Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| studies on antimicrobial and immunomodulatory effects of hot aqueous extract of acacia nilotica l. leaves against common veterinary pathogens. | acacia nilotica is a plant species that is almost ubiquitously found in different parts of the world. various preparations of it have been advocated in folk medicine for the treatment of tuberculosis, leprosy, smallpox, dysentery, cough, ophthalmia, toothache, skin cancer as astringent, antispasmodic, and aphrodisiac since immemorial times. the present study investigates the antibacterial, antifungal, antiviral, and immunomodulatory potential of hot aqueous extract (hae) of acacia nilotica leave ... | 2014 | 24804150 |
| draft genome sequences of two bacillus thuringiensis strains and characterization of a putative 41.9-kda insecticidal toxin. | in this work, we report the genome sequencing of two bacillus thuringiensis strains using illumina next-generation sequencing technology (ngs). strain hu4-2, toxic to many lepidopteran pest species and to some mosquitoes, encoded genes for two insecticidal crystal (cry) proteins, cry1ia and cry9ea, and a vegetative insecticidal protein (vip) gene, vip3ca2. strain leapi01 contained genes coding for seven cry proteins (cry1aa, cry1ca, cry1da, cry2ab, cry9ea and two cry1ia gene variants) and a vip3 ... | 2014 | 24784323 |
| characterization of a novel manduca sexta beta-1, 3-glucan recognition protein (βgrp3) with multiple functions. | recognition of pathogens by insect pattern recognition receptors is critical to mount effective immune responses. in this study, we reported a new member (βgrp3) of the β-1, 3-glucan recognition protein (βgrp) family from the tobacco hornworm manduca sexta. unlike other members of the m. sexta βgrp family proteins, which contain an n-terminal small glucan binding domain and a c-terminal large glucanase-like domain, βgrp3 is 40-45 residues shorter at the n-terminus and lacks the small glucan bind ... | 2014 | 24952171 |
| design, synthesis, antimicrobial activity and anti-hiv activity evaluation of novel hybrid quinazoline-triazine derivatives. | a series of novel hybrid quinazoline-triazine derivatives was designed and synthesized from cyanuric chloride and anthranilic acid through sequential reactions, which contain different pharmacophores like quinazoline and substituted diaryl triazine (data) linked with ethylene diamine. all the newly synthesized compounds were characterized by infrared, (1)h-nmr, (13)c-nmr, ms and elemental analysis. further, we evaluated the in vitro anti-hiv activity of the newly synthesized compounds against hi ... | 2014 | 23327639 |
| antibacterial, antifungal and antioxidant activities of honey collected from timergara (dir, pakistan). | in this study honeys of acacia modesta, prunus persica, zizyphus sativa and isodon rogosus plants were tested against two gram-positive bacterial strains (staphylococcus aureus and bacillus cereus), two gram-negative bacterial strains (klebsilla pneumonia and escherichia coli) and two fungal strains (alternaria alternata and trichoderma harzianum) through agar well diffusion method. the tested honeys showed high antimicrobial activities to the tested bacterial and fungal strains. all the tested ... | 2014 | 24374434 |
| [evaluation of endoscopy assisted vitrectomy for the treatment of severe traumatic eyes with no light perception]. | to evaluate the effect of endoscopy assisted pars plana vitrectomy for severe ocular trauma with no light perception. | 2014 | 24841815 |
| antibiotic-resistant heterotrophic plate count bacteria and amoeba-resistant bacteria in aquifers of the mooi river, north west province, south africa. | groundwater in the mooi river catchment is prone to mining, agricultural, municipal and septic tank pollution. in this study physico-chemical and microbiological parameters were determined using appropriate methods. bacterial isolates were identified by 16s rrna sequencing (heterotrophic plate count (hpc) bacteria and amoeba-resistant bacteria (arb)) and multiplex polymerase chain reaction (escherichia coli). antibiotic resistance tests were also performed. physico-chemical parameters were gener ... | 2014 | 25473993 |
| antimicrobial and anti-inflammatory activities of pleurostylia capensis turcz (loes) (celastraceae). | pleurostylia capensis is a large tree that can reach the maximum height of 20 m long, and it have been traditionally used as cosmetic, for steam bath, ritual body wash, and as a purgative to treat symptoms of witchcraft. using ethanol, chloroform, dichloromethane (dcm), ethyl acetate (ea), and water extracts, leaves, bark and roots of pleurostylia capensis were investigated scientifically for their effectiveness in antimicrobial, antioxidant and anti-inflammatory activities using standard method ... | 2014 | 25435632 |
| chemical constituents and antimicrobial properties of the essential oil and ethanol extract from the stem of aglaia odorata lour. | the stem-derived essential oil of aglaia odorata lour. was obtained by hydrodistillation using a clevenger-type system. gas chromatography-mass spectrometry analysis of the oil revealed the identification of 39 compounds, representing 76.4% of the oil; germacrene d (20.3%), α-humulene (17.1%), α-himachalene (12.7%) and β-caryophyllene (10.2%) were the major components. ar-turmerone (1) and eichlerialactone (2) were isolated from the stem oil and ethanolic stem extract of this plant species, resp ... | 2014 | 24934340 |
| safety, efficacy and utility of methods of transferring adhesive and cohesive escherichia coli cells to microplates to avoid aerosols. | the virtual colony count (vcc) microbiological assay has been utilized for over a decade to measure the antimicrobial activity of peptides such as defensins and ll-37 against biosafety level (bsl)-1 and bsl-2 bacteria including escherichia coli, staphylococcus aureus, bacillus cereus, and enterobacter aerogenes. in addition, a modified pipetting technique was presented in a 2011 study of defensin activity against the bsl-3 pathogen bacillus anthracis. both studies were published in the journal ... | 2014 | 25671086 |
| virulence plasmids of spore-forming bacteria. | plasmid-encoded virulence factors are important in the pathogenesis of diseases caused by spore-forming bacteria. unlike many other bacteria, the most common virulence factors encoded by plasmids in clostridium and bacillus species are protein toxins. clostridium perfringens causes several histotoxic and enterotoxin diseases in both humans and animals and produces a broad range of toxins, including many pore-forming toxins such as c. perfringens enterotoxin, epsilon-toxin, beta-toxin, and netb. ... | 2014 | 26104459 |
| protein- and dna-based anthrax toxin vaccines confer protection in guinea pigs against inhalational challenge with bacillus cereus g9241. | in the past decade, several bacillus cereus strains have been isolated from otherwise healthy individuals who succumbed to bacterial pneumonia presenting symptoms resembling inhalational anthrax. one strain was indistinguishable from b. cereus g9241, previously cultured from an individual who survived a similar pneumonia-like illness and which was shown to possess a complete set of plasmid-borne anthrax toxin-encoding homologs. the finding that b. cereus g9241 pathogenesis in mice is dependent o ... | 2014 | 25044336 |
| decontamination of a bsl3 laboratory by hydrogen peroxide fumigation using three different surrogates for bacillus anthracis spores. | two independent trials investigated the decontamination of a bsl3 laboratory using vaporous hydrogen peroxide and compared the effect on spores of bacillus cereus, bacillus subtilis and bacillus thuringiensis as surrogates for bacillus anthracis spores, while spores of geobacillus stearothermophilus served as control. | 2014 | 25040253 |
| bacillus anthracis-like bacteria and other b. cereus group members in a microbial community within the international space station: a challenge for rapid and easy molecular detection of virulent b. anthracis. | for some microbial species, such as bacillus anthracis, the etiologic agent of the disease anthrax, correct detection and identification by molecular methods can be problematic. the detection of virulent b. anthracis is challenging due to multiple virulence markers that need to be present in order for b. anthracis to be virulent and its close relationship to bacillus cereus and other members of the b. cereus group. this is especially the case in environments where build-up of bacillus spores can ... | 2014 | 24945323 |
| complete genome sequence of bacillus cereus sensu lato bacteriophage bcp1. | bacillus cereus sensu lato organisms are an ecologically diverse group that includes etiologic agents of food poisoning, periodontal disease, and anthrax. the recently identified bcp1 bacteriophage infects b. cereus sensu lato and is being developed as a therapeutic decontamination agent and diagnostic countermeasure. we announce the complete genome sequence of bcp1. | 2014 | 24926042 |
| the genetic diversity of cereulide biosynthesis gene cluster indicates a composite transposon tnces in emetic bacillus weihenstephanensis. | cereulide is a cyclic dodecadepsipeptide ionophore, produced via non-ribosomal peptide synthetases (nrps), which in rare cases can lead to human death. early studies had shown that emetic toxin formation belongs to a homogeneous group of bacillus cereus sensu stricto and the genetic determinants of cereulide (a 24-kb gene cluster of ceshptabcd) are located on a 270-kb plasmid related to the bacillus anthracis virulence plasmid pxo1. | 2014 | 24906385 |
| draft genome sequence of bacillus cereus strain bcfl2013, a clinical isolate similar to g9241. | bacillus cereus strains, such as g9241, causing anthrax-like illnesses have recently been discovered. we report the genome sequence of a clinical strain, b. cereus bcfl2013, which is similar to g9241, recovered from a patient in florida. | 2014 | 24874674 |
| bacillus subtilis systems biology: applications of -omics techniques to the study of endospore formation. | endospore-forming bacteria, with bacillus subtilis being the prevalent model organism, belong to the phylum firmicutes. although the last common ancestor of all firmicutes is likely to have been an endospore-forming species, not every lineage in the phylum has maintained the ability to produce endospores (hereafter, spores). in 1997, the release of the full genome sequence for b. subtilis strain 168 marked the beginning of the genomic era for the study of spore formation (sporulation). in this o ... | 2014 | 26105826 |
| bacillus cereus certhrax adp-ribosylates vinculin to disrupt focal adhesion complexes and cell adhesion. | bacillus cereus is often associated with mild to moderate gastroenteritis; however, some recent isolates cause inhalational anthrax-like diseases and death. these potential emerging human pathogens express multiple virulence factors. b. cereus strain g9241 expresses anthrax toxin, several polysaccharide capsules, and the novel adp-ribosyltransferase, certhrax. in this study, we show that certhrax adp-ribosylates arg-433 of vinculin, a protein that coordinates actin cytoskeleton and extracellular ... | 2014 | 24573681 |
| rapid homogenous time-resolved fluorescence (htrf) immunoassay for anthrax detection. | infection with bacillus anthracsis spores induces an acute anthrax disease that can cause casualties and death in untreated cases. thus rapid diagnosis of anthrax at early stage of the disease is essential to allow an effective treatment. here we present the development of rapid and sensitive homogenous time-resolved fluorescence (htrf) immunoassays based on the energy transfer process of europium cryptate (euk) donor to alexafluor647 acceptor. the energy transfer process is limited to d < 10 nm ... | 2014 | 24515915 |
| inactivation and ultrastructure analysis of bacillus spp. and clostridium perfringens spores. | bacterial endospores are resistant to many environmental factors from temperature extremes to ultraviolet irradiation and are generally more difficult to inactivate or kill than vegetative bacterial cells. it is often considered necessary to treat spores or samples containing spores with chemical fixative solutions for prolonged periods of time (e.g., 1-21 days) to achieve fixation/inactivation to enable electron microscopy (em) examination outside of containment laboratories. prolonged exposure ... | 2014 | 24503289 |
| novel giant siphovirus from bacillus anthracis features unusual genome characteristics. | here we present vb_bans-tsamsa, a novel temperate phage isolated from bacillus anthracis, the agent responsible for anthrax infections in wildlife, livestock and humans. tsamsa phage is a giant siphovirus (order caudovirales), featuring a long, flexible and non-contractile tail of 440 nm (not including baseplate structure) and an isometric head of 82 nm in diameter. we induced tsamsa phage in samples from two different carcass sites in etosha national park, namibia. the tsamsa phage genome is th ... | 2014 | 24475065 |
| the regulated synthesis of a bacillus anthracis spore coat protein that affects spore surface properties. | examine the regulation of a spore coat protein and the effects on spore properties. | 2014 | 24460572 |
| exoproteome analysis of a novel strain of bacillus cereus implicated in disease resembling cutaneous anthrax. | bacillus cereus belongs to b. cereus sensu lato group, shared by six other related species including bacillus anthracis. b. anthracis is the causative agent for serious illness affecting a wide range of animals as well as humans and is a category a biological and toxin warfare (btw) agent. recent studies indicate that a bacillus species other than b. anthracis can cause anthrax-like disease and role of anthrax virulence plasmids (pxo1 and pxo2) on the pathogenicity of b. cereus has been document ... | 2014 | 24412723 |
| identification and validation of specific markers of bacillus anthracis spores by proteomics and genomics approaches. | bacillus anthracis is the causative bacteria of anthrax, an acute and often fatal disease in humans. the infectious agent, the spore, represents a real bioterrorism threat and its specific identification is crucial. however, because of the high genomic relatedness within the bacillus cereus group, it is still a real challenge to identify b. anthracis spores confidently. mass spectrometry-based tools represent a powerful approach to the efficient discovery and identification of such protein marke ... | 2014 | 24379445 |
| the designer proline-rich antibacterial peptide a3-apo prevents bacillus anthracis mortality by deactivating bacterial toxins. | proline-rich antibacterial peptides protect experimental animals from bacterial challenge even if they are unable to kill the microorganisms in vitro. their major in vivo modes of action are inhibition of bacterial protein folding and immunostimulation. here we investigated whether the proline-rich antibacterial peptide dimer a3-apo was able to inhibit bacillus cereus enterotoxin production in vitro and restrict the proliferation of lethal toxin-induced bacillus anthracis replication in mouse ma ... | 2014 | 24164262 |
| the worldwide distribution of genetically and phylogenetically diverse bacillus cereus isolates harbouring bacillus anthracis-like plasmids. | bacillus cereus is a close relative of b. anthracis, the causative agent of anthrax whose pathogenic determinants are located on pxo1 and pxo2 plasmids. bacillus anthracis-like plasmids have been also noted among b. cereus, however, genetic features of b. cereus harbouring these elements remain largely undescribed, especially from the global perspective. herein, we present the genetic polymorphism, population structure and phylogeny of b. cereus with pxo1-/pxo2-like plasmids originating from arg ... | 2015 | 26033739 |
| protein composition of the outermost exosporium-like layer of clostridium difficile 630 spores. | clostridium difficile spores are considered the morphotype of infection, transmission and persistence of c. difficile infections. there is a lack of information on the composition of the outermost exosporium layer of c. difficile spores. using recently developed exosporium removal methods combined with ms/ms, we have established a gel-free approach to analyze the proteome of the exosporium of c. difficile spores of strain 630. a total of 184 proteins were found in the exosporium layer of c. diff ... | 2015 | 25849250 |
| biosynthesis, characterization, and antimicrobial applications of silver nanoparticles. | in the present study, the strain brevibacterium frigoritolerans dc2 was explored for the efficient and extracellular synthesis of silver nanoparticles. these biosynthesized silver nanoparticles were characterized by ultraviolet-visible spectrophotometry, which detected the formation of silver nanoparticles in the reaction mixture and showed a maximum absorbance at 420 nm. in addition, field emission transmission electron microscopy revealed the spherical shape of the nanoparticles. the dynamic l ... | 2015 | 25848272 |
| capsules, toxins and atxa as virulence factors of emerging bacillus cereus biovar anthracis. | emerging b. cereus strains that cause anthrax-like disease have been isolated in cameroon (ca strain) and côte d'ivoire (ci strain). these strains are unusual, because their genomic characterisation shows that they belong to the b. cereus species, although they harbour two plasmids, pbcxo1 and pbcxo2, that are highly similar to the pxo1 and pxo2 plasmids of b. anthracis that encode the toxins and the polyglutamate capsule respectively. the virulence factors implicated in the pathogenicity of the ... | 2015 | 25830379 |
| identification and characterization of a novel geobacillus thermoglucosidasius bacteriophage, gve3. | the study of extremophilic phages may reveal new phage families as well as different mechanisms of infection, propagation and lysis to those found in phages from temperate environments. we describe a novel siphovirus, gve3, which infects the thermophile geobacillus thermoglucosidasius. the genome size is 141,298 bp (g+c 29.6%), making it the largest geobacillus spp-infecting phage known. gve3 appears to be most closely related to the recently described bacillus anthracis phage vb_bans_tsamsa, ra ... | 2015 | 26123922 |
| real-time pcr identification of unique bacillus anthracis sequences. | bacillus anthracis is a spore-forming, gram-positive microorganism. it is a causative agent of anthrax, a highly infectious disease. it belongs to the "bacillus cereus group", which contains other closely related species, including bacillus cereus, bacillus thuringiensis, bacillus mycoides, bacillus weihenstephanensis, and bacillus pseudomycoides. b. anthracis naturally occurs in soil environments. the ba5345 genetic marker was used for highly specific detection of b. anthracis with taqman probe ... | 2015 | 26667574 |
| spore germination. | despite being resistant to a variety of environmental insults, the bacterial endospore can sense the presence of small molecules and respond by germinating, losing the specialized structures of the dormant spore, and resuming active metabolism, before outgrowing into vegetative cells. our current level of understanding of the spore germination process in bacilli and clostridia is reviewed, with particular emphasis on the germinant receptors characterized in bacillus subtilis, bacillus cereus, an ... | 2015 | 27337279 |
| massive horizontal gene transfer, strictly vertical inheritance and ancient duplications differentially shape the evolution of bacillus cereus enterotoxin operons hbl, cytk and nhe. | bacillus cereus sensu lato comprises eight closely related species including the human pathogens bacillus anthracis and bacillus cereus. within b. cereus sensu lato, chromosomally and plasmid-encoded toxins exist. while plasmid-mediated horizontal gene transfer of the emetic toxin, anthrax and insecticidal toxins is known, evolution of enterotoxin genes within the group has not been studied. | 2015 | 26555390 |
| the bacillus anthracis exosporium: what's the big "hairy" deal? | in some bacillus species, including bacillus subtilis, the coat is the outermost layer of the spore. in others, such as the bacillus cereus family, there is an additional layer that envelops the coat, called the exosporium. in the case of bacillus anthracis, a series of fine hair-like projections, also referred to as a "hairy" nap, extends from the exosporium basal layer. the exact role of the exosporium in b. anthracis, or for any of the bacillus species possessing this structure, remains uncle ... | 2015 | 26542035 |
| evaluation of tools for environmental sampling of bacillus anthracis spores. | this study describes the validation of sampling techniques used to detect biological warfare agents used in terror attacks. for this purpose, we tested the efficiencies of different sampling media and extraction solutions for the recovery of bacterial pathogens. we first used bacillus cereus atcc 4342 spores as a surrogate for highly pathogenic b. anthracis to compare recovery efficiencies of spores from four different surfaces. we used three different types of sampling swabs and four different ... | 2015 | 26528669 |
| the exosporium layer of bacterial spores: a connection to the environment and the infected host. | much of what we know regarding bacterial spore structure and function has been learned from studies of the genetically well-characterized bacterium bacillus subtilis. molecular aspects of spore structure, assembly, and function are well defined. however, certain bacteria produce spores with an outer spore layer, the exosporium, which is not present on b. subtilis spores. our understanding of the composition and biological functions of the exosporium layer is much more limited than that of other ... | 2015 | 26512126 |
| a cluster of cns infections due to b. cereus in the setting of acute myeloid leukemia: neuropathology in 5 patients. | bacillus cereus typically causes a self-limited foodborne gastrointestinal (gi) illness. severe invasive infection occurs rarely, mainly among immunocompromised hosts. we describe a cluster of b. cereus infections among 5 patients with acute myeloid leukemia and chemotherapy-induced neutropenia. the initial case presented with occipital lobe abscess and was found on biopsy to have organisms consistent with bacillus species. within 1 week, a second patient died of fulminant brain swelling and hem ... | 2015 | 26352989 |
| reagent-free and portable detection of bacillus anthracis spores using a microfluidic incubator and smartphone microscope. | bacillus anthracis is the causative agent of anthrax and can be contracted by humans and herbivorous mammals by inhalation, ingestion, or cutaneous exposure to bacterial spores. due to its stability and disease potential, b. anthracis is a recognized biothreat agent and robust detection and viability methods are needed to identify spores from unknown samples. here we report the use of smartphone-based microscopy (spm) in combination with a simple microfluidic incubation device (mid) to detect 50 ... | 2015 | 26266749 |
| impacts of sporulation temperature, exposure to compost matrix and temperature on survival of bacillus cereus spores during livestock mortality composting. | to investigate impact of sporulation and compost temperatures on feasibility of composting for disposal of carcasses contaminated with bacillus anthracis. | 2015 | 25580774 |
| cytochrome c551 and the cytochrome c maturation pathway affect virulence gene expression in bacillus cereus atcc 14579. | loss of the cytochrome c maturation system in bacillus cereus results in increased transcription of the major enterotoxin genes nhe, hbl, and cytk and the virulence regulator plcr. increased virulence factor production occurs at 37°c under aerobic conditions, similar to previous findings in bacillus anthracis. unlike b. anthracis, much of the increased virulence gene expression can be attributed to loss of only c551, one of the two small c-type cytochromes. additional virulence factor expression ... | 2015 | 25422307 |
| potentiality of eisenia fetida to degrade disposable paper cups-an ecofriendly solution to solid waste pollution. | the aim of the present study was to subject the post-consumer waste, namely paper cups for vermicomposting along with cow dung in three different ratios for a period of 90-140 days employing eisenia fetida. the post-consumer wastes are a menace in many developing countries including india. this waste was provided as feed for earthworms and was converted to vermicompost. vermicompost prepared with paper cup waste was analyzed for their physicochemical properties. based on the physicochemical prop ... | 2015 | 25220767 |
| bacillus cereus from the environment is genetically related to the highly pathogenic b. cereus in zambia. | to follow-up anthrax in zambia since the outbreak in 2011, we have collected samples from the environment and the carcasses of anthrax-suspected animals, and have tried to isolate bacillus anthracis. in the process of identification of b. anthracis, we collected two isolates, of which colonies were similar to b. anthracis; however, from the results of identification using the molecular-based methods, two isolates were genetically related to the highly pathogenic b. cereus, of which clinical mani ... | 2015 | 25797134 |
| the effect of polyphenols isolated from cynanchi wilfordii radix with anti-inflammatory, antioxidant, and anti-bacterial activity. | recently, cynanchi wilfordii radix has gained wide use in asian countries as a functional food effective for relieving fatigue, osteoporosis, and constipation, particularly in menopausal disorders. however, its anti-inflammatory and anti-microbial activities have not been explored in detail to date. the anti-inflammatory, antioxidant, and anti-bacterial properties of the cynanchi wilfordii radix extracts obtained with water, methanol, ethanol, and acetone were compared. all 4 polyphenol-containi ... | 2015 | 25729277 |
| inactivation of chemical and heat-resistant spores of bacillus and geobacillus by nitrogen cold atmospheric plasma evokes distinct changes in morphology and integrity of spores. | bacterial spores are resistant to severe conditions and form a challenge to eradicate from food or food packaging material. cold atmospheric plasma (cap) treatment is receiving more attention as potential sterilization method at relatively mild conditions but the exact mechanism of inactivation is still not fully understood. in this study, the biocidal effect by nitrogen cap was determined for chemical (hypochlorite and hydrogen peroxide), physical (uv) and heat-resistant spores. the three diffe ... | 2015 | 25481059 |
| antimicrobial activity of neo-clerodane diterpenoids isolated from lamiaceae species against pathogenic and food spoilage microorganisms. | antimicrobial activity of nineteen neo-clerodane diterpenoids, isolated from the acetone extracts of the aerial parts of scutellaria and salvia species (lamiaceae) were tested against thirteen strains belonging to nine different species of pathogenic and food spoilage bacteria aeromonas hydrophila, bacillus cereus, escherichia coli, listeria monocytogenes, proteus vulgaris, pseudomonas aeruginosa, pseudomonas fluorescens, salmonella abony and staphylococcus aureus as well as against two yeast st ... | 2015 | 26749799 |
| screening of microorganisms from deep-sea mud for antarctic krill (euphausia superba) fermentation and evaluation of the bioactive compounds. | twelve kinds of strains were isolated from deep-sea mud which can use antarctic krill powder as the sole carbon/nitrogen source. these strains were identified by 16s rdna sequence analysis and grouped into eight different genera, including bacillus, shewanella, psychrobacter, klebsiella, macrococcus, aeromonas, acinetobacter, and saccharomyces. after fermentation of antarctic krill powder using these strains, bioactive compounds including total phenolics, free amino acids, and enzyme activities ... | 2015 | 25416479 |
| [preliminary analyses on bacterial diversity and resistance in infection-related skin disorders]. | to explore the bacterial diversity and resistance in infection-related skin disorders. | 2015 | 26704163 |
| microbiological stability of canned tuna produced in italy and in non-european countries. | the results of a study on the microbiological stability of canned tuna produced by italian companies and similar canned products manufactured in countries outside europe are reported herein. the study involved 38 samples of canned tuna of various brands, of which 14 were produced by companies outside europe and 24 by italian companies. qualitative and quantitative microbiological tests were conducted for the following parameters: bacterial colony counts at 30°c, total coliforms, total enterobact ... | 2015 | 27800383 |
| bacteria present in comadia redtenbacheri larvae (lepidoptera: cossidae). | the external and internal culturable bacterial community present in the larvae of comadia redtenbacheri hammerschmidt, an edible insect, was studied. characterization of the isolates determined the existence of 18 morphotypes and phylogenetic analysis of the 16s rrna gene revealed the existence of paenibacillus sp., bacillus safensis, pseudomonas sp., bacillus pseudomycoides, corynebacterium variabile, enterococcus sp., gordonia sp., acinetobacter calcoaceticus, arthrobacter sp., micrococcus sp. ... | 2015 | 26336239 |
| antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia). | antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ... | 2015 | 25803299 |
| [screening probiotic endophytic bacteria from medicinal plant flex cornuta and the phytopathogen-inhibiting effect]. | culturable endophytic bacteria were isolated from medicinal plant ilex cornuta by plate-spreading method, strains with strong inhibitory effect on phytopathogen were screened by confrontation culture and fermentation filtrate culture methods, and the morphological changes of phytopathogen hyphae treated with endophytic bacteria were examined by microscopy and micrograph. their phylogenetic relationships were determined by homology analysis of the 16s rdna sequences of pcr products and the taxono ... | 2015 | 26571677 |
| characterization of films based on chitosan lactate and its blends with oxidized starch and gelatin. | minimal inhibitory concentration (mic) of chitosan lactate (chl) was tested against bacteria and phytopathogenic fungi. then, the structural, physicochemical and antimicrobial properties of films based on chl, oxidized potato starch (ops), and gelatin (gel) were investigated. with the exception of rhizopus nigricans, chl was effective against the target organisms. gram-positive bacteria (staphylococcus aureus and bacillus cereus) were more sensitive to chl than gram-negative bacteria (pectobacte ... | 2015 | 25841370 |
| endophytic bacteria from pinellia ternata, a new source of purine alkaloids and bacterial manure. | pinellia ternata (thunb.) berit., a perennial herb belonging to araceae, is one of the few medicinal plants to produce purine alkaloids. it is speculated that endophytic bacteria from p. ternata may produce guanosine or inosine. however, there is no report about endophytic bacteria in p. ternata. | 2015 | 25868618 |
| isolation & identification of bacteria for the treatment of brown crab (cancer pagurus) waste to produce chitinous material. | to isolate bacteria from soil for microbial pretreatment of brown crab (cancer pagurus) shell waste and the production of chitin. | 2015 | 25644656 |
| synthesis and in vitro antimicrobial evaluation of piperazine substituted quinazoline-based thiourea/thiazolidinone/chalcone hybrids. | in frames of the search for new biological entities to fight against recent drug-resistant microbial strains, we report a library of quinazoline-based thiourea/4-thiazolidinone/chalcone hybrids. the newly synthesized compounds were studied for efficacy against several bacteria (staphylococcus aureus, bacillus cereus, pseudomonas aeruginosa, and klebsiella pneumoniae) and fungi (candida albicans and aspergillus clavatus) using the broth dilution technique. from the biological evaluation, (e)-3-(3 ... | 2015 | 26165131 |
| diversity of food-borne bacillus volatile compounds and influence on fungal growth. | to evaluate the antifungal activity of the volatile organic compounds (vocs) produced by 75 different food-borne bacillus species against aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus clavatus, fusarium oxysporum f. sp. lactucae and moniliophthora perniciosa and to determine the vocs responsible for the inhibition. | 2015 | 25989039 |
| a cluster of bacillus cereus bacteremia cases among injection drug users. | bacillus cereus is a ubiquitous spore-forming organism that is infrequently implicated in extraintestinal infections. the authors report three cases of b cereus bacteremia among injection drug users presenting within one month to an urban tertiary care hospital. treatment with intravenous vancomycin was successful in all three cases. while temporal association suggested an outbreak, molecular studies of patient isolates using pulsed-field gel electrophoresis did not suggest a common source. a re ... | 2015 | 26015795 |
| chromosome-directed pcr-based detection and quantification of bacillus cereus group members with focus on b. thuringiensis serovar israelensis active against nematoceran larvae. | bacillus thuringiensis serovar israelensis is a wide-spread soil bacterium affiliated with the b. cereus group (bcg) and is widely used in biocontrol products applied against mosquito and black fly larvae. for monitoring and quantification of applied b. thuringiensis serovar israelensis and its effect on indigenous b. thuringiensis serovar israelensis and bcg assemblages, efficient and reliable tools are essential. the abundance and properties of b. thuringiensis serovar israelensis strains in t ... | 2015 | 25979887 |
| a novel multiplex pcr discriminates bacillus anthracis and its genetically related strains from other bacillus cereus group species. | anthrax is an important zoonotic disease worldwide that is caused by bacillus anthracis, a spore-forming pathogenic bacterium. a rapid and sensitive method to detect b. anthracis is important for anthrax risk management and control in animal cases to address public health issues. however, it has recently become difficult to identify b. anthracis by using previously reported molecular-based methods because of the emergence of b. cereus, which causes severe extra-intestinal infection, as well as t ... | 2015 | 25774512 |
| in vitro evaluation of antimicrobial efficacy of extracts obtained from raw and fermented wild macrofungus, lenzites quercina. | in recent time, there is a major concern about antibiotic resistance displayed by some pathogenic microorganisms and this had involved a continuous search for natural antimicrobial products. the phytochemistry as well as antimicrobial activity of extracts obtained from lenzites quercina was investigated. the extracts and purified fractions were, respectively, tested against indicator organisms using agar well diffusion and disc diffusion methods. the quantity of phytochemicals found in the extra ... | 2015 | 26604928 |
| phylogenetic relationship of phosphate solubilizing bacteria according to 16s rrna genes. | phosphate solubilizing bacteria (psb) can convert insoluble form of phosphorous to an available form. applications of psb as inoculants increase the phosphorus uptake by plant in the field. in this study, isolation and precise identification of psb were carried out in malaysian (serdang) oil palm field (university putra malaysia). identification and phylogenetic analysis of 8 better isolates were carried out by 16s rrna gene sequencing in which as a result five isolates belong to the beta subdiv ... | 2015 | 25632387 |
| brown algae (phaeophyceae) from the coast of madagascar: preliminary bioactivity studies and isolation of natural products. | eight species of brown algae (phaeophyceae) from the coast of madagascar have been investigated for their chemical constituents. fucosterol (3) was obtained as the most abundant compound. the brown alga sargassum ilicifolium was the source for the first isolation of the terpenoid c27-alcohol 1,1',2-trinorsqualenol (1) from marine sources. from s. incisifolium we isolated the highly unsaturated glycolipid 1-o-palmitoyl-2-o-stearidonoyl-3-o-β-d-galactopyranosylglycerol (4) and we report the first ... | 2015 | 26358714 |
| structural characterization and antimicrobial activity of a biosurfactant obtained from bacillus pumilus dsvp18 grown on potato peels. | biosurfactants constitute a structurally diverse group of surface-active compounds derived from microorganisms. they are widely used industrially in various industrial applications such as pharmaceutical and environmental sectors. major limiting factor in biosurfactant production is their production cost. | 2015 | 26495106 |
| red algae (rhodophyta) from the coast of madagascar: preliminary bioactivity studies and isolation of natural products. | several species of red algae (rhodophyta) from the coastal regions of madagascar have been investigated for their natural products. the most abundant compound was cholesterol (5) in combination with a series of oxidized congeners. the brominated indoles 1-3 along with the sesquiterpene debilone (4) have been isolated from laurencia complanata. for the first time, debilone (4) has been obtained from a marine plant. from the methanol extract of calloseris sp., we have achieved the second isolation ... | 2015 | 26198236 |
| microwave assisted one-pot catalyst free green synthesis of new methyl-7-amino-4-oxo-5-phenyl-2-thioxo-2,3,4,5-tetrahydro-1h-pyrano[2,3-d]pyrimidine-6-carboxylates as potent in vitro antibacterial and antifungal activity. | an efficiently simple protocol for the synthesis of methyl 7 amino-4-oxo-5-phenyl-2-thioxo-2, 3, 4,5-tetrahydro-1h-pyrano[2,3-d]pyrimidine-6-carboxylates via one-pot three component condensation pathway is established via microwave irradiation using varied benzaldehyde derivatives, methylcyanoacetate and thio-barbituric acid in water as a green solvent. a variety of functionalized substrates were found to react under this methodology due to its easy operability and offers several advantages like ... | 2015 | 26644932 |
| [differential sensitivity of microorganisms to polyhexamethyleneguanidine]. | factors identified that affect the sensitivity of microorganisms to polyhexamethyleneguanidine (phmg). salts of phmg chloride, valerate, maleate, succinate was to use. test strains of esherichia coli, staphylococcus aureus, bacillus cereus, leptospira interrogans, paenibacillus larvae, mycobacterium bovis, m. avium, m. fortuitum, aspergillus niger and some strains of viruses are taken as objects of research. we have determined that the cytoplasm membrane phospholipids is main "target" for the po ... | 2015 | 26638480 |
| composition and bioactivities of an (e)-β-farnesene chemotype of chamomile (matricaria chamomilla) essential oil from nepal. | the essential oil of matricaria chamomilla, collected from nepal, was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. the major components in nepalese chamomile oil were (e)-β-famesene (42.2%), α-bisabolol oxide a (22.3%), (e,e)-α-famesene (8.3%), cis-bicycloether (5.0%), α-bisabolol oxide b (4.5%), and α-bisabolone oxide a (4.0%). a cluster analysis based on the chemical compositions of 48 samples of chamomile oil reported in the literature has revealed seven ... | 2015 | 26434140 |
| mechanistic aspects of biologically synthesized silver nanoparticles against food- and water-borne microbes. | in the present study, silver nanoparticles (agnps) synthesized from aqueous leaves extract of malva crispa and their mode of interaction with food- and water-borne microbes were investigated. formation of agnps was conformed through uv-vis, fe-sem, eds, afm, and hr-tem analyses. further the concentration of silver (ag) in the reaction mixture was conformed through icp-ms analysis. different concentration of nanoparticles (1-3 mm) tested to know the inhibitory effect of bacterial pathogens such a ... | 2015 | 26178241 |
| ieodoglucomide c and ieodoglycolipid, new glycolipids from a marine-derived bacterium bacillus licheniformis 09idym23. | chemical examination of the ethyl acetate extract from the fermentation broth of the marine-derived bacterium bacillus licheniformis resulted in the isolation of two new glycolipids, ieodoglucomide c (1) and ieodoglycolipid (2). the structural characterization of 1 and 2 was achieved by extensive spectroscopic evidence, including 2d nmr experiments. a combination of chemical derivatization techniques followed by nmr studies, lc-ms data analysis and a literature review was deployed for the establ ... | 2015 | 25893812 |
| secondary metabolites of seseli rigidum: chemical composition plus antioxidant, antimicrobial and cholinesterase inhibition activity. | extracts of different polarity obtained from various plant parts (root, leaf, flower and fruit) of seseli rigidum were studied by different antioxidant assays: dpph and abts radical scavenging activity, by total reducing power method as well as via total content of flavonoids and polyphenols. essential oils of all plant parts showed weak antioxidant characteristics. the inhibitory concentration range of the tested extracts, against bacteria escherichia coli, pseudomonas aeruginosa, staphylococcu ... | 2015 | 25863020 |
| assessment of microbial contamination within working environments of different types of composting plants. | the objective of the study was to determine the degree of microbiological contamination, type of microflora, bioaerosol particle size distribution, and concentration of endotoxins in dust in different types of composting plants. in addition, this study provides a list of indicator microorganisms that pose a biological threat in composting facilities, based on their prevalence within the workplace, source of isolation, and health hazards. we undertook microbiological analysis of the air, work sur ... | 2015 | 25947216 |
| antimicrobial and antiquorum-sensing studies. part 3: synthesis and biological evaluation of new series of [1,3,4]thiadiazoles and fused [1,3,4]thiadiazoles. | new series of [1,3,4]thiadiazoles and fused [1,3,4]thiadiazoles were synthesized. the newly synthesized compounds were screened for their antibacterial activity against staphylococcus aureus, bacillus cereus, and escherichia coli. compounds 3b and 10a displayed the highest activity against e. coli with mic value of 78.125 μg/ml. in addition, compound 10a exhibited the highest activity against b. cereus with mic value of 156.25 μg/ml. the antifungal activity of these compounds was also tested aga ... | 2015 | 25773477 |
| distinguishing the dominant species of pathogen in maxillary sinusitis by sequencing dna dataset analysis. | this study determined the microbial composition in maxillary sinusitis and determined the predominant fungal and bacterial species. samples were obtained from 11 patients with fungal sinusitis and 2 healthy patients. the 13 samples were sequenced using an illumina hi-seq 2000, and mapping to the human, bacterial, and the fungal genomes were based on several steps. bioinformatics and statistical analyses were then performed for bacterial and fungal expressions in the samples. all samples were cul ... | 2015 | 25724392 |
| antimicrobial and inhibition on heat-induced protein denaturation of constituents isolated from polygonatum verticillatum rhizomes. | this study was designed to assess the susceptibility of various microorganisms and inhibition on heat-induced protein denaturation against diosgenin and santonin, isolated from polygonatum verticillatum rhizomes. both diosgenin and santonin showed significant zone of inhibition when studied against various gram-positive (bacillus subtilis, bacillus cereus, staphylococcus aureus and staphylococcus epidermidis) and gram-negative bacteria (escherichia coli and salmonella typhi). in antifungal assay ... | 2015 | 25553804 |
| synthesis, spectroscopic, anticancer, antibacterial and antifungal studies of ni(ii) and cu(ii) complexes with hydrazine carboxamide, 2-[3-methyl-2-thienyl methylene]. | schiff's base ligand(l) hydrazine carboxamide, 2-[3-methyl-2-thienyl methylene] and its metal complexes have been synthesized and characterized by elemental analysis, molar conductance, various spectroscopic techniques such as electronic, ir, (1)h nmr, mass, epr. molar conductance of complexes in dmf solution corresponds to non-electrolyte. complexes have general composition [m(l)2x2], where m=ni(ii) and cu(ii), x=cl(-), no3(-), ch3coo(-) and ½so4(2-). on the basis of above spectral studies, an ... | 2015 | 25087168 |
| chitin extraction from crab shells by bacillus bacteria. biological activities of fermented crab supernatants. | crab shells waste were fermented using six protease-producing bacillus species (bacillus subtilis a26, bacillus mojavensis a21, bacillus pumilus a1, bacillus amyloliquefaciens an6, bacillus licheniformis nh1 and bacillus cereus bg1) for the production of chitin and fermented-crab supernatants (fcss). in medium containing only crab shells, the highest demineralization dm was obtained with b. licheniformis nh1 (83±0.5%) and b. pumilus a1 (80±0.6%), while the highest deproteinization (dp) was achie ... | 2015 | 25910648 |
| characterization of deep sea fish gut bacteria with antagonistic potential, from centroscyllium fabricii (deep sea shark). | the bacterial isolates from centroscyllium fabricii (deep sea shark) gut were screened for antagonistic activity by cross-streak method and disc diffusion assay. this study focuses on strain btss-3, which showed antimicrobial activity against pathogenic bacteria including salmonella typhimurium, proteus vulgaris, clostridium perfringens, staphylococcus aureus, bacillus cereus, bacillus circulans, bacillus macerans and bacillus pumilus. btss3 was subjected to phenotypic characterization using bio ... | 2015 | 25740801 |
| microbial growth under supercritical co2. | growth of microorganisms in environments containing co2 above its critical point is unexpected due to a combination of deleterious effects, including cytoplasmic acidification and membrane destabilization. thus, supercritical co2 (scco2) is generally regarded as a sterilizing agent. we report isolation of bacteria from three sites targeted for geologic carbon dioxide sequestration (gcs) that are capable of growth in pressurized bioreactors containing scco2. analysis of 16s rrna genes from scco2 ... | 2015 | 25681188 |
| identification of single nucleotide polymorphisms (snps) in the 16s rrna gene of foodborne bacillus spp. | the main goal of this work was the identification of single nucleotide polymorphisms (snps) in the 16s rrna gene of foodborne bacillus spp. that may be useful for typing purposes. these species include, among others, bacillus cereus, an important pathogenic species involved in food poisoning, and bacillus licheniformis, bacillus subtilis and bacillus pumilus, which are causative agents of food spoilage described as responsible for foodborne disease outbreaks. with this purpose in mind, 52 bacill ... | 2015 | 25475292 |
| detection of boron removal capacities of different microorganisms in wastewater and effective removal process. | in this study boron removal capacities of different microorganisms were tested. candida tropicalis, rhodotorula mucilaginosa, micrococcus luteus, bacillus thuringiensis, bacillus cereus, bacillus megaterium, bacillus pumilus, pseudomonas aeruginosa and aspergillus versicolor were examined for their boron bioaccumulation capacities in simulated municipal wastewater. a. versicolor and b. cereus were found as the most boron-tolerant microorganisms in the experiments. also boron bioaccumulation yiel ... | 2015 | 26540546 |
| diversity, antimicrobial and antioxidant activities of culturable bacterial endophyte communities in aloe vera. | twenty-nine culturable bacterial endophytes were isolated from surface-sterilized tissues (root, stem and leaf) of aloe vera and molecularly characterized to 13 genera: pseudomonas, bacillus, enterobacter, pantoea, chryseobacterium, sphingobacterium, aeromonas, providencia, cedecea, klebsiella, cronobacter, macrococcus and shigella. the dominant genera include bacillus (20.7%), pseudomonas (20.7%) and enterobacter (13.8%). the crude and ethyl acetate fractions of the metabolites of six isolates, ... | 2015 | 26454221 |
| bacillus cereus atcc 14579 rpon (sigma 54) is a pleiotropic regulator of growth, carbohydrate metabolism, motility, biofilm formation and toxin production. | sigma 54 is a transcriptional regulator predicted to play a role in physical interaction of bacteria with their environment, including virulence and biofilm formation. in order to study the role of sigma 54 in bacillus cereus, a comparative transcriptome and phenotypic study was performed using b. cereus atcc 14579 wt, a markerless rpon deletion mutant, and its complemented strain. the mutant was impaired in many different cellular functions including low temperature and anaerobic growth, carboh ... | 2015 | 26241851 |
| the biosynthesis of udp-d-quinac in bacillus cereus atcc 14579. | n-acetylquinovosamine (2-acetamido-2,6-di-deoxy-d-glucose, quinac) is a relatively rare amino sugar residue found in glycans of few pathogenic gram-negative bacteria where it can play a role in infection. however, little is known about quinac-related polysaccharides in gram-positive bacteria. in a routine screen for bacillus glycan grown at defined medium, it was surprising to identify a quinac residue in polysaccharides isolated from this gram-positive bacterium. to gain insight into the biosyn ... | 2015 | 26207987 |
| proteolytic activities expressed by gastrointestinal pathogens bacillus cereus, listeria monocytogenes and enterococcus faecium in different growth phases. | bacterial proteases are implicated in protein quality control, biofilm formation or might have a direct function in pathogenesis by processing virulence factors or cleaving host factors. in recent years, knowledge of proteases expressed by gram-negative pathogens remarkably increased. however, investigation of proteases from gram-positive bacteria is rather rare, but required for the analysis of pathogenesis-relevant proteases. in this study, we extracted and detected proteases from the gastroin ... | 2015 | 26682199 |
| methylatable signaling helix coordinated inhibitory receiver domain in sensor kinase modulates environmental stress response in bacillus cereus. | σb, an alternative transcription factor, controls the response of the cell to a variety of environmental stresses in bacillus cereus. previously, we reported that rsbm negatively regulates σb through the methylation of rsbk, a hybrid sensor kinase, on a signaling helix (s-helix). however, rsbk comprises a c-terminal receiver (rec) domain whose function remains unclear. in this study, deletion of the c-terminal rec domain of rsbk resulted in high constitutive σb expression independent of environm ... | 2015 | 26379238 |
| multiplex detection of nine food-borne pathogens by mpcr and capillary electrophoresis after using a universal pre-enrichment medium. | routine microbiological quality analyses in food samples require, in some cases, an initial incubation in pre-enrichment medium. this is necessary in order to ensure that small amounts of pathogenic strains are going to be detected. in this work, a universal pre-enrichment medium has been developed for the simultaneous growth of bacillus cereus, campylobacter jejuni, clostridium perfringens, cronobacter sakazakii, escherichia coli, enterobacteriaceae family (38 species, 27 genera), listeria mono ... | 2015 | 26579100 |
| bacteria associated with amblyomma cajennense tick eggs. | ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. in the americas, the ixodidae ticks amblyomma cajennense are responsible for severe impact on livestock and public health. in the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with a. cajennense eggs from females sampled in distinct geographical sites in southeastern brazil. additional comparative analysis of the culturable bacteria from an ... | 2015 | 26537602 |
| inhibition of pathogenic and spoilage bacteria by a novel biofilm-forming lactobacillus isolate: a potential host for the expression of heterologous proteins. | bacterial biofilms are a preferred mode of growth for many types of microorganisms in their natural environments. the ability of pathogens to integrate within a biofilm is pivotal to their survival. the possibility of biofilm formation in lactobacillus communities is also important in various industrial and medical settings. lactobacilli can eliminate the colonization of different pathogenic microorganisms. alternatively, new opportunities are now arising with the rapidly expanding potential of ... | 2015 | 26150120 |
| chemical composition and antimicrobial activity of hexane leaf extract of anisopus mannii (asclepiadaceae). | the aim was to determine the chemical constituents and antimicrobial activity of the hexane leaf extract of anisopus mannii against a wide range of human pathogenic microorganisms. | 2015 | 26401399 |
| three novel lantibiotics, ticins a1, a3, and a4, have extremely stable properties and are promising food biopreservatives. | lantibiotics are antimicrobial peptides with potential applications as the next generation of antimicrobials in the food industry and/or the pharmaceutical industry. nisin has successfully been used as a food preservative for over 40 years, but its major drawback is its limited stability under neutral and alkaline ph conditions. to identify alternatives with better biochemical properties, we screened more than 100 strains of the bacillus cereus group. three novel lantibiotics, ticins a1 (4,062.9 ... | 2015 | 26231642 |
| effects of temperature and ph on the activities of catechol 2,3-dioxygenase obtained from crude oil contaminated soil in ilaje, ondo state, nigeria. | enrichment technique was employed for the isolation of the crude oil degrading bacteria. the isolated bacteria were screened for their degradative ability and the best degrading bacteria were selected based on their growth. specific activities of catechol-2,3-dioxygenase and effects of temperature and ph and their stabilities on the enzyme relative activities were observed. bacteria isolated from the soil sample include; bacillus cereus, b. amyloliquficiens, b. firmus, acinetobacter calcoaceticu ... | 2015 | 26464607 |
| the bacillus cereus group is an excellent reservoir of novel lanthipeptides. | lantibiotics are ribosomally synthesized peptides that contain multiple posttranslational modifications. research on lantibiotics has increased recently, mainly due to their broad-spectrum antimicrobial activity, especially against some clinical gram-positive pathogens. many reports about various bacteriocins in the bacillus cereus group have been published, but few were about lantibiotics. in this study, we identified 101 putative lanthipeptide gene clusters from 77 out of 223 strains of this g ... | 2015 | 25548056 |
| ces locus embedded proteins control the non-ribosomal synthesis of the cereulide toxin in emetic bacillus cereus on multiple levels. | the emetic toxin cereulide produced by bacillus cereus is synthesized by the modular enzyme complex ces that is encoded on a pxo1-like megaplasmid. to decipher the role of the genes adjacent to the structural genes cesa/cesb, coding for the non-ribosomal peptide synthetase (nrps), gene inactivation- and overexpression mutants of the emetic strain f4810/72 were constructed and their impact on cereulide biosynthesis was assessed. the hydrolase cesh turned out to be a part of the complex regulatory ... | 2015 | 26528255 |
| bacillus cereus efflux protein bc3310 - a multidrug transporter of the unknown major facilitator family, umf-2. | phylogenetic classification divides the major facilitator superfamily (mfs) into 82 families, including 25 families that are comprised of transporters with no characterized functions. this study describes functional data for bc3310 from bacillus cereus atcc 14579, a member of the "unknown major facilitator family-2" (umf-2). bc3310 was shown to be a multidrug efflux pump conferring resistance to ethidium bromide, sds and silver nitrate when heterologously expressed in escherichia coli dh5α δacrab. ... | 2015 | 26528249 |
| melanin-like pigment synthesis by soil bacillus weihenstephanensis isolates from northeastern poland. | although melanin is known for protecting living organisms from harmful physical and chemical factors, its synthesis is rarely observed among endospore-forming bacillus cereus sensu lato. here, for the first time, we reported that psychrotolerant bacillus weihenstephanensis from northeastern poland can produce melanin-like pigment. we assessed physicochemical properties of the pigment and the mechanism of its synthesis in relation to b. weihenstephanensis genotypic and phenotypic characteristics. ... | 2015 | 25909751 |
| pcr detection and identification of bacterial contaminants in ocular samples from post-operative endophthalmitis. | bacterial endophthalmitis is a sight-threatening complication of ocular surgery which requires urgent medical consideration including comprehensive diagnosis. polymerase chain reaction (pcr) as a sensitive molecular method has been extensively used for detection of microbial species in clinical specimens. | 2015 | 26023576 |
| infection of cardiovascular implantable electronic devices: detection with sonication, swab cultures, and blood cultures. | the number of patients with cardiovascular implantable electronic devices (cieds) is increasing. the purpose of this study was to compare three methods (sonication, swab cultures, and blood cultures) to detect bacteria in infections of cieds. | 2015 | 25377386 |
| pharmacological basis for the medicinal use of linum usitatissimum (flaxseed) in infectious and non-infectious diarrhea. | linum usitatissimum, commonly known as flaxseed has traditionally been used for the management of diarrhea and gastrointestinal infections. this study was planned to assess pharmacological basis for the medicinal use of flaxseed in infectious and non-infectious diarrhea. | 2015 | 25433250 |
| production and purification of anti-bacterial biometabolite from wild-type lactobacillus, isolated from fermented bamboo shoot: future suggestions and a proposed system for secondary metabolite onsite recovery during continuous fermentation. | wild-type lactobacillus isolated form khorisa, a fermented bamboo shoot product of assam, india were evaluated for production anti-bacterial secondary biometabolites, against staphylococcus aureus. submerged fermentation technique was used for the production of secondary anti-microbial biometabolite by a single wild-type lactobacillus strain, which tested positive for the release of anti-bacterial factor(s). crude cell-free supernatant was obtained, followed by extraction in water-immiscible sol ... | 2015 | 25432345 |