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survey of high throughput rna-seq data reveals potential roles for lncrnas during development and stress response in bread wheat.long non-coding rnas (lncrnas) are a family of regulatory rnas that play essential role in the various developmental processes and stress responses. recent advances in sequencing technology and computational methods enabled identification and characterization of lncrnas in certain plant species, but they are less known in triticum aestivum (bread wheat). herein, we analyzed 52 rna seq data (>30 billion reads) and identified 44,698 lncrnas in t. aestivum genome, which were characterized in compar ...201728649263
pattern of protein expression in developing wheat grains identified through proteomic analysis.grain development is one of the biological processes, which contributes to the final grain yield. to understand the molecular changes taking place during the early grain development, we profiled proteomes of two common wheat cultivars p271 and chinese spring (cs) with large and small grains, respectively at three grain developmental stages (4, 8, and 12 days post anthesis). an itraq (isobaric tags for relative and absolute quantitation) based proteomics approach was used for this purpose. more t ...201728649254
soil application of sewage sludge stabilized with steelmaking slag and its effect on soil properties and wheat growth.the effect of sewage sludge, stabilized with steelmaking slag, on soil chemical properties and fertility and on wheat (triticum aestivum l.) growth was evaluated. dewatered sewage sludge [75% (wet weight basis)] stabilized with steelmaking slag (25%) and three soils with different ph values were used in a pot experiment with winter wheat. the following treatments were applied: (i) sludge addition of 30gkg-1 (≈ 120mgha-1, rate equivalent to the common inorganic n fertilization for wheat, based on ...201728647222
isolation of two new retrotransposon sequences and development of molecular and cytological markers for dasypyrum villosum (l.).dasypyrum villosum is a valuable genetic resource for wheat improvement. with the aim to efficiently monitor the d. villosum chromatin introduced into common wheat, two novel retrotransposon sequences were isolated by rapd, and were successfully converted to d. villosum-specific scar markers. in addition, we constructed a chromosomal karyotype of d. villosum. our results revealed that different accessions of d. villosum showed slightly different signal patterns, indicating that distribution of r ...201728638972
in vivo label-free mapping of the effect of a photosystem ii inhibiting herbicide in plants using chlorophyll fluorescence lifetime.in order to better understand and improve the mode of action of agrochemicals, it is useful to be able to visualize their uptake and distribution in vivo, non-invasively and, ideally, in the field. here we explore the potential of plant autofluorescence (specifically chlorophyll fluorescence) to provide a readout of herbicide action across the scales utilising multiphoton-excited fluorescence lifetime imaging, wide-field single-photon excited fluorescence lifetime imaging and single point fluore ...201728638436
wheat seed weight and quality differ temporally in sensitivity to warm or cool conditions during seed development and maturation.short periods of extreme temperature may affect wheat (triticum aestivum) seed weight, but also quality. temporal sensitivity to extreme temperature during seed development and maturation was investigated.201728637252
beneficial effects of melatonin in overcoming drought stress in wheat seedlings.melatonin plays an important role in abiotic stress in plant, but its role in wheat drought tolerance is less known. to verify its role, wheat seedlings (triticum aestivum l. 'yan 995') at 60% and 40% of field capacity were treated with 500 μm melatonin in this study. melatonin treatment significantly enhanced the drought tolerance of wheat seedlings, as demonstrated by decreased membrane damage, more intact grana lamella of chloroplast, higher photosynthetic rate, and maximum efficiency of phot ...201728633086
a genome-wide association study of 23 agronomic traits in chinese wheat landraces.uncovering the genetic basis of agronomic traits in wheat landraces is important for ensuring global food security via the development of improved varieties. here, 723 wheat landraces from 10 chinese agro-ecological zones were evaluated for 23 agronomic traits in six environments. all accessions could be clustered into five subgroups based on phenotypic data via discriminant function analysis, which was highly consistent with genotypic classification. a genome-wide association study was conducte ...201728628238
the auxin biosynthetic tryptophan aminotransferase related tatar2.1-3a increases grain yield of wheat.controlling the major auxin biosynthetic pathway to manipulate auxin content could be a target for genetic engineering of crops with desired traits, but little progress had been made because low or high auxin contents often cause developmental inhibition. here, we performed a genome-wide analysis of bread wheat (triticum aestivum) to identify the tryptophan aminotransferase of arabidopsis1/tryptophan aminotransferase-related (taa1/tar) genes that function in the tryptophan-dependent pathway of a ...201728626005
effect of the fungus piriformospora indica on physiological characteristics and root morphology of wheat under combined drought and mechanical stresses.this study was done to evaluate the effects of the root-colonizing endophytic fungus piriformospora indica on wheat growth under combined drought and mechanical stresses. inoculated (colonized) and non-inoculated (uncolonized) wheat (triticum aestivum l. cv. chamran) seedlings were planted in growth chambers filled with moist sand (at a matric suction of 20 hpa). slight, moderate and severe mechanical stresses (i.e., penetration resistance, qp, of 1.17, 4.17 and 5.96 mpa, respectively) were prod ...201728624682
expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation.mitochondrial respiratory components participate in the maintenance of chloroplast functional activity. this study investigates the effects 48h de-etiolation of spring wheat seedlings (triticum aestivum l., var. irgina) on the expression of genes that encode energy-dissipating respiratory components and antioxidant enzymes under continuous light conditions. the expression of aox1a following the prolonged darkness exhibited a pattern indicating a prominent dependence on light. the expression of o ...201728623839
commentary on "characteristics of cadmium uptake and membrane transport in roots of intact wheat (triticum aestivum l.) seedlings" reported by lian-zhen li, chen tu, willie j.g.m. peijnenburg, yong-ming luo. 201728622963
transcriptome analysis of drought-responsive genes regulated by hydrogen sulfide in wheat (triticum aestivum l.) leaves.drought is an environmental factor that deeply impacts wheat yield and quality. hydrogen sulfide (h2s) is a known regulator of drought resistance in plants. to preliminarily elucidate the regulatory mechanisms of h2s on drought tolerance, the effects of h2s on drought-responsive genes were investigated by transcriptome analysis. as a result, a total of 7552 transcripts not only responded to drought stress but also exhibited differential expression relative to the polyethylene glycol (peg) treatm ...201728620758
genome-wide association study of grain architecture in wild wheat aegilops tauschii.aegilops tauschii, the d-genome progenitor of triticum aestivum, encompasses huge diversity for various traits of potential economic importance such as yield, biotic and abiotic stress tolerance, quality and nutrition. in the present study, variation for grain size in ae. tauschii germplasm was studied and its genetic basis dissected using genome-wide association study (gwas). grain length, width, and weight evaluated in 177 ae. tauschii accessions over 3 years showed near normal distribution wi ...201728620398
preliminary evidence for associations between molecular markers and quantitative traits in a set of bread wheat (triticum aestivum l.) cultivars and breeding lines.the identification of polymorphic markers associated with various quantitative traits allows us to test their performance for the exploitation of the extensive quantitative variation maintained in gene banks. in the current study, a set of 97 wheat germplasm accessions including 48 cultivars and 49 breeding lines were evaluated for 18 agronomic traits. the accessions were also genotyped with 23 issr, nine irap and 20 remap markers, generating a total of 658 clear and scorable bands, 86% of which ...201728619368
silencing of a lipase maturation factor 2-like gene by wheat-mediated rnai reduces the survivability and reproductive capacity of the grain aphid, sitobion avenae.lipase maturation factor (lmf) family proteins are required for the maturation and transport of active lipoprotein lipases. however, the specific roles of lmf2 remain unknown. in this study, a grain aphid lmf2-like gene fragment was cloned and was highly similar in sequence to a homologous gene in the pea aphid, acyrthosiphon pisum. an rnai vector was constructed with this fragment and used for wheat transformation. the expression of the lmf2-like gene in aphid, as well as the growth and reprodu ...201728618004
differential effects of a post-anthesis heat stress on wheat (triticum aestivum l.) grain proteome determined by itraq.heat stress, a major abiotic stressor of wheat (triticum aestivum l.), often results in reduced yield and decreased quality. in this study, a proteomic method, tags for relative and absolute quantitation isobaric (itraq), was adopted to analyze the protein expression profile changes among wheat cultivar jing411 under heat stress. results indicated that there were 256 different proteins expressed in jing411 under heat stress. according to the result of gene annotation and functional classificatio ...201728615669
effects of cultivar and nitrogen nutrition on the lipid composition of wheat flour.despite being minor components of flour, wheat (triticum aestivum l.) lipids contribute to grain processing. they are particularly important for bread making, where they adsorb to the surface of gas bubbles formed during the proving stage of bread making, stabilizing the gas cells and improving gas retention within the dough. this contributes to the volume and texture of the loaf. however, little is understood about how their amount, composition, and properties vary in response to genotype (g), ...201728614658
molecular cloning and characterizations of xylanase inhibitor protein from wheat (triticum aestivum).xylanase inhibitor proteins (xips) were regarded to inhibit the activity of xylanases during baking and gluten-starch separation processes. to avoid the inhibition to xylanases, it is necessary to define the conditions under which the inhibition takes place. in this study, we cloned the xip gene from 2 different variety of triticum aestivum, that is, zhengmai 9023 and zhengmai 366, and investigated the properties of xip protein expressed by pichia pastoris. the results showed that the 2 xip gene ...201728613409
oligonucleotides and nd-fish displaying different arrangements of tandem repeats and identification of dasypyrum villosum chromosomes in wheat backgrounds.oligonucleotide probes and the non-denaturing fluorescence in situ hybridization (nd-fish) technique are widely used to analyze plant chromosomes because they are convenient tools. new oligonucleotide probes, oligo-ku, oligo-3b117.1, oligo-3b117.2, oligo-3b117.2.1, oligo-3b117.3, oligo-3b117.4, oligo-3b117.5, oligo-3b117.6, oligo-pta71a-1, oligo-pta71a-2, oligo-pta71b-1, oligo-pta71b-2, oligo-pta71c-1, oligo-pta71c-2, oligo-pta71c-3 and oligo-pta71d were designed based on the repetitive sequence ...201728613230
evaluation of antiproliferative and hepatoprotective effects of wheat grass (triticum aestivum).this study was aimed to evaluate the pharmacological potential of various extracts (hexane, chloroform, methanol and aqueous) of dried shoots of triticum aestivum (wheat grass) in terms of antiproliferative and hepatoprotective potential of t. aestivum. the total chlorophyll content in dried shoots of t. aestivum was 0.54 ± 0.016 g/l (chlorophyll-a: 0.288 ± 0.05 g/l; and chlorophyll-b; 0.305 ± 0.05 g/l), while total carotene content was 0.42 ± 0.066 g/l. in addition, the chloroform extract of dr ...201728605975
evolution of the mir5200-flowering locus t flowering time regulon in the temperate grass subfamily pooideae.flowering time is a carefully regulated trait controlled primarily through the action of the central genetic regulator, flowering locus t (ft). recently it was demonstrated that a microrna, mir5200, targets the end of the second exon of ft under short-day photoperiods in the grass subfamily pooideae, thus preventing ft transcripts from reaching threshold levels under non-inductive conditions. pooideae are an interesting group in that they rapidly diversified from the tropics into the northern te ...201728603035
inclusion of wheat and triticale silage in the diet of lactating dairy cows.the objective of this experiment was to partially replace corn silage with 2 alternative forages, wheat (triticum aestivum) or triticale (x triticosecale) silages at 10% of the diet dry matter (dm), and investigate the effects on dairy cow productivity, nutrient utilization, enteric ch4 emissions, and farm income over feed costs. wheat and triticale were planted in the fall as cover crops and harvested in the spring at the boot stage. neutral- and acid-detergent fiber and lignin concentrations w ...201728601456
escitalopram or novel herbal treatments differentially alter cytokine and behavioral responses to immune challenge.studies suggest that inflammation is involved in the pathophysiology of depression. the present study examined the effects of the commonly used antidepressant escitalopram, in comparison with a novel herbal treatment (nht) consisted of crataegus pinnatifida, triticum aestivum, lilium brownii and fructus ziziphus jujuba, on cytokine and behavioral responses to an immune challenge. escitalopram augmented lipopolysaccharide-induced tumor necrosis factor (tnf)-α peripheral secretion and induced a fa ...201728601279
root tip shape governs root elongation rate under increased soil strength.increased soil strength due to soil compaction or soil drying is a major limitation to root growth and crop productivity. roots need to exert higher penetration force, resulting in increased penetration stress when elongating in soils of greater strength. this study aimed to quantify how the genotypic diversity of root tip geometry and root diameter influences root elongation under different levels of soil strength and to determine the extent to which roots adjust to increased soil strength. fou ...201728600344
characterization of aldehyde oxidase (ao) genes involved in the accumulation of carotenoid pigments in wheat grain.aldehyde oxidase (ao) enzyme (ec 1.2.3.1) catalyzes the final steps of carotenoid catabolism and it is a key enzyme in the abscisic acid (aba) biosynthesis. ao isoforms are located in the cytosolic compartment of tissues in many plants, where induce the oxidation of aldehydes into carboxylic acid, and in addition, catalyze the hydroxylation of some heterocycles. the goal of the present study was to characterize the ao genes involved in the accumulation of carotenoid pigments in wheat grain, an i ...201728596779
host plants of the wheat stem sawfly (hymenoptera: cephidae).wheat stem sawfly (cephus cinctus norton) is a pest of economic importance across much of the wheat (triticum aestivum l.)-growing areas of the western great plains of north america as well as an ecologically important insect owing to its wide range of grass hosts. little research has been published regarding the noncultivated native and invasive grasses attacked by this insect. knowledge of the complete host range of c. cinctus can inform future research about potential new sources of genetic r ...201728591774
quantitative trait loci for slow-rusting resistance to leaf rust in doubled-haploid wheat population ci13227 × lakin.ci13227 is a u.s. winter wheat line with adult-plant slow-rusting resistance that has been the subject of several studies on the characteristics and components of slow rusting. previous genetic studies used different populations and approaches and came to different conclusions about the genetic basis of resistance in ci13227. to clarify the situation, a new doubled-haploid (dh) population of ci13227 × lakin was produced and a linkage map was constructed using 5,570 single-nucleotide polymorphism ...201728589757
changes in low-molecular-weight thiol-disulphide redox couples are part of bread wheat seed germination and early seedling growth.the tripeptide antioxidant glutathione (γ-l-glutamyl-l-cysteinyl-glycine; gsh) essentially contributes to thiol-disulphide conversions, which are involved in the control of seed development, germination, and seedling establishment. however, the relative contribution of gsh metabolism in different seed structures is not fully understood. we studied the gsh/glutathione disulphide (gssg) redox couple and associated low-molecular-weight (lmw) thiols and disulphides related to gsh metabolism in bread ...201728580817
the proportion of nitrate in leaf nitrogen, but not changes in root growth, are associated with decreased grain protein in wheat under elevated [co2].the atmospheric co2 concentration ([co2]) is increasing and predicted to reach ∼550ppm by 2050. increasing [co2] typically stimulates crop growth and yield, but decreases concentrations of nutrients, such as nitrogen ([n]), and therefore protein, in plant tissues and grains. such changes in grain composition are expected to have negative implications for the nutritional and economic value of grains. this study addresses two mechanisms potentially accountable for the phenomenon of elevated [co2]- ...201728575746
the contrasting microrna content of a drought tolerant and a drought susceptible wheat cultivar.drought stress represents one of the most common stresses affecting the productivity of crop plants. a rather recently discovered component of the plant response to drought is the cellular population of micrornas. here, the microrna content was revealed of two bread wheat cultivars contrasting strongly with respect to the ability to withstand drought stress. a total of 1813 mirnas was identified, grouped into 106 families. some 104 of these mirnas were predicted to match 212 novel mirna precurso ...201728575745
impact of surface charge on cerium oxide nanoparticle uptake and translocation by wheat (triticum aestivum).nanoparticle (np) physiochemical properties, including surface charge, affect cellular uptake, translocation, and tissue localization. to evaluate the influence of surface charge on np uptake by plants, wheat seedlings were hydroponically exposed to 20 mg/l of ∼4 nm ceo2 nps functionalized with positively charged, negatively charged, and neutral dextran coatings. fresh, hydrated roots and leaves were analyzed at various time points over 34 h using fluorescence x-ray absorption near-edge spectros ...201728575574
exploring the heat-responsive chaperones and microsatellite markers associated with terminal heat stress tolerance in developing wheat.global warming is a major threat for agriculture and food security, and in many cases the negative impacts are already apparent. wheat is one of the most important staple food crops and is highly sensitive to the heat stress (hs) during reproductive and grain-filling stages. here, whole transcriptome analysis of thermotolerant wheat cv. hd2985 was carried out at the post-anthesis stage under control (22 ± 3 °c) and hs-treated (42 °c, 2 h) conditions using illumina hiseq and roche gs-flx 454 plat ...201728573536
massive expansion and differential evolution of small heat shock proteins with wheat (triticum aestivum l.) polyploidization.wheat (triticum aestivum), one of the world's most important crops, is facing unprecedented challenges due to global warming. to evaluate the gene resources for heat adaptation in hexaploid wheat, small heat shock proteins (shsps), the key plant heat protection genes, were comprehensively analysed in wheat and related species. we found that the shsps of hexaploid wheat were massively expanded in a and b subgenomes with intrachromosomal duplications during polyploidization. these expanded shsps w ...201728566710
a systematic review of rye (secale cereale l.) as a source of resistance to pathogens and pests in wheat (triticum aestivum l.).wheat is globally one of the most important crops. with the current human population growth rate, there is an increasing need to raise wheat productivity by means of plant breeding, along with development of more efficient and sustainable agricultural systems. damage by pathogens and pests, in combination with adverse climate effects, need to be counteracted by incorporating new germplasm that makes wheat more resistant/tolerant to such stress factors. rye has been used as a source for improved ...201728559761
global identification, structural analysis and expression characterization of bhlh transcription factors in wheat.basic helix-loop-helix (bhlh) transcription factors (tfs), which are widely distributed in eukaryotic organisms, play crucial roles in plant development. however, no comprehensive analysis of the bhlh family in wheat (triticum aestivum l.) has been undertaken previously.201728558686
potassium and zinc increase tolerance to salt stress in wheat (triticum aestivum l.).potassium and zinc are essential elements in plant growth and metabolism and plays a vital role in salt stress tolerance. to investigate the physiological mechanism of salt stress tolerance, a pot experiment was conducted. potassium and zinc significantly minimize the oxidative stress and increase root, shoot and spike length in wheat varieties. fresh and dry biomass were significantly increased by potassium followed by zinc as compared to control c. the photosynthetic pigment and osmolyte regul ...201728558283
effects of tio2 nanoparticles on wheat (triticum aestivum l.) seedlings cultivated under super-elevated and normal co2 conditions.concerns over the potential risks of nanomaterials to ecosystem have been raised, as it is highly possible that nanomaterials could be released to the environment and result in adverse effects on living organisms. carbon dioxide (co2) is one of the main greenhouse gases. the level of co2 keeps increasing and subsequently causes a series of environmental problems, especially for agricultural crops. in the present study, we investigated the effects of tio2 nps on wheat seedlings cultivated under s ...201728558015
stem carbohydrate dynamics and expression of genes involved in fructan accumulation and remobilization during grain growth in wheat (triticum aestivum l.) genotypes with contrasting tolerance to water stress.the genetic and physiological mechanisms underlying the relationship between water-soluble carbohydrates (wsc) and water stress tolerance are scarcely known. this study aimed to evaluate the main wsc in stems, and the expression of genes involved in fructan metabolism in wheat genotypes growing in a glasshouse with water stress (ws; 50% field capacity from heading) and full irrigation (fi; 100% field capacity). eight wheat genotypes (five tolerant and three susceptible to water stress) were eval ...201728552955
low-dose ionizing radiation limitations to seed germination: results from a model linking physiological characteristics and developmental-dynamics simulation strategy.there is much uncertainty about the risks of seed germination after repeated or protracted environmental low-dose ionizing radiation exposure. the purpose of this study is to explore the influence mechanism of low-dose ionizing radiation on wheat seed germination using a model linking physiological characteristics and developmental-dynamics simulation. a low-dose ionizing radiation environment simulator was built to investigate wheat (triticum aestivum l.) seeds germination process and then a ki ...201728551365
high-throughput snp genotyping of modern and wild emmer wheat for yield and root morphology using a combined association and linkage analysis.durum wheat (triticum turgidum var. durum desf.) is a major world crop that is grown primarily in areas of the world that experience periodic drought, and therefore, breeding climate-resilient durum wheat is a priority. high-throughput single nucleotide polymorphism (snp) genotyping techniques have greatly increased the power of linkage and association mapping analyses for bread wheat, but as yet there is no durum wheat-specific platform available. in this study, we evaluate the new 384ht wheat ...201728550605
short periods of high temperature during meiosis prevent normal meiotic progression and reduce grain number in hexaploid wheat (triticum aestivum l.).exposure of wheat to high temperatures during male meiosis prevents normal meiotic progression and reduces grain number. we define a temperature-sensitive period and link heat tolerance to chromosome 5d. this study assesses the effects of heat on meiotic progression and grain number in hexaploid wheat (triticum aestivum l. var. chinese spring), defines a heat-sensitive stage and evaluates the role of chromosome 5d in heat tolerance. plants were exposed to high temperatures (30 or 35 °c) in a con ...201728550436
quantifying wheat sensitivities to environmental constraints to dissect genotype × environment interactions in the field.yield is subject to strong genotype-by-environment (g × e) interactions in the field, especially under abiotic constraints such as soil water deficit (drought [d]) and high temperature (heat [h]). since environmental conditions show strong fluctuations during the whole crop cycle, geneticists usually do not consider environmental measures as quantitative variables but rather as factors in multienvironment analyses. based on 11 experiments in a field platform with contrasting temperature and soil ...201728546436
wheat root length and not branching is altered in the presence of neighbours, including blackgrass.the effect of neighbouring plants on crop root system architecture may directly interfere with water and nutrient acquisition, yet this important and interesting aspect of competition remains poorly understood. here, the effect of the weed blackgrass (alopecurus myosuroides huds.) on wheat (triticum aestivum l.) roots was tested, since a low density of this species (25 plants m-2) can lead to a 10% decrease in wheat yield and herbicide resistance is problematic. we used a simplified growth syste ...201728542446
developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance.the main aim of this study was to improve fungal resistance in bread wheat via transgenesis. transgenic wheat plants harboring barley chitinase (chi26) gene, driven by maize ubi promoter, were obtained using biolistic bombardment, whereas the herbicide resistance gene, bar, driven by the camv 35s promoter was used as a selectable marker.201728539970
ancestral qtl alleles from wild emmer wheat enhance root development under drought in modern wheat.a near-isogenic line (nil-7a-b-2), introgressed with a quantitative trait locus (qtl) on chromosome 7as from wild emmer wheat (triticum turgidum ssp. dicoccoides) into the background of bread wheat (t. aestivum l.) cv. barnir, was recently developed and studied in our lab. nil-7a-b-2 exhibited better productivity and photosynthetic capacity than its recurrent parent across a range of environments. here we tested the hypothesis that root-system modifications play a major role in nil-7a-b-2's agro ...201728536586
high-resolution mapping of crossover events in the hexaploid wheat genome suggests a universal recombination mechanism.during meiosis, crossovers (cos) create new allele associations by reciprocal exchange of dna. in bread wheat (triticum aestivum l.), cos are mostly limited to subtelomeric regions of chromosomes, resulting in a substantial loss of breeding efficiency in the proximal regions, though these regions carry ∼60-70% of the genes. identifying sequence and/or chromosome features affecting recombination occurrence is thus relevant to improve and drive recombination. using the recent release of a referenc ...201728533438
pre-drought priming sustains grain development under post-anthesis drought stress by regulating the growth hormones in winter wheat (triticum aestivum l.).drought stress during grain filling is the most yield-damaging to wheat. pre-drought priming facilitated the wheat plants to sustain grain development against the post-anthesis drought stress by modulating the levels of growth hormones. post-anthesis drought stress substantially reduces grain yield in wheat (triticum aestivum l.) due to impaired grain development associated with imbalanced levels of growth hormones. to investigate whether pre-drought priming could sustain grain development in wh ...201728526982
mapping qtls controlling kernel dimensions in a wheat inter-varietal ril mapping population.seven kernel dimension qtls were identified in wheat, and kernel thickness was found to be the most important dimension for grain weight improvement. kernel morphology and weight of wheat (triticum aestivum l.) affect both yield and quality; however, the genetic basis of these traits and their interactions has not been fully understood. in this study, to investigate the genetic factors affecting kernel morphology and the association of kernel morphology traits with kernel weight, kernel length ( ...201728526913
mir156-targeted sbp-box transcription factors interact with dwarf53 to regulate teosinte branched1 and barren stalk1 expression in bread wheat.genetic and environmental factors affect bread wheat (triticum aestivum) plant architecture, which determines grain yield. in this study, we demonstrate that mir156 controls bread wheat plant architecture. we show that overexpression of tae-mir156 in bread wheat cultivar kenong199 leads to increased tiller number and severe defects in spikelet formation, probably due to the tae-mir156-mediated repression of a group of squamosa promoter binding protein-like (spl) genes. furthermore, we found that ...201728526703
the effect of sewage sludge on heavy metal concentrations in wheat plant (triticum aestivum l.).the use of sewage sludge in agricultural soils can improve soil physical and chemical properties and soil fertility by increasing organic materials; however, the presence of heavy metals in sewage sludge is a significant problem for the quality of agricultural products and the environment. most heavy metals, due to their inactive nature, are stable and can affect human health. this study investigates the effect of sewage sludge on the concentration of copper, zinc, cadmium, lead, iron, and manga ...201728523618
a novel, economical way to assess virulence in field populations of hessian fly (diptera: cecidomyiidae) utilizing wheat resistance gene h13 as a model.mayetiola destructor (say) is a serious pest of wheat, triticum aestivum l., in north america, north africa, and central asia. singly deployed resistance genes in wheat cultivars have provided effective management of hessian fly populations for >50 yr. thirty-five h genes have been documented. defense mediated by the h gene constitutes strong selection on the hessian fly population, killing 100% of larvae. a mutation in a matching hessian fly avirulence gene confers virulence to the h gene, lead ...201728520950
ms26/cyp704b is required for anther and pollen wall development in bread wheat (triticum aestivum l.) and combining mutations in all three homeologs causes male sterility.development of anthers and pollen represents an important aspect of the life cycle in flowering plants. genes contributing to anther and pollen development have been widely studied in many plant species. ms26/cyp704b genes play an important role in pollen development through biosynthesis of sporopollenin for pollen exine formation. to investigate the role of ms26/cyp704b genes in anther and pollen development of bread wheat, mutations in the a-, b-, and d-homeologs of the putative ms26/cyp704b g ...201728520767
genetics of mycorrhizal symbiosis in winter wheat (triticum aestivum).bread wheat (triticum aestivum) is a major staple food and therefore of prime importance for feeding the earth's growing population. mycorrhiza is known to improve plant growth, but although extensive knowledge concerning the interaction between mycorrhizal fungi and plants is available, genotypic differences concerning the ability of wheat to form mycorrhizal symbiosis and quantitative trait loci (qtls) involved in mycorrhization are largely unknown. therefore, a diverse set of 94 bread wheat g ...201728517039
gene introgression from common wheat into aegilops l.group of experiments were carried out to verify possibility of gene introgression from common wheat into aegilops. the artificial indoor crossbreed was conducted using 7 genotypes from 4 wheat relative species as female, and common wheat as male. the experiment result shows that different species has variable cross ability. among the 4 aegilops species, the highest cross rate is from the combination of aegilops tauschii × triticum aestivum (46.49% for genotype ae42, 22.58% for y92), the second i ...201728490951
mapk-mediated auxin signal transduction pathways regulate the malic acid secretion under aluminum stress in wheat (triticum aestivum l.).an isobaric tags for relative and absolute quantitative (itraq)-based quantitative proteomic approach was used to screen the differentially expressed proteins during control treatment (ck), aluminum (al) and al+ indole-3-acetic acid (iaa) treatment of wheat lines et8 (al-tolerant). further, the the expression levels of auxin response factor (arf), aux/iaa, mitogen activated protein kinase (mapk) 2c, and mapk1a were analyzed. results showed that 16 proteins were determined to be differentially ex ...201728487539
identification and positional distribution analysis of transcription factor binding sites for genes from the wheat fl-cdna sequences.the binding sites of transcription factors (tfs) in upstream dna regions are called transcription factor binding sites (tfbss). tfbss are important elements for regulating gene expression. to date, there have been few studies on the profiles of tfbss in plants. in total, 4,873 sequences with 5' upstream regions from 8530 wheat fl-cdna sequences were used to predict tfbss. we found 4572 tfbss for the mads tf family, which was twice as many as for bhlh (1951), b3 (1951), hb superfamily (1914), erf ...201728485207
genome-wide analysis of wrky transcription factors in wheat (triticum aestivum l.) and differential expression under water deficit condition.wrky proteins, which comprise one of the largest transcription factor (tf) families in the plant kingdom, play crucial roles in plant development and stress responses. despite several studies on wrkys in wheat (triticum aestivum l.), functional annotation information about wheat wrkys is limited.201728484671
qtl mapping for phosphorus efficiency and morphological traits at seedling and maturity stages in wheat.phosphorus (p) efficiency (pe), which comprises phosphorus uptake (pupe) and utilization efficiency (pute), is considered as one of the most important factors for crop yield. in the present study, 11 seedling traits and 13 maturity traits related to wheat pe and morphology were investigated using a set of recombinant inbred lines (rils) derived from the cross of "tn 18 × lm 6," under hydroponic culture trials and field trials at low p (lp) and normal p (np) levels in two different years, respect ...201728484481
the impact of drought on wheat leaf cuticle properties.the plant cuticle is the outermost layer covering aerial tissues and is composed of cutin and waxes. the cuticle plays an important role in protection from environmental stresses and glaucousness, the bluish-white colouration of plant surfaces associated with cuticular waxes, has been suggested as a contributing factor in crop drought tolerance. however, the cuticle structure and composition is complex and it is not clear which aspects are important in determining a role in drought tolerance. th ...201728482800
differential representation of albumins and globulins during grain development in durum wheat and its possible functional consequences.durum wheat (triticum turgidum ssp. durum (desf.) husn.) is an economically important crop used for the production of semolina, which is the basis of pasta and other food products. its grains provide proteins and starch for human consumption. grain development is a key process in wheat physiology; it is highly affected by a number of enzymes that control the metabolic processes governing accumulation of starch and storage proteins and ultimately grain weight. most of these enzymes are present in ...201728478307
characterisation of the nicotianamine aminotransferase and deoxymugineic acid synthase genes essential to strategy ii iron uptake in bread wheat (triticum aestivum l.).iron (fe) uptake in graminaceous plant species occurs via the release and uptake of fe-chelating compounds known as mugineic acid family phytosiderophores (mas). in the mas biosynthetic pathway, nicotianamine aminotransferase (naat) and deoxymugineic acid synthase (dmas) enzymes catalyse the formation of 2'-deoxymugineic acid (dma) from nicotianamine (na). here we describe the identification and characterisation of six tanaat and three tadmas1 genes in bread wheat (triticum aestivum l.). the cod ...201728475636
novel insights into the effect of nitrogen on storage protein biosynthesis and protein body development in wheat caryopsis.molecular and cytological mechanisms concerning the effects of nitrogen on wheat (triticum aestivum l.) storage protein biosynthesis and protein body development remain largely elusive. we used transcriptome sequencing, proteomics techniques, and light microscopy to investigate these issues. in total, 2585 differentially expressed genes (degs) and 57 differentially expressed proteins (deps) were found 7 days after anthesis (daa), and 2456 degs and 64 deps were detected 18 daa after nitrogen trea ...201728472326
using simulation models to investigate the cumulative effects of sowing rate, sowing date and cultivar choice on weed competition.with the increasing pressure on crop production from the evolution of herbicide resistance, farmers are increasingly adopting integrated weed management (iwm) strategies to augment their weed control. these include measures to increase the competitiveness of the crop canopy such as increased sowing rate and the use of more competitive cultivars. while there are data on the relative impact of these non-chemical weed control methods assessed in isolation, there is uncertainty about their combined ...201728469287
agricultural factors affecting fusarium communities in wheat kernels.fusarium head blight (fhb) is a devastating disease of cereals caused by fusarium fungi. the disease is of great economic importance especially owing to reduced grain quality due to contamination by a range of mycotoxins produced by fusarium. disease control and prediction is difficult because of the many fusarium species associated with fhb. different species may respond differently to control methods and can have both competitive and synergistic interactions. therefore, it is important to unde ...201728463719
dynamic nucleolar activity in wheat × aegilops hybrids: evidence of c-genome dominance.nor loci of c-subgenome are dominant in wheat × aegilops interspecific hybrids, which may have evolutionary implications for wheat group genome dynamics and evolution. after interspecific hybridisation, some genes are often expressed from only one of the progenitor species, shaping subsequent allopolyploid genome evolution processes. a well-known example is nucleolar dominance, i.e. the formation of cell nucleoli from chromosomes of only one parental species. we studied nucleolar organizing regi ...201728456843
wheat ms2 encodes for an orphan protein that confers male sterility in grass species.male sterility is a valuable trait for plant breeding and hybrid seed production. the dominant male-sterile gene ms2 in common wheat has facilitated the release of hundreds of breeding lines and cultivars in china. here, we describe the map-based cloning of the ms2 gene and show that ms2 confers male sterility in wheat, barley and brachypodium. ms2 appears as an orphan gene within the triticinae and expression of ms2 in anthers is associated with insertion of a retroelement into the promoter. th ...201728452349
wheatnet: a genome-scale functional network for hexaploid bread wheat, triticum aestivum. 201728450181
three dominant awnless genes in common wheat: fine mapping, interaction and contribution to diversity in awn shape and length.the awn is a long needle-like structure formed at the tip of the lemma in the florets of some grass species. it plays a role in seed dispersal and protection against animals, and can contribute to the photosynthetic activity of spikes. three main dominant inhibitors of awn development (hd, b1 and b2) are known in hexaploid wheat, but the causal genes have not been cloned yet and a genetic association with awn length diversity has been found only for the b1 allele. to analyze the prevalence of th ...201728437453
localization of benzoxazinones that occur constitutively in wheat seedlings.occurrence and localization of novel antimicrobial and antifeeding compounds in wheat, 2,4-dihydroxy-1,4-benzoxazin-3-one (diboa) and 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (dimboa), and their glucosides, were examined by staining wheat plants (triticum aestivum l.) in the juvenile stage of growth by ferric chloride. the methanol extracts of the stained plant tissues gave a characteristic blue color, which was shown by spectroscopic and chromatographic analyses to be exclusively due to ben ...20179031524
puroindolines: the molecular genetic basis of wheat grain hardness.the variation in grain hardness is the single most important trait that determines end-use quality of wheat. grain texture classification is based primarily on either the resistance of kernels to crushing or the particle size distribution of ground grain or flour. recently, the molecular genetic basis of grain hardness has become known, and it is the focus of this review. the puroindoline proteins a and b form the molecular basis of wheat grain hardness or texture. when both puroindolines are in ...201711999840
detection of grain protein content qtls across environments in tetraploid wheats.grain protein content (gpc) is an important quality factor in both durum and bread wheats. gpc is considered to be a polygenic trait influenced by environmental factors and management practice. the objectives of this study were both to compare the quantitative trait loci (qtl) for gpc in a population of 65 recombinant inbred lines of tetraploid wheats evaluated in three locations for several years (eight data sets), and to investigate the genetic relationship among gpc and grain yield. qtls were ...201711999838
poultry litter ash as a potential phosphorus source for agricultural crops.maryland will impose restrictions on poultry litter application to soils with excessive p by the year 2005. alternative uses for poultry litter are being considered, including burning as a fuel to generate electricity. the resulting ash contains high levels of total p, but the availability for crop uptake has not been reported. our objective was to compare the effectiveness of poultry litter ash (pla) and potassium phosphate (kp) as a p source for wheat (triticum aestivum l.) in acidic soils, wi ...201712026100
[protective role of kartolin-4 in soil drought in wheat plants].parameters of photosynthesis, heat-resistance, and osmotic pressure of cell exudate of leaves of the drought-sensitive cultivar lyutestsens 758 of wheat, triticum aestivum, were studied under conditions of normal water supply, soil drought, and subsequent rehydration. the plants preliminarily treated with kartolin-4 were compared to untreated plants. kartolin-4, a preparation with cytokininlike activity, partially prevented the drought-induced inhibition of the photosynthetic assimilation of car ...201712325301
dynamics of the cytokinin content in different regions of the ovary in triticum aestivum l. and taraxacum officinale web. during the preparation of the ovicell for division. 201711813552
[generation of negative air ions by wheat seedlings in a high voltage electrization of soil].it was shown that plantlets of wheat (triticum vulgare) are capable of generating negative aeroions during the electrization of soil by high-voltage impulses. soil electrization was carried out either from the moment of planting of seeds or from the appearance of the first seedlings. the concentration of negative ions was measured in the air at a distance of 50 cm from plants. in both variants, similar growth-related changes in the concentration of negative ions were observed. the generation of ...201711855283
utility of leaf senescence-associated gene homologs as developmental markers in common wheat.senescence is the final stage of development in plant tissues. in the senescence process, many senescence-associated genes (sags) are reportedly transcriptionally up-regulated. here, we reported the isolation of nine wheat sag cdna clones named tasag1 to tasag9, and evaluated the usefulness of the sag homologs for wheat developmental molecular markers based on their expression patterns. the nine wheat sags were identified in wheat est libraries based on their homology to rice sags. all wheat sag ...201720850333
[isolation and characterization of an abc-transporter cdna clone from wheat (triticum aestivum l.)].the outmost layer of the surface of any land plant is covered with wax, which protects the inner tissues of plants. the arabidopsis atp binding cassette (abc) transporter, cer5, was found to be an exporter of wax molecules. full-length wheat tacer5 cdna, a sequence homologous to cer5, was isolated. similar to other transporters of the abcg subfamily, tacer5 also has a typical basic structure of nbd-msd (nucleotide-binding folds domain - membrane-spanning domain). although the identity scores of ...201720088377
wheat and sorghum residue removal for expanded uses increases sediment and nutrient loss in runoff.crop residue removal for expanded uses such as feedstocks for cellulosic ethanol production may increase loss of sediment and nutrients in runoff. we assessed on-farm impacts of variable rates of residue removal from no-till winter wheat (triticum aestivum l.) and plow till grain sorghum [sorghum bicolor (l.) moench] on sediment, soil organic carbon (soc) and nutrient losses in runoff in western kansas. five treatments with three replications consisting of removing residues at 0, 25, 50, 75, and ...201719875792
stable sulfur isotope ratio indicates long-term changes in sulfur deposition in the broadbalk experiment since 1845.archived wheat (triticum aestivum l.) grain and straw, and soil samples from the control plot of the rothamsted broadbalk experiment, located in southeastern england and established in 1843, were used to investigate the effects of dramatically changing so2 pollution inputs on the concentrations and stable isotope ratios (delta34s) of s in the samples. representative coal samples from uk major coal fields were also determined for delta34s. concentrations of s showed no clear trends in either grai ...201712549539
hormonal regulation of the onset of endosperm development in amphimictic triticum aestivum l. and apomictic taraxacum officinale web. species. 201712959073
predicting cadmium concentrations in wheat and barley grain using soil properties.the entry of cd into the food chain is of concern as it can cause chronic health problems. to investigate the relationship between soil properties and the concentration of cd in wheat (triticum aestivum l.) and harley (hordeum vulgare l.) grain, we analyzed 162 wheat and 215 barley grain samples collected from paired soil and crop surveys in britain, and wheat and barley samples from two long-term sewage sludge experiments. cadmium concentrations were much lower in barley grain than in wheat gra ...201715074804
1h magnetization transfer in hydrated gluten and flour: effects of wheat aging.the interaction of water with flour or gluten in hydrated samples was investigated by proton magnetization transfer measurements. flour and gluten from both durum and bread wheat seeds, either unaged or artificially aged over different periods of time, were investigated. measurements were performed at several radio frequency power levels and frequency offsets, and the data were quantitatively modeled by two interacting pools, a liquid (water) and a solid (macromolecules) one. a super-lorentzian ...201715360294
preferential flow of bromide, bentazon, and imidacloprid in a dutch clay soil.leaching to ground water and tile drains are important parts of the environmental assessment of pesticides. the aims of the present study were to (i) assess the significance of preferential flow for pesticide leaching under realistic worst-case conditions for dutch agriculture (soil profile with thick clay layer and high rainfall) and (ii) collect a high-quality data set that is suitable for testing pesticide leaching models. the movement of water, bromide, and the pesticides bentazon [3-isoprop ...201715254130
soil properties affecting wheat yields following drilling-fluid application.oil and gas drilling operations use drilling fluids (mud) to lubricate the drill bit and stem, transport formation cuttings to the surface, and seal off porous geologic formations. following completion of the well, waste drilling fluid is often applied to cropland. we studied potential changes in soil compaction as indicated by cone penetration resistance, ph, electrical conductivity (ec(e)), sodium adsorption ratio (sar), extractable soil and total straw and grain trace metal and nutrient conce ...201716091622
long-term cropping system effects on carbon sequestration in eastern oregon.soil organic carbon (soc) has beneficial effects on soil quality and productivity. cropping systems that maintain and/or improve levels of soc may lead to sustainable crop production. this study evaluated the effects of long-term cropping systems on c sequestration. soil samples were taken at 0- to 10-, 10- to 20-, 20- to 30-, and 30- to 40-cm soil depth profiles from grass pasture (gp), conventional tillage (ct) winter wheat (triticum aestivum l.)-fallow (ctwf), and fertilized and unfertilized ...201716825475
disturbance of chlorophyll formation at the level of 5-aminolevulinic acid and mg-containing porphyrin synthesis in isogenic lines of spring wheat (triticum aestivum l.) marked with genes cn-a1 and cn-d1. 201716485648
lecrk-v, an l-type lectin receptor kinase in haynaldia villosa, plays positive role in resistance to wheat powdery mildew.plant sense potential microbial pathogen using pattern recognition receptors (prrs) to recognize pathogen-associated molecular patterns (pamps). the lectin receptor-like kinase genes (lecrks) are involved in various cellular processes mediated by signal transduction pathways. in the present study, an l-type lectin receptor kinase gene lecrk-v was cloned from haynaldia villosa, a diploid wheat relative which is highly resistant to powdery mildew. the expression of lecrk-v was rapidly up-regulated ...201828436098
the stenofolia gene from medicago alters leaf width, flowering time and chlorophyll content in transgenic wheat.molecular genetic analyses revealed that the wuschel-related homeobox (wox) gene superfamily regulates several programs in plant development. many different mechanisms are reported to underlie these alterations. the wox family member stenofolia (stf) is involved in leaf expansion in the eudicot medicago truncutula. here, we report that when this gene was ectopically expressed in a locally adapted hard red winter wheat cultivar (triticum aestivum), the transgenic plants showed not only widened le ...201828509374
pfca uptake and translocation in dominant wheat species (triticum aestivum l.).the comparative uptake of four perfluorinated carboxylic acids (pfcas) by wheat (triticum aestivum l.) grown in nutrient solution was investigated. wheat is the main food crop in northern china and may become a potential pathway of human exposure to these pfcas. the uptake of four pfcas from water at a fixed concentration (1 μg/ml) increased over time, approaching a steady state, and except for the short-chain perfluorobutanoic acid, most of the total mass of each of the pfcas taken up by wheat ...201828598222
effects of free air carbon dioxide enrichment (face) on nitrogen assimilation and growth of winter wheat under nitrate and ammonium fertilization.a 2-year free air co2 enrichment (face) experiment was conducted with winter wheat. it was investigated whether elevated atmospheric co2 concentration (e[co2 ]) inhibit nitrate assimilation and whether better growth and nitrogen acquisition under e[co2 ] can be achieved with an ammonium-based fertilization as it was observed in hydroponic culture with wheat. under e[co2 ] a decrease in nitrate assimilation has been discussed as the cause for observed declines in protein concentration in c3 cerea ...201828715112
differential expression of albumins and globulins of wheat flours of different technological qualities revealed by nanouplc-udmse.soluble proteins were extracted from common wheat flour obtained from nine cultivars of different qualities and were analyzed by nanouplc and ultra definition mass spectrometry (udmse) label-free quantitative approach. collectively, 5894 proteins were identified and quantified with 8 peptides/protein. a total of 414 proteins were found differentially expressed with 85% proteins not yet described in the literature, according to their biological function. quality-related proteins, such as puroindo ...201828873519
bioactive compounds and antioxidant properties of pseudocereals-enriched water biscuits and their in vitro digestates.carotenoids, tocols, phenolic acids and antioxidant capacity were studied during in vitro digestion of water biscuits (wb) from bread wheat, einkorn and einkorn-pseudocereals. antioxidant and cytotoxic activities of digestates were also measured using a 70% caco2/30% ht-29 human intestinal co-culture layer. antioxidant profiles differed among wb formulations. only hydrophilic molecules were solubilised by gastric digestion. after intestinal digestion, 77% carotenoids and 67% tocols were released ...201828946344
a novel approach based on untargeted lipidomics reveals differences in the lipid pattern among durum and common wheat.in the present work the possibility of using an untargeted metabolomic strategy to discriminate between common and durum wheat lipidome for an authenticity purpose was explored. a first study was conducted by analyzing 52 samples from two durum and common wheat varieties. afterwards, an extended and independent sample set (173 samples and five varieties) was used as a confirmatory study to verify the stability and consistency of the models obtained. putatively identified markers were evaluated a ...201828946342
free and esterified carotenoids in pigmented wheat, tritordeum and barley grains.carotenoids are important phytonutrients responsible for the yellow endosperm color in cereal grains. five carotenoids, namely lutein, zeaxanthin, antheraxanthin, α- and β-carotene, were quantified by hplc-dad-ms in fourteen genotypes of wheat, barley and tritordeum harvested in czechia in 2014 and 2015. the highest carotenoid contents were found in yellow-grained tritordeum ht 439 (12.16μg/gdw), followed by blue-grained wheat v1-131-15 (7.46μg/gdw), and yellow-grained wheat ta 4024 (7.04μg/gdw) ...201828946328
effects of hmw-gs ax1 or dx2 absence on the glutenin polymerization and gluten micro structure of wheat (triticum aestivum l.).wheat (triticum aestivum l.) dough strength and extensibility are mainly determined by the polymerization of glutenin. the number of high-molecular-weight glutenin subunits (hmw-gs) differs in various wheat varieties due to the silencing of some genes. the effects of ax1 or dx2 subunit absence on glutenin polymerization, dough mixing properties and gluten micro structure were investigated with two groups of near-isogenic lines. the results showed that ax1 or dx2 absence decreased the accumulatio ...201828946321
genome-wide association mapping of loci for resistance to stripe rust in north american elite spring wheat germplasm.stripe rust, caused by puccinia striiformis f. sp. tritici, is a major yield-limiting foliar disease of wheat (triticum aestivum) worldwide. in this study, the genetic variability of elite spring wheat germplasm from north america was investigated to characterize the genetic basis of effective all-stage and adult plant resistance (apr) to stripe rust. a genome-wide association study was conducted using 237 elite spring wheat lines genotyped with an illumina infinium 90k single-nucleotide polymor ...201828952421
characterization of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen zymoseptoria tritici.the fungus zymoseptoria tritici is the causal agent of septoria tritici blotch (stb) disease of wheat leaves. zymoseptoria tritici secretes many functionally uncharacterized effector proteins during infection. here, we characterized a secreted ribonuclease (zt6) with an unusual biphasic expression pattern. transient expression systems were used to characterize zt6, and mutants thereof, in both host and non-host plants. cell-free protein expression systems monitored the impact of zt6 protein on f ...201828895153
density separation as a strategy to reduce the enzyme load of preharvest sprouted wheat and enhance its bread making quality.as preharvest sprouting of wheat impairs its use in food applications, postharvest solutions for this problem are required. due to the high kernel to kernel variability in enzyme activity in a batch of sprouted wheat, the potential of eliminating severely sprouted kernels based on density differences in nacl solutions was evaluated. compared to higher density kernels, lower density kernels displayed higher α-amylase, endoxylanase, and peptidase activities as well as signs of (incipient) protein, ...201828958551
label-free quantitative proteomic analysis of drought stress-responsive late embryogenesis abundant proteins in the seedling leaves of two wheat (triticum aestivum l.) genotypes.late embryogenesis abundant (lea) proteins are highly hydrophilic proteins with key roles in environmental stress responses. in this study, we performed the first survey of the lea proteome in seedling leaves from two wheat genotypes subjected to drought stress, i.e., shaanhe 6 (sh, drought-tolerant) and zhengyin 1 (zy, drought-sensitive). after isolating the lea subpopulation by treating total soluble proteins with heating combined with 1% trichloroacetic acid treatment that was assessed by wes ...201828982538
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