Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| effect of seed pelleting with biocontrol agents on growth and colonisation of roots of mungbean by root-infecting fungi. | mungbean (vigna radiata (l.) wilczek) is a leguminous pulse crop that is a major source of proteins, vitamins and minerals. root-infecting fungi produce severe plant diseases like root rot, charcoal rot, damping-off and stem rot. the soil-borne pathogens can be controlled by chemicals, but these chemicals have several negative effects. use of microbial antagonist such as fungi and bacteria is a safe, effective and eco-friendly method for the control of many soil-borne pathogens. biological contr ... | 2016 | 26619828 |
| streptomyces strains producing mitochondriotoxic antimycin a found in cereal grains. | reasons for mammalian cell toxicity observed in barley and spring wheat grains were sought. streptomyces sp. isolates from wheat and barley produced heat-stable methanol-soluble substances which inhibited the motility of exposed porcine spermatozoa used as a toxicity indicator. several barley isolates produced antimycin a (2 to 5 ng/mg wet wt of biomass), a macrolide antibiotic known to block oxygen utilization in mitochondria. the antimycin-producing isolates were members of the streptomyces al ... | 2016 | 26619316 |
| bacillus cereus ar156 activates pamp-triggered immunity and induces a systemic acquired resistance through a npr1-and sa-dependent signaling pathway. | induced resistance responses play a potent role in plant defense system against pathogen attack. bacillus cereus ar156 is a plant growth promoting rhizobacterium (pgpr) that installs induced systemic resistance (isr) to pseudomonas syringae pv. tomato (pst) in arabidopsis. here, we show that ar156 leaf infiltration enhances disease resistance in arabidopsis through the activation of a systemic acquired resistance (sar). pr1 protein expression and reactive oxygen species (ros) burst are strongly ... | 2016 | 26616055 |
| effects of electrolyzed oxidizing water on inactivation of bacillus subtilis and bacillus cereus spores in suspension and on carriers. | spores of some bacillus species are responsible for food spoilage and foodborne disease. these spores are highly resistant to various interventions and cooking processes. in this study, the sporicidal efficacy of acidic electrolyzed oxidizing (eo) water (aew) and slightly acidic eo water (saew) with available chlorine concentration (acc) of 40, 60, 80, 100, and 120 mg/l and treatment time for 1, 2, 3, 4, 5, and 6 min were tested on bacillus subtilis and bacillus cereus spores in suspension and o ... | 2016 | 26642381 |
| recovery and purification of chitosanase produced by bacillus cereus using expanded bed adsorption and central composite design. | this study presents a system for expanded bed adsorption for the purification of chitosanase from broth extract in a single step. a chitosanase-producing strain was isolated and identified as bacillus cereus c-01 and used to produce chitosanases. the expanded bed adsorption conditions for chitosanase purification were optimized statistically using streamline(tm) deae and a homemade column (2.6 × 30.0 cm). dependent variables were defined by the quality criteria purification factor (p) and enzyme ... | 2016 | 26638991 |
| formation of small transmembrane pores: an intermediate stage on the way to bacillus cereus non-hemolytic enterotoxin (nhe) full pores in the absence of nhea. | the non-hemolytic enterotoxin (nhe) of bacillus cereus is a three-partite toxin formed of the components nhea, -b and -c. pore formation and subsequent lysis of target cells caused by nhe is an orchestrated process comprising three steps: (i) formation of nheb/c oligomers in solution, (ii) attachment of the oligomers to the cell membrane, (iii) binding of nhea to the oligomers. the present study aimed to characterize the properties of the nheb/c complex and the fate of the target cell upon bindi ... | 2016 | 26654951 |
| detection of multiple resistances, biofilm formation and conjugative transfer of bacillus cereus from contaminated soils. | the purpose of this study was to detect microbial resistances to a set of antibiotics/pesticides (multi-resistance) within pesticide and antibiotic-contaminated alluvial soils and to identify the corresponding antibiotic resistance genes (args). to assess whether identified multi-resistant isolates are able to construct biofilms, several biofilm formation and conjugation experiments were conducted. out of 35 isolates, six strains were used for filter mating experiments. nine strains were identif ... | 2016 | 26650381 |
| the effects of citrus extract (citrox©) on the naturally occurring microflora and inoculated pathogens, bacillus cereus and salmonella enterica, in a model food system and the traditional greek yogurt-based salad tzatziki. | the antimicrobial effect of citrus extract (at 1 ml/kg [tc1] and 2 ml/kg [tc2]) on the naturally occurring microflora and inoculated pathogens (bacillus cereus and salmonella enterica, at ca. 6 log cfu/g) in the traditional greek yogurt-based salad tzatziki during storage under vacuum at 4 or 10 °c was examined. we also examined the effect of citrus extract (citrox(©)) against the two aforementioned pathogens in tryptic soy broth (tsb). of the two treatments, tc2 yielded the lowest yeast counts, ... | 2016 | 26678142 |
| lipase-catalyzed synthesis of palmitanilide: kinetic model and antimicrobial activity study. | enzymatic syntheses of fatty acid anilides are important owing to their wide range of industrial applications in detergents, shampoo, cosmetics, and surfactant formulations. the amidation reaction of mucor miehei lipase lipozyme im20 was investigated for direct amidation of triacylglycerol in organic solvents. the process parameters (reaction temperature, substrate molar ratio, enzyme amount) were optimized to achieve the highest yield of anilide. the maximum yield of palmitanilide (88.9%) was a ... | 2016 | 26672452 |
| antibacterial effects of alchornea cordifolia (schumach. and thonn.) müll. arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens. | the leaves, stems and roots of alchornea cordifolia (schumach. and thonn.) müll. arg. are used as traditional medicine in many african countries for the management of gastrointestinal, respiratory and urinary tract infections as well as for the treatment of wounds. | 2016 | 26724423 |
| effects of potential probiotic bacillus cereus en25 on growth, immunity and disease resistance of juvenile sea cucumber apostichopus japonicus. | this study was conducted to determine effects of potential probiotic bacillus cereus en25 (isolated from mud of sea cucumber culturing water bodies) on growth, immunity and disease resistance against vibrio splendidus infection in juvenile sea cucumbers apostichopus japonicus. animals were respectively fed diets with b. cereus en25 at 0 (control), 10(5), 10(7) and 10(9) cfu/g for 30 days. results showed that dietary b. cereus en25 had no significant effects on growth, total coelomocytes counts a ... | 2016 | 26723266 |
| butanol production under microaerobic conditions with a symbiotic system of clostridium acetobutylicum and bacillus cereus. | one major problem of abe (acetone, butanol and ethanol) fermentation is high oxygen sensitivity of clostridium acetobutylicum. currently, no single strain has been isolated or genetically engineered to produce butanol effectively under aerobic conditions. in our previous work, a symbiotic system tsh06 has been developed successfully by our group, and two strains, c. acetobutylicum tsh1 and bacillus cereus tsh2, were isolated from tsh06. | 2016 | 26762531 |
| microbiology of corneal wounds after cataract surgery: biofilm formation and antibiotic resistance patterns. | to evaluate the bacterial flora of corneal wounds at the end of cataract surgery before intracameral antibiotic use and to determine agents to treat postoperative endophthalmitis, the potential for biofilm formation, and antibiotic resistance. | 2016 | 26762493 |
| synthesis of sic/ag/cellulose nanocomposite and its antibacterial activity by reactive oxygen species generation. | we describe the synthesis of nanocomposites, based on nanofibers of silicon carbide, silver nanoparticles, and cellulose. silver nanoparticle synthesis was achieved with chemical reduction using hydrazine by adding two different surfactants to obtain a nanocomposite with silver nanoparticles of different diameters. determination of antibacterial activity was based on respiration tests. enzymatic analysis indicates oxidative stress, and viability testing was conducted using an epifluorescence mic ... | 2016 | 28335299 |
| bio-prospecting of cuttle fish waste and cow dung for the production of fibrinolytic enzyme from bacillus cereus ind5 in solid state fermentation. | the process parameters governing the production of fibrinolytic enzyme in solid state fermentation employing bacillus cereus ind5 and using cuttle fish waste and cow dung substrate were optimized. the ph value of the medium, moisture content, sucrose, casein and magnesium sulfate were considered for two-level full factorial design and ph, casein and magnesium sulfate were identified as the important factors for fibrinolytic enzyme production. central composite design was applied to investigate t ... | 2016 | 28330303 |
| in vitro cytotoxicity and antibacterial activity of silver-coated electrospun polycaprolactone/gelatine nanofibrous scaffolds. | the development of nano-sized scaffolds with antibacterial properties that mimic the architecture of tissue is one of the challenges in tissue engineering. in this study, polycaprolactone (pcl) and pcl/gelatine (ge) (70:30) nanofibrous scaffolds were fabricated using a less toxic and common solvent, formic acid and an electrospinning technique. nanofibrous scaffolds were coated with silver (ag) in different concentrations of silver nitrate (agno3) aqueous solution (1.25, 2.5, 5, and 10 %) by usi ... | 2016 | 28330282 |
| biodegradation of real petroleum wastewater by immobilized hyper phenol-tolerant strains of bacillus cereus in a fluidized bed bioreactor. | microbial bioremediation of petroleum wastewater by phenol-degrading-bacteria holds promise in circumventing the issue of petroleum-spill related pollution. herein, biodegradation of petroleum wastewater samples collected from oil refinery site was carried out in a fluidized bed bioreactor by ca-alginate immobilized biomass of phenol-degrading strains of bacillus cereus (akg1 mtcc9817 and akg2 mtcc9818). degradation performance of the system was evaluated by measuring the changes in chemical oxy ... | 2016 | 28330209 |
| isolation and characterization of bacterial endophytes of curcuma longa l. | fourteen endophytic bacterial isolates were isolated from the rhizome of curcuma longa l. were characterized on the basis of morphology, biochemical characteristics and 16s rrna gene sequence analysis. the isolates were identified to six strains namely bacillus cereus (ecl1), bacillus thuringiensis (ecl2), bacillus sp. (ecl3), bacillus pumilis (ecl4), pseudomonas putida (ecl5), and clavibacter michiganensis (ecl6). all the strains produced iaa and solubilized phosphate and only two strains produ ... | 2016 | 28330130 |
| production of γ-cyclodextrin by bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system. | aqueous two-phase system (atps) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. extractive bioconversion of gamma-cyclodextrin (γ-cd) from soluble starch with cyclodextrin glycosyltransferase (cgtase, ec 2.4.1.19) enzyme derived from bacillus cereus was evaluated using polyethylene glycol (peg)/potassium phosphate based on atps. the optimum condition was attained in the atps constituted of 30.0% (w/w) peg 3000 g/mol and 7. ... | 2016 | 26702953 |
| comparative elimination of dimethyl disulfide by maifanite and ceramic-packed biotrickling filters and their response to microbial community. | unpleasant odor emissions have traditionally occupied an important role in environmental concern. in this paper, twin biotrickling filters (btfs) packed with different packing materials, seeded with bacillus cereus gigan2, were successfully constructed to purify gaseous dimethyl disulfide (dmds). the maifanite-packed btf showed superior biodegradation capability to the ceramic-packed counterpart in terms of removal efficiency and elimination capacity under similar conditions. at an empty bed res ... | 2016 | 26702514 |
| biocatalytic resolution of rac-α-ethyl-2-oxo-pyrrolidineacetic acid methyl ester by immobilized recombinant bacillus cereus esterase. | a new esterase-producing strain (bacillus cereus wzz001) which exhibiting high hydrolytic activity and excellent enantioselectivity on rac-α-ethyl-2-oxo-pyrrolidineacetic acid methyl ester (r, s-1) has been isolated from soil sample by our laboratory. in this study, the stereoselective hydrolysis of (r, s-1) was performed using the recombinant bacillus cereus esterase which expressed in escherichia coli bl21 (de3). under the optimized conditions of ph 8.0, 35 °c, and concentration of substrate 4 ... | 2016 | 26695776 |
| rapid detection of cronobacter sakazakii by real-time pcr based on the cgca gene and taqman probe with internal amplification control. | cronobacter sakazakii is a severe virulent strain that is frequently detected in powdered infant formula (pif). therefore, it is necessary to develop a fast and specific detection method. the specificity of our newly developed quantitative real-time pcr (qrt-pcr) was validated with dna from 46 strains. among them, 12 c. sakazakii strains were correctly amplified, whereas no positive florescent signal was observed from 34 nontarget controls. the detection limit of c. sakazakii was about 110 cfu/m ... | 2016 | 26751178 |
| advanced molecular diagnostic techniques for detection of food-borne pathogens; current applications and future challenges. | the elimination of disease-causing microbes from the food supply is a primary goal and this review deals with the overall techniques availavle for detection of food-borne pathogens. now-a-days conventional methods are replaced by advanced methods like biosensors, nucleic acid-based tests (nat) and different pcr based techniques used in molecular biology to identify specific pathogens. bacillus cereus, staphylococcus aureus, proteus vulgaris, escherichia coli, campylobacter, listeria monocytogene ... | 2016 | 26745757 |
| transcriptome analysis of bacillus thuringiensis spore life, germination and cell outgrowth in a vegetable-based food model. | toxigenic species belonging to bacillus cereus sensu lato, including bacillus thuringiensis, cause foodborne outbreaks thanks to their capacity to survive as spores and to grow in food matrixes. the goal of this work was to assess by means of a genome-wide transcriptional assay, in the food isolate b. thuringiensis uc10070, the gene expression behind the process of spore germination and consequent outgrowth in a vegetable-based food model. scanning electron microscopy and energy dispersive x-ray ... | 2016 | 26742618 |
| safety of street-vended soy wara in nigeria. | soy wara is a common ready-to-eat food whose production and sale are currently unregulated. microbiological sampling indicated that 21% of the samples had standard plate counts exceeding 100,000 cfu/g, and 14% had staphylococcus aureus counts higher than 100,000 cfu/g. the occurrence of s. aureus at these levels can result in food poisoning. listeria monocytogenes was isolated in 14.4% of the samples, although the counts were generally low, typically <1,000 cfu/g. although counts of l. monocytog ... | 2016 | 26735046 |
| microbiological analysis of rice cake processing in korea. | this study was conducted to evaluate the microbial contamination in rice cake materials and products during processing and in the operation environment in nonhazard analysis [and] critical control point factories. furthermore, the environmental health of the processing facilities and the bacterial and fungal contamination on the workers' hands were investigated. pour plate methods were used for enumeration of aerobic plate count (apc), yeast and molds (ym), bacillus cereus, staphylococcus aureus ... | 2016 | 26735044 |
| antimicrobial effects of marine algal extracts and cyanobacterial pure compounds against five foodborne pathogens. | the marine environment is a proven source of structurally complex and biologically active compounds. in this study, the antimicrobial effects of a small collection of marine-derived extracts and isolates, were evaluated against 5 foodborne pathogens using a broth dilution assay. results demonstrated that algal extracts from padina and ulva species and cyanobacterial compounds antillatoxin b, laxaphycins a, b and b3, isomalyngamide a, and malyngamides c, i and j showed antimicrobial activity agai ... | 2016 | 26775951 |
| genomic characterization and comparison of seven myoviridae bacteriophage infecting bacillus thuringiensis. | bacillus thuringiensis kurstaki, a bacterium that is a source of biopesticides and a safe simulant for pathogenic bacillus species, was used to isolate seven unique bacteriophages. the phage genomes were sequenced and ranged in size from 158,100 to 163,019 bp encoding 290-299 genes, and the gc content of ~38% was similar to that of the host bacterium. all phages had terminal repeats 2-3 kb long. three of the phages encoded trnas and three contained a self-splicing intron in the dna polymerase ge ... | 2016 | 26773385 |
| isolation, purification and characterization of antimicrobial protein from seedlings of bauhinia purpurea l. | a novel antimicrobial protein was purified from the seedlings of bauhinia purpurea by sequential procedures entailing ammonium sulfate precipitation, cation exchange chromatography, preparative native-page and a yield of 2.7% was obtained from the crude extract. the purified antimicrobial protein appeared as a single protein band on sds-page with the molecular mass of 20.9 kda. purified antimicrobial protein exhibited a potent antimicrobial activity against both gram-positive and gram-negative b ... | 2016 | 26780468 |
| use of peripheral parenteral nutrition solutions as a risk factor for bacillus cereus peripheral venous catheter-associated bloodstream infection at a japanese tertiary care hospital: a case-control study. | the risk factors are unclear for peripheral venous catheter-associated bloodstream infections (pvcbsis) caused by bacillus cereus. we aimed to examine for these risk factors in patients with b. cereus pvcbsi by conducting a 2-year case-control study in a large teaching hospital. we analyzed all adult cases of b. cereus pvcbsi (37 patients) and 180 controls who were randomly selected from among patients who had a pvc in place for at least 2 days. multivariate analysis using a conditional logistic ... | 2016 | 26902219 |
| a rapid and simple real-time pcr assay for detecting foodborne pathogenic bacteria in human feces. | a rapid, simple method for detecting foodborne pathogenic bacteria in human feces is greatly needed. here, we examined the efficacy of a method that employs a combination of a commercial pcr master mix, which is insensitive to pcr inhibitors, and a dna extraction method which used sodium dodecyl benzene sulfonate (sdbs), and tween 20 to counteract the inhibitory effects of sdbs on the pcr assay. this method could detect the target genes (stx1 and stx2 of enterohemorrhagic escherichia coli, inva ... | 2016 | 26902211 |
| surface modification of a polyethylene film for anticoagulant and anti-microbial catheter. | a functional anticoagulant and anti-bacterial coating for polyethylene (pe) films is described. the stepwise preparation of this nanocomposite surface coating involves o2 plasma etching of pe film, carbodiimide coupling of cysteamine to the etched pe film, binding of ag to sulfhydryl groups of cysteamine, and assembly of heparin capped agnps on the pe film. the nanocomposite film and its components were characterized by (1)h-nuclear magnetic resonance spectroscopy, attenuated total reflectance-f ... | 2016 | 26900340 |
| fabrication of an electrochemical dna-based biosensor for bacillus cereus detection in milk and infant formula. | this paper describes fabrication of a dna-based au-nanoparticle modified pencil graphite electrode (pge) biosensor for detection of bacillus cereus, causative agent of two types of food-borne disease, i.e., emetic and diarrheal syndrome. the sensing element of the biosensor was comprised of gold nanoparticles (gnps) self-assembled with single-stranded dna (ssdna) of nhea gene immobilized with thiol linker on the gnps modified pge. the size, shape and dispersion of the gnps were characterized by ... | 2016 | 26896793 |
| bacillus licheniformis trehalose-6-phosphate hydrolase structures suggest keys to substrate specificity. | trehalose-6-phosphate hydrolase (trea) belongs to glycoside hydrolase family 13 (gh13) and catalyzes the hydrolysis of trehalose 6-phosphate (t6p) to yield glucose and glucose 6-phosphate. the products of this reaction can be further metabolized by the energy-generating glycolytic pathway. here, crystal structures of bacillus licheniformis trea (bltrea) and its r201q mutant complexed with p-nitrophenyl-α-d-glucopyranoside (r201q-ppng) are presented at 2.0 and 2.05 å resolution, respectively. the ... | 2016 | 26894535 |
| bacteria-based polythene degradation products: gc-ms analysis and toxicity testing. | polythene degradation leads to the production of various by-products depending upon the type of degradation process. the polythene degradation products (pedp) in the culture supernatant of the two bacteria (lysinibacillus fusiformis strain vasb14/wl and bacillus cereus strain vasb1/ts) were analyzed with gc-ms technique. the major by-products in the pedp in the culture supernatant of l. fusiformis strain vasb14/wl (1,2,3,4 tetra methyl benzene) and b. cereus strain vasb1/ts (1,2,3 trimethyl benz ... | 2016 | 26888528 |
| association of genotyping of bacillus cereus with clinical features of post-traumatic endophthalmitis. | bacillus cereus is the second most frequent cause of post-traumatic bacterial endophthalmitis. although genotyping of b. cereus associated with gastrointestinal infections has been reported, little is known about the b. cereus clinical isolates associated with post-traumatic endophthalmitis. this is largely due to the limited number of clinical strains available isolated from infected tissues of patients with post-traumatic endophthalmitis. in this study, we report successful isolation of twenty ... | 2016 | 26886446 |
| investigation of equilibrium and kinetics of cr(vi) adsorption by dried bacillus cereus using response surface methodology. | in this study, response surface methodology (rsm) based on three-variable-five-level central composite rotatable design was used to analyze the effects of combined and individual operating parameters (biomass dose, initial concentration of cr(vi) and ph) on the cr(vi) adsorption capacity of dried bacillus cereus. a quadratic polynomial equation was obtained to predict the adsorbed cr(vi) amount. analysis of variance showed that the effect of biomass dose was the key factor in the removal of cr(v ... | 2016 | 26877045 |
| the pulsed light inactivation of veterinary relevant microbial biofilms and the use of a rtpcr assay to detect parasite species within biofilm structures. | the presence of pathogenic organisms namely parasite species and bacteria in biofilms in veterinary settings, is a public health concern in relation to human and animal exposure. veterinary clinics represent a significant risk factor for the transfer of pathogens from housed animals to humans, especially in cases of wound infection and the shedding of faecal matter. this study aims to provide a means of detecting veterinary relevant parasite species in bacterial biofilms, and to provide a means ... | 2016 | 26862516 |
| uptake and levels of the antibiotic berberine in individual dormant and germinating clostridium difficile and bacillus cereus spores as measured by laser tweezers raman spectroscopy. | spores of clostridium difficile and bacillus cereus are major causes of nosocomial diarrhoea and foodborne disease. our aim was to measure the dynamics of the uptake of the antibiotic berberine by individual germinating spores and the levels of berberine accumulated in germinated spores. | 2016 | 26861569 |
| antioxidant and dna damage protecting activity of exopolysaccharides from the endophytic bacterium bacillus cereus sz1. | an endophytic bacterium was isolated from the chinese medicinal plant artemisia annua l. the phylogenetic and physiological characterization indicated that the isolate, strain sz-1, was bacillus cereus. the endophyte could produce an exopolysaccharide (eps) at 46 mg/l. the 1,1-diphenyl-2-picrylhydracyl (dpph) radical scavenging activity of the eps reached more than 50% at 3-5 mg/ml. the eps was also effective in scavenging superoxide radical in a concentration dependent fashion with an ec50 valu ... | 2016 | 26861269 |
| understanding the antimicrobial activity behind thin- and thick-rolled copper plates. | the aim of this study was to compare the antibacterial properties of the surfaces of copper plates that were rolled to a thickness of 25 and 100 μm. differences in topology of 25- and 100-μm-thick copper plates were studied using scanning electron microscopy (sem), atomic force microscopy (afm), and x-ray diffraction (xrd). antibacterial activity of the copper surfaces was tested against strains of staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, listeria m ... | 2016 | 26860943 |
| biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat. | biosynthesis of agnps by 37 different bacterial soil isolates was done and confirmed through visible spectrophotometry. fifteen isolates were identified and two of them with the highest ability of agnps production were used for transmission electron microscopy (tem) and x-ray diffraction (xrd) tests. mtt assay for both of the obtained agnps was run and after determination their ic50s, two different toxic and nontoxic doses of each agnps solution were chosen for wound healing assay. forty eight r ... | 2016 | 26854241 |
| effect of fermented broth from lactic acid bacteria on pathogenic bacteria proliferation. | in this study, the effect that 5 fermented broths of lactic acid bacteria (lab) strains have on the viability or proliferation and adhesion of 7 potentially pathogenic microorganisms was tested. the fermented broth from lactococcus lactis c660 had a growth inhibitory effect on escherichia coli k92 that reached of 31%, 19% to pseudomonas fluorescens, and 76% to staphylococcus epidermidis. the growth of staph. epidermidis was negatively affected to 90% by lc. lactis 11454 broth, whereas the growth ... | 2016 | 26851857 |
| detection and quantification of viable bacillus cereus group species in milk by propidium monoazide quantitative real-time pcr. | the bacillus cereus group includes important spore-forming bacteria that present spoilage capability and may cause foodborne diseases. these microorganisms are traditionally evaluated in food using culturing methods, which can be laborious and time-consuming, and may also fail to detect bacteria in a viable but nonculturable state. the purpose of this study was to develop a quantitative real-time pcr (qpcr) combined with a propidium monoazide (pma) treatment to analyze the contamination of uht m ... | 2016 | 26830746 |
| antagonistics against pathogenic bacillus cereus in milk fermentation by lactobacillus plantarum zdy2013 and its anti-adhesion effect on caco-2 cells against pathogens. | lactobacillus plantarum zdy2013 is a potential probiotic isolated from fermented bean acid. in this study, we aimed to evaluate the in vitro antimicrobial activity of this organism against bacillus cereus in milk fermentation, the antiadhesion ability on intestinal epithelial cells, as well as its ability to abrogate the cytotoxic effect and expression levels of genes. we found no antimicrobial activity produced by l. plantarum once the ph was adjusted to 6.0 and 7.0. the ph decreased continuous ... | 2016 | 26830743 |
| isolation and characterization of spore-forming bacilli (sfb) from shepherd's purse (capsella bursa-pastoris). | shepherd's purse (capsella bursa-pastoris), native to europe, is commonly consumed fresh and sometimes inadequately washed before consumption in korea. the objective of this study was to characterize isolates of spore-forming bacilli (sfb) in samples of fresh shepherd's purse. three genera were identified: bacillus (9 species), paenibacillus (3 species), and brevibacillus (1 species). none of the genes of the hemolysin bl (hbl) and nonhemolytic enterotoxin (nhe) complexes, or of the emetic toxin ... | 2016 | 26822957 |
| antibacterial and synergistic activity of pentacyclic triterpenoids isolated from alstonia scholaris. | (1) background: alstonia scholaris (apocynaceae) is an important medicinal plant that has been historically used in "dai" ethnopharmacy to treat infectious diseases in china. although various pharmacological activities have been reported, the antimicrobial constitutes of a. scholaris have not yet been identified. the objective of this study is to evaluate the antibacterial constitutes from the leaf extract of a. scholaris and to assess the synergistic effects of isolated compounds with antibioti ... | 2016 | 26821000 |
| in vitro antibacterial activity of selected medicinal plants traditionally used in vietnam against human pathogenic bacteria. | medicinal plants are widely used for the treatment of different infectious diseases. infectious diseases caused by bacteria have a large impact on public health. this study aimed to determine the in vitro antibacterial activity of the medicinal plants traditionally used in vietnam against the bacterial strains associated with infectious diseases. | 2016 | 26819218 |
| characterization and exposure assessment of emetic bacillus cereus and cereulide production in food products on the dutch market. | the emetic toxin cereulide, which can be produced by bacillus cereus, can be the cause of food poisoning upon ingestion by the consumer. the toxin causes vomiting and is mainly produced in farinaceous food products. this article includes the prevalence of b. cereus and of cereulide in food products in the netherlands, a characterization of b. cereus isolates obtained, cereulide production conditions, and a comparison of consumer exposure estimates with those of a previous exposure assessment. fo ... | 2016 | 26818983 |
| antimicrobial compounds from endophytic streptomyces sp. bcc72023 isolated from rice (oryza sativa l.). | an endophytic actinomycete strain bcc72023 was isolated from rice (oryza sativa l.) and identified as the genus streptomyces, based on phenotypic, chemotaxonomic and 16s rrna gene sequence analyses. the strain showed 99.80% similarity compared with streptomyces samsunensis m1463(t). chemical investigation led to the isolation of three macrolides, efomycins m (1), g (2) and oxohygrolidin (3), along with two polyethers, abierixin (4) and 29-o-methylabierixin (5). to our knowledge, this is the firs ... | 2016 | 26809052 |
| evaluation of probiotic properties of lactobacillus plantarum wlpl04 isolated from human breast milk. | lactobacillus plantarum wlpl04, a specific strain isolated from human breast milk, was investigated for its survival capacity (acid and bile salt tolerance, survival in simulated gastrointestinal tract, inhibition of pathogens, antibiotic susceptibility, yield of exopolysaccharides) and probiotic properties (antiadhesion of pathogens, protection from harmful effect of sodium dodecyl sulfate, and antiinflammatory stress on caco-2 cells). the results showed that lb. plantarum wlpl04 had broad-spec ... | 2016 | 26805974 |
| characterization of antimicrobial lipopeptides produced by bacillus sp. lm7 isolated from chungkookjang, a korean traditional fermented soybean food. | a wild-type microorganism exhibiting antimicrobial activities was isolated from the korean traditional fermented soybean food chungkookjang and identified as bacillus sp. lm7. during its stationary growth phase, the microorganism secreted an antimicrobial substance, which we partially purified using a simple two-step procedure involving ammonium sulfate precipitation and heat treatment. the partially purified antimicrobial substance, anti-lm7, was stable over a broad ph range (4.0-9.0) and at te ... | 2016 | 26803269 |
| diversity of sugar acceptor of glycosyltransferase 1 from bacillus cereus and its application for glucoside synthesis. | glycosyltransferase 1 from bacillus cereus (bcgt1) catalyzes the transfer of a glucosyl moiety from uridine diphosphate glucose (udp-glucose) to various acceptors; it was expressed and characterized. the specificity of acceptors was found to be broad: more than 20 compounds classified into o-, s-, and n-linkage glucosides can be prepared with bcgt1 catalysis. based on this work, we conclude that the corresponding acceptors of these compounds must possess the following features: (1) the acceptors ... | 2016 | 26795959 |
| α,β-dibromochalcone derivatives--synthesis and antimicrobial activity. | to obtain some chalcones and their dibrominated analogues and to evaluate their antimicrobial potential. | 2016 | 26793867 |
| noninvasive ph monitoring for bacterial detection in platelet concentrates. | bacterial contamination of platelet concentrates (pcs) remains the prevalent posttransfusion infectious risk. the ph safe system, a noninvasive method used to measure ph of pc for quality control, was evaluated herein as a rapid method to detect bacterial contamination in pcs. | 2016 | 27028108 |
| antibacterial properties and effects of fruit chilling and extract storage on antioxidant activity, total phenolic and anthocyanin content of four date palm (phoenix dactylifera) cultivars. | phoenix dactylifera or date palm fruits are reported to contain natural compounds that exhibit antioxidant and antibacterial properties. this research aimed to study the effect of fruit chilling at 4 °c for 8 weeks, extract storage at -20 °c for 5 weeks, and extraction solvents (methanol or acetone) on total phenolic content (tpc), antioxidant activity and antibacterial properties of saudi arabian p. dactylifera cv mabroom, safawi and ajwa, as well as iranian p. dactylifera cv mariami. the stora ... | 2016 | 27023514 |
| optimization of peptide nucleic acid fluorescence in situ hybridization (pna-fish) for the detection of bacteria: the effect of ph, dextran sulfate and probe concentration. | fluorescence in situ hybridization (fish) is a molecular technique widely used for the detection and characterization of microbial populations. fish is affected by a wide variety of abiotic and biotic variables and the way they interact with each other. this is translated into a wide variability of fish procedures found in the literature. the aim of this work is to systematically study the effects of ph, dextran sulfate and probe concentration in the fish protocol, using a general peptide nuclei ... | 2016 | 27021959 |
| adhesion and removal kinetics of bacillus cereus biofilms on ni-ptfe modified stainless steel. | biofilm control remains a challenge to food safety. a well-studied non-fouling coating involves codeposition of polytetrafluoroethylene (ptfe) during electroless plating. this coating has been reported to reduce foulant build-up during pasteurization, but opportunities remain in demonstrating its efficacy in inhibiting biofilm formation. herein, the initial adhesion, biofilm formation, and removal kinetics of bacillus cereus on ni-ptfe-modified stainless steel (ss) are characterized. coatings lo ... | 2016 | 27020838 |
| erratum to: seasonal trend and clinical presentation of bacillus cereus bloodstream infection: association with summer and indwelling catheter. | bacillus cereus, an opportunistic pathogen, can cause fatal infection. however, b. cereus bloodstream infections (bsis) have not been well characterised. from 2008 to 2013, b. cereus isolates from all of the specimens and patients with b. cereus bsis were identified. environmental samples were collected to detect b. cereus contamination. we also characterised the clinical presentation of b. cereus bsi through analyses of risk factors for bsi and mortality. a total of 143 clinical b. cereus isola ... | 2016 | 27010814 |
| identification of cereulide-producing bacillus cereus by nucleic acid chromatography and reverse transcription real-time pcr. | rna extracts were analyzed with a nucleic acid sequence-based amplification (nasba) - nucleic acid chromatography and a reverse transcription-quantitative pcr assay (rt-qpcr) based on the taqman probe for identification of cereulide-producing bacillus cereus. all 100 emetic b. cereus strains were found to give positive results, but 50 diarrheal b. cereus strains and other bacterial species showed negative results in the nasba-chromatography. that is, the assay could selectively identify the emet ... | 2016 | 27009509 |
| antibacterial activity and action mechanism of the essential oil from enteromorpha linza l. against foodborne pathogenic bacteria. | foodborne illness and disease caused by foodborne pathogenic bacteria is continuing to increase day by day and it has become an important topic of concern among various food industries. many types of synthetic antibacterial agents have been used in food processing and food preservation; however, they are not safe and have resulted in various health-related issues. therefore, in the present study, essential oil from an edible seaweed, enteromorpha linza (aeo), was evaluated for its antibacterial ... | 2016 | 27007365 |
| synthesis and evaluation of ester derivatives of 10-hydroxycanthin-6-one as potential antimicrobial agents. | as part of our continuing research on canthin-6-one antimicrobial agents, a new series of ester derivatives of 10-hydroxycanthin-6-one were synthesized using a simple and effective synthetic route. the structure of each compound was characterized by nmr, esi-ms, ft-ir, uv, and elemental analysis. the antimicrobial activity of these compounds against three phytopathogenic fungi (alternaria solani, fusarium graminearum, and fusarium solani) and four bacteria (bacillus cereus, bacillus subtilis, ra ... | 2016 | 27007362 |
| phytochemical analysis, antimicrobial and antioxidant activities of euphorbia golondrina l.c. wheeler (euphorbiaceae juss.): an unexplored medicinal herb reported from cameroon. | this study aimed at determining the phytochemical constituents of euphorbia golondrina l.c. wheeler, an alien invasive medicinal herb that is used for the treatment of gastroenteritis related ailments, diabetes, conjunctivitis, gastritis, enterocolitis, tonsillitis, vaginitis, hemorrhoids, prostatism, warts and painful swellings by the mundani people of the mount bambouto caldera in southwestern cameroon, and to evaluate its in vitro antimicrobial and antioxidant activities. susceptibility testi ... | 2016 | 27006873 |
| secreted compounds of the probiotic bacillus clausii strain o/c inhibit the cytotoxic effects induced by clostridium difficile and bacillus cereus toxins. | although the use of probiotics based on bacillus strains to fight off intestinal pathogens and antibiotic-associated diarrhea is widespread, the mechanisms involved in producing their beneficial effects remain unclear. here, we studied the ability of compounds secreted by the probiotic bacillus clausii strain o/c to counteract the cytotoxic effects induced by toxins of two pathogens, clostridium difficile and bacillus cereus, by evaluating eukaryotic cell viability and expression of selected gen ... | 2016 | 27001810 |
| evaluation of bacterial contamination of dental unit waterlines and use of a newly designed measurement device to assess retraction of a dental chair unit. | dental unit waterline (duwl) output water is delivered through instruments of a dental chair unit (dcu) to irrigate and cool teeth. however, these waterlines can be heavily contaminated with bacteria. | 2016 | 27000421 |
| shelf life of donkey milk subjected to different treatment and storage conditions. | the aim of this study was to investigate the effects of different treatment conditions on microbiological indicators of donkey milk hygiene and their evolution during shelf life at 4 and 12°c from 3 to 30d, simulating a farm-scale pasteurization and packing system. four treatment conditions were tested: no treatment (raw milk), pasteurization (65°c × 30 min), high-pressure processing (hpp), and pasteurization plus hpp. the microbiological quality of the raw donkey milk investigated was not optim ... | 2016 | 26995124 |
| purification and characterization of plantaricin jla-9: a novel bacteriocin against bacillus spp. produced by lactobacillus plantarum jla-9 from suan-tsai, a traditional chinese fermented cabbage. | bacteriocins are ribosomally synthesized peptides with antimicrobial activity produced by numerous bacteria. a novel bacteriocin-producing strain, lactobacillus plantarum jla-9, isolated from suan-tsai, a traditional chinese fermented cabbage, was screened and identified by its physiobiochemical characteristics and 16s rdna sequence analysis. a new bacteriocin, designated plantaricin jla-9, was purified using butanol extraction, gel filtration, and reverse-phase high-performance liquid chromatog ... | 2016 | 26985692 |
| formation and biopharmaceutical characterization of electrospun pvp mats with propolis and silver nanoparticles for fast releasing wound dressing. | antibacterial, antiviral, antifungal, antioxidant, anti-inflammatory, and anticancer activities of propolis and its ability to stimulate the immune system and promote wound healing make it a proper component for wound dressing materials. silver nanoparticles are recognized to demonstrate strong antiseptic and antimicrobial activity; thus, it also could be considered in the development of products for wound healing. combining propolis and silver nanoparticles can result in improved characteristic ... | 2016 | 26981531 |
| assessment of the bacterial contamination of hand air dryer in washrooms. | the present study was carried out, using standard techniques, to identify and count the bacterial contamination of hand air dryers, used in washrooms. bacteria were isolated from the air flow, outlet nozzle of warm air dryers in fifteen air dryers used in these washrooms. bacteria were found to be relatively numerous in the air flows. bacterially contaminated air was found to be emitted whenever a warm air dryer was running, even when not being used for hand drying. our investigation shows that ... | 2016 | 26981009 |
| development of a double-antibody sandwich elisa for rapid detection of bacillus cereus in food. | bacillus cereus is increasingly recognized as one of the major causes of food poisoning in the industrialized world. in this paper, we describe a sensitive double-antibody sandwich enzyme-linked immunosorbent assay (elisa) that was developed for rapid detection of b. cereus in food to minimize the risk of contamination. the polyclonal antibody (pab) and monoclonal antibodies (mabs) specific to b. cereus were generated from rabbit antiserum and mouse ascites, respectively, using the octanoic acid ... | 2016 | 26976753 |
| recombinant broad-range phospholipase c from listeria monocytogenes exhibits optimal activity at acidic ph. | the broad-range phospholipase c (plc) from listeria monocytogenes has been expressed using an intein expression system and characterized. this zinc metalloenzyme, similar to the homologous enzyme from bacillus cereus, targets a wide range of lipid substrates. with monomeric substrates, the length of the hydrophobic acyl chain has significant impact on enzyme efficiency by affecting substrate affinity (km). based on a homology model of the enzyme to the b. cereus protein, several active site resi ... | 2016 | 26976751 |
| recombinant thiopeptides containing noncanonical amino acids. | thiopeptides are a subclass of ribosomally synthesized and posttranslationally modified peptides (ripps) with complex molecular architectures and an array of biological activities, including potent antimicrobial activity. here we report the generation of thiopeptides containing noncanonical amino acids (ncaas) by introducing orthogonal amber suppressor aminoacyl-trna synthetase/trna pairs into a thiocillin producer strain of bacillus cereus .we demonstrate that thiopeptide variants containing nc ... | 2016 | 26976568 |
| application of mathematical models for bacterial inactivation curves using hypericin-based photosensitization. | the aim of this study was to evaluate which of the three mathematical models (weibull, geeraerd and logistic) makes the best approximation for hypericin-based photosensitization bacterial inactivation. | 2016 | 26972682 |
| new coumarin derivatives from ferula pseudalliacea with antibacterial activity. | in this study, the antibacterial activity of disesquiterpene coumarin and sesquiterpene coumarins obtained from ferula pseudalliacea roots was evaluated by determination of minimum inhibitory concentration using the broth micro-dilution method against seven pathogenic bacterial strains (staphylococcus aureus atcc 25,923, vancomycin resistant clinical strain of enterococcus faecium, bacillus cereus ptcc 1015, escherichia coli atcc 25,922, pseudomonas aeruginosa ptcc 1430, clinical strain of klebs ... | 2016 | 26953526 |
| four new carbazole alkaloids from murraya koenigii that display anti-inflammatory and anti-microbial activities. | in our present study, four new, designated as murrayakonine a-d (), along with 18 known carbazole alkaloids were isolated from chcl3 : meoh (1 : 1) crude extracts of the stems and leaves of murraya koenigii (linn.) spreng. the structures of the all isolated compounds were characterized by analysis of hr-esi-ms and nmr (1d and 2d spectroscopy) results, and comparison of their data with the literature data. for the first time, all the isolates were evaluated for their anti-inflammatory activities, ... | 2016 | 26947457 |
| the effect of storage temperature and duration on the microbial quality of bulk tank milk. | the dairy industry in ireland is currently undergoing a period of expansion and, as a result, it is anticipated that milk may be stored in bulk tanks on-farm for periods greater than 48 h. the objective of this study was to investigate the effects of storage temperature and duration on microbial quality of bulk tank milk when fresh milk is added to the bulk tank twice daily. bulk tank milk stored at 3 temperatures was sampled at 24-h intervals during storage periods of 0 to 96 h. bulk tank milk ... | 2016 | 26947309 |
| complete genome sequence of the cold-active bacteriophage vmy22 from bacillus cereus. | the cold-active bacteriophage vmy22, belonging to the podoviridae family, was isolated from mingyong glacier in china. sequence analysis revealed that the genome is 18,609 bp long, with an overall g + c content of 36.4 mol%, and 25 open reading frames (orfs). the sequence contains 46 potential promoters, 6 transcription terminators, and no trnas. most of the orfs show a high degree of similarity to b103 (nc_004165). two noteworthy findings were made. first, one of the predicted proteins, orf 19, ... | 2016 | 26941234 |
| a new benzopyrans derivatives from a mangrove-derived fungus penicillium citrinum from the south china sea. | one new benzopyran derivative (2r(*),4r(*))-3,4-dihydro-5-methoxy-2-methyl-2h-1-benzopyran-4-ol (1), together with five known compounds (2-6), were obtained from the etoac extract of the endophytic fungus penicillium citrinum hl-5126 isolated from the mangrove brguiera sexangula var. rhynchopetala collected in the south china sea. their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. all compounds were evaluated for their antibacterial activities. compoun ... | 2016 | 26930107 |
| eco-friendly and facile integrated biological-cum-photo assisted electrooxidation process for degradation of textile wastewater. | the present article reports an integrated treatment method viz biodegradation followed by photo-assisted electrooxidation, as a new approach, for the abatement of textile wastewater. in the first stage of the integrated treatment scheme, the chemical oxygen demand (cod) of the real textile effluent was reduced by a biodegradation process using hydrogels of cellulose-degrading bacillus cereus. the bio-treated effluent was then subjected to the second stage of the integrated scheme viz indirect el ... | 2016 | 26921849 |
| isolation and structural elucidation of brevibacillin, an antimicrobial lipopeptide from brevibacillus laterosporus that combats drug-resistant gram-positive bacteria. | a new environmental bacterial strain exhibited strong antimicrobial characteristics against methicillin-resistant staphylococcus aureus, vancomycin-resistant strains of enterococcus faecalis and lactobacillus plantarum, and other gram-positive bacteria. the producer strain, designated osy-i1, was determined to be brevibacillus laterosporusvia morphological, biochemical, and genetic analyses. the antimicrobial agent was extracted from cells of osy-i1 with isopropanol, purified by high-performance ... | 2016 | 26921428 |
| bacteriocinogenic bacteria isolated from raw goat milk and goat cheese produced in the center of méxico. | currently, there are few reports on the isolation of microorganisms from goat milk and goat cheese that have antibacterial activity. in particular, there are no reports on the isolation of microorganisms with antibacterial activity from these products in central mexico. our objective was to isolate bacteria, from goat products, that synthesized antimicrobial peptides with activity against a variety of clinically significant bacteria. we isolated and identified lactobacillus rhamnosus, l. plantar ... | 2016 | 27407294 |
| integrative approach for producing hydrogen and polyhydroxyalkanoate from mixed wastes of biological origin. | in this study, an integrative approach to produce biohydrogen (h2) and polyhydroxyalkanoates (pha) from the wastes of biological origin was investigated. a defined set of mixed cultures was used for hydrolysis and the hydrolysates were used to produce h2. the effluent from h2 production stage was used for pha production. under batch culture, a maximum of 62 l h2/kg of pure potato peels (total solid, ts 2 %, w/v) and 54 l h2/kg of mixed biowastes (mbw1) was recorded. using effluent from the h2 pr ... | 2016 | 27407293 |
| low-cost and eco-friendly green synthesis of silver nanoparticles using prunus japonica (rosaceae) leaf extract and their antibacterial, antioxidant properties. | low cost and eco-friendly green synthesis of silver nanoparticles (agnps) from silver nitrate (agno3) using prunus japonica leaves extract as reducing agent by a simple method at room temperature. the biosynthesized nanoparticles (nps) were characterized by uv-vis, tunneling electron microscopy (hr-tem), scanning electron microscopy (sem) coupled with x-ray energy dispersive spectrophotometer (edax), x-ray diffraction (xrd) and fourier transform infrared spectroscopy (ftir). in uv-vis spectrosco ... | 2016 | 27396523 |
| common mechanism of cross-resistance development in pathogenic bacteria bacillus cereus against alamethicin and pediocin involves alteration in lipid composition. | to understand the mechanism of development of cross-resistance in food pathogen bacillus cereus against an antimicrobial peptide pediocin and antibiotic alamethicin, the present study was designed. pediococcus pentosaceus was taken as a source of pediocin, and it was purified by ammonium sulphate precipitation followed by cation exchange chromatography with 14.01-fold purity and 14.4 % recovery. b. cereus strains alamethicin-resistant strains (ic50 3.23 µg/ml) were selected from sensitive popula ... | 2016 | 27378130 |
| comparative genomics of iron-transporting systems in bacillus cereus strains and impact of iron sources on growth and biofilm formation. | iron is an important element for bacterial viability, however it is not readily available in most environments. we studied the ability of 20 undomesticated food isolates of bacillus cereus and two reference strains for capacity to use different (complex) iron sources for growth and biofilm formation. studies were performed in media containing the iron scavenger 2,2-bipyridine. transcriptome analysis using b. cereus atcc 10987 indeed showed upregulation of predicted iron transporters in the prese ... | 2016 | 27375568 |
| combined effect of anaerobiosis, low ph and cold temperatures on the growth capacities of psychrotrophic bacillus cereus. | psychrotrophic strains of the foodborne pathogen bacillus cereus can multiply during the refrigerated storage of food products. the aim of this study was to determine the impact of anaerobiosis on the growth of two psychrotrophic b. cereus strains exposed to acidic ph at a cold temperature in a laboratory medium. at 10 °c, growth occurred at ph values equal to or higher than 5.7 during anaerobiosis, whereas aerobic growth was observed from ph 5.4. growth rates during aerobiosis were similar at p ... | 2016 | 27375252 |
| effect of endophytic bacillus cereus erbp inoculation into non-native host: potentials and challenges for airborne formaldehyde removal. | phytoremediation could be a cost-effective, environmentally friendly approach for the treatment of indoor air. however, some drawbacks still dispute the expediency of phytotechnology. our objectives were to investigate the competency of plant growth-promoting (pgp) endophytic bacillus cereus erbp (endophyte root blue pea), isolated from the root of clitoria ternatea, to colonize and stabilize within zamioculcas zamiifolia and euphorbia milii as non-native hosts without causing any disease or str ... | 2016 | 27362296 |
| genetic and functional analyses of krs, a locus encoding kurstakin, a lipopeptide produced by bacillus thuringiensis. | bacteria of the bacillus genus are able to synthesize several families of lipopeptides. these small molecules are the product of non-ribosomal peptide synthetases. in 2000, it was found that bacillus thuringiensis, an entomopathogenic bacterium of the bacillus cereus group, produced a previously unknown lipopeptide: kurstakin. genomic analyses reveal that the krs locus, encoding the kurstakin synthetases, is specific to the b. cereus group, but is unevenly distributed within this group. previous ... | 2016 | 27353188 |
| modulation of phytoremediation and plant growth by the treatment with pgpr, ag nanoparticle and untreated municipal wastewater. | the present attempt was made to determine the effects of untreated municipal wastewater (mw) on growth and physiology of maize and to evaluate the role of ag nanoparticle and plant-growth-promoting rhizobacteria (pgpr) when interacting with mw used for irrigation. it was used for the isolation of pgpr. the isolates were identified and characterized based on the colony morphology, c/n source utilization pattern using miniaturized identification system (qts 24), catalase (cat) and oxidase tests, a ... | 2016 | 27348506 |
| photobiologic-mediated fabrication of silver nanoparticles with antibacterial activity. | we present the simple, eco-friendly synthesis of silver nanoparticles (agnps) using sunlight or green, red, blue, or white led light together with dryopteris crassirhizoma rhizome extract (dcre) as the reducing and capping agent. the preliminary indication of agnp production was a color change from yellowish green to brown after light exposure in the presence of dcre. optimization of parameters such as ph, inoculum dose, and metal ion concentration played an important role in achieving nanoparti ... | 2016 | 27348063 |
| chemical profile, antioxidant and antibacterial activities of achillea moschata wulfen, an endemic species from the alps. | aerial parts of achillea moschata wulfen (asteraceae) growing wild in the italian rhaetian alps were investigated to describe, for the first time, their phenolic content, as well as to characterize the essential oil. inspection of the metabolic profile combining hplc-dad and esi-ms/ms data showed that the methanol extract contained glycosylated flavonoids with luteolin and apigenin as the main aglycones. among them, the major compound was 7-o-glucosyl apigenin. caffeoyl derivates were other phen ... | 2016 | 27347915 |
| identification of pyridine synthase recognition sequences allows a modular solid-phase route to thiopeptide variants. | thiopeptides are structurally complex, bioactive natural products derived from ribosomally synthesized and post-translationally modified peptides. a remarkable set of enzymes were recently revealed to catalyze the formation of the core trithiazolylpyridine of thiopeptides via a formal [4 + 2] cycloaddition. these pyridine synthases typically act late in thiopeptide biosynthesis to affect macrocyclization and cleavage of the n-terminal leader peptide, making them potentially useful biocatalysts f ... | 2016 | 27575591 |
| teaching neuroimages: diffuse cerebral vasospasm and multiple intracranial abscesses from bacillus cereus. | 2016 | 27572436 | |
| a catalytic role for c-h/π interactions in base excision repair by bacillus cereus dna glycosylase alkd. | dna glycosylases protect genomic integrity by locating and excising aberrant nucleobases. substrate recognition and excision usually take place in an extrahelical conformation, which is often stabilized by π-stacking interactions between the lesion nucleobase and aromatic side chains in the glycosylase active site. bacillus cereus alkd is the only dna glycosylase known to catalyze base excision without extruding the damaged nucleotide from the dna helix. instead of contacting the nucleobase itse ... | 2016 | 27571247 |
| activation of the class ib ribonucleotide reductase by a flavodoxin reductase in bacillus cereus. | to reduce ribonucleotides to deoxyribonucleotides, the manganese-bound form of class ib ribonucleotide reductase (rnr) must be activated via a pathway that involves redox protein(s). the reduced flavoprotein nrdi is an important protein in this pathway, as it reduces dioxygen to superoxide. superoxide then reacts with the rnr mn(ii)2 site to generate a tyrosyl radical that is required for catalysis. a native nrdi reductase has not yet been identified. we herein demonstrate through kinetic and sp ... | 2016 | 27559930 |
| molecular phylogeny of heterotrophic nitrifiers and aerobic denitrifiers and their potential role in ammonium removal. | to investigate the physiology and taxonomic composition of the key players of nitrification and denitrification processes in paddy fields, culture dependent and independent studies have been carried out. a total of 28 bacterial strains have been screened in which six were capable of reducing nitrate and nitrite as well as having significant ammonium removal potential. 16s rrna-pcr-dgge-based molecular typing of enriched batch culture was done with time duration to explore and identify dominant a ... | 2016 | 27037833 |
| influence of season and type of restaurants on sashimi microbiota. | in recent years, an increase in the consumption of japanese food in european countries has been verified, including in portugal. these specialities made with raw fish, typical japanese meals, have been prepared in typical and on non-typical restaurants, and represent a challenge to risk analysis on haccp plans. the aim of this study was to evaluate the influence of the type of restaurant, season and type of fish used on sashimi microbiota. | 2016 | 26911490 |
| occurrence and antibacterial susceptibility pattern of bacterial pathogens isolated from diarrheal patients in pakistan. | to determine the occurrence of bacterial pathogens responsible for diarrhea and to engender information regarding the effectiveness of commonly used antibiotic against diarrhea. | 2016 | 26905349 |
| systematic evaluation of cs-rosetta for membrane protein structure prediction with sparse noe restraints. | we critically test and validate the cs-rosetta methodology for de novo structure prediction of α-helical membrane proteins (mps) from nmr data, such as chemical shifts and noe distance restraints. by systematically reducing the number and types of noe restraints, we focus on determining the regime in which mp structures can be reliably predicted and pinpoint the boundaries of the approach. five mps of known structure were used as test systems, phototaxis sensory rhodopsin ii (psrii), a subdomain ... | 2016 | 27936510 |
| bacillus cereus-induced food-borne outbreaks in france, 2007 to 2014: epidemiology and genetic characterisation. | the aim of this study was to identify and characterise bacillus cereus from a unique national collection of 564 strains associated with 140 strong-evidence food-borne outbreaks (fbos) occurring in france during 2007 to 2014. starchy food and vegetables were the most frequent food vehicles identified; 747 of 911 human cases occurred in institutional catering contexts. incubation period was significantly shorter for emetic strains compared with diarrhoeal strains a sub-panel of 149 strains strictl ... | 2016 | 27934583 |