Publications

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combined genomic and genetic data integration of major agronomical traits in bread wheat (triticum aestivum l.).the high resolution integration of bread wheat genetic and genomic resources accumulated during the last decades offers the opportunity to unveil candidate genes driving major agronomical traits to an unprecedented scale. we combined 27 public quantitative genetic studies and four genetic maps to deliver an exhaustive consensus map consisting of 140,315 molecular markers hosting 221, 73, and 82 quantitative trait loci (qtl) for respectively yield, baking quality, and grain protein content (gpc) ...201729184557
genotyping-by-sequencing derived high-density linkage map and its application to qtl mapping of flag leaf traits in bread wheat.winter wheat parents 'harry' (drought tolerant) and 'wesley' (drought susceptible) were used to develop a recombinant inbred population with future goals of identifying genomic regions associated with drought tolerance. to precisely map genomic regions, high-density linkage maps are a prerequisite. in this study genotyping-by- sequencing (gbs) was used to construct the high-density linkage map. the map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cm with an average dis ...201729180623
exploring the genetics of fertility restoration controlled by rf1 in common wheat (triticum aestivum l.) using high-density linkage maps.hybrid wheat breeding has the potential to significantly increase wheat productivity compared to line breeding. the induction of male sterility by the cytoplasm of triticum timopheevii zhuk. is a widely discussed approach to ensure cross-pollination between parental inbred lines in hybrid wheat seed production. as fertility restoration in hybrids with this cytoplasm is often incomplete, understanding the underlying genetics is a prerequisite to apply this technology. a promising component for fe ...201729177892
structural variations in wheat hkt1;5 underpin differences in na+ transport capacity.an important trait associated with the salt tolerance of wheat is the exclusion of sodium ions (na+) from the shoot. we have previously shown that the sodium transporters tmhkt1;5-a and tahkt1;5-d, from triticum monoccocum (tm) and triticum aestivum (ta), are encoded by genes underlying the major shoot na+-exclusion loci nax1 and kna1, respectively. here, using heterologous expression, we show that the affinity (k m) for the na+ transport of tmhkt1;5-a, at 2.66 mm, is higher than that of tahkt1; ...201729177534
genetic impact of rht dwarfing genes on grain micronutrients concentration in wheat.wheat is a major staple food crop providing about 20% of dietary energy and proteins, and food products made of whole grain wheat are a major source of micronutrients like zinc (zn), iron (fe), manganese (mn), magnesium (mg), vitamin b and e. wheat provides about 40% intake of essential micronutrients by humans in the developing countries relying on wheat based diets. varieties with genetically enhanced levels of grain micronutrient concentrations can provide a cost-effective and sustainable opt ...201729200604
advantages and limitations of multiple-trait genomic prediction for fusarium head blight severity in hybrid wheat (triticum aestivum l.).predictabilities for wheat hybrids less related to the estimation set were improved by shifting from single- to multiple-trait genomic prediction of fusarium head blight severity. breeding for improved fusarium head blight resistance (fhbr) of wheat is a very laborious and expensive task. fhbr complexity is mainly due to its highly polygenic nature and because fhb severity (fhbs) is greatly influenced by the environment. associated traits plant height and heading date may provide additional info ...201729198016
site-specific transfer of chromosomal segments and genes in wheat engineered chromosomes.recently, engineered minichromosomes have been produced using a telomere-mediated truncation technique in some plants. however, the study on transferring genes to minichromosomes is very limited. here, telomere-mediated truncation was successfully performed in common wheat (triticum aestivum) to generate stable truncated chromosomes accompanied by a relatively high frequency of chromosomal rearrangements. after the cross between transgenic parents, a promoter-less dsred gene in a chromosome from ...201729169922
genome-wide association mapping of black point reaction in common wheat (triticum aestivum l.).black point is a serious threat to wheat production and can be managed by host resistance. marker-assisted selection (mas) has the potential to accelerate genetic improvement of black point resistance in wheat breeding. we performed a genome-wide association study (gwas) using the high-density wheat 90 k and 660 k single nucleotide polymorphism (snp) assays to better understand the genetic basis of black point resistance and identify associated molecular markers.201729169344
the role of reproductive isolation in allopolyploid speciation patterns: empirical insights from the progenitors of common wheat.the ability to cause reproductive isolation often varies among individuals within a plant species. we addressed whether such polymorphism influenced speciation of the allopolyploid common wheat (triticum aestivum l., aabbdd genome) by evaluating the expression of pre-pollination (outcrossing potential) and post-pollination (crossability) barriers in aegilops tauschii coss. (the d genome progenitor). in total, 201 ae. tauschii accessions representing the entire natural habitat range of the specie ...201729167543
biochar effects on uptake of cadmium and lead by wheat in relation to annual precipitation: a 3-year field study.biochar has been widely studied for its ability to reduce plant uptake of heavy metals by lowering metal bioavailabilities through adsorption and ph-driven fixation reactions. however, the long-term effect of biochar on heavy metal bioavailabilities in alkaline soils under natural redox condition is rarely studied. here, we report a study examining the effects of biochar on bioavailability and partitioning of cadmium (cd) and lead (pb) among different soil fractions over 3 years in a field study ...201729151186
a novel allele of tagw2-a1 is located in a finely mapped qtl that increases grain weight but decreases grain number in wheat (triticum aestivum l.).a novel tagw2-a1 allele was identified from a stable, robust qtl region, which is pleiotropic for thousand grain weight, grain number per spike, and grain morphometric parameters in wheat. thousand grain weight (tgw) and grain number per spike (gns) are two crucial determinants of wheat spike yield, and genetic dissection of their relationships can help to fine-tune these two components and maximize grain yield. by evaluating 191 recombinant inbred lines in 11 field trials, we identified five ge ...201729150697
identification and expression profiling of pht1 phosphate transporters in wheat in controlled environments and in the field.phosphorus (p) is an important macronutrient with critical functions in plants. phosphate (pi) transporters, which mediate pi acquisition and pi translocation within the plant, are key factors in pi deficiency responses. however, their relevance for adaptation to long-term pi limitation under agronomic conditions, particularly in wheat, remains unknown. here, we describe the identification of the complete pi transporter gene family (pht1) in wheat (triticum aestivum). gene expression profiles we ...201729148171
arbuscular mycorrhizal strategy for zinc mycoremediation and diminished translocation to shoots and grains in wheat.mycoremediation is an on-site remediation strategy, which employs fungi to degrade or sequester contaminants from the environment. the present work focused on the bioremediation of soils contaminated with zinc by the use of a native mycorrhizal fungi (am) called funneliformis geosporum (nicol. & gerd.) walker & schüßler. experiments were performed using triticum aestivum l. cv. gemmeza-10 at different concentrations of zn (50, 100, 200 mg kg-1) and inoculated with or without f. geosporum. the re ...201729145471
genome sequence of the progenitor of the wheat d genome aegilops tauschii.aegilops tauschii is the diploid progenitor of the d genome of hexaploid wheat (triticum aestivum, genomes aabbdd) and an important genetic resource for wheat. the large size and highly repetitive nature of the ae. tauschii genome has until now precluded the development of a reference-quality genome sequence. here we use an array of advanced technologies, including ordered-clone genome sequencing, whole-genome shotgun sequencing, and bionano optical genome mapping, to generate a reference-qualit ...201729143815
streptomyces tritici sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (triticum aestivum l.).two novel actinomycete isolates, designated strains neau-a4t and neau-a3, were isolated from rhizosphere soil of wheat (triticumaestivum l.) and characterized using a polyphasic approach. morphological and chemotaxonomic characteristics of the two strains coincided with those of the genus streptomyces. the 16s rrna gene sequence analysis showed that the two isolates exhibited 99.6 % 16s rrna gene sequence similarity with each other and that they were most closely related to streptomyces violaceo ...201729143728
features of ppd-b1 expression regulation and their impact on the flowering time of wheat near-isogenic lines.photoperiod insensitive ppd-1a alleles determine early flowering of wheat. increased expression of homoeologous ppd-d1a and ppd-a1a result from deletions in the promoter region, and elevated expression of ppd-b1a is determined by an increased copy number.201729143607
fine organization of genomic regions tagged to the 5s rdna locus of the bread wheat 5b chromosome.the multigene family encoding the 5s rrna, one of the most important structurally-functional part of the large ribosomal subunit, is an obligate component of all eukaryotic genomes. 5s rdna has long been a favored target for cytological and phylogenetic studies due to the inherent peculiarities of its structural organization, such as the tandem arrays of repetitive units and their high interspecific divergence. the complex polyploid nature of the genome of bread wheat, triticum aestivum, and the ...201729143604
molecular and agro-morphological characterization of ancient wheat landraces of turkey.turkey is one of the important gene centers for many crop species. in this research, some ancient wheats such as tetraploid and diploid hulled wheats together with hexaploid tir wheats (triticum aestivum ssp. leucospermum korn.) landraces mainly adapted to harsh winter conditions of eastern anatolian region of turkey were characterized at agro-morphological and molecular level. totally 50 hulled wheat population from kastamonu, konya and kayseri provinces and 15 tir wheats from kars provinces of ...201729143602
gwas for plant growth stages and yield components in spring wheat (triticum aestivum l.) harvested in three regions of kazakhstan.spring wheat is the largest agricultural crop grown in kazakhstan with an annual sowing area of 12 million hectares in 2016. annually, the country harvests around 15 million tons of high quality grain. despite environmental stress factors it is predicted that the use of new technologies may lead to increases in productivity from current levels of 1.5 to up to 3 tons per hectare. one way of improving wheat productivity is by the application of new genomic oriented approaches in plant breeding pro ...201729143598
genome-wide association study reveals novel genes associated with culm cellulose content in bread wheat (triticum aestivum, l.).plant cell wall formation is a complex, coordinated and developmentally regulated process. cellulose is the most dominant constituent of plant cell walls. because of its paracrystalline structure, cellulose is the main determinant of mechanical strength of plant tissues. as the most abundant polysaccharide on earth, it is also the focus of cellulosic biofuel industry. to reduce culm lodging in wheat and for improved ethanol production, delineation of the variation for stem cellulose content coul ...201729163625
genome-wide association study of calcium accumulation in grains of european wheat cultivars.mineral concentrations in cereals are important for human health, especially for people who depend mainly on consuming cereal diet. in this study, we carried out a genome-wide association study (gwas) of calcium concentrations in wheat (triticum aestivum l.) grains using a european wheat diversity panel of 353 varieties [339 winter wheat (ww) plus 14 of spring wheat (sw)] and phenotypic data based on two field seasons. high genotyping densities of single-nucleotide polymorphism (snp) markers wer ...201729163559
development of an efficient and reproducible regeneration system in wheat (triticum aestivum l.).the availability of reproducible regeneration system through tissue culture is a major bottleneck in wheat improvement program. the present study has considered to develop an efficient callus induction and regeneration system using mature and immature embryos as explants in recently released agronomically superior spring wheat varieties. an efficient sterilization process was standardized using 0.1% hgcl2 and 70% ethanol for both seeds and embryos. the maximum possible combinations of plant grow ...201729158641
performance indices in wheat chlorophyll a fluorescence and protein quality influenced by fhb.very little is known about the physiological interactions between wheat quality and fusarium head blight (fhb), which substantially reduces wheat grain yield and quality worldwide. in order to investigate stress-induced changes in flag leaves from plants artificially inoculated with fusarium, we screened for chlorophyll a fluorescence transient at 1, 2, 4, 7 and 14 days after fusarium inoculation. our results indicate that the maximum quantum yield of photochemistry (fv/fm) and the performance i ...201729156605
cytogenetic analysis and mapping of leaf rust resistance in aegilops speltoides tausch derived bread wheat line selection2427 carrying putative gametocidal gene(s).leaf rust (puccinia triticina) is a major biotic stress affecting wheat yields worldwide. host-plant resistance is the best method for controlling leaf rust. aegilops speltoides is a good source of resistance against wheat rusts. to date, five lr genes, lr28, lr35, lr36, lr47, and lr51, have been transferred from ae. speltoides to bread wheat. in selection2427, a bread wheat introgresed line with ae. speltoides as the donor parent, a dominant gene for leaf rust resistance was mapped to the long ...201729125785
isolation and molecular cytogenetic characterization of a wheat - leymus mollis double monosomic addition line and its progenies with resistance to stripe rust.a common wheat - leymus mollis (2n = 4x = 28, nsnsxmxm) double monosomic addition line, m11003-4-3-8/13/15 (2n = 44 = 42t.a + l.m2 + l.m3), with stripe rust resistance was developed (where t.a represents triticum aestivum chromosome, l.m represents l. mollis chromosome, and l.m2/3 represents l. mollis chromosome of homoeologous groups 2 and 3). the progenies of line m11003-4-3-8/13/15 were characterized by cytological observation, specific molecular markers, fluorescence in situ hybridization (f ...201729125778
uptake, translocation, and biotransformation of organophosphorus esters in wheat (triticum aestivum l.).the uptake, translocation and biotransformation of organophosphate esters (opes) by wheat (triticum aestivum l.) were investigated by a hydroponic experiment. the results demonstrated that opes with higher hydrophobicity were more easily taken up by roots, and opes with lower hydrophobicity were more liable to be translocated acropetally. a total of 43 metabolites including dealkylated, oxidatively dechlorinated, hydroxylated, methoxylated, and glutathione-, and glucuronide- conjugated products ...201729125288
effect of nitrogen levels and nitrogen ratios on lodging resistance and yield potential of winter wheat (triticum aestivum l.).lodging is one of the constraints that limit wheat yields and quality due to the unexpected bending or breaking stems on wheat (triticum aestivum l.) production worldwide. in addition to choosing lodging resistance varieties, husbandry practices also have a significant effect on lodging. nitrogen management is one of the most common and efficient methods. a field experiment with yangmai 20 as research material (a widely-used variety) was conducted to study the effects of different nitrogen level ...201729117250
gene encoding vesicle-associated membrane protein-associated protein from triticum aestivum (tavap) confers tolerance to drought stress.abiotic stresses like drought, salinity, high and low temperature, and submergence are major factors that limit the crop productivity. hence, identification of genes associated with stress response in crops is a prerequisite for improving their tolerance to adverse environmental conditions. in an earlier study, we had identified a drought-inducible gene, vesicle-associated membrane protein-associated protein (tavap), in developing grains of wheat. in this study, we demonstrate that tavap is able ...201729116579
[myc gene family in cereals: transformations during evolution of hexaploid bread wheat and its relatives].the transcription factors of the myc gene family are an integral part of the myb + myc + wd40 regulatory complex required to activate the genes of plant flavonoid biosynthesis. the tamyc1 gene, which controls the synthesis of flavonoid pigments in the grain pericarp, is known in bread wheat (triticum aestivum l., bbaadd genome, 2n = 6x = 42). in the present work, we identified 10 copies of this gene in the t. aestivum genome, 22 copies in the nearest bread wheat relatives (t. durum, t. urartu, t ...201729116063
early plant growth and biochemical responses induced by azospirillum brasilense sp245 lipopolysaccharides in wheat (triticum aestivum l.) seedlings are attenuated by procyanidin b2.this study analyzes the effects of procyanidin b2 on early wheat plant growth and plant biochemical responses promoted by lipopolysaccharides (lps) derived from the rhizobacteria azospirillum brasilense sp245. measurements of leaf, root length, fresh weight, and dry weight showed in vitro plant growth stimulation 4 days after treatment with a. brasilense as well as lps. superoxide anion (o2·-) and hydrogen peroxide (h2o2) levels increased in seedling roots treated with lps (100 μg ml-1). the chl ...201729110138
poaceae-specific ms1 encodes a phospholipid-binding protein for male fertility in bread wheat.male sterility is an essential trait in hybrid seed production for monoclinous crops, including rice and wheat. however, compared with the high percentage of hybrid rice planted in the world, little commercial hybrid wheat is planted globally as a result of the lack of a suitable system for male sterility. therefore, understanding the molecular nature of male fertility in wheat is critical for commercially viable hybrid wheat. here, we report the cloning and characterization of male sterility 1 ...201729109252
fusarium crown rot caused by fusarium pseudograminearum in cereal crops: recent progress and future prospects.diseases caused by fusarium pathogens inflict major yield and quality losses on many economically important plant species worldwide, including cereals. fusarium crown rot (fcr) caused by fusarium pseudograminearum is a cereal disease that occurs in many arid and semi-arid cropping regions of the world. in recent years, this disease has become more prevalent, in part due to the adoption of moisture-preserving cultural practices such as minimum tillage and stubble retention. in this pathogen profi ...201729105256
physical localization of a locus from agropyron cristatum conferring resistance to stripe rust in common wheat.stripe rust, caused by puccinia striiformis f. sp. tritici (pst), is one of the most destructive diseases of wheat (triticum aestivum l.) worldwide. agropyron cristatum (l.) gaertn. (2n = 28, pppp), one of the wild relatives of wheat, exhibits resistance to stripe rust. in this study, wheat-a. cristatum 6p disomic addition line 4844-12 also exhibited resistance to stripe rust. to identify the stripe rust resistance locus from a. cristatum 6p, ten translocation lines, five deletion lines and the ...201729137188
glycomyces rhizosphaerae sp. nov., isolated from the root and rhizosphere soil of wheat (triticum aestivum l.).two actinomycete strains, neau-c11t and neau-c8, isolated from rhizosphere soil and wheat root, respectively, collected from langfang, hebei province, china. a polyphasic study was carried out to establish the taxonomic position of the two strains. morphological and chemotaxonomic characteristics of the isolates coincided with the genus glycomyces. sequences analysis of the 16s rrna gene also showed that the organisms belong to the genus glycomyces and glycomyces algeriensis is the highest seque ...201729134931
transcriptome dynamics provide insights into long-term salinity stress tolerance in triticum aestivum cv. kharchia local.kharchia local, a wheat (triticum aestivum) cultivar, is native to the saline-sodic soils of pali district, rajasthan, india and well known for its salinity stress tolerance. in the present study, we performed transcriptome sequencing to compare genome wide differential expression pattern between flag leaves of salinity stressed (15 ec) and control plants at anthesis stage. the 63.9 million paired end raw reads were assembled into 74,106 unigenes, of which, 3197 unigenes were found to be differe ...201729102901
from genetic stock to genome editing: gene exploitation in wheat.bread wheat (triticum aestivum) ranks as one of our most important staple crops. however, its hexaploid nature has complicated our understanding of the genetic bases underlying many of its traits. historically, functional genetic studies in wheat have focused on identifying natural variations and have contributed to assembling and enriching its genetic stock. recently, mold-breaking advances in whole genome sequencing, exome-capture based mutant libraries, and genome editing have revolutionized ...201729102241
genome-wide analysis of the cellulose synthase-like (csl) gene family in bread wheat (triticum aestivum l.).hemicelluloses are a diverse group of complex, non-cellulosic polysaccharides, which constitute approximately one-third of the plant cell wall and find use as dietary fibres, food additives and raw materials for biofuels. genes involved in hemicellulose synthesis have not been extensively studied in small grain cereals.201729100539
transfer of a starch phenotype from wild wheat to bread wheat by deletion of a locus controlling b-type starch granule content.our previous genetic analysis of a tetraploid wild wheat species, aegilops peregrina, predicted that a single gene per haploid genome, bgc-1, controls b-type starch granule content in the grain. to test whether bread wheat (triticum aestivum l.) has orthologous bgc-1 loci, we screened a population of γ-irradiated bread wheat cv. paragon for deletions of the group 4 chromosomes spanning bgc-1. suitable deletions, each encompassing ~600-700 genes, were discovered for chromosomes 4a and 4d. these t ...201729099990
impact of grazing dairy steers on winter rye (secale cereale) versus winter wheat (triticum aestivum) and effects on meat quality, fatty acid and amino acid profiles, and consumer acceptability of organic beef.meat from holstein and crossbred organic dairy steers finished on winter rye and winter wheat pastures was evaluated and compared for meat quality, fatty acid and amino acid profiles, and consumer acceptability. two adjacent 4-ha plots were established with winter rye or winter wheat cover crops in september 2015 at the university of minnesota west central research and outreach center (morris, mn). during spring of 2015, 30 steers were assigned to one of three replicate breed groups at birth. br ...201729099863
the content of tocols in south african wheat; impact on nutritional benefits.wheat is a major component within human consumption, and due to the large intake of wheat, it has an impact on human nutritional health. this study aimed at an increased understanding of how the content and composition of tocols may be governed for increased nutritional benefit of wheat consumption. therefore, ten south african wheat cultivars from three locations were fractionated into white and whole flour, the content and concentration of tocols were evaluated by high performance liquid chrom ...201729099067
wheat chronic exposure to tio2-nanoparticles: cyto- and genotoxic approach.this study investigates the phytotoxicity of chronic exposure (up to 20 d) of different tio2 nanoparticles (tio2-np) concentrations (5, 50, 150 mg l-1) in triticum aestivum. germination was not affected by tio2-np exposure and seedling shoot length (3 d) was enhanced. contrarily, plants' shoot growth (20 d) was impaired. effects on membrane permeability and total antioxidant capacity in tio2-np chronic exposure were organ dependent: increased in leaves and decreased in roots. roots also showed l ...201729096177
effects of bacillus subtilis on some physiological and biochemical parameters of triticum aestivum l. (wheat) under salinity.endophytic strain bacillus subtilis (b. subtilis) 10-4, producing indole-3-acetic acid (iaa) and siderofores but not active in phosphate solubilization, exerted a protective effect on triticum aestivum l. (wheat) plant grown under salinity (2% nacl) stress. exposure to salt stress resulted in an essential increase of proline (pro) and malondialdehyde (mda) level in the seedlings. at the same time the seedlings inoculated with b. subtilis 10-4 were characterized by decreased level of stress-induc ...201729096176
chromosomal constitutions of five wheat - elytrigia elongata partial amphiploids as revealed by gish, multicolor gish and fish.a combination of meiotic pairing analysis and in situ hybridization (genomic in situ hybridization [gish], multicolor gish [mcgish] and fluorescence in situ hybridization [fish]) of five triticum aestivum (linnaeus, 1753) - elytrigia elongata (podpěra, 1902) (2n = 10x = 70) amphiploids was employed to investigate the genomic constitution and relationships between wheat and alien chromosomes. gish, multicolor gish and fish patterns of mitotic chromosomes indicate that the genomic constitution of ...201729093801
multiple mechanisms are involved in new imazamox-resistant varieties of durum and soft wheat.weed control in wheat is one of the major goals of farmers in their efforts toward obtaining the highest crop yields for human foods. several studies (dose-response, enzyme activity, absorption-translocation and metabolism) were conducted to characterize the resistance level of two new wheat cultivars called rafalín (triticum aestivum) and antoñín (t. durum) that were obtained by conventional breeding based on clearfield® technology; they are resistant (r) to imazamox compared to their sensitive ...201729093532
using synchrotron-based approaches to examine the foliar application of znso4 and zno nanoparticles for field-grown winter wheat.the effects of foliar-applied zno nanoparticles (zno nps) and znso4 on the winter wheat (triticum aestivum l.) grain yield and grain quality were studied under field conditions, with the distribution and speciation of zn within the grain examined using synchrotron-based x-ray fluorescence microscopy and x-ray absorption spectroscopy. although neither of the two zn compounds improved the grain yield or quality, both increased the grain zn concentration (average increments were 5 and 10 mg/kg for ...201729091444
the e3 ligase tasap5 alters drought stress responses by promoting the degradation of drip proteins.in arabidopsis (arabidopsis thaliana) plants growing under normal conditions, dehydration-responsive element binding protein2a (dreb2a) is present at low levels because it is ubiquitinated and destabilized by dreb2a interacting protein1 (drip1) and drip2 through 26s proteasome-mediated proteolysis. drought stress counteracts the ubiquitination and proteolysis of dreb2a, thus allowing the accumulation of sufficient amounts of dreb2a protein to activate downstream gene expression. the mechanisms l ...201729089392
evolution of the aux/iaa gene family in hexaploid wheat.the aux/iaa (iaa) gene family, involved in the auxin signalling pathway, acts as an important regulator in plant growth and development. in this study, we explored the evolutionary trajectory of the iaa family in common wheat. the results showed ten pairs of paralogs among 34 taiaa family members. seven of the pairs might have undergone segmental duplication, and the other three pairs appear to have experienced tandem duplication. except for taiaa15-16, these duplication events occurred in the a ...201729085968
allelic analysis of low molecular weight glutenin subunits using 2-dge in korean wheat cultivars.two-dimensional gel electrophoresis (2-dge) was used as a complement to sds-page to determine the allelic compositions of lmw-gs in 32 korean wheat cultivars. protein patterns generated by 2-dge from each cultivar were compared to patterns from standard wheat cultivars for each allele. at the glu-a3 locus, thirteen c, twelve d, three e (null), two g and two new alleles were identified. at the glu-b3 locus, one b, nineteen d, four h, one i and five ad alleles were identified. at the glu-d3 locus, ...201729085250
potage: a visualisation tool for speeding up gene discovery in wheat.popseq ordered triticum aestivum gene expression (potage) is a web application which accelerates the process of identifying candidate genes for quantitative trait loci (qtl) in hexaploid wheat. this is achieved by leveraging several of the most commonly used data sets in wheat research. these include the chromosome survey sequences, their order along the chromosomes determined by the population sequencing (popseq) approach, the gene predictions and rna-seq expression data. potage aggregates thos ...201729085014
genome-wide analysis of wheat calcium atpases and potential role of selected acas and ecas in calcium stress.p2- type calcium atpases (acas-auto inhibited calcium atpases and ecas-endoplasmic reticulum calcium atpases) belong to the p- type atpase family of active membrane transporters and are significantly involved in maintaining accurate levels of ca2+, mn2+ and zn2+ in the cytosol as well as playing a very important role in stress signaling, stomatal opening and closing and pollen tube growth. here we report the identification and possible role of some of these atpases from wheat.201729078753
identification and characterization of sr13, a tetraploid wheat gene that confers resistance to the ug99 stem rust race group.the puccinia graminis f. sp. tritici (pgt) ug99 race group is virulent to most stem rust resistance genes currently deployed in wheat and poses a threat to global wheat production. the durum wheat (triticum turgidum ssp. durum) gene sr13 confers resistance to ug99 and other virulent races, and is more effective at high temperatures. using map-based cloning, we delimited a candidate region including two linked genes encoding coiled-coil nucleotide-binding leucine-rich repeat proteins designated c ...201729078294
agronomic traits and molecular marker identification of wheat-aegilops caudata addition lines.aegilops caudata is an important gene source for wheat breeding. intensive evaluation of its utilization value is an essential first step prior to its application in breeding. in this research, the agronomical and quality traits of triticum aestivum-ae. caudata additions b-g (homoeologous groups not identified) were analyzed and evaluated. disease resistance tests showed that chromosome d of ae. caudata might possess leaf rust resistance, and chromosome e might carry stem rust and powdery mildew ...201729075275
host-induced gene silencing of the mapkk gene psfuz7 confers stable resistance to wheat stripe rust.rna interference (rnai) is a powerful genetic tool to accelerate research in plant biotechnology and control biotic stresses by manipulating target gene expression. however, the potential of rnai in wheat to efficiently and durably control the devastating stripe rust fungus puccinia striiformis f. sp. tritici (pst) remained largely under explored so far. to address this issue, we generated transgenic wheat (triticum aestivum) lines expressing dsrna targeting psfuz7 transcripts of pst we analyzed ...201729070517
the first near-complete assembly of the hexaploid bread wheat genome, triticum aestivum.common bread wheat, triticum aestivum, has one of the most complex genomes known to science, with 6 copies of each chromosome, enormous numbers of near-identical sequences scattered throughout, and an overall haploid size of more than 15 billion bases. multiple past attempts to assemble the genome have produced assemblies that were well short of the estimated genome size. here we report the first near-complete assembly of t. aestivum, using deep sequencing coverage from a combination of short il ...201729069494
the potential role of selected bioactive compounds from spelt and common wheat in glycemic control.diet is an important lifestyle factor which influences people's health and the prevention of chronic diseases such as type 2 diabetes. cereal-based foods constitute the main component of the everyday diet worldwide. old cereal species like spelt (triticum spelta l.) are becoming more and more popular, especially in europe. this review focuses on the role of bioactive compounds from spelt and their possible biological mechanisms of action in glycemic control. spelt grain contains a high amount of ...201729068605
physical location of new pcr-based markers and powdery mildew resistance gene(s) on rye (secale cereale l.) chromosome 4 using 4r dissection lines.rye (secale cereale l.) 4r chromosome contains elite genes that are applicable for wheat (triticum aestivum l.) cultivar improvement. pcr-based 4r-specific markers can benefit the detection of elite genes on 4r in wheat backgrounds. in this study, a new fluorescence in situ hybridization (fish) map of the 4rku chromosome of rye kustro has been constructed. a set of 4rku dissection lines was obtained and 301 new 4rku-specific markers were developed using specific length amplified fragment sequenc ...201729067030
in vivo phosphoproteome characterization reveals key starch granule-binding phosphoproteins involved in wheat water-deficit response.drought stress during grain development causes significant yield loss in cereal production. the phosphorylated modification of starch granule-binding proteins (sgbps) is an important mechanism regulating wheat starch biosynthesis. in this study, we performed the first proteomics and phosphoproteomics analyses of sgbps in elite chinese bread wheat (triticum aestivum l.) cultivar jingdong 17 under well-watered and water-stress conditions.201729058608
melatonin improves the photosynthetic carbon assimilation and antioxidant capacity in wheat exposed to nano-zno stress.the release of nanoparticles into the environment is inevitable, which has raised global environmental concern. melatonin is involved in various stress responses in plants. the present study investigated the effects of melatonin on photosynthetic carbon (c) assimilation and plant growth in nano-zno stressed plants. it was found that melatonin improved the photosynthetic c assimilation in nano-zno stressed wheat plants, mainly due to the enhanced photosynthetic energy transport efficiency, higher ...201729057793
heavy metal accumulation and health risk assessment in soil-wheat system under different nitrogen levels.heavy metal(loid)s (hms) in organic fertilizer have become a primary source of hms pollution of farmlands, which could cause deleterious health effects in people exposed through soil-plant systems via multi-pathways. this study investigated china's main grain production area (henan province) to evaluate the accumulation and transport characteristics of hms (cr, mn, ni, cu, zn, as, cd and pb) in a soil-wheat system and conduct a health risk assessment for wheat (triticum aestivum) grain under dif ...201729054646
genome-wide identification and analysis of mapk and mapkk gene families in bread wheat (triticum aestivum l.).the mitogen-activated protein kinase (mapk) cascade is a universal signal transduction module that plays a vital role in regulating growth and development, as well as environmental stress responses in plants. wheat is one of the most important crops worldwide. although the mapk kinase kinase (map3k) family in wheat has been investigated, the mapk and mapk kinase (map2k) gene families remain unknown at present. here, 54 mapk and 18 mapkk genes were identified in wheat using recent genomic informa ...201729053643
linear discriminant analysis reveals differences in root architecture in wheat seedlings related to nitrogen uptake efficiency.root architecture impacts water and nutrient uptake efficiency. identifying exactly which root architectural properties influence these agronomic traits can prove challenging. in this paper, approximately 300 wheat (triticum aestivum) plants were divided into four groups using two binary classifications, high versus low nitrogen uptake efficiency (nupe), and high versus low nitrate in the growth medium. the root system architecture for each wheat plant was captured using 16 quantitative variable ...201729048563
an updated protocol for high throughput plant tissue sectioning.quantification of the tissue and cellular structure of plant material is essential for the study of a variety of plant sciences applications. currently, many methods for sectioning plant material are either low throughput or involve free-hand sectioning which requires a significant amount of practice. here, we present an updated method to provide rapid and high-quality cross sections, primarily of root tissue but which can also be readily applied to other tissues such as leaves or stems. to incr ...201729046689
a quantitative genetic framework highlights the role of epistatic effects for grain-yield heterosis in bread wheat.increasing wheat yield is a key global challenge to producing sufficient food for a growing human population. wheat grain yield can be boosted by exploiting heterosis, the superior performance of hybrids compared with midparents. here we present a tailored quantitative genetic framework to study the genetic basis of midparent heterosis in hybrid populations derived from crosses among diverse parents. we applied this framework to an extensive data set assembled for winter wheat. grain yield was a ...201729038596
taflo2-a1, an ortholog of rice flo2, is associated with thousand grain weight in bread wheat (triticum aestivum l.).the flo2 gene is a member of a conserved gene family in plants. this gene has been found to be related to thousand grain weight (tgw) in rice. its orthologs in hexaploid wheat were cloned, and the haplotype variation in taflo2-a1 was tested for association with tgw.201729037166
commercial phenoxyacetic herbicides control heavy metal uptake by wheat in a divergent way than pure active substances alone.impact of two widely used commercial herbicides, i.e. aminopielik d 450 sl and chwastox 300 sl, on the uptake and translocation of selected heavy metals in wheat plants triticum aestivum l. cultivated in the laboratory pot experiments was investigated. mineral-humus, loamy sand soil representative for the central part of poland was applied. bioavailable, exchangeable and total forms of cd, co, cu, zn, pb, and mn were determined. transfer coefficients, translocation, and bioaccumulation factors i ...201729034141
high-resolution mapping of the pericentromeric region on wheat chromosome arm 5as harbouring the fusarium head blight resistance qtl qfhs.ifa-5a.the qfhs.ifa-5a allele, contributing to enhanced fusarium head blight resistance in wheat, resides in a low-recombinogenic region of chromosome 5a close to the centromere. a near-isogenic ril population segregating for the qfhs.ifa-5a resistance allele was developed and among 3650 lines as few as four recombined within the pericentromeric c-5as1-0.40 bin, yielding only a single recombination point. genetic mapping of the pericentromeric region using a recombination-dependent approach was thus no ...201729024288
qtls and potential candidate genes for heat stress tolerance identified from the mapping populations specifically segregating for fv/fm in wheat.despite the fact that fv/fm (maximum quantum efficiency of photosystem ii) is the most widely used parameter for a rapid non-destructive measure of stress detection in plants, there are barely any studies on the genetic understanding of this trait under heat stress. our aim was to identify quantitative trait locus (qtl) and the potential candidate genes linked to fv/fm for improved photosynthesis under heat stress in wheat (triticum aestivum l.). three bi-parental f2 mapping populations were gen ...201729021798
molecular identification of the wheat male fertility gene ms1 and its prospects for hybrid breeding.the current rate of yield gain in crops is insufficient to meet the predicted demands. capturing the yield boost from heterosis is one of the few technologies that offers rapid gain. hybrids are widely used for cereals, maize and rice, but it has been a challenge to develop a viable hybrid system for bread wheat due to the wheat genome complexity, which is both large and hexaploid. wheat is our most widely grown crop providing 20% of the calories for humans. here, we describe the identification ...201729021581
a conserved fungal glycosyltransferase facilitates pathogenesis of plants by enabling hyphal growth on solid surfaces.pathogenic fungi must extend filamentous hyphae across solid surfaces to cause diseases of plants. however, the full inventory of genes which support this is incomplete and many may be currently concealed due to their essentiality for the hyphal growth form. during a random t-dna mutagenesis screen performed on the pleomorphic wheat (triticum aestivum) pathogen zymoseptoria tritici, we acquired a mutant unable to extend hyphae specifically when on solid surfaces. in contrast "yeast-like" growth, ...201729020037
effect of silicate slag application on wheat grown under two nitrogen rates.field studies were established on the alluvial floodplain soils in louisiana, from 2013 to 2015, to evaluate the effect of silicate slag applications on productivity of wheat (triticum aestivum), under sufficient and high nitrogen (n) application rates. treatments were arranged in a randomized complete block design, with four replications consisting of twelve treatments: a factorial combination of two n (101 and 145 kg n ha-1) and five silicate slag rates (0, 1, 2, 4.5, and 9 mg ha-1), and two c ...201729019922
tapub1, a putative e3 ligase gene from wheat, enhances salt stress tolerance in transgenic nicotiana benthamiana.high salinity is one of the most severe environmental stresses and limits the growth and yield of diverse crop plants. we isolated a gene named tapub1 from wheat (triticum aestivum l. cv hf9703) that encodes a novel protein containing a u-box domain, the precursor rna processing 19p (prp19) superfamily and wd-40 repeats. real-time reverse transcription-pcr analysis showed that tapub1 transcript accumulation was up-regulated by high salinity, drought and phytohormones, suggesting that it plays a ...201729016965
aluminum induces distinct changes in the metabolism of reactive oxygen and nitrogen species in the roots of two wheat genotypes with different aluminum resistance.aluminum (al) toxicity in acid soils is a primary factor limiting plant growth and crop yield worldwide. considerable genotypic variation in resistance to al toxicity has been observed in many crop species. in wheat (triticum aestivum l.), al phytotoxicity is a complex phenomenon involving multiple physiological mechanisms which are yet to be fully characterized. to elucidate the physiological and molecular basis of al toxicity in wheat, we performed a detailed analysis of reactive oxygen specie ...201729016127
variations in yield and gluten proteins in durum wheat varieties under late-season foliar versus soil application of nitrogen fertilizer in a northern mediterranean environment.with the increasing demand for high-quality foodstuffs and concern for environmental sustainability, late-season nitrogen (n) foliar fertilization of common wheat is now an important and widespread practice. this study investigated the effects of late-season foliar versus soil n fertilization on yield and protein content of four varieties of durum wheat, aureo, ariosto, biensur and liberdur, in a three-year field trial in northern italy.201728992373
mesophyll cell ultrastructure of wheat leaves etiolated by lead and selenium.the ultrastructure of mesophyll cells was studied in leaves of the triticum aestivum l. cv. "trizo" seedlings after two weeks of growth on soil contaminated by pb and/or se. the soil treatments: control; (pb1) 50mgkg-1; (pb2) 100mgkg-1; (se1) 0.4mgkg-1; (se2) 0.8mgkg-1; (pb1+se1); (pb1+se2); (p2+se1); and (pb2+se2) were used. light and other conditions were optimal for plant growth. the (se1)-plants showed enhanced growth and biomass production; (pb1+se1)-plants did not lag behind the controls, ...201728988150
allelic variation and transcriptional isoforms of wheat tamyc1 gene regulating anthocyanin synthesis in pericarp.recently the tamyc1 gene encoding bhlh transcription factor has been isolated from the bread wheat (triticum aestivum l.) genome and shown to co-locate with the pp3 gene conferring purple pericarp color. as a functional evidence of tamyc1 and pp3 being the same, higher transcriptional activity of the tamyc1 gene in colored pericarp compared to uncolored one has been demonstrated. in the current study, we present additional strong evidences of tamyc1 to be a synonym of pp3. furthermore, we have f ...201728983311
rna-seq analysis provides the first insights into the phylogenetic relationship and interspecific variation between agropyron cristatum and wheat.agropyron cristatum, which is a wild grass of the tribe triticeae, grows widely in harsh environments and provides many desirable genetic resources for wheat improvement. however, unclear interspecific phylogeny and genome-wide variation has limited the utilization of a. cristatum in the production of superior wheat varieties. in this study, by sequencing the transcriptome of the representative tetraploid a. cristatum z559 and the common wheat variety fukuhokomugi (fukuho), which are often used ...201728983310
in vitro and in vivo anti-cancer activity of dichloromethane fraction of triticum aestivum sprouts.triticum aestivum sprouts (ta) contain significant amounts of chlorophyll, minerals, enzymes, and other functional entities. furthermore, ta extracts have been shown to possess anti-obesity, anti-diabetic and hepatoprotective effects and are believed to help blood flow, digestion, and general detoxification of the body. in this study, the mechanism underlying the anti-cancer effects of a dichloromethane fraction of ta (tdf) was investigated in vitro and in vivo. in vitro study was done by examin ...201728972884
 cinnamon rolls triggered anaphylactic reaction. the genesis could be wheat-dependent exercise-induced anaphylaxis two case reports from our department of allergology illustrate the wheat-dependent exercise-induced anaphylaxis (wdeia), which is a relatively rare, yet potentially fatal form of food allergy. wheat (triticum aestivum) is the most commonly grown crop world-wide, and some individuals cannot tolerate it due to wheat hypersensitivity. the most dangerous reaction is the anaphylaxis due t ...201728972639
health risk assessment and growth characteristics of wheat and maize crops irrigated with contaminated wastewater.the present study evaluated the effect of untreated wastewater irrigation and its health risks in triticum aestivum (wheat) and zea mays (maize) cultivated at south cairo, egypt. morphological measurements (stem and root lengths, number of leaves per plant, and dry weights of main organs) as well as soil, irrigation water, and plant analyses for nutrients and heavy metals were conducted in polluted and unpolluted sites. wastewater irrigations leads to reduction in the morphological traits of the ...201728971323
variation in foliar uptake of polycyclic aromatic hydrocarbons in six varieties of winter wheat.to investigate intraspecific variations of foliar uptake of polycyclic aromatic hydrocarbons (pahs) of winter wheat (triticum aestivum l.), leaves of six varieties including changwu 521 (cw), hedong tx-006 (hd), jiaomai 266 (jm), xiaoyan 22 (xy), yunong 949 (yn), and zhongmai 175 (zm) were exposed to three levels of (0, 0.25, and 1.5 mg l-1) mixture of six selected pahs (phenanthrene, anthracene, pyrene, benz[a]anthracene, benzo[a]pyrene, and benzo[g,h,i]perylene). after 10 consecutive days of a ...201728965195
low-molecular-weight glutenin subunits from the 1u genome of aegilops umbellulata confer superior dough rheological properties and improve breadmaking quality of bread wheat.wheat-related genomes may carry new glutenin genes with the potential for quality improvement of breadmaking. in this study, we estimated the gluten quality properties of the wheat line cnu609 derived from crossing between chinese spring (cs, triticum aestivum l., 2n = 6x = 42, aabbdd) and the wheat aegilops umbellulata (2n = 2x = 14, uu) 1u(1b) substitution line, and investigated the function of 1u-encoded low-molecular-weight glutenin subunits (lmw-gs).201728960410
rapid development and characterization of chromosome specific translocation line of thinopyrum elongatum with improved dough strength.the protein content and its type are principal factors affecting wheat (triticum aestivum) end product quality. among the wheat proteins, glutenin proteins, especially, high molecular weight glutenin subunits (hmw-gs) are major determinants of processing quality. wheat and its primary gene pool have limited variation in terms of hmw-gs alleles. wild relatives of wheat are an important source of genetic variation. for improvement of wheat processing quality its wild relative thinopyrum elongatum ...201728959271
genome-wide association study of septoria tritici blotch resistance in ethiopian durum wheat landraces.septoria tritici blotch (stb) is a devastating fungal disease affecting durum and bread wheat cultivation worldwide. the identification, development, and employment of resistant wheat genetic material is the key to overcoming costs and limitations of fungicide treatments. the search for resistance sources in untapped genetic material may speed up the deployment of stb genetic resistance in the field. ethiopian durum wheat landraces represent a valuable source of such diversity. in this study, 31 ...201728959268
ectopic expression of triticum aestivum serk genes (taserks) control plant growth and development in arabidopsis.somatic embryogenesis receptor kinases (serks) belong to a small gene family of receptor-like kinases involved in signal transduction. a total of 54 genes were shortlisted from the wheat genome survey sequence of which 5 were classified as serks and 49 were identified as serk-like (serls). tissue- specific expression of taserks at major developmental stages of wheat corroborates their indispensable role during somatic and zygotic embryogenesis. taserk transcripts show inherent differences in the ...201728959050
assessment of two carrier materials for phosphate solubilizing biofertilizers and their effect on growth of wheat (triticum aestivum l.).biofertilizers are usually carrier-based inoculants containing beneficial microorganisms. incorporation of microorganisms in carrier material enables easy-handling, long-term storage and high effectiveness of biofertilizers. objective of the present study was to assess enriched biogas sludge and soil as biofertilizer carriers on growth and yield of wheat. six phosphate solubilizing strains were used in this study. three phosphate solubilizing strains, 77-ns2 (bacillus endophyticus), 77-cs-s1 (ba ...201728942836
effects of co-composting of farm manure and biochar on plant growth and carbon mineralization in an alkaline soil.in the present study, the effects of co-composts of biochar (bc) and farm manure (fm) on the growth of wheat (triticum aestivum l.) and carbon mineralization in an alkaline soil were investigated. the co-composts of fm and bc were prepared at various ratios (fm100:bc0, fm75:bc25, fm50:bc50, fm25:bc75, fm0:bc100) using aboveground piles and were used in two separate experiments conducted simultaneously. in the plant growth trial, prepared co-composts were applied at a rate of 2% w/w and wheat was ...201728942511
silencing of copine genes confers common wheat enhanced resistance to powdery mildew.powdery mildew, caused by the biotrophic fungal pathogen blumeria graminis f. sp. tritici (bgt), is a major threat to the production of wheat (triticum aestivum). it is of great importance to identify new resistance genes for the generation of bgt-resistant or bgt-tolerant wheat varieties. here, we show that the wheat copine genes tabon1 and tabon3 negatively regulate wheat disease resistance to bgt. two copies of tabon1 and three copies of tabon3, located on chromosomes 6as, 6bl, 1al, 1bl and 1 ...201728941084
practical application of genomic selection in a doubled-haploid winter wheat breeding program.crop improvement is a long-term, expensive institutional endeavor. genomic selection (gs), which uses single nucleotide polymorphism (snp) information to estimate genomic breeding values, has proven efficient to increasing genetic gain by accelerating the breeding process in animal breeding programs. as for crop improvement, with few exceptions, gs applicability remains in the evaluation of algorithm performance. in this study, we examined factors related to gs applicability in line development ...201728936114
herbicidal activity of slow-release herbicide formulations in wheat stands infested by weeds.the present study reports the herbicidal activity of metribuzin and tribenuron-methyl embedded in the degradable matrix of natural poly-3-hydroxybutyrate [p(3hb)/met and p(3hb)/tbm]. the developed formulations were constructed as films and microgranules, which were tested against the weeds such as white sweet clover melilotus albus and lamb's quarters chenopodium album in the presence of soft spring wheat (triticum aestivum, cv. altaiskaya 70) as the subject crop for investigation. the activity ...201728934007
addition of aegilops u and m chromosomes affects protein and dietary fiber content of wholemeal wheat flour.cereal grain fiber is an important health-promoting component in the human diet. one option to improve dietary fiber content and composition in wheat is to introduce genes from its wild relatives aegilops biuncialis and aegilops geniculata. this study showed that the addition of chromosomes 2ug, 4ug, 5ug, 7ug, 2mg, 5mg, and 7mg of ae. geniculata and 3ub, 2mb, 3mb, and 7mb of ae. biuncialis into bread wheat increased the seed protein content. chromosomes 1ug and 1mg increased the proportion of po ...201728932231
diversity, distribution of puroindoline genes and their effect on kernel hardness in a diverse panel of chinese wheat germplasm.kernel hardness, which has great influence on the end-use properties of common wheat, is mainly controlled by puroindoline genes, pina and pinb. using ecotilling platform, we herein investigated the allelic variations of pina and pinb genes and their association with the single kernel characterization system (skcs) hardness index in a diverse panel of wheat germplasm.201728931378
probing behavior of dichelops furcatus (f.) (heteroptera: pentatomidae) on wheat plants characterized by electropenetrography (epg) and histological studies.the stink bug dichelops furcatus (f.) (heteroptera: pentatomidae) has increased in abundance in recent years on the wheat, triticum aestivum l., crop cultivated in the southern region of brazil. to investigate the probing (stylet penetration) behaviors and nonprobing behaviors of d. furcatus on wheat plants, the electrical penetration graph or electropenetrography (epg) technique was applied. nine epg waveforms (types/subtypes) were identified and described on stem and on ear head of wheat plant ...201728931161
quantitative trait loci responsible for sharp eyespot resistance in common wheat ci12633.sharp eyespot is a major fungal disease of wheat caused by rhizoctonia cerealis in cool and humid environments worldwide. in this study, 224 single seed descent derived f13, f14 and f15 recombinant inbred lines (rils) from the cross between ci12633 (a resistant cultivar) and yangmai 9 (a susceptible cultivar) were assessed for sharp eyespot resistance (r.cerealis isolate r0301) in field and greenhouse conditions in three growing seasons. different agronomic characteristics were also evaluated in ...201728924253
three myb genes co-regulate the phloem-based defence against english grain aphid in wheat.plant phloem-based defence (pbd) against phloem-feeding insects is characteristic of the sieve occlusion by phloem lectins and β-1,3-glucan callose, both of which are produced under regulation by ethylene and myb transcription factors. wheat pbd requires β-1,3-glucan synthase-like proteins gsl2, gsl10, and gsl12, and may also require insect-resistant mannose-binding lectins hfr-1 and wci-1, which can accumulate in the phloem upon aphid feeding. this study elucidates whether any of the 73 myb gen ...201728922762
the roles of pleiotropy and close linkage as revealed by association mapping of yield and correlated traits of wheat (triticum aestivum l.).grain yield (gy) of bread wheat (triticum aestivum l.) is quantitatively inherited. correlated gy-syndrome traits such as plant height (ph), heading date (hd), thousand grain weight (tgw), test weight (tw), grains per ear (gpe), and ear weight (ew) influence gy. most quantitative genetics studies assessed the multiple-trait (mt) complex of gy-syndrome using single-trait approaches, and little is known about its underlying pleiotropic architecture. we investigated the pleiotropic architecture of ...201728922760
genome-wide identification, classification, evolutionary analysis and gene expression patterns of the protein kinase gene family in wheat and aegilops tauschii.in this study we systematically identified and classified pks in triticum aestivum, triticum urartu and aegilops tauschii. domain distribution and exon-intron structure analyses of pks were performed, and we found conserved exon-intron structures within the exon phases in the kinase domain. collinearity events were determined, and we identified various t. aestivum pks from polyploidizations and tandem duplication events. global expression pattern analysis of t. aestivum pks revealed that some pk ...201728918554
drug-herb interactions in the elderly patient with ibd: a growing concern.inflammatory bowel disease (ibd), which includes conditions such as crohn's disease and ulcerative colitis, is becoming more prevalent with the elderly being the fastest growing group. parallel to this, there is an increasing interest in the use of complementary and alternative medicine (cam). nearly half of patients with ibd have used cam at one time. the elderly patients, however, are burdened by comorbid conditions, polypharmacy, and altered functional status. with increasing use of complemen ...201728918484
seed maturation associated transcriptional programs and regulatory networks underlying genotypic difference in seed dormancy and size/weight in wheat (triticum aestivum l.).maturation forms one of the critical seed developmental phases and it is characterized mainly by programmed cell death, dormancy and desiccation, however, the transcriptional programs and regulatory networks underlying acquisition of dormancy and deposition of storage reserves during the maturation phase of seed development are poorly understood in wheat. the present study performed comparative spatiotemporal transcriptomic analysis of seed maturation in two wheat genotypes with contrasting seed ...201728915785
conversion of array-based single nucleotide polymorphic markers for use in targeted genotyping by sequencing in hexaploid wheat (triticum aestivum).wheat breeders and academics alike use single nucleotide polymorphisms (snps) as molecular markers to characterize regions of interest within the hexaploid wheat genome. a number of snp-based genotyping platforms are available, and their utility depends upon factors such as the available technologies, number of data points required, budgets and the technical expertise required. unfortunately, markers can rarely be exchanged between existing and newly developed platforms, meaning that previously ...201728913866
high-density snp genotyping array for hexaploid wheat and its relatives.a lack of genetic diversity between wheat breeding lines has been recognized as a significant block to future yield increases. wheat breeding and prebreeding strategies are increasingly using material from wheat ancestors or wild relatives to reintroduce diversity. where molecular markers are polymorphic between the host and introgressed material, they may be used to track the size and location of the introgressed material through generations of backcrossing. to generate markers for this purpose ...201728913809
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