Publications

comprehensive in silico allergenicity assessment of novel protein engineered chimeric cry proteins for safe deployment in crops.	development of chimeric cry toxins by protein engineering of known and validated proteins is imperative for enhancing the efficacy and broadening the insecticidal spectrum of these genes. expression of novel cry proteins in food crops has however created apprehensions with respect to the safety aspects. to clarify this, premarket evaluation consisting of an array of analyses to evaluate the unintended effects is a prerequisite to provide safety assurance to the consumers. additionally, series of ...	2017	28768539
biodegradation of anthracene by a newly isolated bacterial strain, bacillus thuringiensis at.ism.1 isolated from a fly ash deposition site.	current study is aimed to evaluate the mechanism of anthracene degradation by a bacterial strain isolated from fly ash deposition site near jamadoba coal preparation plant, jharkhand, india. the bushnell-haas media cultured (containing anthracene as sole carbon source) bacterial isolate was identified by 16s rrna gene sequence coding as the bacillus thuringiensis strain, which showed the efficiency to degrade anthracene. the degradation efficiency of the strain has been estimated around 91% (for ...	2017	28763108
is there a transgenerational inheritance of host resistance against pathogens? lessons from the galleria mellonella-bacillus thuringiensis interaction model.		2017	28758839
mosquitocidal potential of silver nanoparticles synthesized using local isolates of bacillus thuringiensis subsp. israelensis and their synergistic effect with a commercial strain of b. thuringiensis subsp. israelensis.	control of larval stages of aedes aegypti is considered an effective approach for preventing outbreaks of dengue fever. in this work, silver nanoparticles (ag nps) were synthesized using the supernatant and insecticidal proteins from local isolates of bacillus thuringiensis subsp. israelensis (bti). mosquitocidal activity assays against a. aegypti larvae revealed that the highest toxicity was obtained from the ag nps synthesized using supernatant of bti k55 and the inclusion proteins of bti k46 ...	2017	28754251
knockout of a p-glycoprotein gene increases susceptibility to abamectin and emamectin benzoate in spodoptera exigua.	p-glycoprotein [p-gp or the atp-binding cassette transporter b1 (abcb1)] is an important participant in multidrug resistance of cancer cells, yet the precise function of this arthropod transporter is unknown. the aim of this study was to determine the importance of p-gp for susceptibility to insecticides in the beet armyworm (spodoptera exigua) using clustered regularly interspaced short palindromic repeats/crispr-associated 9 (crispr/cas9) gene-editing technology. we cloned an open reading fram ...	2017	28753233
improving insect control protein activity for gm crops: a case study demonstrating that increased target insect potency can be achieved without impacting mammalian safety.	many insect-protected crops express insecticidal crystal (cry) proteins derived from the soil bacterium bacillus thuringiensis (bt), including both naturally-occurring cry proteins and chimeric cry proteins created through biotechnology. the cry51aa2 protein is a naturally-occurring cry protein that was modified to increase its potency and expand its insect activity spectrum through amino acid sequence changes. the improved cry51aa2 variant, cry51aa2.834_16, and other developmental variants belo ...	2017	28751263
chrysodeixis chalcites nucleopolyhedrovirus (chchnpv): natural occurrence and efficacy as a biological insecticide on young banana plants in greenhouse and open-field conditions on the canary islands.	chrysodeixis chalcites, an important pest of banana crops on the canary islands, is usually controlled by chemical insecticides. the present study aimed to evaluate the efficacy of the most prevalent isolate of the chrysodeixis chalcites nucleopolyhedrovirus (chchnpv, baculoviridae) as a biological insecticide. overall the prevalence of chchnpv infection in c. chalcites populations was 2.3% (103 infected larvae out of 4,438 sampled), but varied from 0-4.8% on tenerife and was usually low (0-2%) ...	2017	28750003
dissecting the dynamics of trans-generational immune priming.	the transfer of immunity from mother to offspring is a central way to endow the offspring with increased protection against pathogens. this phenomenon is not only found within the vertebrate domain: in some circumstances, invertebrate mothers can also give their offspring an immune kick-start, which is termed trans-generational immune priming (tgip). tgip has been uncovered for a number of invertebrate species, but it is not ubiquitously evident. the reasons for which are not known. in this issu ...	2017	28749614
separation, quantification and mosquito larvicidal activity of insecticidal crystal proteins of bacillus thuringiensis var. israelensis (vcrc b17; mtcc 5596).		2017	28748842
dissecting the contributions of time and microbe density to variation in immune gene expression.	widespread differential expression of immunological genes is a hallmark of the response to infection in almost all surveyed taxa. however, several challenges remain in the attempt to connect differences in gene expression with functional outcomes like parasite killing and host survival. for example, temporal gene expression patterns are not always monotonic (unidirectional slope), yielding results that qualitatively depend on the time point selected for analysis. they may also be correlated to m ...	2017	28747473
action of natural phytosanitary products on bacillus thuringiensis subsp. kurstaki s-1905.	the objective of this study was to evaluate the effects of natural phytosanitary products (nps) on spores and crystals of bacillus thuringiensis subsp. kurstaki s-1905 (btk s-1905). for the spore assay, nps and bacteria were applied in combination and individually. for the combined application, btk s-1905 + np mixtures were inoculated on nutrient agar (na), and for the separate applications, the nps were spread on na plates, which were later inoculated with the pathogen. the number of colony-for ...	2017	28743317
comparative molecular analyses of invasive fall armyworm in togo reveal strong similarities to populations from the eastern united states and the greater antilles.	the fall armyworm (spodoptera frugiperda, j.e. smith) is a noctuid moth that is a major and ubiquitous agricultural pest in the western hemisphere. infestations have recently been identified in several locations in africa, indicating its establishment in the eastern hemisphere where it poses an immediate and significant economic threat. genetic methods were used to characterize noctuid specimens infesting multiple cornfields in the african nation of togo that were tentatively identified as fall ...	2017	28738081
probiotic enterococcus mundtii isolate protects the model insect tribolium castaneum against bacillus thuringiensis.	enterococcus mundtii strains isolated from the larval feces of the mediterranean flour moth ephestia kuehniella show antimicrobial activity against a broad spectrum of gram-positive and gram-negative bacteria. the in vitro probiotic characterization of one isolate revealed a high auto-aggregation score, a hydrophilic cell surface, tolerance for low ph, no hemolytic activity, and susceptibility to all tested antibiotics. we used the red flour beetle tribolium castaneum, an established model organ ...	2017	28736550
foxa transcriptional factor modulates insect susceptibility to bacillus thuringiensis cry1ac toxin by regulating the expression of toxin-receptor abcc2 and abcc3 genes.	cry toxins produced by bacillus thuringiensis (bt) are insecticidal proteins widely used in insect control. recently, it was shown that atp-binding cassette transporter proteins (abc) such as abcc2, abcc3, abcg1 and abca2 are implicated in the insecticidal action of cry toxins as putative receptors. however, the transcriptional regulators involved in the expression of abc transporter genes remain unknown. sequence analysis of promoter regions of abcc2 gene from helicoverpa armigera and abcc3 gen ...	2017	28736301
knockdown of two cadherin genes confers resistance to cry2a and cry1c in chilo suppressalis.	bacillus thuringiensis (bt) cry toxins play an important role in the management of insect pests. resistance to bt toxins has been reported in many pest insects but the mechanism responsible for this resistance in rice crop pests remains largely unknown. cadherin is one of several bt toxin receptors. at present, only one cadherin gene, cscad1, has been documented in the striped rice stem borer, chilo suppressalis. we amplified a nearly full-length transcript of another c. suppressalis cadherin ge ...	2017	28729614
bacillus thuringiensis: an annotated selection of world wide web sites relevant to the topics in environmental microbiology.		2017	28727324