Publications

characterization of the spore-forming bacillus cereus sensu lato group and clostridium perfringens bacteria isolated from the australian dairy farm environment.	the bacillus cereus sensu lato group and clostridium perfringens are spore-forming bacteria often associated with food spoilage and which can cause emetic and diarrheal syndromes in humans and ruminants. this study characterised the phenotypes and genotypes of 50 bacillus cereus s. l. isolates and 26 clostridium perfringens isolates from dairy farms environments in victoria, australia.	2015	25881096
antimicrobial evaluation of plants used for the treatment of diarrhoea in a rural community in northern maputaland, kwazulu-natal, south africa.	zulu people living in the rural area of maputaland (kwazulu-natal, south africa) rely heavily on medicinal plants for the treatment of diarrhoea. abundant availability of medicinal plants in the study area offers low cost health care, but scientific validation is needed in order to lend credibility to the traditional use against many ailments including diarrhoeal infections. with this in mind a study was designed to test the in vitro antimicrobial efficacy of 23 plant species which are used for ...	2015	25881066
characterization of cell-free extracts from fenpropathrin-degrading strain bacillus cereus zh-3 and its potential for bioremediation of pyrethroid-contaminated soils.	synthetic pyrethroid fenpropathrin has received increasing attention because of its environmental contamination and toxic effects on non-target organisms including human beings. here we report the degradation characteristics of cell-free extracts from fenpropathrin-degrading strain bacillus cereus zh-3 and its potential for pyrethroid bioremediation in soils. 50mg·l(-1) of fenpropathrin was decreased to 20.6mg·l(-1) by the enzymatic extracts (869.4mg·l(-1)) within 30min. kinetic constants km and ...	2015	25862990
purification and characterization of a novel plantaricin, kl-1y, from lactobacillus plantarum kl-1.	three bacteriocins from lactobacillus plantarum kl-1 were successfully purified using ammonium sulfate precipitation, cation-exchange chromatography and reverse-phase hplc. the bacteriocin peptides kl-1x, -1y and -1z had molecular masses of 3053.82, 3498.16 and 3533.16 da, respectively. all three peptides were stable at ph 2-12 and 25 °c and at high temperatures of 80 and 100 °c for 30 min and 121 °c for 15 min. however, they differed in their susceptibility to proteolytic enzymes and their inhi ...	2015	25862353
pulsed-field gel electrophoresis of bacillus cereus group strains.	pulsed-field gel electrophoresis (pfge) subtyping has been used extensively to characterize various bacterial species and to facilitate comparative analysis of geographically diverse populations. to this end, standardized protocols for many different genera and species have been developed, particularly through the pulsenet platform. the bacillus cereus group of bacteria includes a diverse species set, which are of particular importance in food safety as both human pathogens and spoilage organism ...	2015	25862049
spontaneous bacterial peritonitis (sbp) caused by bacillus cereus in an alcoholic patient: case report and review of literature.	spontaneous bacterial peritonitis (sbp) is infection of peritoneal covering of the abdomen caused by bacteria, without any known etiology. common known predisposing factors are cirrhosis of liver and old age among others. bacillus cereus is an uncommon cause of sbp and often wrongly interpreted as a contaminant. we hereby report a case of peritonitis in chronic alcoholic, elderly male patient presenting in the outpatient department. bacillus cereus is often regarded as contaminant but must be ca ...	2015	25859458
draft genome sequences of supercritical co2-tolerant bacteria bacillus subterraneus mitot1 and bacillus cereus mit0214.	we report draft genome sequences of bacillus subterraneus mitot1 and bacillus cereus mit0214 isolated through enrichment of samples from geologic sequestration sites in pressurized bioreactors containing a supercritical (sc) co2 headspace. their genome sequences expand the phylogenetic range of sequenced bacilli and allow characterization of molecular mechanisms of scco2 tolerance.	2015	25858826
antibiotic-resistant bacteria: prevalence in food and inactivation by food-compatible compounds and plant extracts.	foodborne antibiotic-resistant pathogenic bacteria such as campylobacter jejuni, bacillus cereus, clostridium perfringens, escherichia coli, salmonella enterica, staphylococcus aureus, vibrio cholerae, and vibrio parahemolyticus can adversely affect animal and human health, but a better understanding of the factors involved in their pathogenesis is needed. to help meet this need, this overview surveys and interprets much of our current knowledge of antibiotic (multidrug)-resistant bacteria in th ...	2015	25856120
fatty acid profiles for differentiating growth medium formulations used to culture bacillus cereus t-strain spores.	microbial biomarkers that indicate aspects of an organism's growth conditions are important targets of forensic research. in this study, we examined fatty acid composition as a signature for the types of complex nutrients in the culturing medium. bacillus cereus t-strain spores were grown in medium formulations supplemented with one of the following: peptone (meat protein), tryptone (casein protein), soy protein, and brain-heart infusion. cellular biomass was profiled with fatty acid methyl este ...	2015	25854710
temperature dependence of the proteome profile of the psychrotolerant pathogenic food spoiler bacillus weihenstephanensis type strain wsbc 10204.	bacillus weihenstephanensis is a subspecies of the bacillus cereus sensu lato group of spore-forming bacteria known to cause food spoilage or food poisoning. the key distinguishing phenotype of b. weihenstephanensis is its ability to grow below 7 °c or, from a food safety perspective, to grow and potentially produce toxins in a refrigerated environment. comparison of the proteome profile of b. weihenstephanensis upon its exposure to different culturing conditions can reveal clues to the mechanis ...	2015	25853650
organic-inorganic hybrid nanoparticles for bacterial inhibition: synthesis and characterization of doped and undoped onps with ag/au nps.	organic nanoparticles (onps) of lipoic acid and its doped derivatives onps/ag and onps/au were prepared and characterized by uv-visible, eds, and tem analysis. the antibacterial properties of the onps onps/ag and onps/au were tested against bacterial strains (staphylococcus aureus, bacillus cereus, escherichia coli and salmonella typhi). minimal inhibitory concentration (mic) and bacterial growth inhibition tests show that onps/ag are more effective in limiting bacterial growth than other nps, p ...	2015	25853317
synthesis and characterization of nanosilver with antibacterial properties using pinus densiflora young cone extract.	this study describes an eco-friendly, rapid method for green synthesis of silver nanoparticles (ag nps) from an aqueous solution of silver nitrate using pinus densiflora for. multicaulis uyeki young cone extract in a single-pot process. color changes, ultraviolet-visible spectra (444.5 nm), x-ray diffraction peaks (2θ=39.68, 46.92, 68.12, and 79.10), and fourier transform infrared spectroscopy (ft-ir) confirmed the presence of ag nps and phytochemicals. transmission electron microscopy showed th ...	2015	25846578
biodegradation of carbofuran in soils within nzoia river basin, kenya.	carbofuran (2,3-dihydro-2,2-dimethylbenzofuran-7-yl methylcarbamate) has been used within the nzoia river basin (nrb), especially in bunyala rice irrigation schemes, in kenya for the control of pests. in this study, the capacity of native bacteria to degrade carbofuran in soils from nrb was investigated. a gram positive, rod-shaped bacteria capable of degrading carbofuran was isolated through liquid cultures with carbofuran as the only carbon and nitrogen source. the isolate degraded 98% of 100- ...	2015	25844859
a rapid and miniaturized method for the selection of microbial phenol degraders using colourimetric microtitration.	a high-throughput method is described, consisting of a colourimetric microtitration for screening phenol-degrading microorganisms, using a mixture of 4-aminoantipyrine and potassium ferricyanide as the colour indicator. this contemporary study summarizes a new method to determine phenol-degrading bacteria isolated from different areas. the method was used for testing a total of 72 bacteria collected from the natural environment and five known strains obtained from diagnostic and research laborat ...	2015	25842173
microbiological diversity and prevalence of spoilage and pathogenic bacteria in commercial fermented alcoholic beverages (beer, fruit wine, refined rice wine, and yakju).	the present study examined 469 commercially available fermented alcoholic beverages (fabs), including beer (draft, microbrewed, and pasteurized), fruit wine (grape and others), refined rice wine, and yakju (raw and pasteurized). samples were screened for escherichia coli and eight foodborne pathogens (bacillus cereus, campylobacter jejuni, clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, salmonella spp., staphylococcus aureus, and yersinia enterocolitica), and the aerob ...	2015	25836410
expert elicitation as a means to attribute 28 enteric pathogens to foodborne, waterborne, animal contact, and person-to-person transmission routes in canada.	enteric illness contributes to a significant burden of illness in canada and globally. understanding its sources is a critical step in identifying and preventing health risks. expert elicitation is a powerful tool, used previously, to obtain information about enteric illness source attribution where information is difficult or expensive to obtain. thirty-one experts estimated transmission of 28 pathogens via major transmission routes (foodborne, waterborne, animal contact, person-to-person, and ...	2015	25835810
antimicrobial activities of active component isolated from lawsonia inermis leaves and structure-activity relationships of its analogues against food-borne bacteria.	the antimicrobial activities of lawsonia inermis leaf extract and 2-hydroxy-1,4-naphthoquinone analogues against food-borne bacteria. the antimicrobial activities of five fractions derived from the methanol extract of lawsonia inermis leaves were evaluated against 7 food-borne bacteria. 2-hydroxy-1,4-naphthoquinone was isolated by chromatographic analyses. 2-hydroxy-1,4-naphthoquinone showed the strong activities against bacillus cereus, listeria monocytogenes, salmonella enterica, shigella sonn ...	2015	25829631
alternative modes of biofilm formation by plant-associated bacillus cereus.	the ability to form multicellular communities known as biofilms is a widespread adaptive behavior of bacteria. members of the bacillus group of bacteria have been found to form biofilms on plant roots, where they protect against pathogens and promote growth. in the case of the model bacterium bacillus subtilis the genetic pathway controlling biofilm formation and the production of an extracellular matrix is relatively well understood. however, it is unclear whether other members of this genus ut ...	2015	25828975
structure, activity and stereoselectivity of nadph-dependent oxidoreductases catalysing the s-selective reduction of the imine substrate 2-methylpyrroline.	oxidoreductases from streptomyces sp. gf3546 [3546-ired], bacillus cereus bag3x2 (bcired) and nocardiopsis halophila (nhired) each reduce prochiral 2-methylpyrroline (2mpn) to (s)-2-methylpyrrolidine with >95 % ee and also a number of other imine substrates with good selectivity. structures of bcired and nhired have helped to identify conserved active site residues within this subgroup of imine reductases that have s selectivity towards 2mpn, including a tyrosine residue that has a possible role ...	2015	25809902
maldi-tof ms and cd spectral analysis for identification and structure prediction of a purified, novel, organic solvent stable, fibrinolytic metalloprotease from bacillus cereus b80.	the ability to predict protein function from structure is becoming increasingly important; hence, elucidation and determination of protein structure become the major steps in proteomics. the present study was undertaken for identification of metalloprotease produced by bacillus cereus b80 and recognition of characteristics that can be industrially exploited. the enzyme was purified in three steps combining precipitation and chromatographic methods resulting in 33.5% recovery with 13.1-fold purif ...	2015	25802851
the effect of selected factors on the survival of bacillus cereus in the human gastrointestinal tract.	bacillus cereus is a gram-positive bacterium widely distributed in soil and vegetation. this bacterial species can also contaminate raw or processed foods. pathogenic b. cereus strains can cause a range of infections in humans, as well as food poisoning of an emetic (intoxication) or diarrheal type (toxico-infection). toxico-infections are due to the action of the hbl toxin, nhe toxin, and cytotoxin k produced by the microorganism in the gastrointestinal tract. this occurs once the spores or veg ...	2015	25794697
determination and quantification of the emetic toxin cereulide from bacillus cereus in pasta, rice and cream with liquid chromatography-tandem mass spectrometry.	a rapid and sensitive method has been developed for determination and quantification of cereulide in cream, rice and pasta. samples are homogenised after addition of amylase to cooked rice and pasta, and cereulide is extracted with methanol. after the removal of water with methyl-tert butyl ether/hexane and evaporation until dryness, no further purification was required before analysis with liquid chromatography-tandem mass spectrometry (lc-ms/ms). recently, both cereulide and (13)c6-cereulide h ...	2015	25794364
various enterotoxin and other virulence factor genes widespread among bacillus cereus and bacillus thuringiensis strains.	many strains of bacillus cereus cause gastrointestinal diseases, and the closely related insect pathogen bacillus thuringiensis has also been involved in outbreaks of diarrhea. the diarrheal diseases are attributed to enterotoxins. sixteen reference strains of b. cereus and nine commercial and 12 reference strains of b. thuringiensis were screened by pcr for the presence of 10 enterotoxigenic genes (hbla, hblc, hbld, nhea, nheb, nhec, cytk, bcet, entfm, and ents), one emetogenic gene (ces), seve ...	2015	25791850
effect of thymol in heating and recovery media on the isothermal and non-isothermal heat resistance of bacillus spores.	members of the genus bacillus include important food-borne pathogen and spoilage microorganisms for food industry. essential oils are natural products extracted from herbs and spices, which can be used as natural preservatives in many foods because of their antibacterial, antifungal, antioxidant and anti-carcinogenic properties. the aim of this research was to explore the effect of the addition of different concentrations of thymol to the heating and recovery media on the thermal resistance of s ...	2015	25790989
grafted α-hydroxyphosphonic acids onto polymeric supports: preparation, characterization, and antimicrobial effect.	the paper deals with the preparation and characterization of compounds with antimicrobial activity: α-hydroxyphosphonic acids grafted onto styrene-12%-(15%)-divinylbenzene copolymer. these products proved to have antimicrobial effect against two species of gram-negative bacteria (escherichia coli and pseudomonas aeruginosa) and two species of gram-positive bacteria (staphylococcus aureus and bacillus cereus) and a species of yeast (candida albicans). tests showed the reduction of bacterial load ...	2015	25789417
genetic characterization, nickel tolerance, biosorption, kinetics, and uptake mechanism of a bacterium isolated from electroplating industrial effluent.	electroplating industries in madurai city produce approximately 49,000 l of wastewater and 1200 l of sludge every day revealing 687-5569 ppm of nickel (ni) with other contaminants. seventeen ni-tolerant bacterial strains were isolated from nutrient-enriched effluents. among them one hyper ni accumulating strain was scored and identified as bacillus cereus vp17 on the basis of morphology, biochemical tests, 16s rdna gene sequencing, and phylogenetic analysis. equilibrium data of ni(ii) ions using ...	2015	25768053
plant compounds enhance the assay sensitivity for detection of active bacillus cereus toxin.	bacillus cereus is an important food pathogen, producing emetic and diarrheal syndromes, the latter mediated by enterotoxins. the ability to sensitively trace and identify this active toxin is important for food safety. this study evaluated a nonradioactive, sensitive, in vitro cell-based assay, based on b. cereus toxin inhibition of green fluorescent protein (gfp) synthesis in transduced monkey kidney vero cells, combined with plant extracts or plant compounds that reduce viable count of b. cer ...	2015	25767986
lactic acid bacteria: promising supplements for enhancing the biological activities of kombucha.	kombucha is sweetened black tea that is fermented by a symbiosis of bacteria and yeast embedded within a cellulose membrane. it is considered a health drink in many countries because it is a rich source of vitamins and may have other health benefits. it has previously been reported that adding lactic acid bacteria (lactobacillus) strains to kombucha can enhance its biological functions, but in that study only lactic acid bacteria isolated from kefir grains were tested. there are many other natur ...	2015	25763303
estimation of the growth kinetic parameters of bacillus cereus spores as affected by pulsed light treatment.	quantitative microbial risk assessment requires the knowledge of the effect of food preservation technologies on the growth parameters of the survivors of the treatment. this is of special interest in the case of the new non-thermal technologies that are being investigated for minimal processing of foods. this is a study on the effect of pulsed light technology (pl) on the lag phase of bacillus cereus spores surviving the treatment and the maximum growth rate (μmax) of the survivors after germin ...	2015	25755081
bacillus halodurans strain c125 encodes and synthesizes enzymes from both known pathways to form dump directly from cytosine deoxyribonucleotides.	analysis of the genome of bacillus halodurans strain c125 indicated that two pathways leading from a cytosine deoxyribonucleotide to dump, used for dtmp synthesis, were encoded by the genome of the bacterium. the genes that were responsible, the comeb gene and the dcdb gene, encoding dcmp deaminase and the bifunctional dctp deaminase:dutpase (dcd:dut), respectively, were both shown to be expressed in b. halodurans, and both genes were subject to repression by the nucleosides thymidine and deoxyc ...	2015	25746996
characterization and in vitro biological activities of thai traditional fermented shrimp pastes.	in this work, chemical and biological characteristics of two types of thai fermented shrimp paste, kapi ta dam and kapi ta deang, at different fermentation periods and their raw materials were investigated. kapi had low water activity and high proteins with high glutamic acid and lysine. both kapis, which had different sources, showed similar characteristics. the number of lactic acid bacteria in the products increased during the early stages of fermentation. free α-amino acid contents in the pr ...	2015	25745266
incidence and enterotoxigenic profile of bacillus cereus in meat and meat products of uttarakhand, india.	the present investigation was undertaken to study the incidence and enterotoxigenicity of bacillus cereus in raw meat and meat products. b. cereus was isolated from 29 (30.9 %) of the 94 samples analyzed. recorded incidences of b. cereus from raw meat and meat products samples were 27.8 and 35 %, respectively. a high level of organism was found in cooked-meat (35 %) than raw meat samples (27.78 %) from 40 cooked-meat products and 54 raw meat samples analyzed. screening of isolates by multiplex p ...	2015	25745259
changes in physicochemical characteristics of ozone-treated raw white rice.	ozone dose from 0.1 to 0.4 ppm has been proven to be effective in lowering bacillus cereus count in uncooked and cooked rice. however, it induces physicochemical changes in raw white rice. physicochemical tests were done to see the effect of ozone treatment towards moisture content, ph, color, hardness of uncooked rice, adhesiveness and hardness of cooked rice, cooking quality and total solids. results have shown that moisture content, adhesiveness and hardness of cooked rice and uncooked rice h ...	2015	25745221
chemoenzymatic synthesis of thiazolyl peptide natural products featuring an enzyme-catalyzed formal [4 + 2] cycloaddition.	thiocillins from bacillus cereus atcc 14579 are members of the well-known thiazolyl peptide class of natural product antibiotics, the biosynthesis of which has recently been shown to proceed via post-translational modification of ribosomally encoded precursor peptides. it has long been hypothesized that the final step of thiazolyl peptide biosynthesis involves a formal [4 + 2] cycloaddition between two dehydroalanines, a unique transformation that had eluded enzymatic characterization. here we d ...	2015	25742119
adaptive responses of bacillus cereus atcc14579 cells upon exposure to acid conditions involve atpase activity to maintain their internal ph.	this study examined the involvement of atpase activity in the acid tolerance response (atr) of bacillus cereus atcc14579 strain. in the current work, b. cereus cells were grown in anaerobic chemostat culture at external ph (phe ) 7.0 or 5.5 and at a growth rate of 0.2 h(-1) . population reduction and internal ph (phi ) after acid shock at ph 4.0 was examined either with or without atpase inhibitor n,n'-dicyclohexylcarbodiimide (dccd) and ionophores valinomycin and nigericin. population reduction ...	2015	25740257
differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells.	silver nanoparticles (agnps) possess unique physical, chemical, and biological properties. agnps have been increasingly used as anticancer, antiangiogenic, and antibacterial agents for the treatment of bacterial infections in open wounds as well as in ointments, bandages, and wound dressings. the present study aimed to investigate the effects of two different sizes of agnps (10 nm and 20 nm) in male somatic leydig (tm3) and sertoli (tm4) cells and spermatogonial stem cells (sscs).	2015	25733828
influence of food matrix on outgrowth heterogeneity of heat damaged bacillus cereus spores.	spoilage of heat treated foods can be caused by the presence of surviving spore-formers. it is virtually impossible to prevent contamination at the primary production level as spores are ubiquitous present in the environment and can contaminate raw products. as a result spore inactivation treatments are widely used by food producing industries to reduce the microbial spore loads. however consumers prefer mildly processed products that have less impact on its quality and this trend steers industr ...	2015	25727186
class iv polyhydroxyalkanoate (pha) synthases and pha-producing bacillus.	this review highlights the recent investigations of class iv polyhydroxyalkanoate (pha) synthases, the newest classification of pha synthases. class iv synthases are prevalent in organisms of the bacillus genus and are composed of a catalytic subunit phac (approximately 40 kda), which has a phac box sequence ([gs]-x-c-x-[ga]-g) at the active site, and a second subunit phar (approximately 20 kda). the representative pha-producing bacillus strains are bacillus megaterium and bacillus cereus; the n ...	2015	26135986
evaluation of synergistic antibacterial and antioxidant efficacy of essential oils of spices and herbs in combination.	the present study was carried out to evaluate the possible synergistic interactions on antibacterial and antioxidant efficacy of essential oils of some selected spices and herbs [bay leaf, black pepper, coriander (seed and leaf), cumin, garlic, ginger, mustard, onion and turmeric] in combination. antibacterial combination effect was evaluated against six important food-borne bacteria (bacillus cereus, listeria monocytogenes, micrococcus luteus, staphylococcus aureus, escherichia coli and salmone ...	2015	26132146
the adaptive response of bacterial food-borne pathogens in the environment, host and food: implications for food safety.	bacteria are constantly faced to stress situations in their ecological niches, the food and the host gastrointestinal tract. the capacity to detect and respond to surrounding changes is crucial for bacterial pathogens to survive or grow in changing environments. to this purpose, cells have evolved various sophisticated networks designed to protect against stressors or repair damage caused by them. challenges can occur during production of foods when subjected to processing, and after food ingest ...	2015	26116419
from genome to toxicity: a combinatory approach highlights the complexity of enterotoxin production in bacillus cereus.	in recent years bacillus cereus has gained increasing importance as a food poisoning pathogen. it is the eponymous member of the b. cereus sensu lato group that consists of eight closely related species showing impressive diversity of their pathogenicity. the high variability of cytotoxicity and the complex regulatory network of enterotoxin expression have complicated efforts to predict the toxic potential of new b. cereus isolates. in this study, comprehensive analyses of enterotoxin gene seque ...	2015	26113843
direct recovery of cyclodextringlycosyltransferase from bacillus cereus using aqueous two-phase flotation.	purification of cyclodextrin glycosyl transferase (cgtase) from bacillus cereus using polyethylene glycol (peg)-potassium phosphates aqueous two-phase flotation (atpf) system was studied in this paper. the effects of varying peg molecular weight, tie-line length (tll) value, volume ratio (vr), ph value, crude concentration and gas nitrogen flotation time were investigated. the optimal condition for purification of cgtase was attained at 18.0% (w/w) peg 8000, 7.0% (w/w) potassium phosphates, vr o ...	2015	26111602
water-soluble moringa oleifera lectin interferes with growth, survival and cell permeability of corrosive and pathogenic bacteria.	this work evaluated the antibacterial activity of a water-soluble moringa oleifera seed lectin (wsmol) by evaluating its effect on growth, survival and cell permeability of bacillus sp., bacillus cereus, bacillus pumillus, bacillus megaterium, micrococcus sp., pseudomonas sp., pseudomonas fluorescens, pseudomonas stutzeri and serratia marcescens. in addition, the effect of lectin on membrane integrity of most sensitive species was also evaluated. all the tested bacteria are able to cause biocorr ...	2015	26109449
bacteriophages and bacteriophage-derived endolysins as potential therapeutics to combat gram-positive spore forming bacteria.	since their discovery in 1915, bacteriophages have been routinely used within eastern europe to treat a variety of bacterial infections. although initially ignored by the west due to the success of antibiotics, increasing levels and diversity of antibiotic resistance is driving a renaissance for bacteriophage-derived therapy, which is in part due to the highly specific nature of bacteriophages as well as their relative abundance. this review focuses on the bacteriophages and derived lysins of re ...	2015	26109320
antimicrobial mechanism of resveratrol-trans-dihydrodimer produced from peroxidase-catalyzed oxidation of resveratrol.	plant polyphenols are known to have varying antimicrobial potencies, including direct antibacterial activity, synergism with antibiotics and suppression of bacterial virulence. we performed the in vitro oligomerization of resveratrol catalyzed by soybean peroxidase, and the two isomers (resveratrol-trans-dihydrodimer and pallidol) produced were tested for antimicrobial activity. the resveratrol-trans-dihydrodimer displayed antimicrobial activity against the gram-positive bacteria bacillus cereus ...	2015	26109045
heme interplay between ilsa and isdc: two structurally different surface proteins from bacillus cereus.	iron is an essential element for bacterial growth and virulence. because of its limited bioavailability in the host, bacteria have adapted several strategies to acquire iron during infection. in the human opportunistic bacteria bacillus cereus, a surface protein ilsa is shown to be involved in iron acquisition from both ferritin and hemoproteins. ilsa has a modular structure consisting of a neat (near iron transporter) domain at the n-terminus, several lrr (leucine rich repeat) motifs and a slh ...	2015	26093289
study of lactic acid bacteria community from raw milk of iranian one humped camel and evaluation of their probiotic properties.	camel milk is amongst valuable food sources in iran. on the other hand, due to the presence of probiotic bacteria and bacteriocin producers in camel milk, probiotic bacteria can be isolated and identified from this food product.	2015	26060561
purification, crystallization and x-ray crystallographic studies of a bacillus cereus mepr-like transcription factor, bc0657.	transcription factors of the marr family respond to internal and external changes and regulate a variety of biological functions through ligand association with microorganisms. mepr belongs to the marr family, and its mutations are associated with the development of multidrug resistance in staphylococcus aureus, which has caused a growing health problem. in this study, a bacillus cereus mepr-like transcription regulator, bc0657, was crystallized. the bc0657 crystals diffracted to 2.05 å resoluti ...	2015	26057803
effects of dietary bacillus cereus g19, b. cereus bc-01, and paracoccus marcusii db11 supplementation on the growth, immune response, and expression of immune-related genes in coelomocytes and intestine of the sea cucumber (apostichopus japonicus selenka).	probiotics have positive effects on the nutrient digestibility and absorption, immune responses, and growth of aquatic animals, including the sea cucumber (apostichopus japonicus selenka). a 60-day feeding trial was conducted to evaluate the effects of bacillus cereus g19, b. cereus bc-01 and paracoccus marcusii db11 supplementation on the growth, immune response, and expression level of four immune-related genes (aj-p105, aj-p50, aj-rel, and aj-lys) in coelomocytes and the intestine of juvenile ...	2015	26052012
correlations between cyanobacterial density and bacterial transformation to the viable but nonculturable (vbnc) state in four freshwater water bodies.	nutrient concentrations, phytoplankton density and community composition, and the viable but nonculturable (vbnc) state of heterotrophic bacteria were investigated in three connected reservoirs and a small isolated lake in south china to study the relationship between biotic and abiotic factors and the vbnc state in bacteria. nutrient concentrations in the reservoirs increased in the direction of water flow, whereas wenshan lake was more eutrophic. cyanobacterial blooms occurred in all four wate ...	2015	26048238
production of poly-β-hydroxybutyrate by bacillus cereus ps 10 using biphasic-acid-pretreated rice straw.	poly-β-hydroxybutyrate (phb) has attracted a great deal of attention in recent years due to its potential use for production of fully degradable bioplastics, however, high cost of phb production is the major bottleneck for its wide range industrial applications. in the current study rice straw hydrolysate (rsh) was employed as a cost-effective substrate for phb production. rsh was prepared based on biphasic acid-pretreatment of rice straw i.e. first phase treatment with 1% sulphuric acid at 121 ...	2015	26047898
a novel and highly specific phage endolysin cell wall binding domain for detection of bacillus cereus.	rapid, specific and sensitive detection of pathogenic bacteria is crucial for public health and safety. bacillus cereus is harmful as it causes foodborne illness and a number of systemic and local infections. we report a novel phage endolysin cell wall-binding domain (cbd) for b. cereus and the development of a highly specific and sensitive surface plasmon resonance (spr)-based b. cereus detection method using the cbd. the newly discovered cbd from endolysin of pbc1, a b. cereus-specific bacteri ...	2015	26043681
characterization of cereulide synthetase, a toxin-producing macromolecular machine.	cereulide synthetase is a two-protein nonribosomal peptide synthetase system that produces a potent emetic toxin in virulent strains of bacillus cereus. the toxin cereulide is a depsipeptide, as it consists of alternating aminoacyl and hydroxyacyl residues. the hydroxyacyl residues are derived from keto acid substrates, which cereulide synthetase selects and stereospecifically reduces with imbedded ketoreductase domains before incorporating them into the growing depsipeptide chain. we present an ...	2015	26042597
a novel suicide plasmid for efficient gene mutation in listeria monocytogenes.	although several plasmids have been used in listeria monocytogenes for generating mutants by allelic exchange, construction of l. monocytogenes mutants has been inefficient due to lack of effective selection markers for first and second recombination events. to address this problem, we have developed a new suicide plasmid, phoss1, by using the pmad plasmid backbone and anhydrotetracycline selection marker (secy antisense rna) driven by an inducible pxyl/teto promoter. expression of the secy anti ...	2015	26038185
kinetics of bacterial inactivation by 405nm and 520nm light emitting diodes and the role of endogenous coproporphyrin on bacterial susceptibility.	photodynamic inactivation studies of microbial pathogens have focused on the use of an external photosensitizer or a precursor compound to eliminate bacteria. the present study investigated the inactivation kinetics of six bacterial pathogens by a 405nm light emitting diode (led) without the addition of any external compound. the role of endogenous coproporphyrin on the bacterial susceptibility to leds was also examined. pathogens were illuminated with leds at 25, 10 and 4°c for 9h and the inact ...	2015	26036659
estimation and evaluation of management options to control and/or reduce the risk of not complying with commercial sterility.	in a previous study, a modular process risk model, from the raw material reception to the final product storage, was built to estimate the risk of a uht-aseptic line of not complying with commercial sterility (pujol et al., 2015). this present study was focused on demonstrating how the model (updated version with uncertainty and variability separated and 2(nd) order monte carlo procedure run) could be used to assess quantitatively the influence of management options. this assessment was done in ...	2015	26032815
starch based biodegradable graft copolymer for the preparation of silver nanoparticles.	the synthesis and characterization of a novel biodegradable graft copolymer based on partially hydrolyzed polymethylacrylate (pma) grafted amylopectin (ap) was reported which was developed for the synthesis of silver nanoparticles from silver nitrate solution by facile green technique. the prepared graft copolymer was biodegradable which was shown by fungal growth. characterization of silver nanoparticles was carried out by uv-vis spectroscopy (417nm), hr-tem, saed and fesem analysis. the tem fi ...	2015	26231330
fatty acid profiles and desaturase-encoding genes are different in thermo- and psychrotolerant strains of the bacillus cereus group.	the bacillus cereus group consists of closely-related bacteria, including pathogenic or harmless strains, and whose species can be positioned along the seven phylogenetic groups of guinebretière et al. (i-vii). they exhibit different growth-temperature ranges, through thermotolerant to psychrotolerant thermotypes. among these, b. cytotoxicus is an atypical thermotolerant and food-poisoning agent affiliated to group vii whose thermotolerance contrasts with the mesophilic and psychrotolerant therm ...	2015	26227277
amplicon sequencing for the quantification of spoilage microbiota in complex foods including bacterial spores.	spoilage of food products is frequently caused by bacterial spores and lactic acid bacteria. identification of these organisms by classic cultivation methods is limited by their ability to form colonies on nutrient agar plates. in this study, we adapted and optimized 16s rrna amplicon sequencing for quantification of bacterial spores in a canned food matrix and for monitoring the outgrowth of spoilage microbiota in a ready-to-eat food matrix.	2015	26217487
lithocholic acid and derivatives: antibacterial activity.	in order to develop bioactive lithocholic acid derivatives, we prepared fifteen semi-synthetic compounds through modification at c-3 and/or c-24. the reactions showed yields ranging from 37% to 100%. the structures of all compounds obtained were identified on the basis of their spectral data (ir, ms, 1d- and 2d-nmr). the activity of lithocholic acid and derivatives was evaluated against the growth of escherichia coli, staphylococcus aureus, bacillus cereus and pseudomonas aeruginosa. the derivat ...	2015	26216208
chitinolytic bacteria-assisted conversion of squid pen and its effect on dyes and pigments adsorption.	the aim of this work was to produce chitosanase by fermenting from squid pen, and recover the fermented squid pen for dye removal by adsorption. one chitosanase induced from squid pen powder (spp)-containing medium by bacillus cereus tku034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography. the sds-page results showed its molecular mass to be around 43 kda. the tku034 chitosanase used for th ...	2015	26213948
sol-gel encapsulation of binary zn(ii) compounds in silica nanoparticles. structure-activity correlations in hybrid materials targeting zn(ii) antibacterial use.	in the emerging issue of enhanced multi-resistant properties in infectious pathogens, new nanomaterials with optimally efficient antibacterial activity and lower toxicity than other species attract considerable research interest. in an effort to develop such efficient antibacterials, we a) synthesized acid-catalyzed silica-gel matrices, b) evaluated the suitability of these matrices as potential carrier materials for controlled release of znso4 and a new zn(ii) binary complex with a suitably des ...	2015	26198972
chemical composition of nardostachys grandiflora rhizome oil from nepal--a contribution to the chemotaxonomy and bioactivity of nardostachys.	the essential oil from the dried rhizome of nardostachys grandiflora, collected from jaljale, nepal, was obtained in 1.4% yield, and a total of 72 compounds were identified constituting 93.8% of the essential oil. the rhizome essential oil of n. grandiflora was mostly composed of calarene (9.4%), valerena-4,7(11)-diene (7.1%), nardol a (6.0%), 1(10)-aristolen-9β-ol (11.6%), jatamansone (7.9%), valeranal (5.6%), and cis-valerinic acid (5.7%). the chemical composition of n. grandiflora rhizome oil ...	2015	26197553
unexpected roles for toll-like receptor 4 and trif in intraocular infection with gram-positive bacteria.	inflammation caused by infection with gram-positive bacteria is typically initiated by interactions with toll-like receptor 2 (tlr2). endophthalmitis, an infection and inflammation of the posterior segment of the eye, can lead to vision loss when initiated by a virulent microbial pathogen. endophthalmitis caused by bacillus cereus develops as acute inflammation with infiltrating neutrophils, and vision loss is potentially catastrophic. residual inflammation observed during b. cereus endophthalmi ...	2015	26195555
is cytotoxin k from bacillus cereus a bona fide enterotoxin?	cytotoxin k (cytk) produced by bacillus cereus s.l. has generally been considered to be associated with the foodborne diarrhoeal syndrome. two distinct variants of cytk have been reported: cytk-1 from bacillus cytotoxicus and cytk-2 from b. cereus. in order to determine whether cytk plays a significant role in the diarrhoeal disease, the occurrence of cytk genes was assessed among 390 b. cereus isolates with different origins including clinical and food poisoning samples and was found to be 46%. ...	2015	26186121
multiwavelength resonance raman characterization of the effect of growth phase and culture medium on bacteria.	we examine the use of multiwavelength ultraviolet (uv) resonance-raman signatures to identify the effects of growth phase and growth medium on gram-positive and gram-negative bacteria. escherichia coli (e. coli), citrobacter koseri (c. koseri), citrobacter braakii (c. braakii), and bacillus cereus (b. cereus) were grown to logarithmic and stationary phases in nutrient broth and brain heart infusion broth. resonance raman spectra of bacteria were obtained at multiple wavelengths between 220 and 2 ...	2015	26163518
stability, antimicrobial activity, and effect of nisin on the physico-chemical properties of fruit juices.	heat processing is the most commonly used hurdle for inactivating microorganisms in fruit juices. however, this preservation method could interfere with the organoleptic characteristics of the product. alternative methods have been proposed and bacteriocins such as nisin are potential candidates. however, the approval of bacteriocins as food additives is limited, especially in foods from vegetal origin. we aimed to verify the stability, the effect on physico-chemical properties, and the antimicr ...	2015	26162590
chemical composition and antioxidant and antibacterial activities of an essential oil extracted from an edible seaweed, laminaria japonica l.	laminaria japonica l. is among the most commonly consumed seaweeds in northeast asia. in the present study, l. japonica essential oil (ljeo) was extracted by microwave-hydrodistillation and analyzed by gas chromatography and mass spectroscopy. ljeo contained 21 volatile compounds, comprising 99.76% of the total volume of the essential oil, primarily tetradeconoic acid (51.75%), hexadecanoic acid (16.57%), (9z,12z)-9,12-octadecadienoic acid (12.09%), and (9z)-hexadec-9-enoic acid (9.25%). evaluat ...	2015	26147582
reducing environmental toxicity of silver nanoparticles through shape control.	the use of antibacterial silver nanomaterials in consumer products ranging from textiles to toys has given rise to concerns over their environmental toxicity. these materials, primarily nanoparticles, have been shown to be toxic to a wide range of organisms; thus methods and materials that reduce their environmental toxicity while retaining their useful antibacterial properties can potentially solve this problem. here we demonstrate that silver nanocubes display a lower toxicity toward the model ...	2015	26146787
added value of experts' knowledge to improve a quantitative microbial exposure assessment model--application to aseptic-uht food products.	in a previous study, a quantitative microbial exposure assessment (qmea) model applied to an aseptic-uht food process was developed [pujol, l., albert, i., magras, c., johnson, n. b., membré, j. m. probabilistic exposure assessment model to estimate aseptic uht product failure rate. 2015 international journal of food microbiology. 192, 124-141]. it quantified sterility failure rate (sfr) associated with bacillus cereus and geobacillus stearothermophilus per process module (nine modules in total ...	2015	26143288
complete genome sequence and phylogenetic position of the bacillus cereus group phage jbp901.	bacteriophage jbp901, isolated from fermented food, is specific for bacillus cereus group species and exhibits a broad host spectrum among a large number of b. cereus isolates. genome sequence analysis revealed a linear 159,492-bp genome with overall g+c content of 39.7 mol%, and 201 orfs. the presence of a putative methylase, the large number of trnas, and the large number of nucleotide-metabolism- and replication-related genes in jbp901 reflects its broad lytic capacity. most of the orfs showe ...	2015	26141410
application of a molecular beacon based real-time isothermal amplification (mbrtia) technology for simultaneous detection of bacillus cereus and staphylococcus aureus.	a multiplex real-time isothermal amplification assay was developed using molecular beacons for the detection of bacillus cereus and staphylococcus aureus by targeting four important virulence genes. a correlation between targeting highly accessible dna sequences and isothermal amplification based molecular beacon efficiency and sensitivity was demonstrated using phi(φ)29 dna polymerase at a constant isothermal temperature of 30 °c. it was very selective and consistently detected down to 10(1) co ...	2015	26139938
a rapid, sensitive, simple plate assay for detection of microbial alginate lyase activity.	screening of microorganisms capable of producing alginate lyase enzyme is commonly carried out by investigating their abilities to grow on alginate-containing solid media plates and occurrence of a clearance zone after flooding the plates with agents such as 10% (w/v) cetyl pyridinium chloride (cpc), which can form complexes with alginate. although the cpc method is good, advantageous, and routinely used, the agar in the media interferes with the action of cpc, which makes judgment about clearan ...	2015	26138394
identification of novel nitroreductases from bacillus cereus and their interaction with the cb1954 prodrug.	directed enzyme prodrug therapy is a form of cancer chemotherapy in which bacterial prodrug-activating enzymes, or their encoding genes, are directed to the tumour before administration of a prodrug. the prodrug can then be activated into a toxic drug at the tumour site, reducing off-target effects. the bacterial nitroreductases are a class of enzymes used in this therapeutic approach and although very promising, the low turnover rate of prodrug by the most studied nitroreductase enzyme, nfnb fr ...	2015	26415543
identification and characterization of bacillus cereus sw7-1 in bombyx mori (lepidoptera: bombycidae).	the bacterial diseases of silkworms cause significant reductions in sericulture and result in huge economic loss. this study aimed to identify and characterize a pathogen from diseased silkworm. sw7-1, a pathogenic bacterial strain, was isolated from the diseased silkworm. the strain was identified on the basis of its bacteriological properties and 16s rrna gene sequence. the colony was round, slightly convex, opaque, dry, and milky on a nutrient agar medium, the colony also exhibited jagged edg ...	2015	26411789
composition, in vitro cytotoxic, and antimicrobial activities of the flower essential oil of diospyros discolor from taiwan.	this study investigated the chemical composition, in vitro cytotoxic, and antimicrobial activities of the essential oil isolated from the flower of diospyros discolor from taiwan. the essential oil was isolated using hydrodistillation in a clevenger-type apparatus, and characterized by gc-fid and gc-ms. twenty-eight compounds were identified, representing 100% of the oil. the main components identified were (2z,6e)-farnesol (35.0%), α-cadinol (10.9%), (e)-nerolidol (6.9%), α-humulene (6.0%), τ-c ...	2015	26411038
isolation and characterization of bacteriocinogenic lactic bacteria from m-tuba and tepache, two traditional fermented beverages in méxico.	mexican tuba (m-tuba) and tepache are mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. at present, reports on the microbiota and nutritional effects of both beverages are lacking. the purpose of this study was to determine whether m-tuba and tepache contain cultivable lactic acid bacteria (lab) capable of producing bacteriocins. tepache and m-tuba contain mesophilic aerobic bacteria, lab, and yeast. bacillus subtilis, listeria monocytogenes, listeri ...	2015	26405529
antibacterial effect and mechanism of high-intensity 405 ± 5 nm light emitting diode on bacillus cereus, listeria monocytogenes, and staphylococcus aureus under refrigerated condition.	this study investigated the antibacterial effect of 405 ± 5 nm light emitting diode (led) on bacillus cereus, listeria monocytogenes, and staphylococcus aureus, and examined its antibacterial mechanism by determining the bacterial membrane and dna damages. a 405 ± 5 nm led illuminated the gram-positive pathogens until 486 j/cm(2) at 4 °c. weibull model was used to calculate reliable life (tr) to compare bacterial sensitivities to led illumination. the membrane damage was determined by nacl and l ...	2015	26398810
health-promoting value and food applications of black cumin essential oil: an overview.	black cumin (nigella sativa l.) seeds and its essential oil have been widely used in functional foods, nutraceuticals and pharmaceutical products. analysis of nigella sativa essential oil using gc and gc-ms resulted in the identification of many bioactive compounds representing ca. 85 % of the total content. the main compounds included p-cymene, thymoquinone, α-thujene, longifolene, β-pinene, α-pinene and carvacrol. nigella sativa essential oil exhibited different biological activities including ...	2015	26396361
complete genome sequence of bacillus thuringiensis strain hd521.	bacillus thuringiensis is the most widely used biological pesticide in the world. it belongs to the bacillus cereus sensu lato group, which contains six species. among these six species, b. thuringiensis, b. anthracis, and b. cereus have a low genetic diversity. b. thuringiensis strain hd521 shows maroon colony which is different from most of the b. thuringiensis strains. strain hd521 also displays an ability to inhibit plant sheath blight disease pathogen (rhizoctonia solani ag1 ib) growth and ...	2015	26380647
genomic insights into the taxonomic status of the bacillus cereus group.	the identification and phylogenetic relationships of bacteria within the bacillus cereus group are controversial. this study aimed at determining the taxonomic affiliations of these strains using the whole-genome sequence-based genome blast distance phylogeny (gbdp) approach. the gbdp analysis clearly separated 224 strains into 30 clusters, representing eleven known, partially merged species and accordingly 19-20 putative novel species. additionally, 16s rrna gene analysis, a novel variant of mu ...	2015	26373441
structural and computational analysis of peptide recognition mechanism of class-c type penicillin binding protein, alkaline d-peptidase from bacillus cereus df4-b.	alkaline d-peptidase from bacillus cereus df4-b, called adp, is a d-stereospecific endopeptidase reacting with oligopeptides containing d-phenylalanine (d-phe) at n-terminal penultimate residue. adp has attracted increasing attention because it is useful as a catalyst for synthesis of d-phe oligopeptides or, with the help of substrate mimetics, l-amino acid peptides and proteins. structure and functional analysis of adp is expected to elucidate molecular mechanism of adp. in this study, the crys ...	2015	26370172
clinical characteristics and antimicrobial susceptibility of bacillus cereus blood stream infections.	bacillus cereus is one of the pathogens causing nosocomial bloodstream infections (bsis). however, few reports have documented the antimicrobial susceptibility and clinical characteristics of bacillus cereus bsi and the importance of empirical therapy. the aim of this study was to investigate the clinical characteristics and antimicrobial susceptibility of b. cereus isolates from patients with bsi and to analyze the impact of appropriate empirical therapy on the outcome of patients with b. cereu ...	2015	26370137
high-rate biological denitrification in the cyclic rotating-bed biological reactor: effect of cod/no3(-), nitrate concentration and salinity and the phylogenetic analysis of denitrifiers.	the effects of cod/no3(-) ratio, nitrate concentration and salinity was tested on the performance of the crbr in denitrification with catechol as carbon source. the maximum nitrate reduction attained at cod/no3(-) ratio of 1. the crbr operated at optimum cod/no3(-) ratio could completely denitrify the nitrate at inlet concentration up to 1250mg/l without nitrite accumulation. the maximum denitrification rate in the crbr was 3.56kgno3(-)/m(3)d with a nitrate reduction efficiency of 99% when the b ...	2015	26369277
merosesquiterpenoids and ten-membered macrolides from a soft coral-derived lophiostoma sp. fungus.	one new merosesquiterpenoid, craterellin d (1), along with one known analog, craterellin a (2), and five known ten-membered macrolides, 3-7, were isolated from a soft coral-derived lophiostoma sp. fungus. the absolute configuration of 1 was established based on biogenetic consideration with the co-isolated analog 2, whose configuration was determined by modified mosher's method and single-crystal x-ray diffraction analysis using cukα radiation. the absolute configuration of 3 was determined by x ...	2015	26363884
essential oils and diethyl ether extracts of serbian xeranthemum cylindraceum and x. annum: chemical composition, antimicrobial activity, and chemotaxonomic implications.	detailed gc and gc-ms analyses of the essential oils and et2 o extracts of two xeranthemum species - x. cylindraceum and x. annum - resulted in the identification of 254 components, in total. terpenoids constituted the major part of both x. cylindraceum and x. annum essential oils and extracts (51.8-65.7%, and 50.7%, resp.). among the sesquiterpenoids, the extracts of both investigated taxa contained the guaianolide xerantholide, its 11,13-dihydro derivatives, and two additional sesquiterpene la ...	2015	26363882
multiparametric quantitation of the bacillus cereus toxins cereulide and isocereulides a-g in foods.	consumption of food products contaminated with cereulide (1), a toxin produced by bacillus cereus, might cause intoxications with symptoms reported to range from indigestion pain and emesis to death. recently, a series of structural variants, coined isocereulides a-g (2-8), were identified for the first time to be produced along with cereulide (1). the observation that isocereulide a (2) shows an ∼ 8-fold increased cytotoxicity when compared to 1 urges the development of analytical tools enablin ...	2015	26352912
chemical composition and in vitro antibacterial activity of mentha spicata essential oil against common food-borne pathogenic bacteria.	the aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of mentha spicata plant against common food-borne pathogenic bacteria (staphylococcus aureus, bacillus subtilis, bacillus cereus, listeria monocytogenes, salmonella typhimurium, and escherichia coli o157:h7). chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (gc-ms). the antibacterial activity of the es ...	2015	26351584
reduction of listeria monocytogenes and bacillus cereus in milk by zinc oxide nanoparticles.	direct addition of antimicrobial materials to food during food processing is an effective method for controlling microbial contaminants of food and extending the shelf- life of food products. objective of this research was to study the antimicrobial effect of zinc oxide (zno) nanoparticle and potential applications of zno nanoparticles in terms of controling two food-borne pathogens in milk.	2015	26351469
a study on characterization of new bacteriocin produced from a novel strain of lactobacillus spicheri g2 isolated from gundruk- a fermented vegetable product of north east india: a novel bacteriocin production from lactobacillus spicheri g2.	bacteriocin producing lactobacillus spicheri g2, isolated from gundruk - a traditional fermented vegetable product of north east india. l. spicheri g2 identified by morphological, biochemical techniques followed by 16s rrna gene technique. the 16sr rna sequence of bacteriocin producer is registered in ncbi under accession no. jx481912. the bacteriocin producing potential of l. spicheri is being reported for the first time in the present investigation. bacteriocin of l. spicheri g2 showed strong ...	2015	26344995
poly(n-isopropylacrylamide)-gated fe3o4/sio2 core shell nanoparticles with expanded mesoporous structures for the temperature triggered release of lysozyme.	core-shell nanoparticles comprised of fe3o4 cores and a mesoporous silica shell with an average expanded pore size of 6.07 nm and coated with a poly(n-isopropylacrylamide) (pnipam) layer (cs-msns-ep-pnipam) were prepared and characterized. the nanoparticles was loaded with (ru(bipy)3(2+)) dye or an antibacterial enzyme, lysozyme, to obtain cs-msns-ep-pnipam-ru(bipy)3(2+) and cs-msns-ep-pnipam-lys, respectively. the lysozyme loading was determined to be 160 mg/g of nanoparticle. it was seen that ...	2015	26335056
inhibition of quorum sensing-mediated biofilm formation in pseudomonas aeruginosa by a locally isolated bacillus cereus.	quorum sensing has been shown to play a crucial role in pseudomonas aeruginosa pathogenesis where it activates expression of myriad genes that regulate the production of important virulence factors such as biofilm formation. antagonism of quorum sensing is an excellent target for antimicrobial therapy and represents a novel approach to combat drug resistance. in this study, chromobacterium violaceum biosensor strain was employed as a fast, sensitive, reliable, and easy to use tool for rapid scre ...	2015	26288125
toxin profile, biofilm formation, and molecular characterization of emetic toxin-producing bacillus cereus group isolates from human stools.	emetic toxin-producing bacillus cereus group species are an important problem, because the staple food for korean is grains such as rice. in this study, we determined the prevalence (24 of 129 isolates) of emetic b. cereus in 36,745 stool samples from sporadic food-poisoning cases in korea between 2007 and 2008. the toxin gene profile, toxin production, and biofilm-forming ability of the emetic b. cereus isolates were investigated. repetitive element sequence polymorphism polymerase chain reacti ...	2015	26287636
antibacterial effects and action modes of asiatic acid.	in this study, the antibacterial effects and action modes of asiatic acid against the foodborne bacterial pathogens escherichia coli o157:h7, salmonella typhimurium dt104, pseudomonas aeruginosa, listeria monocytogenes, staphylococcus aureus, enterococcus faecalis, and bacillus cereus were examined. minimal inhibitory concentrations (mics) of asiatic acid against these bacteria were in the range of 20-40 μg/ml. minimum bactericidal concentrations of asiatic acid were in the range of 32-52 μg/ml. ...	2015	26280399
comparison of oxytetracycline degradation behavior in pig manure with different antibiotic addition methods.	using manure collected from swine fed with diet containing antibiotics and antibiotic-free swine manure spiked with antibiotics are the two common methods of studying the degradation behavior of veterinary antibiotic in manure in the environment. however, few studies had been conducted to co-compare these two different antibiotic addition methods. this study used oxytetracycline (otc) as a model antibiotic to study antibiotic degradation behavior in manure under the above two otc addition method ...	2015	26278905
bacillus cereus cerebral abscess during induction chemotherapy for childhood acute leukemia.	a 5-year-old boy with standard-risk b-cell acute lymphoblastic anemia developed fever during induction chemotherapy. the patient had no neurological symptoms. blood cultures grew bacillus cereus and neuroimaging studies demonstrated a cerebral abscess. imaging changes resolved after completion of antibiotics. bacillus cereus bacteremia is increasingly implicated as the cause of life-threatening infections, including cerebral abscesses, in compromised patients. positive blood cultures for this or ...	2015	26274034
a type iii protein-rna toxin-antitoxin system from bacillus thuringiensis promotes plasmid retention during spore development.	members of the bacillus cereus sensu lato group of bacteria often contain multiple large plasmids, including those encoding virulence factors in b. anthracis. bacillus species can develop into spores in response to stress. during sporulation the genomic content of the cell is heavily compressed, which could result in counterselection of extrachromosomal genomic elements, unless they have robust stabilization and segregation systems. toxin-antitoxin (ta) systems are near-ubiquitous in prokaryotes ...	2015	26274022
isolation and characterisation of non-anaerobic butanol-producing symbiotic system tsh06.	butanol-producing microorganisms are all obligate anaerobes. in this study, a unique symbiotic system tsh06 was isolated to be capable of producing butanol under non-anaerobic condition. denaturing gradient gel electrophoresis (dgge) analysis of 16s ribosomal rna (rrna) revealed that two strains coexist in tsh06. the two strains were identical to clostridium acetobutylicum and bacillus cereus, respectively. they were isolated individually and named as c. acetobutylicum tsh1 and b. cereus tsh2. c ...	2015	26272091
epidemiologic investigation of a cluster of neuroinvasive bacillus cereus infections in 5 patients with acute myelogenous leukemia.	background. five neuroinvasive bacillus cereus infections (4 fatal) occurred in hospitalized patients with acute myelogenous leukemia (aml) during a 9-month period, prompting an investigation by infection control and public health officials. methods. medical records of case-patients were reviewed and a matched case-control study was performed. infection control practices were observed. multiple environmental, food, and medication samples common to aml patients were cultured. multilocus sequenc ...	2015	26269794
production and characterization of poly-3-hydroxybutyrate from bacillus cereus ps 10.	usage of renewable raw materials for production of fully degradable bioplastics (bacterial poly-3-hydroxybutyrate, phb) has gained immense research impetus considering recalcitrant nature of petroleum based plastics, dwindling fossil fuel feed stocks, and associated green house gas emissions. however, high production cost of phb is the major bottleneck for its wide range industrial applications. in current study, bacillus cereus ps 10, a recent isolate, efficiently utilized molasses, an abundant ...	2015	26257381