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a novel enzyme-based antimicrobial system comprising iodide and a multicopper oxidase isolated from alphaproteobacterium strain q-1.alphaproteobacterium strain q-1 produces an extracellular multicopper oxidase (iox), which catalyzes iodide (i-) oxidation to form molecular iodine (i2). in this study, the antimicrobial activity of the iox/iodide system was determined. both gram-positive and gram-negative bacteria tested were killed completely within 5 min by 50 mu ml(-1) of iox and 10 mm iodide. the sporicidal activity of the system was also tested and compared with a common iodophor, povidone-iodine (pvp-i). iox (300 mu ml(-1 ...201526254787
genome sequence of bacillus endophyticus and analysis of its companion mechanism in the ketogulonigenium vulgare-bacillus strain consortium.bacillus strains have been widely used as the companion strain of ketogulonigenium vulgare in the process of vitamin c fermentation. different bacillus strains generate different effects on the growth of k. vulgare and ultimately influence the productivity. first, we identified that bacillus endophyticus hbe603 was an appropriate strain to cooperate with k. vulgare and the product conversion rate exceeded 90% in industrial vitamin c fermentation. here, we report the genome sequencing of the b. e ...201526248285
quantitative prevalence and toxin gene profile of bacillus cereus from ready-to-eat vegetables in south korea.ready-to-eat (rte) foods such as prepared vegetables are becoming an increasingly popular food choice. since rte vegetables are not commonly sterilized by heat treatment, contamination with foodborne pathogens such as bacillus cereus (b. cereus) is a major concern. the objective of this study was to assess the quantitative prevalence and toxin gene profiles of b. cereus strains isolated from rte vegetables. we found that 70 of the 145 (48%) tested retail vegetable salad and sprout samples were p ...201526317539
food sensing: aptamer-based trapping of bacillus cereus spores with specific detection via real time pcr in milk.aerobic spores pose serious problems for both food product manufacturers and consumers. milk is particularly at risk and thus an important issue of preventive consumer protection and quality assurance. the spore-former bacillus cereus is a food poisoning gram-positive pathogen which mainly produces two different types of toxins, the diarrhea inducing and the emetic toxins. reliable and rapid analytical assays for the detection of b. cereus spores are required, which could be achieved by combinin ...201526306797
analysis of the bacillus cereus spoiis antitoxin-toxin system reveals its three-component nature.programmed cell death in bacteria is generally associated with two-component toxin-antitoxin systems. the spoiis toxin-antitoxin system, consisting of a membrane-bound spoiisa toxin and a small, cytosolic antitoxin spoiisb, was originally identified in bacillus subtilis. in this work we describe the bacillus cereus spoiis system which is a three-component system, harboring an additional gene spoiisc. its protein product serves as an antitoxin, and similarly as spoiisb, is able to bind spoiisa an ...201526300872
endophthalmitis caused by bacillus cereus: a devastating ophthalmological emergency. 201526493084
food-bacteria interplay: pathometabolism of emetic bacillus cereus.bacillus cereus is a gram-positive endospore forming bacterium known for its wide spectrum of phenotypic traits, enabling it to occupy diverse ecological niches. although the population structure of b. cereus is highly dynamic and rather panmictic, production of the emetic b. cereus toxin cereulide is restricted to strains with specific genotypic traits, associated with distinct environmental habitats. cereulide is an ionophoric dodecadepsipeptide that is produced non-ribosomally by an enzyme co ...201526236290
complete genome sequence analysis and identification of putative metallo-beta-lactamase and spoiiie homologs in bacillus cereus group phage bcp8-2, a new member of the proposed bastille-like group.bacillus cereus group-specific bacteriophage bcp8-2 exhibits a broad lysis spectrum among food and human isolates (330/364) of b. cereus while not infecting b. subtilis (50) or b. licheniformis (12) strains. its genome is 159,071 bp long with 220 open reading frames, including genes for putative methyltransferases, metallo-beta-lactamase, and a sporulation-related spoiiie homolog, as wells as 18 trnas. comparative genome analysis showed that bcp8-2 is related to the recently proposed bastille-li ...201526234184
antibacterial effects of cissus welwitschii and triumfetta welwitschii extracts against escherichia coli and bacillus cereus.antibiotic resistance has increased sharply, while the pace for the development of new antimicrobials has slowed down. plants provide an alternative source for new drugs. this study aimed to screen extracts from cissus welwitschii and triumfetta welwitschii for antibacterial activity against escherichia coli and bacillus cereus. the tests conducted included a susceptibility determination test, analysis of the effect of t. welwitschii on cell wall integrity, and transport across the membrane. it ...201526904744
infective endocarditis due to bacillus cereus in a pregnant female: a case report and literature review.incidence of infective endocarditis during pregnancy is around 0.006% with high maternal and fetal mortality. bacillus cereus is an extremely rare cause for endocarditis in intravenous drug abusers (ivda) or those with valvular disease or devices such as pacemakers. we report a case of b. cereus endocarditis, which, to the best of our knowledge, has never been reported in pregnancy. a 30-year-old, 25-week pregnant female presented with right shoulder pain, swelling and erythema on the lateral as ...201526793477
cody regulates the activity of the virulence quorum sensor plcr by controlling the import of the signaling peptide papr in bacillus thuringiensis.in gram-positive bacteria, cell-cell communication mainly relies on cytoplasmic sensors of the rnpp family. activity of these regulators depends on their binding to secreted signaling peptides that are imported into the cell. these quorum sensing regulators control important biological functions in bacteria of the bacillus cereus group, such as virulence and necrotrophism. the rnpp quorum sensor plcr, in complex with its cognate signaling peptide papr, is the main regulator of virulence in b. ce ...201526779156
antimicrobial effect of nisin against bacillus cereus in beef jerky during storage.the microbial distribution of raw materials and beef jerky, and the effect of nisin on the growth of bacillus cereus inoculated in beef jerky during storage, were studied. five strains of pathogenic b. cereus were detected in beef jerky, and identified with 99.8% agreement using api chb 50 kit. to evaluate the effect of nisin, beef jerky was inoculated with approximately 3 log cfu/g of b. cereus mixed culture and nisin (100 iu/g and 500 iu/g). during the storage of beef jerky without nisin, the ...201526761838
evaluation of anti-bacterial effects of some novel thiazole and imidazole derivatives against some pathogenic bacteria.bacterial resistance to antibiotics has motivated the researchers to evaluate the novel anti-bacterial compounds such as some thiazole and imidazole derivatives. thereby, in this work, we investigated the anti-bacterial effects of one new thiazole and two new imidazole derivatives on bacillus cereus, listeria monocytogenes, escherichia coli, salmonella typhimurium, proteus mirabilis and shigella dysenteriae.201526719785
adaptive responses of bacillus cereus atcc14579 cells upon exposure to acid conditions involve atpase activity to maintain their internal ph. 201526769448
synthesis and properties of cellulose-functionalized poss-sio2/tio2 hybrid composites.the mechanical, thermal, optical, electrical and morphological properties of cellulose, an excellent natural biomaterial, can be improved by organic-inorganic hybrid composite methods. based on the pristine properties of cellulose, the preparation of cellulose-metal oxide hybrid nanocomposites using a dispersion process of nanoparticles into the cellulose host matrix by traditional methods, has limitations. recently, the functionalized cellulose-polymer-based materials were considered to be an i ...201526726461
next-generation whole-genome sequencing of eight strains of bacillus cereus, isolated from food.bacillus cereus can contaminate food and cause emetic and diarrheal foodborne illness. here, we report whole-genome sequences of eight strains of b. cereus, isolated from different food sources.201526679589
subset selection of high-depth next generation sequencing reads for de novo genome assembly using mapreduce framework.recent progress in next-generation sequencing technology has afforded several improvements such as ultra-high throughput at low cost, very high read quality, and substantially increased sequencing depth. state-of-the-art high-throughput sequencers, such as the illumina miseq system, can generate ~15 gbp sequencing data per run, with >80% bases above q30 and a sequencing depth of up to several 1000x for small genomes. illumina hiseq 2500 is capable of generating up to 1 tbp per run, with >80% bas ...201526678408
structure elucidation, antimicrobial and cytotoxic activities of a halimane isolated from vellozia kolbekii alves (velloziaceae).a new halimane diterpene was isolated from vellozia kolbekii alves (velloziaceae) and identified as (5r,8r,9s,13r)-halim-1,10-ene-15,16-diol (1). it showed cytotoxicity against three human cancer cell lines, sf-295 (glioblastoma), mda-mb-435 (melanoma), and hct-8 (colon adenocarcinoma). in the mechanism of cytotoxic action, halimane 1 interferes in two major phases of the cell cycle: in s phase, in which dna synthesis occurs and where it is very sensitive to damage, and g2m phase which is the ph ...201526663842
genome sequence of a chromium-reducing strain, bacillus cereus s612.we report here the genome sequence of an effective chromium-reducing bacterium, bacillus cereus strain s612. the size of the draft genome sequence is approximately 5.4 mb, with a g+c content of 35%, and it is predicted to contain 5,450 protein-coding genes.201526659672
phytofabrication of bioinduced silver nanoparticles for biomedical applications.synthesis of nanomaterials holds infinite possibilities as nanotechnology is revolutionizing the field of medicine by its myriad applications. green synthesis of nanoparticles has become the need of the hour because of its eco-friendly, nontoxic, and economic nature. in this study, leaf extract of rosa damascena was used as a bioreductant to reduce silver nitrate, leading to synthesis of silver nanoparticles (agnps) in a single step, without the use of any additional reducing or capping agents. ...201526648715
the endospore-forming pathogen bacillus cereus exploits a small colony variant-based diversification strategy in response to aminoglycoside exposure.bacillus cereus is among the microorganisms most often isolated from cases of food spoilage and causes gastrointestinal diseases as well as nongastrointestinal infections elicited by the emetic toxin cereulide, enterotoxins, and a panel of tissue-destructive virulence factors. this opportunistic pathogen is increasingly associated with rapidly fatal clinical infections especially linked to neonates and immunocompromised individuals. fatality results from either the misdiagnosis of b. cereus as a ...201526646008
root associated bacillus sp. improves growth, yield and zinc translocation for basmati rice (oryza sativa) varieties.plant associated rhizobacteria prevailing in different agro-ecosystems exhibit multiple traits which could be utilized in various aspect of sustainable agriculture. two hundred thirty four isolates were obtained from the roots of basmati-385 and basmati super rice varieties growing in clay loam and saline soil at different locations of punjab (pakistan). out of 234 isolates, 27 were able to solubilize zinc (zn) from different zn ores like zinc phosphate [zn3 (po4)2], zinc carbonate (znco3) and z ...201526635754
silver nanoparticles cause complications in pregnant mice.silver nanoparticles (agnps) have attracted much interest and have been used for antibacterial, antifungal, anticancer, and antiangiogenic applications because of their unique properties. the increased usage of agnps leads to a potential hazard to human health. however, the potential effects of agnps on animal models are not clear. this study was designed to investigate the potential impact of agnps on pregnant mice.201526622177
application of a rapid knowledge synthesis and transfer approach to assess the microbial safety of low-moisture foods.low-moisture foods (lmf) are increasingly implicated in outbreaks of foodborne illness, resulting in a significant public health burden. to inform the development of a new codex alimentarius code of hygienic practice for lmf, we applied a rapid knowledge synthesis and transfer approach to review global research on the burden of illness, prevalence, and interventions to control nine selected microbial hazards in eight categories of lmf. knowledge synthesis methods included an integrated scoping r ...201526613924
changes in the microbial composition of microbrewed beer during the process in the actual manufacturing line.this study investigated changes in the microbial composition of microbrewed beer during the manufacturing processes and identified potential microbial hazards, effective critical quality control points, and potential contamination routes. comprehensive quantitative (aerobic plate count, lactic acid bacteria, fungi, acetic acid bacteria, coliforms, and bacillus cereus) and qualitative (escherichia coli and eight foodborne pathogens) microbiological analyses were performed using samples of raw mat ...201526613919
the mutation glu151asp in the b-component of the bacillus cereus non-hemolytic enterotoxin (nhe) leads to a diverging reactivity in antibody-based detection systems.the ability of bacillus cereus to cause foodborne toxicoinfections leads to increasing concerns regarding consumer protection. for the diarrhea-associated enterotoxins, the assessment of the non-hemolytic enterotoxin b (nheb) titer determined by a sandwich enzyme immunoassay (eia) correlates best with in vitro cytotoxicity. in general, the regulation of enterotoxin expression of b. cereus is a coordinately-regulated process influenced by environmental, and probably also by host factors. as long ...201526569304
design and synthesis of new 2-aryl-4,5-dihydro-thiazole analogues: in vitro antibacterial activities and preliminary mechanism of action.sixty 2-aryl-4,5-dihydrothiazoles were designed and synthesized in yields ranging from 64% to 89% from cysteine and substituted-benzonitriles via a novel metal- and catalyst-free method. the structures of the title compounds were confirmed mainly by nmr spectral data analysis. antibacterial activity assays showed that the compounds (s)-2-(2'-hydroxyphenyl)-4-hydroxy-methyl- 4,5-dihydrothiazole (7h) and (r)-2-(2'-hydroxyphenyl)-4-hydroxymethyl-4,5-dihydro-thiazole (7h') exhibited significant inhi ...201526569197
complete genome sequence of bacillus cereus forc_005, a food-borne pathogen from the soy sauce braised fish-cake with quail-egg.due to abundant contamination in various foods, the pathogenesis of bacillus cereus has been widely studied in physiological and molecular level. b. cereus forc_005 was isolated from a korean side dish, soy sauce braised fish-cake with quail-egg in south korea. while 21 complete genome sequences of b. cereus has been announced to date, this strain was completely sequenced, analyzed, and compared with other complete genome sequences of b. cereus to elucidate the distinct pathogenic features of a ...201526566422
antibacterial action against food-borne microorganisms and antioxidant activity of carvacrol-rich oil from lippia origanoides kunth.lippia origanoides kunth from northeast brazil is a plant of pleasant odor used by local people as a food seasoning in substitution the oregano where its carvacrol-rich oil has showed significant antimicrobial activity against human pathogens.201526552874
bacillus cereus adhesion to simulated intestinal mucus is determined by its growth on mucin, rather than intestinal environmental parameters.adhesion of pathogenic bacteria to intestinal mucus, the protective layer of the gastrointestinal epithelium, is often considered a virulence factor. the ability of food-poisoning bacillus cereus strains to attach to mucus and the factors affecting this interaction have not yet been investigated. therefore, the role of adhesion in pathogenesis of b. cereus still remains unknown. in the present study, an in vitro assay based on mucin agar was used to simulate adhesion of b. cereus to mucus. bacte ...201526545170
[screening of molluscacidal microorganisms against oncomelania hupensis and their effect].fifteen soil samples were collected from oncomelania hupensis culture pond in miluo schistosomiasis control and prevention base, hunan province. four strains of bacteria were identified to have molluscacidal effects, numbered as b8, b27, b36 and b59. compared with the fermentation broth groups and bacteria suspension groups, the fermentation supernatant groups of the four strains showed the strongest molluscacidal effect. the fermentation supernatant of b59 strain showed the best molluscacidal e ...201526541047
comparison of primary models to predict microbial growth by the plate count and absorbance methods.the selection of a primary model to describe microbial growth in predictive food microbiology often appears to be subjective. the objective of this research was to check the performance of different mathematical models in predicting growth parameters, both by absorbance and plate count methods. for this purpose, growth curves of three different microorganisms (bacillus cereus, listeria monocytogenes, and escherichia coli) grown under the same conditions, but with different initial concentrations ...201526539483
identification of novel tylosin analogues generated by a wbla disruption mutant of streptomyces ansochromogenes.streptomyces, as the main source of antibiotics, has been intensively exploited for discovering new drug candidates to combat the evolving pathogens. disruption of wbla, an actinobacteria-specific gene controlling major developmental transition, can cause the alteration of phenotype and morphology in many species of streptomyces. one wbla homologue was found in streptomyces ansochromogenes 7100 by using the basic local alignment search tool. it is interesting to identify whether novel secondary ...201526525981
heavy metal detoxification by different bacillus species isolated from solar salterns.the biosorption mechanism is an alternative for chemical precipitation and ultrafiltration which have been employed to treat heavy metal contamination with a limited success. in the present study, three species of bacillus which were isolated from solar salterns were screened for their detoxification potential of the heavy metals, lead, chromium, and copper, by biosorption. biosorption potential of each isolate was determined by atomic absorption spectroscopy (aas), inductively coupled plasma-op ...201526525498
erratum for erill and caruso, complete genome sequence of bacillus cereus group phage tsarbomba. 201526586903
fibrinolytic enzyme production by newly isolated bacillus cereus srm-001 with enhanced in-vitro blood clot lysis potential.the discovery of plasmin-like microbial fibrinolytic enzymes having high specificity and negligible side effects is crucial for thrombolytic therapy. herein, we report one such extra-cellular fibrinolytic enzyme producing bacillus cereus srm-001 isolated from the blood-laden soil of a chicken dump yard. the potency of the enzyme was established with fibrin plate assay and in-vitro blood clot lysis assay. the shake-flask operating parameters and media composition were optimized for maximizing the ...201526582284
phenalenone derivatives and the unusual tricyclic sesterterpene acid from the marine fungus lophiostoma bipolare bcc25910.ten compounds including nine phenalenone derivatives (five bipolarides and four bipolarols) and a sesterterpene acid (bipolarenic acid), were isolated from a marine isolated of the fungus lophiostoma bipolare (bcc25910), along with the known compounds, (-)-scleroderolide, (-)-sclerodin, and oxasetin. chemical structures were elucidated based on nmr spectroscopic data and hresims analysis. in addition, the absolute configurations of the phenalenones were resolved using specific rotations and chem ...201526582262
molecular characterization and biological effects of a c-type lectin-like receptor in large yellow croaker (larimichthys crocea).the c-type lectin-like receptors (ctlrs) play important roles in innate immunity as one type of pattern recognition receptors. here, we cloned and characterized a c-type lectin-like receptor (lycctlr) from large yellow croaker larimichthys crocea. the full-length cdna of lycctlr is 880 nucleotides long, encoding a protein of 215 amino acids. the deduced lycctlr contains a c-terminal c-type lectin-like domain (ctld), an n-terminal cytoplasmic tail, and a transmembrane region. the ctld of lycctlr ...201526690423
cost-effective-substrates for production of poly-β-hydroxybutyrate by a newly isolated bacillus cereus ps-10.poly-β-hydroxybutyrate (phb) may serve as one of the imperative substitutes for petroleum derived plastics because of their close functional analogy and biodegradation quality. in the present study, phb producing ability of bacterial isolates was examined on low-cost agro industrial residues. isolate ps-10 from domestic waste landfills, identified as bacillus cereus ps-10 produced and accumulated appreciable amount of phb. bacillus cereus ps-10 was capable of using a wide variety of agro-based r ...201526688964
the dna glycosylase alkd uses a non-base-flipping mechanism to excise bulky lesions.threats to genomic integrity arising from dna damage are mitigated by dna glycosylases, which initiate the base excision repair pathway by locating and excising aberrant nucleobases. how these enzymes find small modifications within the genome is a current area of intensive research. a hallmark of these and other dna repair enzymes is their use of base flipping to sequester modified nucleotides from the dna helix and into an active site pocket. consequently, base flipping is generally regarded a ...201526524531
high-level production of bacillus cereus phospholipase c in corynebacterium glutamicum.enzymatic oil degumming (removal of phospholipids) using phospholipase c (plc) is a well-established and environmentally friendly process for vegetable oil refining. in this work, we report the production of recombinant bacillus cereus plc in corynebacterium glutamicum atcc 13869 in a high cell density fermentation process and its performance in soybean oil degumming. a final concentration of 5.5g/l of the recombinant enzyme was achieved when the respective gene was expressed from the tac promot ...201526519562
microbiological quality of raw dried pasta from the german market, with special emphasis on cronobacter species.the microbiological quality of 132 dried pasta products available on the german market, originating from 11 different countries, was studied. sample materials included soft or durum wheat products, some of which produced with other ingredients such as eggs, spices, or vegetables. parameters included hygiene indicators (aerobic plate count, mold count, the presence of enterobacteriaceae) and pathogenic/toxinogenic bacterial species (salmonella spp., staphylococcus aureus, presumptive bacillus cer ...201526509868
the c-terminal zwitterionic sequence of cotb1 is essential for biosilicification of the bacillus cereus spore coat.silica is deposited in and around the spore coat layer of bacillus cereus, and enhances the spore's acid resistance. several peptides and proteins, including diatom silaffin and silacidin peptides, are involved in eukaryotic silica biomineralization (biosilicification). homologous sequence search revealed a silacidin-like sequence in the c-terminal region of cotb1, a spore coat protein of b. cereus. the negatively charged silacidin-like sequence is followed by a positively charged arginine-rich ...201526503850
proteomics identifies bacillus cereus entd as a pivotal protein for the production of numerous virulence factors.bacillus cereus is a gram-positive pathogen that causes a wide variety of diseases in humans. it secretes into the extracellular milieu proteins that may contribute directly or indirectly to its virulence. entd is a novel exoprotein identified by proteogenomics of b. cereus atcc 14579. we constructed a δentd mutant and analyzed the impact of entd disruption on the cellular proteome and exoproteome isolated from early, late, and stationary-phase cultures. we identified 308 and 79 proteins regulat ...201526500610
antioxidant, antibacterial activity, and phytochemical characterization of melaleuca cajuputi extract.the threat posed by drug-resistant pathogens has resulted in the increasing momentum in research and development for effective alternative medications. the antioxidant and antibacterial properties of phytochemical extracts makes them attractive alternative complementary medicines. therefore, this study evaluated the phytochemical constituents of melaleuca cajuputi flower and leaf (gf and gl, respectively) extracts and their antioxidant and antibacterial activities.201526497742
bacillus cereus nvh 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation.adhesion to the intestinal epithelium could constitute an essential mechanism of bacillus cereus pathogenesis. however, the enterocytes are protected by mucus, a secretion composed mainly of mucin glycoproteins. these may serve as nutrients and sites of adhesion for intestinal bacteria. in this study, the food poisoning bacterium b. cereus nvh 0500/00 was exposed in vitro to gastrointestinal hurdles prior to evaluation of its attachment to mucin microcosms and its ability to produce nonhemolytic ...201526497468
sporulation temperature reveals a requirement for cote in the assembly of both the coat and exosporium layers of bacillus cereus spores.the bacillus cereus spore surface layers consist of a coat surrounded by an exosporium. we investigated the interplay between the sporulation temperature and the cote morphogenetic protein in the assembly of the surface layers of b. cereus atcc 14579 spores and on the resulting spore properties. the cote deletion affects the coat and exosporium composition of the spores formed both at the suboptimal temperature of 20°c and at the optimal growth temperature of 37°c. transmission electron microsco ...201526497467
evaluation of bacterial contamination in raw milk, ultra-high temperature milk and infant formula using single molecule, real-time sequencing technology.the pacific biosciences (menlo park, ca) single molecule, real-time sequencing technology (smrt) was reported to have some advantages in analyzing the bacterial profile of environmental samples. in this study, the presence of bacterial contaminants in raw milk, uht milk, and infant formula was determined by smrt sequencing of the full length 16s rrna gene. the bacterial profiles obtained at different taxonomic levels revealed clear differences in bacterial community structure across the 16 analy ...201526476945
synthesis, evaluation and molecular docking studies of amino acid derived n-glycoconjugates as antibacterial agents.six amino acid derived n-glycoconjugates of d-glucose were synthesized, characterized and tested for antibacterial activity against g(+)ve (bacillus cereus) as well as g(-)ve (escherichia coli and klebsiella pneumoniae) bacterial strains. all the tested compounds exhibited moderate to good antibacterial activity against these bacterial strains. the results were compared with the antibacterial activity of standard drug chloramphenicol, where results of a5 (tryptophan derived glycoconjugates) agai ...201526476390
purification and characterization of bacteriocin produced by weissella confusa a3 of dairy origin.a dramatic increase in bacterial resistance towards currently available antibiotics has raised worldwide concerns for public health. therefore, antimicrobial peptides (amps) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. the present investigation focusses on the isolation and purification of a novel bacteriocin from an indigenous sample of cow milk and it's mode of action. the bacteriocin was isolated from weissella confusa a3 that was isolated fr ...201526474074
enteric bacteria isolated from diarrheal patients in korea in 2014.the aim of this study was to characterize the pathogens responsible for causing diarrhea according to season, region of isolation, patient age, and sex as well as to provide useful data for the prevention of diarrheal disease.201526473090
genome sequences of two bacillus cereus group bacteriophages, eyuki and avesobmore.the genomes of two double-stranded dna (dsdna) bacteriophages isolated on bacillus thuringiensis show similarity to previously sequenced phages and provide evidence of the mosaicism of phage genomes.201526472840
complete genome sequence of bacillus cereus group phage tsarbomba.the bacillus cereus group bacteriophage tsarbomba, a double-stranded dna myoviridae, was isolated from soil collected in saratov, russia. tsarbomba was found to be similar to bacillus phages bcp78 and bcu4, and to have a wide host range among bacillus cereus group species.201526472830
total phenolic and flavonoid content and antibacterial activity of punica granatum l. var. pleniflora flowers (golnar) against bacterial strains causing foodborne diseases.flowers of punica granatum l. (punicaceae) var. pleniflora, known as "golnar" in iranian traditional medicine have been used for the prevention and treatment of foodborne diseases. in this study, antibacterial activities of ethanol extract of golnar and its fractions were scientifically evaluated against bacteria causing foodborne diseases including staphylococcus aureus, bacillus cereus, listeria monocytogenes, escherichia coli, shigella dysantriae, and salmonella typhi. the total phenolic and ...201526470879
differential oxidative stress responses in castor semilooper, achaea janata.balance between reactive oxygen species (ros) and the antioxidant (ao) defense mechanisms is vital for organism survival. insects serve as an ideal model to elucidate oxidative stress responses as they are prone to different kinds of stress during their life cycle. the present study demonstrates the modulation of ao enzyme gene expression in the insect pest, achaea janata (castor semilooper), when subjected to different oxidative stress stimuli. antioxidant enzymes' (catalase (cat), superoxide d ...201526455997
novel diesel-oil-degrading bacteria and fungi from the ecuadorian amazon rainforest.isolating new diesel-oil-degrading microorganisms from crude-oil contaminated sites and evaluating their degradation capacities are vitally important in the remediation of oil-polluted environments and crude-oil exploitation. in this research, new hydrocarbon-degrading bacteria and fungi were isolated from the crude-oil contaminated soil of the oil-fields in the amazon rainforest of north-east ecuador by using a soil enrichment technique. degradation analysis was tracked by gas chromatography an ...201526442498
[noma/cancrum oris: a neglected disease].noma is an aggressive orofacial gangrenous pathology that damages hard and soft tissues of the mouth and the face. throughout the centuries it has been present around the globe, but nowadays it has practically disappeared from developed countries and mainly affects children from the most disadvantaged places, especially in africa. noma disease is a multifactorial process; malnutrition, debilitating diseases (bacterial or viral systemic diseases, hiv-associated immunosuppression, etc.) and intrao ...201526437752
short communication: elucidation of bacterial community structure on thin-spined porcupine (chaetomys subspinosus) spines by denaturing.thin-spined porcupines (chaetomys subspinosus) are threatened with extinction and are categorized as vulnerable. this is because of alteration to and loss of their habitat and possible hunting activities in their distribution area. their spines constitute one of their defense mechanisms, which can be fomites for pathogens to humans. however, little is known about such pathogens. the present study aimed to detect bacteria on spines of c. subspinosus, from the una biological reserve, south of bahi ...201526436511
comparison of antibacterial activity of ag nanoparticles synthesized from leaf extract of parthenium hystrophorus l in aqueous media and gentamicin sulphate: in-vitro.monodisperse silver (ag) nanoparticles were synthesized by using parthenium hystrophorus l leaf extract in aqueous media. the synthesized nanoparticles were characterized by using uv-vis spectrophotometer, x-ray diffracto-meter (xrd), transmission electron microscope (tem), and dynamics light scattering (dls). size-dependent antibacterial activities of ag nanoparticles were tested against gram negative pseudomonas aeruginosa and gram positive staphylococcus aureus. ag nanoparticles having 20 ± 2 ...201524111829
mercuric reductase activity of multiple heavy metal-resistant lysinibacillus sphaericus g1.a culture was isolated from an industrial mercuric salt-contaminated soil, which could tolerate cd, co, zn, cr, and hg up to 190, 525, 350, 935, and 370 μm, respectively. the isolate was identified as lysinibacillus sphaericus by 16s rrna gene sequencing. it bioaccumulated cd, co, and zn, and reductively detoxified cr and hg. chromate reductase and mercuric reductase (mera) activities in the cell extract were 2.4 and 0.13 units mg(-1) protein, respectively. the study also describes designing of ...201524132860
enhancement of mechanical properties, microstructure, and antimicrobial activities of zein films cross-linked using succinic anhydride, eugenol, and citric acid.zein constitutes about half of the endosperm proteins in corn. recently, attempts have been made to utilize zein for food coatings and biodegradable materials, which require better physical properties, using chemical modification of zein. in this study, zein proteins were modified using citric acid, succinic anhydride, and eugenol as natural cross-linking agents in the wet state. the cross-linkers were added either separately or combined in increment concentrations (0.1, 0.2, 0.3, and 0.4%). the ...201525036665
functional characterization and phylogenetic analysis of acquired and intrinsic macrolide phosphotransferases in the bacillus cereus group.the bacillus cereus group is composed of gram-positive spore-forming bacteria of clinical and ecological importance. more than 200 b. cereus group isolates have been sequenced. however, there are few reports of b. cereus group antibiotic resistance genes. this study identified two functional classes of macrolide phosphotransferases (mphs) in the b. cereus group. cluster a mphs inactivate 14- and 15-membered macrolides while cluster b mphs inactivate 14-, 15- and 16-membered compounds. the genomi ...201525059531
antimicrobial and allelopathic metabolites produced by penicillium brasilianum.six known compounds, isoroquefortine c (1), griseofulvin (2), ergosterol peroxide (3), 3β-hydroxy-(22e,24r)-ergosta-5,8,22-trien-7-one (4), cerevisterol (5) and (22e,24r)-6β-methoxyergosta-7,22-diene-3β,5α-diol (6), were produced by the fungus penicillium brasilianum, and their structures were elucidated by spectroscopic methods. this is the first report on isoroquefortine c as naturally occurring compound. their bioactivities against five phytopathogenic fungi (gibeberalla saubinetti, fusarium ...201525103127
is atomic rearrangement of type iv pha synthases responsible for increased pha production?type iv pha synthase is a key enzyme responsible for catalyzing the formation of non-toxic, biocompatible, and biodegradable short-chain-length polyhydroxyalkanoates (scl-pha) under the growth-limiting conditions in the members of the genus bacillus.201525077376
effect of crude extracts of selected actinomycetes on biofilm formation of a. schindleri, m. aci, and b. cereus.actinomycetes are well known group of gram positive bacteria for their potential to produce antibiotics. this study sought to assess the ability of the selected actinomycetes to control biofilm forming bacteria isolated from different dental plaque samples. on the basis of morphological differences three out of ten different dental plaque bacterial isolates were selected for further study. these isolates were biochemically and genetically characterized and were identified as acinetobacter schinn ...201525138589
structural data and biological properties of almond gum oligosaccharide: application to beef meat preservation.enzymatic hydrolysis of almond gum generates low molecular weight oligosaccharides (oag) with a yield of 33.5%. the generated oligosaccharides were purified and identified. oag analyses show that the most prominent residues were galactose and arabinose with traces of xylose, rhamnose, glucose and mannose. the glycosyl linkage positions were analyzed using gas chromatography-mass spectrometry showing a main chain composed of galactose units [ → 3)-gal-(1 → ] branched mainly with arabinose residue ...201525195541
development of biopolymer nanocomposite for silver nanoparticles and ciprofloxacin controlled release.screening of biopolymeric gel beads containing silver nanoparticles (ag-nps) stabilized in guar gum alkyl amine (ggaa) and ciprofloxacin (cip) was carried out in order to obtain a novel nanocomposite with controlled release profile of both antimicrobians. the selected matrix composed of alginate/high methoxyl pectin (hmp)/ggaa (4:4:1) was able to co-incorporate ag-nps and cip with encapsulation efficiency higher than 70%. sem images revealed good cohesivity and compatibility between the biopolym ...201525256547
bioaugmentation of a sequencing batch biofilm reactor with comamonas testosteroni and bacillus cereus and their impact on reactor bacterial communities.the immobilization of microorganisms is essential for efficient bioaugmentation systems. the performance of bacillus cereus g5 as biofilm-forming bacteria and comamonas testosteroni a3 a 3,5 dinitrobenzoic acid (dnb)-degrading strain] in laboratory-scale sequencing batch biofilm reactors (sbbrs) treating dnb synthetic wastewater has been examined. the microbial diversity in the reactors was also explored. the reactor r3 inoculated with b. cereus g5 and c. testosteroni a3 together not only improv ...201525257599
antibacterial activity and genotypic-phenotypic characteristics of bacteriocin-producing bacillus subtilis kku213: potential as a probiotic strain.the antimicrobial activity and probiotic properties of bacillus subtilis strain kku213, isolated from local soil, were investigated. the cell-free supernatant (cfs) of a kku213 culture containing crude bacteriocins exhibited inhibitory effects on gram-positive bacteria, including bacillus cereus, listeria monocytogenes, micrococcus luteus, and staphylococcus aureus. the antibacterial activity of the cfs precipitated with 40% ammonium sulfate (as) remained even after treatment at 60 and 100 °c, a ...201525440998
probabilistic exposure assessment model to estimate aseptic-uht product failure rate.aseptic-ultra-high-temperature (uht) products are manufactured to be free of microorganisms capable of growing in the food at normal non-refrigerated conditions at which the food is likely to be held during manufacture, distribution and storage. two important phases within the process are widely recognised as critical in controlling microbial contamination: the sterilisation steps and the following aseptic steps. of the microbial hazards, the pathogen spore formers clostridium botulinum and baci ...201525440556
simultaneous efficient removal of high-strength ammonia nitrogen and chemical oxygen demand from landfill leachate by using an extremely high ammonia nitrogen-resistant strain.bioaugmentation is a promising technology for pollutant elimination from stressed environments, and it would provide an efficient way to solve challenges in traditional biotreatment of wastewater with high strength of ammonia nitrogen (nh4(+)-n). a high nh4(+)-n-resistant bacteria strain, identified as bacillus cereus (jlu bc), was domesticated and isolated from the bacteria consortium in landfill leachate. jlu bc could survive in 100 g/l nh4(+)-n environment, which indicated its extremely high ...201525196098
isolation and characterization of a furfural-degrading bacterium bacillus cereus sp. strain ds1.furfural was found to be the main organic pollutant in the wastewater coming from the diosgenin factory. this substance is derived from acidic pentosan in dioscorea zingiberensis and is also found in a variety of agricultural byproducts, including corncobs, oat, wheat bran, and sawdust. it is regarded as a toxicant and an inhibitor to the growth of microorganism in both sewage disposal and biological fermentation. a furfural-degrading strain (ds1) was isolated from activated sludge of wastewater ...201525274411
chemical composition and antimicrobial activity of polish herbhoneys.the present study focuses on samples of polish herbhoneys (hhs), their chemical composition and antimicrobial activity. a gas chromatography-mass spectrometry (gc-ms) method was used to analyse eight samples of herbal honeys and three samples of nectar honeys. their antimicrobial activities were tested on selected gram-positive (bacillus cereus, staphylococcus aureus, staphylococcus schleiferi) and gram-negative (escherichia coli) bacteria, as well as on pathogenic fungi candida albicans. ether ...201525308646
lifesaving liver transplantation for multi-organ failure caused by bacillus cereus food poisoning.bacillus cereus is a spore-forming, gram-positive bacterium that causes food poisoning presenting with either emesis or diarrhea. diarrhea is caused by proteinaceous enterotoxin complexes, mainly hemolysin bl, non-hemolytic enterotoxin (nhe), and cytotoxin k. in contrast, emesis is caused by the ingestion of the depsipeptide toxin cereulide, which is produced in b. cereus contaminated food, particularly in pasta or rice. in general, the illness is mild and self-limiting. however, due to cereulid ...201525323120
an insect gut environment reveals the induction of a new sugar-phosphate sensor system in bacillus cereus.bacteria survive under various conditions by sensing stimuli triggering specific adaptive physiological responses, which are often based on membrane-integrated sensors connected to a cytoplasmic regulator. recent studies reveal that mucus glycans may act as signal molecules for two-component systems involved in intestinal colonization. bacillus cereus, a human and insect opportunistic pathogen was used to identify bacterial factors expressed in an insect gut infection model. the screen revealed ...201524256737
antibacterial, antioxidant and cytotoxic studies of total saponin, alkaloid and sterols contents of decoction of joshanda: identification of components through thin layer chromatography.the current study was aimed to assess antibacterial, antioxidant and cytotoxic activity of total saponin, alkaloid and sterol contents of joshanda decoction followed by its constituent's analysis via thin layer chromatography (tlc). saponins and alkaloids showed prominent antibacterial activity against staphylococcus aureus, bacillus subtilis, bacillus cereus and klebsiella pneumoniae whereas sterols only against s. aureus. saponin and alkaloid contents of 97 and 108 µg/ml, respectively, showed ...201523235996
combined effect of cadmium, lead, and uv rays on bacillus cereus using comet assay and oxidative stress parameters.exposure to environmental chemicals and oxidative stress particularly at low dose levels may produce additive or synergistic interactions not seen in single component exposure. exposure to cadmium, lead, and ultraviolet rays occurs in many occupational settings, such as pigment and battery production, galvanization, and recycling of electric tools. however, little is known about interactions between heavy metals and ultraviolet rays. this study aimed to evaluate the interactions of ultraviolet r ...201523089956
development of intestinal microflora and occurrence of diarrhoea in sucking foals: effects of bacillus cereus var. toyoi supplementation.almost all foals develop transient diarrhoea within the first weeks of life. studies indicated different viral, bacterial, and parasitic causes, such as rotavirus, clostridium perfringens, escherichia coli, and cryptosporidium are discussed. but little is known about the development of intestinal microflora in foals. the present study investigated whether the supplementation with bacillus cereus var. toyoi would modify the developing intestinal microflora and consequently reduce diarrhoea in foa ...201525889817
antimicrobial activity of aqueous, ethanolic and methanolic leaf extracts from acacia spp. and eucalyptus nicholii.in europe, acacia and eucalyptus, originate large amounts of biomass, due to their need by industries and other biological control, that can be used to extract antimicrobial substances.201628480369
in vitro anti-oxidant and anti-microbial potentiality investigation of different fractions of caryota urens leaves.caryota urens is a member of the arecaceae family and a common plant in the southeast asian region. this plant has been reported as an anti-microbial agent in recent years. thus, we aimed to find out the mic (minimum inhibitory concentration) against different pathogenic microorganism.201628536384
[septicaemia of chironomid larvae (diptera: chironomidae) promoted by bacillus cereus and b. thuringiensis].natural factors regulating the population of chironomids were studied. the bacteria bacillus cereus were isolated from chironomids sampled from kuyalnitskii firth after epizooty of chironomus sp., and bacteria bacillus thuringiensis spp. israelensis (bti) were isolated from dead larva of chironomus plumosus sampled in the sea of azov (3-m depth). bti were characterized by high insecticide activity on midges anopheles messeae fall., aedes cireneus mg., and culex pipiens pipiens f. pipiens l.201622988756
chemistry and biological activities of tanacetum chiliophyllum var. oligocephalum extracts.tanacetum chiliophyllum (fisch. & mey.) var. oligocephalum (d.c.) sosn. collected in turkey was subjected to phytochemical and biological evaluations in this study. pure compounds were obtained from ethyl acetate extracts of the stems of the plant material. structures of isolated compounds were determined using spectral methods. seven known flavones, i.e., 5-hydroxy-3',4',6,7-tetramethoxyflavone, eupatilin (6-hydroxyluteolin-6,3',4'-trimethylether), cirsimaritin (scuttellarin-6,7-dimethylether), ...201624645497
effect of different in vitro culture extracts of black pepper (piper nigrum l.) on toxic metabolites-producing strains.in the present study, the effect of different in vitro cultures (callus, in vitro shoots) and commercially available peppercorn extract was investigated for its activity against toxic metabolite-producing strains (escherichia coli, pseudomonas aeroginosa, salmonella typhi, bacillus subtilis, bacillus cereus, staphylococcus aureus, and candida albicans). these in vitro cultures were extracted with ethanol, hexane, and chloroform, and the antipathogenic activity was determined by well-diffusion me ...201624193053
bacillus cereus septicemia in a patient with acute lymphoblastic leukemia: a case report and review of the literature.bacillus cereus is an aerobic gram-positive, spore-forming, rod-shaped bacterium that is responsible for foodborne illnesses. we report on a 15-year-old girl with b-cell acute lymphoblastic leukemia, who fell into a somnolent state after presenting with a 12-hour history of fever, muscle soreness, myalgia in both calves, sore throat, and vomiting. fulminant septicemic syndrome caused by b. cereus was finally identified. the aim of this work is the introduction of b. cereus as a differential diag ...201623927823
transcription factors wrky70 and wrky11 served as regulators in rhizobacterium bacillus cereus ar156-induced systemic resistance to pseudomonas syringae pv. tomato dc3000 in arabidopsis.the activation of both the sa and ja/etsignalling pathways may lead to more efficient general and broad resistance to pst dc3000 by non-pathogenic rhizobacteria. however, the mechanisms that govern this simultaneous activation are unclear. using arabidopsis as a model system, two transcription factors, wrky11 and wrky70, were identified as important regulators involved in induced systemic resistance (isr) triggered by bacillus cereus ar156. the results revealed that ar156 treatment significantly ...201626433201
single-step purification of chitosanases from bacillus cereus using expanded bed chromatography.a chitosanase-producing strain was isolated and identified as bacillus cereus c-01. the purification and characterization of two chitosanases were studied. the purification assay was accomplished by ion exchange expanded-bed chromatography. experiments were carried out in the presence and in the absence of cells through different expansion degree to evaluate the process performance. the adsorption experiments demonstrated that the biomass does not affect substantially the adsorption capacity of ...201626433174
rapid enhanced photocatalytic degradation of dyes using novel n-doped zro2.a novel n-doped zro2 (n-zro2) photocatalyst is synthesized through thermal decomposition of zirconium hydroxide-urea complex and is characterized using various techniques, including xrd, ftir, tga, sem, tem, uv-drs, xps, xanes, and bet. the n-zro2 possesses pure monoclinic structure with high crystallinity. by using the proposed facile route of synthesis, both interstitial and substitutional n doping with high dopant stability can be realized. the optical properties of the catalyst are significa ...201626439860
recent research progress with phospholipase c from bacillus cereus.phospholipase c (plc) catalyzes the hydrolysis of phospholipids to produce phosphate monoesters and diacylglycerol. it has many applications in the enzymatic degumming of plant oils. plc bc , a bacterial plc from bacillus cereus, is an optimal choice for this activity in terms of its wide substrate spectrum, high activity, and approved safety. unfortunately, its large-scale production and reliable high-throughput screening of plc bc remain challenging. herein, we summarize the research progress ...201626437973
antioedematogenic activity, acetylcholinesterase inhibition and antimicrobial properties of jacaranda oxyphylla.jacaranda oxyphylla cham. (bignoniaceae) is a shrub found in the brazilian cerrado and used in folk medicine to treat microbial infections. the aim of this study was to carry out a phytochemical screening and evaluate antioedematogenic, antimicrobial and antiacetylcholinesterase properties of j. oxyphylla crude extracts. all extracts analysed showed presence of terpenoids, which are potentially active chemical substances. a high ache inhibitory activity for hexane extract from leaves and for the ...201626469996
biomineralization of pb(ii) into pb-hydroxyapatite induced by bacillus cereus 12-2 isolated from lead-zinc mine tailings.the remediation of pb(ii) through biomineralization is rergarded as a promising technique as well as an interesting phenomenon for transforming heavy metals from mobile species into very stable minerals in the environment. studies are well needed for in-depth understanding the mechanism of pb(ii) immobilized by bacteria. in the present study, we investigated the uptake and biomineralization of pb(ii) using bacillus cereus 12-2 isolated from lead-zinc mine tailings. the maximum pb(ii) uptake capa ...201626468754
characterization of microfouling and corrosive bacterial community of a firewater distribution system.this investigation provides generic information on the culturable corrosive and the microfouling bacterial community in a firewater distribution system that uses freshwater. conventional microbiological methods were used for the selective isolation of the major microfouling bacteria. the isolates were characterized by 16s rrna gene sequencing and the biofilm as well as the corrosion characteristics of the isolates were evaluated. pseudomonas aeruginosa and bacillus cereus were predominantly obse ...201626467696
inactivation of bacillus cereus spores in a tsuyu sauce using continuous ohmic heating with five sequential elbow-type electrodes.the effect of ohmic heating (oh) in a pilot plant system which had a zig-zag shaped (elbow-type) ohmic heater with five sequential voltage electrodes was investigated on bacillus cereus spores in a commercial tsuyu sauce.201626497155
phytochemical, sub-acute toxicity, and antibacterial evaluation of cordia sebestena leaf extracts.in nigeria, cordia sebestena (boraginaceae), an understudied medicinal plant, is used in traditional medicine for the treatment of gastrointestinal disorders. in this study, we investigated the chemical composition, antibacterial potential, and sub-acute toxicity of c. sebestena leaves.201626501159
role of plant growth promoting rhizobacteria and ag-nano particle in the bioremediation of heavy metals and maize growth under municipal wastewater irrigation.the investigation evaluated the role of plant growth promoting rhizobacteria (pgpr) and ag-nano particle on the growth and metabolism of maize irrigated with municipal wastewater (mw). three pgpr isolated from mw were identified on the basis of 16s-rrna gene sequence analyses as pseudomonas sp., pseudomonas fluorescence, and bacillus cereus. the municipal waste water was used to irrigate the maize seeds inoculated with 3 isolated pgpr. the isolated pgpr had catalase and oxidase enzymes, solubili ...201626507686
production of co-polymers of polyhydroxyalkanoates by regulating the hydrolysis of biowastes.production of polyhydroxyalkanoate (pha) co-polymers by bacillus spp. was studied by feeding defined volatile fatty acids (vfas) obtained through controlled hydrolysis of various wastes. eleven mixed hydrolytic cultures (mhcs) each containing 6 strains could generate vfa from slurries of (2% total solids): pea-shells (ps), potato peels (pp), apple pomace (ap) and onion peels (op). ps hydrolysates (obtained with mhc2 and mhc5) inoculated with bacillus cereus egu43 and bacillus thuringiensis egu45 ...201626512866
detection of viable enterotoxin-producing bacillus cereus and analysis of toxigenicity from ready-to-eat foods and infant formula milk powder by multiplex pcr.bacillus cereus is responsible for several outbreaks of foodborne diseases due to its emetic toxin and enterotoxin. enterotoxins, cytotoxin k (cytk), nonhemolytic enterotoxin (nhe), and hemolysin bl (hbl), have been recorded in several diarrheal cases due to food poisoning from b. cereus. the objective of this study was to develop a rapid and accurate method that combines multiplex pcr with propidium monoazide to selectively detect viable cells of enterotoxin-producing b. cereus in milk powder, ...201626686715
microgravity-driven remodeling of the proteome reveals insights into molecular mechanisms and signal networks involved in response to the space flight environment.space is a hostile environment characterized by high vacuum, extreme temperatures, meteoroids, space debris, ionospheric plasma, microgravity and space radiation, which all represent risks for human health. a deep understanding of the biological consequences of exposure to the space environment is required to design efficient countermeasures to minimize their negative impact on human health. recently, proteomic approaches have received a significant amount of attention in the effort to further s ...201626571091
bacillus cereus infection: 57 case patients and a literature review.we aimed to study the characteristics of patients presenting with a bacillus cereus infection in a university hospital.201626525185
dual-recognition detection of staphylococcus aureus using vancomycin-functionalized magnetic beads as concentration carriers.vancomycin, which has a strong antibacterial effect to gram-positive bacteria, was adopted as one molecular recognition agent for bacterial detection. magnetic beads (mbs) were functionalized with this antibiotic to effectively concentrate staphylococcus aureus (s. aureus). in addition, alkaline phosphatase (alp)-tagged rabbit immunoglobulin g (alp-igg) was used as the second recognition agent to improve the specificity based on the binding between the fc region of rabbit igg and protein a in th ...201626606309
production of rhamnolipids and diesel oil degradation by bacteria isolated from soil contaminated by petroleum.biosurfactants are microbial secondary metabolites. the most studied are rhamnolipids, which decrease the surface tension and have emulsifying capacity. in this study, the production of biosurfactants, with emphasis on rhamnolipids, and diesel oil degradation by 18 strains of bacteria isolated from waste landfill soil contaminated by petroleum was analyzed. among the studied bacteria, gram-positive endospore forming rods (39%), gram positive rods without endospores (17%), and gram-negative rods ...201626588432
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