with the suspicion of a borrelia spp. infection, since 2002 we applied in cur ...200819295026
[retinal manifestations of infectious diseases].the retina and the choroids are richly vascularised structures and can therefore be colonised by germs via the haematogenous route in the course of a systemic infectious disease. the germs responsible for this type of infection can be fungi, viruses, bacteria and parasites. ocular candidiasis is outstanding amongst these colonisations because of its frequency; it can manifest itself as an endophthalmitis with a slow and hidden course. the so-called ocular histoplasmosis syndrome, although it is ...200819169295
lyme disease. 200819170319
subacute transverse myelitis with lyme profile dissociation.transverse myelitis is a very rare neurologic syndrome with an incidence per year of 1-5 per million population. we are presenting an interesting case of subacute transverse myelitis with its mri (magnetic resonance imaging) and csf (cerebrospinal fluid) findings.200819675732
holliday junction formation by the borrelia burgdorferi telomere resolvase, rest: implications for the origin of genome linearity.spirochetes of the genus borrelia include the causative agents of lyme disease and relapsing fever. they possess unusual, highly segmented genomes composed mostly of linear replicons with covalently closed hairpin telomeres. the telomeres are formed from inverted repeat replicated telomere junctions (rtels) by the telomere resolvase, rest. rest uses a reaction mechanism with similarities to that employed by the type ib topoisomerases and tyrosine recombinases. here, we report that the relationsh ...200919170885
identification of anaplasma phagocytophilum and borrelia burgdorferi sensu lato in patients with erythema migrans.anaplasma phagocytophilum has been first isolated from the blood of two czech patients simultaneously with a cultivation of borrelia burgdorferi sensu lato from their erythema migrans lesions. cultivation of different borrelia spp. from 12 erythema migrans biopsies, from 2 blood, one liquor and one placenta sample in bsk-h medium was successful. adapted conventional methods targeting 16s rrna and ospa genes for real-time polymerase chain reaction (pcr) and partial sequencing of these genes toget ...200919649743
community-based prevention of lyme disease and other tick-borne diseases through topical application of acaricide to white-tailed deer: background and rationale.this series of articles describes the first large-scale experiment designed to explore the efficacy of reducing the risk of tick-borne disease in highly endemic communities of the northeastern and mid-atlantic united states through deployment of a self-application device that treats white-tailed deer with acaricide to prevent feeding by adult ixodes scapularis ticks and all stages of amblyomma americanum ticks where both species occur. the results of the multicenter study are reported in the acc ...200919650729
topical treatment of white-tailed deer with an acaricide for the control of ixodes scapularis (acari: ixodidae) in a connecticut lyme borreliosis hyperendemic community.the 4-poster device for the topical treatment of white-tailed deer, odocoileus virginianus (zimmermann), against ticks using the acaricide amitraz, was evaluated in a lyme borreliosis endemic community in connecticut. as part of a 5-year project from 1997 to 2002, 21-24 of the 4-posters were distributed at residential sites in old lyme, ct, in a core treatment area of approximately 5.2 km(2) in fall 1997. the 4-posters were active october to mid-december and march into may, corresponding to the ...200919650731
effects of tick control by acaricide self-treatment of white-tailed deer on host-seeking tick infection prevalence and entomologic risk for ixodes scapularis-borne pathogens.we evaluated the effects of tick control by acaricide self-treatment of white-tailed deer on the infection prevalence and entomologic risk for three ixodes scapularis-borne bacteria in host-seeking ticks. ticks were collected from vegetation in areas treated with the "4-poster" device and from control areas over a 6-year period in five geographically diverse study locations in the northeastern united states and tested for infection with two known agents of human disease, borrelia burgdorferi and ...200919650738
borrelia burgdorferi resistance to a major skin antimicrobial peptide is independent of outer surface lipoprotein content.we hypothesize a potential role for borrelia burgdorferi ospc in innate immune evasion at the initial stage of mammalian infection. we demonstrate that b. burgdorferi is resistant to high levels (>200 microg/ml) of cathelicidin and that this antimicrobial peptide exhibits limited binding to the spirochetal outer membrane, irrespective of ospc or other abundant surface lipoproteins. we conclude that the essential role of ospc is unrelated to resistance to this component of innate immunity.200919651916
guaa and guab are essential for borrelia burgdorferi survival in the tick-mouse infection cycle.pathogens lacking the enzymatic pathways for de novo purine biosynthesis are required to salvage purines and pyrimidines from the host environment for synthesis of dna and rna. two key enzymes in purine salvage pathways are imp dehydrogenase (guab) and gmp synthase (guaa), encoded by the guab and guaa genes, respectively. while these genes are typically found on the chromosome in most bacterial pathogens, the guaab operon of borrelia burgdorferi is present on plasmid cp26, which also harbors a n ...200919666713
a novel fibronectin binding motif in mscramms targets f3 modules.bbk32 is a surface expressed lipoprotein and fibronectin (fn)-binding microbial surface component recognizing adhesive matrix molecule (mscramm) of borrelia burgdorferi, the causative agent of lyme disease. previous studies from our group showed that bbk32 is a virulence factor in experimental lyme disease and located the fn-binding region to residues 21-205 of the lipoprotein.200919404402
the epidemiology of tick-borne relapsing fever in iran during 1997-2006.tick-borne relapsing fever is an acute febrile and endemic disease in iran. for many reasons, the incidence of disease is on decrease, however tick-borne relapsing fever is still a health issue in the rural areas for travelers. this study was carried out during 1997-2006 to investigate the tick-borne relapsing fever in iran.200919411042
levels of svcam-1 and sicam-1 in patients with lyme disease.lyme disease is a multi-organ animal-borne disease caused by the spirochete borrelia burgdorferi (bb).200919413177
ticks and tick-borne pathogens and putative symbionts of black bears (ursus americanus floridanus) from georgia and florida.ticks were collected from 38 black bears (ursus americanus floridanus) from northwestern florida (n = 18) from 2003 to 2005 and southern georgia (n = 20) in 2006. five species (amblyomma americanum, a. maculatum, dermacentor variabilis, ixodes scapularis, and i. affinis) were collected from florida bears, and 4 species (a. americanum, a. maculatum, d. variabilis, i. scapularis) were collected from bears in georgia. ixodes scapularis was the most frequently collected tick, followed by d. variabil ...200919413369
concomitant human granulocytic anaplasmosis and lyme neuroborreliosis. 200919416290
cerebrospinal fluid pcr and antibody concentrations against anaplasma phagocytophilum and borrelia burgdorferi sensu lato in dogs with neurological signs.the tick-borne bacteria borrelia burgdorferi sensu lato (sl) and anaplasma phagocytophilum have been suspected to cause neurological signs in dogs. diagnosis often has been made based on positive antibody titers in serum of dogs with neurological signs, but a high seroprevalence in dogs in at-risk populations makes diagnosis difficult.200919422467
follow-up of bernese mountain dogs and other dogs with serologically diagnosed borrelia burgdorferi infection: what happens to seropositive animals?data on the long-term outcome of b. burgdorferi infections in adult dogs are sparse. the aim of the present study was to investigate whether bernese mountain dogs with serological evidence of natural b. burgdorferi infection more often develop signs such as lameness, azotemia or proteinuria during a follow-up period of 2.5 to 3.0 years. seropositive bernese mountain dogs were compared to seronegative bernese mountain dogs and to seropositive and seronegative control dogs of other breeds. dogs in ...200919426490
intact flagellar motor of borrelia burgdorferi revealed by cryo-electron tomography: evidence for stator ring curvature and rotor/c-ring assembly flexion.the bacterial flagellar motor is a remarkable nanomachine that provides motility through flagellar rotation. prior structural studies have revealed the stunning complexity of the purified rotor and c-ring assemblies from flagellar motors. in this study, we used high-throughput cryo-electron tomography and image analysis of intact borrelia burgdorferi to produce a three-dimensional (3-d) model of the in situ flagellar motor without imposing rotational symmetry. structural details of b. burgdorfer ...200919429612
development of a single-plasmid-based regulatable gene expression system for borrelia burgdorferi.we developed a single-plasmid-based regulatable protein expression system for borrelia burgdorferi. expression of a target gene is driven by p(ost), a hybrid b. burgdorferi ospa-teto promoter, from a recombinant b. burgdorferi plasmid constitutively expressing tetr. the system was tested using the green fluorescent protein (gfp) as a reporter. under noninducing conditions, recombinant b. burgdorferi cells were nonfluorescent, no gfp protein was detected, and residual, small amounts of transcript ...200919700541
borrelia burgdorferi small lipoprotein lp6.6 is a member of multiple protein complexes in the outer membrane and facilitates pathogen transmission from ticks to mice.borrelia burgdorferi lipoprotein lp6.6 is a differentially produced spirochete antigen. an assessment of lp6.6 expression covering representative stages of the infectious cycle of spirochetes demonstrates that the gene is solely expressed during pathogen persistence in ticks. deletion of lp6.6 in infectious b. burgdorferi did not influence in vitro growth, or its ability to persist and induce inflammation in mice, migrate to larval or nymphal ticks or survive through the larval-nymphal molt. how ...200919703109
borrelia burgdorferi bmpa is a laminin-binding protein.the borrelia burgdorferi bmpa outer surface protein plays a significant role in mammalian infection by the lyme disease spirochete and is an important antigen for the serodiagnosis of human infection. b. burgdorferi adheres to host extracellular matrix components, including laminin. the results of our studies indicate that bmpa and its three paralogous proteins, bmpb, bmpc, and bmpd, all bind to mammalian laminin. bmpa did not bind mammalian type i or type iv collagens or fibronectin. bmpa-direc ...200919703983
possible role of glial cells in the onset and progression of lyme neuroborreliosis.lyme neuroborreliosis (lnb) may present as meningitis, cranial neuropathy, acute radiculoneuropathy or, rarely, as encephalomyelitis. we hypothesized that glia, upon exposure to borrelia burgdorferi, the lyme disease agent, produce inflammatory mediators that promote the acute cellular infiltration of early lnb. this inflammatory context could potentiate glial and neuronal apoptosis.200919706181
phylogeography of borrelia burgdorferi in the eastern united states reflects multiple independent lyme disease emergence events.since its first description in coastal connecticut in 1976, both the incidence of lyme disease and the geographic extent of endemic areas in the us have increased dramatically. the rapid expansion of lyme disease into its current distribution in the eastern half of the us has been due to the range expansion of the tick vector, ixodes scapularis, upon which the causative agent, borrelia burgdorferi is dependent for transmission to humans. in this study, we examined the phylogeographic population ...200919706476
Refresh
firstprev
...71727374757677787980...
nextlast
Displaying items 7801 - 7900 of 8907