Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| carf (collaborator of arf) interacts with hdm2: evidence for a novel regulatory feedback regulation of carf-p53-hdm2-p21waf1 pathway. | we initially cloned carf (collaborator of arf), as a novel arf-binding protein by a yeast interaction screen. it also interacts with p53 directly leading to arf-independent enhancement of p53 function and in turn undergoes a negative feedback regulation. herein we report that i) carf interacts with hdm2 and undergoes degradation by an hdm2-dependent proteasome pathway, and ii) it acts as a transcriptional repressor of hdm2. by overexpression and silencing studies, we demonstrated that carf exert ... | 2008 | 18292944 |
| transcriptional regulation of phosphatidylcholine biosynthesis. | phosphatidylcholine biosynthesis in animal cells is primarily regulated by the rapid translocation of ctp:phosphocholine cytidylyltransferase alpha between a soluble form that is inactive and a membrane-associated form that is activated. until less than 10 years ago there was no information on the transcriptional regulation of phosphatidylcholine biosynthesis. research has identified the transcription factors sp1, rb, tef4, ets-1 and e2f as enhancing the expression of the cytidylyltransferase an ... | 2008 | 18295604 |
| engineering of mucin-type human glycoproteins in yeast cells. | mucin-type o-glycans are the most typical o-glycans found in mammalian cells and assume many different biological roles. here, we report a genetic engineered yeast strain capable of producing mucin-type sugar chains. genes encoding bacillus subtilis udp-gal/galnac 4-epimerase, human udp-gal/galnac transporter, human ppgalnac-t1, and drosophila melanogaster core1 beta1-3 galt were introduced into saccharomyces cerevisiae. the engineered yeast was able to produce a muc1a peptide containing o-glyca ... | 2008 | 18296643 |
| comparison of induced and cancer-associated mutational spectra using multivariate data analysis. | one of the most useful tools for investigating the aetiopathology of cancer is the mutation spectrum, which comprises the type and distribution of mutations within a gene sequence. many studies have generated mutagen-induced spectra using in vitro or in vivo model systems in an attempt to find correlations with those observed in cancer-associated genes such as the tp53 tumour suppressor gene. consequently, meaningful similarities in the types of mutation found in induced and human spectra have b ... | 2008 | 18296683 |
| the evolution of combinatorial gene regulation in fungi. | it is widely suspected that gene regulatory networks are highly plastic. the rapid turnover of transcription factor binding sites has been predicted on theoretical grounds and has been experimentally demonstrated in closely related species. we combined experimental approaches with comparative genomics to focus on the role of combinatorial control in the evolution of a large transcriptional circuit in the fungal lineage. our study centers on mcm1, a transcriptional regulator that, in combination ... | 2008 | 18303948 |
| first isolation of the anamorphic basidiomycetous yeast trichosporon faecale in germany, from the skin of a patient with tinea pedis. | mycoses due to yeasts belonging to other genera than candida have become common in the last years especially in immuno-compromised patients. species of the anamorphic basidiomycetous yeast genus trichosporon are such opportunistic human pathogenic yeasts which cause several diseases. in this study, trichosporon faecale is reported in germany for the first time. the isolate was taken from a human foot, where it was associated with a tinea pedis. the fungal isolate was identified by investigating ... | 2008 | 18306057 |
| integral assessment of estrogenic potentials of sediment-associated samples. part 1: the influence of salinity on the in vitro tests elra, e-screen and yes. | exogenic endocrine-active substances are also called 'endocrine disrupting chemicals' (edc). they imitate or hinder the function of natural endogenic hormones or disturb the synthesis or the metabolism of hormones or of hormone receptors. the enzyme-linked receptor assay (elra) can detect estrogenic and anti-estrogenic effects at the level of receptor binding and is a useful tool for the integrative detection of contaminant effects. although the test system has been used repeatedly in sediment a ... | 2008 | 18306891 |
| a cancer-associated ring finger protein, rnf43, is a ubiquitin ligase that interacts with a nuclear protein, hap95. | rnf43 is a recently discovered ring finger protein that is implicated in colon cancer pathogenesis. this protein possesses growth-promoting activity but its mechanism remains unknown. in this study, to gain insight into the biological action of rnf43 we characterized it biochemically and intracellularly. a combination of indirect immunofluorescence analysis and biochemical fractionation experiments suggests that rnf43 resides in the endoplasmic reticulum (er) as well as in the nuclear envelope. ... | 2008 | 18313049 |
| two distinct structural elements of 5s rrna are needed for its import into human mitochondria. | rna import into mitochondria is a widespread phenomenon. studied in details for yeast, protists, and plants, it still awaits thorough investigation for human cells, in which the nuclear dna-encoded 5s rrna is imported. only the general requirements for this pathway have been described, whereas specific protein factors needed for 5s rrna delivery into mitochondria and its structural determinants of import remain unknown. in this study, a systematic analysis of the possible role of human 5s rrna s ... | 2008 | 18314502 |
| hepatitis b virus x protein enhances nfkappab activity through cooperating with vbp1. | hepatitis b virus x protein (hbx) is essential for hepatitis b virus infection and exerts a pleiotropic effect on various cellular machineries. hbx has been also demonstrated as an indirect transcriptional transactivator of various different viral and cellular promoters. in addition, hbx is involved in the development of various liver diseases including hepatocellular carcinoma. however the mechanism of hbx in hepatocellular carcinogenesis remains largely unknown. in this study, to identify poss ... | 2008 | 18315953 |
| human hltf functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination. | human helicase-like transcription factor (hltf) is frequently inactivated in colorectal and gastric cancers. here, we show that hltf is a functional homologue of yeast rad5 that promotes error-free replication through dna lesions. hltf and rad5 share the same unique structural features, including a ring domain embedded within a swi/snf helicase domain and an hiran domain. we find that inactivation of hltf renders human cells sensitive to uv and other dna-damaging agents and that hltf complements ... | 2008 | 18316726 |
| the inheritance of mtdna in lager brewing strains. | in this work, we compared the mtdna of a number of interspecific saccharomyces hybrids (saccharomyces cerevisiae x saccharomyces uvarum and s. cerevisiae x saccharomyces bayanus) to the mtdna of 22 lager brewing strains that are thought to be the result of a natural hybridization between s. cerevisiae and another saccharomyces yeast, possibly belonging to the species s. bayanus. we detected that in hybrids constructed in vitro, the mtdna could be inherited from either parental strain. conversely ... | 2008 | 18318709 |
| control of the ability of profilin to bind and facilitate nucleotide exchange from g-actin. | a major factor in profilin regulation of actin cytoskeletal dynamics is its facilitation of g-actin nucleotide exchange. however, the mechanism of this facilitation is unknown. we studied the interaction of yeast (ypf) and human profilin 1 (hpf1) with yeast and mammalian skeletal muscle actins. homologous pairs (ypf and yeast actin, hpf1 and muscle actin) bound more tightly to one another than heterologous pairs. however, with saturating profilin, hpf1 caused a faster etheno-atp exchange with bo ... | 2008 | 18223293 |
| genome-wide survey and developmental expression mapping of zebrafish set domain-containing genes. | set domain-containing proteins represent an evolutionarily conserved family of epigenetic regulators, which are responsible for most histone lysine methylation. since some of these genes have been revealed to be essential for embryonic development, we propose that the zebrafish, a vertebrate model organism possessing many advantages for developmental studies, can be utilized to study the biological functions of these genes and the related epigenetic mechanisms during early development. to this e ... | 2008 | 18231586 |
| reconstitution of mdm2-dependent post-translational modifications of p53 in yeast. | p53 mediates cell cycle arrest or apoptosis in response to dna damage. its activity is subject to a tight regulation involving a multitude of post-translational modifications. the plethora of functional protein interactions of p53 at present precludes a clear understanding of regulatory principles in the p53 signaling network. to circumvent this complexity, we studied here the minimal requirements for functionally relevant p53 post-translational modifications by expressing human p53 together wit ... | 2008 | 18231594 |
| high mobility group box (hmgb) proteins of plasmodium falciparum: dna binding proteins with pro-inflammatory activity. | high mobility group box chromosomal protein 1 (hmgb1), known as an abundant, non-histone architectural chromosomal protein, is highly conserved across different species. homologues of hmgb1 were identified and cloned from malaria parasite, plasmodium falciparum. sequence analyses showed that the p. falciparum hmgb1 (pfhmgb1) exhibits 45, 23 and 18%, while pfhmgb2 shares 42, 21 and 17% homology with saccharomyces cerevisiae, human and mouse hmg box proteins respectively. parasite pfhmgb1and pfhmg ... | 2008 | 18234548 |
| a functional link between the human cell cycle-regulatory phosphatase cdc14a and the atypical mitogen-activated kinase erk3. | cdc14 is a member of the dual-specificity phosphatase family, which is essential for faithful cell cycle progression in eukaryotic cells of different origin. the function of human cdc14a (hcdc14a), however, has not been fully elucidated as only few physiological substrates have been identified. to gain insight into the biological role of cdc14a, we performed a yeast two-hybrid screen designed to isolate substrates of this human phosphatase. using this genetic approach, we here report the identif ... | 2008 | 18235225 |
| dbc1 is a negative regulator of sirt1. | the nad-dependent protein deacetylase sir2 (silent information regulator 2) regulates lifespan in several organisms. sirt1, the mammalian orthologue of yeast sir2, participates in various cellular functions and possibly tumorigenesis. whereas the cellular functions of sirt1 have been extensively investigated, less is known about the regulation of sirt1 activity. here we show that deleted in breast cancer-1 (dbc1), initially cloned from a region (8p21) homozygously deleted in breast cancers, form ... | 2008 | 18235501 |
| interactions of no with hemoglobin: from microbes to man. | hemoglobins are found in organisms from every major phylum and subserve life-sustaining respiratory functions across a broad continuum. sustainable aerobic respiration in mammals and birds relies on the regulated delivery of oxygen (o2) and nitric oxide (no) bioactivity by hemoglobin, through reversible binding of no and o2 to hemes as well as s-nitrosylation of cysteine thiols (sno synthase activity). in contrast, bacterial and yeast flavohemoglobins function in vivo as denitrosylases (o2 nitro ... | 2008 | 18237631 |
| devices for home evaluation of women's health concerns. | devices used for home evaluation of fertility, pregnancy, menopause, colon cancer, breast cancer, and urinary-tract and vaginal yeast infections are discussed. | 2008 | 18238767 |
| acetaldehyde-induced mutational pattern in the tumour suppressor gene tp53 analysed by use of a functional assay, the fasay (functional analysis of separated alleles in yeast). | chronic alcohol consumption is a major risk factor for upper aero-digestive tract cancers, including cancer of the esophagus. whereas alcohol as such is not thought to be directly carcinogenic, acetaldehyde, its first metabolite, has been proven genotoxic and mutagenic in the hprt gene. as mutations in the tumour suppressor gene tp53 are the most common genetic alterations involved in human cancers, especially esophageal tumours, the aim of this work was to establish the mutational pattern induc ... | 2008 | 18242117 |
| role of homologous recombination in trabectedin-induced dna damage. | trabectedin (et-743, yondelis) is a natural marine compound with antitumour activity currently undergoing phase ii/iii clinical trials. the mechanism of the drug's action is still to be defined, even though it has been clearly demonstrated the key role of nucleotide excision repair (ner). to get further insights into the drug's mode of action, we studied the involvement of the dna-double strand break repair (dna-dsb) pathways: homologous and non-homologous recombination, both in budding yeasts a ... | 2008 | 18243687 |
| centromere anatomy in the multidrug-resistant pathogen enterococcus faecium. | multidrug-resistant variants of the opportunistic human pathogen enterococcus have recently emerged as leading agents of nosocomial infection. the acquisition of plasmid-borne resistance genes is a driving force in antibiotic-resistance evolution in enterococci. the segregation locus of a high-level gentamicin-resistance plasmid, pgent, in enterococcus faecium was identified and dissected. this locus includes overlapping genes encoding prgp, a member of the para superfamily of segregation protei ... | 2008 | 18245388 |
| genotoxicity of tryptophol in a battery of short-term assays on human white blood cells in vitro. | the genotoxic effects of tryptophol (indole-3-ethanol), an aromatic alcohol and known secondary metabolite of the opportunistic yeast candida albicans and other candida spp., were studied using a battery of short-term assays on human white blood cells in vitro. the concentration range of tryptophol tested was 0.25 mm to 2.00 mm. lymphocyte viability and induction of apoptosis/necrosis were studied by simultaneous use of a fluorescent assay with ethidium bromide and acridine orange. levels of pri ... | 2008 | 18248512 |
| fld1p, a functional homologue of human seipin, regulates the size of lipid droplets in yeast. | lipid droplets (lds) are emerging cellular organelles that are of crucial importance in cell biology and human diseases. in this study, we present our screen of approximately 4,700 saccharomyces cerevisiae mutants for abnormalities in the number and morphology of lds; we identify 17 fld (few lds) and 116 mld (many lds) mutants. one of the fld mutants (fld1) is caused by the deletion of ylr404w, a previously uncharacterized open reading frame. cells lacking fld1 contain strikingly enlarged (super ... | 2008 | 18250201 |
| role for plk1 phosphorylation of hbo1 in regulation of replication licensing. | in a search for polo-like kinase 1 (plk1)-interacting proteins using a yeast two-hybrid system, we have identified histone acetyltransferase binding to the origin recognition complex 1 (hbo1) as a potential plk1 target. here, we show that the interaction between plk1 and hbo1 is mitosis-specific and that plk1 phosphorylates hbo1 on ser-57 in vitro and in vivo. during mitosis, cdk1 phosphorylates hbo1 on thr-85/88, creating a docking site for plk1 to be recruited. significantly, the overexpressio ... | 2008 | 18250300 |
| a novel mammalian trans-membrane protein reveals an alternative initiation pathway for autophagy. | autophagy is an early cellular event during acute pancreatitis, a disease defined as pancreas self-digestion. the vacuole membrane protein 1 (vmp1) is a trans-membrane protein highly activated in acinar cells early during pancreatitis-induced autophagy and it remains in the autophagosomal membrane. we have shown that vmp1 expression is able to trigger autophagy in mammalian cells, even under nutrient-replete conditions. vmp1 is induced by autophagy stimuli and its expression is required for auto ... | 2008 | 18253086 |
| rna and transcriptional modulation of gene expression. | the roles that rna molecules play in the regulation of gene expression have only recently begun to come to light. recent work in this area has uncovered several complex, rna-mediated networks of gene regulation in eukaryotic systems. one newly discovered mechanism of rna mediated gene regulation takes place at the level of transcription. in yeast, plant, and mammalian systems, small rnas targeted to gene promoters can result in a repression of transcription. small rna mediated transcriptional si ... | 2008 | 18256543 |
| reversal of aging by nfkappab blockade. | genetic studies in model organisms such as yeast, worms, flies, and mice leading to lifespan extension suggest that longevity is subject to regulation. in addition, various system-wide interventions in old animals can reverse features of aging. to better understand these processes, much effort has been put into the study of aging on a molecular level. in particular, genome-wide microarray analysis of differently aged individual organisms or tissues has been used to track the global expression ch ... | 2008 | 18256548 |
| the importance of conserved amino acid residues in p94 protease sub-domain iib and the is2 region for constitutive autolysis. | p94/calpain 3, a skeletal muscle-specific member of calpain protease family, is characterized by apparent ca(2+)-independence during exhaustive autolysis and concomitant proteolysis of non-self substrates. the purpose of our study was to comprehensively profile the structural basis of p94 enabling activation in the cytosol without an extra ca(2+). ca(2+)-dependent p94 mutants were screened using "p94-trapping", which is an application of yeast genetic reporter system called "proteinase-trapping" ... | 2008 | 18258189 |
| pi3k signaling of autophagy is required for starvation tolerance and virulenceof cryptococcus neoformans. | autophagy is a process by which cells recycle cytoplasm and defective organelles during stress situations such as nutrient starvation. it can also be used by host cells as an immune defense mechanism to eliminate infectious pathogens. here we describe the use of autophagy as a survival mechanism and virulence-associated trait by the human fungal pathogen cryptococcus neoformans. we report that a mutant form of c. neoformans lacking the vps34 pi3k (vps34delta), which is known to be involved in au ... | 2008 | 18259613 |
| architecture of transcriptional regulatory circuits is knitted over the topology of bio-molecular interaction networks. | uncovering the operating principles underlying cellular processes by using 'omics' data is often a difficult task due to the high-dimensionality of the solution space that spans all interactions among the bio-molecules under consideration. a rational way to overcome this problem is to use the topology of bio-molecular interaction networks in order to constrain the solution space. such approaches systematically integrate the existing biological knowledge with the 'omics' data. | 2008 | 18261202 |
| nuclear magnetic resonance spectroscopy and genetic disorders. | nuclear magnetic resonance spectroscopy has been exploited to study the metabolic characteristics (phenotype) of genetic disorders by taking advantage of some unique characteristics of the technique. the first application, metabolic profiling for diagnosis and therapeutic monitoring in vitro, demonstrates the exceptional diversity of metabolites detected by nmr, and has resulted in new interest in significant metabolites largely ignored previously because other techniques do not detect them, e.g ... | 2008 | 18220760 |
| yeast of the oral cavity is the reservoir of heliobacter pylori. | frequent occurrence of helicobacter pylori in the human gastrointestinal tract and its persistence due to unsuccessful antimicrobial therapy might be related to a stage in the life cycle of h. pylori in which the bacterium establishes itself as an intracellular symbiont in yeast. in this study, occurrence of non-culturable h. pylori in the oral yeast was assessed by targeting vacuolating cytotoxin a (vaca s1s2) and ureab genes in the total dnas of yeasts. | 2008 | 18266659 |
| hubba: hub objects analyzer--a framework of interactome hubs identification for network biology. | one major task in the post-genome era is to reconstruct proteomic and genomic interacting networks using high-throughput experiment data. to identify essential nodes/hubs in these interactomes is a way to decipher the critical keys inside biochemical pathways or complex networks. these essential nodes/hubs may serve as potential drug-targets for developing novel therapy of human diseases, such as cancer or infectious disease caused by emerging pathogens. hub objects analyzer (hubba) is a web-bas ... | 2008 | 18503085 |
| schizosaccharomyces pombe histone acetyltransferase mst1 (kat5) is an essential protein required for damage response and chromosome segregation. | schizosaccharomyces pombe mst1 is a member of the myst family of histone acetyltransferases and is the likely ortholog of saccharomyces cerevisiae esa1 and human tip60 (kat5). we have isolated a temperature-sensitive allele of this essential gene. mst1 cells show a pleiotropic phenotype at the restrictive temperature. they are sensitive to a variety of dna-damaging agents and to the spindle poison thiabendazole. mst1 has an increased frequency of rad22 repair foci, suggesting endogenous damage. ... | 2008 | 18505873 |
| anti-beta-glucan-like immunoprotective candidacidal antiidiotypic antibodies. | mycoses, candidiasis in particular, are relatively common opportunistic infections still characterized by an unacceptable high mortality rate. furthermore, they are often complicated by resistance or refractoriness to the existing antimicrobial agents. in recent years new effective therapeutic and large-scale preventative strategies have been proposed by exploiting the identification of fungal beta-glucans as target of antifungal agents such as echinocandins, yeast killer toxins and protective a ... | 2008 | 18508705 |
| topbp1 activates atr through atrip and a pikk regulatory domain. | the atr (atm and rad3-related) kinase and its regulatory partner atrip (atr-interacting protein) coordinate checkpoint responses to dna damage and replication stress. topbp1 functions as a general activator of atr. however, the mechanism by which topbp1 activates atr is unknown. here, we show that atrip contains a topbp1-interacting region that is necessary for the association of topbp1 and atr, for topbp1-mediated activation of atr, and for cells to survive and recover dna synthesis following r ... | 2008 | 18519640 |
| immunoassays: biological tools for high throughput screening and characterisation of combinatorial libraries. | in the demanding field of proteomics, there is an urgent need for affinity-catcher molecules to implement effective and high throughput methods for analysing the human proteome or parts of it. antibodies have an essential role in this endeavour, and selection, isolation and characterisation of specific antibodies represent a key issue to meet success. alternatively, it is expected that new, well-characterised affinity reagents generated in rapid and cost-effective manners will also be used to fa ... | 2008 | 18473742 |
| live yeast cell derivative induces c-fos expression in thp-1 monocytes. | live yeast cell derivative is a medicinal extract of saccharomyces cerevisiae that has demonstrated efficacy in improving the rate and quality of wound healing in mouse and human systems. however, the mechanisms by which lycd promotes healing are largely uncharacterized. in this report, we demonstrate that lycd has effects on the transcriptional profile of the human monocytic cell line thp-1. thirty minute exposures of thp-1 cells with lycd induced a 6 to 44-fold, dose-dependent increase in the ... | 2008 | 18473919 |
| mammalian mitochondria have the innate ability to import trnas by a mechanism distinct from protein import. | mitochondrial genomes generally encode a minimal set of trnas necessary for protein synthesis. however, a number of eukaryotes import trnas from the cytoplasm into their mitochondria. for instance, saccharomyces cerevisiae imports cytoplasmic trna(gln) into the mitochondrion without any added protein factors. here, we examine the existence of a similar active trna import system in mammalian mitochondria. we have used subcellular rna fractions from rat liver and human cells to perform rt-pcr with ... | 2008 | 18587046 |
| partial overlap of anti-mycobacterial, and anti-saccharomyces cerevisiae mannan antibodies in crohn's disease. | to test whether humoral immune reaction against mycobacteria may play a role in anti-saccharomyces cerevisiae antibodies (asca) generation in crohn's disease (cd) and/or whether it correlates with clinical subtypes. | 2008 | 18595132 |
| kibra modulates directional migration of podocytes. | asymmetric delivery and distribution of macromolecules are essential for cell polarity and for cellular functions such as differentiation, division, and signaling. injury of podocytes, which are polarized epithelial cells, changes the dynamics of the actin meshwork, resulting in foot process retraction and proteinuria. although the spatiotemporal control of specific protein-protein interactions is crucial for the establishment of cell polarity, the mechanisms controlling polarity-dependent diffe ... | 2008 | 18596123 |
| first total synthesis of clausine l and pityriazole, a metabolite of the human pathogenic yeast malassezia furfur. | the first total synthesis of the alkaloid pityriazole is described using three consecutive palladium-catalyzed coupling reactions. | 2008 | 18600266 |
| mafg controls the hypoxic response of cells by accumulating hif-1alpha in the nuclei. | we identified mafg as a protein that interacts with hif-1alpha, a key factor in hypoxic response, using the yeast two-hybrid system. interaction between mafg and hif-1alpha was confirmed by surface plasmon resonance and by translocation to the nucleolus with the nols signal. a knockdown of mafg reduced erythropoietin (epo) mrna levels as well as luciferase reporter activity with the hypoxia response element. the knockdown of mafg did not change total hif-1alpha protein, but reduced the accumulat ... | 2008 | 18538669 |
| integrated structural model and membrane targeting mechanism of the human escrt-ii complex. | escrt-ii plays a pivotal role in receptor downregulation and multivesicular body biogenesis and is conserved from yeast to humans. the crystal structures of two human escrt-ii complex structures have been determined at 2.6 and 2.9 a resolution, respectively. the complex has three lobes and contains one copy each of vps22 and vps36 and two copies of vps25. the structure reveals a dynamic helical domain to which both the vps22 and vps36 subunits contribute that connects the glue domain to the rest ... | 2008 | 18539118 |
| aspergillus nidulans arfb plays a role in endocytosis and polarized growth. | filamentous fungi undergo polarized growth throughout most of their life cycles. the spitzenkörper is an apical organelle composed primarily of vesicles that is unique to filamentous fungi and is likely to act as a vesicle supply center for tip growth. vesicle assembly and trafficking are therefore important for hyphal growth. adp ribosylation factors (arfs), a group of small gtpase proteins, play an important role in nucleating vesicle assembly. little is known about the role of arfs in filamen ... | 2008 | 18539885 |
| drosophila frataxin: an iron chaperone during cellular fe-s cluster bioassembly. | frataxin, a mitochondrial protein that is directly involved in regulating cellular iron homeostasis, has been suggested to serve as an iron chaperone during cellular fe-s cluster biosynthesis. in humans, decreased amounts or impaired function of frataxin causes the autosomal recessive neurodegenerative disorder friedreich's ataxia. cellular production of fe-s clusters is accomplished by the fe cofactor assembly platform enzymes isu (eukaryotes) and iscu (prokaryotes). in this report, we have cha ... | 2008 | 18540637 |
| parkin is ubiquitinated by nrdp1 and abrogates nrdp1-induced oxidative stress. | parkin plays an important role in the pathogenesis of parkinson's disease. we previously described that nrdp1, a ring-finger ubiquitin e3 ligase, interacted with parkin by the yeast two-hybrid assay and by co-immunoprecipitation. here we further demonstrated that overexpression of nrdp1 significantly reduced the endogenous parkin level in an nrdp1 dosage-dependent and proteasome-dependent manner. more importantly, nrdp1 ubiquitinated parkin and catalyzed the poly-ubiquitin chains on parkin in vi ... | 2008 | 18541373 |
| construction of cell surface-engineered yeasts displaying antigen to detect antibodies by immunofluorescence and yeast-elisa. | in order to detect monoclonal antibodies (mabs) from insufficient and unavailable human proteins, yeast cells were engineered to display human antigens on their surface and consequently endowed with the ability to specifically bind antibodies. thus, a fusion gene for the expression of the human proteasome subunit alpha 6 (hpsa6) and human profilin i (hproi) were assembled, respectively, with a his.tag marker at the c-terminal and displayed on yeast surface. with anti-his.tag mab as the primary a ... | 2008 | 18542951 |
| hur interacts with human immunodeficiency virus type 1 reverse transcriptase, and modulates reverse transcription in infected cells. | reverse transcription of the genetic material of human immunodeficiency virus type 1 (hiv-1) is a critical step in the replication cycle of this virus. this process, catalyzed by reverse transcriptase (rt), is well characterized at the biochemical level. however, in infected cells, reverse transcription occurs in a multiprotein complex - the reverse transcription complex (rtc) - consisting of viral genomic rna associated with viral proteins (including rt) and, presumably, as yet uncharacterized ... | 2008 | 18544151 |
| antibody complementarity-determining regions (cdrs) can display differential antimicrobial, antiviral and antitumor activities. | complementarity-determining regions (cdrs) are immunoglobulin (ig) hypervariable domains that determine specific antibody (ab) binding. we have shown that synthetic cdr-related peptides and many decapeptides spanning the variable region of a recombinant yeast killer toxin-like antiidiotypic ab are candidacidal in vitro. an alanine-substituted decapeptide from the variable region of this ab displayed increased cytotoxicity in vitro and/or therapeutic effects in vivo against various bacteria, fung ... | 2008 | 18545659 |
| tata binding protein associated factor 3 (taf3) interacts with p53 and inhibits its function. | the tumour suppressor protein p53 is a sequence specific dna-binding transcription regulator, which exerts its versatile roles in genome protection and apoptosis by affecting the expression of a large number of genes. in an attempt to obtain a better understanding of the mechanisms by which p53 transcription function is regulated, we studied p53 interactions. | 2008 | 18549481 |
| cleavage-mediated activation of chk1 during apoptosis. | the chk1 kinase is highly conserved from yeast to humans and is well known to function in the cell cycle checkpoint induced by genotoxic or replication stress. the activation of chk1 is achieved by atr-dependent phosphorylation with the aid of additional factors. robust genotoxic insults induce apoptosis instead of the cell cycle checkpoint, and some of the components in the atr-chk1 pathway are cleaved by active caspases, although it has been unclear whether the attenuation of the atr-chk1 path ... | 2008 | 18550533 |
| evidence for the formation of a covalent thiosulfinate intermediate with peroxiredoxin in the catalytic mechanism of sulfiredoxin. | the typical 2-cys peroxiredoxins are thiol-peroxidases involved in the physiology of hydrogen peroxide not only as a toxic but also as a signaling molecule. coordination of these functions depends on the sulfinylation of the catalytic cys, a modification reversed by atp-dependent sulfiredoxin, which specifically reduces the sulfinic acid group of overoxidized 2-cys peroxiredoxins into a sulfenic acid. sulfiredoxin was originally proposed to operate by covalent catalysis, with formation of a pero ... | 2008 | 18552404 |
| tata-binding protein recognition and bending of a consensus promoter are protein species dependent. | the structure and behavior of full-length human tbp binding the adenovirus major late promoter (admlp) have been characterized using biophysical methods. the human protein induces a 97 degrees bend in dna admlp. the high-resolution functional data provide a quantitative energetic and kinetic description of the partial reaction sequence as native human tbp binds rapidly to a consensus promoter with high affinity. the reaction proceeds with successive formation of three bound species, all having s ... | 2008 | 18553934 |
| plasmodium falciparum origin recognition complex subunit 5: functional characterization and role in dna replication foci formation. | the mechanism of dna replication initiation and progression is poorly understood in the parasites, including human malaria parasite plasmodium falciparum. using bioinformatics tools and yeast complementation assay, we identified a putative homologue of saccharomyces cerevisiaeorigin recognition complex subunit 5 in p. falciparum (pforc5). pforc5 forms distinct nuclear foci colocalized with the replication foci marker proliferating cell nuclear antigen (pfpcna) and co-immunoprecipitates with pcna ... | 2008 | 18554328 |
| presence of dominant negative mutation of tp53 is a risk of early recurrence in oral cancer. | genetic alteration of p53 is a significant determining factor in the carcinogenesis. the loss of function, mutant p53 can possess a dominant negative effect on wild-type p53 and may also exert gain-of-function activity. it is, however, not clear how p53 functional status due to various types of mutation results in outcome of patients with oral cancer. a total of 60 oral scc samples were subjected to yeast functional assay that screens human p53 function in yeast, and sequencing for determination ... | 2008 | 18555592 |
| ecsm2, an endothelial specific filamin a binding protein that mediates chemotaxis. | we aimed to characterize the expression and function of a novel transcript that bioinformatics analysis predicted to be endothelial specific, called endothelial-specific molecule-2 (ecsm2). | 2008 | 18556573 |
| regulation of rna polymerase iii transcription by maf1 protein. | maf1 was the first protein discovered to regulate polymerase iii rna in yeast and because it is evolutionarily conserved, a maf1 ortholog also serves to restrain transcription in mouse and human cells. understanding the mechanism of the regulation has been made possible by recent studies showing that maf1 is a nuclear/cytoplasmic protein whose subcellular distribution and hence negative regulation of pol iii transcription is mediated by the nutrient-sensing signaling pathways, tor and ras. under ... | 2008 | 18560610 |
| glutathione-dependent redox status of frataxin-deficient cells in a yeast model of friedreich's ataxia. | friedreich's ataxia is a neurodegenerative disease caused by reduced expression of the mitochondrial protein frataxin. the main phenotypic features of frataxin-deficient human and yeast cells include iron accumulation in mitochondria, iron-sulphur cluster defects and high sensitivity to oxidative stress. glutathione is a major protective agent against oxidative damage and glutathione-related systems participate in maintaining the cellular thiol/disulfide status and the reduced environment of the ... | 2008 | 18562474 |
| evolution of closely linked gene pairs in vertebrate genomes. | the orientation of closely linked genes in mammalian genomes is not random: there are more head-to-head (h2h) gene pairs than expected. to understand the origin of this enrichment in h2h gene pairs, we have analyzed the phylogenetic distribution of gene pairs separated by less than 600 bp of intergenic dna (gene duos). we show here that a lack of head-to-tail (h2t) gene duos is an even more distinctive characteristic of mammalian genomes, with the platypus genome as the only exception. in nonmam ... | 2008 | 18566020 |
| the mammalian target of rapamycin complex 2 controls folding and stability of akt and protein kinase c. | the target of rapamycin (tor), as part of the rapamycin-sensitive tor complex 1 (torc1), regulates various aspects of protein synthesis. whether tor functions in this process as part of torc2 remains to be elucidated. here, we demonstrate that mtor, sin1 and rictor, components of mammalian (m)torc2, are required for phosphorylation of akt and conventional protein kinase c (pkc) at the turn motif (tm) site. this torc2 function is growth factor independent and conserved from yeast to mammals. tm s ... | 2008 | 18566586 |
| repair of i-scei induced dsb at a specific site of chromosome in human cells: influence of low-dose, low-dose-rate gamma-rays. | we investigated the influence of low-dose, low-dose-rate gamma-ray irradiation on dna double strand break (dsb) repair in human lymphoblastoid tk6 cells. a single dsb was introduced at intron 4 of the tk+ allele (chromosome 17) by transfection with the i-scei expression vector pcbasce. we assessed for dsb repair due to non-homologous end-joining (nhej) by determining the generation of tk-deficient mutants in the tk6 derivative tsce5 (tk +/-) carrying an i-scei recognition site. we similarly esti ... | 2008 | 18568359 |
| prediction and analysis of nucleosome exclusion regions in the human genome. | nucleosomes are the basic structural units of eukaryotic chromatin, and they play a significant role in regulating gene expression. specific dna sequence patterns are known, from empirical and theoretical studies, to influence dna bending and flexibility, and have been shown to exclude nucleosomes. a whole genome localization of these patterns, and their analysis, can add important insights on the gene regulation mechanisms that depend upon the structure of chromatin in and around a gene. | 2008 | 18430246 |
| antihypertensive drug guanabenz is active in vivo against both yeast and mammalian prions. | prion-based diseases are incurable transmissible neurodegenerative disorders affecting animals and humans. | 2008 | 18431471 |
| conserved co-expression for candidate disease gene prioritization. | genes that are co-expressed tend to be involved in the same biological process. however, co-expression is not a very reliable predictor of functional links between genes. the evolutionary conservation of co-expression between species can be used to predict protein function more reliably than co-expression in a single species. here we examine whether co-expression across multiple species is also a better prioritizer of disease genes than is co-expression between human genes alone. | 2008 | 18433471 |
| global transcriptome and deletome profiles of yeast exposed to transition metals. | a variety of pathologies are associated with exposure to supraphysiological concentrations of essential metals and to non-essential metals and metalloids. the molecular mechanisms linking metal exposure to human pathologies have not been clearly defined. to address these gaps in our understanding of the molecular biology of transition metals, the genomic effects of exposure to group ib (copper, silver), iib (zinc, cadmium, mercury), via (chromium), and vb (arsenic) elements on the yeast saccharo ... | 2008 | 18437200 |
| histone deacetylase-related protein inhibits aes-mediated neuronal cell death by direct interaction. | histone deacetylase-related protein (hdrp), an alternatively spliced and truncated form of histone deacetylase-9 that lacks a c-terminal catalytic domain, protects neurons from death. in an effort to understand the mechanism by which hdrp mediates its neuroprotective effect, we screened for proteins in the brain that interact with hdrp by using a yeast two-hybrid assay. one of the hdrp-interacting proteins identified in this screen was amino enhancer of split (aes), a 197-amino acid protein belo ... | 2008 | 18438919 |
| toward a comprehensive temperature-sensitive mutant repository of the essential genes of saccharomyces cerevisiae. | the saccharomyces cerevisiae gene deletion project revealed that approximately 20% of yeast genes are required for viability. the analysis of essential genes traditionally relies on conditional mutants, typically temperature-sensitive (ts) alleles. we developed a systematic approach (termed "diploid shuffle") useful for generating a ts allele for each essential gene in s. cerevisiae and for improved genetic manipulation of mutant alleles and gene constructs in general. importantly, each ts allel ... | 2008 | 18439903 |
| lyric/aeg-1 overexpression modulates bccipalpha protein levels in prostate tumor cells. | lyric/aeg-1 is a unique protein that has been shown to promote tumor cell migration and invasion through activation of the transcription factor nf-kappab. we performed yeast two-hybrid screening to detect lyric/aeg-1 associated proteins, and identified bccip, a cdkn1a and brca2-associated protein involved in cell cycle regulation and dna repair. here, we demonstrate association between lyric/aeg-1 and bccip in mammalian cells, and define the region of interaction. co-expression of the two protei ... | 2008 | 18440304 |
| rapid unwinding of triplet repeat hairpins by srs2 helicase of saccharomyces cerevisiae. | expansions of trinucleotide repeats cause at least 15 heritable human diseases. single-stranded triplet repeat dna in vitro forms stable hairpins in a sequence-dependent manner that correlates with expansion risk in vivo. hairpins are therefore considered likely intermediates during the expansion process. unwinding of a hairpin by a dna helicase would help protect against expansions. yeast srs2, but not the recq homolog sgs1, blocks expansions in vivo in a manner largely dependent on its helicas ... | 2008 | 18440969 |
| the nucleocapsid protein of severe acute respiratory syndrome coronavirus inhibits cell cytokinesis and proliferation by interacting with translation elongation factor 1alpha. | severe acute respiratory syndrome coronavirus (sars-cov) is the etiological agent of sars, an emerging disease characterized by atypical pneumonia. using a yeast two-hybrid screen with the nucleocapsid (n) protein of sars-cov as a bait, the c terminus (amino acids 251 to 422) of the n protein was found to interact with human elongation factor 1-alpha (ef1alpha), an essential component of the translational machinery with an important role in cytokinesis, promoting the bundling of filamentous acti ... | 2008 | 18448518 |
| discovery, in vivo activity, and mechanism of action of a small-molecule p53 activator. | we have carried out a cell-based screen aimed at discovering small molecules that activate p53 and have the potential to decrease tumor growth. here, we describe one of our hit compounds, tenovin-1, along with a more water-soluble analog, tenovin-6. via a yeast genetic screen, biochemical assays, and target validation studies in mammalian cells, we show that tenovins act through inhibition of the protein-deacetylating activities of sirt1 and sirt2, two important members of the sirtuin family. te ... | 2008 | 18455128 |
| expression and maintenance of mitochondrial dna: new insights into human disease pathology. | mitochondria are central players in cellular energy metabolism and, consequently, defects in their function result in many characterized metabolic diseases. critical for their function is mitochondrial dna (mtdna), which encodes subunits of the oxidative phosphorylation complexes essential for cellular respiration and atp production. expression, replication, and maintenance of mtdna require factors encoded by nuclear genes. these include not only the primary machinery involved (eg, transcription ... | 2008 | 18458094 |
| bpoz-2 directly binds to eef1a1 to promote eef1a1 ubiquitylation and degradation and prevent translation. | bood poz containing gene type 2 (bpoz-2), which contains ankyrin repeats, nls, btb/poz domains and lxxll motifs, is an adaptor protein for the e3 ubiquitin ligase scaffold protein cul3. we isolated a cdna encoding eukaryotic elongation factor 1a1 (eef1a1) as a bpoz-2 binding protein by screening a human thymus cdna library using a yeast two-hybrid system. eef1a1 is essential for translation and is also involved in the 26s proteasome-dependent degradation of misfolded or unfolded proteins. the bi ... | 2008 | 18459963 |
| septin 7 interacts with centromere-associated protein e and is required for its kinetochore localization. | chromosome segregation in mitosis is orchestrated by dynamic interaction between spindle microtubules and the kinetochore. septin (sept) belongs to a conserved family of polymerizing gtpases localized to the metaphase spindle during mitosis. previous study showed that sept2 depletion results in chromosome mis-segregation correlated with a loss of centromere-associated protein e (cenp-e) from the kinetochores of congressing chromosomes (1). however, it has remained elusive as to whether cenp-e ph ... | 2008 | 18460473 |
| probiotics, prebiotics, and synbiotics. | according to the german definition, probiotics are defined viable microorganisms, sufficient amounts of which reach the intestine in an active state and thus exert positive health effects. numerous probiotic microorganisms (e.g. lactobacillus rhamnosus gg, l. reuteri, bifidobacteria and certain strains of l. casei or the l. acidophilus-group) are used in probiotic food, particularly fermented milk products, or have been investigated--as well as escherichia coli strain nissle 1917, certain entero ... | 2008 | 18461293 |
| mutation of trp29 of human equilibrative nucleoside transporter 1 alters affinity for coronary vasodilator drugs and nucleoside selectivity. | hent1 (human equilibrative nucleoside transporter 1) is inhibited by nanomolar concentrations of various structurally distinct coronary vasodilator drugs, including dipyridamole, dilazep, draflazine, soluflazine and nbmpr (nitrobenzylmercaptopurine ribonucleoside). when a library of randomly mutated hent1 cdnas was screened using a yeast-based functional complementation assay for resistance to dilazep, a clone containing the w29g mutation was identified. multiple sequence alignments revealed tha ... | 2008 | 18462193 |
| mutation at tyrosine in amlry (gilry like) motif of yeast erf1 on nonsense codons suppression and binding affinity to erf3. | termination translation in saccharomyces cerevisiae is controlled by two interacting polypeptide chain release factors, erf1 and erf3. two regions in human erf1, position at 281-305 and position at 411-415, were proposed to be involved on the interaction to erf3. in this study we have constructed and characterized yeast erf1 mutant at position 410 (correspond to 415 human erf1) from tyrosine to serine residue resulting erf1(y410s). the mutations did not affect the viability and temperature sensi ... | 2008 | 18463713 |
| huntingtin triplet-repeat locus is stable under long-term fen1 knockdown in human cells. | the influence of fen1 loss on trinucleotide-repeat expansion varies between species. in yeast, loss or haploinsufficiency of the fen1 homolog rad27 leads to triplet expansion. in mice, haploinsufficiency of fen1 leads to expansion of a huntingtin locus cag repeat. however, no expansion was seen of a (ctg)(n).(cag)(n) repeat in a myotonic dystrophy type 1 (dm1) knock-in model. in contrast, in drosophila, a sca7 cag90 repeat was completely stable in a series of strains with mutations of dna repair ... | 2008 | 18466979 |
| an in vivo map of the yeast protein interactome. | protein interactions regulate the systems-level behavior of cells; thus, deciphering the structure and dynamics of protein interaction networks in their cellular context is a central goal in biology. we have performed a genome-wide in vivo screen for protein-protein interactions in saccharomyces cerevisiae by means of a protein-fragment complementation assay (pca). we identified 2770 interactions among 1124 endogenously expressed proteins. comparison with previous studies confirmed known interac ... | 2008 | 18467557 |
| the vap protein family: from cellular functions to motor neuron disease. | the vamp-associated proteins (vaps) are highly conserved integral endoplasmic reticulum membrane proteins implicated in diverse cellular functions, including the regulation of lipid transport and homeostasis, membrane trafficking, neurotransmitter release, stabilization of presynaptic microtubules, and the unfolded protein response. recently, a single missense mutation within the human vap-b gene was identified in three forms of familial motor neuron disease. in this review, we integrate results ... | 2008 | 18468439 |
| c. elegans dss-1 is functionally conserved and required for oogenesis and larval growth. | dss1 (or rpn15) is a recently identified subunit of the 26s proteasome regulatory particle. in addition to its function in the protein degradation machinery, it has been linked to brca2 (breast cancer susceptibility gene 2 product) and homologous dna recombination, mrna export, and exocytosis. while the fungal orthologues of dss1 are not essential for viability, the significance of dss1 in metazoans has remained unknown due to a lack of knockout animal models. | 2008 | 18471277 |
| comparative study on the antioxidant and biological activities of carvacrol, thymol, and eugenol derivatives. | four derivatives of thymol, carvacrol, and eugenol were synthesized: 4-(hydroxymethyl)-5-isopropyl-2-methylphenol, 4,4'-methylenebis(5-isopropyl-2-methyl)phenol, 4-allyl-6-(hydroxymethyl)-2-methoxyphenol, and 4-(hydroxymethyl)-2-isopropyl-5-methylphenol. the obtained derivatives showed remarkably better antioxidative properties according to 1,1-diphenyl-2-picrylhydrazyl assay (50% inhibitory concentrations = 4-156 microg/ml) and rancimat assay (protection factors = 1.55-5.84) when compared with ... | 2008 | 18473475 |
| arabidopsis mrna polyadenylation machinery: comprehensive analysis of protein-protein interactions and gene expression profiling. | the polyadenylation of mrna is one of the critical processing steps during expression of almost all eukaryotic genes. it is tightly integrated with transcription, particularly its termination, as well as other rna processing events, i.e. capping and splicing. the poly(a) tail protects the mrna from unregulated degradation, and it is required for nuclear export and translation initiation. in recent years, it has been demonstrated that the polyadenylation process is also involved in the regulation ... | 2008 | 18479511 |
| lkb1 and lung cancer: more than the usual suspects. | often, the problem in cancer research is figuring out how a gene or pathway works in regulating cellular transformation. the question of what ras activates or pten inhibits have been classic dilemmas of modern cancer biology. in these cases, biochemical and genetic studies have provided us with a fairly clear picture of the cancer relevant functions of these genes. for lkb1, a more recently identified human tumor suppressor gene, however, the problem is different. this serine-threonine kinase th ... | 2008 | 18483235 |
| the small ubiquitin-like modifier (sumo) and sumo-conjugating system of chlamydomonas reinhardtii. | the availability of the complete dna sequence of the chlamydomonas reinhardtii genome and advanced computational biology tools has allowed elucidation and study of the small ubiquitin-like modifier (sumo) system in this unicellular photosynthetic alga and model eukaryotic cell system. sumo is a member of a ubiquitin-like protein superfamily that is covalently attached to target proteins as a post-translational modification to alter the localization, stability, and/or function of the target prote ... | 2008 | 18493050 |
| triple-helix structure in telomerase rna contributes to catalysis. | telomerase is responsible for replication of the ends of linear chromosomes in most eukaryotes. its intrinsic rna subunit provides the template for synthesis of telomeric dna by the reverse-transcriptase (tert) subunit and tethers other proteins into the ribonucleoprotein (rnp) complex. we report that a phylogenetically conserved triple helix within a pseudoknot structure of this rna contributes to telomerase activity but not by binding the tert protein. instead, 2'-oh groups protruding from the ... | 2008 | 18500353 |
| expression and purification of active recombinant human bikunin in pichia pastoris. | bikunin is a proteoglycan exhibiting broad-spectrum inhibitory activity against serine proteases and could potentially suppress tumor cell invasion and metastasis. here, we have successfully expressed recombinant human bikunin (rh-bikunin) in the methylotrophic yeast pichia pastoris and established the purification procedure. the cdna encoding human bikunin was cloned by pcr and inserted into the expression vector ppiczalphac. after expressed in shake flask, rh-bikunin was produced in an 80-l fe ... | 2008 | 18501630 |
| a family of cathepsin b cysteine proteases expressed in the gut of the human hookworm, necator americanus. | mrnas encoding cathepsin b-like cysteine proteases (catbs) are abundantly expressed in the genomes of blood-feeding nematodes. recombinant catbs have been partially efficacious in vaccine trials in animal models of hookworm infection, supporting further investigation of these enzymes as new control tools. we recently described a family of four distinct catbs (na-cp-2, -3, -4, -5) from the human hookworm, necator americanus. here we show that these n. americanus catbs form a robust clade with oth ... | 2008 | 18501979 |
| mutant forms of the extracellular domain of the human acetylcholine receptor gamma-subunit with improved solubility and enhanced antigenicity. the importance of the cys-loop. | the muscle nicotinic acetylcholine receptor (achr) is the prototype of the ligand-gated ion channels (or cys-loop receptors), formed by 5 homologous subunits (alpha2betagammadelta or alpha2betagammaepsilon), and is the major autoantigen in the autoimmune disease, myasthenia gravis. previously, we expressed the wild-type extracellular domain (ecd) of the gamma-subunit (gammaecd) of the achr in yeast pichia pastoris at 0.3-0.8 mg/l, in soluble but microaggregate form, to use as starting material f ... | 2008 | 18502212 |
| virion-wide protein interactions of kaposi's sarcoma-associated herpesvirus. | herpesvirus virions are highly organized structures built through specific protein-protein interactions. thus, revelation of the protein interactions among virion proteins will shed light on the processes and the mechanisms of virion formation. recently, we identified 24 virion proteins of kaposi's sarcoma-associated herpesvirus (kshv), using a proteomic approach (f. x. zhu et al., j. virol. 79:800-811, 2005). in the current study, a comprehensive analysis of protein-protein interaction between ... | 2008 | 18321973 |
| interaction between fortilin and transforming growth factor-beta stimulated clone-22 (tsc-22) prevents apoptosis via the destabilization of tsc-22. | yeast two-hybrid screening was conducted using a human ovary cdna library to search for a novel binding protein using transforming growth factor-beta stimulated clone-22 (tsc-22). the selected protein was fortilin, which has been characterized as a nuclear anti-apoptotic protein. overexpression of fortilin in ovarian carcinoma cells reversed tsc-22-mediated apoptosis, and the inhibition of fortilin expression via small interfering rna (sirna) resulted in an increase in the apoptosis of ovarian c ... | 2008 | 18325344 |
| generation of novel monoclonal antibodies and their application for detecting ard1 expression in colorectal cancer. | arrest defective 1 (ard1) is an acetyltransferase involved in cell cycle control in yeast. ard1 interacts with human n-acetyltransferase (nath) to form a functional n-terminal acetyltransferase complex. recently it had been linked with proliferation and apoptosis in mammalian cells, but its function in cancer development remains unclear. to evaluate significance of ard1 expression in human colorectal cancer, we generated a panel of monoclonal antibodies (mabs) with high specificity and sensitivi ... | 2008 | 18325661 |
| is there a serious risk of resistance development to azoles among fungi due to the widespread use and long-term application of azole antifungals in medicine? | it is well known that development of antibiotic resistance in bacteria is not a matter of if but of when. recently, azoles have been recommended for long-term prophylaxis of invasive fungal infections; hence, it could be argued that fungi also will become resistant to these agents. however, fungi are different from bacteria in several critical points. bacteria display several resistance mechanisms: alteration of the target, limited access to the target and modification/inactivation of the antiba ... | 2008 | 18325827 |
| novel oxidative stress-responsive gene ers25 functions as a regulator of the heat-shock and cell death response. | members of the yeast p24 family, including emp24p and erv25p, exist as heteromeric complexes that have been proposed to cycle between the endoplasmic reticulum (er) and golgi compartments. the specific functions and sites of action of p24 proteins are still unknown. here we identified a human homolog of the yeast p24 family of proteins, named ers25 (endoplasmic reticulum stress-response protein 25), and investigated its role in stress response. ers25 is predicted to have an er localization signa ... | 2008 | 18326488 |
| identification of tom5 and tom6 in the preprotein translocase complex of human mitochondrial outer membrane. | the fungal preprotein translocase of the mitochondrial outer membrane (tom complex) comprises import receptors tom70, tom20, and tom22, import channel tom40, and small tom proteins tom5, tom6, and tom7, which regulate tom complex assembly. these components are conserved in mammals; unlike the other components, however, tom5 and tom6 remain unidentified in mammals. we immuno-isolated the tom complex from hela cells expressing htom22-flag and identified the human counterparts of tom5 and tom6, tog ... | 2008 | 18331822 |
| development of a mlst-biased snp microarray for candida albicans. | we have developed a single nucleotide polymorphism (snp) detection microarray for the human pathogenic yeast, candida albicans, consisting of synthetic oligonucleotides bound to microscope slides. the array consists of multiple replicates of 79 snps, derived from 19 discrete loci located on all eight chromosomes. these loci include seven genes consisting of 57 snps that comprise a multi-locus sequence typing (mlst) consensus scheme for the species. the remaining 22 snps are from 12 additional lo ... | 2008 | 18334305 |
| crystal structures of fission yeast histone chaperone asf1 complexed with the hip1 b-domain or the cac2 c terminus. | the assembly of core histones onto eukaryotic dna is modulated by several histone chaperone complexes, including asf1, caf-1, and hira. asf1 is a unique histone chaperone that participates in both the replication-dependent and replication-independent pathways. here we report the crystal structures of the apo-form of fission yeast asf1/cia1 (spasf1n; residues 1-161) as well as its complexes with the b-domain of the fission yeast hira orthologue hip1 (hip1b) and the c-terminal region of the cac2 s ... | 2008 | 18334479 |