Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| in vitro antioxidant, antimicrobial and anti-proliferative activities of purple potato extracts (solanum tuberosum cv vitelotte noire) following simulated gastro-intestinal digestion. | analyses of antioxidant and in vitro antimicrobial and anti-proliferative activities of anthocyanin-rich extracts from purple potatoes, solanum tuberosum l. cv vitelotte noire (solanaceae), were performed by simulating both a domestic cooking process and human digestion. extracts of crude and cooked purple potato did not exhibit antimicrobial activity against the tester strains: bacillus cereus, escherichia coli and pseudomonas aeruginosa. the behaviour changed after the simulated gastrointestin ... | 2015 | 25420792 |
| prevalence, genetic diversity, and antibiotic resistance of bacillus cereus isolated from korean fermented soybean products. | bacillus cereus contamination is a major food safety problem for korean fermented soybean products, but few studies have assessed its potential to cause foodborne illness. the objectives of this study were to investigate the prevalence and characteristics of b. cereus isolated from korean fermented soybean products. b. cereus was detected in 110 of 162 (67.9%) samples. the highest b. cereus frequency was observed in deonjang (68 of 93 samples, 73.1%) and cheonggukjang (18 of 25, 72.0%); however, ... | 2015 | 25472031 |
| the analysis of distribution of multidrug resistant pseudomonas and bacillus species from burn patients and burn ward environment. | infections caused by multidrug resistant bacteria act as a risk factor for mortality in burns patients. so keeping in view the crucial importance of reliable therapeutic decisions of multidrug resistance bacteria and role of hospital environment in bacteria colonization, our study is based on the evaluation of distribution of pseudomonas sp. and bacillus sp. in burn patients and burn ward environment. | 2015 | 25468476 |
| effect of packaging technology on microbiological and sensory quality of a cooked blood sausage, morcela de arroz, from monchique region of portugal. | morcela de arroz (ma), a popular portuguese blood sausage, with high ph and water activity (aw), is traditionally commercialized without preservatives and unpacked. this study evaluated the best packaging solution to extend ma shelf life stored at 4±1°c for 44days: without packaging (wp), vacuum (vp) and modified atmosphere packaging (map) (80% co2; 20% n2). mesophilic (mtvc), psychrotrophic (ptvc), lactic acid bacteria (lab), pseudomonads, molds and yeasts, enterobacteriaceae, listeria monocyto ... | 2015 | 25462380 |
| integrative approach to produce hydrogen and polyhydroxybutyrate from biowaste using defined bacterial cultures. | biological production of hydrogen (h2) and polyhydroxybutyrate (phb) from pea-shell slurry (pss) was investigated using defined mixed culture (mmc4, composed of enterobacter, proteus, bacillus spp.). under batch culture, 19.0lh2/kg of pss (total solid, ts, 2%w/v) was evolved. using effluent from the h2 producing stage, bacillus cereus egu43 could produce 12.4% (w/w) phb. dilutions of pss hydrolysate containing glucose (0.5%, w/v) resulted in 45-75lh2/kg ts fed and 19.1% (w/w) of phb content. und ... | 2015 | 25460994 |
| aerobic biodegradation of odorous dimethyl disulfide in aqueous medium by isolated bacillus cereus gigan2 and identification of transformation intermediates. | a novel, flagellated, rod-shape, gram-positive facultative aerobe, was isolated and identified as bacillus cereus gigan2. it can effectively remove model odorous organics dimethyl disulfide (dmds) in aqueous solution under aerobic conditions. initial concentration, ph value and temperature played important role in dmds biodegradation, and up to 100% of 10mgl(-1) of dmds could be removed within 96h under the optimum conditions (30°c, ph 7.0 and 200rpm) with a maximum biodegradation rate constant ... | 2015 | 25459868 |
| antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of calotropis gigantea l. | the present study aimed to synthesis silver nanoparticles (agnps) in a greener route using aqueous latex extract of calotropis gigantea l. toward biomedical applications. initially, synthesis of agnps was confirmed through uv-vis spectroscopy which shows the surface plasmonic resonance peak (spr) at 420 nm. fourier transform infrared spectroscopy (ftir) analysis provides clear evidence that protein fractions present in the latex extract act as reducing and stabilizing bio agents. energy dispersi ... | 2015 | 25459618 |
| an emetic bacillus cereus outbreak in a kindergarten: detection and quantification of critical levels of cereulide toxin. | a bacillus cereus-related emetic outbreak was reported in a belgian kindergarten. high levels of emetic b. cereus (>1.5e+07 colony-forming units/g) were detected in the food leftovers, and the presence of an emetic strain was confirmed in feces. emetic toxin levels ranging up to 4.2 μg/g were also quantified in the leftovers by liquid chromatography coupled to tandem mass spectrometry (lc-ms(2)) analysis. those levels, although moderate in comparison with earlier published intoxications, provoke ... | 2015 | 25457101 |
| brex is a novel phage resistance system widespread in microbial genomes. | the perpetual arms race between bacteria and phage has resulted in the evolution of efficient resistance systems that protect bacteria from phage infection. such systems, which include the crispr-cas and restriction-modification systems, have proven to be invaluable in the biotechnology and dairy industries. here, we report on a six-gene cassette in bacillus cereus which, when integrated into the bacillus subtilis genome, confers resistance to a broad range of phages, including both virulent and ... | 2015 | 25452498 |
| association of a zn(2+) containing metallo β-lactamase with the anticancer antibiotic mithramycin. | pathogenic bacteria that are resistant to β-lactam antibiotics mostly utilize serine β-lactamases to degrade the antibiotics. current studies have shown that different subclasses of metallo β-lactamases (e[mbl]) are involved in the defense mechanism of drug resistant bacteria. here we report that the zn(2+) containing subclass b1 e[mbl] from bacillus cereus binds to a naturally occurring anti-cancer drug mithramycin (mtr). spectroscopic (cd and fluorescence) and isothermal titration calorimetry ... | 2015 | 25450021 |
| establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in saccharomyces cerevisiae via β-alanine. | microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global co2 emissions. 3-hydroxypropionic acid (3hp) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. with the objective of developing saccharomyces cerevisiae as an efficient cell factory for high-level production of 3hp, we identified the β-alanine biosynthetic route as the most economically attractive according to t ... | 2015 | 25447643 |
| single cell profiling of surface carbohydrates on bacillus cereus. | cell surface carbohydrates are important to various bacterial activities and functions. it is well known that different types of bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved. here, using atomic force microscopy (afm)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of n-acetylglucosamine and m ... | 2015 | 25505137 |
| a common active site of polyhydroxyalkanoate synthase from bacillus cereus yb-4 is involved in polymerization and alcoholysis reactions. | polyhydroxyalkanoate (pha) synthase from bacillus cereus yb-4 (pharcyb4) catalyzes not only pha polymerization but also alcoholytic cleavage of pha chains. the alcoholysis activity of pharcyb4 is expressed when a hydroxyacyl-coa monomer is absent but an alcohol compound is present. in this study, we performed alanine mutagenesis of the putative catalytic triad (cys(151), asp(306), and his(335)) in the phacyb4 subunit to identify the active site residues for polymerization and alcoholysis activit ... | 2015 | 25503319 |
| microbiome of the placenta in pre-eclampsia supports the role of bacteria in the multifactorial cause of pre-eclampsia. | this study was aimed at detecting, identifying, quantifying and comparing the bacteria present in the placental tissues of women with pre-eclampsia with that of normotensive pregnant women. | 2015 | 25492799 |
| bacillus cereus in personal care products: risk to consumers. | bacillus cereus is ubiquitous in nature and thus occurs naturally in a wide range of raw materials and foodstuffs. b. cereus spores are resistant to desiccation and heat and able to survive dry storage and cooking. vegetative cells produce several toxins which on ingestion in sufficient numbers can cause vomiting and/or diarrhoea depending on the toxins produced. gastrointestinal disease is commonly associated with reheated or inadequately cooked foods. in addition to being a rare cause of sever ... | 2015 | 25482451 |
| reducing activity, glucose metabolism and acid tolerance response of bacillus cereus grown at various ph and oxydo-reduction potential levels. | bacillus cereus is a major foodborne bacterial pathogen able to survive a large number of physical-chemical stresses. b. cereus encounters different ph and redox potential (eh7) levels during its passage through the gastrointestinal tract. analysis of the combined influence of ph and redox stresses on b. cereus f4430/73 physiology found that b. cereus f4430/73 growth at ph 7.0 at 37 °c had strong reducing capacities, with a total change of 315 mv from an initial redox value of +214 ± 17 mv. the ... | 2015 | 25475301 |
| genetic discrimination of foodborne pathogenic and spoilage bacillus spp. based on three housekeeping genes. | bacillus genus includes foodborne pathogenic and spoilage-associated species, such as bacillus cereus, bacillus licheniformis, bacillus subtilis and bacillus pumilus. bacillus is also a heterogeneous genus that includes closely related species that are difficult to discriminate among, especially when well-conserved genes such as 16s rrna and 23s rrna are considered. the main goal of the present work was to study the usefulness of three housekeeping genes, the tu elongation factor (tuf), the dna ... | 2015 | 25475298 |
| a case of intoxication due to a highly cytotoxic bacillus cereus strain isolated from cooked chicken. | outbreaks of bacillus cereus infection/intoxication are not commonly reported because symptoms are often mild, and the disease is self-limiting. however, hypervirulent strains increase health risks. we report a case, which occurred in argentina, of severe food poisoning illness on a healthy adult woman associated to b. cereus strain mvl2011. the studied strain was highly cytotoxic, showed high ability to detach caco-2 cells and was positive for the hbla, hblb, and hblc genes of the hbl complex, ... | 2015 | 25475284 |
| novel polycarboxylate porphyrins: synthesis, characterization, photophysical properties and preliminary antimicrobial study against gram-positive bacteria. | we describe the synthesis, characterization and photophysical properties of two new polycarboxylic photosensitizers. owing to their structural design, these two compounds show water solubilities larger than natural carboxylic photosensitizers (e.g., protoporphyrin ix, hematoporphyrin, etc.) and also good singlet oxygen quantum yields. these compounds were tested as photo-antimicrobial agents against staphylococcus aureus and bacillus cereus strains. results reveal that their photocytotoxicities ... | 2015 | 25475206 |
| characterization of a novel thermostable n-acylhomoserine lactonase from the thermophilic bacterium thermaerobacter marianensis. | thermaerobacter marianensis is an extremely thermophilic bacterium, which was isolated from the mariana trench, with an optimal growth temperature of approximately 75 °c. n-acylhomoserine lactone (ahl) is a quorum-sensing signal molecule used by many gram-negative bacteria. here, we report the identification of an ahl-degrading gene homolog (designated aiit) in the genome of t. marianensis jcm 10246. aiit has 59.7%, 21.2%, and 11.2% identity to ahls from solibacillus silvestris, aiia from bacill ... | 2015 | 25529553 |
| characteristics and phylogeny of bacillus cereus strains isolated from maari, a traditional west african food condiment. | maari is a spontaneously fermented food condiment made from baobab tree seeds in west african countries. this type of product is considered to be safe, being consumed by millions of people on a daily basis. however, due to the spontaneous nature of the fermentation the human pathogen bacillus cereus occasionally occurs in maari. this study characterizes succession patterns and pathogenic potential of b. cereus isolated from the raw materials (ash, water from a drilled well (dw) and potash), seed ... | 2015 | 25528535 |
| spirotetronate antibiotics with anti-clostridium activity from actinomadura sp. 2eps. | the rare actinomycetes strain 2eps was isolated from soil and analysis of cultural, morphological characteristics, diaminopimelic acid content of its cell wall, and 16s rrna gene sequence indicates that 2eps belongs to genus actinomadura. in addition, neighbor-joining phylogenetic tree also confirmed the relationships of this strain to other members of actinomadura. a butanol extract with antibacterial activity was purified by reversed-phase chromatography to obtain three bioactive compounds, de ... | 2015 | 25543910 |
| biosynthesis of hematite nanoparticles and its cytotoxic effect on hepg2 cancer cells. | iron oxide nanoparticles were gaining significant importance in a variety of applications due to its paramagnetic properties and biocompatibility. various chemical methods were employed for hematite nanoparticle synthesis which require special equipment or a complex production process. in this study, protein capped crystalline hexagonal hematite (α-fe2o3) nanoparticles were synthesized by green approach using culture supernatant of a newly isolated bacterium, bacillus cereus svk1 at ambient cond ... | 2015 | 25542166 |
| toxigenic potential and heat survival of spore-forming bacteria isolated from bread and ingredients. | fifty-four spore-forming bacterial strains isolated from bread ingredients and bread, mainly belonging to the genus bacillus (including bacillus cereus), together with 11 reference strains were investigated to evaluate their cytotoxic potential and heat survival in order to ascertain if they could represent a risk for consumer health. therefore, we performed a screening test of cytotoxic activity on ht-29 cells using bacterial culture filtrates after growing bacterial cells in brain heart infusi ... | 2015 | 25555227 |
| enhancement of ε-poly-l-lysine (ε-pl) production by a novel producer bacillus cereus using metabolic precursors and glucose feeding. | epsilon poly-l-lysine (ε-pl) is a homo-biopolymer with approximately 25-30 l-lysine residues. it is a promising natural biopolymer widely used in food and pharmaceutical industry. the present work reports enhanced production of ε-pl with a novel producer bacillus cereus using amino acids and tca cycle intermediates in the fermentation medium. among the various amino acids and tca cycle intermediates tested 2 mm l-aspartic acid and 5 mm citric acid gave ε-pl yield of 145.5 and 230 mg/l, respectiv ... | 2015 | 28324535 |
| antimicrobial activities of three medicinal plants and investigation of flavonoids of tripleurospermum disciforme. | rosa damascena, tripleurospermum disciforme and securigera securidaca were used as disinfectant agents and for treatment of some disease in folk medicine of iran. the antimicrobial effects of different fractions of seeds extract of s. securidaca, petals extract of r. damascena and aerial parts extract of t. disciforme were examined against some gram positive, gram negative and fungi by cup plate diffusion method. the petroleum ether and chloroform fractions of s. securidaca showed antibacterial ... | 2015 | 25561928 |
| improved efficiency and stability of secnidazole - an ideal delivery system. | secnidazole (α,2-dimethyl-5-nitro-1h-imidazole-1-ethanol) is a highly effective drug against a variety of g(+)/g(-) bacteria but with significant side effects because it is being used in very high concentration. in this study, gold nanoparticles (gnps) were selected as a vehicle to deliver secnidazole drug at the specific site with more accuracy which made the drug highly effective at substantially low concentrations. the as-synthesized gnps were capped with human serum albumin (hsa) and subsequ ... | 2015 | 25561882 |
| carvacrol suppresses high pressure high temperature inactivation of bacillus cereus spores. | the inactivation of bacterial spores generally proceeds faster and at lower temperatures when heat treatments are conducted under high pressure, and high pressure high temperature (hpht) processing is, therefore, receiving an increased interest from food processors. however, the mechanisms of spore inactivation by hpht treatment are poorly understood, particularly at moderately elevated temperature. in the current work, we studied inactivation of the spores of bacillus cereus f4430/73 by hpht tr ... | 2015 | 25560915 |
| characteristics of enterotoxin distribution, hemolysis, lecithinase, and starch hydrolysis of bacillus cereus isolated from infant formulas and ready-to-eat foods. | bacillus cereus is a ubiquitous environmental microbe implicated as a main cause of food poisoning with various symptoms, depending on the strain type and the isolation source. in this study, the potential virulence factors and biochemical properties of b. cereus isolated from infant formulas and ready-to-eat (rte) foods were analyzed and compared. a total of 347 b. cereus strains were isolated and identified from 687 infant food formulas and rte food samples. all the isolates had one or more en ... | 2015 | 25597976 |
| bacteriophage pbc1 and its endolysin as an antimicrobial agent against bacillus cereus. | bacillus cereus is an opportunistic human pathogen responsible for food poisoning and other, nongastrointestinal infections. due to the emergence of multidrug-resistant b. cereus strains, the demand for alternative therapeutic options is increasing. to address these problems, we isolated and characterized a siphoviridae virulent phage, pbc1, and its lytic enzymes. pbc1 showed a very narrow host range, infecting only 1 of 22 b. cereus strains. phylogenetic analysis based on the major capsid prote ... | 2015 | 25595773 |
| finished genome sequence of bacillus cereus strain 03bb87, a clinical isolate with b. anthracis virulence genes. | bacillus cereus strain 03bb87, a blood culture isolate, originated in a 56-year-old male muller operator with a fatal case of pneumonia in 2003. here we present the finished genome sequence of that pathogen, including a 5.46-mb chromosome and two plasmids (209 and 52 kb, respectively). | 2015 | 25593267 |
| antimicrobial potential of cauliflower, broccoli, and okara byproducts against foodborne bacteria. | the antimicrobial potential of cauliflower, broccoli, and okara byproducts was assessed against gram-positive and gram-negative bacteria. salmonella enterica serovar typhimurium, escherichia coli o157:h7, bacillus cereus, and listeria monocytogenes serovar 4b growth behavior was assessed under exposure to 5% vegetable byproducts added to the reference medium, buffered peptone water (0.1% [wt/vol]), at 37°c. although the byproducts were not effective against l. monocytogenes, they were bactericid ... | 2015 | 25587927 |
| slow leakage of ca-dipicolinic acid from individual bacillus spores during initiation of spore germination. | when exposed to nutrient or nonnutrient germinants, individual bacillus spores can return to life through germination followed by outgrowth. laser tweezers, raman spectroscopy, and either differential interference contrast or phase-contrast microscopy were used to analyze the slow dipicolinic acid (dpa) leakage (normally ∼20% of spore dpa) from individual spores that takes place prior to the lag time, tlag, when spores begin rapid release of remaining dpa. major conclusions from this work with b ... | 2015 | 25583976 |
| propidium monoazide combined with real-time pcr for selective detection of viable staphylococcus aureus in milk powder and meat products. | staphylococcus aureus is a spherical, gram-positive, pathogenic bacterium commonly associated with bovine mastitis and clinical infections. it is also recognized as a pathogen that causes outbreaks of food poisoning. the objective of this study was to develop and evaluate a rapid and reliable technique that combines propidium monoazide (pma) staining with real-time quantitative (q)pcr to detect and quantify viable cells of staph. aureus in milk powder and meat products. the inclusivity and exclu ... | 2015 | 25582587 |
| morphological and mechanical imaging of bacillus cereus spore formation at the nanoscale. | bacteria from the genus bacillus are able to transform into metabolically dormant states called (endo) spores in response to nutrient deprivation and other harsh conditions. these morphologically distinct spores are fascinating constructs, amongst the most durable cells in nature, and have attracted attention owing to their relevance in food-related illnesses and bioterrorism. observing the course of bacterial spore formation (sporulation) spatially, temporally and mechanically, from the vegetat ... | 2015 | 25581787 |
| evaluation of haccp system implementation on the quality of mixed fresh-cut salad prepared in a university canteen: a case study. | the increasing awareness that foods can represent vehicles for health risk factors has caused scientists and public authorities to multiply their efforts to reduce these risks to within acceptable limits. nevertheless, some challenging issues still remain unsolved and new ones have recently emerged, such as the increase in outbreaks of foodborne diseases originating from the consumption of meals at catering facilities. the study described in this article was aimed at evaluating the microbiologic ... | 2015 | 25619040 |
| plasmids are vectors for redundant chromosomal genes in the bacillus cereus group. | prokaryotic plasmids have played significant roles in the evolution of bacterial genomes and have a great impact on the metabolic functions of the host cell. many bacterial strains contain multiple plasmids, but the relationships between bacterial plasmids and chromosomes are unclear. we focused on plasmids from the bacillus cereus group because most strains contain several plasmids. | 2015 | 25608745 |
| squid pen chitin chitooligomers as food colorants absorbers. | one of the most promising applications of chitosanase is the conversion of chitinous biowaste into bioactive chitooligomers (cos). tku033 chitosanase was induced from squid pen powder (spp)-containing bacillus cereus tku033 medium and purified by ammonium sulfate precipitation and column chromatography. the enzyme was relatively more thermostable in the presence of the substrate and had an activity of 93% at 50 °c in a ph 5 buffer solution for 60 min. furthermore, the enzyme used for the cos pre ... | 2015 | 25608726 |
| food-grade antimicrobials potentiate the antibacterial activity of 1,2-hexanediol. | preservative agents determining the shelf life of cosmetic products must have effective antimicrobial activity while meeting safety requirements for topical use. in this study, we determined the antimicrobial activity of 1,2-hexanediol against several gram-positive and gram-negative bacteria. antimicrobial susceptibility tests have shown that 1,2-hexanediol exhibits broad-spectrum activity against gram-positive and gram-negative bacteria with mics of 0·5-2% (v/v). the bactericidal concentration ... | 2015 | 25631558 |
| analysis of bacillus cereus toxicity using pcr, elisa and a lateral flow device. | the aim of this study was to evaluate the performance of immunodetection methods and pcr analysis of enterotoxigenic bacillus cereus strains. | 2015 | 25627167 |
| improved in vitro assay for determining the mucin adherence of bacteria sensitive to triton x-100 treatment. | mucin-associated microbiota are in relatively close contact with the intestinal epithelium and may thus have a more pronounced effect on host health. we have previously developed a simple mucin agar assay to simulate initial mucus colonization by intestinal microbial communities. adherence of microbiota was estimated using flow cytometry after detachment with triton x-100. in this study, the effect of this detergent on the cultivability of both virulent and commensal strains was investigated. mu ... | 2015 | 25702162 |
| comparative analysis of biofilm formation by bacillus cereus reference strains and undomesticated food isolates and the effect of free iron. | biofilm formation of bacillus cereus reference strains atcc 14579 and atcc 10987 and 21 undomesticated food isolates was studied on polystyrene and stainless steel as contact surfaces. for all strains, the biofilm forming capacity was significantly enhanced when in contact with stainless steel (ss) as a surface as compared to polystyrene (ps). for a selection of strains, the total cfu and spore counts in biofilms were determined and showed a good correlation between cfu counts and total biomass ... | 2015 | 25700364 |
| polydiacetylene nanovesicles as carriers of natural phenylpropanoids for creating antimicrobial food-contact surfaces. | the ultimate goal of this study was developing antimicrobial food-contact materials based on natural phenolic compounds using nanotechnological approaches. among the methyl-β-cyclodextrin-encapsulated phenolics tested, curcumin showed by far the highest activity toward escherichia coli with a minimum inhibitory concentration of 0.4 mm. curcumin was enclosed in liposome-type polydiacetylene/phosholipid nanovesicles supplemented with n-hydroxysuccinimide and glucose. the fluorescence spectrum of t ... | 2015 | 25697369 |
| the length of a lantibiotic hinge region has profound influence on antimicrobial activity and host specificity. | lantibiotics are ribosomally synthesized (methyl)lanthionine containing peptides which can efficiently inhibit the growth of gram-positive bacteria. as lantibiotics kill bacteria efficiently and resistance to them is difficult to be obtained, they have the potential to be used in many applications, e.g., in pharmaceutical industry or food industry. nisin can inhibit the growth of gram-positive bacteria by binding to lipid ii and by making pores in their membrane. the c-terminal part of nisin is ... | 2015 | 25688235 |
| structure analysis of bacillus cereus mepr-like transcription regulator, bc0657, in complex with pseudo-ligand molecules. | the marr family of transcriptional regulatory proteins in bacteria and archaea respond to environmental changes and regulate transcriptional processes by ligand binding or cysteine oxidation. mepr belongs to the marr family, and its mutations are associated with the development of multidrug resistances, causing a growing health problem. therefore, it has been of great interest to locate the ligand binding site of mepr and reveal the ligand-mediated transcriptional regulation mechanism. here, we ... | 2015 | 25684184 |
| isolation and characterization of glacier vmy22, a novel lytic cold-active bacteriophage of bacillus cereus. | as a unique ecological system with low temperature and low nutrient levels, glaciers are considered a "living fossil" for the research of evolution. in this work, a lytic cold-active bacteriophage designated vmy22 against bacillus cereus myb41-22 was isolated from mingyong glacier in china, and its characteristics were studied. electron microscopy revealed that vmy22 has an icosahedral head (59.2 nm in length, 31.9 nm in width) and a tail (43.2 nm in length). bacteriophage vmy22 was classified a ... | 2015 | 25680445 |
| pyrosequencing analysis of microbial community and food-borne bacteria on restaurant cutting boards collected in seri kembangan, malaysia, and their correlation with grades of food premises. | this study adopts the pyrosequencing technique to identify bacteria present on 26 kitchen cutting boards collected from different grades of food premises around seri kembangan, a city in malaysia. pyrosequencing generated 452,401 of total reads of otus with an average of 1.4×10(7) bacterial cells/cm(2). proteobacteria, firmicutes and bacteroides were identified as the most abundant phyla in the samples. taxonomic richness was generally high with >1000 operational taxonomic units (otus) observed ... | 2015 | 25679309 |
| developing novel bacterial based bioformulation having pgpr properties for enhanced production of agricultural crops. | plant growth promoting rhizobacteria (pgpr) are beneficial rhizobacteria which enhance plant growth as well as the productivity by a variety of mechanisms pgpr were isolated from the rhizosphere region of som plants (machilus bombycina king) maintained at the central muga eri research and training institute, lahdoigarh, jorhat. a bacterial based bioformulation was prepared and sprayed over the experimental crops including tomato (solanum lycopersicum), cauliflower (brassica oleracea var botrytis ... | 2015 | 25675713 |
| purification and identification of an antibacterial protein from the symbiotic bacteria associated with novel entomopathogenic nematode, rhabditis (oscheius) sp. | entomopathogenic nematodes (epn) belonging to the families steinernematidae and heterorhabditidae and their symbiotic bacteria xenorhabdus and photorhabdus are well-known as biological control agents and are found to produce a wide range of bioactive secondary metabolites. studies carried out at the central tuber crops research institute (ctcri) on entomopathogenic nematodes resulted in the identification of novel epn belonging to the family rhabditidae. this study reports the purification of a ... | 2015 | 25666178 |
| chemodiversity of cereulide, the emetic toxin of bacillus cereus. | food-borne intoxications are increasingly caused by the dodecadepsipeptide cereulide, the emetic toxin produced by bacillus cereus. as such intoxications pose a health risk to humans, a more detailed understanding on the chemodiversity of this toxin is mandatory for the reliable risk assessment of b. cereus toxins in foods. mass spectrometric screening now shows a series of at least 18 cereulide variants, among which the previously unknown isocereulides a-g were determined for the first time by ... | 2015 | 25665710 |
| pathogenicity and characterization of a novel bacillus cereus sensu lato isolate toxic to the mediterranean fruit fly ceratitis capitata wied. | the lethal and sub-lethal effects of sporulated cultures of a novel bacillus cereus sensu lato strain lacking detectable cry genes and identified through morphological and genetic analyses, have been studied on the mediterranean fruit fly ceratitis capitata. the lethal effects on young larvae were concentration dependent, with a median lethal concentration (lc50) of 4.48 × 10(8)spores/g of diet. sporulated cultures of this strain significantly extended development time and reduced immature survi ... | 2015 | 25659732 |
| bacillus cereus bacteraemia in a patient of acute myeloid leukaemia. | 2015 | 25657148 | |
| chemical composition and antibacterial activities of seven eucalyptus species essential oils leaves. | in this paper, we have studied the essential oils chemical composition of the leaves of seven eucalyptus species developed in tunisia. eucalyptus leaves were picked from trees growing in different arboretums in tunisia. choucha and mrifeg arboretums located in sedjnene, region of bizerte (choucha: e. maideni, e. astrengens et e. cinerea; mrifeg : e. leucoxylon), korbous arboretums located in the region of nabeul, north east tunisia with sub-humid bioclimate, (e. lehmani), souiniet-ain drahem arb ... | 2015 | 25654423 |
| bioactive prenylated xanthones from the young fruits and flowers of garcinia cowa. | five new xanthones, garciniacowones a-e (1-5), together with 14 known xanthones, 6-19, were isolated from the young fruits and fresh flowers of garcinia cowa. the structures of 1-5 were elucidated by analysis of their 1d and 2d nmr spectra and mass spectrometric data. the compounds 1-19 were tested in vitro for their antimicrobial activity and for their ability to inhibit α-glucosidase. compounds 16 and 17 showed the most potent α-glucosidase inhibitory activity, with ic50 values of 7.8 ± 0.5 an ... | 2015 | 25651042 |
| evaluation of antioxidant, total phenol and flavonoid content and antimicrobial activities of artocarpus altilis (breadfruit) of underutilized tropical fruit extracts. | artocarpus altilis (breadfruit) pulp, peel and whole fruit were extracted with various solvents such as hexane, dichloromethane (dcm) and methanol. the antioxidant activity of these extracts were examined using the stable 2,2-diphenyl-1-picrylhydrazyl (dpph) radical scavenging test. ic50 was 55 ± 5.89 μg/ml for the pulp part of methanol extract. in the β-carotene bleaching assay, the antioxidant activity was 90.02 ± 1.51 % for the positive control (trolox) and 88.34 ± 1.31 % for the pulp part of ... | 2015 | 25649443 |
| microbial diversity of mangrove sediment in shenzhen bay and gene cloning, characterization of an isolated phytase-producing strain of spc09 b. cereus. | phytases hydrolyze phytate to release inorganic phosphate, which decreases the requirement for phosphorus in fertilizers for crops and thus reduces environmental pollutants. this study analyzed microbial communities in rhizosphere sediment, collected in september 2012 from shenzhen bay, guangdong, china, using high-throughput pyrosequencing; the results showed that the dominant taxonomic phyla were chloroflexi, firmicutes, and proteobacteria, and the proportion of the beneficial bacteria, bacill ... | 2015 | 25646962 |
| characterisation of antimicrobial extracts from dandelion root (taraxacum officinale) using lc-spe-nmr. | plant extracts have traditionally been used as sources of natural antimicrobial compounds, although in many cases, the compounds responsible for their antimicrobial efficacy have not been identified. in this study, crude and dialysed extracts from dandelion root (taraxacum officinale) were evaluated for their antimicrobial properties against gram positive and gram negative bacterial strains. the methanol hydrophobic crude extract (dre3) demonstrated the strongest inhibition of microbial growth a ... | 2015 | 25644491 |
| one-day pulsed-field gel electrophoresis protocol for rapid determination of emetic bacillus cereus isolates. | bacillus cereus, the gram-positive and spore-forming ubiquitous bacterium, may cause emesis as the result of food intoxication with cereulide, a heat-stable emetic toxin. rapid determination of cereulide-positive b. cereus isolates is of highest importance due to consequences of this intoxication for human health and life. here we present a 1-day pulsed-field gel electrophoresis for emetic b. cereus isolates, which allows rapid and efficient determination of their genomic relatedness and helps d ... | 2015 | 25639850 |
| protein structure. structure and activity of tryptophan-rich tspo proteins. | translocator proteins (tspos) bind steroids and porphyrins, and they are implicated in many human diseases, for which they serve as biomarkers and therapeutic targets. tspos have tryptophan-rich sequences that are highly conserved from bacteria to mammals. here we report crystal structures for bacillus cereus tspo (bctspo) down to 1.7 å resolution, including a complex with the benzodiazepine-like inhibitor pk11195. we also describe bctspo-mediated protoporphyrin ix (ppix) reactions, including ca ... | 2015 | 25635100 |
| spore prevalence and toxigenicity of bacillus cereus and bacillus thuringiensis isolates from u.s. retail spices. | recent incidents of foodborne illness associated with spices as the vehicle of transmission prompted this examination of u.s. retail spices with regard to bacillus cereus. this study focused on the levels of aerobic-mesophilic spore-forming bacteria and b cereus spores associated with 247 retail spices purchased from five states in the united states. samples contained a wide range of aerobic-mesophilic bacterial spore counts (< 200 to 8.3 × 10(7) cfu/g), with 19.1% of samples at levels above 10( ... | 2015 | 25719886 |
| thiopeptide antibiotics stimulate biofilm formation in bacillus subtilis. | bacteria have evolved the ability to produce a wide range of structurally complex natural products historically called "secondary" metabolites. although some of these compounds have been identified as bacterial communication cues, more frequently natural products are scrutinized for antibiotic activities that are relevant to human health. however, there has been little regard for how these compounds might otherwise impact the physiology of neighboring microbes present in complex communities. bac ... | 2015 | 25713360 |
| catalytic and thermodynamic properties of glycosylated bacillus cereus cyclodextrin glycosyltransferase. | cyclodextrin glycosyltransferase (cgtase) was covalently coupled to five oxidized polysaccharides differing in structure and chemical nature. the conjugates were evaluated for the retained activity, kinetic and thermodynamic stability. the conjugated cgtase with oxidized dextran (mw 47000) had the highest retained specific activity (70.05%) and the highest half-life (t1/2) at 80°c. compared to the native enzyme, the conjugated preparation exhibited higher optimum temperature, lower activation en ... | 2015 | 25709024 |
| mechanical and antibacterial properties of novel high performance chitosan/nanocomposite films. | zinc oxide (zno) nanoparticle was successfully synthesized using hydrothermal method as well as, silver (ag) nanoparticle was direct prepared during chitosan nanocomposites preparation. chitosan films were investigated by recognized the effect of kinds of acids (formic or acetic acid). correspondingly, using of concentrated naoh was checking for facilitating de-casting of the films. utilization of formic acid arisen higher quality films than those films produced using acetic acid as solvent. opt ... | 2015 | 25709016 |
| antibacterial, antifungal and in vitro antileukaemia activity of metal complexes with thiosemicarbazones. | 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone 4-ethyl-thiosemicarbazone (hl) and its copper(ii), vanadium(v) and nickel(ii) complexes: [cu(l)(cl)]·c₂h₅oh·(1), [cu(l)₂]·h₂o (2), [cu(l)(br)]·h₂o·ch₃oh (3), [cu(l)(no₃)]·2c₂h₅oh (4), [vo₂(l)]·2h₂o (5), [ni(l)₂]·h₂o (6), were synthesized and characterized. the ligand has been characterized by elemental analyses, ir, (1) h nmr and (13) c nmr spectroscopy. the tridentate nature of the ligand is evident from the ir spectra. the copper(ii), vanadium(v) and ni ... | 2015 | 25708540 |
| isolation of bacillus cereus group from the fecal material of endangered wood turtles. | members of the bacillus cereus group are opportunistic human pathogens. they can be found in a broad range of foods. diarrheal food poisoning and/or emetic type syndromes can result from eating contaminated food. in this study, seven b. cereus group members were isolated from the fecal material of wood turtles (glyptemys insculpta). the isolates were then assessed for the presence of enterotoxin genes (nhea, entfm, hblc, and cytk) using pcr. the most prevalent is the nonhemolytic enterotoxin gen ... | 2015 | 26175111 |
| antibacterial δ(1) -3-ketosteroids from the south china sea gorgonian coral subergorgia rubra. | three new δ(1) -3-ketosteroids characterized with a 9-oh, subergosterones a-c (1-3), together with five known analogs 4-8, were obtained from the gorgonian coral subergorgia rubra collected from the south china sea. the structures of 1-3, including their absolute configurations, were determined by comprehensive spectroscopic methods and electronic circular dichroism (ecd) experiments. compounds 2 and 3 exhibited inhibitory antibacterial activities against bacillus cereus with mic values of 1.56 ... | 2015 | 26172327 |
| in vitro antibacterial activities and mechanism of sugar fatty acid esters against five food-related bacteria. | the objective of this study was to evaluate the antibacterial activities of sugar fatty acid esters, with different fatty acid and saccharide moieties, against five food-related bacteria including bacillus cereus, bacillus subtilis, staphylococcus aureus, escherichia coli and salmonella typhimurium. sucrose monocaprate showed the strongest antibacterial activity against all tested bacteria, especially gram-positive bacteria. the minimum inhibitory concentrations (mics) for gram-positive bacteria ... | 2015 | 25977039 |
| assessment of the microbial load of some medicinal plants commonly used in romania. | this study aims to assess the microbial load of some medicinal plants (matricaria chamomilla, achillea millefolium, ocimum basilicum, calendula officinalis, tilia cordata, hypericum perforatum) commonly used as medicinal teas. | 2015 | 25970977 |
| antiplatelet and antithrombotic activity of a fibrin(ogen)olytic protease from bacillus cereus strain ff01. | fibrin(ogen)olytic enzymes offer great promise for the treatment of thrombosis associated disorders. the present study describes the characterization of an extracellular fibrin(ogen)olytic serine protease (named bacethrombase) purified from the bacillus cereus strain ff01. the molecular mass of the bacethrombase was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and matrix assisted laser desorption/ionization-time-of-flight-mass spectroscopy analyses at 39.5 kda and 38,4 ... | 2015 | 25964180 |
| time dynamics of the bacillus cereus exoproteome are shaped by cellular oxidation. | at low density, bacillus cereus cells release a large variety of proteins into the extracellular medium when cultivated in ph-regulated, glucose-containing minimal medium, either in the presence or absence of oxygen. the majority of these exoproteins are putative virulence factors, including toxin-related proteins. here, b. cereus exoproteome time courses were monitored by nanolc-ms/ms under low-oxidoreduction potential (orp) anaerobiosis, high-orp anaerobiosis, and aerobiosis, with a specific f ... | 2015 | 25954265 |
| synthesis and antibacterial properties of 2,3-dideoxyglucosides of terpene alcohols and phenols. | essential oils and their oxygenated terpene constituents possess potent antimicrobial properties. in the present study, a facile synthetic route to the 2,3-dideoxy 1-o-glucosides of important phenols and terpene alcohols in excellent yields (85-96%) has been delineated. studies on their antimicrobial action against four food-borne pathogens--bacillus cereus, staphylococcus aureus, escherichia coli and yersinia enterocolitica--demonstrated that the zone of inhibition, in general, was higher for t ... | 2015 | 25952857 |
| isolation of lactic acid bacteria showing antioxidative and probiotic activities from kimchi and infant feces. | the purpose of this study was to investigate lactic acid bacteria with antioxidative and probiotic activities isolated from korean healthy infant feces and kimchi. isolates a1, a2, s1, s2, and s3 were assigned to lactobacillus sp. and isolates a3, a4, e1, e2, e3, and e4 were assigned to leuconostoc sp. on the basis of their physiological properties and 16s ribosomal dna sequence analysis. most strains were confirmed as safe bioresources through nonhemolytic activities and non-production of harmf ... | 2015 | 25951843 |
| decontamination of mesquite pod flour naturally contaminated with bacillus cereus and formation of furan by ionizing irradiation. | mesquite pod flour produced from nitrogen-fixing trees of the prosopis species has a unique aroma and flavor that is preferred by some consumers. due to the presence of wildlife, grazing domestic animals, and insects, the pods have a high potential of being contaminated with human pathogenic bacteria, such as bacillus cereus. nonthermal processing technologies are helpful to reduce the population of microorganisms in the flour because heating deteriorates the characteristic flavor. a study was c ... | 2015 | 25951390 |
| incidence, antibiotic susceptibility, and toxin profiles of bacillus cereus sensu lato isolated from korean fermented soybean products. | korean fermented soybean products, such as doenjang, kochujang, ssamjang, and cho-kochujang, can harbor foodborne pathogens such as bacillus cereus sensu lato (b. cereus sensu lato). the aim of this study was to characterize the toxin gene profiles, biochemical characteristics, and antibiotic resistance patterns of b. cereus sensu lato strains isolated from korean fermented soybean products. eighty-eight samples of korean fermented soybean products purchased from retails in seoul were tested. th ... | 2015 | 25950845 |
| effect of high hydrostatic pressure combined with moderate heat to inactivate pressure-resistant bacteria in water-boiled salted duck. | the objective of this work was to study the effect of high hydrostatic pressure combined with moderate heat to inactivate pressure-resistant bacteria in water-boiled salted duck meat (wbsdm), and to establish suitable procedures to improve the quality of wbsdm. the conditions (300 mpa/60 °c, 400 mpa/60 °c, and 500 mpa/50 °c) effectively inactivated the pressure-resistant bacteria (bacillus cereus and staphylococcus warneri) in wbsdm. although more pressure-resistant than s. warneri, the above tr ... | 2015 | 25943207 |
| bactericidal mechanism of bio-oil obtained from fast pyrolysis of pinus densiflora against two foodborne pathogens, bacillus cereus and listeria monocytogenes. | foodborne bacteria are the leading cause of food spoilage and other related diseases. in the present study, the antibacterial activity of bio-oil (bo) manufactured by fast pyrolysis of pinewood sawdust (pinus densiflora siebold and zucc.) against two disease-causing foodborne pathogens (bacillus cereus and listeria monocytogenes) was evaluated. bo at a concentration of 1000 μg/disc was highly active against both b. cereus (10.0-10.6 mm-inhibition zone) and l. monocytogenes (10.6-12.0-mm inhibiti ... | 2015 | 25928035 |
| effects of ripening duration and rosemary powder addition on salchichon modified sausage quality. | the ripening durations and ingredients for the salchichon sausages were modified to increase pork rear leg consumption by korean consumers. the salchichon, a ripened pork sausage, was produced to evaluate the efficacy of two different ripening durations with and without rosemary powder on salchichon sausage quality, and the treatments were: i) 45 days of ripening without rosemary, ii) 60 days of ripening without rosemary, iii) 45 days of ripening with 0.05% rosemary, and iv) 60 days of ripening ... | 2015 | 25924959 |
| the water cycle, a potential source of the bacterial pathogen bacillus cereus. | the behaviour of the sporulating soil-dwelling bacillus cereus sensu lato (b. cereus sl) which includes foodborne pathogenic strains has been extensively studied in relation to its various animal hosts. the aim of this environmental study was to investigate the water compartments (rain and soil water, as well as groundwater) closely linked to the primary b. cereus sl reservoir, for which available data are limited. b. cereus sl was present, primarily as spores, in all of the tested compartments ... | 2015 | 25918712 |
| correction: capsules, toxins and atxa as virulence factors of emerging bacillus cereus biovar anthracis. | [this corrects the article doi: 10.1371/journal.pntd.0003455.]. | 2015 | 25902046 |
| production, optimization and characterization of polyhydroxybutyrate, a biodegradable plastic by bacillus spp. | poly-β-hydroxybutyrate (phb) is the intracellular lipid reserve accumulated by many bacteria. the most potent terrestrial bacterium bacillus cereus se-1 showed more phb accumulating cells (22.1 and 40% after 48 and 72 h) than that of the marine bacillus sp. cs-605 (5 and 33% after 48 and 72 h). both the isolates harbored phbb gene and the characteristics c=o peak was observed in the extracted phb by fourier transformed infrared spectroscopy analysis. maltose was found to be the most suitable car ... | 2015 | 25900381 |
| challenge study of the pathogen reduction capacity of the theraflex mb-plasma technology. | although most pathogen reduction systems for plasma primarily target viruses, bacterial contamination may also occur. this study aimed to investigate the bacterial reduction capacity of a methylene blue (mb) treatment process and its virus inactivation capacity in lipaemic plasma. | 2015 | 25899343 |
| evaluation of the effectiveness of a pathogen inactivation technology against clinically relevant transfusion-transmitted bacterial strains. | to increase blood safety, various procedures are currently implemented, including donor selection, optimized donor arm disinfection, and diversion. in addition, pathogen inactivation (pi) techniques can be used for platelets (plts) and plasma concentrates. | 2015 | 26013691 |
| depsipeptide intermediates interrogate proposed biosynthesis of cereulide, the emetic toxin of bacillus cereus. | cereulide and isocereulides a-g are biosynthesized as emetic toxins by bacillus cereus via a non-ribosomal peptide synthetase (nrps) called ces. although a thiotemplate mechanisms involving cyclo-trimerization of ready-made d-o-leu-d-ala-l-o-val-l-val via a thioesterase (te) domain is proposed for cereulide biosynthesis, the exact mechanism is far from being understood. uplc-tof ms analysis of b. cereus strains in combination with (13)c-labeling experiments now revealed tetra-, octa-, and dodeca ... | 2015 | 26013201 |
| design, synthesis, in silico and in vitro studies of substituted 1, 2, 3, 4- tetrahydro pyrimidine phosphorus derivatives. | molecular docking studies of the designed two series (4a-l, 6a-l, 9 and 10) of novel substituted phosphorylated 1, 4-dihydropyridine and 1,2,3,4-tetrahydropyrimidine derivatives against the drug targets of dhfr from bacillus cereus, lpxc from pseudomonas aeruginosa, idh from e. coli and murb from staphylococcus aureus were encouraged for their synthesis. these compounds were synthesized from substituted aromatic aldehydes, thiourea/urea and ethyl acetoacetate in the presence of polyphosphoric ac ... | 2015 | 26004048 |
| antibacterial activity of native california medicinal plant extracts isolated from rhamnus californica and umbellularia californica. | antimicrobial resistance (amr) is a major threat to global public health. medicinal plants have long been used as remedies for infectious diseases by native cultures around the world and have the potential for providing effective treatments for antibiotic-resistant infections. rhamnus californica (rhamnaceae) and umbellularia californica (lauraceae) are two indigenous california plant species historically used by native americans to treat skin, respiratory and gastrointestinal infections. this s ... | 2015 | 26001558 |
| cereulide food toxin, beta cell function and diabetes: facts and hypotheses. | the incidence of both type 1 and type 2 diabetes is increasing and although environmental pollutants are believed to be potential culprits, the extent to which they can be held responsible remains uncertain. some bacterial strains of the bacillus cereus produce a toxin, cereulide, which is frequently found in starchy meals and which is difficult to eradicate from the food chain as it is highly resistant to heat, acidity and proteolysis. while cereulide is well known to cause acute emetic toxicit ... | 2015 | 25998918 |
| [identification of hexavalent chromium reducing bacteria cr4-1 and optimization of its reduction conditions]. | to identify the hexavalent chromium reduction cr4-1, and to study the better conditions of the bacterial growth and its cr(vi) reduction. | 2015 | 25997219 |
| involvement of the cask/r two-component system in optimal unsaturation of the bacillus cereus fatty acids during low-temperature growth. | bacillus cereus sensu lato is composed of a set of ubiquitous strains including human pathogens that can survive a range of food processing conditions, grow in refrigerated food, and sometimes cause food poisoning. we previously identified the two-component system cask/r that plays a key role in cold adaptation. to better understand the cask/r-controlled mechanisms that support low-temperature adaptation, we performed a transcriptomic analysis on the atcc 14579 strain and its isogenic ∆cask/r mu ... | 2015 | 25987542 |
| selenium-fortified wheat: potential of microbes for biofortification of selenium and other essential nutrients. | selenium (se) is an essential micronutrient for humans and animals, and se deficiency is a worldwide problem. plants are a main dietary source of se for humans and livestock. in this study we investigated the effect of two selenium-tolerant bacterial strains bacillus cereus-yap6 and bacillus licheniformis-yap7, on the growth and se uptake by wheat plants. the bacteria-inoculated plants exhibited a significant increase in spike length, shoot length and dry biomass. inoculated se-treated plants al ... | 2015 | 26030365 |
| supercritical co2 extraction of functional compounds from spirulina and their biological activity. | supercritical carbon dioxide (scco2) extraction and fractionation of spirulina platensis was carried out to obtain functional compounds with antioxidant, antimicrobial and enzyme inhibitory activities. extraction of scco2 was carried out using 200 g of spirulina powder at 40 ºc under 120 bar pressure with co2 flow rate of 1.2 kg h(-1). scco2 fraction obtained was further treated with hexane and ethyl acetate to identify its components. individual components were identified by comparing mass spec ... | 2015 | 26028745 |
| [effect of bacillus cereus hemolysin ii on hepatocyte cells]. | we investigated the efficiency of increasing the permeability (permeabilization) of cell membranes in primary liver cells by bacillus cereus hemolysin ii. an assessment of the degree of permeabilization was car ried out by measuring the fluorescence intensity of various low molecular weight dyes, which enter through pores into hepatocyte cells cultivated with hemolysin. we uncovered a high efficacy of hemolysin hlyii action on hepatocyte cell walls, which exceeded the effect of nonionic detergen ... | 2015 | 26027363 |
| sonodynamic action of curcumin on foodborne bacteria bacillus cereus and escherichia coli. | bacterial contamination is an important cause of foodborne diseases. the present study aimed to investigate sonodynamic action of curcumin on foodborne bacteria bacillus cereus (b. cereus) and escherichia coli (e. coli). the uptake of curcumin was measured for optimizing the concentration incubation time before ultrasound sonication, and colony forming units (cfu) were counted after ultrasound treatment. the chromosomal dna fragmentation of bacteria was analyzed and the effect of hypoxic conditi ... | 2015 | 26026869 |
| improvement of glycine oxidase by dna shuffling, and site-saturation mutagenesis of f247 residue. | glyphosate is a broad spectrum herbicide widely used throughout the world, and it could be degraded by glycine oxidase (go) through cn bond cleavage. for a better understanding of the structure-function relationship and improving the activity of b3s1 (go from bacillus cereus), dna shuffling was performed. a mutant b4s7 (the km, vmax, kcat and kcat/km values on glyphosate were 0.1 mm, 0.002401 mm min(-1), 3.62 min(-1) and 36.2 mm(-1) min(-1), respectively. the four parameters on glycine were 50.3 ... | 2015 | 26025077 |
| bioactive compounds isolated from submerged fermentations of the chilean fungus stereum rameale. | liquid fermentations of the fungus stereum rameale (n° 2511) yielded extracts with antibacterial activity. the antibacterial activity reached its peak after 216 h of stirring. bioassay-guided fractionation methods were employed for the isolation of the bioactive metabolites. three known compounds were identified: ms-3 (1), vibralactone (2) and vibralactone b (3). the three compounds showed antibacterial activity as a function of their concentration. minimal bactericidal concentrations (mbc) of c ... | 2015 | 26020559 |
| optimization and purification of mannanase produced by an alkaliphilic-thermotolerant bacillus cereus n1 isolated from bani salama lake in wadi el-natron. | an alkaliphilic-thermotolerant bacillus cereus n1 isolated from bani salama lake, wadi el-natron, egypt, was proved to produce mannanase enzyme. optimization of the fermentation medium components using plackett-burman design was applied. glucose and inoculum size were found to be the most significant factors enhancing the production of the enzyme. on applying optimized medium in the fermentation process, an enzyme productivity of 42.2 uml(-1) was achieved with 6.4 fold increase compared to the b ... | 2015 | 26019646 |
| antimicrobial activity and antibiotic susceptibility of lactobacillus and bifidobacterium spp. intended for use as starter and probiotic cultures. | antimicrobial activity and antibiotic susceptibility were tested for 23 lactobacillus and three bifidobacterium strains isolated from different ecological niches. agar-well diffusion method was used to test the antagonistic effect (against staphylococcus aureus, escherichia coli, bacillus cereus and candida albicans) of acid and neutralized (ph 5.5) lyophilized concentrated supernatants (cell-free supernatant; cfs) and whey (cell-free whey fractions; cfw) from de man-rogosa-sharpe/trypticase-phy ... | 2015 | 26019620 |
| forensic differentiation of bacillus cereus spores grown using different culture media using raman spectroscopy. | some microorganisms have been shown to retain a chemical signature indicative of the medium used for culturing. however, the repeatability of medium-specific chemical signatures has not been demonstrated from samples of microorganisms produced in the same batch or in different batches by the same sporulation protocol. here, the variation in raman spectra of bacterial endospores repeatedly prepared by the same procedure is compared to the variation between raman spectra of spores prepared using d ... | 2015 | 25893804 |
| bacillus cereus food poisoning: international and indian perspective. | food borne illnesses result from eating food or drinking beverages that are contaminated with chemical matter, heavy metals, parasites, fungi, viruses and bacteria. bacillus cereus is one of the food-borne disease causing bacteria. species of bacillus and related genera have long been troublesome to food producers on account of their resistant endospores. their spores may be present on various types of raw and cooked foods, and their ability to survive high cooking temperatures requires that coo ... | 2015 | 25892750 |
| unistatus - a cross-sectional study on the contamination of uniforms in the danish ambulance service. | patients are at risk of contracting infections due to the presence of disease-causing microorganisms that can be transmitted from the medical staff's uniforms to the patient. the dual purpose of this study was to examine the contamination level of the uniforms worn by ambulance staff after a shift and to test the effect of washing of the uniform with and without a detergent containing acetic peroxide. | 2015 | 25889860 |
| antimicrobial and immunomodulatory efficacy of extracellularly synthesized silver and gold nanoparticles by a novel phosphate solubilizing fungus bipolaris tetramera. | particulates of nanometers size have occupied a significant area in the field of medicinal and agricultural purposes due to their large surface-to-volume ratio and exceptional physicochemical, electronic and mechanical properties. myconanotechnology, an interface between mycology and nanotechnology is budding nowadays for nanoparticle-fabrication using fungus or its metabolites. in the present study, we have isolated and characterized a novel phosphate solubilizing fungus b. tetramera kf934408 f ... | 2015 | 25881309 |