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genetic and biological characteristics of avian influenza virus subtype h1n8 in environments related to live poultry markets in china.since 2008, avian influenza surveillance in poultry-related environments has been conducted annually in china. samples have been collected from environments including live poultry markets, wild bird habitats, slaughterhouses, and poultry farms. multiple subtypes of avian influenza virus have been identified based on environmental surveillance, and an h1n8 virus was isolated from the drinking water of a live poultry market.201931117981
sitting ducklings: timing of hatch, nest departure, and predation risk for dabbling duck broods.for ground-nesting waterfowl, the timing of egg hatch and duckling departure from the nest may be influenced by the risk of predation at the nest and en route to wetlands and constrained by the time required for ducklings to imprint on the hen and be physically able to leave the nest. we determined the timing of hatch, nest departure, and predation on dabbling duck broods using small video cameras placed at the nests of mallard (anas platyrhynchos; n = 26), gadwall (mareca strepera; n = 24), and ...201931110697
de novo assembly of the indian blue peacock (pavo cristatus) genome using oxford nanopore technology and illumina sequencing.the indian peafowl (pavo cristanus) is native to south asia and is the national bird of india. here we present a draft genome sequence of the male blue peacock using illumina and oxford nanopore technology (ont).201931077316
changes in morphology and mirnas expression in small intestines of shaoxing ducks in response to high temperature.during summer days the extreme heat may cause damage to the integrity of animal intestinal barrier. little information is available concerning morphological changes in the duck intestines in response to high temperature. and the molecular mechanisms underlying the pathogenesis of high temperature-induced intestinal injury remain undefined. micrornas (mirnas) are known to play key roles in post-transcriptional regulation of gene expression that influences various biological processes. the purpose ...201931049835
near-complete genome sequence of avian influenza virus strain a/mallard/balkhash/6304/2014 (h1n1) from kazakhstan.an avian influenza virus strain, a/mallard/balkhash/6304/2014 (h1n1), was isolated during a wild bird monitoring study in kazakhstan in 2014. the virus was isolated from a wild mallard duck (anas platyrhynchos) in eastern kazakhstan. here, we present the near-complete genome sequence of the virus.201931048397
molecular cloning and functional characterisation of duck (anas platyrhynchos) tumour necrosis factor receptor-associated factor 3.1. tumour necrosis factor receptor-associated factor 3 (traf3) is a key regulator of innate immunity and acquired immunity, and has a salient anti-viral role. 2. in this experiment, the duck traf3 (dutraf3) gene was cloned according to the anas platyrhynchostraf3 sequence to explore its function. the traf3 open reading frame contains 1704 bp that encode a protein of 567 amino acids, which contain a ring finger domain, two zinc finger motifs, a coiled-coil region, and a math domain. 3. reverse tr ...201931046421
dynamic expression pattern of serpina1 gene from duck (anas platyrhynchos).serpina1 is a member of serine protease inhibitors and is increasingly considered to be a regulator of innate immunity in human and animals. however, the expression and function of serpina1 gene in immune defense against viral infection remain unknown in ducks. the full-length du serpina1 cdna sequence was obtained using reverse transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). it contained 1457 nucleotide, including 47-bp 5' utr, 135-bp 3' utr, and 12 ...201931016183
continuing evolution of highly pathogenic h5n1 viruses in bangladeshi live poultry markets.since november 2008, we have conducted active avian influenza surveillance in bangladesh. clades 2.2.2, 2.3.4.2, and 2.3.2.1a of highly pathogenic avian influenza h5n1 viruses have all been identified in bangladeshi live poultry markets (lpms), although, since the end of 2014, h5n1 viruses have been exclusively from clade 2.3.2.1a. in june 2015, a new reassortant h5n1 virus (h5n1-r1) from clade 2.3.2.1a was identified, containing haemagglutinin, neuraminidase, and matrix genes of h5n1 viruses ci ...201931014196
comparing erosion and organ accumulation rates of lead and alternative lead-free ammunition fed to captive domestic ducks.lead poisoning of birds of prey from ingestion of ammunition lead has been well documented. alternative, lead-free ammunition is widely available, but the toxicokinetics of other metals in birds are poorly understood. we tested the erosion of lead, copper, zinc, iron and brass by feeding domestic pekin ducks (anas platyrhynchos forma domestica) defined numbers of small metal pellets. the accumulation of these metals was analysed in the breast muscle, brain, pancreas, liver and kidney. four weeks ...201930993577
galectin-1 induces immune response and antiviral ability in cherry valley ducks after duck plague virus infection.galectin-1, as a typical animal galactose-binding protein, it is found on the cell surface and in the extracellular matrix. cloning the full-length coding sequence of galectin-1 from the spleens of cherry valley ducks revealed that the coding sequence of duck galectin-1 (dugal-1) comprises 405 bp, encoding 134 amino acids. homologic analysis revealed its amino acid sequence is most identical to that of anas platyrhynchos (98.8%) followed by gallus gallus. quantitative real-time pcr analysis indi ...201930986433
transcriptional regulatory region and dna methylation analysis of tnni1 gene promoters in gaoyou duck skeletal muscle (anas platyrhynchos domestica).1. the slow skeletal muscle troponin i (tnni1) gene has been found to be specifically expressed in slow muscle fibres and plays an important role in muscle development. the aim of this study was to determine the active control area of duck tnni1 and identify the potential cis-regulatory elements in the promoter. 2. in this study, the tnni1 promoter was first cloned by genome walking and the sequences were analysed using bioinformatics software. firefly luciferase reporter gene vectors, driven by ...201930968708
amyloid a amyloidosis secondary to avian tuberculosis in naturally infected domestic pekin ducks (anas platyrhynchos domestica).to investigate the correlation between avian tuberculosis and duck amyloidosis, the liver, lung, spleen, kidney, duodenum and pectoralis muscle of ducks naturally infected with mycobacterium avium subsp. avium were used to detect amyloidosis by congo red staining and potassium permanganate-congo red staining. the expression level of il-1β, il-6, il-10, tnf-α and saa2 were detected by quantitative real-time rt-pcr (qrt-pcr). the results showed that the liver, lung, spleen, kidney, duodenum and pe ...201930961809
comparison of whole embryonic development in the duck (anas platyrhynchos) and goose (anser cygnoides) with the chicken (gallus gallus).embryos from aquatic birds are the primary models for the study of flipper development. while some staging of early embryogenesis in duck have been studied, characterization of the stages of the entire embryonic development period in water birds has not been described. this study aimed to establish a comparison of complete morphological development staging for ducks (anas platyrhynchos) and geese (anser cygnoides) with the embryonic staging system by hamburger and hamilton (hh) for the chicken ( ...201930941418
molecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three anseriformes species.the highly repetitive dna sequence of centromeric heterochromatin is an effective molecular cytogenetic marker for investigating genomic compartmentalization between macrochromosomes and microchromosomes in birds. we isolated four repetitive sequence families of centromeric heterochromatin from three anseriformes species, viz., domestic duck (anas platyrhynchos, apl), bean goose (anser fabalis, afa), and whooper swan (cygnus cygnus, ccy), and characterized the sequences by molecular cytogenetic ...201930913221
isolation and characterization of a novel h7n8 avian influenza virus from domestic ducks in central china in 2017.in 2017, an h7n8 avian influenza virus (aiv) was isolated from a domestic duck from a farm in central china. sequences analysis showed that this strain received its genes from h7, h1, h2, h3, h5, and h6 aivs of domestic poultry and wild birds in asia. it exhibited low pathogenicity in chickens and mild pathogenicity in mice. these results suggest the importance of continued surveillance of the h7n8 virus to better understand the ecology and evolution of the aivs in poultry and wild birds and the ...201930895439
mallard resource selection trade-offs in a heterogeneous environment during autumn and winter.animals select resources to maximize fitness but associated costs and benefits are spatially and temporally variable. differences in wetland management influence resource availability for ducks and mortality risk from duck hunting. the local distribution of the mallard (anas platyrhynchos) is affected by this resource heterogeneity and variable risk from hunting. regional conservation strategies primarily focus on how waterfowl distributions are affected by food resources during the nonbreeding ...201930847073
varied effects of dietary carotenoid supplementation on oxidative damage in tissues of two waterfowl species.carotenoids are regarded as a cornerstone of avian vitality and coloration. currently, the antioxidant potential of dietary carotenoids is debated for birds. although some studies support a protective role, others report either no effect or pro-oxidant effects. however, the majority of research on this topic has not analyzed the oxidative status of a series of tissues in animals nor considered a range of carotenoid dosages. we investigated the effects of three levels of carotenoid supplementatio ...201930794961
danish pheasant and mallard hunters comply with the lead shot ban.denmark implemented a total ban on the use of lead shot for hunting and clay target shooting in 1996. compliance was not studied systematically until recently. however, sporadic police checks and individual studies indicated that compliance was far from complete in the early years after regulation. to assess current levels of compliance with danish regulations, we purchased 730 carcasses of pheasants (phasianus colchicus) and mallards (anas platyrhynchos) and a further 690 mallard gizzards were ...201930771205
different pathogenicity of two strains of clade 2.3.4.4c h5n6 highly pathogenic avian influenza viruses bearing different pa and ns gene in domestic ducks.h5nx clade 2.3.4.4 highly pathogenic avian influenza viruses (hpaivs) have been disseminated to wide geographic regions since 2014. in 2016, five distinct genotypes (c-1 to c-5) of clade 2.3.4.4c h5n6 hpaivs were detected in south korea. in this study, we evaluated the pathogenicity, susceptibility to infection, and transmissibility of the two strains representing the c-1 and c-4 genotypes of the h5n6 viruses, which have different pa and ns gene, in domestic ducks. although the susceptibility to ...201930753976
comparative pathogenicity of h5n6 subtype highly pathogenic avian influenza viruses in chicken, pekin duck and muscovy duck.in japan during the 2016-2017 winter season, clade 2.3.4.4 highly pathogenic avian influenza viruses (hpaivs) of the h5n6 subtype caused 12 outbreaks in chicken and muscovy duck farms. these viruses have been circulating in vietnam and china since 2014. in this study, we evaluated the susceptibility of chicken, pekin duck (anas platyrhynchos domesticus) and muscovy duck (cairina moschata) to h5n6 hpaivs that originated in japan, vietnam and china. the h5n6 hpaivs examined in this study were high ...201930720248
effects of salmonella enterica serovar enteritidis infection on egg production and the immune response of the laying duck anas platyrhynchos.persistent colonization of the avian reproductive tract by salmonella enterica serovar enteritidis (se) negatively affects egg production and contaminates the egg. the immune function of the ovary and oviduct is essential for protection from infection and for the production of wholesome eggs. however, the immune response of laying ducks during se infection is not well-understood. in this study, ducks (anas platyrhynchos) were infected with se and were systematically monitored for fecal shedding ...201930701142
individual and flock immunity responses of naïve ducks on smallholder farms after vaccination with h5n1 avian influenza vaccine: a study in a province of the mekong delta, vietnam.in vietnam, vaccination has played a crucial role in the national strategy for the prevention and control of h5 highly pathogenic avian influenza (hpai). this study aimed to evaluate antibody responses of immunologically naïve domestic ducks to h5n1 avian influenza vaccine currently used in the national mass vaccination program of vietnam. blood samples of 166 ducks reared on smallholder farms were individually collected at three sampling time points, namely, right before vaccination, 21 days af ...201930671307
genetic characterization and epidemiological implications of campylobacter isolates from wild birds in south korea.in this study, we genotyped campylobacter isolates from wild birds by multilocus sequence typing (mlst) and analysed their virulence genes by pcr with the aim to gain a deeper understanding of the epidemiology of campylobacter infection. amongst 60 campylobacter isolates from 12 wild bird species, we identified 32 sequence types (sts; 29 sts from campylobacter jejuni and 3 sts from campylobacter coli). clonal complex 45 (cc-45), was the most common cc (n = 17 isolates), followed by cc-692 (n = 1 ...201929959817
influenza's newest trick.influenza a viruses are important pathogens for humans and for many birds and mammals. hemagglutinin and neuraminidase are the major surface proteins of this enveloped rna virus. hemagglutinin requires proteolytic cleavage for activation, but because the viral genome does not encode its own protease, an exogenous serine protease must be provided by host cells. a novel, neuraminidase-dependent mechanism for hemagglutinin activation was described, in which a thrombin-like protease allows an influe ...201931822589
molecular cloning, characterisation, and expression analysis of adipocyte fatty acid binding protein gene in xupu goose (anser cygnoides domesticus).1. adipocyte fatty acid binding protein (a-fabp) plays a key role in fatty acid uptake and intracellular transport. the objective of the present study was to identify and characterise the a-fabp gene in xupu goose.2. the full-length cdna of goose a-fabp gene was cloned from the liver tissue using reverse transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). the distribution of the goose a-fabp in different tissues was determined by quantitative real-time p ...201931509442
are microneutralization and hemagglutination inhibition assays comparable? serological results from influenza experimentally infected mallard ducks.the hemagglutination inhibition (hi) assay is commonly used to assess the humoral immune response against influenza a viruses (iav). however, the microneutralization (mn) assay has been reported to have higher sensitivity when testing sera from humans and other species. our objective was to determine the agreement between mn and hi assays and compare the proportion of positive samples detected by both methods in sera of mallards primary infected with the a/mallard/mn/sg-000169/ 2007 (h3n8) virus ...201931131570
endoparasites of domesticated animals that originated in the neo-tropics (new world tropics).this review serves to summarize parasites found in domesticated animals which were found in the neo-tropics. indigenous domesticated neo-tropical animals include south american camelids, (lama gunacoa, lama glama, lama pacos, vicuna vicuna), guinea pigs (cavia porcellus), chinchillas (chinchilla lanigera), turkeys (meleagris gallopavo) and ducks (cairina moschata, anas platyrhynchos, dendrocyga autumnalis). these animals were chosen due to their origin of existence (neo-tropics) and over time th ...201930845667
identification and analysis of long non-coding rnas in response to h5n1 influenza viruses in duck (anas platyrhynchos).long non-coding rnas (lncrnas) are important component of mammalian genomes, where their numbers are even larger than that of protein-coding genes. for example, human (homo sapiens) (96,308 vs. 20,376) and mouse (mus musculus) (87,774 vs. 22,630) have more lncrna genes than protein-coding genes in the noncodev5 database. recently, mammalian lncrnas were reported to play critical roles in immune response to influenza a virus infections. such observation inspired us to identify lncrnas related to ...201930634898
sarcocystis rileyi emerging in hungary: is rice breast disease underreported in the region?reports of sarcocystis rileyi-like protozoa ('rice breast disease') from anseriform birds had been rare in europe until the last two decades, when s. rileyi was identified in northern europe and the uk. however, despite the economic losses resulting from s. rileyi infection, no recent accounts are available on its presence (which can be suspected) in most parts of central, western, southern and eastern europe. between 2014 and 2019, twelve mallards (anas platyrhynchos) were observed to have rice ...201931549541
molecular and pharmacological characterization of poultry (gallus gallus, anas platyrhynchos, anser cygnoides domesticus) and pig (sus scrofa domestica) melanocortin-5 receptors and their mutants.the melanocortin-5 receptor (mc5r) is a member of the g protein-coupled receptor superfamily that plays a critical role in lipid production, skeletal muscle fatty acid oxidation, and adipocyte lipolysis. although multiple functions and important value of mc5r in human beings have been fully demonstrated, however, the potential molecular cloning, pharmacological characteristics and key amino acids in poultry and pig were still not fully understood. herein, we successfully cloned mc5r genes from c ...201931356812
relationship between residual feed intake and production traits in a population of f2 ducks.improving feed efficiency is important for decreasing feed cost in poultry production, because feed account for approximately 70% of the total production costs. the selection of feed efficiency may affect other important economic traits. therefore, the objectives of this present study was to evaluate the relationships of the residual feed intake (rfi) with live body weight, carcass weight, carcass composition, and size of small intestines in a population of f2 pekin ducks. nine-hundred and eight ...201932055193
effect of bacterial endotoxin lipopolysaccharide treatment on duck leydig cells.this study aimed to investigate the effects of bacterial endotoxin lipopolysaccharide (lps) on hormone production and gene expression in duck leydig cells and its underlying mechanisms. leydig cells were collected from 200-day-old mallard ducks and divided into five treatment groups (0, 50, 100, 200, and 400 ng/ml lps). after treatment with lps for 6, 12, 24, and 48 h, testosterone, activin, and inhibin levels in the cell supernatants were determined using enzyme-linked immunosorbent assay (elis ...201932368266
molecular detection of gamma coronaviruses in bird parks of iran.gamma coronaviruses (gcovs) are distributed worldwide, affecting a wide range of bird species, the beluga whale, and bottlenose dolphins. because of the limited proofreading capability in the viral encoded polymerase, they emerge genetically diverse. there has been no molecular surveillance data to describe the epidemiology of gcovs in avian species. the present study was conducted to detect gcovs in tehran birds’ parks, 2015. cloacal swabs (267 samples) from eight different bird speci ...201931939251
gross, histochemical and electron microscopical characterization of the pecten oculi of baladi ducks (anas boschas domesticus).as pecten oculi had great functional significances for ornithology, pecten oculi of baladi duck was well-deserving of intensive morphological study. so, the aim of this study was to throw light on some anatomical and histological formation of the pecten oculi of baladi ducks as well as use of scanning electron microscopy.201931819872
botulism in wild birds and changes in environmental habitat: a relationship to be considered.any human activity, even if aimed at the improvement of a natural area, can potentially affect wildlife, leading to possible short-term or long-term changes due to the human-wildlife interaction. in this study, a botulism outbreak which occurred in waterfowl in a nature reserve after a conservative environmental action is reported. more than 180 different species of wild birds, including seventy waterfowl species, live in the area. the wildlife reserve rangers built an artificial pond equipped w ...201931779284
full-length transcriptome sequencing from multiple tissues of duck, anas platyrhynchos.duck (anas platyrhynchos), one of the most economically important waterfowl, is an ideal model for studying the immune protection mechanism of birds. an incomplete duck reference genome and very limited availability of full-length cdnas has hindered the identification of alternatively spliced transcripts and slowed down many basic studies in ducks. we applied pacbio iso-seq technologies to multiple tissues from duck for use in transcriptome sequencing. we obtained 199,993 full-length transcripts ...201931754106
genome sequence of a thermostable avirulent newcastle disease virus isolated from domestic ducks in china.newcastle disease virus strain d4 was isolated from healthy ducks in hubei, china. the d4 isolate has a genome length of 15,186 nucleotides and is classified as genotype i of class ii. thermostability and pathogenicity tests demonstrate that d4 is a thermostable avirulent strain.201931753945
identification of carbon nanotube particles in liver tissue and its effects on apoptosis of birds exposed to air pollution.this study aimed to distinguish carbon nanotube (cnt) particles and their pathological effects on the liver of birds in areas with carbon emissions.201931749569
a novel neuraminidase-dependent hemagglutinin cleavage mechanism enables the systemic spread of an h7n6 avian influenza virus.in this study, we demonstrate a novel mechanism for hemagglutinin (ha) activation in a naturally occurring h7n6 avian influenza a virus strain, a/mallard duck/korea/6l/2007 (a/mdk/6l/07). this novel mechanism allows for systemic infection of chickens, ducks, and mice, and a/mdk/6l/07 can replicate in vitro without exogenous trypsin and exhibits broad tissue tropism in animals despite the presence of a monobasic ha cleavage motif (peipkgr/g). the trypsin-independent growth phenotype requires the ...201931690675
integrated analysis of mrna expression, cpg island methylation, and polymorphisms in the mitf gene in ducks (anas platyrhynchos).microphthalmia-associated transcription factor (mitf) is a key regulator for the development and function of melanocytes in skin, eye, and plumage pigmentations. thus, the mitf was selected as a candidate gene associated with plumage coloration in ducks. this study analyzed the mrna expression, promoter methylation, and polymorphisms in the mitf gene in ducks with different plumage colors (putian black, putian white, liancheng white, and longsheng jade-green). no expression of the mitf melanin-s ...201931662999
molecular cloning, characterization, and temporal expression profile of troponin i type 1 (tnni1) gene in skeletal muscle during early development of gaoyou duck (anas platyrhynchos domestica).tnni1 encodes the slow skeletal muscle isoform of troponin i. in the present study, the basic characteristic and expressing profile of the tnni1 gene was first explored in gaoyou ducks. full-length tnni1 cdna of gaoyou duck was obtained using reverse transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). the cdna consisted of a 57-base pair (bp) 5'utr, a 345-bp 3'utr, and a 564-bp open reading frame. the predicted protein was predicted to be hydrophilic, no ...201929557225
meteorological factors affecting the risk of transmission of hpai in miyazaki, japan.highly pathogenic avian influenza (hpai) outbreaks engender a severe economic impact on the poultry industry and public health. migratory waterfowl are considered the natural hosts of hpai virus, and hpai viruses are known to be transmitted over long distances during seasonal bird migration. bird migration is greatly affected by the weather. many studies have shown the relationship between either autumn or spring bird migration and climate. however, few studies have shown the relationship betwee ...201931565229
embryonic development of parakeratinized epithelium of the tongue in the domestic duck (anas platyrhynchos f. domestica): lm, sem, and tem observations.the parakeratinized epithelium is a common and widespread type of keratinized epithelium in the oral cavity in adult birds. in contrast to orthokeratinized epithelium, which mostly covers mechanical papillae and the lingual nail, parakeratinized epithelium covers almost the entire dorsal surface of the tongue in birds. the characteristic feature of parakeratinized epithelium is the presence of nuclei in the keratinized layer. the present study aimed to investigate for the first time the micro- a ...201930382421
identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activity.this study was conducted to identify duck liver-expressed antimicrobial peptide 2 (leap-2) and demonstrate its antimicrobial activity against various pathogens.201930381731
long-term passage of duck tembusu virus in bhk-21 cells generates a completely attenuated and immunogenic population with increased genetic diversity.duck tembusu virus (tmuv) is an emerging pathogenic flavivirus that causes severe egg-drop and fatal encephalitis in domestic ducks and geese. although a live-attenuated virus vaccine is effective for disease control, the stability of the attenuation has not been clearly evaluated due to a poor understanding of the attenuation mechanism. here, a virulent duck tmuv isolate was successively passaged in bhk-21 cells, leading to an approximately 100-fold increase of virus production in cell culture ...201931708180
development and validation of a universal one-step rt-pcr assay for broad detection of duck tembusu virus.duck tembusu virus (dtmuv), a mosquito-borne flavivirus, has been identified as a causative agent of an emerging disease in ducks. since its first report in 2010, several clusters of dtmuv have increasingly been identified and caused outbreaks in many asian countries. this highlights the need for improved and novel broad detection assays in order to detect all circulating clusters of dtmuv. in this study, a universal one-step reverse-transcription pcr (rt-pcr) assay targeting a highly conserved ...202033205167
the evolutionary dynamics of influenza a viruses circulating in mallards in duck hunting preserves in maryland, usa.duck hunting preserves (dhp) have resident populations of farm-raised mallard ducks, which create potential foci for the evolution of novel influenza a viruses (iavs). through an eleven-year (2003-2013) iav surveillance project in seven dhps in maryland, usa, we frequently identified iavs in the resident, free-flying mallard ducks (5.8% of cloacal samples were iav-positive). the iav population had high genetic diversity, including 12 ha subtypes and 9 na subtypes. by sequencing the complete geno ...202033375548
a comparative study of the innate humoral immune response to avian influenza virus in wild and domestic mallards.domestic mallards (anas platyrhynchos domesticus) are traditionally used as a model to investigate infection dynamics and immune responses to low pathogenic avian influenza viruses (lpaivs) in free-living mallards. however, it is unclear whether the immune response of domestic birds reflects the response of their free-living counterparts naturally exposed to these viruses. we investigated the extent to which the innate humoral immune response was similar among (i) wild-type domestic mallards in ...202033329501
pattern-recognition receptors in duck (anas platyrhynchos): identification, expression and function analysis of toll-like receptor 3.1. innate immunity provides the first line of defence against pathogenic organisms through a myriad of germline encoded receptors called pattern-recognition receptors (prrs). toll-like receptor (tlr) 3, as an important member of prrs, is indispensable for host defence against viral infection by recognising virus-derived rnas. however, little is known about the structure and function of tlr3 in ducks (anas platyrhynchos), a natural host for the avian influenza virus.2. this study cloned the full- ...202033215508
prevalence and distribution of avian influenza viruses in domestic ducks at the waterfowl-chicken interface in wetlands.ducks are a natural reservoir of influenza a viruses (iavs) and can act as a reassortment vessel. wetlands, such as hakaluki and tanguar haor in bangladesh, have unique ecosystems including domestic duck (anas platyrhynchos domesticus) rearing, especially household and free-range ducks. a cross-sectional study was, therefore, conducted to explore avian influenza status and its distribution and risk factors in the wetland areas. during the three consecutive winters of 2015-2017, specifically in d ...202033207803
truncation or deglycosylation of the neuraminidase stalk enhances the pathogenicity of the h5n1 subtype avian influenza virus in mallard ducks.h5n1 subtype avian influenza virus (aiv) with a deletion of 20 amino acids at residues 49-68 in the stalk region of neuraminidase (na) became a major epidemic virus. to determine the effect of truncation or deglycosylation of the na stalk on virulence, we used site-directed mutagenesis to insert 20 amino acids in the short-stalk virus a/mallard/huadong/s/2005 (sy) to recover the long-stalk virus (rsna+). a series of short-stalk or deglycosylated-stalk viruses were also constructed basing on the ...202033193225
genetic evolution and transmission dynamics of clade 2.3.2.1a highly pathogenic avian influenza a/h5n1 viruses in bangladesh.asian lineage a/h5n1 highly pathogenic avian influenza viruses (hpaivs) have been responsible for continuous outbreaks in bangladesh since 2007. although clades 2.2.2 and 2.3.4.2 hpaivs have disappeared since poultry vaccination was introduced in 2012, clade 2.3.2.1a viruses have continued to be detected in bangladesh. in this study, we identified a/h9n2 (n = 15), a/h5n1 (n = 19), and a/h5n1-a/h9n2 (n = 18) mixed viruses from live bird markets, chicken farms, and wild house crows (corvus splende ...202034127940
evaluation of threaded external skeletal fixation pin insertion with and without predrilling in avian bone.the aim of this study was to document the effects of positive-profile threaded external skeletal fixation (esf) pin insertion without predrilling on heat generation, structural damage and pullout strength in avian bone than that with predrilling.202031618774
domestic ducks play a major role in the maintenance and spread of h5n8 highly pathogenic avian influenza viruses in south korea.the h5n8 highly pathogenic avian influenza viruses (hpaivs) belonging to clade 2.3.4.4 spread from eastern china to korea in 2014 and caused outbreaks in domestic poultry until 2016. to understand how h5n8 hpaivs spread at host species level in korea during 2014-2016, a bayesian phylogenetic analysis was used for ancestral state reconstruction and estimation of the host transition dynamics between wild waterfowl, domestic ducks and chickens. our data support that h5n8 hpaiv most likely transmitt ...202031675474
inter genotype differences in expression of genes involved in glucose metabolism in the establishment of hepatic steatosis in muscovy, pekin and mule ducks.in waterfowls, overfeeding leads to a hepatic steatosis, also called "foie gras". our main objectives were to determine what is the share of genes involvement of glucose metabolism in the establishment of fatty liver in three genotypes of waterfowls: muscovy (cairina moschata), pekin ducks (anas platyrhynchos) and their crossbreed, the mule duck. 288 male ducks of pekin, muscovy and mule genotypes were reared until weeks 12 and overfed between weeks 12 and 14. we analysed gene expression at the ...202031741265
cellular elements organization in the trachea of mallard (anas platyrhynchos) with a special reference to its local immunological role.many studies have been carried out to investigate the histological structure of the trachea in many species of birds. however, the cellular organization of the trachea in the mallard duck is still unclear. this study was performed on 12 sexually mature male mallard duck to demonstrate the cellular organization of the trachea using light and electron microscopy. the tracheal epithelium is considered the first line of defense against airborne pathogens. the mallard trachea was lined by a pseudostr ...202031724070
comparison of immunohistochemistry and ziehl-neelsen staining for detecting the distribution of mycobacterium avium subsp avium in naturally infected domestic pekin ducks (anas platyrhynchos domestica).in order to detect the distribution of mycobacterium avium subsp avium (maa) in naturally infected domestic pekin ducks, immunohistochemistry (ihc) and ziehl-neelsen (zn) staining were used and compared. six organs, the liver, spleen, lung, kidney, duodenum and pectoralis muscle, were collected from naturally infected pekin ducks. paraffin embedded tissues were examined, and the results were compared. statistical analysis was performed using chi-square test. the results showed that the detection ...202031770824
genetic and antigenic characterization of the first h7n7 low pathogenic avian influenza viruses isolated in vietnam.during the annual surveillance of avian influenza viruses (aivs) in vietnam in 2018, three h7n7 aiv isolates were identified in domestic ducks in a single flock in vinh long province. the present study is the first documented report of h7n7 virus isolates in vietnam and aimed to characterize these viruses, both genetically and antigenically. deduced amino acid sequences for the hemagglutinins (has) indicated a low pathogenicity of these viruses in chickens. phylogenetic analysis revealed that th ...202031760087
integrated taxonomic approaches to seven species of capillariid nematodes (nematoda: trichocephalida: trichinelloidea) in poultry from japan and indonesia, with special reference to their 18s rdna phylogenetic relationships.morphological and genetic analyses were performed on seven species of the family capillariidae (nematoda: trichocephalida: trichinelloidea), viz. eucoleus perforans, eucoleus contortus, aonchotheca bursata, baruscapillaria obsignata, capillaria anatis, capillaria phasianina, and capillaria spinulosa, detected in poultry from japan and indonesia. two eucoleus spp., perforating the esophageal mucosa of the japanese green pheasant farmed in japan (e. perforans) and domestic goose in indonesia (e. c ...202031811424
morphological comparison of genetically differentiated polymorphus cf. minutus types.in the present study, we analyzed the morphology of three genetic types of the bird-infecting acanthocephalan polymorphus cf. minutus (pspt1, pspt2, pspt3), mainly based on the cystacanth-stage obtained from amphipods (gammarus fossarum, gammarus pulex, gammarus roeselii, echinogammarus spp.). males and females were pooled as there was no considerable difference between the sexes concerning the hook measurements. additionally, we conducted a laboratory infection of one domestic duck for each pol ...202031786696
geographic and ecologic aspects of the community structure of trematodes of mallards (anas platyrhynchos) in northern poland and the czech republic.trematode infestation of mallards (anas platyrhynchos) varies between northern poland and the czech republic. we determined the trematode fauna of juvenile and adult mallards from poland in 2010-16 (n=79 mallards) and from the czech republic in 1964-2001 (n=296). infracommunity diversity of trematodes from juvenile mallards, defined by the brillouin index and mean number of species, was significantly greater in poland than it was in the czech republic. there were more species and greater biodive ...202031895644
assessing changes in genomic divergence following a century of human-mediated secondary contact among wild and captive-bred ducks.along with manipulating habitat, the direct release of domesticated individuals into the wild is a practice used worldwide to augment wildlife populations. we test between possible outcomes of human-mediated secondary contact using genomic techniques at both historical and contemporary timescales for two iconic duck species. first, we sequence several thousand ddrad-seq loci for contemporary mallards (anas platyrhynchos) throughout north america and two domestic mallard types (i.e., known game-f ...202031872482
development of a simple and rapid immunochromatographic strip test for detecting duck plague virus antibodies based on gi protein.a colloidal gold strip (cgs) for detecting antibodies to duck plague virus (dpv) was developed. colloidal gold-labeled dpv gi protein and goat anti-rabbit igg were dispensed on a conjugate pad as tracers. the recombinant dpv gi protein and rabbit igg were used as capture reagents at the test line and control line, respectively. the detection limit of this assay was 1:256. additionally, the cgs did not react with antisera from other common duck diseases, only reacting with anti-dpv serum and yiel ...202031863862
an efficient method for collecting the full-length adults, fragments, and eggs of trichobilharzia spp. from the liver of definitive hosts.precise identification of avian schistosomes in the genus trichobilharzia at the species level is difficult and requires both traditional morphological and molecular techniques. to obtain satisfactory results by traditional methods, the characteristics of the intact adults or large fragments of male and females are necessary. the present study aimed to introduce a more efficient method for collecting eggs and both fragments and intact worms for morphological identification of visceral trichobilh ...202031863180
molecular cloning, expression and mimicking antiviral activity analysis of retinoic acid-inducible gene-i in duck (anas platyrhynchos).intracellular double-stranded rna (dsrna) is a chief sign of replication for many viruses. pattern recognition receptors(prrs) of the innate immune system detected the dsrna and initiate the antiviral responses. retinoic acid-inducible gene i (rig-i), a member of prrs, plays an essential regulatory role in dsrna-induced signalling. in this study, the full-length complementary dna (cdna) of duck rig-i (durig-i) was cloned using the reverse transcription-polymerase chain reaction (rt-pcr) and rapi ...202032366736
high-temperature exposure alters the community structure and functional features of the intestinal microbiota in shaoxing ducks (anas platyrhynchos).the gut microbiome is a complex ecosystem that contributes to host nutrition and health. however, our current knowledge of the relationship between ambient temperature and gut microbiota of poultry is still limited. the objective of the present study was to characterize the intestinal microbiota of ducks exposed to high ambient temperature. sixty 60-day-old shaoxing ducks were allocated to control and heat-treated groups. the ducks in the control group were kept at 25°c, and the ducks in the hea ...202032359603
feathers of three waterfowl bird species from northern iran for heavy metals biomonitoring.waterbirds are considered the effective sentinels of aquatic ecosystems contamination and their feathers are widely used as non-destructive biological tools for contaminant biomonitoring. in this work, we measure and evaluate the concentrations of lead, copper and zinc in the breast feathers of three species namely fulica atra, anas crecca and anas platyrhynchos in the wetland national park boujagh. levels of lead (pb), copper (cu) and zinc (zn) were below the threshold that causes sub-lethal an ...202032333043
active virological surveillance in backyard ducks in bangladesh: detection of avian influenza and gammacoronaviruses.domestic waterfowl play an important role in the perpetuation and transmission of avian pathogens including avian influenza viruses (aiv) of low and high pathogenicity, which pose severe economic and public health concerns in bangladesh. this study focused on active surveillance of several avian viral pathogens with a special reference to aiv in selected backyard duck populations in bangladesh. a total of 500 pooled oropharyngeal and cloacal samples from individual ducks of four districts were t ...202032271094
timing, frequency, and duration of incubation recesses in dabbling ducks.nest attendance is an important determinant of avian reproductive success, and identifying factors that influence the frequency and duration of incubation recesses furthers our understanding of how incubating birds balance their needs with those of their offspring. we characterized the frequency and timing (start time, end time, and duration) of incubation recesses for mallard (anas platyrhynchos) and gadwall (mareca strepera) hens breeding in suisun marsh, california, usa, and examined the infl ...202032184998
live bird markets as evolutionary epicentres of h9n2 low pathogenicity avian influenza viruses in korea.live bird markets (lbms) in korea have been recognized as a reservoir, amplifier, and source of avian influenza viruses (aivs); however, little was known about the role of lbms in the epidemiology of aivs in korea until recently. through 10 years of surveillance (2006-2016) we have isolated and sequenced h9n2 viruses in korean lbms. to understand how h9n2 evolves and spreads in korea, a statistical bayesian phylogenetic model was used. phylogenetic analysis suggests that three separate introduct ...202032183621
a brief introduction to avian influenza virus.the earliest recorded cases of what was likely high-pathogenicity aiv in poultry were reported in italy in the 1870s. avian influenza infection has been recognized in domestic poultry through the modern era of poultry production. infection of poultry with either low pathogenic (lp) or highly pathogenic (hp) avian influenza viruses (aivs) can result in substantial economic consequences. productivity can be reduced directly and indirectly because of disease leading to decreased egg or meat yield, ...202032170682
understanding the genetic domestication history of the jianchang duck by genotyping and sequencing of genomic genes under selection.the jianchang duck is mainly distributed in southwest china, and has the characteristics of fast growth rate and strong abilities in lipid deposition in the liver. in order to investigate the effects of domestication process on formation of the unique characteristics of jianchang duck, the whole genome of sixteen individuals and three pooling of jianchang duck were re-sequenced, and genome data of 70 mallards and 83 domestic ducks from thirteen different places in china were obtained from ncbi. ...202032165372
molecular survey of duck circovirus infection in poultry in southern and southwestern china during 2018 and 2019.duck circovirus (ducv) is a potential immunosuppressive virus that causes feather disorders in young ducks. in this study, ducv obtained from various species of ducks was investigated by polymerase chain reaction (pcr) in southern and southwestern china (guangdong, guangxi and yunnan provinces) from 2018 to 2019.202032138728
seed mass, hardness, and phylogeny explain the potential for endozoochory by granivorous waterbirds.field studies have shown that waterbirds, especially members of the anatidae family, are major vectors of dispersal by endozoochory for a broad range of plants lacking a fleshy fruit, yet whose propagules can survive gut passage. widely adopted dispersal syndromes ignore this dispersal mechanism, and we currently have little understanding of what traits determine the potential of angiosperms for endozoochory by waterbirds. results from previous experimental studies have been inconsistent as to h ...202032076524
detection of fowl adenovirus d strains in wild birds in poland by loop-mediated isothermal amplification (lamp).the present study on the role of strains of adenovirus in wildlife reservoirs, and their prevalence is under exploration. in several previous studies, the presence of adenovirus strains in wild birds has been investigated. worldwide distribution and outbreaks of adenovirus infections have been reported by many authors. the present study investigated the prevalence of fadvs in 317 samples of different bird species from the northwestern region of poland. an applied specific, sensitive, and efficie ...202032059679
modulation of lethal hpaiv h5n8 clade 2.3.4.4b infection in aiv pre-exposed mallards.in 2016/2017, a severe epidemic of hpaiv h5n8 clade 2.3.4.4 group b (h5n8b) affected europe. to analyse the role of mallards in the spatiotemporal dynamics of global hpaiv h5n8b dispersal, mallards (anas platyrhynchos), naturally exposed to various aiv and therefore seropositive, were challenged with h5n8b. all experiments were controlled by infection and co-housing of seronegative juvenile pekin ducklings. all ducks that survived the first infection were re-challenged 21 dpi with the homologous ...202031969057
dynamic accumulation of fatty acids in duck (anas platyrhynchos) breast muscle and its correlations with gene expression.fatty acid composition contributes greatly to the quality and nutritional value of meat. however, the molecular regulatory mechanisms underlying fatty acid accumulation in poultry have not yet been cleared. the aims of this study were to characterize the dynamics of fatty acid accumulation in duck breast muscle and investigate its correlations with gene expression.202031952469
outbreaks of highly pathogenic avian influenza in zoo birds caused by ha clade 2.3.4.4 h5n6 subtype viruses in japan in winter 2016.in late 2016, two zoos, one in northern japan and the other in central japan, experienced highly pathogenic avian influenza (hpai) outbreaks, in which multiple zoo birds were infected with h5n6 subtype hpai virus (hpaiv). here, we report an overview of these hpai outbreaks. hpaiv infections were confirmed by virus isolation in three black swans (cygnus atratus) and three snowy owls (bubo scandiacus) kept in the omoriyama zoo hospital. at higashiyama zoo and botanical gardens, following the death ...202031605424
systemic collapse of a host-parasite trematode network associated with wetland birds in europe.as host community diversity decreases, parasite diversity may also decline. the life cycles of trematodes involve multiple hosts from different orders, with many trematodes displaying narrow host specialization. in the 1960s and 2010s, we performed full-body necropsies of juvenile or first-year birds of four wetland bird species, anas platyrhynchos, aythya fuligula, fulica atra, and chroicocephalus ridibundus which originated from the southern czech republic, and examined them for the presence o ...202032088756
prevalence and potential risk of salmonella enterica in migratory birds from south korea.salmonella is a type of zoonotic bacteria that represents an economic and public health concern worldwide. difficulties in sample collection from migratory birds mean little is known about their importance as a reservoir of salmonella. the present study evaluated the prevalence and potential risk of salmonella enterica in migratory birds. from 2012-2017, 3661 cloacal swabs from migratory birds were collected in south korea and tested to isolate s. enterica. strains were tested for antimicrobial ...202032980630
does the renal portal valve exist in a raptor species? a study aimed at further evaluating the mechanism of toxicity of diclofenac in vultures.diclofenac has been responsible for the deaths of millions of vultures on the asian subcontinent. while the pathology of toxicity is well described, the mechanism of toxicity remains elusive. however, it was postulated that toxicity could be related to the unique avian renal vascular structure known as the renal portal valve and that that diclofenac altered valve functionality with subsequent renal ischaemia. while plausible, the valva renalis portalis has only been described in a small number o ...202032608095
biogeography of microbiome and short-chain fatty acids in the gastrointestinal tract of duck.it is generally accepted that domestic ducks are valuable protein sources for humans. the gastrointestinal ecosystem contains enormous and complicated microbes that have a profound effect on the nutrition, immunity, health, and production of domestic ducks. to deeply understand the gastrointestinal microbial composition of domestic ducks, we investigated the microbiomes of 7 different gastrointestinal locations (proventriculus, gizzard, duodenum, jejunum, ileum, cecum, and rectum) and the short- ...202032731989
phenotypic and genotypic properties of vibrio cholerae non-o1, non-o139 isolates recovered from domestic ducks in germany.vibrio cholerae non-o1, non-o139 bacteria are natural inhabitants of aquatic ecosystems and have been sporadically associated with human infections. they mostly lack the two major virulence factors of toxigenic v. cholerae serogroups o1 and o139 strains, which are the causative agent of cholera. non-o1, non-o139 strains are found in water bodies, sediments, and in association with other aquatic organisms. occurrence of these bacteria in fecal specimens of waterfowl were reported, and migratory b ...202032717968
year‑round influenza a virus surveillance in mallards (anas platyrhynchos) reveals genetic persistence during the under‑sampled spring season.active influenza a virus (iav) surveillance in wild waterfowl in the united states has revolved around convenience-based sampling methods, resulting in gaps in surveillance during the spring season. we conducted active iav surveillance in mallards continuously from july 2017 to july 2019 in the coastal marshes of lake erie near port clinton, ohio. we aimed to understand ecological and evolutionary dynamics of iav across multiple seasons, including the under‑sampled spring season. we collected 20 ...202032545281
pattern recognition receptor signaling and innate responses to influenza a viruses in the mallard duck, compared to humans and chickens.mallard ducks are a natural host and reservoir of avian influenza a viruses. while most influenza strains can replicate in mallards, the virus typically does not cause substantial disease in this host. mallards are often resistant to disease caused by highly pathogenic avian influenza viruses, while the same strains can cause severe infection in humans, chickens, and even other species of ducks, resulting in systemic spread of the virus and even death. the differences in influenza detection and ...202032477965
co-circulation of multiple reassortant h6 subtype avian influenza viruses in wild birds in eastern china, 2016-2017.h6 subtype influenza viruses were prevalent in domestic poultry and wild birds, which also could pose potential threat to humans. however, little is known about the prevalence of h6 subtype viruses in wild birds in eastern china, a crucial stopover or wintering site for migratory wild birds along the east asian-australasian flyway.202032349760
the specificity protein 3 (sp3) gene in ducks (anas platyrhynchos): cloning, characterization and expression during viral infection.specificity protein 3 (sp3) is a newly identified regulator of tumor growth and invasiveness in humans. in this study, we identified and characterized the function of duck sp3 (dusp3). the full-length cdna sequence of the dusp3 gene was cloned via rapid amplification of cdna ends. it contained 2468 nucleotides, including a 111 base pair (bp) 5'-untranslated region (utr), 215 bp 3'-utr, and 2142 bp open reading frame (orf), which encoded a 713 amino acid (aa) strongly conserved with avian sp3. ti ...202032180490
quantifying within-host diversity of h5n1 influenza viruses in humans and poultry in cambodia.avian influenza viruses (aivs) periodically cross species barriers and infect humans. the likelihood that an aiv will evolve mammalian transmissibility depends on acquiring and selecting mutations during spillover, but data from natural infection is limited. we analyze deep sequencing data from infected humans and domestic ducks in cambodia to examine how h5n1 viruses evolve during spillover. overall, viral populations in both species are predominated by low-frequency (<10%) variation shaped by ...202031951644
the association between saα2,3gal occurrence frequency and avian influenza viral load in mallards (anas platyrhynchos) and blue-winged teals (spatula discors).individual heterogeneity in pathogen load can affect disease transmission dynamics; therefore, identifying intrinsic factors responsible for variation in pathogen load is necessary for determining which individuals are prone to be most infectious. because low pathogenic avian influenza viruses (lpaiv) preferentially bind to alpha-2,3 sialic acid receptors (saα2,3gal) in the intestines and bursa of fabricius in wild ducks (anas and spatula spp.), we investigated juvenile mallards (anas platyrhync ...202033167978
transcriptome reveals multi pigmentation genes affecting dorsoventral pattern in avian body.certain animals exhibit a special dorsoventral pattern with a lighter ventral side compared to the dorsal one and this phenomenon was preserved in the long-term evolution process. birds also retain this trait. recently, inaba et al. (2019) found that asip (agouti signal protein) regulated interconversion between different melanocyte types leads to dorsal stripe pattern, which may partly explain the birds' dorsoventral plumage color difference. in this study, we used the embryo samples of lbm (li ...202033117797
pathogenicity of a jinding duck-origin cluster 2.1 isolate of tembusu virus in 3-week-old pekin ducklings.tembusu virus (tmuv) infection most commonly affects breeder and layer ducks during laying period, and can also affect young ducks below 7 weeks of age. here, we report our investigation of a tmuv-caused fatal disease of jingding ducklings (anas platyrhynchos domesticus) in northeast china. the disease resulted in mortalities of up to 40 % in 2 to 4-week-old ducks, up to 25 % in 5 to 6-week-old ducks, and less than 10 % in 7 to 8-week-old ducks. using a tmuv-specific reverse transcription-pcr as ...202033053451
manipulating plasma thyroid hormone levels at hatching alters development of endothermy and ventilation in pekin duck (anas platyrhynchos domestica).at hatching in precocial birds, there are rapid physiological and metabolic phenotypic changes associated with attaining endothermy. during the transition to ex ovo life, thyroid hormone levels naturally increase, peaking at hatching, and then declining. to better understand the role of the natural increase in thyroid hormone at hatching in regulating the developmental trajectory of the pekin duck's endothermic phenotype, we examined development of o2 consumption (v̇o2) and ventilation (frequenc ...202033046566
genetic signature of hybridization between chinese spot-billed ducks and domesticated ducks.in this study, we analyzed 93 whole genomes from chinese spot-billed ducks (csb), meat-type ducks (met), and egg and dual purpose-type ducks (edt) to characterize the genetic material flowing between the csb and modern ducks. using a frequency of shared identical-by-descent method, approximately 10.9 mb introgression segments containing 140 genes were identified showing the signatures of introgression between csb and edt. meanwhile, nearly 10.6 m introgression regions containing 149 genes were i ...202033020910
natural and experimental persistence of highly pathogenic h5 influenza viruses in slurry of domestic ducks, with or without lime treatment.infections by a/h5 and a/h7 avian influenza viruses (aivs) can cause acute disease and are therefore notifiable in poultry and wild birds. during winter 2015-2016, several cases of infection caused by highly pathogenic (hp) aivs belonging to the a/h5n1, a/h5n2 and a/h5n9 subtypes, were detected in south-western france. throughout winter 2016-2017, several cases of infections mainly caused by a/h5n8 hp aiv (a/gooses/gd/1/96 clade 2.3.4.4) were detected across europe. on both occasions, the viruse ...202033008818
development of a relevant strategy using de novo transcriptome assembly method for transcriptome comparisons between muscovy and common duck species and their reciprocal inter-specific mule and hinny hybrids fed ad libitum and overfed.common pekin and muscovy ducks and their intergeneric hinny and mule hybrids have different abilities for fatty liver production. rna-seq analyses from the liver of these different genetic types fed ad libitum or overfed would help to identify genes with different response to overfeeding between them. however rna-seq analyses from different species and comparison is challenging. the goal of this study was develop a relevant strategy for transcriptome analysis and comparison between different spe ...202033008290
free-ranging avifauna as a source of generalist parasites for captive birds in zoological settings: an overview of parasite records and potential for cross-transmission.captive birds in zoological settings often harbor parasites, but little information is available about the potential for free-ranging avifauna to act as a source of infection. this review summarizes the gastrointestinal parasites found in zoo birds globally and in seven common free-ranging avian species [mallard (anas platyrhynchos), eurasian blackbird (turdus merula), common starling (sturnus vulgaris), eurasian jackdaw (corvus monedula), house sparrow (passer domesticus), european robin (erith ...202033005675
characterization of avian influenza virus h10-h12 subtypes isolated from wild birds in shanghai, china from 2016 to 2019.h10, h11 and h12 (h10-h12) subtypes of the avian influenza virus (aiv) are associated with waterfowl. although these subtypes of aiv are infrequently detected in nature, they can undergo reassortment with other aiv subtypes. few h10-h12 subtypes of aiv have been isolated from wild birds in china. in this study, 12 aiv isolates of h10-h12 subtypes were identified via routine surveillance of wild birds in shanghai, china from 2016 to 2019, including two h10, three h11 and seven h12 isolates. seque ...202032992999
reproductive season and male effect on quantitative and qualitative traits of individually collected muscovy duck (cairina moschata) semen.males of muscovy duck (cairina moschata) are mainly used for mule duck production via artificial insemination of females originated from wild mallard duck (anas platyrhynchos); therefore, the quantity and quality of drake semen play a crucial role. the assessment results of male reaction to sexual stimulation by dummy female and basic semen characteristic (ejaculate volume, sperm concentration and morphology) of 12 individually kept muscovy drakes carried out during the entire reproductive seaso ...202032989837
potential reverse spillover of infectious bursal disease virus at the interface of commercial poultry and wild birds.recently, multiple spillover events between domesticated poultry and wild birds have been reported for several avian viruses. this phenomenon highlights the importance of the livestock-wildlife interface in the possible emergence of novel viruses. the aim of the current study was to investigate the potential spillover and epidemiological links of infectious bursal disease virus (ibdv) between wild birds and domestic poultry. to this end, twenty-eight cloacal swabs were collected from four specie ...202032970296
slight volume changes in the duck lung do not imply a fundamental change in the structure of the parenchyma.slight changes in lung volume have previously been reported in ducks. we studied the functional structure of the lung of the domestic duck using classical anatomical techniques as well as ultrasound monitoring to unravel the causes of such changes. later dorsal and medioventral secondary bronchi were superficially positioned and covered with a thin transparent and collapsible membrane, internally lined with a cuboidal to squamous epithelium. the lung parenchyma was rigid, with atria well support ...202032969516
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