Publications

[penicillin v a first choice in infected cat bites].		2009	19496253
comparison of polymerase chain reaction primer sets for amplification of rodent pasteurellaceae.	monitoring of rodents for pasteurellaceae infection may be carried out by the polymerase chain reaction (pcr). we tested which of 17 rodent pasteurellaceae strains were detected by three pcr primer sets. by phylogenetic analysis, 12 strains were assigned to the rodent cluster and five strains to other clusters, namely the somnus cluster, pasteurella sensu stricto, actinobacillus sensu stricto, the mannheimia and rossii cluster. a primer set developed to detect biotype heyl [pasteurella] pneumotr ...	2009	19505934
[overwhelming postsplenectomy infection 22 years after splenectomy].	a 25-year-old man undergoing splenectomy at 3 years of age to treat idiopathic thrombocytopenic purpura but no history of streptococcus pneumonia vaccination, and reporting high fever, nausea, and headache developed purpura, confusion, and hypotension the next day and was admitted. detailed examination showed disseminated intravascular coagulation and multiple-organ dysfunction. chest x-ray and computed tomography (ct) showed pneumonia and pleural effusion. blood culture was positive for s. pneu ...	2009	19522311
comparative studies on [pasteurella] testudinis and [pasteurella] testudinis-like bacteria and proposal of chelonobacter oris gen. nov., sp. nov. as a new member of the pasteurellaceae.	a collection of 12 strains isolated from diseased tortoises tentatively identified as [pasteurella] testudinis-like based on phenotypical characters were compared with three reference strains of [pasteurella] testudinis. all strains could be separated from the reference strains with respect to 16s rrna gene sequence, partial sequence of the house-keeping gene rpob and by phenotypic characters. a new genus of pasteurellaceae pohl 1981 with one species chelonobacter oris gen. nov., sp. nov. is pro ...	2009	19528199
multiresistance in pasteurella multocida is mediated by coexistence of small plasmids.	in most gram-negative bacteria, acquired multiresistance is conferred by large plasmids compiling numerous antimicrobial resistance genes. here, we show an evolutionary alternative strategy used by pasteurella multocida to become resistant to multiple clinically relevant antibiotics. thirteen beta-lactam-resistant clinical isolates, concomitantly resistant to tetracyclines and/or streptomycin as well as to sulfonamides, were studied. pulsed-field gel electrophoresis analysis revealed different p ...	2009	19528282
bite-related and septic syndromes caused by cats and dogs.	bite infections can contain a mix of anaerobes and aerobes from the patient's skin and the animal's oral cavity, including species of pasteurella, streptococcus, fusobacterium, and capnocytophaga. domestic cat and dog bite wounds can produce substantial morbidity and often require specialised care techniques and specific antibiotic therapy. bite wounds can be complicated by sepsis. disseminated infections, particularly those caused by capnocytophaga canimorsus and pasteurella multocida, can lead ...	2009	19555903
lung pathology and infectious agents in fatal feedlot pneumonias and relationship with mortality, disease onset, and treatments.	this study charted 237 fatal cases of bovine respiratory disease (brd) observed from may 2002 to may 2003 in a single oklahoma feed yard. postmortem lung samples were used for agent identification and histopathology. late in the study, 94 skin samples (ear notches) were tested for bovine viral diarrhea virus (bvdv) by immunohistochemistry (ihc). bovine respiratory disease morbidity was 14.7%, and the mortality rate of all causes was 1.3%, with more than half (53.8%) attributed to brd (0.7% total ...	2009	19564494
phylogenetic relationships of unclassified, satellitic pasteurellaceae obtained from different species of birds as demonstrated by 16s rrna gene sequence comparison.	avian haemophili demonstrating in vitro satellitic growth, also referred to as the v-factor or nad requirement, have mainly been classified with avibacterium paragallinarum (haemophilus paragallinarum), avibacterium avium (pasteurella avium), avibacterium volantium (pasteurella volantium) and avibacterium sp. a (pasteurella species a). the aim of the present study was to assess the taxonomic position of 18 v-factor-requiring isolates of unclassified haemophilus-like organisms isolated from galli ...	2009	19573597
comparative studies on [pasteurella] testudinis and [p.] testudinis-like bacteria and proposal of chelonobacter oris gen. nov., sp. nov. as a new member of the family pasteurellaceae.	a collection of 12 strains, isolated from diseased tortoises and tentatively identified as [pasteurella] testudinis-like based on phenotypic characters, was compared with three reference strains of [p.] testudinis. all strains could be separated from the reference strains with respect to 16s rrna gene sequences, partial sequences of the rpob housekeeping gene and by phenotypic characters. based upon differences in 16s rrna and rpob gene sequences, the new isolates are suggested to represent a no ...	2009	19578148
intranasal vaccination of calves with mannheimia haemolytica chimeric protein containing the major surface epitope of outer membrane lipoprotein plpe, the neutralizing epitope of leukotoxin, and cholera toxin subunit b.	this study was done to determine if intranasal vaccination of weaned beef calves with a chimeric protein containing the immunodominant surface epitope of mannheimia haemolytica plpe (r2) and the neutralizing epitope of leukotoxin (nlkt) covalently linked to truncated cholera toxin (ct) subunit b (ctb) could stimulate secretory and systemic antibodies against m. haemolytica while enhancing resistance of cattle against m. haemolytica intrabronchial challenge. sixteen weaned beef calves were intran ...	2009	19581005
comparative analysis of pasteurella pneumotropica isolates from laboratory mice and rats.	selected biochemical and genetic characteristics of the wild-type strains of pasteurella pneumotropica isolated from mice and rats were investigated and compared in order to determine the significant differences among the isolates. the isolates were divided into six groups on the basis of the patterns of carbon source utilization in the host rodents. the genome sizes were determined by electrophoretic analysis, and the mean genome size of the isolates from mice was larger than that of the isolat ...	2009	19234758
identification of immunogenic proteins associated with protection against haemorrhagic septicaemia after vaccination of calves with a live-attenuated aroa derivative of pasteurella multocida b:2.	pasteurella multocida serotype b:2 is the causative agent of haemorrhagic septicaemia (hs), a fatal disease of cattle and buffaloes. as a step towards the identification of individual antigens that may protect against hs, proteins present in a sonicated cell extract (sce) and outer-membrane protein (omp) preparation of a wild-type p. multocida serotype b:2 were investigated by immunoblotting with sera from calves which had been protected against challenge with a virulent strain of p. multocida b ...	2009	19243798
expression, purification and crystallization of class c acid phosphatases from francisella tularensis and pasteurella multocida.	class c nonspecific acid phosphatases are bacterial enzymes that are secreted across the cytoplasmic membrane and hydrolyze a variety of phosphomonoesters at acidic ph. these enzymes are of interest for the development of improved vaccines and clinical diagnostic methods. in one case, the category a pathogen francisella tularensis, the class c phosphatase plays a role in bacterial fitness. here, the cloning, expression, purification and crystallization methods for the class c acid phosphatases f ...	2009	19255471
orbital abscess bacterial isolates and in vitro antimicrobial susceptibility patterns in dogs and cats.	to determine bacterial populations, in vitro antimicrobial susceptibility patterns, and sources of microorganisms for dogs and cats with orbital abscess.	2009	19261163
neonatal pasteurellosis: a review of reported cases.	pasteurellosis is an uncommon infectious disease in humans mainly caused by pasteurella multocida infection in neonates and has been rarely reported.	2009	19208671
antimicrobial properties of the equine alpha-defensin defa1 against bacterial horse pathogens.	defensins are small effector molecules of the innate immune system, synthesised by various organisms including plants and animals. the peptides act as endogenous antibiotics with an antimicrobial activity against a broad spectrum of microbes including bacteria, fungi and viruses. alpha-defensins are a subgroup of the defensin family, their synthesis is limited to some tissues and furthermore to some mammalian species including the horse. equine defa1 is an enteric alpha-defensin exclusively prod ...	2009	19211153
functions and regulatory mechanisms of gq-signaling pathways.	gq family members of heterotrimeric g protein activate beta isoforms of phospholipase c that hydrolyzes phosphatidylinositol phosphate to diacylglycerol and inositol trisphosphate, leading to the protein kinase c activation and intracellular ca(2+) mobilization, respectively. to understand the functions and regulatory mechanisms of gq-signaling pathways, we first introduce the galphaq-interacting proteins, which function as the effectors and the modulators of gq. next, we describe the pasteurell ...	2009	19212139
dog bite wound infection by pasteurella dagmatis misidentified as pasteurella pneumotropica by automated system vitek 2.		2009	19765934
structural and genetic basis for the serological differentiation of pasteurella multocida heddleston serotypes 2 and 5.	pasteurella multocida is classified into 16 serotypes according to the heddleston typing scheme. as part of a comprehensive study to define the structural and genetic basis of this scheme, we have determined the structure of the lipopolysaccharide (lps) produced by p. multocida strains m1404 (b:2) and p1702 (e:5), the type strains for serotypes 2 and 5, respectively. the only difference between the lps structures made by these two strains was the absence of a phosphoethanolamine (petn) moiety at ...	2009	19767423
is there still a benefit to operate appendiceal abscess on board french nuclear submarines?	appendicular abscess occurred in 14.2% of patients presenting acute appendicitis. management of these patients remains controversial, ranging from an emergency appendectomy to a nonoperative treatment. on board french nuclear submarines, the usual treatment for all cases of appendiceal masses, including both appendicitis and appendiceal abscess, is an appendectomy. in the past 5 years, the introduction of ultrasonography (us) on board has enabled the diagnosis of appendiceal abscess with a high ...	2009	19743747
what is your diagnosis?		2009	19751160
expression of 4 truncated fragments of pasteurella multocida toxin and their immunogenicity.	pasteurella multocida toxin (pmt) is a poor antigen that becomes more immunogenic after its native structure has been destroyed. in contrast, partially truncated pmt proteins, which are predicted to be good antigens when used as a vaccine, might be used to improve the control of atrophic rhinitis in pigs. in this study, 4 truncated pmt fragments were expressed in escherichia coli, and those 4 fragments were inoculated into mice to produce the polyclonal antibodies. the results of an enzyme-linke ...	2009	19794890
the effect of the macrolide antibiotic tylosin on microbial diversity in the canine small intestine as demonstrated by massive parallel 16s rrna gene sequencing.	recent studies have shown that the fecal microbiota is generally resilient to short-term antibiotic administration, but some bacterial taxa may remain depressed for several months. limited information is available about the effect of antimicrobials on small intestinal microbiota, an important contributor to gastrointestinal health. the antibiotic tylosin is often successfully used for the treatment of chronic diarrhea in dogs, but its exact mode of action and its effect on the intestinal microbi ...	2009	19799792
enterobacteriaceae and pseudomonadaceae on the dorsum of the human tongue.	the aim of this study was to correlate the presence of enterobacteriaceae, pseudomonadaceae, moraxellaceae and xanthomonadaceae on the posterior dorsum of the human tongue with the presence of tongue coating, gender, age, smoking habit and denture use.	2009	19936511
identification of riemerella anatipestifer genes differentially expressed in infected duck livers by the selective capture of transcribed sequences technique.	riemerella anatipestifer is the causative agent of duck septicaemia. determination of r. anatipestifer virulence mechanisms will help us to effectively control this contagious agent. the differentially expressed gene profile of r. anatipestifer in infected duck livers was therefore identified and compared with in vitro cultures by selective capture of transcribed sequences analysis. a total of 48 genes were identified, of which 43 were genes that encode enzymes for amino acid biosynthesis and me ...	2009	19937518
high prevalence of pseudomonas species in soil samples from ternate island-indonesia.	in the present study, ten soil samples were examined and the ph of the soil was recorded. for bacterial isolation, a sterile nutrient and blood agars were used. gram stain and biochemical tests were done for identification. a total of 384 genus were isolated, 314 (81.8%) were identified as pseudomonas species of which 245 (78.0%) were pseudomonas aeruginosa, 42 (13.4%) were pseudomonas fluorescens, 13 (4.2%) were pseudomonas mallei, 10 (3.1%) were pseudomonas putida and 4 (1.3%) were pseudomonas ...	2009	19947183
identification and characterization of a novel antibacterial peptide, avian beta-defensin 2 from ducks.	in this study, a novel avian beta-defensin (avbd) was isolated from duck pancreas. the complete nucleotide sequence of the gene contained an 195 bp open reading frame encoding 64 amino acids. homology, characterization and comparison of the gene with avbd from other avian species confirmed that it was duck avbd2. the mrna expression of the gene was analyzed in 17 tissues from 21-day-old ducks. avbd2 was highly expressed in the trachea, crop, heart, bone marrow, and pancreas; moderately expressed ...	2009	19851734
[a case of cat-scratch-induced pasteurella multocida infection presenting with disseminated intravascular coagulation and acute renal failure].	domestic animals are the main reservoirs of pasteurella species for human zoonosis due to bites and scratches. pasterurella multocida may cause serious soft-tissue infection and, less commonly, sepsis or septic shock, particularly in insufficient initial therapy and an immunocompromised host. we report a case of cat-scratch-induced p. multocida infection, presenting with disseminated intravascular coagulation and acute renal failure. a febrile 83-year-old woman with consciousness disturbance and ...	2009	19860260
single-chain urokinase in empyema induced by pasturella multocida.	intrapleural fibrin deposition and subsequent fibrosis characterize evolving empyema and contribute to the morbidity associated with this condition. single-chain urokinase (scupa) is proenzyme form of the urokinase plasminogen activator, which has recently been shown to effectively clear intrapleural loculation in tetracycline-induced pleurodesis in rabbits. the authors therefore hypothesized that scupa could likewise improve intrapleural injury associated with empyema. the authors used a rabbit ...	2009	19895321
phenotypic and genetic characterization of pasteurella multocida and related isolates from rabbits in switzerland.	several bacteria belonging to the family pasteurellaceae are potential pathogens in rabbits. in particular, pasteurella multocida is considered to be important, and outbreaks caused by this species result in considerable economic losses in rabbitries. however, pasteurellaceae spp. isolated from rabbits are poorly characterized, and thus, proper identification of p. multocida isolates from these animals is problematic and often unsatisfactory, thereby hampering epidemiological investigations. the ...	2009	19901279
an ecologic study comparing distribution of pasteurella trehalosi and mannheimia haemolytica between sierra nevada bighorn sheep, white mountain bighorn sheep, and domestic sheep.	the prevalence and phenotypic variability of pasteurella and mannheimia isolates from sierra nevada bighorn sheep (ovis canadensis sierrae), white mountain bighorn sheep (ovis canadensis nelsoni), and domestic sheep (ovis aries) from california, usa, were compared. the white mountain bighorn sheep population had a recent history of pneumonia-associated mortality, whereas the sierra nevada bighorn sheep population had no recent history of pneumonia-associated mortality. the domestic sheep flocks ...	2009	19901369
an outbreak of avian cholera in wild waterfowl in ordos wetland, inner mongolia, china.	eleven species of wild waterfowl (anseriformes and charadriiformes) were found dead in the hongjian nur lake in the ordos wetland, inner mongolia of northern china, in 2007. pasteurella multocida was isolated from tissue samples of dead and sick birds and identified as p. multocida subsp. multocida, serotype a1, using serologic and molecular techniques. eight bird species in this outbreak had never been previously reported with p. m. multocida infection. this was also the first report of fowl ch ...	2009	19901396
a survey of veterinary hospitals in nigeria for the presence of some bacterial organisms of nosocomial and zoonotic potential.	this study was undertaken to determine the type and estimate the prevalence of bacterial organisms on contact surfaces of five close-to-patient facilities in three veterinary health care settings within the sokoto metropolis of north-western nigeria. a total of 30 samples (10 from each setting) were collected and analysed using culture, microscopy and biochemical testing. bacterial species isolated from samples in this study included the following: bacillus sp. (27.3%), staphylococcus aureus (15 ...	2009	20391375
bovine respiratory disease research (1983-2009).	bovine respiratory disease (brd) research has provided significant understanding of the disease over the past 26 years. modern research tools that have been used include monoclonal antibodies, genomics, polymerase chain reaction, immunohistochemistry (ihc), dna vaccines and viral vectors coding for immunogens. emerging/reemerging viruses and new antigenic strains of viruses and bacteria have been identified. methods of detection and the role for cattle persistently infected bovine viral diarrhea ...	2009	20003649
host response to bovine respiratory pathogens.	bovine respiratory disease (brd) involves complex interactions amongst viral and bacterial pathogens that can lead to intense pulmonary inflammation (fibrinous pleuropneumonia). viral infection greatly increases the susceptibility of cattle to secondary infection of the lung with bacterial pathogens like mannheimia haemolytica and histophilus somni. the underlying reason for this viral/bacterial synergism, and the manner in which cattle respond to the virulence strategies of the bacterial pathog ...	2009	20003650
update on bacterial pathogenesis in brd.	mannheimia haemolytica, pasteurella multocida, histophilus somni, mycoplasma bovis and arcanobacterium pyogenes are all frequently implicated in bovine respiratory disease (brd). m. haemolytica is considered the most important of the group. these bacteria are commensals in the nasopharynx and establish infection in the lungs of cattle that are subjected to a variety of stresses. factors that permit adherence to and proliferation in the lungs and factors that cause tissue destruction and inflamma ...	2009	20003651
[cellulitis after a cat bite].	animal bite wounds are common. domestic companion animals inflict the majority of these wounds. the most important percentage of contagions are due to cat bites, and often by pasteurella species. we present two cases of pasteurella multocida infection after a cat bite.thus, in this article we review the most relevant clinical features related with this aetiological agent and some aspects about antimicrobial susceptibility.	2009	20082044
pasteurella multocida septicaemia in a patient on haemodialysis.	this case report details the presentation of a 76-year-old man on haemodialysis who rapidly developed overwhelming sepsis over a 24 h period with isolation of the pathogen pasteurella multocida in blood cultures. despite treatment with antibiotics shown to have good activity against this pathogen, the patient died. during further questioning, it transpired that the patient had allowed his dog to lick him, including the neck haemodialysis cannula, though no direct trauma in the form of bites or s ...	2009	21687004
expression and immunoprotective property of a 39-kda plpb protein of pasteurella multocida.	pasteurella multocida is a gram-negative bacterium known to infect a wide range of domestic and wild animals. the increasing incidence of p. multocida isolated from cases of fowl cholera and hemorrhagic septicemia has led to a renewed interest in this pathogen as well as in the development of vaccines. in this study, pcr primers were designed to amplify the fragment of plpb gene from p. multocida fc-pakchong (a:1). the purified pcr product of plpb gene consisting of 831 base pairs was inserted i ...	2009	19959899
immunity of cattle following vaccination with a mannheimia haemolytica chimeric plpe-lkt (sac89) protein.	we developed several chimeric plpe-leukotoxin (lkt) constructs containing the major epitope of mannheimia haemolytica outer membrane lipoprotein plpe (epitope r2) and the neutralizing epitope of m. haemolytica lkt (nlkt) [ayalew et al. mannheimia haemolytica chimeric protein vaccine composed of the major surface-exposed epitope of outer membrane lipoprotein plpe and the neutralizing epitope of leukotoxin. vaccine 2008;26(38):4955-61]. vaccination of mice with these plpe-lkt chimeric proteins sti ...	2009	18834912
[pasteurella multocida meningitis in a 93-year-old woman following a cat bite].	pasteurella multocida is commensal of the normal nasopharynx or gastrointestinal flora in many domestic and wild animals. cat and dog bites usually cause soft tissue infections. p. multocida is an unusual causative agent of meningitis which tends to affect neonates or aged patients. the authors report a case (a 93-year-old woman) of p. multocida meningitis induced by a cat bite with dramatic sequels leading to complete patient disability.	2009	19013736
contemporary prevalence of infectious agents in laboratory mice and rats.	periodic health screening of rodents used in research is necessary due to the consequences of unwanted infections. one determinant of the risk of infection for any given agent is its prevalence; other factors being equal, a prevalent agent is more likely than a rare one to be introduced to a research facility and result in infection. as an indicator of contemporary prevalence in laboratory populations of rats and mice, the rate of positive results in the samples received at a major commercial ro ...	2009	19015179
bacterial taxa associated with the hematophagous mite dermanyssus gallinae detected by 16s rrna pcr amplification and ttge fingerprinting.	dermanyssus gallinae (arthropoda, mesostigmata) is suspected to be involved in the transmission of a wide variety of pathogens, but nothing is known about its associated non-pathogenic bacterial community. to address this question, we examined the composition of bacterial communities in d. gallinae collected from standard poultry farms in brittany, france. genetic fingerprints of bacterial communities were generated by temporal temperature gradient gel electrophoresis (ttge) separation of indivi ...	2009	19027065
histophilus somni biofilm formation in cardiopulmonary tissue of the bovine host following respiratory challenge.	biofilms form in a variety of host sites following infection with many bacterial species. however, the study of biofilms in a host is hindered due to the lack of protocols for the proper experimental investigation of biofilms in vivo. histophilus somni is an agent of respiratory and systemic diseases in bovines, and readily forms biofilms in vitro. in the present study the capability of h. somni to form biofilms in cardiopulmonary tissue following experimental respiratory infection in the bovine ...	2009	19095078
quantification and characterization of enzymatically produced hyaluronan with fluorophore-assisted carbohydrate electrophoresis.	hyaluronan (ha) is a polysaccharide with high-potential medical applications, depending on the chain length and the chain length distribution. special interest goes to homogeneous ha oligosaccharides, which can be enzymatically produced using pasteurella multocida hyaluronan synthase (pmhas). we have developed a sensitive, simple, and fast method, based on fluorophore-assisted carbohydrate electrophoresis (face), for characterization and quantification of polymerization products. a chromatograph ...	2009	18948072
bacteremic community-acquired pneumonia due to pasteurella multocida.	an 87-year-old man was admitted to hospital because of fever, productive cough, dyspnea and altered consciousness. his medical history was significant for chronic obstructive pulmonary disease. he owned several pets. physical examination and a chest radiograph revealed right upper lobe pneumonia. blood cultures taken on admission yielded pasteurella multocida and antimicrobial susceptibility testing showed susceptibility to beta-lactams. the fever subsided four days after treatment with intraven ...	2009	18845465
bronchoalveolar lavage cytology from captive badgers.	bronchoalveolar lavage (bal) fluid is evaluated for the diagnosis and study of lung disease and airway inflammation. cytologic profiles for bal fluid have not been reported for badgers and may be useful in understanding the pathogenesis of pulmonary diseases such as mycobacterium bovis.	2009	19351340
identification and characterization of hemolysin-like proteins similar to rtx toxin in pasteurella pneumotropica.	pasteurella pneumotropica is an opportunistic pathogen that causes lethal pneumonia in immunodeficient rodents. the virulence factors of this bacterium remain unknown. in this study, we identified the genes encoding two rtx toxins, designated as pnxi and pnxii, from the genomic dna of p. pneumotropica atcc 35149 and characterized with respect to hemolysis. the pnxi operon was organized according to the manner in which the genes encoded the structural rtx toxin (pnxia), the type i secretion syste ...	2009	19363112
sialic acid uptake is necessary for virulence of pasteurella multocida in turkeys.	many pathogenic bacteria employ systems to incorporate sialic acid into their membranes as a means of protection against host defense mechanisms. in pasteurella multocida, an opportunistic pathogen which causes diseases of economic importance in a wide range of animal species, sialic acid uptake plays a role in a mouse model of systemic pasteurellosis. to further investigate the importance of sialic acid uptake in pathogenesis, sialic acid uptake mutants of an avian strain of p. multocida p-1059 ...	2009	19366625
pasteurella multocida infection in a cirrhotic patient: case report, microbiological aspects and a review of literature.	pasteurellosis is a zoonosis often caused by cat or dog bites or scratches, or by direct exposure to their secretions. pasteurella multocida is the main pathogen involved in infections through domestic animal bites; generally a local infection characterized by its particular virulence with consequent rapid onset. serious infection has also been reported in persons affected by comorbidity without domestic animal bite injuries. here we report the case of a woman with lower limb exudating vesicular ...	2009	19366651
variation in pasteurella (bibersteinia) and mannheimia spp. following transport and antibiotic treatment in free-ranging and captive rocky mountain bighorn sheep (ovis canadensis canadensis).	morbidity and mortality associated with respiratory disease following capture and translocation of bighorn sheep (ovis canadensis canadensis) is a significant concern, particularly when establishing new or augmenting existing bighorn populations. administration of prophylactic antibiotics at the time of capture is often done to minimize the risk of respiratory disease, but the efficacy of this practice is unknown. the effects of oxytetracycline and florfenicol on the pasteurella (bibersteinia) a ...	2009	19368250
pasteurella multocida toxin activation of heterotrimeric g proteins by deamidation.	pasteurella multocida toxin is a major virulence factor of pasteurella multocida, which causes pasteurellosis in men and animals and atrophic rhinitis in rabbits and pigs. the approximately 145 kda protein toxin stimulates various signal transduction pathways by activating heterotrimeric g proteins of the galpha(q), galpha(i), and galpha(12/13) families by using an as yet unknown mechanism. here, we show that pasteurella multocida toxin deamidates glutamine-205 of galpha(i2) to glutamic acid. th ...	2009	19369209
transmission of lungworms (muellerius capillaris) from domestic goats to bighorn sheep on common pasture.	four domestic goats (capra hircus) that were passing first-stage dorsal-spined larvae of muellerius capillaris were copastured on a 0.82-ha pasture for 11 mo from may 2003 to april 2004 with seven rocky mountain bighorn sheep (ovis canadensis) that were not passing dorsal-spined larvae. during the 11-mo experiment, two bighorn sheep died from pneumonia caused by mannheimia (pasteurella) haemolytica biotype a, serotype 2. the remaining five bighorn sheep and the four domestic goats remained healt ...	2009	19395736
[pasteurella multocida meningitis: report of 2 cases].		2009	19403203
rapidly progressive and lethal septicemia due to infection with pasteurella multocida in an infant.	we report a case of rapidly progressive pneumonia and meningoencephalitis in a 2-month-old breastfed infant. culture of cerebrospinal fluid was positive for pasteurella multocida. no bite or scratch injury as site of entrance could be detected clinically or at autopsy, therefore aerogenic transmission in droplets from a household pet must be considered. infections with p. multocida have been frequently reported in infants and elderly people; however, rapidly progressive and lethal infections in ...	2009	19412752
rapid virulence typing of pasteurella multocida by multiplex pcr.	the gram-negative bacterium pasteurella multocida constitutes a heterogeneous species associated with wide range of disease in many animals. isolates are classified into five groups based on capsular antigen (capa, b, d, e and f). recently, a new valuable pcr-based method was introduced to determine the epidemiological correlation between p. multocida infection and existence of virulence genes including tbpa, pfha, toxa and hgbb. however, this method is tedious and laborious. thus, in the curren ...	2009	19414187
efficacy of a novel pasteurella multocida vaccine against progressive atrophic rhinitis of swine.	the efficacy of a novel vaccine composed of three short recombinant subunit pasteurella multocida toxin (pmt) proteins in combination with a bi-valent p. multocida whole-cell bacterin (rspmt-pm) was evaluated in field studies for prevention and control of progressive atrophic rhinitis (par) of swine at 15 conventional farrow-to-finish farms. experimental piglets that were immunized twice with the rspmt-pm vaccine developed detectable titers of neutralizing antibodies (greater than 1:8) that prev ...	2009	19428902
antimicrobial susceptibility and resistance of chicken escherichia coli, salmonella spp., and pasteurella multocida isolates.	escherichia coli, salmonella species, and pasteurella multocida are the major bacterial pathogens isolated from poultry. difference in susceptibility to antibiotics by microorganisms has become a major factor in drug choice and success of treatment. great concerns have been raised regarding emerging antimicrobial resistance among bacteria that may result in unpredictable antimicrobial susceptibility and failure of therapy. the primary objective of the present study was to determine the levels of ...	2009	19432009
epidural abscess caused by haemophilus aphrophilus misidentified as pasteurella species.	haemophilus aphrophilus is one of the normal oropharyngeal flora and rarely implicated as a pathogen of spinal infection. a case of h. aphrophilus bacteremia complicated with epidural abscess, psoas muscle abscess, and spondylodiscitis is described in this report. the pathogen was mis-identified as pasteurella spp. at the very start, and was confirmed by the molecular method. he was successfully treated with adequate antibiotics and surgery. the clinical features of sixteen previously reported c ...	2009	19443984
a retrospective six-year national survey of p. multocida infections in israel.	pasteurella multocida is the commonest organism infecting pet bites. anecdotal reports tend to overemphasize dramatic outcomes. we aimed to study a large database of p. multocida infections. this retrospective survey of p. multocida infections in israeli hospitals refers to the y 2000-2005. clinical microbiologists were contacted by email and asked to perform a back-search of their hospital's records for isolates of p. multocida. the charts of patients growing p. multocida were abstracted into a ...	2009	19452350
molecular diversity of porcine and human isolates of pasteurella multocida.	to examine the variability among pasteurella multocida strains isolated from pigs (nasal, tonsil and lung specimens) and humans in france.	2009	19457034
[septic arthritis and osteomyelitis caused by pasteurella multocida: a new case].		2009	19457335
development and evaluation of an antigen-capture elisa for detection of the ul24 antigen of the duck enteritis virus, based on a polyclonal antibody against the ul24 expression protein.	an antigen-capture enzyme-linked immunosorbent assay (ac-elisa) method was developed for the efficient detection of the ul24 antigen of the duck enteritis virus (dev) using polyclonal antibodies. ducks and rabbits were immunized, respectively, with expressed ul24 recombinant protein. the igg antibodies against ul24 from ducks and rabbits were purified and used as the capture antibodies. the specificity of the optimized ac-elisa was evaluated by use of dev, duck hepatitis virus (dhv), duck hepati ...	2009	19467266
isolation, antimicrobial resistance, and virulence genes of pasteurella multocida strains from swine in china.	a total of 233 isolates of pasteurella multocida were obtained from 2,912 cases of clinical respiratory disease in pigs in china, giving an isolation rate of 8.0%. serogroup a p. multocida isolates were isolated from 92 cases (39.5%), and serogroup d isolates were isolated from 128 cases (54.9%); 12 isolates (5.2%) were untypeable. p. multocida was the fourth most frequent pathogenic bacterium recovered from the respiratory tract, after streptococcus suis, haemophilus parasuis, and escherichia c ...	2009	19158260
identification of novel glycosyltransferases required for assembly of the pasteurella multocida a:1 lipopolysaccharide and their involvement in virulence.	we previously determined the structure of the pasteurella multocida heddleston type 1 lipopolysaccharide (lps) molecule and characterized some of the transferases essential for lps biosynthesis. we also showed that p. multocida strains expressing truncated lps display reduced virulence. here, we have identified all of the remaining glycosyltransferases required for synthesis of the oligosaccharide extension of the p. multocida heddleston type 1 lps, including a novel alpha-1,6 glucosyltransferas ...	2009	19168738
host-pathogen interactions of actinobacillus pleuropneumoniae with porcine lung and tracheal epithelial cells.	host-pathogen interactions are of great importance in understanding the pathogenesis of infectious microorganisms. we developed in vitro models to study the host-pathogen interactions of porcine respiratory tract pathogens using two immortalized epithelial cell lines, namely, the newborn pig trachea (nptr) and st. jude porcine lung (sjpl) cell lines. we first studied the interactions of actinobacillus pleuropneumoniae, an important swine pathogen, using these models. under conditions where cytot ...	2009	19139196
respiratory disease in calves: microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response.	trans-tracheal aspirations from 56 apparently healthy calves and 34 calves with clinical signs of pneumonia were collected in six different herds during september and november 2002. the 90 samples were cultivated and investigated by pcr tests targeting the species histophilus somni, mannheimia haemolytica, pasteurella multocida, mycoplasma bovis, mycoplasma dispar, and mycoplasma bovirhinis. a pcr test amplifying the lktc-artj intergenic region was evaluated and shown to be specific for the two ...	2009	19186010
pathological changes in the respiratory tract of goats infected by pasteurella multocida b:2.	clinical and pathological changes are described in groups of five goats pretreated with dexamethasone and then infected with a large dose of pasteurella multocida b:2 (the cause of haemorrhagic septicaemia) by the intratracheal, subcutaneous or intranasal route (groups a, b and c, respectively). in group a, two goats died (on day 1 and 4 post-inoculation); in group b three died (days 2, 5 and 14); and in group c one died (day 20). the infecting organism was recovered from the four goats that die ...	2009	19110260
first case of pasteurella multocida endocarditis of the tricuspid valve: a favorable outcome following medical treatment.	we describe a rare case of tricuspid valve endocarditis caused by pasteurella multocida in an elderly woman with no previous history of valvular heart disease. risk factors included contact with animals and old age. the patient was treated successfully with five days of intravenous ampicillin followed by four weeks of oral ciprofloxacin.	2009	19121968
pasteurella multocida toxin activates gbetagamma dimers of heterotrimeric g proteins.	the mitogenic pasteurella multocida toxin (pmt) is a major virulence factor of p. multocida, which causes pasteurellosis in man and animals. the toxin activates the small gtpase rhoa, the map kinase erk and stat proteins via the stimulation of members of two g protein families, g(q) and g(12/13). pmt action also results in an increase in inositol phosphates, which is due to the stimulation of plcbeta via galpha(q). recent studies indicate that pmt additionally activates galpha(i) to inhibit aden ...	2009	19135527
complete chemoenzymatic synthesis of the forssman antigen using novel glycosyltransferases identified in campylobacter jejuni and pasteurella multocida.	we have identified an alpha1,4-galactosyltransferase (cgtd) and a beta1,3-n-acetylgalactosaminyltransferase (cgte) in the lipooligosaccharide (los) locus of campylobacter jejuni lio87. strains that carry these genes may have the capability of synthesizing mimics of the p blood group antigens of the globoseries glycolipids. we have also identified an alpha1,3-n-acetylgalactosaminyltransferase (pm1138) from pasteurella multocida pm70, which is involved in the synthesis of an los-bound forssman ant ...	2009	18955372
immunohistochemical detection of mannheimia (pasteurella) haemolytica antigens in goats with natural pneumonia.	pneumonia is a leading cause of loss to ruminants throughout the world. mannheimia (pasteurella) haemolytica is one of the most important etiological agent of pneumonia in cattle, sheep, and goats. this study was carried out to determine the incidence of m.haemolytica antigens using immunohistochemistry labelling of formalin-fixed, paraffin-embedded tissues in pneumonic lungs of goats slaughtered at abattoir, and then to compare these immunohistochemistry results with the results of bacterial is ...	2009	18972219
promotion of immunity of mice to pasteurella multocida and hog cholera vaccine by pig interleukin-6 gene and cpg motifs.	a novel oligodeoxynuleotides containing 11 cpg motifs was synthesized and inserted into the vr1020 plasmid containing pig interleukin-6 (il-6) gene (vpil6) to construct recombinant plasmid, vpil6c. the chitosan nanoparticles (cnp) were prepared by ionic cross linkage to entrap the vpil6c (vpil6c-cnp), vpil6 (vpil6-cnp) and cpg (cpg-cnp). 42-day old female mice were divided into four groups and intramuscularly injected respectively with 6 pmol vpil6c-cnp, vpil6-cnp, cpg-cnp and vr1020-cnp along w ...	2009	18279956
usage of antimicrobials and occurrence of antimicrobial resistance among bacteria from mink.	the usage of antimicrobials for treatment of mink and the occurrence of antimicrobial resistance among the most important bacterial pathogens in mink was investigated. the aim of the study was to provide data, which may serve as a basis for the formulation of recommendations for prudent use of antimicrobials for mink. a total of 164 haemolytic staphylococci, 49 haemolytic streptococci, 39 pseudomonas aeruginosa, 13 pasteurella multocida, and 1093 escherichia coli isolates from danish mink were i ...	2009	18620819
identification of pasteurella multocida isolates of ruminant origin using polymerase chain reaction and their antibiogram study.	a total of 100 isolates of pasteurella multocida from various ruminant species (cattle, buffalo and sheep) belonging to different parts of country were identified using pasteurella multocida-pcr (pm-pcr) and capsular pcr assays. pm-pcr revealed an amplicon of approximately 460 bp in all the isolates tested. as regards capsular pcr, 36 of 38 cattle isolates and 30 of 34 buffalo isolates were found to belong to capsular serogroup b whereas rest of the cattle and buffalo isolates belonged to serogr ...	2009	18759064
development and application of a reverse transcriptase polymerase chain reaction to detect chinese isolates of duck hepatitis virus type 1.	we developed a reverse transcriptase polymerase chain reaction (rt-pcr) method for the detection of duck hepatitis virus type 1 (dhv-1) in the tissues of infected and clinically affected ducks and in chick and duck embryos. we found the assay to be effective in detecting the virus in china, where it is being used in studies on the epidemiology of the disease. we applied this simple and rapid diagnostic method to the detection of dhv isolates grown in chick and duck embryos and in tissues obtaine ...	2009	18706944
meningitis and subgaleal, subdural, epidural empyema due to pasteurella multocida.	pasteurella is a gram-negative coccobacillus found in 70-90% of oral cavities of cats, and as well, is isolated from the digestive systems of dogs, rats, rabbits, monkeys, and other animals. pasteurella multocida has been known to cause infections in humans, the most familiar being soft tissue infection after animal bites. however, this organism may affect a variety of systems, causing serious disease. pasteurella multocida can cause septic arthritis, osteomyelitis, pneumonia, endocarditis, meni ...	2010	18486411
mannan-binding lectin (mbl) in two chicken breeds and the correlation with experimental pasteurella multocida infection.	the present study is the first demonstration of an association of the genetic serum mannan-binding lectin (mbl) concentration with bacterial infections in chickens. the genetic serum mbl concentration was determined in two chicken breeds, and the association with the specific pasteurella multocida humoral immune response during an experimental infection was examined. furthermore, we examined the association of the genetic serum mbl concentration with systemic infection. the chickens with systemi ...	2010	18922580
immunogenicity of iron-regulated outer membrane proteins of pasteurella multocida b:2 in mice model.	the present study was conducted to investigate the role of iron-regulated outer membrane proteins (iromp) of pasteurella multocida b:2 in mice as potential immunogens. outer membrane proteins extracted from p. multocida b:2 grown under normal (omp) and iron-deficient (iromp) conditions were subjected to discontinuous sds-page. nine polypeptides of mw ranging from 85.1 to 16.7 kda from omp preparations and two additional polypeptides of mw 95.4 and 89.1 kda from iromp preparations were observed w ...	2010	21250599
multiplex pcr assay for detection of actinobacillus pleuropneumoniae, pasteurella multocida and haemophilus parasuis in lungs of pigs from a slaughterhouse.	multiplex pcr has been developed for parallel identification of actinobacillus pleuropneumoniae, pasteurella multocida and haemophilus parasuis, important pathogens of swine, responsible for considerable economic losses in swine industry. multiplex pcr and bacteriological cultivation was used to analyze lung samples from slaughterhouse pigs. from a total of 219 lung samples, 164 (74.9 %) were positive for p. multocida, 45 (20.5 %) for a. pleuropneumoniae and 4 (1.83 %) for h. parasuis. bacteriol ...	2010	21253912
prevalence of pasteurella multocida and other respiratory pathogens in the nasal tract of scottish calves.	the prevalence of pasteurella multocida, a cause of bovine respiratory disease, was studied in a random sample of beef suckler and dairy farms throughout scotland, by means of a cross-sectional survey. a total of 637 calves from 68 farms from six geographical regions of scotland were sampled between february and june 2008. deep nasal swabs were taken, and samples that were culture-positive for p multocida were confirmed by pcr. prevalence of p multocida was 17 per cent (105 of 616 calves); 47 pe ...	2010	21257416
outbreak of polyarthritis in lambs attributed to pasteurella multocida.		2010	21257563
assessment of rpob and 16s rrna genes as targets for pcr-based identification of pasteurella pneumotropica.	diagnosis of pasteurella pneumotropica in laboratory animals relies on isolation of the organism, biochemical characterization, and, more recently, dna-based diagnostic methods. 16s rrna and rpob gene sequences were examined for development of a real-time pcr assay. partial sequencing of rpob (456 bp) and 16s rrna (1368 bp) of pasteurella pneumotropica isolates identified by microbiologic and biochemical assays indicated that either gene sequence can be used to distinguish p. pneumotropica from ...	2010	21262128
necrotizing fasciitis complicated by fatal multiple organ dysfunction due to pasteurella multocida infection: a case report.	necrotizing fasciitis is rarely caused by pasteurella multocida, a facultative anaerobic gram-negative coccobacillus found in the flora of the oro-gastrointestinal tract of many animals. we describe a rare case with overwhelming septicaemia resulting in multiple organ failure.	2010	21268955
cloning, expression and protective capacity of 37 kda outer membrane protein gene (omph) of pasteurella multocida serotype b:2.	the major outer membrane protein (omph) of 4 local malaysian strains of pasteurella multocida serotype b:2 were characterized in comparison to atcc strains. three major peptide bands of mw 26, 32 and 37 kda were characterized using sdspage. two of these fragments, the 32 kda and 37 kda were observed to be more reactive with a mouse polyclonal antiserum in all of the local isolates as well as the atcc strains in a western blot. however, the 32 kda fragment was found to cross react with other gram ...	2010	21399583
pasteurella multocida toxin activates various heterotrimeric g proteins by deamidation.	pasteurella multocida produces a 146-kda protein toxin (pasteurella multocida toxin, pmt), which stimulates diverse cellular signal transduction pathways by activating heterotrimeric g proteins. pmt deamidates a conserved glutamine residue of the a-subunit of heterotrimeric g proteins that is essential for gtp-hydrolysis, thereby arresting the g protein in the active state. the toxin substrates are ga(q) ga(13) and the ga(i)-family proteins. activation of these a-subunits causes stimulation of p ...	2010	22069582
antigenicity of partial fragments of recombinant pasteurella multocida toxin.	pasteurella multocida serogroup d strain, which produces p. multocida toxin (pmt), is a widespread and harmful pathogen of respiratory diseases such as pneumonia and progressive atrophic rhinitis (par) in swine. vaccination has been considered the most desirable and effective approach for controlling the diseases caused by toxigenic p. multocida. to investigate the antigenicity and immunogenicity of partial fragments of recombinant pmt, recombinant proteins of the n-terminal (pmt-a), middle (pmt ...	2010	21193834
influence of methylation on the antibacterial properties of triclosan in pasteurella multocida and pseudomonas aeruginosa variant strains.	the opportunistic bacterium pasteurella multocida is extremely susceptible to the hydrophobic biocide triclosan by virtue of its markedly permeable outer membrane, while the nosocomial pathogen pseudomonas aeruginosa is intrinsically resistant to levels far exceeding the triclosan aqueous solubility limit. widespread incorporation of triclosan in health and personal care products has resulted in its concomitant accumulation with metabolites such as methyl triclosan in environmental and biologica ...	2010	21194793
plasma and tissue pharmacokinetics of danofloxacin in healthy and in experimentally infected chickens with pasteurella multocida.		2010	21219355
pasteurella multocida bacteremia, spontaneous bacterial peritonitis and septic arthritis in a cirrhotic patient.		2010	21311647
causes of mortality in commercial organic layers in denmark.	a longitudinal study investigated the courses of mortality in commercial free-range organic layer flocks in denmark. in total, 15 organic egg-producing flocks from 11 farms were randomly selected among 80 farms registered in denmark. four farms with confined egg production on deep litter were included for comparison. flock sizes ranged from 2260 to 5940 layers. the flocks were monitored from introduction to the layer farm until slaughter. flock mortalities ranged from approximately 2% to 91%, wi ...	2010	21313846
the forgotten giants behind louis pasteur: contributions by the veterinarians toussaint and galtier.		2010	21466009
molecular heterogeneity of plpe gene in indian isolates of pasteurella multocida and expression of recombinant plpe in vaccine strain of p. multocida serotype b: 2.	outer membrane proteins of pasteurella (p.) multocida have been known to be protective immunogens. pasteurella lipoprotein e (plpe) has been reported to be an important cross reactive outer membrane protein in p. multocida. the gene encoding the plpe of p. multocida serotypes a: 3, b: 2 and d: 1 was amplified from the genomic dna. the amplified products were cloned and the nucleotide sequence was determined. sequence analysis of the recombinant clones revealed a single open reading frame of 1,01 ...	2010	20706030
microbiological and pathological examination of fatal calf pneumonia cases induced by bacterial and viral respiratory pathogens.	the infectious origin of fatal cases of calf pneumonia was studied in 48 calves from 27 different herds on postmortem examination. lung tissue samples were examined by pathological, histological, bacterial culture, virus isolation and immunohistochemical methods for the detection of viral and bacterial infections. pneumonia was diagnosed in 47/48 cases and infectious agents were found in 40/47 (85%) of those cases. the presence of multiple respiratory pathogens in 23/40 (57.5%) cases indicated t ...	2010	20713325
development of a novel lamp diagnostic method for visible detection of swine pasteurella multocida.	a set of four specific primers for six regions of kmt1 gene from a species specific region was designed for developing the loop-mediated isothermal amplification diagnostic method of swine pasteurella multocida (pm-lamp). after the pm-lamp was carried out at 63°c for 1 h, the lamp products could be visually confirmed using fluorescent dyes as detection reagent under uv-illumination. in sensitivity, the detection limit of the pm-lamp was 10 cfu/ml, and was 1 log less than that of the pcr method. ...	2010	20717843
recent insights into pasteurella multocida toxin and other g-protein-modulating bacterial toxins.	over the past few decades, our understanding of the bacterial protein toxins that modulate g proteins has advanced tremendously through extensive biochemical and structural analyses. this article provides an updated survey of the various toxins that target g proteins, ending with a focus on recent mechanistic insights in our understanding of the deamidating toxin family. the dermonecrotic toxin from pasteurella multocida (pmt) was recently added to the list of toxins that disrupt g-protein signa ...	2010	20722598
disease patterns and immune responses in the offspring to sows with high or low antibody levels to actinobacillus pleuropneumoniae serotype 2.	the serum antibody responses to actinobacillus pleuropneumoniae and the secondary invader pasteurella multocida were monitored from birth until slaughter in the offspring to sows with high or low levels of serum antibodies to a. pleuropneumoniae. serum antibody concentrations to a. pleuropneumoniae were higher from birth to the age of 9weeks in piglets delivered by high responding sows. in contrast, antibody levels to p. multocida were similar in both groups during this period. from the age of 2 ...	2010	20728191
the synthesis of paleic acid, an antimicrobial agent effective against mannheimia and pasteurella, and its structurally related derivatives.	a synthetic route to paleic acid 1, antimicrobial agent effective against mannheimia haemolytica and pasteurella multocida, has been established. the absolute configuration of the secondary hydroxyl group was controlled by a catalytic asymmetric alkylation of an aldehyde using a chiral titanium sulfonamide complex and the cis double bond was installed using a wittig reaction. this synthetic route was also applied to the preparation of structurally related analogs, which were used in structure-ac ...	2010	20728353
respiratory tract colonization by pasteurella pneumotropica in a patient with an alpha1-antitrypsin deficiency unexpectedly well identified by automated system vitek 2.		2010	20846595
immunolocalization of pulmonary intravascular macrophages, tlr4, tlr9 and il-8 in normal and pasteurella multocida-infected lungs of water buffalo (bubalus bubalis).	water buffalo are of considerable economic significance in south east asia, but these animals suffer from many bacterial respiratory diseases including haemorrhagic septicaemia caused by pasteurella multocida. bacterial respiratory diseases of animals cause lung inflammation that is characterized by the activation of toll-like receptors (tlrs) expressed on macrophages, expression of chemokines and recruitment of neutrophils and monocytes. pulmonary intravascular macrophages (pims) present in the ...	2010	20880542